Lag0041473 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0041473
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Rop guanine nucleotide exchange factor
Location	chr13: 18549009 .. 18549793 (+)
RNA-Seq Expression	Lag0041473
Synteny	Lag0041473

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACGAAGGAGAGGTTTGCTAAACTCCTCCTTGGTGAAGACATGTCGGGGGGAGGGAAAGGGGTTTGCACCGCCCTTGCTATCTCAAATGCCATCACCAATCTCTTTGGTAGATCCTCCATTTGAGCCTCTTATTTATTTATTTTTGTAGCGTAAAGCTTATTAACATTTCTGAATACTGTTAGAACATTCTGGTTGGAAATTTCAGCTAGTGTATTTGGTGAATTATGGAGATTGGAGCCACTGGCGCCACAAAAAAGGGCAATGTGACACAGAGAAATGGAATGGCTTTTATGTGTTTGTGATTCCATTGTGGAGCTTGTTTCTACCGTTCAGCCTTTCCCCGGTGGAGGTACTTATGAAGTAATGATGTCTAAACCATGCTTGGATTTGCACATGAACCTCCCTACCTTGGAAGTGCTTGTTGGAGATATGCTCAAATAAGAACTGAGTTACACTAGCAAACTGATTACACCTTAACTGGGTACTAACAATTACAGTGGAAATAACAATACAAACACTGTATATAAGACAAATGCTGTAAAATATATTGACAATAAATAGTCGAGGCACGCTTACAAAGCGCTGTCCTGAAGACAATTATTCGCTTTGCACAGGTTACTAGCAGGCTACAGCGATCGTCTCAGCAGGATACAACGACCAATTTCAGTAGAACAGCAACCTCGAACTACTTGGATCTGACAGAACTCTTGGATACTTGCTCTCAACTTGACTCAAGATCAAAGGAAACAAAGAAAGAAGAAAGAATGCTCCAATTGGAATGA

mRNA sequence

ATGACGAAGGAGAGGTTTGCTAAACTCCTCCTTGGTGAAGACATGTCGGGGGGAGGGAAAGGGGTTTGCACCGCCCTTGCTATCTCAAATGCCATCACCAATCTCTTTGAACATTCTGGTTGGAAATTTCAGCTAGTGTATTTGGTGAATTATGGAGATTGGAGCCACTGGCGCCACAAAAAAGGGCAATGTGACACAGAGAAATGGAATGGCTTTTATGTGTTTGTGATTCCATTGTGGAGCTTGTTTCTACCGTTCAGCCTTTCCCCGGTGGAGGTTACTAGCAGGCTACAGCGATCGTCTCAGCAGGATACAACGACCAATTTCAGTAGAACAGCAACCTCGAACTACTTGGATCTGACAGAACTCTTGGATACTTGCTCTCAACTTGACTCAAGATCAAAGGAAACAAAGAAAGAAGAAAGAATGCTCCAATTGGAATGA

Coding sequence (CDS)

ATGACGAAGGAGAGGTTTGCTAAACTCCTCCTTGGTGAAGACATGTCGGGGGGAGGGAAAGGGGTTTGCACCGCCCTTGCTATCTCAAATGCCATCACCAATCTCTTTGAACATTCTGGTTGGAAATTTCAGCTAGTGTATTTGGTGAATTATGGAGATTGGAGCCACTGGCGCCACAAAAAAGGGCAATGTGACACAGAGAAATGGAATGGCTTTTATGTGTTTGTGATTCCATTGTGGAGCTTGTTTCTACCGTTCAGCCTTTCCCCGGTGGAGGTTACTAGCAGGCTACAGCGATCGTCTCAGCAGGATACAACGACCAATTTCAGTAGAACAGCAACCTCGAACTACTTGGATCTGACAGAACTCTTGGATACTTGCTCTCAACTTGACTCAAGATCAAAGGAAACAAAGAAAGAAGAAAGAATGCTCCAATTGGAATGA

Protein sequence

MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNLFEHSGWKFQLVYLVNYGDWSHWRHKKGQCDTEKWNGFYVFVIPLWSLFLPFSLSPVEVTSRLQRSSQQDTTTNFSRTATSNYLDLTELLDTCSQLDSRSKETKKEERMLQLE

Homology

[Close]

BLAST of Lag0041473 vs. NCBI nr
Match: KAF2318982.1 (hypothetical protein GH714_012143 [Hevea brasiliensis])

HSP 1 Score: 94.7 bits (234), Expect = 6.9e-16
Identity = 46/77 (59.74%), Postives = 54/77 (70.13%), Query Frame = 0


Query: 1   MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNLFEHSGWKFQLVYLVNYGDWSHWRHK 60
           M KERFAKLLLGEDMSG GKGVCTALAISNAITNL         +    N GDWSH   +
Sbjct: 97  MMKERFAKLLLGEDMSGSGKGVCTALAISNAITNLC--------VTIFGNCGDWSHCLQR 156

Query: 61  KGQCDTEKWNGFYVFVI 78
           + QC  E+W+GF+V ++
Sbjct: 157 RNQCGEERWSGFFVSIM 165

[Close]

BLAST of Lag0041473 vs. NCBI nr
Match: RWR90643.1 (rop guanine nucleotide exchange factor 1-like protein [Cinnamomum micranthum f. kanehirae])

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-09
Identity = 38/51 (74.51%), Postives = 39/51 (76.47%), Query Frame = 0


Query: 1   MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNLFEHSGWKFQLVYLVNY 52
           M KERFAKLLLGEDMSGGGKGVCTALAISNAITNL   S +    V L  Y
Sbjct: 98  MMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSGESRFPSPFVTLGTY 148

[Close]

BLAST of Lag0041473 vs. NCBI nr
Match: KAG6570990.1 (Rop guanine nucleotide exchange factor 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-09
Identity = 49/101 (48.51%), Postives = 58/101 (57.43%), Query Frame = 0


Query: 1   MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNLFEH---SGWKFQLVYLVNYGDWSHW 60
           M KERFAKLLLGEDMSGGGKGVCTALAISNAITNL        W+ + +       W   
Sbjct: 108 MMKERFAKLLLGEDMSGGGKGVCTALAISNAITNLSASIFGELWRLEPLAPQKQAMW--- 167

Query: 61  RHKKGQ-----CDTEKWNGFYVFVIPLWSLFLPFSLSPVEV 94
            H++ +     CD+            + SLFL F+LS VEV
Sbjct: 168 -HREMEWLLCVCDS------------MLSLFLLFNLSLVEV 192

[Close]

BLAST of Lag0041473 vs. NCBI nr
Match: KAG0474984.1 (hypothetical protein HPP92_014670 [Vanilla planifolia])

HSP 1 Score: 72.8 bits (177), Expect = 2.8e-09
Identity = 43/91 (47.25%), Postives = 54/91 (59.34%), Query Frame = 0


Query: 3   KERFAKLLLGEDMSGGGKGVCTALAISNAITNLFEHSGWKFQLVYLVNYGDWSHWRHKKG 62
           KERFAKLLLGED SGG KGV TALA+SNAIT+L         +    N G+WS    K+ 
Sbjct: 369 KERFAKLLLGEDPSGGTKGVSTALALSNAITSL--------SVSVFGNSGNWSLCLKKER 428

Query: 63  QCDTEKWNGFYVFVIPLWSLFLPFSLSPVEV 94
              T+KW G ++  I  WS     ++ P+EV
Sbjct: 429 TDGTKKWTGCFLQRIIWWSWCPQSNMGPMEV 451

[Close]

BLAST of Lag0041473 vs. NCBI nr
Match: TYI37851.1 (hypothetical protein ES332_A03G243800v1 [Gossypium tomentosum])

HSP 1 Score: 72.0 bits (175), Expect = 4.8e-09
Identity = 38/59 (64.41%), Postives = 43/59 (72.88%), Query Frame = 0


Query: 1   MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNLFE------HSGWKFQLVYLVNYGD 54
           M KERFAKLLLGED+SGGGKGVCTALAISNAITNL E         WK ++ +L+   D
Sbjct: 95  MMKERFAKLLLGEDLSGGGKGVCTALAISNAITNLSEPLAPQKKEMWKREMEWLLCVSD 153

[Close]

BLAST of Lag0041473 vs. ExPASy Swiss-Prot
Match: Q93ZY2 (Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF1 PE=1 SV=2)

HSP 1 Score: 70.5 bits (171), Expect = 1.8e-11
Identity = 34/35 (97.14%), Postives = 34/35 (97.14%), Query Frame = 0


Query: 1   MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNL 36
           M KERFAKLLLGEDMSGGGKGVCTALAISNAITNL
Sbjct: 93  MMKERFAKLLLGEDMSGGGKGVCTALAISNAITNL 127

[Close]

BLAST of Lag0041473 vs. ExPASy Swiss-Prot
Match: F4K295 (Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF5 PE=2 SV=1)

HSP 1 Score: 68.2 bits (165), Expect = 9.0e-11
Identity = 33/35 (94.29%), Postives = 33/35 (94.29%), Query Frame = 0


Query: 1   MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNL 36
           M KERFAKLLLGEDMSG GKGVCTALAISNAITNL
Sbjct: 105 MMKERFAKLLLGEDMSGSGKGVCTALAISNAITNL 139

[Close]

BLAST of Lag0041473 vs. ExPASy Swiss-Prot
Match: Q9LZN0 (Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF7 PE=1 SV=1)

HSP 1 Score: 65.1 bits (157), Expect = 7.7e-10
Identity = 31/35 (88.57%), Postives = 32/35 (91.43%), Query Frame = 0


Query: 1   MTKERFAKLLLGEDMSGGGKGVCTALAISNAITNL 36
           M KERF+KLLLGEDMSG G GVCTALAISNAITNL
Sbjct: 73  MMKERFSKLLLGEDMSGSGNGVCTALAISNAITNL 107

[Close]

BLAST of Lag0041473 vs. ExPASy Swiss-Prot
Match: A4IJ27 (Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF3 PE=2 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.0e-09
Identity = 30/34 (88.24%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 3   KERFAKLLLGEDMSGGGKGVCTALAISNAITNLF 37
           KERFAKLLLGEDMSG GKGVCTA+ ISNAITNL+
Sbjct: 109 KERFAKLLLGEDMSGSGKGVCTAVTISNAITNLY 142

[Close]

BLAST of Lag0041473 vs. ExPASy Swiss-Prot
Match: Q9LQ89 (Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana OX=3702 GN=ROPGEF2 PE=1 SV=2)

HSP 1 Score: 63.5 bits (153), Expect = 2.2e-09
Identity = 29/34 (85.29%), Postives = 32/34 (94.12%), Query Frame = 0


Query: 3   KERFAKLLLGEDMSGGGKGVCTALAISNAITNLF 37
           KERF+KLLLGEDMSG GKGVCTA+ ISNAITNL+
Sbjct: 121 KERFSKLLLGEDMSGSGKGVCTAVTISNAITNLY 154

The following BLAST results are available for this feature:

BLAST of Lag0041473 vs. NCBI nr


Match Name	E-value	Identity	Description
KAF2318982.1	6.9e-16	59.74	hypothetical protein GH714_012143 [Hevea brasiliensis]	[more]
RWR90643.1	2.8e-09	74.51	rop guanine nucleotide exchange factor 1-like protein [Cinnamomum micranthum f. ...	[more]
KAG6570990.1	2.8e-09	48.51	Rop guanine nucleotide exchange factor 1, partial [Cucurbita argyrosperma subsp....	[more]
KAG0474984.1	2.8e-09	47.25	hypothetical protein HPP92_014670 [Vanilla planifolia]	[more]
TYI37851.1	4.8e-09	64.41	hypothetical protein ES332_A03G243800v1 [Gossypium tomentosum]	[more]

BLAST of Lag0041473 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93ZY2	1.8e-11	97.14	Rop guanine nucleotide exchange factor 1 OS=Arabidopsis thaliana OX=3702 GN=ROPG...	[more]
F4K295	9.0e-11	94.29	Rop guanine nucleotide exchange factor 5 OS=Arabidopsis thaliana OX=3702 GN=ROPG...	[more]
Q9LZN0	7.7e-10	88.57	Rop guanine nucleotide exchange factor 7 OS=Arabidopsis thaliana OX=3702 GN=ROPG...	[more]
A4IJ27	1.0e-09	88.24	Rop guanine nucleotide exchange factor 3 OS=Arabidopsis thaliana OX=3702 GN=ROPG...	[more]
Q9LQ89	2.2e-09	85.29	Rop guanine nucleotide exchange factor 2 OS=Arabidopsis thaliana OX=3702 GN=ROPG...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005512	PRONE domain	PFAM	PF03759	PRONE	coord: 1..36 e-value: 2.2E-17 score: 62.9
IPR005512	PRONE domain	PROSITE	PS51334	PRONE	coord: 1..147 score: 17.951962
None	No IPR available	GENE3D	1.20.58.2010	PRONE domain, subdomain 1	coord: 1..142 e-value: 2.6E-19 score: 71.4
None	No IPR available	PANTHER	PTHR33101:SF1	ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 5	coord: 1..37
IPR038937	Rop guanine nucleotide exchange factor	PANTHER	PTHR33101	ROP GUANINE NUCLEOTIDE EXCHANGE FACTOR 1	coord: 1..37

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0041473.1	Lag0041473.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016310	phosphorylation
biological_process	GO:0050790	regulation of catalytic activity
molecular_function	GO:0005085	guanyl-nucleotide exchange factor activity
molecular_function	GO:0016301	kinase activity