Lag0040908 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0040908
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPhosphoinositide phospholipase C
Locationchr13: 9651180 .. 9654792 (+)
RNA-Seq ExpressionLag0040908
SyntenyLag0040908
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCCAAGCAGACCTACAGAGTGTGCTTCTGCTTCCGCCGGCGGTTCAAGATGACCGCCGGCGAGGCCCCCGACGAGATCAAGAAGCTCTTCGAGCAGTACTCCGAGAATGGGATCATGAACGCCGATCATCTCCACCGATTCTTGGTCGAAGTTCAGAAGGAGGTCAAGACGACGAGGGACGACGCTCAGAACATGATTGAGCGCTGTACTAACGAGCTCAAGCACTTGAACATCTTCCATCGGAAGGTCCTCAATCTTGAAGCCTTCTTCAAGTACCTCTTCAGCGATCTTAACCCTCCGCTCGAGTCTCTTGGGGTAATTTCTGCCTTTCCCCCTCACTCTGCTCACTCTTGATTATTGCTTTAGATTTTAGCTTCTTTTGTTTCTTTCTATGTTTTTTTGGCTTTTGGATGGATGAGTGGTGTTACTGTCAATTGTGTGTGGAAATTAATTAATCTCTGAATCCCCTTTTGTGTTCTTGGATTGTATTGAAAGGGATCCCGGATCTCTCTCTCTCTCTCTTTGTGCGTGTCGGTGTGTGTTTCTATATCAAATATAATGTTTGTTTTCAGCTTTTTGTGGGGCAAAGCATCTTGTTACTTTGTAAGTTGTACGAGGATAAGTTCGGCATTATCACTGAACATGGAATGGGATATATTCTGTTTTTCAGGTGCACCAAGATATGAGTGCTCCTTTATCGCATTATTACATATATACAGGTCACAACTCGTACTTGACTGGGAATCAGCTCAGCAGTGACTGCAGTGATGTGCCCATCATTCAAGCCCTGCAGAGAGGAGTTAGAGTAATTGAATTGGATATATGGCCAAATTCATCAAAGGATGATATAGATGTTCTTCATGGCAGGTGTTCATTTGTATCTCCTTTCCTTTTCTTTCAGTAATGGGCATCATCTGTTTTAACTTTTAAGTGGAATTTCTTTTTTTCCTTTGTAAATCCTGATTGTGAGATTGCTTTCAGGACACTTACTACCCCAGTGGAGCTCATTAAATGTTTGAAATCTATTAAGGAGCATGCCTTTGAAACATCTGAATATCCAGTTGTAATAACATTGGAGGACCATCTTCCTCCTAACCTTCAAGCTAAAGTGGCTGAGGTGCGCACTGGTTTAGTTTTTCTTGCACCTCCCCGATTGGTGTATTTTAAGATCTTATATAATATATAATTTTCTCAACTGTTCTAGATGGTAACAAATACATTTGGAGACATTTTGTTCTCTCCTGGCTCTGAATGCTTGAAGGAATTTCCTTCTCCTGAATCACTGAAGAAAAGGATTATTATATCGACTAAACCACCTAAGGAGTACCTTAAGACCAAAGATGCTCCTAAGGAGGGTGAGACACAGAAGGAAAAGACTTCTGGTGAAGATAATAAAGAAACATCAGCATGGGGAAGAGAAGTGTCAAGCTTGAAAGGTGGCACTGTAACTGACTACAAGGTCTGTGATGTTGCCCATGAATTTTATCATCCTTATAATTTTTAAAACAGGTTTTGATCTAATGCTTGGTTGGTTCTGCTTCACAGCAAGATTTGGATGAAGATAGTAATGATGAGGAAGATCCTGAGGATGGAGATACCAAATCTACAGACTCTGCACAGTATATTGCACCTGAATATAAAAATTTGATTGCTATTCATGCGGGAAAGCCTAAAGGTGGAATTGATGCATGCCTCAAAGTGGATCCCAACAAAGTTAGACGCTTGAGCTTGAGTGAGCAACAATTGGAAAAGGCAGTGCTTACTCATGGAAAACAAATTGTTAGGTATGAACAAAATCTGCTTCTCAATTGAAGACATTCATAGTATGTAGTGTCTAGTCAATGATCTCTGACAAGCAGGCGTCTAAATGGGCTATGTAAGCATACATTTCAATGGGCTTGCATTTAGGAGGTCCAAATTCTTTTGAAGTTGTGATTATTAGAAAGGTCAGAGTCAGTGTGCATAAAGTCATAGTCTTTTCATTGGTTTAGGAACAGAAATTTTTGGAGTCTAAATGCCCTAAAAATTCAGTGACTGTAATTATATGGATGAATAGGTAGGGATTAGGATGGTGCAGTTTTTGTTAATTTGTCTACCACTTTAAGCAGAGAACTGGGGCGGGTAGAGAAGTTTGTATTTAATGTGATATACGATAATGAAAAAGATAATTTTCTGCTTTTTTCTTTTTTCCTTATTGGAGTAACATTGTTACAGAGTTTATATCTTCACGTTGTTCATATGTTGTCTCTCTGGGTTACTCATTCTCGGGGCAAATTTTGTTATTTTTCTTACGTTTTCCCTTCTTACTTGCTGTAGGTTTACTCAGCAGAACATTTTGAGAGTCTATCCAAAGGGGATCAGGTTTGACTCATCCAATTATAACCCAATGATTGGGTGGACTCATGGTGCTCAAATGGTTGCACTGAACATGCAGGTCATTTCGTCTTCTTCCTTACATTGAATCGGAGATTTAGATATGGATTGTTTAATTACAAAATTTCTTTGATTCATTCTTTCCCATTTTTCTCTTGTTTGTTAGGAAGAAGAATGTTTGAGCTCTCGCAGTTTGAGATGAAAGATTAGATAGTATCTTAACTTGTTGCTAACCAGCTAGTTGGATAATATCATATGAAACATAAGAACCCCTTCCTAAACATTTGGCTGCTGACTAGAAATGGGATTCAGTTTCATTTTCACTAGTTAACATATCCAATGATCCAATTTTCATAATCTAATCATTTCGTTTCTTTTGATTCGTTCATGATTTGTTTCTCCAATTTTCCAACCACTGAAAATTGGATCATCTGTGAATTATAACAGGGTTATGGAAGGTCACTATGGTTGATGCATGGGATGTTCAGAGCTAATGGTGGGTGTGGTTATGTGAAGAAACCAGATTTCATGTTGAAGCCCGGTTCAGATGATGAGATCTTTGATCCTAAAGTTAGGTTGAATGTGAAAACAACGTTGAAAGTAAGTGTTCTGCAACTTTTTTCCCCTCTCATGACTGTGCTTCATTTTCCTATAGCCAATGTTTGAAATGTAAGCGTACATTGGCTTATTTAATTCGACCATACTGACAATGTTGGTTCTATTTTCTGTTGAGAAGGTGACTGTGTATATGGGAGAAGGATGGTATTATGATTTTCATCACACTCACTTTGATGCTTACTCCCCGCCAGACTTTTACACGAGGGTATGTCAATGAAGTTCACAATATTTTGATTGAATATAGCTAAAACCTTTAGTTTTTCGATTCAAAAAGTAGCTAAAATCTGTTTTTGCTGTGCTCGGGATCGTATGATTATATATGCAGGTGGGAATTGCTGGCGTTCCAGCCGATACTGTAATGAGGAAAACAAAAACATTAGAGGATAACTGGGTGCCTGCTTGGAATGAGGAGTTTGAGTTTCCATTATCTGTTCCAGAACTCGCTTTGCTTCGAATCGAAGTTCACGAGTACGACATGTCTGAAAAGGATGATTTCGGGGGCCAGACATGCCTGCCTGTTGCAGAACTAAGAAGAGGGATTCGAGCAGTTCCACTCCACAACGTCAAGGGAGAGAAGTACAAATCAGTCAAGCTTCTGATGGGGTTTGAATTTGTTTGA

mRNA sequence

ATGTCCAAGCAGACCTACAGAGTGTGCTTCTGCTTCCGCCGGCGGTTCAAGATGACCGCCGGCGAGGCCCCCGACGAGATCAAGAAGCTCTTCGAGCAGTACTCCGAGAATGGGATCATGAACGCCGATCATCTCCACCGATTCTTGGTCGAAGTTCAGAAGGAGGTCAAGACGACGAGGGACGACGCTCAGAACATGATTGAGCGCTGTACTAACGAGCTCAAGCACTTGAACATCTTCCATCGGAAGGTCCTCAATCTTGAAGCCTTCTTCAAGTACCTCTTCAGCGATCTTAACCCTCCGCTCGAGTCTCTTGGGGTGCACCAAGATATGAGTGCTCCTTTATCGCATTATTACATATATACAGGTCACAACTCGTACTTGACTGGGAATCAGCTCAGCAGTGACTGCAGTGATGTGCCCATCATTCAAGCCCTGCAGAGAGGAGTTAGAGTAATTGAATTGGATATATGGCCAAATTCATCAAAGGATGATATAGATGTTCTTCATGGCAGGACACTTACTACCCCAGTGGAGCTCATTAAATGTTTGAAATCTATTAAGGAGCATGCCTTTGAAACATCTGAATATCCAGTTGTAATAACATTGGAGGACCATCTTCCTCCTAACCTTCAAGCTAAAGTGGCTGAGATGGTAACAAATACATTTGGAGACATTTTGTTCTCTCCTGGCTCTGAATGCTTGAAGGAATTTCCTTCTCCTGAATCACTGAAGAAAAGGATTATTATATCGACTAAACCACCTAAGGAGTACCTTAAGACCAAAGATGCTCCTAAGGAGGGTGAGACACAGAAGGAAAAGACTTCTGGTGAAGATAATAAAGAAACATCAGCATGGGGAAGAGAAGTGTCAAGCTTGAAAGGTGGCACTGTAACTGACTACAAGCAAGATTTGGATGAAGATAGTAATGATGAGGAAGATCCTGAGGATGGAGATACCAAATCTACAGACTCTGCACAGTATATTGCACCTGAATATAAAAATTTGATTGCTATTCATGCGGGAAAGCCTAAAGGTGGAATTGATGCATGCCTCAAAGTGGATCCCAACAAAGTTAGACGCTTGAGCTTGAGTGAGCAACAATTGGAAAAGGCAGTGCTTACTCATGGAAAACAAATTGTTAGGTTTACTCAGCAGAACATTTTGAGAGTCTATCCAAAGGGGATCAGGTTTGACTCATCCAATTATAACCCAATGATTGGGTGGACTCATGGTGCTCAAATGGTTGCACTGAACATGCAGGGTTATGGAAGGTCACTATGGTTGATGCATGGGATGTTCAGAGCTAATGGTGGGTGTGGTTATGTGAAGAAACCAGATTTCATGTTGAAGCCCGGTTCAGATGATGAGATCTTTGATCCTAAAGTTAGGTTGAATGTGAAAACAACGTTGAAAGTGACTGTGTATATGGGAGAAGGATGGTATTATGATTTTCATCACACTCACTTTGATGCTTACTCCCCGCCAGACTTTTACACGAGGGTGGGAATTGCTGGCGTTCCAGCCGATACTGTAATGAGGAAAACAAAAACATTAGAGGATAACTGGGTGCCTGCTTGGAATGAGGAGTTTGAGTTTCCATTATCTGTTCCAGAACTCGCTTTGCTTCGAATCGAAGTTCACGAGTACGACATGTCTGAAAAGGATGATTTCGGGGGCCAGACATGCCTGCCTGTTGCAGAACTAAGAAGAGGGATTCGAGCAGTTCCACTCCACAACGTCAAGGGAGAGAAGTACAAATCAGTCAAGCTTCTGATGGGGTTTGAATTTGTTTGA

Coding sequence (CDS)

ATGTCCAAGCAGACCTACAGAGTGTGCTTCTGCTTCCGCCGGCGGTTCAAGATGACCGCCGGCGAGGCCCCCGACGAGATCAAGAAGCTCTTCGAGCAGTACTCCGAGAATGGGATCATGAACGCCGATCATCTCCACCGATTCTTGGTCGAAGTTCAGAAGGAGGTCAAGACGACGAGGGACGACGCTCAGAACATGATTGAGCGCTGTACTAACGAGCTCAAGCACTTGAACATCTTCCATCGGAAGGTCCTCAATCTTGAAGCCTTCTTCAAGTACCTCTTCAGCGATCTTAACCCTCCGCTCGAGTCTCTTGGGGTGCACCAAGATATGAGTGCTCCTTTATCGCATTATTACATATATACAGGTCACAACTCGTACTTGACTGGGAATCAGCTCAGCAGTGACTGCAGTGATGTGCCCATCATTCAAGCCCTGCAGAGAGGAGTTAGAGTAATTGAATTGGATATATGGCCAAATTCATCAAAGGATGATATAGATGTTCTTCATGGCAGGACACTTACTACCCCAGTGGAGCTCATTAAATGTTTGAAATCTATTAAGGAGCATGCCTTTGAAACATCTGAATATCCAGTTGTAATAACATTGGAGGACCATCTTCCTCCTAACCTTCAAGCTAAAGTGGCTGAGATGGTAACAAATACATTTGGAGACATTTTGTTCTCTCCTGGCTCTGAATGCTTGAAGGAATTTCCTTCTCCTGAATCACTGAAGAAAAGGATTATTATATCGACTAAACCACCTAAGGAGTACCTTAAGACCAAAGATGCTCCTAAGGAGGGTGAGACACAGAAGGAAAAGACTTCTGGTGAAGATAATAAAGAAACATCAGCATGGGGAAGAGAAGTGTCAAGCTTGAAAGGTGGCACTGTAACTGACTACAAGCAAGATTTGGATGAAGATAGTAATGATGAGGAAGATCCTGAGGATGGAGATACCAAATCTACAGACTCTGCACAGTATATTGCACCTGAATATAAAAATTTGATTGCTATTCATGCGGGAAAGCCTAAAGGTGGAATTGATGCATGCCTCAAAGTGGATCCCAACAAAGTTAGACGCTTGAGCTTGAGTGAGCAACAATTGGAAAAGGCAGTGCTTACTCATGGAAAACAAATTGTTAGGTTTACTCAGCAGAACATTTTGAGAGTCTATCCAAAGGGGATCAGGTTTGACTCATCCAATTATAACCCAATGATTGGGTGGACTCATGGTGCTCAAATGGTTGCACTGAACATGCAGGGTTATGGAAGGTCACTATGGTTGATGCATGGGATGTTCAGAGCTAATGGTGGGTGTGGTTATGTGAAGAAACCAGATTTCATGTTGAAGCCCGGTTCAGATGATGAGATCTTTGATCCTAAAGTTAGGTTGAATGTGAAAACAACGTTGAAAGTGACTGTGTATATGGGAGAAGGATGGTATTATGATTTTCATCACACTCACTTTGATGCTTACTCCCCGCCAGACTTTTACACGAGGGTGGGAATTGCTGGCGTTCCAGCCGATACTGTAATGAGGAAAACAAAAACATTAGAGGATAACTGGGTGCCTGCTTGGAATGAGGAGTTTGAGTTTCCATTATCTGTTCCAGAACTCGCTTTGCTTCGAATCGAAGTTCACGAGTACGACATGTCTGAAAAGGATGATTTCGGGGGCCAGACATGCCTGCCTGTTGCAGAACTAAGAAGAGGGATTCGAGCAGTTCCACTCCACAACGTCAAGGGAGAGAAGTACAAATCAGTCAAGCTTCTGATGGGGTTTGAATTTGTTTGA

Protein sequence

MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTRDDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYIYTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVELIKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPSPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTDYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV
Homology
BLAST of Lag0040908 vs. NCBI nr
Match: XP_038892600.1 (phosphoinositide phospholipase C 2-like [Benincasa hispida])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 564/598 (94.31%), Postives = 583/598 (97.49%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFE+YSENGIMN DHL RFL++VQKEVKTTR
Sbjct: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEEYSENGIMNPDHLQRFLIDVQKEVKTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVH DMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHHDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKD+IDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDNIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKE+AF  SEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS
Sbjct: 181 IKCLKSIKEYAFVASEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTD 300
           PESLKKRIIISTKPPKEYL+T D PKEGE QKEKTSG+D KETSAWGREVSSLKG +VTD
Sbjct: 241 PESLKKRIIISTKPPKEYLRTNDVPKEGEAQKEKTSGDD-KETSAWGREVSSLKGASVTD 300

Query: 301 YKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVR 360
           YKQDLD+DSNDEED EDGD KST  +QYIAPEYKNLIAIHAGKPKGGIDACLKVDP+KVR
Sbjct: 301 YKQDLDDDSNDEEDTEDGDPKST--SQYIAPEYKNLIAIHAGKPKGGIDACLKVDPDKVR 360

Query: 361 RLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM 420
           RLSLSEQQLEKAVLTHGKQIVRFTQ+NILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM
Sbjct: 361 RLSLSEQQLEKAVLTHGKQIVRFTQKNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM 420

Query: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEG 480
           QGYGRSLWLMHGMFRANGGCGYVKKPDF+LKPGSDDEIFDP+VRL+VKTTLKVTVYMGEG
Sbjct: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFILKPGSDDEIFDPRVRLSVKTTLKVTVYMGEG 480

Query: 481 WYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPEL 540
           WYYDFHHTHFDAYSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNE FEFPL+VPEL
Sbjct: 481 WYYDFHHTHFDAYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEVFEFPLTVPEL 540

Query: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ALLR+EVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV
Sbjct: 541 ALLRVEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 595

BLAST of Lag0040908 vs. NCBI nr
Match: KAG6600927.1 (Phosphoinositide phospholipase C 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 560/598 (93.65%), Postives = 574/598 (95.99%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRF+MTA EAP EIK LF+QYSENGIM+ADHL RFL++VQKEVKTTR
Sbjct: 1   MSKQTYRVCFCFRRRFRMTASEAPKEIKNLFDQYSENGIMDADHLQRFLIDVQKEVKTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLES GVHQDMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESRGVHQDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKEHAF  SEYPVVITLEDHLPPNLQAKVAEMVT+TF DILFSPGSECLKEFPS
Sbjct: 181 IKCLKSIKEHAFVASEYPVVITLEDHLPPNLQAKVAEMVTSTFQDILFSPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTD 300
           PESLKKRIIISTKPPKEYLKTKD PKEGE QKEKTSGE NKET AWGREVSSLK GTVTD
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDVPKEGEPQKEKTSGE-NKETPAWGREVSSLKDGTVTD 300

Query: 301 YKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVR 360
           YKQDLDEDSNDEEDPEDGD KS D  QYIAPEYKNLIAIHAGKPKGGIDACLKVDP KVR
Sbjct: 301 YKQDLDEDSNDEEDPEDGDPKSADCTQYIAPEYKNLIAIHAGKPKGGIDACLKVDPEKVR 360

Query: 361 RLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM 420
           RLSLSEQQLEKA+LTHGKQIVRFTQ+NILR+YPKG RFDSSNYNPMIGWTHGAQMVALNM
Sbjct: 361 RLSLSEQQLEKAILTHGKQIVRFTQRNILRIYPKGTRFDSSNYNPMIGWTHGAQMVALNM 420

Query: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEG 480
           QGYGRSLWLMHGMFRANGGCGYVKKPDF+L PGSDDEIFDP+  L VKTTLKVTVYMGEG
Sbjct: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFILNPGSDDEIFDPRASLPVKTTLKVTVYMGEG 480

Query: 481 WYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPEL 540
           WYYDFHHTHFD YSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPLSVPEL
Sbjct: 481 WYYDFHHTHFDVYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLSVPEL 540

Query: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIR+VPLHNVKGEKYKSVKLLMGFEFV
Sbjct: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRSVPLHNVKGEKYKSVKLLMGFEFV 597

BLAST of Lag0040908 vs. NCBI nr
Match: XP_031741682.1 (phosphoinositide phospholipase C 2 [Cucumis sativus] >KGN50656.1 hypothetical protein Csa_005831 [Cucumis sativus])

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 560/600 (93.33%), Postives = 584/600 (97.33%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLF++YSENGIMN DHL RFL++VQKE  TTR
Sbjct: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFDEYSENGIMNPDHLQRFLIDVQKEANTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVH DMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHHDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKD+IDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDNIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKE+AF  S+YPVVITLEDHLPPNLQAKVAEMV +TFGDILF+PGSECLKEFPS
Sbjct: 181 IKCLKSIKEYAFVASDYPVVITLEDHLPPNLQAKVAEMVMSTFGDILFTPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPK--EGETQKEKTSGEDNKETSAWGREVSSLKGGTV 300
           PESLKKRIIISTKPPKEYLKTKDAPK  EGE QKEKTSG + KE+SAWGREVSSLKGGTV
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGEGEAQKEKTSG-NGKESSAWGREVSSLKGGTV 300

Query: 301 TDYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNK 360
           TDYKQDLD+DSNDEED EDGD KST+S+QYIAPEYK+LIAIHAGKPKGGIDACLKVDPNK
Sbjct: 301 TDYKQDLDDDSNDEEDNEDGDPKSTESSQYIAPEYKSLIAIHAGKPKGGIDACLKVDPNK 360

Query: 361 VRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVAL 420
           VRRLSLSEQQLEKAVLTHGKQIVRFTQ+NILRVYPKGIRFDSSNYNPMIGWTHGAQMVAL
Sbjct: 361 VRRLSLSEQQLEKAVLTHGKQIVRFTQKNILRVYPKGIRFDSSNYNPMIGWTHGAQMVAL 420

Query: 421 NMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMG 480
           NMQGYGRSLW+MHGMFRANGGCGYVKKPDF+LKPGSDDEIFDP+VRL VKTTLKVTVYMG
Sbjct: 421 NMQGYGRSLWIMHGMFRANGGCGYVKKPDFILKPGSDDEIFDPRVRLTVKTTLKVTVYMG 480

Query: 481 EGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVP 540
           EGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPL+VP
Sbjct: 481 EGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLTVP 540

Query: 541 ELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ELALLRIEVHEYDMSEKDDFGGQTCLPV+ELRRGIRAVPLH+VKGEKYKSVKLLMGFEFV
Sbjct: 541 ELALLRIEVHEYDMSEKDDFGGQTCLPVSELRRGIRAVPLHSVKGEKYKSVKLLMGFEFV 599

BLAST of Lag0040908 vs. NCBI nr
Match: XP_023534552.1 (phosphoinositide phospholipase C 2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1151.3 bits (2977), Expect = 0.0e+00
Identity = 559/598 (93.48%), Postives = 573/598 (95.82%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRF+MTA EAP EIK LF+QYSENGIM+ADHL RFL++VQKEVKTTR
Sbjct: 1   MSKQTYRVCFCFRRRFRMTASEAPKEIKNLFDQYSENGIMDADHLQRFLIDVQKEVKTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRK LNLEAFFKYLFSDLNPPLES GVHQDMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKALNLEAFFKYLFSDLNPPLESRGVHQDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKEHAF  SEYPVVITLEDHLPPNLQAKVAEMVT+TF DILFSPGS+CLKEFPS
Sbjct: 181 IKCLKSIKEHAFVASEYPVVITLEDHLPPNLQAKVAEMVTSTFQDILFSPGSDCLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTD 300
           PESLKKRIIISTKPPKEYLKTKD PKEGE QKEKTSGE NKET AWGREVSSLK GTVTD
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDVPKEGEPQKEKTSGE-NKETPAWGREVSSLKDGTVTD 300

Query: 301 YKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVR 360
           YKQDLDEDSNDEEDPEDGD KS D  QYIAPEYKNLIAIHAGKPKGGIDACLKVDP KVR
Sbjct: 301 YKQDLDEDSNDEEDPEDGDPKSADCTQYIAPEYKNLIAIHAGKPKGGIDACLKVDPEKVR 360

Query: 361 RLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM 420
           RLSLSEQQLEKAVLTHGKQIVRFTQ+NILR+YPKG RFDSSNYNPMIGWTHGAQMVALNM
Sbjct: 361 RLSLSEQQLEKAVLTHGKQIVRFTQRNILRIYPKGTRFDSSNYNPMIGWTHGAQMVALNM 420

Query: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEG 480
           QGYGRSLWLMHGMFRANGGCGYVKKPDF+L PGSDDEIFDP+  L VKTTLKVTVYMGEG
Sbjct: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFILNPGSDDEIFDPRASLPVKTTLKVTVYMGEG 480

Query: 481 WYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPEL 540
           WYYDFHHTHFD YSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPLSVPEL
Sbjct: 481 WYYDFHHTHFDVYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLSVPEL 540

Query: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIR+VPLHNVKGEKYKSVKLLMGFEFV
Sbjct: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRSVPLHNVKGEKYKSVKLLMGFEFV 597

BLAST of Lag0040908 vs. NCBI nr
Match: XP_022956857.1 (phosphoinositide phospholipase C 2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 559/598 (93.48%), Postives = 573/598 (95.82%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRF+MTA EAP EIK LF+QYSENGIM+ADHL RFL++VQKEVKTTR
Sbjct: 1   MSKQTYRVCFCFRRRFRMTASEAPKEIKNLFDQYSENGIMDADHLQRFLIDVQKEVKTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLES GVHQDMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESRGVHQDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKEHAF  SEYPVVITLEDHLPPNLQAKVAEMVT+TF DILFSPGSECLKEFPS
Sbjct: 181 IKCLKSIKEHAFVASEYPVVITLEDHLPPNLQAKVAEMVTSTFQDILFSPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTD 300
           PESLKKRIIISTKPPKEYLKTKD PKEGE QKEKTSGE NKET AWGREVSSLK GTVTD
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDVPKEGEPQKEKTSGE-NKETPAWGREVSSLKDGTVTD 300

Query: 301 YKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVR 360
           YKQDLDEDSNDEEDPEDGD KS D  QYIAPEYKNLIAIHAGKPKGGIDACLKVDP KVR
Sbjct: 301 YKQDLDEDSNDEEDPEDGDPKSADCTQYIAPEYKNLIAIHAGKPKGGIDACLKVDPEKVR 360

Query: 361 RLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM 420
           RLSLSEQQLEKA+LTHGKQIVRFTQ+NILR+YPKG R DSSNYNPMIGWTHGAQMVALNM
Sbjct: 361 RLSLSEQQLEKAILTHGKQIVRFTQRNILRIYPKGTRVDSSNYNPMIGWTHGAQMVALNM 420

Query: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEG 480
           QGYGRSLWLMHGMFRANGGCGYVKKPDF+L PGSDDEIFDP+  L VKTTLKVTVYMGEG
Sbjct: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFILTPGSDDEIFDPRASLPVKTTLKVTVYMGEG 480

Query: 481 WYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPEL 540
           WYYDFHHTHFD YSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPLSVPEL
Sbjct: 481 WYYDFHHTHFDVYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLSVPEL 540

Query: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIR+VPLHNVKGEKYKSVKLLMGFEFV
Sbjct: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRSVPLHNVKGEKYKSVKLLMGFEFV 597

BLAST of Lag0040908 vs. ExPASy Swiss-Prot
Match: Q39033 (Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 SV=1)

HSP 1 Score: 865.9 bits (2236), Expect = 2.6e-250
Identity = 424/599 (70.78%), Postives = 490/599 (81.80%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTY+VCFCFRRRF+ TA EAP EIK +FE+YSENG+M  DHLHRFL++VQK+ K TR
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           +DAQ++I   +      ++ HR  L+L+AFFKYLF D NPPL    VH DM AP+SHY+I
Sbjct: 61  EDAQSIINSAS------SLLHRNGLHLDAFFKYLFGDNNPPLALHKVHHDMDAPISHYFI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           +TGHNSYLTGNQLSSDCS+VPII AL++GVRVIELDIWPNS+KDDIDVLHG TLTTPV L
Sbjct: 121 FTGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPG-SECLKEFP 240
           IKCLK+I+ HAF+ S+YPVV+TLEDHL P+LQ+KVAEMVT  FG+ILF+P   E LKEFP
Sbjct: 181 IKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFP 240

Query: 241 SPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVT 300
           SP SLK+RIIISTKPPKEY       KEG+  +    G+D  +   WGREV S      +
Sbjct: 241 SPNSLKRRIIISTKPPKEY-------KEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKS 300

Query: 301 DYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKV 360
           + K DL  D ND++D +D + KS  +A    P+YK+LIAIHAGKPKGGI  CLKVDP+KV
Sbjct: 301 EAKDDL--DGNDDDDDDDDEDKSKINA---PPQYKHLIAIHAGKPKGGITECLKVDPDKV 360

Query: 361 RRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALN 420
           RRLSLSE+QLEKA   + KQIVRFTQ N+LR+YPKG R  SSNYNP++GW+HGAQMVA N
Sbjct: 361 RRLSLSEEQLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFN 420

Query: 421 MQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGE 480
           MQGYGRSLWLM GMFRANGGCGY+KKPD +LK GSD +IFDPK  L VKTTL+VTVYMGE
Sbjct: 421 MQGYGRSLWLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGE 480

Query: 481 GWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPE 540
           GWY+DF HTHFD YSPPDFYTRVGIAGVP DTVM+KTKTLEDNW+PAW+E FEFPL+VPE
Sbjct: 481 GWYFDFRHTHFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPE 540

Query: 541 LALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           LALLR+EVHEYDMSEKDDFGGQTCLPV EL  GIRA PLH+ KGEKYKSVKLL+  EFV
Sbjct: 541 LALLRLEVHEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEFV 581

BLAST of Lag0040908 vs. ExPASy Swiss-Prot
Match: Q9LY51 (Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 SV=1)

HSP 1 Score: 816.2 bits (2107), Expect = 2.4e-235
Identity = 408/603 (67.66%), Postives = 481/603 (79.77%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTY+VCFCFRRR++ T   AP EIK LF+ YS+ G+M  D L RFL++VQK+ K T+
Sbjct: 1   MSKQTYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKGLMTTDLLLRFLIDVQKQDKATK 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           ++AQ+++   +      ++ HR  L+L+AFFKYLF+  N PL SL VHQDM APLSHY+I
Sbjct: 61  EEAQDIVNASS------SLLHRNGLHLDAFFKYLFAVTNSPLSSLEVHQDMDAPLSHYFI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCS++PII+AL++GVRVIELDIWPNS +D IDVLHGRTLT+PVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGS-ECLKEFP 240
           IKCL++I+EHAF+ S+YPVV+TLEDHL P LQAKVAEMVT+ FG++LF+P S ECLKEFP
Sbjct: 181 IKCLRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFP 240

Query: 241 SPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSL----KG 300
           SP  LKKRI+ISTKPPKEY   K A  +   +K    G D  +   WGREV S     + 
Sbjct: 241 SPAFLKKRIMISTKPPKEY---KAATDDDLVKK----GRDLGDKEVWGREVPSFIRRDRS 300

Query: 301 GTVTDYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVD 360
               D   D D+D +D++D +DGD K   +A    PEYK+LIAI AGKPKGGI  CLKVD
Sbjct: 301 VDKNDSNGDDDDDDDDDDDDDDGDDKIKKNA---PPEYKHLIAIEAGKPKGGITECLKVD 360

Query: 361 PNKVRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQM 420
           P+KVRRLSLSE+QLEKA   + KQIVRFTQ+N+LRVYPKG R  SSNYNP+I W+HGAQM
Sbjct: 361 PDKVRRLSLSEEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQM 420

Query: 421 VALNMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTV 480
           VA NMQG GRSLW+M GMFR NGGCGY+KKPD +LK    + +FDP+  L VKTTL+VT+
Sbjct: 421 VAFNMQGLGRSLWVMQGMFRGNGGCGYIKKPDLLLK---SNAVFDPEATLPVKTTLRVTI 480

Query: 481 YMGEGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPL 540
           YMGEGWYYDF HTHFD YSPPDFYTRVGIAGVPADTVM+KTKTLEDNW+PAW+E FEFPL
Sbjct: 481 YMGEGWYYDFPHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPL 540

Query: 541 SVPELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGF 599
           +VPELALLRIEVHEYDMSEKDDFGGQ CLPV ELR+GIRAVPL N  G K +SVKLL+  
Sbjct: 541 TVPELALLRIEVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVRL 584

BLAST of Lag0040908 vs. ExPASy Swiss-Prot
Match: Q8GV43 (Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 SV=2)

HSP 1 Score: 692.6 bits (1786), Expect = 4.0e-198
Identity = 355/611 (58.10%), Postives = 441/611 (72.18%), Query Frame = 0

Query: 6   YRVCFCFRRRFKMTAGEAPDEIKKLFEQYS-----------------ENGIMNADHLHRF 65
           YR+   + R+FK+      D+++  F Q++                   G+M A+ L  F
Sbjct: 19  YRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLCSF 78

Query: 66  LVEVQKEVKTTRDDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLF-SDLNPPLESLGV 125
           L +  +   TT  +AQ +I+       H+  F R  L+L+ FF +LF  DLNPP+ +  V
Sbjct: 79  LDDHGE--STTVAEAQRLIDEVIRRRHHVTRFTRHGLDLDDFFNFLFYDDLNPPI-TPHV 138

Query: 126 HQDMSAPLSHYYIYTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDID 185
           HQDM+APLSHY+IYTGHNSYLTGNQLSSDCS+VP+I+ALQRGVRVIELD+WPNS+  DI+
Sbjct: 139 HQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDIN 198

Query: 186 VLHGRTLTTPVELIKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDIL 245
           VLHGRTLTTPV L+KCLKSI+++AF +S YPV+ITLEDHL P+LQAKVAEM T  FG +L
Sbjct: 199 VLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFGQML 258

Query: 246 FSPGSECLKEFPSPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWG 305
           + P S+ L EFPSP SL  RIIISTKPPKEYL++++   + +      S ED    +   
Sbjct: 259 YYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRT--- 318

Query: 306 REVSSLKGGTV-TDYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKG 365
            E+ +L+      D++   D D  DEE  ED             P YK LI IHAGKPKG
Sbjct: 319 EEIQTLESMLFDQDFESKSDSDQEDEEASEDQ-----------KPAYKRLITIHAGKPKG 378

Query: 366 GIDACLKVDPNKVRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPM 425
            +   +KV  +KVRRLSLSEQ+L++   ++ + +VRFTQ+N+LR+YPKG RF+SSNY P+
Sbjct: 379 TVKEEMKVVVDKVRRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPL 438

Query: 426 IGWTHGAQMVALNMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLN 485
           IGWTHGAQM+A NMQGYG+SLWLMHGMFRANGGCGYVKKP+F++K G  DE+FDP+ +L 
Sbjct: 439 IGWTHGAQMIAFNMQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLP 498

Query: 486 VKTTLKVTVYMGEGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPA 545
           VK TLKV VYMG+GW  DF HTHFDAYSPPDFYT++ I GVPAD   +KTK +EDNW P 
Sbjct: 499 VKETLKVKVYMGDGWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPI 558

Query: 546 WNEEFEFPLSVPELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKY 598
           W+EEF FPL+VPELALLRIEV EYDMSEKDDFGGQTCLPVAELR GIR+VPL++ KGEK 
Sbjct: 559 WDEEFSFPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKM 612

BLAST of Lag0040908 vs. ExPASy Swiss-Prot
Match: Q944C1 (Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 SV=2)

HSP 1 Score: 686.4 bits (1770), Expect = 2.9e-196
Identity = 344/601 (57.24%), Postives = 438/601 (72.88%), Query Frame = 0

Query: 5   TYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSE-NGIMNADHLHRFLVEVQKEVKTTRDDA 64
           +Y+ C  F R+F+MT     ++++ LFE+Y+E +  M+ + L + + E   E +T+ ++A
Sbjct: 9   SYKFCLIFTRKFRMTESGPVEDVRDLFEKYTEGDAHMSPEQLQKLMTEEGGEGETSLEEA 68

Query: 65  QNMIERCTNELKHLNIFHRKVLNLEAFFKYLFS-DLNPPLESLGVHQDMSAPLSHYYIYT 124
           + +++       H+  F R+ L L+ F  YLFS DLNPP+    VHQ+M APLSHY+I+T
Sbjct: 69  ERIVDEVLRRKHHIAKFTRRNLTLDDFNYYLFSTDLNPPIAD-QVHQNMDAPLSHYFIFT 128

Query: 125 GHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVELIK 184
           GHNSYLTGNQLSS+CS++PI  AL+RGVRV+ELD+WP  + DD+ V HGRTLT  V+L K
Sbjct: 129 GHNSYLTGNQLSSNCSELPIADALRRGVRVVELDLWPRGT-DDVCVKHGRTLTKEVKLGK 188

Query: 185 CLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPSPE 244
           CL+SIK +AF  S+YPV+ITLEDHL P LQ KVA+M+T TFGD+L+   S+  +EFPSPE
Sbjct: 189 CLESIKANAFAISKYPVIITLEDHLTPKLQFKVAKMITQTFGDMLYYHDSQGCQEFPSPE 248

Query: 245 SLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTDYK 304
            LK++I+ISTKPPKEYL+  D  ++   +K K S ED      WG+E   L        +
Sbjct: 249 ELKEKILISTKPPKEYLEANDTKEKDNGEKGKDSDED-----VWGKEPEDL-----ISTQ 308

Query: 305 QDLDEDSN-----DEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPN 364
            DLD+ ++     +++D E G  +S  S Q  APEYK LIAIHAGKPKGG+   LKVDPN
Sbjct: 309 SDLDKVTSSVNDLNQDDEERGSCESDTSCQLQAPEYKRLIAIHAGKPKGGLRMALKVDPN 368

Query: 365 KVRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVA 424
           K+RRLSLSEQ LEKAV ++G  ++RFTQ+N LR+YPKG RF+SSNY P IGW  GAQM+A
Sbjct: 369 KIRRLSLSEQLLEKAVASYGADVIRFTQKNFLRIYPKGTRFNSSNYKPQIGWMSGAQMIA 428

Query: 425 LNMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYM 484
            NMQGYGR+LWLM GMFRANGGCGYVKKPDF++    + + F PK   + K TLKV V M
Sbjct: 429 FNMQGYGRALWLMEGMFRANGGCGYVKKPDFLMDASPNGQDFYPKDNSSPKKTLKVKVCM 488

Query: 485 GEGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSV 544
           G+GW  DF  THFD+YSPPDF+ RVGIAG P D VM KTK   D W P WN+EF FPL+V
Sbjct: 489 GDGWLLDFKKTHFDSYSPPDFFVRVGIAGAPVDEVMEKTKIEYDTWTPIWNKEFTFPLAV 548

Query: 545 PELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEF 599
           PELALLR+EVHE+D++EKDDFGGQTCLPV+E+R+GIRAVPL N KG KY S +LLM FEF
Sbjct: 549 PELALLRVEVHEHDVNEKDDFGGQTCLPVSEIRQGIRAVPLFNRKGVKYSSTRLLMRFEF 597

BLAST of Lag0040908 vs. ExPASy Swiss-Prot
Match: Q944C2 (Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 SV=2)

HSP 1 Score: 632.1 bits (1629), Expect = 6.4e-180
Identity = 321/596 (53.86%), Postives = 419/596 (70.30%), Query Frame = 0

Query: 5   TYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSE-NGIMNADHLHRFLVEVQKEVKTTRDDA 64
           +Y++  C   + +M  G  P ++   F +Y+E    M A+ L RFLVEVQ E +    DA
Sbjct: 7   SYKMGLCCSDKLRMNRGAPPQDVVTAFVEYTEGRSHMTAEQLCRFLVEVQDETEVLVSDA 66

Query: 65  QNMIERCTNELKHLNIFHRKVLNLEAFFKYLFS-DLNPPLESLGVHQDMSAPLSHYYIYT 124
           + +IER T E  H+  F R  LNL+ FF +LFS DLN P++S  VHQDM++PLSHY+IYT
Sbjct: 67  EKIIERITCERHHITKFLRHTLNLDDFFSFLFSDDLNHPIDS-KVHQDMASPLSHYFIYT 126

Query: 125 GHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVELIK 184
            HNSYLTGNQ++S+CSDVP+I+AL+RGVR +ELD+WPNS+KDDI VLHG   T PVEL+K
Sbjct: 127 SHNSYLTGNQINSECSDVPLIKALKRGVRALELDMWPNSTKDDILVLHGWAWTPPVELVK 186

Query: 185 CLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPSPE 244
           CL+SIKEHAF  S YPV++TLEDHL P+LQAK AEM+   F D+++ P +  LKEFPSPE
Sbjct: 187 CLRSIKEHAFYASAYPVILTLEDHLTPDLQAKAAEMMKEIFMDMVYFPEAGGLKEFPSPE 246

Query: 245 SLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTDYK 304
            LK +I+ISTKPPK           G  +K+K S     E+ A G+  S      V+   
Sbjct: 247 DLKYKIVISTKPPK-----------GSLRKDKDS-----ESDASGKASSD-----VSADD 306

Query: 305 QDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVRRL 364
           +  +E++++ ++ EDG  + + +  ++   Y  LI I +G  K G+   L +D   VRRL
Sbjct: 307 EKTEEETSEAKNEEDGFDQESSNLDFLT--YSRLITIPSGNAKNGLKEALTIDNGGVRRL 366

Query: 365 SLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNMQG 424
           SL EQ+ +KA   +G ++++FTQ+N+LR+YPK  R +SSNY P  GW +GAQMVA NMQG
Sbjct: 367 SLREQKFKKATEMYGTEVIKFTQKNLLRIYPKATRVNSSNYRPYNGWMYGAQMVAFNMQG 426

Query: 425 YGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEGWY 484
           YGR+LW+MHGMFR NGGCGYVKKPDFM+      E+F+PK +L +K TLKV VYMG+GW 
Sbjct: 427 YGRALWMMHGMFRGNGGCGYVKKPDFMMNNNLSGEVFNPKAKLPIKKTLKVKVYMGKGWD 486

Query: 485 YDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPELAL 544
             F  T F+ +S P+FYTRVGI GV  D VM+KTK  +  W P WNEEFEF L+VPELAL
Sbjct: 487 SGFQRTCFNTWSSPNFYTRVGITGVRGDKVMKKTKKEQKTWEPFWNEEFEFQLTVPELAL 546

Query: 545 LRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           LRIEVH+Y+M EKDDF GQTCLPV+ELR+GIR+VPL++ KGE+  SV LLM F F+
Sbjct: 547 LRIEVHDYNMPEKDDFSGQTCLPVSELRQGIRSVPLYDRKGERLVSVTLLMRFHFL 578

BLAST of Lag0040908 vs. ExPASy TrEMBL
Match: A0A0A0KSE1 (Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_5G203390 PE=4 SV=1)

HSP 1 Score: 1153.7 bits (2983), Expect = 0.0e+00
Identity = 560/600 (93.33%), Postives = 584/600 (97.33%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLF++YSENGIMN DHL RFL++VQKE  TTR
Sbjct: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFDEYSENGIMNPDHLQRFLIDVQKEANTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVH DMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHHDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKD+IDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDNIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKE+AF  S+YPVVITLEDHLPPNLQAKVAEMV +TFGDILF+PGSECLKEFPS
Sbjct: 181 IKCLKSIKEYAFVASDYPVVITLEDHLPPNLQAKVAEMVMSTFGDILFTPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPK--EGETQKEKTSGEDNKETSAWGREVSSLKGGTV 300
           PESLKKRIIISTKPPKEYLKTKDAPK  EGE QKEKTSG + KE+SAWGREVSSLKGGTV
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGEGEAQKEKTSG-NGKESSAWGREVSSLKGGTV 300

Query: 301 TDYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNK 360
           TDYKQDLD+DSNDEED EDGD KST+S+QYIAPEYK+LIAIHAGKPKGGIDACLKVDPNK
Sbjct: 301 TDYKQDLDDDSNDEEDNEDGDPKSTESSQYIAPEYKSLIAIHAGKPKGGIDACLKVDPNK 360

Query: 361 VRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVAL 420
           VRRLSLSEQQLEKAVLTHGKQIVRFTQ+NILRVYPKGIRFDSSNYNPMIGWTHGAQMVAL
Sbjct: 361 VRRLSLSEQQLEKAVLTHGKQIVRFTQKNILRVYPKGIRFDSSNYNPMIGWTHGAQMVAL 420

Query: 421 NMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMG 480
           NMQGYGRSLW+MHGMFRANGGCGYVKKPDF+LKPGSDDEIFDP+VRL VKTTLKVTVYMG
Sbjct: 421 NMQGYGRSLWIMHGMFRANGGCGYVKKPDFILKPGSDDEIFDPRVRLTVKTTLKVTVYMG 480

Query: 481 EGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVP 540
           EGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPL+VP
Sbjct: 481 EGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLTVP 540

Query: 541 ELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ELALLRIEVHEYDMSEKDDFGGQTCLPV+ELRRGIRAVPLH+VKGEKYKSVKLLMGFEFV
Sbjct: 541 ELALLRIEVHEYDMSEKDDFGGQTCLPVSELRRGIRAVPLHSVKGEKYKSVKLLMGFEFV 599

BLAST of Lag0040908 vs. ExPASy TrEMBL
Match: A0A6J1GXJ2 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111458425 PE=4 SV=1)

HSP 1 Score: 1150.6 bits (2975), Expect = 0.0e+00
Identity = 559/598 (93.48%), Postives = 573/598 (95.82%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRF+MTA EAP EIK LF+QYSENGIM+ADHL RFL++VQKEVKTTR
Sbjct: 1   MSKQTYRVCFCFRRRFRMTASEAPKEIKNLFDQYSENGIMDADHLQRFLIDVQKEVKTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLES GVHQDMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESRGVHQDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKEHAF  SEYPVVITLEDHLPPNLQAKVAEMVT+TF DILFSPGSECLKEFPS
Sbjct: 181 IKCLKSIKEHAFVASEYPVVITLEDHLPPNLQAKVAEMVTSTFQDILFSPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTD 300
           PESLKKRIIISTKPPKEYLKTKD PKEGE QKEKTSGE NKET AWGREVSSLK GTVTD
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDVPKEGEPQKEKTSGE-NKETPAWGREVSSLKDGTVTD 300

Query: 301 YKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVR 360
           YKQDLDEDSNDEEDPEDGD KS D  QYIAPEYKNLIAIHAGKPKGGIDACLKVDP KVR
Sbjct: 301 YKQDLDEDSNDEEDPEDGDPKSADCTQYIAPEYKNLIAIHAGKPKGGIDACLKVDPEKVR 360

Query: 361 RLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM 420
           RLSLSEQQLEKA+LTHGKQIVRFTQ+NILR+YPKG R DSSNYNPMIGWTHGAQMVALNM
Sbjct: 361 RLSLSEQQLEKAILTHGKQIVRFTQRNILRIYPKGTRVDSSNYNPMIGWTHGAQMVALNM 420

Query: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEG 480
           QGYGRSLWLMHGMFRANGGCGYVKKPDF+L PGSDDEIFDP+  L VKTTLKVTVYMGEG
Sbjct: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFILTPGSDDEIFDPRASLPVKTTLKVTVYMGEG 480

Query: 481 WYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPEL 540
           WYYDFHHTHFD YSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPLSVPEL
Sbjct: 481 WYYDFHHTHFDVYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLSVPEL 540

Query: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIR+VPLHNVKGEKYKSVKLLMGFEFV
Sbjct: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRSVPLHNVKGEKYKSVKLLMGFEFV 597

BLAST of Lag0040908 vs. ExPASy TrEMBL
Match: A0A6J1GXM8 (Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111458425 PE=4 SV=1)

HSP 1 Score: 1146.0 bits (2963), Expect = 0.0e+00
Identity = 559/599 (93.32%), Postives = 573/599 (95.66%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRF+MTA EAP EIK LF+QYSENGIM+ADHL RFL++VQKEVKTTR
Sbjct: 1   MSKQTYRVCFCFRRRFRMTASEAPKEIKNLFDQYSENGIMDADHLQRFLIDVQKEVKTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLES GVHQDMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESRGVHQDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKEHAF  SEYPVVITLEDHLPPNLQAKVAEMVT+TF DILFSPGSECLKEFPS
Sbjct: 181 IKCLKSIKEHAFVASEYPVVITLEDHLPPNLQAKVAEMVTSTFQDILFSPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTD 300
           PESLKKRIIISTKPPKEYLKTKD PKEGE QKEKTSGE NKET AWGREVSSLK GTVTD
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDVPKEGEPQKEKTSGE-NKETPAWGREVSSLKDGTVTD 300

Query: 301 YK-QDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKV 360
           YK QDLDEDSNDEEDPEDGD KS D  QYIAPEYKNLIAIHAGKPKGGIDACLKVDP KV
Sbjct: 301 YKQQDLDEDSNDEEDPEDGDPKSADCTQYIAPEYKNLIAIHAGKPKGGIDACLKVDPEKV 360

Query: 361 RRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALN 420
           RRLSLSEQQLEKA+LTHGKQIVRFTQ+NILR+YPKG R DSSNYNPMIGWTHGAQMVALN
Sbjct: 361 RRLSLSEQQLEKAILTHGKQIVRFTQRNILRIYPKGTRVDSSNYNPMIGWTHGAQMVALN 420

Query: 421 MQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGE 480
           MQGYGRSLWLMHGMFRANGGCGYVKKPDF+L PGSDDEIFDP+  L VKTTLKVTVYMGE
Sbjct: 421 MQGYGRSLWLMHGMFRANGGCGYVKKPDFILTPGSDDEIFDPRASLPVKTTLKVTVYMGE 480

Query: 481 GWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPE 540
           GWYYDFHHTHFD YSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPLSVPE
Sbjct: 481 GWYYDFHHTHFDVYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLSVPE 540

Query: 541 LALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           LALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIR+VPLHNVKGEKYKSVKLLMGFEFV
Sbjct: 541 LALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRSVPLHNVKGEKYKSVKLLMGFEFV 598

BLAST of Lag0040908 vs. ExPASy TrEMBL
Match: A0A6J1IPZ6 (Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111477133 PE=4 SV=1)

HSP 1 Score: 1145.6 bits (2962), Expect = 0.0e+00
Identity = 557/598 (93.14%), Postives = 572/598 (95.65%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTYRVCFCFRRRF+MTA EAP EIK LF+QYSENGIM+ADHL RFL++VQKEVKTTR
Sbjct: 1   MSKQTYRVCFCFRRRFRMTASEAPKEIKNLFDQYSENGIMDADHLQRFLIDVQKEVKTTR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           DDAQNMIERCTNELKHLNIFHRK LNLEAFFKYLFSDLNPPLES GVHQDMSAPLSHYYI
Sbjct: 61  DDAQNMIERCTNELKHLNIFHRKALNLEAFFKYLFSDLNPPLESRGVHQDMSAPLSHYYI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPS 240
           IKCLKSIKE+AF  SEYPVVITLEDHLPPNLQAKVAEMVT+TF DILFSPGSECLKEFPS
Sbjct: 181 IKCLKSIKEYAFVASEYPVVITLEDHLPPNLQAKVAEMVTSTFQDILFSPGSECLKEFPS 240

Query: 241 PESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVTD 300
           PESLKKRIIISTKPPKEYLKTKD PKEGE QKEKTSGE NKET AWGREVSSLK GTVTD
Sbjct: 241 PESLKKRIIISTKPPKEYLKTKDVPKEGEPQKEKTSGE-NKETPAWGREVSSLKDGTVTD 300

Query: 301 YKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVR 360
           YKQDLDEDSNDE+DPE GD KS D  QYIAPEYKNLIAIHAGKPKGGIDACLKVDP KVR
Sbjct: 301 YKQDLDEDSNDEDDPEYGDPKSADCTQYIAPEYKNLIAIHAGKPKGGIDACLKVDPEKVR 360

Query: 361 RLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNM 420
           RLSLSEQQLEKAVLTHGKQIVRFTQ+NILR+YPKG RFDSSNYNPMIGWTHGAQMVALNM
Sbjct: 361 RLSLSEQQLEKAVLTHGKQIVRFTQRNILRIYPKGTRFDSSNYNPMIGWTHGAQMVALNM 420

Query: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEG 480
           QGYGRSLWLMHGMFRANGGCGYVKKPDF+L PGSDDEIFDP+  L VKTTLKVTVYMGEG
Sbjct: 421 QGYGRSLWLMHGMFRANGGCGYVKKPDFILNPGSDDEIFDPRASLPVKTTLKVTVYMGEG 480

Query: 481 WYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPEL 540
           WYYDFHHTHFD YSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPLSVPEL
Sbjct: 481 WYYDFHHTHFDVYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLSVPEL 540

Query: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIR+VPLHNVKGEKYKSVKLLMGFEFV
Sbjct: 541 ALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRSVPLHNVKGEKYKSVKLLMGFEFV 597

BLAST of Lag0040908 vs. ExPASy TrEMBL
Match: A0A1S3CKB3 (Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103501442 PE=4 SV=1)

HSP 1 Score: 1144.0 bits (2958), Expect = 0.0e+00
Identity = 555/596 (93.12%), Postives = 579/596 (97.15%), Query Frame = 0

Query: 5   TYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTRDDAQ 64
           TYRVCFCFRRRFKMTAGEAPDEIKKLF++YSENGIMN DHL RFL++VQKEV TTRDDAQ
Sbjct: 9   TYRVCFCFRRRFKMTAGEAPDEIKKLFDEYSENGIMNPDHLQRFLIDVQKEVNTTRDDAQ 68

Query: 65  NMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYIYTGH 124
           NMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVH DMSAPLSHYYIYTGH
Sbjct: 69  NMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHHDMSAPLSHYYIYTGH 128

Query: 125 NSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVELIKCL 184
           NSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKD+IDVLHGRTLTTPVELIKCL
Sbjct: 129 NSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDNIDVLHGRTLTTPVELIKCL 188

Query: 185 KSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGSECLKEFPSPESL 244
           KSIKE+AF  S+YPVVITLEDHLPPNLQAKVAEMVT+TFGDILFSPG ECLKEFPSPESL
Sbjct: 189 KSIKEYAFVASDYPVVITLEDHLPPNLQAKVAEMVTSTFGDILFSPGPECLKEFPSPESL 248

Query: 245 KKRIIISTKPPKEYLKTKDAPKEG--ETQKEKTSGEDNKETSAWGREVSSLKGGTVTDYK 304
           KKRIIISTKPPKEYLKTKD PKEG  E+QKEK SG +NKETSAWGREVSSLKGGTVTDYK
Sbjct: 249 KKRIIISTKPPKEYLKTKDVPKEGESESQKEKNSG-NNKETSAWGREVSSLKGGTVTDYK 308

Query: 305 QDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKVRRL 364
           QDLD+DSNDEED EDGD KST+S+ YIAPEYK+LIAIHAGKPKGGIDACL+VDP+K RRL
Sbjct: 309 QDLDDDSNDEEDTEDGDPKSTESSHYIAPEYKSLIAIHAGKPKGGIDACLRVDPDKARRL 368

Query: 365 SLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNMQG 424
           SLSEQQLEKAVLTHGKQIVRFTQ+NILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNMQG
Sbjct: 369 SLSEQQLEKAVLTHGKQIVRFTQKNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALNMQG 428

Query: 425 YGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGEGWY 484
           YGRSLW+MHGMFRANGGCGYVKKPDF+LKPGSDDEIFDPKVRL VKTTLKVTVYMGEGWY
Sbjct: 429 YGRSLWIMHGMFRANGGCGYVKKPDFILKPGSDDEIFDPKVRLPVKTTLKVTVYMGEGWY 488

Query: 485 YDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPELAL 544
           YDFHHTHFDAYSPPDFYTRVGIAGVPADT+M+KTKTLEDNWVPAWNEEFEFPL+VPELAL
Sbjct: 489 YDFHHTHFDAYSPPDFYTRVGIAGVPADTIMKKTKTLEDNWVPAWNEEFEFPLTVPELAL 548

Query: 545 LRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           LRIEVHEYDMSEKDDFGGQTCLPV+ELRRGIRAVPLH+VKGEKYKSVKLLMGFEFV
Sbjct: 549 LRIEVHEYDMSEKDDFGGQTCLPVSELRRGIRAVPLHSVKGEKYKSVKLLMGFEFV 603

BLAST of Lag0040908 vs. TAIR 10
Match: AT3G08510.1 (phospholipase C 2 )

HSP 1 Score: 865.9 bits (2236), Expect = 1.9e-251
Identity = 424/599 (70.78%), Postives = 490/599 (81.80%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTY+VCFCFRRRF+ TA EAP EIK +FE+YSENG+M  DHLHRFL++VQK+ K TR
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           +DAQ++I   +      ++ HR  L+L+AFFKYLF D NPPL    VH DM AP+SHY+I
Sbjct: 61  EDAQSIINSAS------SLLHRNGLHLDAFFKYLFGDNNPPLALHKVHHDMDAPISHYFI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           +TGHNSYLTGNQLSSDCS+VPII AL++GVRVIELDIWPNS+KDDIDVLHG TLTTPV L
Sbjct: 121 FTGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPG-SECLKEFP 240
           IKCLK+I+ HAF+ S+YPVV+TLEDHL P+LQ+KVAEMVT  FG+ILF+P   E LKEFP
Sbjct: 181 IKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFP 240

Query: 241 SPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVT 300
           SP SLK+RIIISTKPPKEY       KEG+  +    G+D  +   WGREV S      +
Sbjct: 241 SPNSLKRRIIISTKPPKEY-------KEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKS 300

Query: 301 DYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKV 360
           + K DL  D ND++D +D + KS  +A    P+YK+LIAIHAGKPKGGI  CLKVDP+KV
Sbjct: 301 EAKDDL--DGNDDDDDDDDEDKSKINA---PPQYKHLIAIHAGKPKGGITECLKVDPDKV 360

Query: 361 RRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALN 420
           RRLSLSE+QLEKA   + KQIVRFTQ N+LR+YPKG R  SSNYNP++GW+HGAQMVA N
Sbjct: 361 RRLSLSEEQLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFN 420

Query: 421 MQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGE 480
           MQGYGRSLWLM GMFRANGGCGY+KKPD +LK GSD +IFDPK  L VKTTL+VTVYMGE
Sbjct: 421 MQGYGRSLWLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGE 480

Query: 481 GWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPE 540
           GWY+DF HTHFD YSPPDFYTRVGIAGVP DTVM+KTKTLEDNW+PAW+E FEFPL+VPE
Sbjct: 481 GWYFDFRHTHFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPE 540

Query: 541 LALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           LALLR+EVHEYDMSEKDDFGGQTCLPV EL  GIRA PLH+ KGEKYKSVKLL+  EFV
Sbjct: 541 LALLRLEVHEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEFV 581

BLAST of Lag0040908 vs. TAIR 10
Match: AT3G08510.2 (phospholipase C 2 )

HSP 1 Score: 865.9 bits (2236), Expect = 1.9e-251
Identity = 424/599 (70.78%), Postives = 490/599 (81.80%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTY+VCFCFRRRF+ TA EAP EIK +FE+YSENG+M  DHLHRFL++VQK+ K TR
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           +DAQ++I   +      ++ HR  L+L+AFFKYLF D NPPL    VH DM AP+SHY+I
Sbjct: 61  EDAQSIINSAS------SLLHRNGLHLDAFFKYLFGDNNPPLALHKVHHDMDAPISHYFI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           +TGHNSYLTGNQLSSDCS+VPII AL++GVRVIELDIWPNS+KDDIDVLHG TLTTPV L
Sbjct: 121 FTGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPG-SECLKEFP 240
           IKCLK+I+ HAF+ S+YPVV+TLEDHL P+LQ+KVAEMVT  FG+ILF+P   E LKEFP
Sbjct: 181 IKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFP 240

Query: 241 SPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVT 300
           SP SLK+RIIISTKPPKEY       KEG+  +    G+D  +   WGREV S      +
Sbjct: 241 SPNSLKRRIIISTKPPKEY-------KEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKS 300

Query: 301 DYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKV 360
           + K DL  D ND++D +D + KS  +A    P+YK+LIAIHAGKPKGGI  CLKVDP+KV
Sbjct: 301 EAKDDL--DGNDDDDDDDDEDKSKINA---PPQYKHLIAIHAGKPKGGITECLKVDPDKV 360

Query: 361 RRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALN 420
           RRLSLSE+QLEKA   + KQIVRFTQ N+LR+YPKG R  SSNYNP++GW+HGAQMVA N
Sbjct: 361 RRLSLSEEQLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFN 420

Query: 421 MQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGE 480
           MQGYGRSLWLM GMFRANGGCGY+KKPD +LK GSD +IFDPK  L VKTTL+VTVYMGE
Sbjct: 421 MQGYGRSLWLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTLRVTVYMGE 480

Query: 481 GWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPE 540
           GWY+DF HTHFD YSPPDFYTRVGIAGVP DTVM+KTKTLEDNW+PAW+E FEFPL+VPE
Sbjct: 481 GWYFDFRHTHFDQYSPPDFYTRVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPE 540

Query: 541 LALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           LALLR+EVHEYDMSEKDDFGGQTCLPV EL  GIRA PLH+ KGEKYKSVKLL+  EFV
Sbjct: 541 LALLRLEVHEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEFV 581

BLAST of Lag0040908 vs. TAIR 10
Match: AT3G55940.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 816.2 bits (2107), Expect = 1.7e-236
Identity = 408/603 (67.66%), Postives = 481/603 (79.77%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTY+VCFCFRRR++ T   AP EIK LF+ YS+ G+M  D L RFL++VQK+ K T+
Sbjct: 1   MSKQTYKVCFCFRRRYRHTVSVAPAEIKTLFDNYSDKGLMTTDLLLRFLIDVQKQDKATK 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           ++AQ+++   +      ++ HR  L+L+AFFKYLF+  N PL SL VHQDM APLSHY+I
Sbjct: 61  EEAQDIVNASS------SLLHRNGLHLDAFFKYLFAVTNSPLSSLEVHQDMDAPLSHYFI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           YTGHNSYLTGNQLSSDCS++PII+AL++GVRVIELDIWPNS +D IDVLHGRTLT+PVEL
Sbjct: 121 YTGHNSYLTGNQLSSDCSELPIIEALKKGVRVIELDIWPNSDEDGIDVLHGRTLTSPVEL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPGS-ECLKEFP 240
           IKCL++I+EHAF+ S+YPVV+TLEDHL P LQAKVAEMVT+ FG++LF+P S ECLKEFP
Sbjct: 181 IKCLRAIREHAFDVSDYPVVVTLEDHLTPKLQAKVAEMVTDIFGEMLFTPPSGECLKEFP 240

Query: 241 SPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSL----KG 300
           SP  LKKRI+ISTKPPKEY   K A  +   +K    G D  +   WGREV S     + 
Sbjct: 241 SPAFLKKRIMISTKPPKEY---KAATDDDLVKK----GRDLGDKEVWGREVPSFIRRDRS 300

Query: 301 GTVTDYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVD 360
               D   D D+D +D++D +DGD K   +A    PEYK+LIAI AGKPKGGI  CLKVD
Sbjct: 301 VDKNDSNGDDDDDDDDDDDDDDGDDKIKKNA---PPEYKHLIAIEAGKPKGGITECLKVD 360

Query: 361 PNKVRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQM 420
           P+KVRRLSLSE+QLEKA   + KQIVRFTQ+N+LRVYPKG R  SSNYNP+I W+HGAQM
Sbjct: 361 PDKVRRLSLSEEQLEKASEKYAKQIVRFTQRNLLRVYPKGTRITSSNYNPLIAWSHGAQM 420

Query: 421 VALNMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTV 480
           VA NMQG GRSLW+M GMFR NGGCGY+KKPD +LK    + +FDP+  L VKTTL+VT+
Sbjct: 421 VAFNMQGLGRSLWVMQGMFRGNGGCGYIKKPDLLLK---SNAVFDPEATLPVKTTLRVTI 480

Query: 481 YMGEGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPL 540
           YMGEGWYYDF HTHFD YSPPDFYTRVGIAGVPADTVM+KTKTLEDNW+PAW+E FEFPL
Sbjct: 481 YMGEGWYYDFPHTHFDRYSPPDFYTRVGIAGVPADTVMKKTKTLEDNWIPAWDEVFEFPL 540

Query: 541 SVPELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGF 599
           +VPELALLRIEVHEYDMSEKDDFGGQ CLPV ELR+GIRAVPL N  G K +SVKLL+  
Sbjct: 541 TVPELALLRIEVHEYDMSEKDDFGGQICLPVWELRQGIRAVPLRNQDGVKCRSVKLLVRL 584

BLAST of Lag0040908 vs. TAIR 10
Match: AT3G08510.3 (phospholipase C 2 )

HSP 1 Score: 790.8 bits (2041), Expect = 7.6e-229
Identity = 399/599 (66.61%), Postives = 463/599 (77.30%), Query Frame = 0

Query: 1   MSKQTYRVCFCFRRRFKMTAGEAPDEIKKLFEQYSENGIMNADHLHRFLVEVQKEVKTTR 60
           MSKQTY+VCFCFRRRF+ TA EAP EIK +FE+YSENG+M  DHLHRFL++VQK+ K TR
Sbjct: 1   MSKQTYKVCFCFRRRFRYTASEAPREIKTIFEKYSENGVMTVDHLHRFLIDVQKQDKATR 60

Query: 61  DDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLFSDLNPPLESLGVHQDMSAPLSHYYI 120
           +DAQ++I   +      ++ HR  L+L+AFFKYLF D NPPL    VH DM AP+SHY+I
Sbjct: 61  EDAQSIINSAS------SLLHRNGLHLDAFFKYLFGDNNPPLALHKVHHDMDAPISHYFI 120

Query: 121 YTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDIDVLHGRTLTTPVEL 180
           +TGHNSYLTGNQLSSDCS+VPII AL++GVRVIELDIWPNS+KDDIDVLHG TLTTPV L
Sbjct: 121 FTGHNSYLTGNQLSSDCSEVPIIDALKKGVRVIELDIWPNSNKDDIDVLHGMTLTTPVGL 180

Query: 181 IKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDILFSPG-SECLKEFP 240
           IKCLK+I+ HAF+ S+YPVV+TLEDHL P+LQ+KVAEMVT  FG+ILF+P   E LKEFP
Sbjct: 181 IKCLKAIRAHAFDVSDYPVVVTLEDHLTPDLQSKVAEMVTEIFGEILFTPPVGESLKEFP 240

Query: 241 SPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWGREVSSLKGGTVT 300
           SP SLK+RIIISTKPPKEY       KEG+  +    G+D  +   WGREV S      +
Sbjct: 241 SPNSLKRRIIISTKPPKEY-------KEGKDVEVVQKGKDLGDEEVWGREVPSFIQRNKS 300

Query: 301 DYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKGGIDACLKVDPNKV 360
           + K DL  D ND++D +D + KS  +A    P+YK+LIAIHAGKPKGGI  CLKVDP+KV
Sbjct: 301 EAKDDL--DGNDDDDDDDDEDKSKINA---PPQYKHLIAIHAGKPKGGITECLKVDPDKV 360

Query: 361 RRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPMIGWTHGAQMVALN 420
           RRLSLSE+QLEKA   + KQIVRFTQ N+LR+YPKG R  SSNYNP++GW+HGAQMVA N
Sbjct: 361 RRLSLSEEQLEKAAEKYAKQIVRFTQHNLLRIYPKGTRVTSSNYNPLVGWSHGAQMVAFN 420

Query: 421 MQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLNVKTTLKVTVYMGE 480
           MQGYGRSLWLM GMFRANGGCGY+KKPD +LK GSD +IFDPK  L VKTTL        
Sbjct: 421 MQGYGRSLWLMQGMFRANGGCGYIKKPDLLLKSGSDSDIFDPKATLPVKTTL-------- 480

Query: 481 GWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPAWNEEFEFPLSVPE 540
                                RVGIAGVP DTVM+KTKTLEDNW+PAW+E FEFPL+VPE
Sbjct: 481 ---------------------RVGIAGVPGDTVMKKTKTLEDNWIPAWDEVFEFPLTVPE 540

Query: 541 LALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKYKSVKLLMGFEFV 599
           LALLR+EVHEYDMSEKDDFGGQTCLPV EL  GIRA PLH+ KGEKYKSVKLL+  EFV
Sbjct: 541 LALLRLEVHEYDMSEKDDFGGQTCLPVWELSEGIRAFPLHSRKGEKYKSVKLLVKVEFV 552

BLAST of Lag0040908 vs. TAIR 10
Match: AT2G40116.1 (Phosphoinositide-specific phospholipase C family protein )

HSP 1 Score: 692.6 bits (1786), Expect = 2.8e-199
Identity = 355/611 (58.10%), Postives = 441/611 (72.18%), Query Frame = 0

Query: 6   YRVCFCFRRRFKMTAGEAPDEIKKLFEQYS-----------------ENGIMNADHLHRF 65
           YR+   + R+FK+      D+++  F Q++                   G+M A+ L  F
Sbjct: 19  YRMFKFYNRKFKINEVTPTDDVRDAFCQFAVGGGGGGTDGDSSDGDGSTGVMGAEQLCSF 78

Query: 66  LVEVQKEVKTTRDDAQNMIERCTNELKHLNIFHRKVLNLEAFFKYLF-SDLNPPLESLGV 125
           L +  +   TT  +AQ +I+       H+  F R  L+L+ FF +LF  DLNPP+ +  V
Sbjct: 79  LDDHGE--STTVAEAQRLIDEVIRRRHHVTRFTRHGLDLDDFFNFLFYDDLNPPI-TPHV 138

Query: 126 HQDMSAPLSHYYIYTGHNSYLTGNQLSSDCSDVPIIQALQRGVRVIELDIWPNSSKDDID 185
           HQDM+APLSHY+IYTGHNSYLTGNQLSSDCS+VP+I+ALQRGVRVIELD+WPNS+  DI+
Sbjct: 139 HQDMTAPLSHYFIYTGHNSYLTGNQLSSDCSEVPVIKALQRGVRVIELDLWPNSTGTDIN 198

Query: 186 VLHGRTLTTPVELIKCLKSIKEHAFETSEYPVVITLEDHLPPNLQAKVAEMVTNTFGDIL 245
           VLHGRTLTTPV L+KCLKSI+++AF +S YPV+ITLEDHL P+LQAKVAEM T  FG +L
Sbjct: 199 VLHGRTLTTPVPLMKCLKSIRDYAFSSSPYPVIITLEDHLTPDLQAKVAEMATQIFGQML 258

Query: 246 FSPGSECLKEFPSPESLKKRIIISTKPPKEYLKTKDAPKEGETQKEKTSGEDNKETSAWG 305
           + P S+ L EFPSP SL  RIIISTKPPKEYL++++   + +      S ED    +   
Sbjct: 259 YYPESDSLLEFPSPASLLHRIIISTKPPKEYLESRNPIVKQKDNNVSPSSEDETPRT--- 318

Query: 306 REVSSLKGGTV-TDYKQDLDEDSNDEEDPEDGDTKSTDSAQYIAPEYKNLIAIHAGKPKG 365
            E+ +L+      D++   D D  DEE  ED             P YK LI IHAGKPKG
Sbjct: 319 EEIQTLESMLFDQDFESKSDSDQEDEEASEDQ-----------KPAYKRLITIHAGKPKG 378

Query: 366 GIDACLKVDPNKVRRLSLSEQQLEKAVLTHGKQIVRFTQQNILRVYPKGIRFDSSNYNPM 425
            +   +KV  +KVRRLSLSEQ+L++   ++ + +VRFTQ+N+LR+YPKG RF+SSNY P+
Sbjct: 379 TVKEEMKVVVDKVRRLSLSEQELDRTCSSNSQDVVRFTQRNLLRIYPKGTRFNSSNYKPL 438

Query: 426 IGWTHGAQMVALNMQGYGRSLWLMHGMFRANGGCGYVKKPDFMLKPGSDDEIFDPKVRLN 485
           IGWTHGAQM+A NMQGYG+SLWLMHGMFRANGGCGYVKKP+F++K G  DE+FDP+ +L 
Sbjct: 439 IGWTHGAQMIAFNMQGYGKSLWLMHGMFRANGGCGYVKKPNFLMKKGFHDEVFDPRKKLP 498

Query: 486 VKTTLKVTVYMGEGWYYDFHHTHFDAYSPPDFYTRVGIAGVPADTVMRKTKTLEDNWVPA 545
           VK TLKV VYMG+GW  DF HTHFDAYSPPDFYT++ I GVPAD   +KTK +EDNW P 
Sbjct: 499 VKETLKVKVYMGDGWRMDFSHTHFDAYSPPDFYTKMFIVGVPADNAKKKTKIIEDNWYPI 558

Query: 546 WNEEFEFPLSVPELALLRIEVHEYDMSEKDDFGGQTCLPVAELRRGIRAVPLHNVKGEKY 598
           W+EEF FPL+VPELALLRIEV EYDMSEKDDFGGQTCLPVAELR GIR+VPL++ KGEK 
Sbjct: 559 WDEEFSFPLTVPELALLRIEVREYDMSEKDDFGGQTCLPVAELRPGIRSVPLYDKKGEKM 612

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038892600.10.0e+0094.31phosphoinositide phospholipase C 2-like [Benincasa hispida][more]
KAG6600927.10.0e+0093.65Phosphoinositide phospholipase C 2, partial [Cucurbita argyrosperma subsp. soror... [more]
XP_031741682.10.0e+0093.33phosphoinositide phospholipase C 2 [Cucumis sativus] >KGN50656.1 hypothetical pr... [more]
XP_023534552.10.0e+0093.48phosphoinositide phospholipase C 2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_022956857.10.0e+0093.48phosphoinositide phospholipase C 2-like isoform X2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q390332.6e-25070.78Phosphoinositide phospholipase C 2 OS=Arabidopsis thaliana OX=3702 GN=PLC2 PE=1 ... [more]
Q9LY512.4e-23567.66Phosphoinositide phospholipase C 7 OS=Arabidopsis thaliana OX=3702 GN=PLC7 PE=1 ... [more]
Q8GV434.0e-19858.10Phosphoinositide phospholipase C 6 OS=Arabidopsis thaliana OX=3702 GN=PLC6 PE=2 ... [more]
Q944C12.9e-19657.24Phosphoinositide phospholipase C 4 OS=Arabidopsis thaliana OX=3702 GN=PLC4 PE=2 ... [more]
Q944C26.4e-18053.86Phosphoinositide phospholipase C 5 OS=Arabidopsis thaliana OX=3702 GN=PLC5 PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KSE10.0e+0093.33Phosphoinositide phospholipase C OS=Cucumis sativus OX=3659 GN=Csa_5G203390 PE=4... [more]
A0A6J1GXJ20.0e+0093.48Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111458425 P... [more]
A0A6J1GXM80.0e+0093.32Phosphoinositide phospholipase C OS=Cucurbita moschata OX=3662 GN=LOC111458425 P... [more]
A0A6J1IPZ60.0e+0093.14Phosphoinositide phospholipase C OS=Cucurbita maxima OX=3661 GN=LOC111477133 PE=... [more]
A0A1S3CKB30.0e+0093.12Phosphoinositide phospholipase C OS=Cucumis melo OX=3656 GN=LOC103501442 PE=4 SV... [more]
Match NameE-valueIdentityDescription
AT3G08510.11.9e-25170.78phospholipase C 2 [more]
AT3G08510.21.9e-25170.78phospholipase C 2 [more]
AT3G55940.11.7e-23667.66Phosphoinositide-specific phospholipase C family protein [more]
AT3G08510.37.6e-22966.61phospholipase C 2 [more]
AT2G40116.12.8e-19958.10Phosphoinositide-specific phospholipase C family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 49..73
NoneNo IPR availableGENE3D1.10.238.10coord: 23..103
e-value: 3.4E-12
score: 48.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..327
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 305..319
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 256..285
NoneNo IPR availablePANTHERPTHR10336:SF185PHOSPHOINOSITIDE PHOSPHOLIPASE Ccoord: 2..598
NoneNo IPR availablePROSITEPS50007PIPLC_X_DOMAINcoord: 109..253
score: 41.5242
NoneNo IPR availableCDDcd08599PI-PLCc_plantcoord: 108..437
e-value: 6.06837E-121
score: 355.141
NoneNo IPR availableCDDcd00275C2_PLC_likecoord: 468..597
e-value: 4.39524E-38
score: 134.976
IPR001192Phosphoinositide phospholipase C familyPRINTSPR00390PHPHLIPASECcoord: 388..409
score: 62.09
coord: 564..574
score: 47.51
coord: 409..427
score: 56.41
coord: 114..132
score: 70.11
coord: 140..160
score: 53.1
coord: 237..254
score: 52.83
IPR001192Phosphoinositide phospholipase C familyPANTHERPTHR10336PHOSPHOINOSITIDE-SPECIFIC PHOSPHOLIPASE C FAMILY PROTEINcoord: 2..598
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainSMARTSM00149plcy_3coord: 333..450
e-value: 1.2E-66
score: 237.4
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainPFAMPF00387PI-PLC-Ycoord: 362..448
e-value: 3.8E-29
score: 101.3
IPR001711Phospholipase C, phosphatidylinositol-specific, Y domainPROSITEPS50008PIPLC_Y_DOMAINcoord: 363..449
score: 37.745499
IPR000008C2 domainSMARTSM00239C2_3ccoord: 470..578
e-value: 6.8E-13
score: 58.8
IPR000008C2 domainPFAMPF00168C2coord: 491..571
e-value: 6.4E-11
score: 42.5
IPR000008C2 domainPROSITEPS50004C2coord: 451..580
score: 16.493647
IPR000909Phosphatidylinositol-specific phospholipase C, X domainSMARTSM00148plcx_3coord: 109..253
e-value: 2.1E-72
score: 256.5
IPR000909Phosphatidylinositol-specific phospholipase C, X domainPFAMPF00388PI-PLC-Xcoord: 111..253
e-value: 2.7E-49
score: 166.5
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 463..598
e-value: 2.8E-29
score: 104.0
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 470..597
IPR015359Phosphoinositide-specific phospholipase C, EF-hand-like domainPFAMPF09279EF-hand_likecoord: 25..96
e-value: 9.8E-7
score: 29.2
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilyGENE3D3.20.20.190Phosphatidylinositol (PI) phosphodiesterasecoord: 105..462
e-value: 5.8E-103
score: 346.4
IPR017946PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamilySUPERFAMILY51695PLC-like phosphodiesterasescoord: 109..451
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 11..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0040908.1Lag0040908.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
biological_process GO:0007165 signal transduction
cellular_component GO:0005886 plasma membrane
molecular_function GO:0004435 phosphatidylinositol phospholipase C activity
molecular_function GO:0008081 phosphoric diester hydrolase activity