Lag0039769 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039769
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionprotein NRT1/ PTR FAMILY 5.10-like
Locationchr2: 49589895 .. 49591821 (-)
RNA-Seq ExpressionLag0039769
SyntenyLag0039769
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGTTCCTCTGTTGGAGGAGACGGTGGAGGGCGCAGTCGACTACAGTGGCCGGCCAGTCCGCAGATCCAACTCCGGCGGCTGGAGATCCGCCTCACTCATCATAGGTAAATCTAAATACCATCAATCGCTTCATCTCTTTCTTCTGTTTCGCCATCCCTCTCCAATTGTGATTAGGAGTGGAGGCTGCCGAGAGATTCGCCTATTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCATCAGTCCACGGCCACTGCAGCGGAGAACGTCAATGCCTGGTCCGGCGCCGCCTCCTTGCTACCTCTTCTCGGCGCCTTTCTCGCCGATTCCTTCCTTGGACGATACCGCACTATTGCTCTTTCCTCCGCCCTCTACATCCTGGTTCCTCCACTACTCTATTCTATTCTCCTTTAATTAGGGCTCTGCTAATTAGGGCTTGGATATTTTCACAATATAGTTTTCTTTTGGAATTCAGGGGCTTGGGTTGTTGACTGTGTCTGCAACATTACCTTCTCCCAGCACTTCTGCCTGCCAACAGACGGAAAAATCCGTAGCATGTTCCCCCACTCTTGTCCAACTAGTTTTATTTTTCTTCTCCTTATATCTGGTGGCTTTTGCTCAAGGCGGACACAAGCCTTGCGTCCAAGCTTTCGGAGCCGATCAATTTGATGGACAACATCCAGAAGAGAACAAAGCTAAAAGCTCTTTCTTTAATTGGTGGTACTTTGGTCTCTCTCTGGCCATTCTGGCATCTGTTAATATCTTGAACTATGTGCAAGATAACCTTAGCTGGAGTCTTGGATTTGGAATTTCTTGTACTGCTATGGTCTTTGCATTCCTAGTCTTCTTGCTTGGAACAAGGACGTACAGGTACAGTAACAGAAGAGATGAGAAAAACCCATTGGTGAGGATTGGACGGGTGTTTATCACGGCAGTAAGAAATTGGCATGTAAAATCTTCAGAAATTGCTCACGAAGAGGAAATCCGTGGCCTTTTACCTCACCATAGCTCTCAACAATTAAGGTAACACTCATTATATGGATATAAAACATCATAATAATCCTTTTCAGCTATTGGATCCTCTAATTTGGCAGGTTCCTGGACAAAGCTTTAGTTGCACCTAATGGTTCAAAGGAAGACGGTCACGCATGTAGTGTCGGCGAAGTTGAGGAAGCAAAAGCAGTACTTAGGCTTGCTCCCATATGGGTTACCTGCTTAGCATATGCTATTGTATTCTCTCAGTTTTCTACCTTCTTTACAAAACAAGGAGTCTCAATGGACAGATCAATAGTTCCTGGATTTGAAATACCAGCTGCTTGGCTTCAATCCTTTATCAGTCTGTCCATTGTCATTTGCCTCCCGACATATGATCGTATACTCATACCGATTGCGAGAAATTTCACTGGAAAACCTTCTGGAATTACAATGCTACAGAGAATTGGATTTGGGATGCTAGTATCTGCCCTTTCCATGATCGTTGCTGCAGTGGTCGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCCAAGGCGACTGTTCCTCTGAGTATATGGTGGCTGGTTCCTCAATATGTACTGTTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGATCAGGTTCCAAGTGGATTAAGAAGCATGGGTCTCTCCCTGTACCTGAGTATTTTTGGCGTTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAGGTTAACCAGTGGGGATGGCAGAGAAAGCTGGTTCGACAATAATCTCAACAAAGCTCATCTTGATTACTTCTACTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTTCTCTGTGCTGCAAGAACTTACATCTACAATAAGGGGAACACCACATGA

mRNA sequence

ATGGAGGTTCCTCTGTTGGAGGAGACGGTGGAGGGCGCAGTCGACTACAGTGGCCGGCCAGTCCGCAGATCCAACTCCGGCGGCTGGAGATCCGCCTCACTCATCATAGGAGTGGAGGCTGCCGAGAGATTCGCCTATTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCATCAGTCCACGGCCACTGCAGCGGAGAACGTCAATGCCTGGTCCGGCGCCGCCTCCTTGCTACCTCTTCTCGGCGCCTTTCTCGCCGATTCCTTCCTTGGACGATACCGCACTATTGCTCTTTCCTCCGCCCTCTACATCCTGGGGCTTGGGTTGTTGACTGTGTCTGCAACATTACCTTCTCCCAGCACTTCTGCCTGCCAACAGACGGAAAAATCCGTAGCATGTTCCCCCACTCTTGTCCAACTAGTTTTATTTTTCTTCTCCTTATATCTGGTGGCTTTTGCTCAAGGCGGACACAAGCCTTGCGTCCAAGCTTTCGGAGCCGATCAATTTGATGGACAACATCCAGAAGAGAACAAAGCTAAAAGCTCTTTCTTTAATTGGTGGTACTTTGGTCTCTCTCTGGCCATTCTGGCATCTGTTAATATCTTGAACTATGTGCAAGATAACCTTAGCTGGAGTCTTGGATTTGGAATTTCTTGTACTGCTATGGTCTTTGCATTCCTAGTCTTCTTGCTTGGAACAAGGACGTACAGGTACAGTAACAGAAGAGATGAGAAAAACCCATTGGTGAGGATTGGACGGGTGTTTATCACGGCAGTAAGAAATTGGCATGTAAAATCTTCAGAAATTGCTCACGAAGAGGAAATCCGTGGCCTTTTACCTCACCATAGCTCTCAACAATTAAGGTTCCTGGACAAAGCTTTAGTTGCACCTAATGGTTCAAAGGAAGACGGTCACGCATGTAGTGTCGGCGAAGTTGAGGAAGCAAAAGCAGTACTTAGGCTTGCTCCCATATGGGTTACCTGCTTAGCATATGCTATTGTATTCTCTCAGTTTTCTACCTTCTTTACAAAACAAGGAGTCTCAATGGACAGATCAATAGTTCCTGGATTTGAAATACCAGCTGCTTGGCTTCAATCCTTTATCAGTCTGTCCATTGTCATTTGCCTCCCGACATATGATCGTATACTCATACCGATTGCGAGAAATTTCACTGGAAAACCTTCTGGAATTACAATGCTACAGAGAATTGGATTTGGGATGCTAGTATCTGCCCTTTCCATGATCGTTGCTGCAGTGGTCGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCCAAGGCGACTGTTCCTCTGAGTATATGGTGGCTGGTTCCTCAATATGTACTGTTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGATCAGGTTCCAAGTGGATTAAGAAGCATGGGTCTCTCCCTGTACCTGAGTATTTTTGGCGTTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAGGTTAACCAGTGGGGATGGCAGAGAAAGCTGGTTCGACAATAATCTCAACAAAGCTCATCTTGATTACTTCTACTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTTCTCTGTGCTGCAAGAACTTACATCTACAATAAGGGGAACACCACATGA

Coding sequence (CDS)

ATGGAGGTTCCTCTGTTGGAGGAGACGGTGGAGGGCGCAGTCGACTACAGTGGCCGGCCAGTCCGCAGATCCAACTCCGGCGGCTGGAGATCCGCCTCACTCATCATAGGAGTGGAGGCTGCCGAGAGATTCGCCTATTACGGAGTTTCCTCCAACTTGATTAACTTCTTGACGGACCAGCTCCATCAGTCCACGGCCACTGCAGCGGAGAACGTCAATGCCTGGTCCGGCGCCGCCTCCTTGCTACCTCTTCTCGGCGCCTTTCTCGCCGATTCCTTCCTTGGACGATACCGCACTATTGCTCTTTCCTCCGCCCTCTACATCCTGGGGCTTGGGTTGTTGACTGTGTCTGCAACATTACCTTCTCCCAGCACTTCTGCCTGCCAACAGACGGAAAAATCCGTAGCATGTTCCCCCACTCTTGTCCAACTAGTTTTATTTTTCTTCTCCTTATATCTGGTGGCTTTTGCTCAAGGCGGACACAAGCCTTGCGTCCAAGCTTTCGGAGCCGATCAATTTGATGGACAACATCCAGAAGAGAACAAAGCTAAAAGCTCTTTCTTTAATTGGTGGTACTTTGGTCTCTCTCTGGCCATTCTGGCATCTGTTAATATCTTGAACTATGTGCAAGATAACCTTAGCTGGAGTCTTGGATTTGGAATTTCTTGTACTGCTATGGTCTTTGCATTCCTAGTCTTCTTGCTTGGAACAAGGACGTACAGGTACAGTAACAGAAGAGATGAGAAAAACCCATTGGTGAGGATTGGACGGGTGTTTATCACGGCAGTAAGAAATTGGCATGTAAAATCTTCAGAAATTGCTCACGAAGAGGAAATCCGTGGCCTTTTACCTCACCATAGCTCTCAACAATTAAGGTTCCTGGACAAAGCTTTAGTTGCACCTAATGGTTCAAAGGAAGACGGTCACGCATGTAGTGTCGGCGAAGTTGAGGAAGCAAAAGCAGTACTTAGGCTTGCTCCCATATGGGTTACCTGCTTAGCATATGCTATTGTATTCTCTCAGTTTTCTACCTTCTTTACAAAACAAGGAGTCTCAATGGACAGATCAATAGTTCCTGGATTTGAAATACCAGCTGCTTGGCTTCAATCCTTTATCAGTCTGTCCATTGTCATTTGCCTCCCGACATATGATCGTATACTCATACCGATTGCGAGAAATTTCACTGGAAAACCTTCTGGAATTACAATGCTACAGAGAATTGGATTTGGGATGCTAGTATCTGCCCTTTCCATGATCGTTGCTGCAGTGGTCGAGATAAAAAGGCTCAAAACAGCTCAAGAATATGGTTTGGTTGATCTGCCCAAGGCGACTGTTCCTCTGAGTATATGGTGGCTGGTTCCTCAATATGTACTGTTTGGAGTTGCTGATGTTTTCACCATGGTTGGCTTGCAGGAGTTTTTCTATGATCAGGTTCCAAGTGGATTAAGAAGCATGGGTCTCTCCCTGTACCTGAGTATTTTTGGCGTTGGCAACTTTTTAAGCAGCTTTCTCATCTCTGCCATTGAAAGGTTAACCAGTGGGGATGGCAGAGAAAGCTGGTTCGACAATAATCTCAACAAAGCTCATCTTGATTACTTCTACTGGTTGCTTGCTGGGCTTAGTGCTATTGGGTTGGCTGCTTTTCTCTGTGCTGCAAGAACTTACATCTACAATAAGGGGAACACCACATGA

Protein sequence

MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQLHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVAPNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPGFEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIVAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSAIGLAAFLCAARTYIYNKGNTT
Homology
BLAST of Lag0039769 vs. NCBI nr
Match: XP_023535812.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 975.3 bits (2520), Expect = 2.2e-280
Identity = 486/563 (86.32%), Postives = 525/563 (93.25%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S+CQQTEKS+ CSP L Q++LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW + SSEIAHEEEIRGLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           FE+PAA LQS I LSIV+ LP YDRILIPIARNFTGKPSGITMLQRIGFGML+SALSM++
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SA+GLAAFLC ARTYIY KGNTT
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNTT 563

BLAST of Lag0039769 vs. NCBI nr
Match: XP_023535811.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 974.5 bits (2518), Expect = 3.8e-280
Identity = 485/563 (86.15%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVN WSG ASLLPLLGAFLADSF+GRYRTIALSS LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSGVASLLPLLGAFLADSFIGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S+CQQTEKS+ CSP L Q++LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW + SSEIAHEEEIRGLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRINSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PNSSKEGGHTCTIREVEEAKAVLRLVPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           FE+PAA LQS I LSIV+ LP YDRILIPIARNFTGKPSGITMLQRIGFGML+SALSM++
Sbjct: 361 FEVPAASLQSVIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SA+GLAAFLC ARTYIY KGNTT
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNTT 563

BLAST of Lag0039769 vs. NCBI nr
Match: XP_022973964.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima])

HSP 1 Score: 972.2 bits (2512), Expect = 1.9e-279
Identity = 487/563 (86.50%), Postives = 524/563 (93.07%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SP  S CQQTEKS+ CSP L Q++LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW V SSEIAHEEEIRGLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN SKE GH CS+ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           F++PAA LQSFISLSIV+ L  YDRILIPIARNFTGKPSGITMLQRIGFGML+SALSM++
Sbjct: 361 FDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF++NLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SAIGLAAFLC ARTYIY+KGNTT
Sbjct: 541 SAIGLAAFLCFARTYIYSKGNTT 563

BLAST of Lag0039769 vs. NCBI nr
Match: XP_023535809.1 (protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023535810.1 protein NRT1/ PTR FAMILY 5.10-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023535814.1 protein NRT1/ PTR FAMILY 5.10-like isoform X6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 971.5 bits (2510), Expect = 3.2e-279
Identity = 485/563 (86.15%), Postives = 524/563 (93.07%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S+CQQTEKS+ CSP L Q++LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPSISSCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW V SSEIAHEEE RGLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEETRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYA+VFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLLPIWVTCLAYAVVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           F++PAA LQSFI LSIV+ LP YDRILIPIARNFTGKPSGITMLQRIGFGML+S+LSM++
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSSLSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SAIGLAAFLC ARTYIY+K NTT
Sbjct: 541 SAIGLAAFLCFARTYIYSKKNTT 563

BLAST of Lag0039769 vs. NCBI nr
Match: XP_022925410.1 (protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata])

HSP 1 Score: 971.5 bits (2510), Expect = 3.2e-279
Identity = 487/563 (86.50%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVE AVDY+GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQTEKS+ CSP L Q++LFFFSLY+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW V SSEIAHEEEIRGLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALLV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           F++PAA LQSFISLSIV+ LP YDRILIPIARNFTGKPSGITMLQRIGFGML+SALSM++
Sbjct: 361 FDVPAASLQSFISLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SAIGLAAFLC ARTYIY+K NTT
Sbjct: 541 SAIGLAAFLCFARTYIYSKKNTT 563

BLAST of Lag0039769 vs. ExPASy Swiss-Prot
Match: Q0WP01 (Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV=1)

HSP 1 Score: 726.5 bits (1874), Expect = 2.3e-208
Identity = 370/560 (66.07%), Postives = 448/560 (80.00%), Query Frame = 0

Query: 4   PLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQLHQ 63
           PLL  T    VDY  +P  +S+SGGWRSA  IIGVE AERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSP 123
           STA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +SALYI+GLG+LT+SA +PS 
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSD 132

Query: 124 STSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKA 183
               C+ +    +CSP   Q++ FF +LYLVA AQGGHKPCVQAFGADQFD + PEE KA
Sbjct: 133 ----CKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKA 192

Query: 184 KSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYS 243
           KSSFFNWWYFG+    L ++ +LNY+QDNLSW+LGFGI C AMV A +V LLGT TYR+S
Sbjct: 193 KSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFS 252

Query: 244 NRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVAPNG 303
            RR++++P VRIG V++ AV+NW V + ++A  EE  GL+   SSQQ  FL+KALVA NG
Sbjct: 253 IRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNG 312

Query: 304 SKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPGFEI 363
           S      CS+ E+EEAK+VLRLAPIW+TCL YA+VF+Q  TFFTKQG +M+RSI PG++I
Sbjct: 313 S------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKI 372

Query: 364 PAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIVAAV 423
             A LQSFISLSIVI +P YDR+LIPIAR+FT KP GITMLQRIG G+ +S L+M+VAA+
Sbjct: 373 SPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAAL 432

Query: 424 VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 483
           VE+KRLKTA +YGLVD P ATVP+S+WWLVPQYVLFG+ DVF MVGLQEFFYDQVP+ LR
Sbjct: 433 VEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELR 492

Query: 484 SMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 543
           S+GL+LYLSIFG+GNFLSSF+IS IE+ TS  G+ SWF NNLN+AHLDYFYWLLA LS I
Sbjct: 493 SVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFI 552

Query: 544 GLAAFLCAARTYIYNKGNTT 564
           GLA++L  A++Y+  + +T+
Sbjct: 553 GLASYLYVAKSYVSKRLDTS 557

BLAST of Lag0039769 vs. ExPASy Swiss-Prot
Match: Q8VZE2 (Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV=2)

HSP 1 Score: 616.7 bits (1589), Expect = 2.6e-175
Identity = 310/560 (55.36%), Postives = 409/560 (73.04%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQL 61
           E+ L EE V  AVD+ G   RRSN+G WR+A  IIGVE AERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            +STA AA NVNAWSG A+LLP+LGAF+AD+FLGRYRTI +SS +Y+LGL  LT+SA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLI 125

Query: 122 SPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
             +T     T   +         VLFFFSLYLVA  Q GHKPCVQAFGADQFD +  +E 
Sbjct: 126 PNTTEVTSSTSSFLN--------VLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 182 KAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
             +SSFFNWWY  LS  I  ++ ++ Y+Q+  SW+ GFGI C  MV + ++F+ G R YR
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 242 YSNRRDEK--NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALV 301
           YS RR E+  NP  RIGRVF  A++N  + SS++   E    L  + S ++  F +KAL+
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALL 305

Query: 302 APNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVP 361
            PN S +  +A    +VE+A A++RL P+W T LAYAI ++Q+ TFFTKQGV+MDR+I+P
Sbjct: 306 VPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILP 365

Query: 362 GFEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMI 421
           G +IP A LQ FI +SIV+ +P YDR+ +PIAR  T +P GIT L+RIG G+++S ++M+
Sbjct: 366 GVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMV 425

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           +AA+VE KRL+TA+E+GL+D P+AT+P+SIWWL+PQY+L G+ADV+T+VG+QEFFY QVP
Sbjct: 426 IAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVP 485

Query: 482 SGLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRS+GL+LYLS  GVG+ LSS LIS I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAI 545

Query: 542 LSAIGLAAFLCAARTYIYNK 560
           +SA+G   FL  +++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 553

BLAST of Lag0039769 vs. ExPASy Swiss-Prot
Match: Q0WSZ6 (Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV=2)

HSP 1 Score: 615.1 bits (1585), Expect = 7.6e-175
Identity = 312/557 (56.01%), Postives = 407/557 (73.07%), Query Frame = 0

Query: 5   LLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQLHQS 64
           L EE V  AVD+ G   RRS +G WR+A  IIGVE AERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPS 124
           TA AA NVNAWSG +++LPLLGAF+AD+FLGRY TI ++S +Y+LGL  LT+SA L   +
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNN 128

Query: 125 TSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAK 184
           T         V  SP+     LFFFSLYLVA  Q GHKPCVQAFGADQFD ++P+EN  +
Sbjct: 129 T--------EVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDR 188

Query: 185 SSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSN 244
           SSFFNWWY  +   I  ++ ++ Y+Q+N+SW+LGFGI C  MV + ++F+LG ++YR+S 
Sbjct: 189 SSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSK 248

Query: 245 RR--DEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVAPN 304
            R  +E NP  RIGRVF  A +N  + SS++   E I       S ++L FL+KAL+ PN
Sbjct: 249 TRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPN 308

Query: 305 GSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPGFE 364
            S E   AC   +VE+A A++RL P+W+T LAYAI F+Q+ TFFTKQGV+M+R+I PG E
Sbjct: 309 DSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVE 368

Query: 365 IPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIVAA 424
           IP A LQ  IS+SIV+ +P YDR+L+PI R+ T  P GIT L+RIG GM+++ L+M+VAA
Sbjct: 369 IPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAA 428

Query: 425 VVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 484
           +VE KRL+TA+EYGL+D PK T+P+SIWWL PQY+L G+ADV T+VG+QEFFY QVP+ L
Sbjct: 429 LVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTEL 488

Query: 485 RSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSA 544
           RS+GL++YLS  GVG+ LSS LI  I+  T GD   SWF++NLN+AHLDYFYWLLA +SA
Sbjct: 489 RSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSA 548

Query: 545 IGLAAFLCAARTYIYNK 560
           +G   FL  +++YIY +
Sbjct: 549 VGFFTFLFISKSYIYRR 557

BLAST of Lag0039769 vs. ExPASy Swiss-Prot
Match: Q9SK96 (Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV=1)

HSP 1 Score: 607.4 bits (1565), Expect = 1.6e-172
Identity = 308/560 (55.00%), Postives = 405/560 (72.32%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQL 61
           E  L+E++V  +VD+ G P  +S++GGWRSA  IIGVE  ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S +YILGLGLLT+S+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 122 SPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
               S  +Q  ++ +  P     +LFF SLYLVA  QGGHKPCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 182 KAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
            ++ SFFNWW+  LS  I  S+ ++ YVQDN++W+LGFGI C  MV A  +FL G +TYR
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 242 Y--SNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALV 301
           Y   +R  + N   RIGRVF+ A +N  +K   + H  ++          QL FL KAL+
Sbjct: 247 YPRGDREGKNNAFARIGRVFLVAFKNRKLK---LTHSGQLEVGSYKKCKGQLEFLAKALL 306

Query: 302 APNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVP 361
              G  E    CS  +VE+A A++RL PIW+T +   I ++Q++TFFTKQGV++DR I+P
Sbjct: 307 PGEGGVE---PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILP 366

Query: 362 GFEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMI 421
           GFEIP A  Q+ I LSI I +PTY+R+ +P+AR  T KPSGITMLQRIG GM++S+L+M+
Sbjct: 367 GFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMV 426

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           VAA+VE+KRL+TA+E+GLVD P AT+P+SIWW VPQY+L G+ DVF++VG QEFFYDQVP
Sbjct: 427 VAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVP 486

Query: 482 SGLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRS+GL+L LS  G+ +FLS FLI+ I   T  +G +SWF+ NLN+AH+DYFYWLLA 
Sbjct: 487 TELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAA 546

Query: 542 LSAIGLAAFLCAARTYIYNK 560
            +AIG  AFL  +R Y+Y +
Sbjct: 547 FTAIGFLAFLLLSRLYVYRR 560

BLAST of Lag0039769 vs. ExPASy Swiss-Prot
Match: Q9C7U1 (Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 3.7e-169
Identity = 306/557 (54.94%), Postives = 406/557 (72.89%), Query Frame = 0

Query: 8   ETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQLHQSTAT 67
           E VE +VD+ G P  RS+SG W+S+   +  E AE+FAY+G++SNLI + T+ L +STA 
Sbjct: 17  ENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAV 76

Query: 68  AAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPSTSA 127
           AA NVN W G A+ LPL+   +ADSFLGR+RTI L+S+ YI+GLGLLT SAT+P    S 
Sbjct: 77  AASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SL 136

Query: 128 CQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAKSSF 187
           C   E   +C  + V++++FF +LYL+A  +GG K C++AFGADQFD Q P E+KAKSS+
Sbjct: 137 CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSY 196

Query: 188 FNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYS---- 247
           FNW YF +S+ IL +  + NYVQ+NLSW+LG+ I C +M+ A  +FLLG +TYR+S    
Sbjct: 197 FNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGE 256

Query: 248 NRRDEK--NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVAP 307
            R+ +K  NP VRIGRVF+ A RN     S+         LLP+ S+++ RFLD+A++  
Sbjct: 257 GRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCL------LLPNESTKKFRFLDRAVI-- 316

Query: 308 NGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPGF 367
                   +C   EVEEAKAVL L PIW+  L + IVF+Q  TFFTKQG +MDRSI    
Sbjct: 317 --------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTL 376

Query: 368 EIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIVA 427
           ++PAA LQ FISL+I++ +P YDR+ +PIAR+ T KP+GIT LQRI  G+ +S +SM++A
Sbjct: 377 QVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIA 436

Query: 428 AVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSG 487
           A+VE+KRLKTA+++GLVD PKATVP+S+ WL+PQY+LFGV+DVFTMVGLQEFFY +VP  
Sbjct: 437 ALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQ 496

Query: 488 LRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLS 547
           LRSMGL+LYLSI G+GNFLSSF++S IE  TS  G+ SWF NNLN+AHLDYFYWLLA LS
Sbjct: 497 LRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLS 552

Query: 548 AIGLAAFLCAARTYIYN 559
           ++     +  A++Y+YN
Sbjct: 557 SLAFIFTVYFAKSYLYN 552

BLAST of Lag0039769 vs. ExPASy TrEMBL
Match: A0A6J1IG54 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 PE=3 SV=1)

HSP 1 Score: 972.2 bits (2512), Expect = 9.1e-280
Identity = 487/563 (86.50%), Postives = 524/563 (93.07%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY+GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SP  S CQQTEKS+ CSP L Q++LFF SLY+VAFAQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 HSPRISGCQQTEKSLPCSPGLFQVILFFSSLYVVAFAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW V SSEIAHEEEIRGLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN SKE GH CS+ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PNSSKEGGHTCSISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           F++PAA LQSFISLSIV+ L  YDRILIPIARNFTGKPSGITMLQRIGFGML+SALSM++
Sbjct: 361 FDVPAASLQSFISLSIVVSLLIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE+L+SGDG+ESWF++NLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIEKLSSGDGKESWFNSNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SAIGLAAFLC ARTYIY+KGNTT
Sbjct: 541 SAIGLAAFLCFARTYIYSKGNTT 563

BLAST of Lag0039769 vs. ExPASy TrEMBL
Match: A0A6J1EHW2 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710 PE=3 SV=1)

HSP 1 Score: 971.5 bits (2510), Expect = 1.5e-279
Identity = 487/563 (86.50%), Postives = 523/563 (92.90%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVE AVDY+GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLI+FLTDQ
Sbjct: 1   MEAPLLEDTVECAVDYNGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLISFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALS+ LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSTVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQTEKS+ CSP L Q++LFFFSLY+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFFSLYVVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW V SSEIAHEEEIRGLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEIRGLLPHHSSQQFRFLNKALLV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN SKE GH C++ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PNSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           F++PAA LQSFISLSIV+ LP YDRILIPIARNFTGKPSGITMLQRIGFGML+SALSM++
Sbjct: 361 FDVPAASLQSFISLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SAIGLAAFLC ARTYIY+K NTT
Sbjct: 541 SAIGLAAFLCFARTYIYSKKNTT 563

BLAST of Lag0039769 vs. ExPASy TrEMBL
Match: A0A6J1EC56 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731 PE=3 SV=1)

HSP 1 Score: 964.9 bits (2493), Expect = 1.4e-277
Identity = 483/563 (85.79%), Postives = 517/563 (91.83%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           ME PLLE+TVEGAVDY GRPVRRS+SGGWRSA+LIIGVE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEAPLLEDTVEGAVDYHGRPVRRSDSGGWRSAALIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QSTATAAENVN WS AASLLPLLGAFLADSFLGRYRTIALSS LYILGLGLLTVSATL
Sbjct: 61  LGQSTATAAENVNVWSAAASLLPLLGAFLADSFLGRYRTIALSSVLYILGLGLLTVSATL 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPS S CQQTEKS+ CSP L Q++LFF S+Y+VAFAQGGHKPCVQAFGADQFD QHPEE
Sbjct: 121 HSPSISGCQQTEKSLPCSPGLFQVILFFSSVYIVAFAQGGHKPCVQAFGADQFDAQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           +KAKSSFFNWWYF +SLA  A+VNILNYVQDNLSWSLGFGI C +MV A +VFLLGTRTY
Sbjct: 181 SKAKSSFFNWWYFCISLATFATVNILNYVQDNLSWSLGFGIPCISMVLALVVFLLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+ NR DE+NP VRIGRVFITAVRNW V SSEIAHEEEI GLLPHHSSQQ RFL+KAL+ 
Sbjct: 241 RFINRGDEENPFVRIGRVFITAVRNWRVNSSEIAHEEEICGLLPHHSSQQFRFLNKALIV 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           P  SKE GH C++ EVEEAKAVLRL PIWVTCLAYAIVFSQ STFFTKQGV+M+RSIVPG
Sbjct: 301 PYSSKEGGHTCTISEVEEAKAVLRLVPIWVTCLAYAIVFSQSSTFFTKQGVTMNRSIVPG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           F++PAA LQSFI LSIV+ LP YDRILIPIARNFTGKPSGITMLQRIGFGML+SALSM++
Sbjct: 361 FDVPAASLQSFIGLSIVVSLPIYDRILIPIARNFTGKPSGITMLQRIGFGMLLSALSMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRL+TAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQ PS
Sbjct: 421 AALVEIKRLETAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQAPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFGVGNFLSSFLISAIE L+SGDG+ESWF+NNLNKAHLDYFYWLLAGL
Sbjct: 481 GLRSIGLSLYLSIFGVGNFLSSFLISAIENLSSGDGKESWFNNNLNKAHLDYFYWLLAGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNTT 564
           SA+GLAAFLC ARTYIY KGNTT
Sbjct: 541 SAVGLAAFLCFARTYIYGKGNTT 563

BLAST of Lag0039769 vs. ExPASy TrEMBL
Match: A0A1S3ATI3 (protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 6.1e-276
Identity = 480/562 (85.41%), Postives = 517/562 (91.99%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           MEVPLL+ETVEGAVDY+G PV RSNSGGWRSASLIIGVE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LSSALYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPSTSACQQTEKS+ CSP L Q++LFFFSLYLV  AQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           + +KSSFFNWWYFG+SLA  A+VNILNYVQDNLSWSLGFGI C AMV A +VF LGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+SNR DE+NP VRIGRVFITAV+NW V SSE+A EEEIRGLLPHHSS+QLRFLDKAL+A
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN  KEDG ACS+ EVEEAKAVLRL PIWVTCLA+AIVFSQ STFFTKQGV+MDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           FE+PAA LQSFISL+IVI L  YDRILIPIARNFTGKPSGIT LQRIGFGML+SA+SM++
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFG+GNFLSSFLISAIE+LTSG+G++SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNT 563
           SA+GLAAFL + RTYIYNKGNT
Sbjct: 541 SALGLAAFLFSTRTYIYNKGNT 562

BLAST of Lag0039769 vs. ExPASy TrEMBL
Match: A0A5A7TJ76 (Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1167G00110 PE=3 SV=1)

HSP 1 Score: 959.5 bits (2479), Expect = 6.1e-276
Identity = 480/562 (85.41%), Postives = 517/562 (91.99%), Query Frame = 0

Query: 1   MEVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQ 60
           MEVPLL+ETVEGAVDY+G PV RSNSGGWRSASLIIGVE AERFAYYGVSSNLINFLTDQ
Sbjct: 1   MEVPLLDETVEGAVDYNGNPVCRSNSGGWRSASLIIGVEVAERFAYYGVSSNLINFLTDQ 60

Query: 61  LHQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATL 120
           L QST TAA+NVNAWSG ASLLPLLGAFLADSFLGRY TI LSSALYILGLGLLT+SA +
Sbjct: 61  LQQSTVTAAKNVNAWSGTASLLPLLGAFLADSFLGRYWTIVLSSALYILGLGLLTMSALI 120

Query: 121 PSPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEE 180
            SPSTSACQQTEKS+ CSP L Q++LFFFSLYLV  AQGGHKPCVQAFGADQFDGQHPEE
Sbjct: 121 SSPSTSACQQTEKSLPCSPNLFQVILFFFSLYLVGLAQGGHKPCVQAFGADQFDGQHPEE 180

Query: 181 NKAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTY 240
           + +KSSFFNWWYFG+SLA  A+VNILNYVQDNLSWSLGFGI C AMV A +VF LGTRTY
Sbjct: 181 SISKSSFFNWWYFGISLATFATVNILNYVQDNLSWSLGFGIPCIAMVLALVVFFLGTRTY 240

Query: 241 RYSNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVA 300
           R+SNR DE+NP VRIGRVFITAV+NW V SSE+A EEEIRGLLPHHSS+QLRFLDKAL+A
Sbjct: 241 RFSNRGDEENPFVRIGRVFITAVKNWRVNSSEMAREEEIRGLLPHHSSKQLRFLDKALIA 300

Query: 301 PNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPG 360
           PN  KEDG ACS+ EVEEAKAVLRL PIWVTCLA+AIVFSQ STFFTKQGV+MDRSI  G
Sbjct: 301 PNSLKEDGRACSISEVEEAKAVLRLVPIWVTCLAFAIVFSQSSTFFTKQGVTMDRSIAFG 360

Query: 361 FEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIV 420
           FE+PAA LQSFISL+IVI L  YDRILIPIARNFTGKPSGIT LQRIGFGML+SA+SM++
Sbjct: 361 FEVPAASLQSFISLAIVISLMIYDRILIPIARNFTGKPSGITTLQRIGFGMLLSAISMVI 420

Query: 421 AAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPS 480
           AA+VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVAD FTMVGLQEFFYDQVPS
Sbjct: 421 AALVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADAFTMVGLQEFFYDQVPS 480

Query: 481 GLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGL 540
           GLRS+GLSLYLSIFG+GNFLSSFLISAIE+LTSG+G++SWFDNNLNKAHLDYFYWLL GL
Sbjct: 481 GLRSIGLSLYLSIFGIGNFLSSFLISAIEKLTSGNGKQSWFDNNLNKAHLDYFYWLLVGL 540

Query: 541 SAIGLAAFLCAARTYIYNKGNT 563
           SA+GLAAFL + RTYIYNKGNT
Sbjct: 541 SALGLAAFLFSTRTYIYNKGNT 562

BLAST of Lag0039769 vs. TAIR 10
Match: AT1G22540.1 (Major facilitator superfamily protein )

HSP 1 Score: 726.5 bits (1874), Expect = 1.7e-209
Identity = 370/560 (66.07%), Postives = 448/560 (80.00%), Query Frame = 0

Query: 4   PLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQLHQ 63
           PLL  T    VDY  +P  +S+SGGWRSA  IIGVE AERFAYYG+SSNLI +LT  L Q
Sbjct: 13  PLLAVT----VDYRNKPAVKSSSGGWRSAGFIIGVEVAERFAYYGISSNLITYLTGPLGQ 72

Query: 64  STATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSP 123
           STA AA NVNAWSG ASLLPLLGAF+ADSFLGR+RTI  +SALYI+GLG+LT+SA +PS 
Sbjct: 73  STAAAAANVNAWSGTASLLPLLGAFVADSFLGRFRTILAASALYIVGLGVLTLSAMIPSD 132

Query: 124 STSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKA 183
               C+ +    +CSP   Q++ FF +LYLVA AQGGHKPCVQAFGADQFD + PEE KA
Sbjct: 133 ----CKVSNLLSSCSPRF-QVITFFSALYLVALAQGGHKPCVQAFGADQFDEKEPEECKA 192

Query: 184 KSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYS 243
           KSSFFNWWYFG+    L ++ +LNY+QDNLSW+LGFGI C AMV A +V LLGT TYR+S
Sbjct: 193 KSSFFNWWYFGMCFGTLTTLWVLNYIQDNLSWALGFGIPCIAMVVALVVLLLGTCTYRFS 252

Query: 244 NRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVAPNG 303
            RR++++P VRIG V++ AV+NW V + ++A  EE  GL+   SSQQ  FL+KALVA NG
Sbjct: 253 IRREDQSPFVRIGNVYVAAVKNWSVSALDVAAAEERLGLVSCSSSQQFSFLNKALVAKNG 312

Query: 304 SKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPGFEI 363
           S      CS+ E+EEAK+VLRLAPIW+TCL YA+VF+Q  TFFTKQG +M+RSI PG++I
Sbjct: 313 S------CSIDELEEAKSVLRLAPIWLTCLVYAVVFAQSPTFFTKQGATMERSITPGYKI 372

Query: 364 PAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIVAAV 423
             A LQSFISLSIVI +P YDR+LIPIAR+FT KP GITMLQRIG G+ +S L+M+VAA+
Sbjct: 373 SPATLQSFISLSIVIFIPIYDRVLIPIARSFTHKPGGITMLQRIGTGIFLSFLAMVVAAL 432

Query: 424 VEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGLR 483
           VE+KRLKTA +YGLVD P ATVP+S+WWLVPQYVLFG+ DVF MVGLQEFFYDQVP+ LR
Sbjct: 433 VEMKRLKTAADYGLVDSPDATVPMSVWWLVPQYVLFGITDVFAMVGLQEFFYDQVPNELR 492

Query: 484 SMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSAI 543
           S+GL+LYLSIFG+GNFLSSF+IS IE+ TS  G+ SWF NNLN+AHLDYFYWLLA LS I
Sbjct: 493 SVGLALYLSIFGIGNFLSSFMISIIEKATSQSGQASWFANNLNQAHLDYFYWLLACLSFI 552

Query: 544 GLAAFLCAARTYIYNKGNTT 564
           GLA++L  A++Y+  + +T+
Sbjct: 553 GLASYLYVAKSYVSKRLDTS 557

BLAST of Lag0039769 vs. TAIR 10
Match: AT1G72120.1 (Major facilitator superfamily protein )

HSP 1 Score: 616.7 bits (1589), Expect = 1.9e-176
Identity = 310/560 (55.36%), Postives = 409/560 (73.04%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQL 61
           E+ L EE V  AVD+ G   RRSN+G WR+A  IIGVE AERFAYYG+ SNLI++LT  L
Sbjct: 6   EISLQEEYVTDAVDHRGLAARRSNTGRWRAALFIIGVEVAERFAYYGIGSNLISYLTGPL 65

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            +STA AA NVNAWSG A+LLP+LGAF+AD+FLGRYRTI +SS +Y+LGL  LT+SA L 
Sbjct: 66  GESTAVAAANVNAWSGIATLLPVLGAFVADAFLGRYRTIIISSLIYVLGLAFLTLSAFLI 125

Query: 122 SPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
             +T     T   +         VLFFFSLYLVA  Q GHKPCVQAFGADQFD +  +E 
Sbjct: 126 PNTTEVTSSTSSFLN--------VLFFFSLYLVAIGQSGHKPCVQAFGADQFDEKDSQEK 185

Query: 182 KAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
             +SSFFNWWY  LS  I  ++ ++ Y+Q+  SW+ GFGI C  MV + ++F+ G R YR
Sbjct: 186 SDRSSFFNWWYLSLSAGICFAILVVVYIQEEFSWAFGFGIPCVFMVISLVLFVSGRRIYR 245

Query: 242 YSNRRDEK--NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALV 301
           YS RR E+  NP  RIGRVF  A++N  + SS++   E    L  + S ++  F +KAL+
Sbjct: 246 YSKRRHEEEINPFTRIGRVFFVALKNQRLSSSDLCKVE----LEANTSPEKQSFFNKALL 305

Query: 302 APNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVP 361
            PN S +  +A    +VE+A A++RL P+W T LAYAI ++Q+ TFFTKQGV+MDR+I+P
Sbjct: 306 VPNDSSQGENASKSSDVEDATALIRLIPVWFTTLAYAIPYAQYMTFFTKQGVTMDRTILP 365

Query: 362 GFEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMI 421
           G +IP A LQ FI +SIV+ +P YDR+ +PIAR  T +P GIT L+RIG G+++S ++M+
Sbjct: 366 GVKIPPASLQVFIGISIVLFVPIYDRVFVPIARLITKEPCGITTLKRIGTGIVLSTITMV 425

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           +AA+VE KRL+TA+E+GL+D P+AT+P+SIWWL+PQY+L G+ADV+T+VG+QEFFY QVP
Sbjct: 426 IAALVEFKRLETAKEHGLIDQPEATLPMSIWWLIPQYLLLGLADVYTLVGMQEFFYSQVP 485

Query: 482 SGLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRS+GL+LYLS  GVG+ LSS LIS I+  T GD   SWF++NLN+AHLDYFYWLLA 
Sbjct: 486 TELRSIGLALYLSALGVGSLLSSLLISLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAI 545

Query: 542 LSAIGLAAFLCAARTYIYNK 560
           +SA+G   FL  +++YIY +
Sbjct: 546 VSAVGFFTFLFISKSYIYRR 553

BLAST of Lag0039769 vs. TAIR 10
Match: AT1G72125.1 (Major facilitator superfamily protein )

HSP 1 Score: 615.1 bits (1585), Expect = 5.4e-176
Identity = 312/557 (56.01%), Postives = 407/557 (73.07%), Query Frame = 0

Query: 5   LLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQLHQS 64
           L EE V  AVD+ G   RRS +G WR+A  IIGVE AERFA YG+ SNLI++LT  L QS
Sbjct: 9   LQEEYVIDAVDHRGFSARRSITGRWRAAWFIIGVEVAERFANYGIGSNLISYLTGPLGQS 68

Query: 65  TATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPS 124
           TA AA NVNAWSG +++LPLLGAF+AD+FLGRY TI ++S +Y+LGL  LT+SA L   +
Sbjct: 69  TAVAAANVNAWSGISTILPLLGAFVADAFLGRYITIIIASFIYVLGLAFLTLSAFLIPNN 128

Query: 125 TSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAK 184
           T         V  SP+     LFFFSLYLVA  Q GHKPCVQAFGADQFD ++P+EN  +
Sbjct: 129 T--------EVTSSPSSFLNALFFFSLYLVAIGQSGHKPCVQAFGADQFDEKNPQENSDR 188

Query: 185 SSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYSN 244
           SSFFNWWY  +   I  ++ ++ Y+Q+N+SW+LGFGI C  MV + ++F+LG ++YR+S 
Sbjct: 189 SSFFNWWYLSMCAGIGLAILVVVYIQENVSWALGFGIPCVFMVISLVLFVLGRKSYRFSK 248

Query: 245 RR--DEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVAPN 304
            R  +E NP  RIGRVF  A +N  + SS++   E I       S ++L FL+KAL+ PN
Sbjct: 249 TRQEEETNPFTRIGRVFFVAFKNQRLNSSDLCKVELIEANRSQESPEELSFLNKALLVPN 308

Query: 305 GSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPGFE 364
            S E   AC   +VE+A A++RL P+W+T LAYAI F+Q+ TFFTKQGV+M+R+I PG E
Sbjct: 309 DSDEGEVACKSRDVEDATALVRLIPVWLTTLAYAIPFAQYMTFFTKQGVTMERTIFPGVE 368

Query: 365 IPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIVAA 424
           IP A LQ  IS+SIV+ +P YDR+L+PI R+ T  P GIT L+RIG GM+++ L+M+VAA
Sbjct: 369 IPPASLQVLISISIVLFVPIYDRVLVPIGRSITKDPCGITTLKRIGTGMVLATLTMVVAA 428

Query: 425 VVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSGL 484
           +VE KRL+TA+EYGL+D PK T+P+SIWWL PQY+L G+ADV T+VG+QEFFY QVP+ L
Sbjct: 429 LVESKRLETAKEYGLIDQPKTTLPMSIWWLFPQYMLLGLADVHTLVGMQEFFYSQVPTEL 488

Query: 485 RSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLSA 544
           RS+GL++YLS  GVG+ LSS LI  I+  T GD   SWF++NLN+AHLDYFYWLLA +SA
Sbjct: 489 RSLGLAIYLSAMGVGSLLSSLLIYLIDLATGGDAGNSWFNSNLNRAHLDYFYWLLAVVSA 548

Query: 545 IGLAAFLCAARTYIYNK 560
           +G   FL  +++YIY +
Sbjct: 549 VGFFTFLFISKSYIYRR 557

BLAST of Lag0039769 vs. TAIR 10
Match: AT1G22550.1 (Major facilitator superfamily protein )

HSP 1 Score: 607.4 bits (1565), Expect = 1.1e-173
Identity = 308/560 (55.00%), Postives = 405/560 (72.32%), Query Frame = 0

Query: 2   EVPLLEETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQL 61
           E  L+E++V  +VD+ G P  +S++GGWRSA  IIGVE  ERFAY+G+ SNLI +LT  L
Sbjct: 7   EAALIEDSVSDSVDHRGLPAGKSSTGGWRSAWYIIGVEVGERFAYFGIGSNLITYLTGPL 66

Query: 62  HQSTATAAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLP 121
            QSTATAA NVN WSG AS+LP+LGAF+AD++LGRYRTI ++S +YILGLGLLT+S+ L 
Sbjct: 67  GQSTATAAVNVNTWSGTASILPVLGAFIADAYLGRYRTIVVASLIYILGLGLLTLSSILI 126

Query: 122 SPSTSACQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEEN 181
               S  +Q  ++ +  P     +LFF SLYLVA  QGGHKPCVQAFGADQFD   P+E 
Sbjct: 127 LMGLSEQRQHNRNASAKPFFWVNILFFCSLYLVAIGQGGHKPCVQAFGADQFDVGDPKER 186

Query: 182 KAKSSFFNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYR 241
            ++ SFFNWW+  LS  I  S+ ++ YVQDN++W+LGFGI C  MV A  +FL G +TYR
Sbjct: 187 ISRGSFFNWWFLSLSAGITLSIIVVVYVQDNVNWALGFGIPCLFMVMALALFLFGRKTYR 246

Query: 242 Y--SNRRDEKNPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALV 301
           Y   +R  + N   RIGRVF+ A +N  +K   + H  ++          QL FL KAL+
Sbjct: 247 YPRGDREGKNNAFARIGRVFLVAFKNRKLK---LTHSGQLEVGSYKKCKGQLEFLAKALL 306

Query: 302 APNGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVP 361
              G  E    CS  +VE+A A++RL PIW+T +   I ++Q++TFFTKQGV++DR I+P
Sbjct: 307 PGEGGVE---PCSSRDVEDAMALVRLIPIWITSVVSTIPYAQYATFFTKQGVTVDRKILP 366

Query: 362 GFEIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMI 421
           GFEIP A  Q+ I LSI I +PTY+R+ +P+AR  T KPSGITMLQRIG GM++S+L+M+
Sbjct: 367 GFEIPPASFQALIGLSIFISVPTYERVFLPLARLITKKPSGITMLQRIGAGMVLSSLNMV 426

Query: 422 VAAVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVP 481
           VAA+VE+KRL+TA+E+GLVD P AT+P+SIWW VPQY+L G+ DVF++VG QEFFYDQVP
Sbjct: 427 VAALVEMKRLETAKEHGLVDRPDATIPMSIWWFVPQYLLLGMIDVFSLVGTQEFFYDQVP 486

Query: 482 SGLRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAG 541
           + LRS+GL+L LS  G+ +FLS FLI+ I   T  +G +SWF+ NLN+AH+DYFYWLLA 
Sbjct: 487 TELRSIGLALSLSAMGLASFLSGFLITVINWATGKNGGDSWFNTNLNRAHVDYFYWLLAA 546

Query: 542 LSAIGLAAFLCAARTYIYNK 560
            +AIG  AFL  +R Y+Y +
Sbjct: 547 FTAIGFLAFLLLSRLYVYRR 560

BLAST of Lag0039769 vs. TAIR 10
Match: AT1G72140.1 (Major facilitator superfamily protein )

HSP 1 Score: 596.3 bits (1536), Expect = 2.6e-170
Identity = 306/557 (54.94%), Postives = 406/557 (72.89%), Query Frame = 0

Query: 8   ETVEGAVDYSGRPVRRSNSGGWRSASLIIGVEAAERFAYYGVSSNLINFLTDQLHQSTAT 67
           E VE +VD+ G P  RS+SG W+S+   +  E AE+FAY+G++SNLI + T+ L +STA 
Sbjct: 17  ENVEFSVDFRGNPSIRSSSGAWKSSGFTMCAEVAEKFAYFGIASNLITYFTEALGESTAV 76

Query: 68  AAENVNAWSGAASLLPLLGAFLADSFLGRYRTIALSSALYILGLGLLTVSATLPSPSTSA 127
           AA NVN W G A+ LPL+   +ADSFLGR+RTI L+S+ YI+GLGLLT SAT+P    S 
Sbjct: 77  AASNVNLWLGTAAFLPLIWGSIADSFLGRFRTILLTSSFYIMGLGLLTFSATIP----SL 136

Query: 128 CQQTEKSVACSPTLVQLVLFFFSLYLVAFAQGGHKPCVQAFGADQFDGQHPEENKAKSSF 187
           C   E   +C  + V++++FF +LYL+A  +GG K C++AFGADQFD Q P E+KAKSS+
Sbjct: 137 CNDQETRESC-VSQVKVIIFFCALYLIALGEGGFKVCLRAFGADQFDEQDPNESKAKSSY 196

Query: 188 FNWWYFGLSLAILASVNILNYVQDNLSWSLGFGISCTAMVFAFLVFLLGTRTYRYS---- 247
           FNW YF +S+ IL +  + NYVQ+NLSW+LG+ I C +M+ A  +FLLG +TYR+S    
Sbjct: 197 FNWLYFAISIGILTTRLVTNYVQENLSWALGYAIPCLSMMLALFLFLLGIKTYRFSTGGE 256

Query: 248 NRRDEK--NPLVRIGRVFITAVRNWHVKSSEIAHEEEIRGLLPHHSSQQLRFLDKALVAP 307
            R+ +K  NP VRIGRVF+ A RN     S+         LLP+ S+++ RFLD+A++  
Sbjct: 257 GRQGKKHDNPFVRIGRVFVAAARNRRQTPSDTCL------LLPNESTKKFRFLDRAVI-- 316

Query: 308 NGSKEDGHACSVGEVEEAKAVLRLAPIWVTCLAYAIVFSQFSTFFTKQGVSMDRSIVPGF 367
                   +C   EVEEAKAVL L PIW+  L + IVF+Q  TFFTKQG +MDRSI    
Sbjct: 317 --------SCDSYEVEEAKAVLSLIPIWLCSLVFGIVFAQSPTFFTKQGSTMDRSISSTL 376

Query: 368 EIPAAWLQSFISLSIVICLPTYDRILIPIARNFTGKPSGITMLQRIGFGMLVSALSMIVA 427
           ++PAA LQ FISL+I++ +P YDR+ +PIAR+ T KP+GIT LQRI  G+ +S +SM++A
Sbjct: 377 QVPAATLQCFISLAILVFIPIYDRLFVPIARSITRKPAGITTLQRISTGIFLSIISMVIA 436

Query: 428 AVVEIKRLKTAQEYGLVDLPKATVPLSIWWLVPQYVLFGVADVFTMVGLQEFFYDQVPSG 487
           A+VE+KRLKTA+++GLVD PKATVP+S+ WL+PQY+LFGV+DVFTMVGLQEFFY +VP  
Sbjct: 437 ALVEMKRLKTARDHGLVDSPKATVPMSVCWLIPQYILFGVSDVFTMVGLQEFFYGEVPPQ 496

Query: 488 LRSMGLSLYLSIFGVGNFLSSFLISAIERLTSGDGRESWFDNNLNKAHLDYFYWLLAGLS 547
           LRSMGL+LYLSI G+GNFLSSF++S IE  TS  G+ SWF NNLN+AHLDYFYWLLA LS
Sbjct: 497 LRSMGLALYLSIIGIGNFLSSFMVSVIEEATSQSGQVSWFSNNLNQAHLDYFYWLLACLS 552

Query: 548 AIGLAAFLCAARTYIYN 559
           ++     +  A++Y+YN
Sbjct: 557 SLAFIFTVYFAKSYLYN 552

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023535812.12.2e-28086.32protein NRT1/ PTR FAMILY 5.10-like isoform X4 [Cucurbita pepo subsp. pepo][more]
XP_023535811.13.8e-28086.15protein NRT1/ PTR FAMILY 5.10-like isoform X3 [Cucurbita pepo subsp. pepo][more]
XP_022973964.11.9e-27986.50protein NRT1/ PTR FAMILY 5.10-like [Cucurbita maxima][more]
XP_023535809.13.2e-27986.15protein NRT1/ PTR FAMILY 5.10-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_0... [more]
XP_022925410.13.2e-27986.50protein NRT1/ PTR FAMILY 5.10-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q0WP012.3e-20866.07Protein NRT1/ PTR FAMILY 5.10 OS=Arabidopsis thaliana OX=3702 GN=NPF5.10 PE=2 SV... [more]
Q8VZE22.6e-17555.36Protein NRT1/ PTR FAMILY 5.14 OS=Arabidopsis thaliana OX=3702 GN=NPF5.14 PE=2 SV... [more]
Q0WSZ67.6e-17556.01Protein NRT1/ PTR FAMILY 5.13 OS=Arabidopsis thaliana OX=3702 GN=NPF5.13 PE=2 SV... [more]
Q9SK961.6e-17255.00Protein NRT1/ PTR FAMILY 5.16 OS=Arabidopsis thaliana OX=3702 GN=NPF5.16 PE=2 SV... [more]
Q9C7U13.7e-16954.94Protein NRT1/ PTR FAMILY 5.12 OS=Arabidopsis thaliana OX=3702 GN=NPF5.12 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1IG549.1e-28086.50protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita maxima OX=3661 GN=LOC111472583 P... [more]
A0A6J1EHW21.5e-27986.50protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432710... [more]
A0A6J1EC561.4e-27785.79protein NRT1/ PTR FAMILY 5.10-like OS=Cucurbita moschata OX=3662 GN=LOC111432731... [more]
A0A1S3ATI36.1e-27685.41protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo OX=3656 GN=LOC103482564 PE=3 ... [more]
A0A5A7TJ766.1e-27685.41Protein NRT1/ PTR FAMILY 5.10-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
Match NameE-valueIdentityDescription
AT1G22540.11.7e-20966.07Major facilitator superfamily protein [more]
AT1G72120.11.9e-17655.36Major facilitator superfamily protein [more]
AT1G72125.15.4e-17656.01Major facilitator superfamily protein [more]
AT1G22550.11.1e-17355.00Major facilitator superfamily protein [more]
AT1G72140.12.6e-17054.94Major facilitator superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000109Proton-dependent oligopeptide transporter familyPFAMPF00854PTR2coord: 97..513
e-value: 1.0E-94
score: 317.6
IPR000109Proton-dependent oligopeptide transporter familyPANTHERPTHR11654OLIGOPEPTIDE TRANSPORTER-RELATEDcoord: 16..560
IPR036259MFS transporter superfamilyGENE3D1.20.1250.20MFS general substrate transporter like domainscoord: 5..562
e-value: 2.4E-201
score: 671.5
IPR036259MFS transporter superfamilySUPERFAMILY103473MFS general substrate transportercoord: 20..553
NoneNo IPR availablePANTHERPTHR11654:SF510FACILITATOR SUPERFAMILY PROTEIN, PUTATIVE ISOFORM 1-RELATEDcoord: 16..560
IPR018456PTR2 family proton/oligopeptide symporter, conserved sitePROSITEPS01022PTR2_1coord: 86..110
IPR044739NRT1/PTR familyCDDcd17417MFS_NPF5coord: 29..557
e-value: 0.0
score: 698.25

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039769.1Lag0039769.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035442 dipeptide transmembrane transport
biological_process GO:0042939 tripeptide transport
biological_process GO:0042938 dipeptide transport
biological_process GO:0006857 oligopeptide transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0071916 dipeptide transmembrane transporter activity
molecular_function GO:0042937 tripeptide transmembrane transporter activity
molecular_function GO:0022857 transmembrane transporter activity