Lag0039492 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039492
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr2: 44818314 .. 44818715 (-)
RNA-Seq ExpressionLag0039492
SyntenyLag0039492
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGTTGGAAAGTCACTAACCGAGAATCTTGATGAGTTCAAAAAGATGACATCCGAGTTCAGGAATCTTGGAGAGAAGATAGGGGATGATAACAAGCCTTTTGTTCTATTAAATTCACTTCCTGATGCTTATAAGGAAGTGAAGACTGCCCTCAAATATGGAAGAGAGTCCATTACTACTTATGCTATTATATCAGCTATCAGAACCAAGGAATTGAAGTTGTTGTCTATAAAGAAAGAGGCTACTAAGGGTTTGTTTGTCAAAGGTACAACCAGAAATAAAGAGGGGAAAAATCAGTCAGATGATAAGGGCAATACCAAGGTTAAGTGCAACTACTGTTATAAGGAGGGGCATATCAAGAGGGAATGTTATTCTTTAAAAAGGAAGAACCAAAACTAG

mRNA sequence

ATGGATGTTGGAAAGTCACTAACCGAGAATCTTGATGAGTTCAAAAAGATGACATCCGAGTTCAGGAATCTTGGAGAGAAGATAGGGGATGATAACAAGCCTTTTGTTCTATTAAATTCACTTCCTGATGCTTATAAGGAAGTGAAGACTGCCCTCAAATATGGAAGAGAGTCCATTACTACTTATGCTATTATATCAGCTATCAGAACCAAGGAATTGAAGTTGTTGTCTATAAAGAAAGAGGCTACTAAGGGTTTGTTTGTCAAAGGTACAACCAGAAATAAAGAGGGGAAAAATCAGTCAGATGATAAGGGCAATACCAAGGTTAAGTGCAACTACTGTTATAAGGAGGGGCATATCAAGAGGGAATGTTATTCTTTAAAAAGGAAGAACCAAAACTAG

Coding sequence (CDS)

ATGGATGTTGGAAAGTCACTAACCGAGAATCTTGATGAGTTCAAAAAGATGACATCCGAGTTCAGGAATCTTGGAGAGAAGATAGGGGATGATAACAAGCCTTTTGTTCTATTAAATTCACTTCCTGATGCTTATAAGGAAGTGAAGACTGCCCTCAAATATGGAAGAGAGTCCATTACTACTTATGCTATTATATCAGCTATCAGAACCAAGGAATTGAAGTTGTTGTCTATAAAGAAAGAGGCTACTAAGGGTTTGTTTGTCAAAGGTACAACCAGAAATAAAGAGGGGAAAAATCAGTCAGATGATAAGGGCAATACCAAGGTTAAGTGCAACTACTGTTATAAGGAGGGGCATATCAAGAGGGAATGTTATTCTTTAAAAAGGAAGAACCAAAACTAG

Protein sequence

MDVGKSLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALKYGRESITTYAIISAIRTKELKLLSIKKEATKGLFVKGTTRNKEGKNQSDDKGNTKVKCNYCYKEGHIKRECYSLKRKNQN
Homology
BLAST of Lag0039492 vs. NCBI nr
Match: XP_038885928.1 (uncharacterized protein LOC120076236 [Benincasa hispida])

HSP 1 Score: 120.6 bits (301), Expect = 1.1e-23
Identity = 64/132 (48.48%), Postives = 92/132 (69.70%), Query Frame = 0

Query: 1   MDVGKSLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALKYGRESIT 60
           MD  KSLT+NL+EFK ++S+FR++G+ IG++N+ F+LLNSLP+ +K+VKTALKYGRE IT
Sbjct: 112 MDPAKSLTDNLNEFKSLSSDFRSIGDNIGEENEAFILLNSLPETFKDVKTALKYGRERIT 171

Query: 61  TYAIISAIRTKELKLLSIKKEATK--GLFVKGTTRNKEGKNQSDDKGNTKVKCNYCYKEG 120
           TYAIISA+  KEL+L   KK+  +  G F KG  +     N++            C ++ 
Sbjct: 172 TYAIISAVSVKELELQMTKKDQPRGEGFFPKGNNKTMGRANRT---------MEMCIRDS 231

Query: 121 HIKRECYSLKRK 131
            +K++CY+LKRK
Sbjct: 232 -MKKDCYALKRK 233

BLAST of Lag0039492 vs. NCBI nr
Match: KAA0065687.1 (retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa] >TYK08214.1 retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 112.5 bits (280), Expect = 2.9e-21
Identity = 57/92 (61.96%), Postives = 75/92 (81.52%), Query Frame = 0

Query: 1   MDVGKSLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALKYGRESIT 60
           MD  KSL ENL EFKK++S+F+ LG+KIGD+N+ F+LLNSLP+AYKEVK AL+YGR+ IT
Sbjct: 29  MDDNKSLIENLGEFKKLSSDFKELGDKIGDENESFILLNSLPEAYKEVKMALRYGRQKIT 88

Query: 61  TYAIISAIRTKELKLLSIKKEAT--KGLFVKG 91
           T  +ISAIRT+EL L S +++ +  +GLF KG
Sbjct: 89  TGGVISAIRTRELDLNSQRRDQSNGEGLFSKG 120

BLAST of Lag0039492 vs. NCBI nr
Match: XP_022152111.1 (uncharacterized protein LOC111019900 [Momordica charantia])

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-20
Identity = 52/75 (69.33%), Postives = 68/75 (90.67%), Query Frame = 0

Query: 1  MDVGKSLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALKYGRESIT 60
          MD  K+L++NLD+FKK++SEF +LGEKIG +N+ F+LLNSLP++Y+EVK ALKYGRESIT
Sbjct: 1  MDSSKTLSDNLDDFKKLSSEFNSLGEKIGAENEAFILLNSLPESYREVKVALKYGRESIT 60

Query: 61 TYAIISAIRTKELKL 76
          T AIISA++TKEL+L
Sbjct: 61 TDAIISAVKTKELEL 75

BLAST of Lag0039492 vs. NCBI nr
Match: TYK27723.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 110.2 bits (274), Expect = 1.4e-20
Identity = 58/128 (45.31%), Postives = 83/128 (64.84%), Query Frame = 0

Query: 1   MDVGKSLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALKYGRESIT 60
           M+  K+L ENLDEFKK+T+      EK+G +++  +L+N + D YKEVKT+LKYGRE+IT
Sbjct: 112 MNQNKNLDENLDEFKKLTNALNQTREKLGAESEAAILINLIHDTYKEVKTSLKYGRETIT 171

Query: 61  TYAIISAIRTKELKLLSIKK--EATKGLFVKGTT--RNKEGKNQSDDKGNTKVKCNYCYK 120
             ++I+A+++KEL+L +  K   A + LF KG    R    KNQ   +    +KC  C+K
Sbjct: 172 VNSVITALKSKELELKTENKTSNAAESLFSKGNNSFRKNSNKNQRSSRDKPALKCFICHK 231

Query: 121 EGHIKREC 125
           EGH KR C
Sbjct: 232 EGHFKRNC 239

BLAST of Lag0039492 vs. NCBI nr
Match: KAA0043826.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 109.8 bits (273), Expect = 1.9e-20
Identity = 55/128 (42.97%), Postives = 85/128 (66.41%), Query Frame = 0

Query: 1   MDVGKSLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALKYGRESIT 60
           MD  KSL ENLDEF+K+  +  N+GEK+ D+N+  +LLNSLP+ Y+EVK A+KYGR+S+T
Sbjct: 1   MDQSKSLEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 60

Query: 61  TYAIISAIRTKELKLLSIKKEA----TKGLFVKGTTRNKEGKNQSDDKGNTKVKCNYCYK 120
              ++ A++T+ L++   +K+      +G   K + + KE   +S  KG ++ KC  C+K
Sbjct: 61  MSIVLDALKTRNLEIKKERKDGELLMARGRSEKKSWKGKERSFRSKSKGKSR-KCFLCHK 120

Query: 121 EGHIKREC 125
           EGH K+ C
Sbjct: 121 EGHFKKNC 127

BLAST of Lag0039492 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 3.2e-07
Identity = 36/135 (26.67%), Postives = 69/135 (51.11%), Query Frame = 0

Query: 1   MDVGKSLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALKYGRESIT 60
           M  G +   +L+ F  + ++  NLG KI +++K  +LLNSLP +Y  + T + +G+ +I 
Sbjct: 112 MSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHGKTTIE 171

Query: 61  TYAIISAIRTKELKLLSIKKEATKGLFVKGTTRNKE----------GKNQSDDKGNTKVK 120
              + SA+   E K+    +   + L  +G  R+ +           + +S ++  ++V+
Sbjct: 172 LKDVTSALLLNE-KMRKKPENQGQALITEGRGRSYQRSSNNYGRSGARGKSKNRSKSRVR 231

Query: 121 -CNYCYKEGHIKREC 125
            C  C + GH KR+C
Sbjct: 232 NCYNCNQPGHFKRDC 245

BLAST of Lag0039492 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 52.8 bits (125), Expect = 3.6e-06
Identity = 39/138 (28.26%), Postives = 70/138 (50.72%), Query Frame = 0

Query: 6   SLTENLDEFKKMTSEFRNLGEKIGDDNKPFVLLNSLPDAYKEVKTALK-YGRESITTYAI 65
           SL  +   F ++ SE    G KI + +K   LL +LP  Y  + TA++    E++T   +
Sbjct: 113 SLLSHFHIFDELISELLAAGAKIEEMDKISHLLITLPSCYDGIITAIETLSEENLTLAFV 172

Query: 66  ISAIRTKELKLLSIKKEATK-----------GLFVKGTTRNKEGKNQSDDKGNT--KVKC 125
            + +  +E+K+ +   + +K             +     +N+  K +   KGN+  KVKC
Sbjct: 173 KNRLLDQEIKIKNDHNDTSKKVMNAIVHNNNNTYKNNLFKNRVTKPKKIFKGNSKYKVKC 232

Query: 126 NYCYKEGHIKRECYSLKR 130
           ++C +EGHIK++C+  KR
Sbjct: 233 HHCGREGHIKKDCFHYKR 250

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885928.11.1e-2348.48uncharacterized protein LOC120076236 [Benincasa hispida][more]
KAA0065687.12.9e-2161.96retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
XP_022152111.11.4e-2069.33uncharacterized protein LOC111019900 [Momordica charantia][more]
TYK27723.11.4e-2045.31Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
KAA0043826.11.9e-2042.97putative gag-pol polyprotein [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
P109783.2e-0726.67Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041463.6e-0628.26Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D4.10.60.10coord: 87..133
e-value: 8.1E-6
score: 27.6
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 1..73
e-value: 2.1E-10
score: 40.5
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 110..124
score: 8.531888
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 92..130

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039492.1Lag0039492.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding