Lag0039485 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0039485
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr2: 44704244 .. 44705361 (-)
RNA-Seq ExpressionLag0039485
SyntenyLag0039485
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTCCAAGAATTTTTTCAAAACGGTATCGTCAACAAGCGTACCAATGAAACTTATATTTGTTTGATTCCCAAGAAGAAAAAGGTTGACAAGGTTAATGATTTTAGACCTATAAGTCCACTGACCTCCCCCTACAAAATTTTAGCCAAAGTGTTAGCTGAAAGGCTAAAAAAAGTGCTCCCTCATACTATTAGCGATTGTCAAGCCGCCTTTGTGCATGGTAGGCAATTTCTTGATGTTATTTTGGTTGCTACGGAAGCTGTGGAAGATATGAAAGCTAGAAAAGAGGAGGGAGTGTTGGTTAAACTTGATCTAGAGAAGGCCTATTATATGGTGAGTTGGGAGGTCCTAGAAGCGATCCTTAAATTGAAAGGTTTTGGCTCTAAATGGAGATTATGGATTAAAGGTTGTCTTAGAAAAACAAACTTTTCAGTGTTTTTAAATGGAAGACCTAGAGGGAAGATTATGGCTATTAGAGGGTTGAGGCAAGGGGATCCACTCTCCCCCTTCCTTTTCACTATAGTTGGTGATGTTATTAGTAAATCTGTGCAACTTTGTCTTGAGAAGAAAATTCTGAATGGTTGGTTGTTGGGCAAAGCAAATAAGTAGTCTCTATCTTACAGTATGCTGATGACACGCTGATTTTTAGCCCCAATTGTGAATCTGAGTTGAGTAGATGGTGGGACATTCTCAAACTTATTATGGCAGGTTCGGGCTTGAGTATCAACATGAACAAAACGTCCATGATAAGCATCAATAATGATAGATCTATGGTTGCTCAGTGGGCAAACTCCTTTGGCTACCAATCGAGTTCTCTTCCTTTTAATTATCTTGGCTTCCCCCTAGGTGGAAATTACCATAGAAAATCCTTTTGGGATCCAATGCTAGAAAAGTTCAACAGAAAGCTTGACATGTGGAAGAGGTTTTCTCTTTCCAAAGGGGGCAGAGTCACGCTAGCTGAATCTGTGCTTAACAGCTTACCCATTTATTATTTCTCCCTGCTAAAATCCCCGAAAGGTGTCATCTCGAAGATGGAAAAGGTGACAAGAGACTTTGTTTGGAACGAAGGAAGTTACAAGCGCGACTCTAACTTGGTTAAATGGGAGTGGACCGTTTGA

mRNA sequence

ATGTTCCAAGAATTTTTTCAAAACGGTATCGTCAACAAGCGTACCAATGAAACTTATATTTGTTTGATTCCCAAGAAGAAAAAGGTTGACAAGGTTAATGATTTTAGACCTATAAGTCCACTGACCTCCCCCTACAAAATTTTAGCCAAAGTGTTAGCTGAAAGGCTAAAAAAAGTGCTCCCTCATACTATTAGCGATTGTCAAGCCGCCTTTGTGCATGGTAGGCAATTTCTTGATGTTATTTTGGTTGCTACGGAAGCTGTGGAAGATATGAAAGCTAGAAAAGAGGAGGGAGTGTTGGTTAAACTTGATCTAGAGAAGGCCTATTATATGTATGCTGATGACACGCTGATTTTTAGCCCCAATTGTGAATCTGAGTTGAGTAGATGGTGGGACATTCTCAAACTTATTATGGCAGGTTCGGGCTTGAGTATCAACATGAACAAAACGTCCATGATAAGCATCAATAATGATAGATCTATGGTTGCTCAGTGGGCAAACTCCTTTGGCTACCAATCGAGTTCTCTTCCTTTTAATTATCTTGGCTTCCCCCTAGGTGGAAATTACCATAGAAAATCCTTTTGGGATCCAATGCTAGAAAAGTTCAACAGAAAGCTTGACATGTGGAAGAGGTTTTCTCTTTCCAAAGGGGGCAGAGTCACGCTAGCTGAATCTGTGCTTAACAGCTTACCCATTTATTATTTCTCCCTGCTAAAATCCCCGAAAGGTGTCATCTCGAAGATGGAAAAGGTGACAAGAGACTTTGTTTGGAACGAAGGAAGTTACAAGCGCGACTCTAACTTGGTTAAATGGGAGTGGACCGTTTGA

Coding sequence (CDS)

ATGTTCCAAGAATTTTTTCAAAACGGTATCGTCAACAAGCGTACCAATGAAACTTATATTTGTTTGATTCCCAAGAAGAAAAAGGTTGACAAGGTTAATGATTTTAGACCTATAAGTCCACTGACCTCCCCCTACAAAATTTTAGCCAAAGTGTTAGCTGAAAGGCTAAAAAAAGTGCTCCCTCATACTATTAGCGATTGTCAAGCCGCCTTTGTGCATGGTAGGCAATTTCTTGATGTTATTTTGGTTGCTACGGAAGCTGTGGAAGATATGAAAGCTAGAAAAGAGGAGGGAGTGTTGGTTAAACTTGATCTAGAGAAGGCCTATTATATGTATGCTGATGACACGCTGATTTTTAGCCCCAATTGTGAATCTGAGTTGAGTAGATGGTGGGACATTCTCAAACTTATTATGGCAGGTTCGGGCTTGAGTATCAACATGAACAAAACGTCCATGATAAGCATCAATAATGATAGATCTATGGTTGCTCAGTGGGCAAACTCCTTTGGCTACCAATCGAGTTCTCTTCCTTTTAATTATCTTGGCTTCCCCCTAGGTGGAAATTACCATAGAAAATCCTTTTGGGATCCAATGCTAGAAAAGTTCAACAGAAAGCTTGACATGTGGAAGAGGTTTTCTCTTTCCAAAGGGGGCAGAGTCACGCTAGCTGAATCTGTGCTTAACAGCTTACCCATTTATTATTTCTCCCTGCTAAAATCCCCGAAAGGTGTCATCTCGAAGATGGAAAAGGTGACAAGAGACTTTGTTTGGAACGAAGGAAGTTACAAGCGCGACTCTAACTTGGTTAAATGGGAGTGGACCGTTTGA

Protein sequence

MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVLPHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAYYMYADDTLIFSPNCESELSRWWDILKLIMAGSGLSINMNKTSMISINNDRSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKLDMWKRFSLSKGGRVTLAESVLNSLPIYYFSLLKSPKGVISKMEKVTRDFVWNEGSYKRDSNLVKWEWTV
Homology
BLAST of Lag0039485 vs. NCBI nr
Match: VVA31869.1 (Hypothetical predicted protein, partial [Prunus dulcis])

HSP 1 Score: 221.9 bits (564), Expect = 7.0e-54
Identity = 128/341 (37.54%), Postives = 179/341 (52.49%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVL 60
           + Q+FFQ+GIVN  TNET+ICLIPKK    KV DFRPIS +TS YK+++KVLA RL++VL
Sbjct: 130 VMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDFRPISLVTSLYKVISKVLASRLREVL 189

Query: 61  PHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY----------- 120
            +TIS  Q AFV  RQ LD +LVA E VE+++ +  +G++ K+D EKAY           
Sbjct: 190 GNTISQSQGAFVQKRQILDAVLVANEVVEEVRKQNRKGLVFKIDFEKAYDHVEWNFVDDV 249

Query: 121 -----------------------------------------------------------Y 180
                                                                       
Sbjct: 250 LARKGFGFRASRGLRQGDPLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHDQVEVSHL 309

Query: 181 MYADDTLIFSPNCESELSRWWDILKLIMAGSGLSINMNKTSMISINNDRSMVAQWANSFG 240
            +ADDT+ F    E        +LKL    SG+ IN  K+ ++ IN     +   A S+G
Sbjct: 310 QFADDTIFFLDGKEEYWLNLLQMLKLFCDVSGMKINKAKSCILGINFSIETLNNMAGSWG 369

Query: 241 YQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKLDMWKRFSLSKGGRVTLAESVLNSL 272
            +    P  YLG PLGGN    +FW+P+L+K  ++L  WKR  LSKGGR+TL ++VL+S+
Sbjct: 370 CEVGCWPMVYLGLPLGGNPRALNFWNPVLDKVEKRLQRWKRACLSKGGRLTLIQAVLSSI 429

BLAST of Lag0039485 vs. NCBI nr
Match: CAN66242.1 (hypothetical protein VITISV_015915 [Vitis vinifera])

HSP 1 Score: 221.5 bits (563), Expect = 9.1e-54
Identity = 118/295 (40.00%), Postives = 180/295 (61.02%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVL 60
           +F EF ++ I+N+ TN ++I L+PKK    +++D+RPIS +TS YKI+AKVLA RL+ VL
Sbjct: 275 VFVEFHRSRIINQSTNASFIVLLPKKSMSKRISDYRPISLITSLYKIIAKVLARRLRGVL 334

Query: 61  PHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY----------- 120
             TI   Q AFV GRQ LD +L+A E V++ +   EEGV+ K+D EKAY           
Sbjct: 335 HKTIHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEGVVFKIDFEKAYDHVSWDFLDHV 394

Query: 121 -----------YMYADDTLIFSPNCESELSRWWDILKLIMAGSGLSINMNKTSMISINND 180
                          DDT+ FS   E ++    ++L +    SGL +N++K+++  IN +
Sbjct: 395 LEMKGFSPRWRKWMRDDTIFFSSTREEDMMTLKNVLLVFGHISGLKVNLDKSNIYGINLE 454

Query: 181 RSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKLDMWKRFSLSKGG 240
           ++ +++ A     ++S  P  YLG PLGGN     FWDP++E+ +R+LD W++  LS GG
Sbjct: 455 QNHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVIERISRRLDGWQKTYLSFGG 514

Query: 241 RVTLAESVLNSLPIYYFSLLKSPKGVISKMEKVTRDFVWNE-GSYKRDSNLVKWE 273
           R+TL +S L  +P Y+ SL K P  + +K+E++ RDF+W+  G  KRD +LV W+
Sbjct: 515 RITLIQSCLTHMPCYFLSLFKIPASMAAKIERLQRDFLWSSVGEGKRD-HLVSWD 568

BLAST of Lag0039485 vs. NCBI nr
Match: XP_009335445.1 (PREDICTED: uncharacterized protein LOC103928158 [Pyrus x bretschneideri])

HSP 1 Score: 221.1 bits (562), Expect = 1.2e-53
Identity = 132/369 (35.77%), Postives = 177/369 (47.97%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVL 60
           + +EFF+ GI+N  TNET+ICLIPKK    KV D+RPIS +T  YKI+AK L  RL++V+
Sbjct: 298 VMEEFFETGIINAVTNETFICLIPKKSDSLKVTDYRPISLVTGLYKIVAKTLVSRLREVM 357

Query: 61  PHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY----------- 120
             TIS  Q  FV GRQ LD +L+A E VE+++ +KEEG++ K+D EKAY           
Sbjct: 358 GSTISPHQGGFVKGRQILDAVLIANELVEEVRQKKEEGLMFKIDFEKAYDHVEWRFLDEI 417

Query: 121 ------------------------------------------------------------ 180
                                                                       
Sbjct: 418 LQRKGFGNRWRKWVQGCQNSANFSILINGRPKGKFHASRRLRQGDPLSPFLFTLVVDVLS 477

Query: 181 --------------------------YMYADDTLIFSPNCESELSRWWDILKLIMAGSGL 240
                                       +ADDT+ F    E   +   ++L L    SGL
Sbjct: 478 RLMEKAHENHLIKGLGIGQERVEISHLQFADDTIFFLAEDEDVWNNLLEVLNLFCTISGL 537

Query: 241 SINMNKTSMISINNDRSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFN 273
            IN  K SM  IN+D   +   A+S+G +  S P  YLG PLGG      FWDP++EK  
Sbjct: 538 KINKAKCSMAGINSDCEKLKGMADSWGCEVGSWPIKYLGLPLGGRPKALMFWDPVVEKME 597

BLAST of Lag0039485 vs. NCBI nr
Match: BBN69746.1 (VIRB2-interacting protein 2 [Prunus dulcis])

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-52
Identity = 133/366 (36.34%), Postives = 182/366 (49.73%), Query Frame = 0

Query: 4   EFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVLPHT 63
           +FF  GI+N  TNET+ICLIPKKK+  KV+DFRPIS +TS YK+++KVLA RL++VL  T
Sbjct: 236 DFFNCGIINAITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGST 295

Query: 64  ISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY-------------- 123
           IS  Q+AFV GRQ LD  L+A E VE+ +   + G++ K+DLEKAY              
Sbjct: 296 ISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIR 355

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 356 KGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIM 415

Query: 184 -----------------------YMYADDTLIFSPNCESELSRWWDILKLIMAGSGLSIN 243
                                    +ADDT+ F  + E   +    IL+L    SG++IN
Sbjct: 416 EKAQDADEFHGLSPGNGMVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTIN 475

Query: 244 MNKTSMISINNDRSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKL 273
            +K S++ IN D  MV + A ++G      P  YLG PLGGN     FWDP++EK   +L
Sbjct: 476 KSKCSLVGINLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRL 535

BLAST of Lag0039485 vs. NCBI nr
Match: VVA21938.1 (Hypothetical predicted protein, partial [Prunus dulcis])

HSP 1 Score: 218.0 bits (554), Expect = 1.0e-52
Identity = 133/366 (36.34%), Postives = 182/366 (49.73%), Query Frame = 0

Query: 4   EFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVLPHT 63
           +FF  GI+N  TNET+ICLIPKKK+  KV+DFRPIS +TS YK+++KVLA RL++VL  T
Sbjct: 416 DFFNCGIINAITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGST 475

Query: 64  ISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY-------------- 123
           IS  Q+AFV GRQ LD  L+A E VE+ +   + G++ K+DLEKAY              
Sbjct: 476 ISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIR 535

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 536 KGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIM 595

Query: 184 -----------------------YMYADDTLIFSPNCESELSRWWDILKLIMAGSGLSIN 243
                                    +ADDT+ F  + E   +    IL+L    SG++IN
Sbjct: 596 EKAQDADEFHGLSPGNGMVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTIN 655

Query: 244 MNKTSMISINNDRSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKL 273
            +K S++ IN D  MV + A ++G      P  YLG PLGGN     FWDP++EK   +L
Sbjct: 656 KSKCSLVGINLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRL 715

BLAST of Lag0039485 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 64.7 bits (156), Expect = 1.9e-09
Identity = 24/80 (30.00%), Postives = 54/80 (67.50%), Query Frame = 0

Query: 192 KSFWDPMLEKFNRKLDMWKRFSLSKGGRVTLAESVLNSLPIYYFSLLKSPKGVISKMEKV 251
           K  +  +LE+ + ++  W+  +LS  GR+TL ++VL+S+P++  S +  P+ ++++++++
Sbjct: 10  KDTFGEILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQL 69

Query: 252 TRDFVWNEGSYKRDSNLVKW 272
           +R F+W   + K+  +LVKW
Sbjct: 70  SRTFLWGSTAEKKKQHLVKW 89

BLAST of Lag0039485 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.1e-06
Identity = 39/145 (26.90%), Postives = 68/145 (46.90%), Query Frame = 0

Query: 4   EFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVLPHT 63
           E F+ G +        + L+PKK  +  + ++RP+S L++ YKI+AK ++ RLK VL   
Sbjct: 494 EAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEV 553

Query: 64  ISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAYYMYADDTLIFSPNC 123
           I   Q+  V GR   D + +  + +   +        + LD EKA+       LI +   
Sbjct: 554 IHPDQSYTVPGRTIFDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQA 613

Query: 124 ESELSRWWDILKLIMAGSGLSINMN 149
            S   ++   LK + A +   + +N
Sbjct: 614 YSFGPQFVGYLKTMYASAECLVKIN 638

BLAST of Lag0039485 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 7.4e-06
Identity = 35/111 (31.53%), Postives = 58/111 (52.25%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKK-KKVDKVNDFRPISPLTSPYKILAKVLAERLKKV 60
           +FQ   + GI+     E  I LIPK  +   K  +FRPIS +    KIL K+LA R+++ 
Sbjct: 495 LFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQH 554

Query: 61  LPHTISDCQAAFVHGRQFLDVILVATEAVEDM-KARKEEGVLVKLDLEKAY 110
           +   I   Q  F+ G Q    I  +   ++ + +A+ +  V++ +D EKA+
Sbjct: 555 IKKLIHHDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAF 605

BLAST of Lag0039485 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 48.5 bits (114), Expect = 1.4e-04
Identity = 34/111 (30.63%), Postives = 56/111 (50.45%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPK-KKKVDKVNDFRPISPLTSPYKILAKVLAERLKKV 60
           +F +    G +     E  I LIPK +K   K+ +FRPIS +    KIL K+LA R+++ 
Sbjct: 502 LFHKIEVEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEH 561

Query: 61  LPHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEG-VLVKLDLEKAY 110
           +   I   Q  F+ G Q    I  +   +  +   K++  +++ LD EKA+
Sbjct: 562 IKAIIHPDQVGFIPGMQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAF 612

BLAST of Lag0039485 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 2.4e-04
Identity = 32/111 (28.83%), Postives = 57/111 (51.35%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKK-KKVDKVNDFRPISPLTSPYKILAKVLAERLKKV 60
           +FQ   + GI+     E  I LIPK  K   +  ++RPIS +    KIL K+L  R+++ 
Sbjct: 494 LFQNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQH 553

Query: 61  LPHTISDCQAAFVHGRQFLDVILVATEAVEDM-KARKEEGVLVKLDLEKAY 110
           +   I   Q  F+ G Q    I  +   ++ + K + ++ +++ +D EKA+
Sbjct: 554 IKKIIHHDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDHMILSIDAEKAF 604

BLAST of Lag0039485 vs. ExPASy TrEMBL
Match: M5WPQ5 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa016677mg PE=4 SV=1)

HSP 1 Score: 223.0 bits (567), Expect = 1.5e-54
Identity = 128/343 (37.32%), Postives = 180/343 (52.48%), Query Frame = 0

Query: 1    MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVL 60
            + Q+FFQ+GIVN  TNET+ICLIPKK    KV D+RPIS +TS YK+++KVLA RL++VL
Sbjct: 746  VMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDYRPISLVTSLYKVISKVLASRLREVL 805

Query: 61   PHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY----------- 120
             +TIS  Q AFV  RQ LD +LVA E VE+++ +K +G++ K+D EKAY           
Sbjct: 806  GNTISQSQGAFVQKRQILDAVLVANEVVEEVRKQKRKGLVFKIDFEKAYDHVEWNFVDDV 865

Query: 121  ------------------------------------------------------------ 180
                                                                        
Sbjct: 866  MARKGFGVKWRGWIIGCLESVNFSIMINGKPRGKFRASRGLRQGDPLSPFLFTLVMEVSH 925

Query: 181  YMYADDTLIFSPNCESELSRWWDILKLIMAGSGLSINMNKTSMISINNDRSMVAQWANSF 240
              +ADDT+      E        +LKL    SG+ IN  K+ ++ IN    ++   A S+
Sbjct: 926  LQFADDTIFLLDGKEEYWLNLLQLLKLFCDVSGMKINKAKSCILGINFSTEVLNNMAGSW 985

Query: 241  GYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKLDMWKRFSLSKGGRVTLAESVLNS 273
            G +    P  YLG PLGGN    +FW+P++EK  ++L  WKR  LSKGGR+TL ++VL+S
Sbjct: 986  GCEVGCWPMIYLGLPLGGNPRALNFWNPVMEKVEKRLQKWKRACLSKGGRLTLIQAVLSS 1045

BLAST of Lag0039485 vs. ExPASy TrEMBL
Match: A0A5E4FWN6 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus dulcis OX=3755 GN=ALMOND_2B035884 PE=4 SV=1)

HSP 1 Score: 221.9 bits (564), Expect = 3.4e-54
Identity = 128/341 (37.54%), Postives = 179/341 (52.49%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVL 60
           + Q+FFQ+GIVN  TNET+ICLIPKK    KV DFRPIS +TS YK+++KVLA RL++VL
Sbjct: 130 VMQDFFQSGIVNGVTNETFICLIPKKANSVKVTDFRPISLVTSLYKVISKVLASRLREVL 189

Query: 61  PHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY----------- 120
            +TIS  Q AFV  RQ LD +LVA E VE+++ +  +G++ K+D EKAY           
Sbjct: 190 GNTISQSQGAFVQKRQILDAVLVANEVVEEVRKQNRKGLVFKIDFEKAYDHVEWNFVDDV 249

Query: 121 -----------------------------------------------------------Y 180
                                                                       
Sbjct: 250 LARKGFGFRASRGLRQGDPLSPFLFTLVSDVLSRIIERAQDVNLVHGIVSGHDQVEVSHL 309

Query: 181 MYADDTLIFSPNCESELSRWWDILKLIMAGSGLSINMNKTSMISINNDRSMVAQWANSFG 240
            +ADDT+ F    E        +LKL    SG+ IN  K+ ++ IN     +   A S+G
Sbjct: 310 QFADDTIFFLDGKEEYWLNLLQMLKLFCDVSGMKINKAKSCILGINFSIETLNNMAGSWG 369

Query: 241 YQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKLDMWKRFSLSKGGRVTLAESVLNSL 272
            +    P  YLG PLGGN    +FW+P+L+K  ++L  WKR  LSKGGR+TL ++VL+S+
Sbjct: 370 CEVGCWPMVYLGLPLGGNPRALNFWNPVLDKVEKRLQRWKRACLSKGGRLTLIQAVLSSI 429

BLAST of Lag0039485 vs. ExPASy TrEMBL
Match: A5C162 (Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VITISV_015915 PE=4 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 4.4e-54
Identity = 118/295 (40.00%), Postives = 180/295 (61.02%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVL 60
           +F EF ++ I+N+ TN ++I L+PKK    +++D+RPIS +TS YKI+AKVLA RL+ VL
Sbjct: 275 VFVEFHRSRIINQSTNASFIVLLPKKSMSKRISDYRPISLITSLYKIIAKVLARRLRGVL 334

Query: 61  PHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY----------- 120
             TI   Q AFV GRQ LD +L+A E V++ +   EEGV+ K+D EKAY           
Sbjct: 335 HKTIHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEGVVFKIDFEKAYDHVSWDFLDHV 394

Query: 121 -----------YMYADDTLIFSPNCESELSRWWDILKLIMAGSGLSINMNKTSMISINND 180
                          DDT+ FS   E ++    ++L +    SGL +N++K+++  IN +
Sbjct: 395 LEMKGFSPRWRKWMRDDTIFFSSTREEDMMTLKNVLLVFGHISGLKVNLDKSNIYGINLE 454

Query: 181 RSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKLDMWKRFSLSKGG 240
           ++ +++ A     ++S  P  YLG PLGGN     FWDP++E+ +R+LD W++  LS GG
Sbjct: 455 QNHLSRLAEMLDCKASGWPILYLGLPLGGNPKACGFWDPVIERISRRLDGWQKTYLSFGG 514

Query: 241 RVTLAESVLNSLPIYYFSLLKSPKGVISKMEKVTRDFVWNE-GSYKRDSNLVKWE 273
           R+TL +S L  +P Y+ SL K P  + +K+E++ RDF+W+  G  KRD +LV W+
Sbjct: 515 RITLIQSCLTHMPCYFLSLFKIPASMAAKIERLQRDFLWSSVGEGKRD-HLVSWD 568

BLAST of Lag0039485 vs. ExPASy TrEMBL
Match: A0A803PZS1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 220.3 bits (560), Expect = 9.8e-54
Identity = 128/328 (39.02%), Postives = 178/328 (54.27%), Query Frame = 0

Query: 1   MFQEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVL 60
           +F+ F + G +    NET+ICLIPK     KV DFRPIS +TS YKI+AK LA RL+ VL
Sbjct: 175 VFRAFEKEGRIEGSINETFICLIPKILNSCKVKDFRPISLITSVYKIVAKTLATRLRGVL 234

Query: 61  PHTISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY----------- 120
             TIS+ Q AFV GRQ LD +L+A E VE+ ++R ++G + K+DLEKAY           
Sbjct: 235 GETISETQLAFVEGRQILDSVLIANETVEEYRSRGKKGFVFKIDLEKAYDCVDWDFLDLV 294

Query: 121 ---------------------------------------------YMYADDTLIFSPNCE 180
                                                          +ADDTL F  + E
Sbjct: 295 LKEKGFGELWRKWIRGCVSSTLFSLLINGRARGKFRGKDNVQISHLQFADDTLFFVKD-E 354

Query: 181 SELSRWWDILKLIMAGSGLSINMNKTSMISINNDRSMVAQWANSFGYQSSSLPFNYLGFP 240
           + L +  +I++     S L +N+NK+ ++ I  +  +VAQ     G +  + P  YLG P
Sbjct: 355 ASLRKLVEIVETFCGVSSLKVNLNKSQLLGICLEEEVVAQNTEVIGCKVGTWPMTYLGMP 414

Query: 241 LGGNYHRKSFWDPMLEKFNRKLDMWKRFSLSKGGRVTLAESVLNSLPIYYFSLLKSPKGV 273
           LGG+  +  FW+P+L+K  ++LD WK   LS+GGR+TL +SVL+SLPIYY SL K+PK V
Sbjct: 415 LGGSPRKDIFWEPVLDKCAKRLDGWKCSFLSRGGRLTLIQSVLSSLPIYYLSLFKAPKIV 474

BLAST of Lag0039485 vs. ExPASy TrEMBL
Match: A0A4Y1R3V4 (VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_008314 PE=4 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 4.9e-53
Identity = 133/366 (36.34%), Postives = 182/366 (49.73%), Query Frame = 0

Query: 4   EFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKILAKVLAERLKKVLPHT 63
           +FF  GI+N  TNET+ICLIPKKK+  KV+DFRPIS +TS YK+++KVLA RL++VL  T
Sbjct: 169 DFFNCGIINAITNETFICLIPKKKESVKVSDFRPISLVTSLYKMVSKVLASRLREVLGST 228

Query: 64  ISDCQAAFVHGRQFLDVILVATEAVEDMKARKEEGVLVKLDLEKAY-------------- 123
           IS  Q+AFV GRQ LD  L+A E VE+ +   + G++ K+DLEKAY              
Sbjct: 229 ISSYQSAFVQGRQILDAALIANEVVEESRRLNKSGMVFKIDLEKAYDHVEWRFVDEVLIR 288

Query: 124 ------------------------------------------------------------ 183
                                                                       
Sbjct: 289 KGFGDRWRSWIRGCLETANFSVMINGRPRGKFRASRGLRQGDPLSPFLFTLVMDVLSRIM 348

Query: 184 -----------------------YMYADDTLIFSPNCESELSRWWDILKLIMAGSGLSIN 243
                                    +ADDT+ F  + E   +    IL+L    SG++IN
Sbjct: 349 EKAQDADEFHGLSPGNGMVEISHLQFADDTIFFIEDKEEYWNNLLQILELFCFVSGMTIN 408

Query: 244 MNKTSMISINNDRSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKL 273
            +K S++ IN D  MV + A ++G      P  YLG PLGGN     FWDP++EK   +L
Sbjct: 409 KSKCSLVGINLDDGMVNEMAGAWGCDVGVWPMLYLGLPLGGNPRAIKFWDPVVEKVENRL 468

BLAST of Lag0039485 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 62.0 bits (149), Expect = 8.6e-10
Identity = 34/116 (29.31%), Postives = 56/116 (48.28%), Query Frame = 0

Query: 156 NNDRSMVAQWANSFGYQSSSLPFNYLGFPLGGNYHRKSFWDPMLEKFNRKLDMWKRFSLS 215
           +ND+   A   +SF + S +LP  YLG PL       S + P++EK   ++  W    LS
Sbjct: 6   DNDK---ADILHSFPFASGALPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLS 65

Query: 216 KGGRVTLAESVLNSLPIYYFSLLKSPKGVISKMEKVTRDFVWNEGSYKRDSNLVKW 272
             GR+ L  SV++SL  ++ S  + P   I +++ +   F+W+          V W
Sbjct: 66  FAGRLQLISSVIHSLTNFWMSAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAW 118

BLAST of Lag0039485 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.4e-04
Identity = 23/46 (50.00%), Postives = 31/46 (67.39%), Query Frame = 0

Query: 3   QEFFQNGIVNKRTNETYICLIPKKKKVDKVNDFRPISPLTSPYKIL 49
           +EFF+ G + KR N T I LIPK   VD+++ FRP+S  T  YKI+
Sbjct: 575 KEFFRTGHLLKRFNATAITLIPKVTGVDQLSMFRPVSCCTVVYKII 620

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
VVA31869.17.0e-5437.54Hypothetical predicted protein, partial [Prunus dulcis][more]
CAN66242.19.1e-5440.00hypothetical protein VITISV_015915 [Vitis vinifera][more]
XP_009335445.11.2e-5335.77PREDICTED: uncharacterized protein LOC103928158 [Pyrus x bretschneideri][more]
BBN69746.11.0e-5236.34VIRB2-interacting protein 2 [Prunus dulcis][more]
VVA21938.11.0e-5236.34Hypothetical predicted protein, partial [Prunus dulcis][more]
Match NameE-valueIdentityDescription
P0C2F61.9e-0930.00Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
P143811.1e-0626.90Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV... [more]
O003707.4e-0631.53LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1[more]
P113691.4e-0430.63LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
P085482.4e-0428.83LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
M5WPQ51.5e-5437.32Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRU... [more]
A0A5E4FWN63.4e-5437.54Reverse transcriptase domain-containing protein (Fragment) OS=Prunus dulcis OX=3... [more]
A5C1624.4e-5440.00Reverse transcriptase domain-containing protein OS=Vitis vinifera OX=29760 GN=VI... [more]
A0A803PZS19.8e-5439.02Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A4Y1R3V44.9e-5336.34VIRB2-interacting protein 2 OS=Prunus dulcis OX=3755 GN=Prudu_008314 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G24255.18.6e-1029.31RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT1G43760.11.4e-0450.00DNAse I-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 23..109
e-value: 4.0E-12
score: 46.1
NoneNo IPR availablePANTHERPTHR33116:SF33OS01G0885550 PROTEINcoord: 17..271
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 17..271
NoneNo IPR availableCDDcd01650RT_nLTR_likecoord: 17..185
e-value: 3.0492E-30
score: 111.229

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0039485.1Lag0039485.1mRNA