Lag0038029 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0038029
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Cytochrome P450
Location	chr2: 11751867 .. 11753935 (-)
RNA-Seq Expression	Lag0038029
Synteny	Lag0038029

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATTGTCATCTTCTTCCTACTCATCACCATCTTCCTCTTTCTCGTTAGCAGAAGAAAGTCCTCCAAAAAGCTTCCTCCAGGGTCGCTTGGAATCCCCTTTATCGGCGAAAGCCTCGGTCTTCTCCGTGCCATGCGCACCAACACTGCAGAAGAATGGCTGCTCAACAGAATTGCAAAGTATGGACCAGTTTCAAAGCTGACTCTGTTCGGAAAGCCTACGGTTTTCATTCATGGACAGGCTGCAAACAAGCTCATCTTCTCCAGCGACGACCATACGATCTCCAACCAGCAAACTAAGTCTCTAAAGATGATTTTGGGGGAACGTAATCTGACAGAACTGAGTGGGGAGGATCACAAGCGTGTGAGAGGTGCACTTGTTTCGTTCTTGAAGCCTGAATCTTTGAGGCAATATGTGGGAGAAATGGATGAGGAAGTCAGGAGACATCTCAAGATGCATTGGCAGGGAAAGAAAGAGGTCACTGTAAGTCAAAATGCCCCTAAATTTTATATCATCTGAGTTGTTCAACAAGGTTGATGAACAAAATATTATATCAACTGAGTTGTTCCATATGTGCAAACAGGTACTGCCATTGATGAGGACTCTGACCTTCAACATTGTATGTTCTCTTCTCTTTGGCCTTGAGCAAGGTACAAGAAGAGAGAGATTGATAGAATGCTTTCAAGTAATGGTAGGAGGAACGTGGTCGATCCCAATAAACTTGCCATTCACGAGGTATAATCGAAGCCGACAGGCAAGTATGAGAATCCAAGAGATGCTAAAGGAACTTTTAGATGAAAAGAGGGTGGAAGTGGAAGAAAAGGGTGCTTCCCCACACCAAGATCTAATTACTTGTTTGCTTAGCATCAGAAACGAAGATAACGAACAAGTATTGACTGAGAAGGAAATTCTGCACAATAGTTTGTTGGTAATGGTTGCTGGATATGATACTTCATCTGTTCTGATTACATTCATGGTGCGGCTTTTGGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGGTTTGCTCGTAAACTATCTATCAAATTGTCTTACTCTGAGCTTATAATCATCTTAGCTTCTAAACATAGAGAAAGTAACCTTTTTTAGTAGAAACCCAAGAAATTTCTGTTTCAACATGAATAAAAGAAGTAAACCCAAATCAGGAAATTGGCATTGGAAAATGAAAAACAGAAACTTGATAATTTGAACTGACCCTTAGATAGTTAGAAACTCACCCCATCCAGATTAAAAGTGGAAAGGAGTTTGTCAATTCACCTACAAATAGCTCAACTAGTTAAGTCATACATCTTTGACCAAAGGCAGAGGTTCGAATCCTCACTCTTTTATGTTGTTGAACTCAAAGAAATGGAAAAGACTTTTATCCCTAAGCATACGAATTAGAGAAGGCGAGTCAAAAGTTACTTACAGGGTTGCTTGTGGTCTATGGTTACAGAACAGGAAGAGATAGCAGGAAGTAAGAAATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTGGAGACCTTGAGACTAGTGGCACCTATATTTGGAGGGTTCAGAAGGGCTCTGAAAGATATTGAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAGTAAGCAAAGTTTTCTTCTTTCTTTTGGTTGAAAAACTCATATGACAATATATATTTATCCTCATGTTATTGGATTTCAGATATTTTGGGCATCACCAATGACCCATTTGGATGGTGCAATATTTGAAGAACCACTAAAGTTTGATCCAAGTAGATTTGAGAACCAGGCATCAATTCCACCTTATTGCTTTGTTGCATTTGGTGGGGGTCCAAGAATTTGTCCAGGCTTTGAGTTTGCAAGGGTTGAAACTCTTGTTACAATCCATTACTTGATCACCATGTTCACATGGGAGCTACTTTCAGATGACCTTTTTATTAGAGATCCAATGCCAATTCCTACCAAAGGATTGCCTGTCAAAATCTTTCCAAGAGAAACTTTTCCAATGACTTGTAGCATGTTTATAAATGGAAATCATGATTAA

mRNA sequence

ATGATTGTCATCTTCTTCCTACTCATCACCATCTTCCTCTTTCTCGTTAGCAGAAGAAAGTCCTCCAAAAAGCTTCCTCCAGGGTCGCTTGGAATCCCCTTTATCGGCGAAAGCCTCGGTCTTCTCCGTGCCATGCGCACCAACACTGCAGAAGAATGGCTGCTCAACAGAATTGCAAAGTATGGACCAGTTTCAAAGCTGACTCTGTTCGGAAAGCCTACGGTTTTCATTCATGGACAGGCTGCAAACAAGCTCATCTTCTCCAGCGACGACCATACGATCTCCAACCAGCAAACTAAGTCTCTAAAGATGATTTTGGGGGAACGTAATCTGACAGAACTGAGTGGGGAGGATCACAAGCGTGTGAGAGGTGCACTTGTTTCGTTCTTGAAGCCTGAATCTTTGAGGCAATATGTGGGAGAAATGGATGAGGAAGTCAGGAGACATCTCAAGATGCATTGGCAGGGAAAGAAAGAGGTCACTGTACTGCCATTGATGAGGACTCTGACCTTCAACATTGTATGTTCTCTTCTCTTTGGCCTTGAGCAAGGTACAAGAAGAGAGAGATTGATAGAATGCTTTCAAGTAATGGTAGGAGGAACGTGGTCGATCCCAATAAACTTGCCATTCACGAGGTATAATCGAAGCCGACAGGCAAGTATGAGAATCCAAGAGATGCTAAAGGAACTTTTAGATGAAAAGAGGGTGGAAGTGGAAGAAAAGGGTGCTTCCCCACACCAAGATCTAATTACTTGTTTGCTTAGCATCAGAAACGAAGATAACGAACAAGTATTGACTGAGAAGGAAATTCTGCACAATAGTTTGTTGGTAATGGTTGCTGGATATGATACTTCATCTGTTCTGATTACATTCATGGTGCGGCTTTTGGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGAACAGGAAGAGATAGCAGGAAGTAAGAAATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTGGAGACCTTGAGACTAGTGGCACCTATATTTGGAGGGTTCAGAAGGGCTCTGAAAGATATTGAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAATATTTTGGGCATCACCAATGACCCATTTGGATGGTGCAATATTTGAAGAACCACTAAAGTTTGATCCAAGTAGATTTGAGAACCAGGCATCAATTCCACCTTATTGCTTTGTTGCATTTGGTGGGGGTCCAAGAATTTGTCCAGGCTTTGAGTTTGCAAGGGTTGAAACTCTTGTTACAATCCATTACTTGATCACCATGTTCACATGGGAGCTACTTTCAGATGACCTTTTTATTAGAGATCCAATGCCAATTCCTACCAAAGGATTGCCTGTCAAAATCTTTCCAAGAGAAACTTTTCCAATGACTTGTAGCATGTTTATAAATGGAAATCATGATTAA

Coding sequence (CDS)

ATGATTGTCATCTTCTTCCTACTCATCACCATCTTCCTCTTTCTCGTTAGCAGAAGAAAGTCCTCCAAAAAGCTTCCTCCAGGGTCGCTTGGAATCCCCTTTATCGGCGAAAGCCTCGGTCTTCTCCGTGCCATGCGCACCAACACTGCAGAAGAATGGCTGCTCAACAGAATTGCAAAGTATGGACCAGTTTCAAAGCTGACTCTGTTCGGAAAGCCTACGGTTTTCATTCATGGACAGGCTGCAAACAAGCTCATCTTCTCCAGCGACGACCATACGATCTCCAACCAGCAAACTAAGTCTCTAAAGATGATTTTGGGGGAACGTAATCTGACAGAACTGAGTGGGGAGGATCACAAGCGTGTGAGAGGTGCACTTGTTTCGTTCTTGAAGCCTGAATCTTTGAGGCAATATGTGGGAGAAATGGATGAGGAAGTCAGGAGACATCTCAAGATGCATTGGCAGGGAAAGAAAGAGGTCACTGTACTGCCATTGATGAGGACTCTGACCTTCAACATTGTATGTTCTCTTCTCTTTGGCCTTGAGCAAGGTACAAGAAGAGAGAGATTGATAGAATGCTTTCAAGTAATGGTAGGAGGAACGTGGTCGATCCCAATAAACTTGCCATTCACGAGGTATAATCGAAGCCGACAGGCAAGTATGAGAATCCAAGAGATGCTAAAGGAACTTTTAGATGAAAAGAGGGTGGAAGTGGAAGAAAAGGGTGCTTCCCCACACCAAGATCTAATTACTTGTTTGCTTAGCATCAGAAACGAAGATAACGAACAAGTATTGACTGAGAAGGAAATTCTGCACAATAGTTTGTTGGTAATGGTTGCTGGATATGATACTTCATCTGTTCTGATTACATTCATGGTGCGGCTTTTGGCTAATAATCCAACTGTTTATGCAGCTGTTCTTCAAGAACAGGAAGAGATAGCAGGAAGTAAGAAATGTGGAGAGTTGCTCACTTGGGAAGATCTTGCCAAGATGAAGTACACATGGAGAGTTGCATTGGAGACCTTGAGACTAGTGGCACCTATATTTGGAGGGTTCAGAAGGGCTCTGAAAGATATTGAATTTGGTGGCTACCTTATTCCTAAAGGATGGCAAATATTTTGGGCATCACCAATGACCCATTTGGATGGTGCAATATTTGAAGAACCACTAAAGTTTGATCCAAGTAGATTTGAGAACCAGGCATCAATTCCACCTTATTGCTTTGTTGCATTTGGTGGGGGTCCAAGAATTTGTCCAGGCTTTGAGTTTGCAAGGGTTGAAACTCTTGTTACAATCCATTACTTGATCACCATGTTCACATGGGAGCTACTTTCAGATGACCTTTTTATTAGAGATCCAATGCCAATTCCTACCAAAGGATTGCCTGTCAAAATCTTTCCAAGAGAAACTTTTCCAATGACTTGTAGCATGTTTATAAATGGAAATCATGATTAA

Protein sequence

MIVIFFLLITIFLFLVSRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRETFPMTCSMFINGNHD

Homology

[Close]

BLAST of Lag0038029 vs. NCBI nr
Match: XP_022139355.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia])

HSP 1 Score: 800.0 bits (2065), Expect = 1.1e-227
Identity = 388/471 (82.38%), Postives = 431/471 (91.51%), Query Frame = 0


Query: 2   IVIFFLLITIFLFLV--SRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIA 61
           + IF  LI IFLFL    R +SS+KLPPGSLG+PFIG+SLGLLRAMR+NTAE+WL  RI 
Sbjct: 5   LTIFSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWLQRRIT 64

Query: 62  KYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDH 121
           KYGP+SKLTLFGKPTV + GQ ANKLIF+ D   ISNQQ +SL+ ILGE NL EL+G+DH
Sbjct: 65  KYGPISKLTLFGKPTVLVGGQGANKLIFNGDT-AISNQQNESLRAILGESNLLELTGDDH 124

Query: 122 KRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLF 181
           KRVR ALV FLKPE L+QYVG+MDEEVRRHL+MHWQ K+EVTVLPLM+TLTFNIVCSLLF
Sbjct: 125 KRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIVCSLLF 184

Query: 182 GLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVE 241
           GLE+GTRRER+IECFQVM+GG WSIPINLPFTRYN+SRQAS++IQEMLK LLDEKRVE+E
Sbjct: 185 GLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEKRVELE 244

Query: 242 EKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANN 301
            KG SPHQDLITCLLSI+N+++EQVLTEKEI+HN +LVMVAGYDTSSVLITFMVR LANN
Sbjct: 245 VKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVRFLANN 304

Query: 302 PTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDI 361
           PTVYAAVLQEQEEIA SK+CGELLTWEDLAKMKYTWRVALETLRLVAP+FGGFR+A+KDI
Sbjct: 305 PTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAMKDI 364

Query: 362 EFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICP 421
           EFGGYLIPKGWQIFWASPMTHLD  IFEEPLKFDP+RFENQAS+P YCFVAFGGGPRICP
Sbjct: 365 EFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPRICP 424

Query: 422 GFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRET 471
           G+EFARVETLVTIHYLIT FTW+LL DDLFIRDPMP+PTKGLP+K+ PRET
Sbjct: 425 GYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRET 474

[Close]

BLAST of Lag0038029 vs. NCBI nr
Match: XP_008457111.1 (PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa] >TYK14034.1 cytochrome P450 716B1-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 769.6 bits (1986), Expect = 1.6e-218
Identity = 379/486 (77.98%), Postives = 423/486 (87.04%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRKSSKK--LPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRI 60
           + ++F LLI IFLFL   R SSKK  LPPGSLG PFIG+SL LLRAMRTNTAE+W   RI
Sbjct: 5   LTILFCLLIPIFLFL---RTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQRI 64

Query: 61  AKYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGED 120
             YG +SKLTLFGKPTVFI GQ+ NKLIFS D   +SNQQ +SL+ ILGERNL EL+GED
Sbjct: 65  RNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGED 124

Query: 121 HKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLL 180
           HKRVR ALVSFLKPE L++YVG+MDEE+R H+ MHW  K+EVTVLPLM+TLTFNIVCSLL
Sbjct: 125 HKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSLL 184

Query: 181 FGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEV 240
           FGLEQG RRER+IECF+VM+ G WSIPINLPFTRYN+SR+AS +IQEMLKELLDEKRVE+
Sbjct: 185 FGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVEL 244

Query: 241 EEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLAN 300
           EEKG S HQDLITCLLSIRNE+NE VL+EKEI+HN +LVMVAG+DTSSVLITFM+R LAN
Sbjct: 245 EEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLAN 304

Query: 301 NPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKD 360
           NPTVYAAVLQEQEEIA SKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+A+ D
Sbjct: 305 NPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMND 364

Query: 361 IEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRIC 420
           I+FGGYLIPKGWQIFW SP+THLD  IF EP KFDP RF+N ASIPPYCF+ FG GPRIC
Sbjct: 365 IQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRIC 424

Query: 421 PGFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRETFPMTCSMF 480
           PG EFARVETLVTIHYLIT FTW+L+SDD FIRDPMP PTKG+P+KI+PR+TF M C M 
Sbjct: 425 PGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTF-MACRMC 484

Query: 481 INGNHD 485
            + NHD
Sbjct: 485 RSENHD 486

[Close]

BLAST of Lag0038029 vs. NCBI nr
Match: XP_038899130.1 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida])

HSP 1 Score: 758.4 bits (1957), Expect = 3.6e-215
Identity = 376/477 (78.83%), Postives = 418/477 (87.63%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAK 60
           +I  F LLI IFLFL++ RKSSK LPPGSLG PFIG+SL LLRAMR NTAEEW   R+ K
Sbjct: 5   LITTFSLLIPIFLFLLTGRKSSKNLPPGSLGFPFIGQSLSLLRAMRINTAEEWAQQRMRK 64

Query: 61  YGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHK 120
           YG +SKLTLFGKPTVF+ GQ+ +KLIFS  D  ISNQQ +SL+ ILGE NL EL GEDHK
Sbjct: 65  YGAISKLTLFGKPTVFVGGQSGHKLIFSG-DCGISNQQNESLRAILGECNLLELIGEDHK 124

Query: 121 RVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFG 180
           RVR ALVSFLKPE L+QYVG+MD  +R+H+KMHWQGK++VTVLPLM+TLTFNIVCSLLFG
Sbjct: 125 RVRNALVSFLKPECLKQYVGKMD-XIRKHIKMHWQGKQQVTVLPLMKTLTFNIVCSLLFG 184

Query: 181 LEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEE 240
           LEQG RRER+IECF+VM+GG WSIPINLPFTRYN+SR+AS RIQEM+KELLDEKRVE+EE
Sbjct: 185 LEQGMRRERMIECFRVMIGGVWSIPINLPFTRYNQSRRASRRIQEMIKELLDEKRVELEE 244

Query: 241 KGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNP 300
           K +S HQDLITCLLSI NE+NE +LTEKEI+HN +LVMVAG+DTSSVLITF++R LANNP
Sbjct: 245 KRSSSHQDLITCLLSIGNEENELILTEKEIVHNIMLVMVAGFDTSSVLITFIMRFLANNP 304

Query: 301 TVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIE 360
           TVY AVLQEQEEIA SKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFR+A+ DIE
Sbjct: 305 TVYVAVLQEQEEIARSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMNDIE 364

Query: 361 FGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFG-------G 420
           FGGYLIPKGWQIFWASP+THLDG IF EPLKFDP+RF+N  SIPPYCFV FG       G
Sbjct: 365 FGGYLIPKGWQIFWASPVTHLDGTIFREPLKFDPNRFDNPTSIPPYCFVGFGGGPRICPG 424

Query: 421 GPRICPGFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRET 471
           GPRICPG EFARVETLVTIHYLIT FTW+LL DD FIRDPMP PTKG+P+KI PRET
Sbjct: 425 GPRICPGNEFARVETLVTIHYLITRFTWKLLLDDHFIRDPMPTPTKGMPIKICPRET 479

[Close]

BLAST of Lag0038029 vs. NCBI nr
Match: XP_004140824.1 (cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010272 [Cucumis sativus])

HSP 1 Score: 753.4 bits (1944), Expect = 1.2e-213
Identity = 370/470 (78.72%), Postives = 412/470 (87.66%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRKSSKK--LPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRI 60
           + + F LLI IFLFL   R+SSKK  LPPGSLG P IG+SL LLRAMRTNTAE+W   RI
Sbjct: 5   LTIFFCLLIPIFLFL---RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQRI 64

Query: 61  AKYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGED 120
             YG +SKLTLFGKPTVFI GQ+ NKLIFS D   +SNQQ +SL+ ILGERNL EL GED
Sbjct: 65  RNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGED 124

Query: 121 HKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLL 180
           HKRVR ALVSFLKPE L++YVG+MDEE+R H++MHW  K+EVTVLPLM+TLTFNIVCSLL
Sbjct: 125 HKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLL 184

Query: 181 FGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEV 240
           FGLEQGTRRER+IECF+VM+ G WSIPINLPFTRYN+SR+AS +IQEMLKELLDEKRVE+
Sbjct: 185 FGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVEL 244

Query: 241 EEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLAN 300
           EEKG S HQDLITCLLSIRNE+NE VL+EKEI+HN +LV+VAG+DTSSVLITFM+R LAN
Sbjct: 245 EEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLAN 304

Query: 301 NPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKD 360
           NPTVYAAVLQEQEEIA SKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+A+ D
Sbjct: 305 NPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMND 364

Query: 361 IEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRIC 420
           I+FGGYLIPKGWQIFW SP+THLD  IF EP KFDP RF+N ASIPPYCF+ FG GPRIC
Sbjct: 365 IQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRIC 424

Query: 421 PGFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPR 469
           PG EFARVETLVTIHYLIT FTW+LL DD FIRDPMP PTKG+P+KI+PR
Sbjct: 425 PGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 471

[Close]

BLAST of Lag0038029 vs. NCBI nr
Match: KAA8523694.1 (hypothetical protein F0562_010117 [Nyssa sinensis])

HSP 1 Score: 724.2 bits (1868), Expect = 7.6e-205
Identity = 336/467 (71.95%), Postives = 415/467 (88.87%), Query Frame = 0


Query: 4   IFFLLITIFLFLVSRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGP 63
           IF  L+ IF  LV RR SS++LPPGSLGIP IG+SL LL+AM+ NTAE WL  RI KYG 
Sbjct: 21  IFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEERIRKYGS 80

Query: 64  VSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVR 123
           +SKL+LFGKPTVFI+GQAANKL+F+ D   I+NQQ +S++MILG+RNL ELSGEDHKRVR
Sbjct: 81  ISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGEDHKRVR 140

Query: 124 GALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQ 183
            ALVSFLKPE L+QYVG+M+EE+R HL+MHW+GK++VTVLPLM+TLTFNI+CSLLFG+++
Sbjct: 141 DALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSLLFGVQR 200

Query: 184 GTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGA 243
           G RR++L+E FQ M+ G WS+P+NLPFTRYNRS +A+ R+Q MLK+L+ EKRVE+E++GA
Sbjct: 201 GARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVELEQQGA 260

Query: 244 SPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVY 303
           S HQDLITCLLSIR +DNE++++EKEI+HN +LVMVAG+DTSSVLI+F++RLL+N+PTVY
Sbjct: 261 SSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLSNDPTVY 320

Query: 304 AAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGG 363
           AAVLQEQEEIA SK CGELLTWEDLAKMKYTWR A+ETLR+  PIFGGFR+ LKDIE+ G
Sbjct: 321 AAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLKDIEYKG 380

Query: 364 YLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGFEF 423
           YLIPKGWQIFWAS MTH+D +IF++P KFDP+RFENQAS+PPYCFVAFGGGPRICPG+EF
Sbjct: 381 YLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRICPGYEF 440

Query: 424 ARVETLVTIHYLITMFTWEL-LSDDLFIRDPMPIPTKGLPVKIFPRE 470
           AR+ETLVT+HYL+T FTW+L  +D+ F RDPMP+PT+GLP++I P++
Sbjct: 441 ARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPKK 487

[Close]

BLAST of Lag0038029 vs. ExPASy Swiss-Prot
Match: Q50EK1 (Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1)

HSP 1 Score: 390.2 bits (1001), Expect = 3.4e-107
Identity = 200/467 (42.83%), Postives = 290/467 (62.10%), Query Frame = 0


Query: 6   FLLITIFLFLVS--RRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGP 65
           F+ +  F +L+S  R K+ + +PPG+ G P IGE+L  L   R     ++  +R  KYG 
Sbjct: 30  FIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGN 89

Query: 66  VSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVR 125
           V   +L G PTV       N+ +FS+++  + N    S+  +     +T + G+D KR+R
Sbjct: 90  VFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLR 149

Query: 126 GALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQ 185
             L++FL+PE+LR++VG +D   +RHL  HW GK EV  LPL++  TF++ C L   +  
Sbjct: 150 RILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVMALPLLKRYTFSLACDLFASINT 209

Query: 186 GTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGA 245
               +RL   F V V G   IPI+LP TRYN+++ A+  I++ L  +++E+++ +E   A
Sbjct: 210 KDDLDRLWLHFMVFVKGVMQIPIDLPGTRYNKTKHAANAIRQQLGSIINERKIALEAGNA 269

Query: 246 SPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVY 305
           SP QDL++ LLS  +E  E  LT+ EI  N LL++ AG+DTSS  +T +++ LA NP  Y
Sbjct: 270 SPEQDLLSFLLSNVDEQGES-LTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCY 329

Query: 306 AAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGG 365
             VL+EQ  IAGSK+ G+LL WEDL +MKY+WRVA E LRL   + G FR+A+K+  + G
Sbjct: 330 EEVLREQLNIAGSKEEGQLLEWEDLQRMKYSWRVAQEALRLFPAVQGSFRKAIKEFIYDG 389

Query: 366 YLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGFEF 425
           + IPKGW++ W    TH     F  P KFDPSRFE +   PPY FV FGGGPR+CPG EF
Sbjct: 390 FTIPKGWKLHWTVNSTHQKSEYFSNPEKFDPSRFEGEGP-PPYTFVPFGGGPRMCPGNEF 449

Query: 426 ARVETLVTIHYLITMFTWELLSD-DLFIRDPMPIPTKGLPVKIFPRE 470
           AR+E L+ +H ++  F W L++  +  I DPMP P  GLP+K+ P +
Sbjct: 450 ARMEILIFLHNIVKNFNWNLVNPLEKVIVDPMPAPVNGLPIKLVPHD 493

[Close]

BLAST of Lag0038029 vs. ExPASy Swiss-Prot
Match: Q50EK0 (Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 1.3e-106
Identity = 200/468 (42.74%), Postives = 290/468 (61.97%), Query Frame = 0


Query: 6   FLLITIFLFLVS--RRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGP 65
           F+ +  F +L+S  R K+ + +PPG+ G P IGE+L  L   R     ++  +R  KYG 
Sbjct: 30  FIGLAFFFYLISNHRTKAWRGIPPGTFGWPLIGETLEFLGCQRKGNPRDFFDSRTQKYGN 89

Query: 66  VSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVR 125
           V   +L G PTV       N+ +FS+++  + N    S+  +     +T + G+D KR+R
Sbjct: 90  VFTTSLVGHPTVVFCSPEGNRFLFSNENKLVVNSWPSSVGNLFRSSLITTV-GDDAKRLR 149

Query: 126 GALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQ 185
             L++FL+PE+LR++VG +D   +RHL  HW GK EVT LPL++  TF++ C L   +  
Sbjct: 150 RILMTFLRPEALREFVGRVDSMTKRHLAEHWIGKDEVTALPLLKRYTFSLACDLFASINN 209

Query: 186 GTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGA 245
                RL   F V V G   IPI+LP TRYN+++ A+  I++ L  +++E+++ +E   A
Sbjct: 210 KDDLGRLWLHFMVFVKGVMQIPIDLPGTRYNKAKHAANAIRQQLGSIINERKIGLEAGNA 269

Query: 246 SPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVY 305
           SP QDL++ LLS  +E  E  LT+ EI  N LL++ AG+DTSS  +T +++ LA NP  Y
Sbjct: 270 SPEQDLLSFLLSNVDEQGES-LTDNEIQDNILLLLYAGHDTSSSTLTVLLKFLAENPHCY 329

Query: 306 AAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGG 365
             VL+EQ +IAGSK+ G+LL WEDL +MKY+WRVA E LRL     G FR+A+K+  + G
Sbjct: 330 EEVLREQLDIAGSKEAGQLLEWEDLQRMKYSWRVAQEALRLFPAAQGSFRKAIKEFIYDG 389

Query: 366 YLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGFEF 425
           + IPKGW+++W    TH     F  P  FDPSRFE +   PPY FV FGGGPR+CPG EF
Sbjct: 390 FTIPKGWKMYWTVNSTHRKSEYFSNPETFDPSRFEGEGP-PPYTFVPFGGGPRMCPGNEF 449

Query: 426 ARVETLVTIHYLITMFTWELLS-DDLFIRDPMPIPTKGLPVKIFPRET 471
           AR+E LV +H ++    W L++  +  I DPMP P  GLP+K+ P ++
Sbjct: 450 ARLEILVFLHNIVKNCKWNLVNPGEKVIVDPMPAPVNGLPIKLVPHDS 494

[Close]

BLAST of Lag0038029 vs. ExPASy Swiss-Prot
Match: A0A0S2II38 (Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=1 SV=1)

HSP 1 Score: 382.5 bits (981), Expect = 7.1e-105
Identity = 198/473 (41.86%), Postives = 297/473 (62.79%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRKSSK--KLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRI 60
           + V+F  L   FLF  S+  S+    LPPG  G P IGES   L        E+++ +R+
Sbjct: 11  LFVLFVSLSFYFLFYKSKSGSTSGLPLPPGKTGWPVIGESFEFLSTGWKGYPEKFIFDRL 70

Query: 61  AKYGP-VSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGE 120
           AKY   V K +LFG+P     G A NK +FS+++  +      S+  +      T  S E
Sbjct: 71  AKYSSNVFKTSLFGQPAAAFCGAACNKFLFSNENKLVQAWWPDSVNKVFPSSTQTS-SKE 130

Query: 121 DHKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSL 180
           +  ++R  L +FLKPE+L++YVG MD+   +H K  W+ KKEV V PL +  TF I C +
Sbjct: 131 EAIKMRKLLPNFLKPEALQRYVGVMDQIATKHFKSGWENKKEVLVFPLAKNYTFWIACKV 190

Query: 181 LFGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVE 240
              +E+ T+  +L+E F  +  G  S+PI+LP T +N + ++S  +++ L  ++ +++ +
Sbjct: 191 FVSVEEPTQVAKLLEPFNAIASGIISVPIDLPGTPFNSAIKSSKIVRDKLMGIIKQRKTD 250

Query: 241 VEEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLA 300
           + E  ASP QD+++ +L + +++N + +TE +I    L +++ G+DT+S   TF+V+ LA
Sbjct: 251 LGEGKASPTQDILSHML-LTSDENGKFMTEGDIADKILGLLIGGHDTASSACTFVVKFLA 310

Query: 301 NNPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALK 360
             P +Y  V +EQ EIA SKK GELL WED+ KMKY+W VA E LRL  P+ G FR AL 
Sbjct: 311 ELPEIYEGVYKEQMEIAKSKKAGELLNWEDIQKMKYSWNVACEVLRLAPPLQGAFREALT 370

Query: 361 DIEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRI 420
           D  + G+ IPKGW+++W++  TH +  +F EPLKFDPSRFE  A  PPY FV FGGGPR+
Sbjct: 371 DFSYNGFSIPKGWKLYWSANSTHRNSEVFPEPLKFDPSRFEG-AGPPPYSFVPFGGGPRM 430

Query: 421 CPGFEFARVETLVTIHYLITMFTWE-LLSDDLFIRDPMPIPTKGLPVKIFPRE 470
           CPG E+AR+E LV +H+++  F WE ++ D+  + +PMPIP KGLPV++FP +
Sbjct: 431 CPGKEYARLEILVFMHHVVKRFKWEKVIPDEKIVVNPMPIPAKGLPVRLFPHK 480

[Close]

BLAST of Lag0038029 vs. ExPASy Swiss-Prot
Match: Q6WG30 (Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2)

HSP 1 Score: 372.1 bits (954), Expect = 9.6e-102
Identity = 185/465 (39.78%), Postives = 294/465 (63.23%), Query Frame = 0


Query: 8   LITIFLFLVSRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGPVSKL 67
           ++ + L   S+R SS KLPPG LGIPFIGES   LRA+R+N+ E++   R+ K+G V K 
Sbjct: 35  ILLLLLLFRSKRHSSLKLPPGKLGIPFIGESFIFLRALRSNSLEQFFDERVKKFGLVFKT 94

Query: 68  TLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVRGALV 127
           +L G PTV + G A N+LI S+++  +          ++GE ++    GEDH  +R AL 
Sbjct: 95  SLIGHPTVVLCGPAGNRLILSNEEKLVQMSWPAQFMKLMGENSVATRRGEDHIVMRSALA 154

Query: 128 SFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQGTRR 187
            F  P +L+ Y+G+M+ E++ H+   W+GK EV VLPL+R L FNI   L F +     +
Sbjct: 155 GFFGPGALQSYIGKMNTEIQSHINEKWKGKDEVNVLPLVRELVFNISAILFFNIYDKQEQ 214

Query: 188 ERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGASPHQ 247
           +RL +  + ++ G++++PI+LP   ++R+ Q   ++ +++  L+ +++ +++   A+  Q
Sbjct: 215 DRLHKLLETILVGSFALPIDLPGFGFHRALQGRAKLNKIMLSLIKKRKEDLQSGSATATQ 274

Query: 248 DLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVYAAVL 307
           DL++ LL+ R +D    LT  EIL N   ++ A YDT++  +  + +LL++NP  Y  V+
Sbjct: 275 DLLSVLLTFR-DDKGTPLTNDEILDNFSSLLHASYDTTTSPMALIFKLLSSNPECYQKVV 334

Query: 308 QEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGGYLIP 367
           QEQ EI  +K+ GE +TW+DL  MKYTW+VA ETLR+  P+FG FR+A+ DI++ GY IP
Sbjct: 335 QEQLEILSNKEEGEEITWKDLKAMKYTWQVAQETLRMFPPVFGTFRKAITDIQYDGYTIP 394

Query: 368 KGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQAS-IPPYCFVAFGGGPRICPGFEFARV 427
           KGW++ W +  TH     F EP KF PSRF+ +   + PY F+ FGGG R C G+EF+++
Sbjct: 395 KGWKLLWTTYSTHPKDLYFNEPEKFMPSRFDQEGKHVAPYTFLPFGGGQRSCVGWEFSKM 454

Query: 428 ETLVTIHYLITMFT--WELLSDDLFIRDPM-PIPTKGLPVKIFPR 469
           E L+ +H+ +  F+    +  D+    DP+ P+P+KG  +K+FPR
Sbjct: 455 EILLFVHHFVKTFSSYTPVDPDEKISGDPLPPLPSKGFSIKLFPR 498

[Close]

BLAST of Lag0038029 vs. ExPASy Swiss-Prot
Match: Q9AXM6 (Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 1.8e-100
Identity = 185/466 (39.70%), Postives = 288/466 (61.80%), Query Frame = 0


Query: 7   LLITIFLFLVSRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGPVSK 66
           L I + L      +SS KLPPG LG P IGE++ LLR +R+ T +++  +R+ K+GPV  
Sbjct: 32  LAIILLLLFRYNHRSSVKLPPGKLGFPLIGETIQLLRTLRSETPQKFFDDRLKKFGPVYM 91

Query: 67  LTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVRGAL 126
            +L G PTV + G A NKL+ S++D  +  +  KS   ++GE ++    GEDH+ +R AL
Sbjct: 92  TSLIGHPTVVLCGPAGNKLVLSNEDKLVEMEGPKSFMKLIGEDSIVAKRGEDHRILRTAL 151

Query: 127 VSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQGTR 186
             FL  ++L+ Y+G M  E+  H    W+GK EV VLPL+R L F+I  +L F +  G +
Sbjct: 152 ARFLGAQALQNYLGRMSSEIGHHFNEKWKGKDEVKVLPLVRGLIFSIASTLFFDVNDGHQ 211

Query: 187 RERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGASPH 246
           +++L    + ++ G+ S+P++ P TRY +  QA +++ E+L  L+  +R ++    AS  
Sbjct: 212 QKQLHHLLETILVGSLSVPLDFPGTRYRKGLQARLKLDEILSSLIKRRRRDLRSGIASDD 271

Query: 247 QDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVYAAV 306
           QDL++ LL+ R+E     LT++ IL N   +  A YDT+   +  + +LL +NP  +  V
Sbjct: 272 QDLLSVLLTFRDEKGNS-LTDQGILDNFSAMFHASYDTTVAPMALIFKLLYSNPEYHEKV 331

Query: 307 LQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGGYLI 366
            QEQ EI G+KK GE ++W+DL  MKYTW+   E+LR+  P+FG FR+A+ DI + GY I
Sbjct: 332 FQEQLEIIGNKKEGEEISWKDLKSMKYTWQAVQESLRMYPPVFGIFRKAITDIHYDGYTI 391

Query: 367 PKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQA-SIPPYCFVAFGGGPRICPGFEFAR 426
           PKGW++  +   THL    F EP +F PSRFE++   + PY +V FGGG R CPG+EF++
Sbjct: 392 PKGWRVLCSPYTTHLREEYFPEPEEFRPSRFEDEGRHVTPYTYVPFGGGLRTCPGWEFSK 451

Query: 427 VETLVTIHYLITMFTWELLSD--DLFIRDPM-PIPTKGLPVKIFPR 469
           +E L+ +H+ +  F+  +  D  +  + DP+ P+P  G  +K+FPR
Sbjct: 452 IEILLFVHHFVKNFSSYIPVDPNEKVLSDPLPPLPANGFSIKLFPR 496

[Close]

BLAST of Lag0038029 vs. ExPASy TrEMBL
Match: A0A6J1CC35 (LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 GN=LOC111010306 PE=3 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 5.3e-228
Identity = 388/471 (82.38%), Postives = 431/471 (91.51%), Query Frame = 0


Query: 2   IVIFFLLITIFLFLV--SRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIA 61
           + IF  LI IFLFL    R +SS+KLPPGSLG+PFIG+SLGLLRAMR+NTAE+WL  RI 
Sbjct: 5   LTIFSFLIPIFLFLTRGRRSRSSQKLPPGSLGVPFIGQSLGLLRAMRSNTAEQWLQRRIT 64

Query: 62  KYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDH 121
           KYGP+SKLTLFGKPTV + GQ ANKLIF+ D   ISNQQ +SL+ ILGE NL EL+G+DH
Sbjct: 65  KYGPISKLTLFGKPTVLVGGQGANKLIFNGDT-AISNQQNESLRAILGESNLLELTGDDH 124

Query: 122 KRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLF 181
           KRVR ALV FLKPE L+QYVG+MDEEVRRHL+MHWQ K+EVTVLPLM+TLTFNIVCSLLF
Sbjct: 125 KRVRSALVXFLKPECLKQYVGKMDEEVRRHLEMHWQXKQEVTVLPLMKTLTFNIVCSLLF 184

Query: 182 GLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVE 241
           GLE+GTRRER+IECFQVM+GG WSIPINLPFTRYN+SRQAS++IQEMLK LLDEKRVE+E
Sbjct: 185 GLEEGTRRERMIECFQVMIGGVWSIPINLPFTRYNQSRQASIKIQEMLKNLLDEKRVELE 244

Query: 242 EKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANN 301
            KG SPHQDLITCLLSI+N+++EQVLTEKEI+HN +LVMVAGYDTSSVLITFMVR LANN
Sbjct: 245 VKGTSPHQDLITCLLSIKNKEHEQVLTEKEIIHNIILVMVAGYDTSSVLITFMVRFLANN 304

Query: 302 PTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDI 361
           PTVYAAVLQEQEEIA SK+CGELLTWEDLAKMKYTWRVALETLRLVAP+FGGFR+A+KDI
Sbjct: 305 PTVYAAVLQEQEEIARSKECGELLTWEDLAKMKYTWRVALETLRLVAPVFGGFRKAMKDI 364

Query: 362 EFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICP 421
           EFGGYLIPKGWQIFWASPMTHLD  IFEEPLKFDP+RFENQAS+P YCFVAFGGGPRICP
Sbjct: 365 EFGGYLIPKGWQIFWASPMTHLDDTIFEEPLKFDPNRFENQASVPXYCFVAFGGGPRICP 424

Query: 422 GFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRET 471
           G+EFARVETLVTIHYLIT FTW+LL DDLFIRDPMP+PTKGLP+K+ PRET
Sbjct: 425 GYEFARVETLVTIHYLITQFTWKLLLDDLFIRDPMPVPTKGLPIKVCPRET 474

[Close]

BLAST of Lag0038029 vs. ExPASy TrEMBL
Match: A0A5A7TZG7 (Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold363G00020 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.7e-219
Identity = 379/486 (77.98%), Postives = 423/486 (87.04%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRKSSKK--LPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRI 60
           + ++F LLI IFLFL   R SSKK  LPPGSLG PFIG+SL LLRAMRTNTAE+W   RI
Sbjct: 5   LTILFCLLIPIFLFL---RTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQRI 64

Query: 61  AKYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGED 120
             YG +SKLTLFGKPTVFI GQ+ NKLIFS D   +SNQQ +SL+ ILGERNL EL+GED
Sbjct: 65  RNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGED 124

Query: 121 HKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLL 180
           HKRVR ALVSFLKPE L++YVG+MDEE+R H+ MHW  K+EVTVLPLM+TLTFNIVCSLL
Sbjct: 125 HKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSLL 184

Query: 181 FGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEV 240
           FGLEQG RRER+IECF+VM+ G WSIPINLPFTRYN+SR+AS +IQEMLKELLDEKRVE+
Sbjct: 185 FGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVEL 244

Query: 241 EEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLAN 300
           EEKG S HQDLITCLLSIRNE+NE VL+EKEI+HN +LVMVAG+DTSSVLITFM+R LAN
Sbjct: 245 EEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLAN 304

Query: 301 NPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKD 360
           NPTVYAAVLQEQEEIA SKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+A+ D
Sbjct: 305 NPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMND 364

Query: 361 IEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRIC 420
           I+FGGYLIPKGWQIFW SP+THLD  IF EP KFDP RF+N ASIPPYCF+ FG GPRIC
Sbjct: 365 IQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRIC 424

Query: 421 PGFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRETFPMTCSMF 480
           PG EFARVETLVTIHYLIT FTW+L+SDD FIRDPMP PTKG+P+KI+PR+TF M C M 
Sbjct: 425 PGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTF-MACRMC 484

Query: 481 INGNHD 485
            + NHD
Sbjct: 485 RSENHD 486

[Close]

BLAST of Lag0038029 vs. ExPASy TrEMBL
Match: A0A1S3C4V0 (cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1)

HSP 1 Score: 769.6 bits (1986), Expect = 7.7e-219
Identity = 379/486 (77.98%), Postives = 423/486 (87.04%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRKSSKK--LPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRI 60
           + ++F LLI IFLFL   R SSKK  LPPGSLG PFIG+SL LLRAMRTNTAE+W   RI
Sbjct: 5   LTILFCLLIPIFLFL---RTSSKKQHLPPGSLGFPFIGQSLSLLRAMRTNTAEQWAQQRI 64

Query: 61  AKYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGED 120
             YG +SKLTLFGKPTVFI GQ+ NKLIFS D   +SNQQ +SL+ ILGERNL EL+GED
Sbjct: 65  RNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELTGED 124

Query: 121 HKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLL 180
           HKRVR ALVSFLKPE L++YVG+MDEE+R H+ MHW  K+EVTVLPLM+TLTFNIVCSLL
Sbjct: 125 HKRVRNALVSFLKPECLKEYVGKMDEEIRSHIHMHWHRKQEVTVLPLMKTLTFNIVCSLL 184

Query: 181 FGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEV 240
           FGLEQG RRER+IECF+VM+ G WSIPINLPFTRYN+SR+AS +IQEMLKELLDEKRVE+
Sbjct: 185 FGLEQGMRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVEL 244

Query: 241 EEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLAN 300
           EEKG S HQDLITCLLSIRNE+NE VL+EKEI+HN +LVMVAG+DTSSVLITFM+R LAN
Sbjct: 245 EEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVMVAGFDTSSVLITFMMRSLAN 304

Query: 301 NPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKD 360
           NPTVYAAVLQEQEEIA SKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+A+ D
Sbjct: 305 NPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMND 364

Query: 361 IEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRIC 420
           I+FGGYLIPKGWQIFW SP+THLD  IF EP KFDP RF+N ASIPPYCF+ FG GPRIC
Sbjct: 365 IQFGGYLIPKGWQIFWTSPVTHLDDNIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRIC 424

Query: 421 PGFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRETFPMTCSMF 480
           PG EFARVETLVTIHYLIT FTW+L+SDD FIRDPMP PTKG+P+KI+PR+TF M C M 
Sbjct: 425 PGNEFARVETLVTIHYLITQFTWKLISDDHFIRDPMPTPTKGMPIKIWPRQTF-MACRMC 484

Query: 481 INGNHD 485
            + NHD
Sbjct: 485 RSENHD 486

[Close]

BLAST of Lag0038029 vs. ExPASy TrEMBL
Match: A0A0A0LA02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 5.7e-214
Identity = 370/470 (78.72%), Postives = 412/470 (87.66%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRKSSKK--LPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRI 60
           + + F LLI IFLFL   R+SSKK  LPPGSLG P IG+SL LLRAMRTNTAE+W   RI
Sbjct: 5   LTIFFCLLIPIFLFL---RRSSKKRQLPPGSLGFPLIGQSLSLLRAMRTNTAEQWAQQRI 64

Query: 61  AKYGPVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGED 120
             YG +SKLTLFGKPTVFI GQ+ NKLIFS D   +SNQQ +SL+ ILGERNL EL GED
Sbjct: 65  RNYGAISKLTLFGKPTVFIGGQSGNKLIFSGDCAVVSNQQNESLRAILGERNLLELIGED 124

Query: 121 HKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLL 180
           HKRVR ALVSFLKPE L++YVG+MDEE+R H++MHW  K+EVTVLPLM+TLTFNIVCSLL
Sbjct: 125 HKRVRNALVSFLKPECLKEYVGKMDEEIRSHIRMHWHCKQEVTVLPLMKTLTFNIVCSLL 184

Query: 181 FGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEV 240
           FGLEQGTRRER+IECF+VM+ G WSIPINLPFTRYN+SR+AS +IQEMLKELLDEKRVE+
Sbjct: 185 FGLEQGTRRERMIECFRVMISGVWSIPINLPFTRYNQSRRASRKIQEMLKELLDEKRVEL 244

Query: 241 EEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLAN 300
           EEKG S HQDLITCLLSIRNE+NE VL+EKEI+HN +LV+VAG+DTSSVLITFM+R LAN
Sbjct: 245 EEKGGSSHQDLITCLLSIRNEENELVLSEKEIVHNIMLVLVAGFDTSSVLITFMMRNLAN 304

Query: 301 NPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKD 360
           NPTVYAAVLQEQEEIA SKKCGELL WEDLAKMKYTWRVALETLRLVAPIFGGFR+A+ D
Sbjct: 305 NPTVYAAVLQEQEEIARSKKCGELLNWEDLAKMKYTWRVALETLRLVAPIFGGFRKAMND 364

Query: 361 IEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRIC 420
           I+FGGYLIPKGWQIFW SP+THLD  IF EP KFDP RF+N ASIPPYCF+ FG GPRIC
Sbjct: 365 IQFGGYLIPKGWQIFWTSPVTHLDDTIFREPSKFDPDRFKNPASIPPYCFLGFGSGPRIC 424

Query: 421 PGFEFARVETLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPR 469
           PG EFARVETLVTIHYLIT FTW+LL DD FIRDPMP PTKG+P+KI+PR
Sbjct: 425 PGNEFARVETLVTIHYLITQFTWKLLLDDHFIRDPMPTPTKGMPIKIWPR 471

[Close]

BLAST of Lag0038029 vs. ExPASy TrEMBL
Match: A0A5J5A0L0 (Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.7e-205
Identity = 336/467 (71.95%), Postives = 415/467 (88.87%), Query Frame = 0


Query: 4   IFFLLITIFLFLVSRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGP 63
           IF  L+ IF  LV RR SS++LPPGSLGIP IG+SL LL+AM+ NTAE WL  RI KYG 
Sbjct: 21  IFLFLLPIFFLLVRRRSSSQRLPPGSLGIPIIGQSLSLLQAMKANTAERWLEERIRKYGS 80

Query: 64  VSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVR 123
           +SKL+LFGKPTVFI+GQAANKL+F+ D   I+NQQ +S++MILG+RNL ELSGEDHKRVR
Sbjct: 81  ISKLSLFGKPTVFIYGQAANKLVFTGDCSVITNQQAQSVRMILGDRNLLELSGEDHKRVR 140

Query: 124 GALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQ 183
            ALVSFLKPE L+QYVG+M+EE+R HL+MHW+GK++VTVLPLM+TLTFNI+CSLLFG+++
Sbjct: 141 DALVSFLKPECLKQYVGKMEEEIREHLEMHWEGKQKVTVLPLMKTLTFNIICSLLFGVQR 200

Query: 184 GTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGA 243
           G RR++L+E FQ M+ G WS+P+NLPFTRYNRS +A+ R+Q MLK+L+ EKRVE+E++GA
Sbjct: 201 GARRDKLVEYFQEMIQGMWSVPVNLPFTRYNRSLRATARVQNMLKDLIREKRVELEQQGA 260

Query: 244 SPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVY 303
           S HQDLITCLLSIR +DNE++++EKEI+HN +LVMVAG+DTSSVLI+F++RLL+N+PTVY
Sbjct: 261 SSHQDLITCLLSIRGKDNEELISEKEIVHNVMLVMVAGHDTSSVLISFIMRLLSNDPTVY 320

Query: 304 AAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGG 363
           AAVLQEQEEIA SK CGELLTWEDLAKMKYTWR A+ETLR+  PIFGGFR+ LKDIE+ G
Sbjct: 321 AAVLQEQEEIAKSKPCGELLTWEDLAKMKYTWRAAMETLRMFPPIFGGFRKTLKDIEYKG 380

Query: 364 YLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGFEF 423
           YLIPKGWQIFWAS MTH+D +IF++P KFDP+RFENQAS+PPYCFVAFGGGPRICPG+EF
Sbjct: 381 YLIPKGWQIFWASSMTHMDSSIFQDPSKFDPTRFENQASVPPYCFVAFGGGPRICPGYEF 440

Query: 424 ARVETLVTIHYLITMFTWEL-LSDDLFIRDPMPIPTKGLPVKIFPRE 470
           AR+ETLVT+HYL+T FTW+L  +D+ F RDPMP+PT+GLP++I P++
Sbjct: 441 ARIETLVTVHYLVTRFTWKLCCTDNFFSRDPMPVPTEGLPIEIVPKK 487

[Close]

BLAST of Lag0038029 vs. TAIR 10
Match: AT5G36110.1 (cytochrome P450, family 716, subfamily A, polypeptide 1 )

HSP 1 Score: 346.3 bits (887), Expect = 4.0e-95
Identity = 182/478 (38.08%), Postives = 288/478 (60.25%), Query Frame = 0


Query: 1   MIVIFFLLITIFLFLVSRRK----SSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLN 60
           MI++F   I + L L+ R+     S   LPPG+ G+P IGES   L A R    E+++ +
Sbjct: 6   MIILFLSSILLSLLLLLRKHLSHFSYPNLPPGNTGLPLIGESFSFLSAGRQGHPEKFITD 65

Query: 61  RIAKYGP----VSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLT 120
           R+ ++      V K  LFG PT  + G + NK +F++++  + +    S+  I      T
Sbjct: 66  RVRRFSSSSSCVFKTHLFGSPTAVVTGASGNKFLFTNENKLVVSWWPDSVNKIFPSSMQT 125

Query: 121 ELSGEDHKRVRGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFN 180
             S E+ +++R  L  F+KPE+LR+YVG MDE  +RH +  W  + +V V PL +  TF+
Sbjct: 126 S-SKEEARKLRMLLSQFMKPEALRRYVGVMDEIAQRHFETEWANQDQVIVFPLTKKFTFS 185

Query: 181 IVCSLLFGLEQGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLD 240
           I C     +E   R  +L E F  +  G +SIPI+LP TR+NR+ +AS  +++ +  ++ 
Sbjct: 186 IACRSFLSMEDPARVRQLEEQFNTVAVGIFSIPIDLPGTRFNRAIKASRLLRKEVSAIVR 245

Query: 241 EKRVEVEEKGASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFM 300
           +++ E++   A    D+++ +L    E  ++ L +K I      +++ G+DT+S++ TF+
Sbjct: 246 QRKEELKAGKALEEHDILSHMLMNIGETKDEDLADKII-----GLLIGGHDTASIVCTFV 305

Query: 301 VRLLANNPTVYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGF 360
           V  LA  P VY  VLQEQ+EI   KK  E L WED+ KM+Y+W VA E +R+V P+ G F
Sbjct: 306 VNYLAEFPHVYQRVLQEQKEILKEKKEKEGLRWEDIEKMRYSWNVACEVMRIVPPLSGTF 365

Query: 361 RRALKDIEFGGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFG 420
           R A+    F G+ IPKGW+++W++  TH++   F EP +F+P+RFE      PY +V FG
Sbjct: 366 REAIDHFSFKGFYIPKGWKLYWSATATHMNPDYFPEPERFEPNRFEGSGP-KPYTYVPFG 425

Query: 421 GGPRICPGFEFARVETLVTIHYLITMFTWELL--SDDLFIRDPMPIPTKGLPVKIFPR 469
           GGPR+CPG E+AR+E L+ +H L+  F WE +  +++  + DP+PIP KGLP++IFP+
Sbjct: 426 GGPRMCPGKEYARLEILIFMHNLVNRFKWEKVFPNENKIVVDPLPIPDKGLPIRIFPQ 476

[Close]

BLAST of Lag0038029 vs. TAIR 10
Match: AT2G42850.1 (cytochrome P450, family 718 )

HSP 1 Score: 323.2 bits (827), Expect = 3.7e-88
Identity = 164/468 (35.04%), Postives = 286/468 (61.11%), Query Frame = 0


Query: 4   IFFLLITIFLFLVSRRKSSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLN-RIAKYG 63
           +FF       F+  + +  KKL PG +G+P+IGE++   +A ++N   E  +N RI K+G
Sbjct: 24  LFFFRKKHHRFITKKIQKKKKLLPGEMGLPWIGETMDFYKAQKSNRVFEDFVNPRIIKHG 83

Query: 64  PVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRV 123
            + K  + G PT+ ++G  AN+LI S++   + +    S   ++G   +    GE H+ +
Sbjct: 84  NIFKTRIMGSPTIVVNGAEANRLILSNEFSLVVSSWPSSSVQLMGMNCIMAKQGEKHRVL 143

Query: 124 RGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLE 183
           RG + + L    L   + ++ + V+ H +  W+GK+E+++    + LTF +V   L+G++
Sbjct: 144 RGIVANSLSYIGLESLIPKLCDTVKFHHETEWRGKEEISLYRSAKVLTFTVVFECLYGIK 203

Query: 184 QGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKG 243
                  ++E F+ ++ G +++P+  P +++ R+++A + I+  L   + EKR E+E++G
Sbjct: 204 V---EIGMLEVFERVLEGVFALPVEFPCSKFARAKKARLEIETFLVGKVREKRREMEKEG 263

Query: 244 A-SPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPT 303
           A  P+  L + L+    E  + V+TE+E++ N +L++ A +DT+S  ++   ++LA +PT
Sbjct: 264 AEKPNTTLFSRLV---EELIKGVITEEEVVDNMVLLVFAAHDTTSYAMSMTFKMLAQHPT 323

Query: 304 VYAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEF 363
               +LQE  +I  +K  GE LT ED+ KMKY+W+V  ET+RL  PIFG FR+A+ DI++
Sbjct: 324 CRDTLLQEHAQIKANKGEGEYLTVEDVKKMKYSWQVVRETMRLSPPIFGSFRKAVADIDY 383

Query: 364 GGYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGF 423
           GGY IPKGW+I W +  TH +  IF++P+ FDP+RF+    I  Y ++ FGGGPR+C G 
Sbjct: 384 GGYTIPKGWKILWTTYGTHYNPEIFQDPMSFDPTRFDK--PIQAYTYLPFGGGPRLCAGH 443

Query: 424 EFARVETLVTIHYLITMFTWELL-SDDLFIRDPMPIPTKGLPVKIFPR 469
           + A++  LV +H+++T F W L+  D+    DP+P P+ G+P+KI P+
Sbjct: 444 QLAKISILVFMHFVVTGFDWSLVYPDETISMDPLPFPSLGMPIKISPK 483

[Close]

BLAST of Lag0038029 vs. TAIR 10
Match: AT5G05690.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 249.2 bits (635), Expect = 6.7e-66
Identity = 149/462 (32.25%), Postives = 246/462 (53.25%), Query Frame = 0


Query: 12  FLFLVSRRKSSKK-LPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGPVSKLTLF 71
           FL L+ R +  +  LPPGSLG+P IGE+  L+ A +T   E ++  R+A+YG V    LF
Sbjct: 17  FLLLLRRTRYRRMGLPPGSLGLPLIGETFQLIGAYKTENPEPFIDERVARYGSVFMTHLF 76

Query: 72  GKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVRGALVSFL 131
           G+PT+F      N+ +  ++          S+  +LG+ +L  + G  HKR+    +SF 
Sbjct: 77  GEPTIFSADPETNRFVLQNEGKLFECSYPASICNLLGKHSLLLMKGSLHKRMHSLTMSFA 136

Query: 132 KPESLRQYVG-EMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQGTRRER 191
               ++ ++  ++D  VR +L   W  +  V ++   + +TF +    L   + G   E 
Sbjct: 137 NSSIIKDHLMLDIDRLVRFNLD-SWSSR--VLLMEEAKKITFELTVKQLMSFDPGEWSES 196

Query: 192 LIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGASPHQDL 251
           L + + +++ G +S+P+ L  T Y ++ QA  ++ E L  ++  KR E EE+GA   +D+
Sbjct: 197 LRKEYLLVIEGFFSLPLPLFSTTYRKAIQARRKVAEALTVVV-MKRREEEEEGAERKKDM 256

Query: 252 ITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVYAAVLQE 311
           +  LL+      +   +++EI+   + ++VAGY+T+S ++T  V+ L   P   A + +E
Sbjct: 257 LAALLAA-----DDGFSDEEIVDFLVALLVAGYETTSTIMTLAVKFLTETPLALAQLKEE 316

Query: 312 QEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGGYLIPKG 371
            E+I   K     L W D   M +T  V  ETLR+   I G FRRA+ D+E  GY IPKG
Sbjct: 317 HEKIRAMKSDSYSLEWSDYKSMPFTQCVVNETLRVANIIGGVFRRAMTDVEIKGYKIPKG 376

Query: 372 WQIFWASPMTHLDGAIFEEPLKFDPSRFENQA--SIPPYCFVAFGGGPRICPGFEFARVE 431
           W++F +    HLD   F++   F+P R+++ +  + P   F  FGGGPR+CPG+E ARV 
Sbjct: 377 WKVFSSFRAVHLDPNHFKDARTFNPWRWQSNSVTTGPSNVFTPFGGGPRLCPGYELARVA 436

Query: 432 TLVTIHYLITMFTWELLSDDLFIRDPMPIPTKGLPVKIFPRE 470
             V +H L+T F+W     D  +  P     K  P+ +  R+
Sbjct: 437 LSVFLHRLVTGFSWVPAEQDKLVFFPTTRTQKRYPIFVKRRD 469

[Close]

BLAST of Lag0038029 vs. TAIR 10
Match: AT4G19230.1 (cytochrome P450, family 707, subfamily A, polypeptide 1 )

HSP 1 Score: 248.8 bits (634), Expect = 8.7e-66
Identity = 150/462 (32.47%), Postives = 248/462 (53.68%), Query Frame = 0


Query: 10  TIFLF----LVSRRK---SSKKLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYG 69
           ++FL+    L+S+R+   S   LPPG++G P++GE+  L     +     +  ++  +YG
Sbjct: 14  SLFLYFLRCLISQRRFGSSKLPLPPGTMGWPYVGETFQLY----SQDPNVFFQSKQKRYG 73

Query: 70  PVSKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRV 129
            V K  + G P V I    A K +  +  H        S + +LG++ +    G+ H ++
Sbjct: 74  SVFKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHAKL 133

Query: 130 RGALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLE 189
           R  ++    PES+R  V +++   +  L+  W+G   +     M+T TFN+    +FG +
Sbjct: 134 RKLVLRAFMPESIRNMVPDIESIAQDSLR-SWEGTM-INTYQEMKTYTFNVALLSIFGKD 193

Query: 190 QGTRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKG 249
           +   RE L  C+ ++  G  S+P+NLP T +++S +A   + ++L  +L E+R     + 
Sbjct: 194 EVLYREDLKRCYYILEKGYNSMPVNLPGTLFHKSMKARKELSQILARILSERR-----QN 253

Query: 250 ASPHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTV 309
            S H DL+   +  + E     LT+++I  N + V+ A  DT++ +++++++ LA NP V
Sbjct: 254 GSSHNDLLGSFMGDKEE-----LTDEQIADNIIGVIFAARDTTASVMSWILKYLAENPNV 313

Query: 310 YAAVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFG 369
             AV +EQ  I   K+ GE LTW D  KM  T RV  ETLR+ + +   FR A++D+E+ 
Sbjct: 314 LEAVTEEQMAIRKDKEEGESLTWGDTKKMPLTSRVIQETLRVASILSFTFREAVEDVEYE 373

Query: 370 GYLIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGFE 429
           GYLIPKGW++       H    IF  P KFDPSRFE   +  P  F+ FG G   CPG E
Sbjct: 374 GYLIPKGWKVLPLFRNIHHSADIFSNPGKFDPSRFE--VAPKPNTFMPFGNGTHSCPGNE 433

Query: 430 FARVETLVTIHYLITMFTWELL-SDDLFIRDPMPIPTKGLPV 464
            A++E  + IH+L T ++W ++ + D     P  +P  GLP+
Sbjct: 434 LAKLEMSIMIHHLTTKYSWSIVGASDGIQYGPFALPQNGLPI 457

[Close]

BLAST of Lag0038029 vs. TAIR 10
Match: AT5G45340.1 (cytochrome P450, family 707, subfamily A, polypeptide 3 )

HSP 1 Score: 248.4 bits (633), Expect = 1.1e-65
Identity = 151/462 (32.68%), Postives = 246/462 (53.25%), Query Frame = 0


Query: 6   FLLITIFLFLVSRRKSSK-KLPPGSLGIPFIGESLGLLRAMRTNTAEEWLLNRIAKYGPV 65
           FL +  F+  V R  S+K  LPPG++G P++GE+  L     +     +   +  +YG V
Sbjct: 16  FLCLLRFIAGVRRSSSTKLPLPPGTMGYPYVGETFQLY----SQDPNVFFAAKQRRYGSV 75

Query: 66  SKLTLFGKPTVFIHGQAANKLIFSSDDHTISNQQTKSLKMILGERNLTELSGEDHKRVRG 125
            K  + G P V I    A K +  +  H        S + +LG++ +    G+ H ++R 
Sbjct: 76  FKTHVLGCPCVMISSPEAAKFVLVTKSHLFKPTFPASKERMLGKQAIFFHQGDYHSKLRK 135

Query: 126 ALVSFLKPESLRQYVGEMDEEVRRHLKMHWQGKKEVTVLPLMRTLTFNIVCSLLFGLEQG 185
            ++    P+++R  V  ++   +  L   W G  ++     M+T TFN+    + G ++ 
Sbjct: 136 LVLRAFMPDAIRNMVPHIESIAQESLN-SWDG-TQLNTYQEMKTYTFNVALISILGKDEV 195

Query: 186 TRRERLIECFQVMVGGTWSIPINLPFTRYNRSRQASMRIQEMLKELLDEKRVEVEEKGAS 245
             RE L  C+ ++  G  S+PINLP T ++++ +A   + ++L  +L ++R     +  S
Sbjct: 196 YYREDLKRCYYILEKGYNSMPINLPGTLFHKAMKARKELAQILANILSKRR-----QNPS 255

Query: 246 PHQDLITCLLSIRNEDNEQVLTEKEILHNSLLVMVAGYDTSSVLITFMVRLLANNPTVYA 305
            H DL+   +     +++  LT+++I  N + V+ A  DT++ ++T++++ LA+NPTV  
Sbjct: 256 SHTDLLGSFM-----EDKAGLTDEQIADNIIGVIFAARDTTASVLTWILKYLADNPTVLE 315

Query: 306 AVLQEQEEIAGSKKCGELLTWEDLAKMKYTWRVALETLRLVAPIFGGFRRALKDIEFGGY 365
           AV +EQ  I   KK GE LTWED  KM  T+RV  ETLR    +   FR A++D+E+ GY
Sbjct: 316 AVTEEQMAIRKDKKEGESLTWEDTKKMPLTYRVIQETLRAATILSFTFREAVEDVEYEGY 375

Query: 366 LIPKGWQIFWASPMTHLDGAIFEEPLKFDPSRFENQASIPPYCFVAFGGGPRICPGFEFA 425
           LIPKGW++       H +  IF +P KFDPSRFE   +  P  F+ FG G   CPG E A
Sbjct: 376 LIPKGWKVLPLFRNIHHNADIFSDPGKFDPSRFE--VAPKPNTFMPFGSGIHSCPGNELA 435

Query: 426 RVETLVTIHYLITMFTWELLS-DDLFIRDPMPIPTKGLPVKI 466
           ++E  V IH+L T + W ++   D     P  +P  GLP+ +
Sbjct: 436 KLEISVLIHHLTTKYRWSIVGPSDGIQYGPFALPQNGLPIAL 459

The following BLAST results are available for this feature:

BLAST of Lag0038029 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022139355.1	1.1e-227	82.38	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Momordica charantia]	[more]
XP_008457111.1	1.6e-218	77.98	PREDICTED: cytochrome P450 716B1-like [Cucumis melo] >KAA0047356.1 cytochrome P4...	[more]
XP_038899130.1	3.6e-215	78.83	LOW QUALITY PROTEIN: cytochrome P450 716B1-like [Benincasa hispida]	[more]
XP_004140824.1	1.2e-213	78.72	cytochrome P450 716B1 [Cucumis sativus] >KGN57442.1 hypothetical protein Csa_010...	[more]
KAA8523694.1	7.6e-205	71.95	hypothetical protein F0562_010117 [Nyssa sinensis]	[more]

BLAST of Lag0038029 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q50EK1	3.4e-107	42.83	Cytochrome P450 716B1 OS=Picea sitchensis OX=3332 GN=CYP716B1 PE=2 SV=1	[more]
Q50EK0	1.3e-106	42.74	Cytochrome P450 716B2 OS=Picea sitchensis OX=3332 GN=CYP716B2 PE=2 SV=1	[more]
A0A0S2II38	7.1e-105	41.86	Beta-amyrin 28-monooxygenase OS=Kalopanax septemlobus OX=228393 GN=CYP716A94 PE=...	[more]
Q6WG30	9.6e-102	39.78	Taxadiene 5-alpha hydroxylase OS=Taxus cuspidata OX=99806 PE=1 SV=2	[more]
Q9AXM6	1.8e-100	39.70	Taxane 10-beta-hydroxylase OS=Taxus cuspidata OX=99806 GN=CYP725A1 PE=1 SV=1	[more]

BLAST of Lag0038029 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CC35	5.3e-228	82.38	LOW QUALITY PROTEIN: cytochrome P450 716B1-like OS=Momordica charantia OX=3673 G...	[more]
A0A5A7TZG7	7.7e-219	77.98	Cytochrome P450 716B1-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3C4V0	7.7e-219	77.98	cytochrome P450 716B1-like OS=Cucumis melo OX=3656 GN=LOC103496862 PE=3 SV=1	[more]
A0A0A0LA02	5.7e-214	78.72	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G186690 PE=3 SV=1	[more]
A0A5J5A0L0	3.7e-205	71.95	Uncharacterized protein OS=Nyssa sinensis OX=561372 GN=F0562_010117 PE=3 SV=1	[more]

BLAST of Lag0038029 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G36110.1	4.0e-95	38.08	cytochrome P450, family 716, subfamily A, polypeptide 1	[more]
AT2G42850.1	3.7e-88	35.04	cytochrome P450, family 718	[more]
AT5G05690.1	6.7e-66	32.25	Cytochrome P450 superfamily protein	[more]
AT4G19230.1	8.7e-66	32.47	cytochrome P450, family 707, subfamily A, polypeptide 1	[more]
AT5G45340.1	1.1e-65	32.68	cytochrome P450, family 707, subfamily A, polypeptide 3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002401	Cytochrome P450, E-class, group I	PRINTS	PR00463	EP450I	coord: 58..77 score: 30.89 coord: 408..418 score: 48.46 coord: 418..441 score: 26.83 coord: 169..187 score: 22.73 coord: 269..286 score: 26.01 coord: 289..315 score: 23.81 coord: 375..399 score: 28.1
IPR001128	Cytochrome P450	PRINTS	PR00385	P450	coord: 280..297 score: 32.53 coord: 418..429 score: 38.11 coord: 409..418 score: 52.91 coord: 336..347 score: 34.88
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 26..446 e-value: 8.9E-68 score: 229.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 16..466 e-value: 4.0E-120 score: 403.6
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 19..468
None	No IPR available	PIRSR	PIRSR000047-1	PIRSR000047-1	coord: 7..448 e-value: 4.7E-10 score: 36.8
None	No IPR available	PANTHER	PTHR24286:SF237	OS07G0520300 PROTEIN	coord: 8..470
None	No IPR available	PANTHER	PTHR24286	CYTOCHROME P450 26	coord: 8..470
IPR017972	Cytochrome P450, conserved site	PROSITE	PS00086	CYTOCHROME_P450	coord: 411..420

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0038029.1	Lag0038029.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016125	sterol metabolic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen