Lag0036987 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0036987
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	low-temperature-induced cysteine proteinase-like
Location	chr2: 2637674 .. 2639754 (+)
RNA-Seq Expression	Lag0036987
Synteny	Lag0036987

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGACCTACACCTACCGATTCTGGCCGGTTATGGGTCTCCTGTTGTTGGTGATGATAACTCTATCATCGGCGATGATCCCCGAGAGGACAGACGAGGAGGTGAGGGCGGTGTACGACGAGTGGCGTGCCAAGCACGGGAAGTTGAGAGGAGAGCGTGAGAATCGATTCGAGATCTTCAAGGACAATCTCAAGTACATCGACCAAATCAACGCCCAAAATTTGCCCTACCGCTTAGGTTTGAACGCCTTCGCAGATGTGACGAACGAGGAGTACCAATCCATGTACTTGAGAGCCAACTTGGCCGTCGGTTCAAGGCGGAACGAGACGAGCAGCAGCCATCAGCCTCGCCTAGGGGTTAATGTGCCCGACTCTATCGACTGGAGGGCGAAAGGAGCCGTGGCTGGCATTAAAGACCAGGGACCATGTGGTAAGAGACCCTCTCTTCTCTGCTTCTTCCATTTTCTAAGACAATATTACACATGTCACATACACAATAATACAAAGATATGACCATTGACAATATGACAGACTGGCACTAGTTCAAGAAATTCATAATATTCTATGAGTAAGTATTTTTATGATGCCATTGTCCATGTAACAAGGTGAGACCAAGTTTTGAAAACTAAAAAAAAAAAAAAAAAGTGTATCATAAAGTCAATTTTAACGTTAGAGAAATAAAAAGATGGATATATATAAATTAACTATTTTTAAGACTAAAATAAATGAAGGGATCAGTTGTTGGGCGGATTATACTGTGCACAAAGGGTAATGAAAACATATATTATTTAGGGTTAGCCTTGTTAGTAATTCCTAAATTATAGTGTGAAGGAATTGAAGGGTAAATTATTGTTGAAATTGATATGAAGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAAGCATAAGCCAGATTGTTACAGGCAAATTGATGACCTTATCAGAACAGGAGCTGGTGAGTTGTGACACTTCTAACCATGGTTGCAATGGAGGCAATCTTAATAGAGCCTTCCTATTCATCATCAACAACCGCGGCGTTGACACTGATACAGATTATCGTTACACCGCCTCTGATGACCCCTGCATCGAGTCCAAGGTCTCTCTATTGCCACACTCAACTATATTAACTTTCCTTTTTGGGTGCGATCGAGAAGCATATATATATATAGATAGATATTTTAATATATCTGATAATTATATCTTTGTGTTTGTGTCTGTCTTCTTCAGAAAAATACTAGGGTTGTTACAATTGATGGTTATGTAGACGTCCTTCATAACAATGAGAATGCACTAAAAGAAGCAGCAGCCAAACAGGTTGTCTCCGTTTACATTGACGCAAGTGGTAGAAGTTTCCAGCTATATCAGTCGGTATAATATTAACTCCATTTCTATGAAGTTTTGGTTTTTATTTTTTATTTACACTTGGAGTTGGAGAGAGGGTCTGATTTGTTGGATGTGTGTATGTGTTTAATTAGGGTATACTCACTGGAGAGTGTGGAACAAATTTGAACCATGCTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCATGGACTACTGGATTGTGAGAAATTCATGGGGCGTGAGTTGGGGTGATAGTGGGTACATCAGGATGCAACGTAATATCGCTTTCCGGCCAGAAGGTCTCTGTGGGATTGCAATGTGGCCTTCCTACCCCATTAAAAACGGACCAAACCCACCAACTGTATGCGATGAATATAACAGTTGTGACGCCGGTGATACTTGTTGCTGCTTGGTCTCGTCCTCAAACTCGTGCCTTGAATGGGGATGCTGCCCCTATGGCTACCCCATCTGCGACGGCCCTCATAGACTCTGTTGGACGGTATATGCATGTGGCTTTCAATTTCCATTTTGGCATACATCTTTGCCTCGACGCCAGTGGTAAATAATGTTTGAAGACTCAACTAATTAATTATATTGTGTTGTGCAGGGACAGGGACAGGGCAAGAATGACCTTTTTGAAGTGAAGGCCATGAGGCGCACTACAGCTGCAGCCAAACCTCTGTGGGCACAGCTTGGAGGAGTTGGAAAGAGTCGTTGTGCTACTGCTTAA

mRNA sequence

ATGGGGACCTACACCTACCGATTCTGGCCGGTTATGGGTCTCCTGTTGTTGGTGATGATAACTCTATCATCGGCGATGATCCCCGAGAGGACAGACGAGGAGGTGAGGGCGGTGTACGACGAGTGGCGTGCCAAGCACGGGAAGTTGAGAGGAGAGCGTGAGAATCGATTCGAGATCTTCAAGGACAATCTCAAGTACATCGACCAAATCAACGCCCAAAATTTGCCCTACCGCTTAGGTTTGAACGCCTTCGCAGATGTGACGAACGAGGAGTACCAATCCATGTACTTGAGAGCCAACTTGGCCGTCGGTTCAAGGCGGAACGAGACGAGCAGCAGCCATCAGCCTCGCCTAGGGGTTAATGTGCCCGACTCTATCGACTGGAGGGCGAAAGGAGCCGTGGCTGGCATTAAAGACCAGGGACCATGTGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAAGCATAAGCCAGATTGTTACAGGCAAATTGATGACCTTATCAGAACAGGAGCTGGTGAGTTGTGACACTTCTAACCATGGTTGCAATGGAGGCAATCTTAATAGAGCCTTCCTATTCATCATCAACAACCGCGGCGTTGACACTGATACAGATTATCGTTACACCGCCTCTGATGACCCCTGCATCGAGTCCAAGAAAAATACTAGGGTTGTTACAATTGATGGTTATGTAGACGTCCTTCATAACAATGAGAATGCACTAAAAGAAGCAGCAGCCAAACAGGTTGTCTCCGTTTACATTGACGCAAGTGGTAGAAGTTTCCAGCTATATCAGTCGGGTATACTCACTGGAGAGTGTGGAACAAATTTGAACCATGCTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCATGGACTACTGGATTGTGAGAAATTCATGGGGCGTGAGTTGGGGTGATAGTGGGTACATCAGGATGCAACGTAATATCGCTTTCCGGCCAGAAGGTCTCTGTGGGATTGCAATGTGGCCTTCCTACCCCATTAAAAACGGACCAAACCCACCAACTGTATGCGATGAATATAACAGTTGTGACGCCGGTGATACTTGTTGCTGCTTGGTCTCGTCCTCAAACTCGTGCCTTGAATGGGGATGCTGCCCCTATGGCTACCCCATCTGCGACGGCCCTCATAGACTCTGTTGGACGGGACAGGGACAGGGCAAGAATGACCTTTTTGAAGTGAAGGCCATGAGGCGCACTACAGCTGCAGCCAAACCTCTGTGGGCACAGCTTGGAGGAGTTGGAAAGAGTCGTTGTGCTACTGCTTAA

Coding sequence (CDS)

ATGGGGACCTACACCTACCGATTCTGGCCGGTTATGGGTCTCCTGTTGTTGGTGATGATAACTCTATCATCGGCGATGATCCCCGAGAGGACAGACGAGGAGGTGAGGGCGGTGTACGACGAGTGGCGTGCCAAGCACGGGAAGTTGAGAGGAGAGCGTGAGAATCGATTCGAGATCTTCAAGGACAATCTCAAGTACATCGACCAAATCAACGCCCAAAATTTGCCCTACCGCTTAGGTTTGAACGCCTTCGCAGATGTGACGAACGAGGAGTACCAATCCATGTACTTGAGAGCCAACTTGGCCGTCGGTTCAAGGCGGAACGAGACGAGCAGCAGCCATCAGCCTCGCCTAGGGGTTAATGTGCCCGACTCTATCGACTGGAGGGCGAAAGGAGCCGTGGCTGGCATTAAAGACCAGGGACCATGTGGGAGTTGCTGGGCGTTCTCAACTATAGCTGCCGTGGAAAGCATAAGCCAGATTGTTACAGGCAAATTGATGACCTTATCAGAACAGGAGCTGGTGAGTTGTGACACTTCTAACCATGGTTGCAATGGAGGCAATCTTAATAGAGCCTTCCTATTCATCATCAACAACCGCGGCGTTGACACTGATACAGATTATCGTTACACCGCCTCTGATGACCCCTGCATCGAGTCCAAGAAAAATACTAGGGTTGTTACAATTGATGGTTATGTAGACGTCCTTCATAACAATGAGAATGCACTAAAAGAAGCAGCAGCCAAACAGGTTGTCTCCGTTTACATTGACGCAAGTGGTAGAAGTTTCCAGCTATATCAGTCGGGTATACTCACTGGAGAGTGTGGAACAAATTTGAACCATGCTGTTAATGTGGTTGGATATGGGTCTGAAGGGGGCATGGACTACTGGATTGTGAGAAATTCATGGGGCGTGAGTTGGGGTGATAGTGGGTACATCAGGATGCAACGTAATATCGCTTTCCGGCCAGAAGGTCTCTGTGGGATTGCAATGTGGCCTTCCTACCCCATTAAAAACGGACCAAACCCACCAACTGTATGCGATGAATATAACAGTTGTGACGCCGGTGATACTTGTTGCTGCTTGGTCTCGTCCTCAAACTCGTGCCTTGAATGGGGATGCTGCCCCTATGGCTACCCCATCTGCGACGGCCCTCATAGACTCTGTTGGACGGGACAGGGACAGGGCAAGAATGACCTTTTTGAAGTGAAGGCCATGAGGCGCACTACAGCTGCAGCCAAACCTCTGTGGGCACAGCTTGGAGGAGTTGGAAAGAGTCGTTGTGCTACTGCTTAA

Protein sequence

MGTYTYRFWPVMGLLLLVMITLSSAMIPERTDEEVRAVYDEWRAKHGKLRGERENRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVSCDTSNHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPNPPTVCDEYNSCDAGDTCCCLVSSSNSCLEWGCCPYGYPICDGPHRLCWTGQGQGKNDLFEVKAMRRTTAAAKPLWAQLGGVGKSRCATA

Homology

[Close]

BLAST of Lag0036987 vs. NCBI nr
Match: XP_022977015.1 (low-temperature-induced cysteine proteinase-like [Cucurbita maxima])

HSP 1 Score: 571.2 bits (1471), Expect = 7.4e-159
Identity = 298/467 (63.81%), Postives = 339/467 (72.59%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM----IPERTDEEVRAVYDEWRAKHGKLRG----ERENR 64
           TYR  P++ LL  +++TL SA+    IPERTDEEV A+YD+WRAKHGKL      E +NR
Sbjct: 3   TYRSSPIVALLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPETQNR 62

Query: 65  FEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQP 124
           F IFKDNLKYID+INAQNLPYRLGLN FAD+TNEEY+S YL A +A GSRRN+TSS +QP
Sbjct: 63  FHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQP 122

Query: 125 RLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVS 184
           RLG  VPDSIDWR KGAVA IKDQG CGSCWAFSTIAAVE I+QIVTG L+ LSEQELV 
Sbjct: 123 RLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVD 182

Query: 185 CDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDV 244
           CD S N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+SKKN RVV+ID Y DV
Sbjct: 183 CDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDV 242

Query: 245 LHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMD 304
             NNE AL++AAAKQVV+V I+  GRSFQLY+SGI TG CGT+L+H VNVVGYGSEGG+D
Sbjct: 243 PVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVD 302

Query: 305 YWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN------------- 364
           YWIVRNSWG SWG+SGYIRMQRN+A  P+GLCGIAM PSYPIKNG N             
Sbjct: 303 YWIVRNSWGASWGESGYIRMQRNVA-SPKGLCGIAMEPSYPIKNGLNPPNPGPTPPSPVE 362

Query: 365 ------PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPI 424
                 PPTVCD+Y  C A +TCCCL   SN+C  WG               CCP+ YP+
Sbjct: 363 PPTPVKPPTVCDQYYDCPAAETCCCLFQFSNTCFVWGCCPLESATCCDDLYSCCPHDYPV 422

Query: 425 CDGPHRLCWTGQGQGKNDLFEVKAMRRTTAAAKPLWAQLGG--VGKS 427
           CD     C     + KND+F VKAMRRT A AKP WA  GG  VGKS
Sbjct: 423 CDVRAGTCL----KSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKS 464

[Close]

BLAST of Lag0036987 vs. NCBI nr
Match: KAG6608453.1 (Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037789.1 Cysteine proteinase RD21A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 569.3 bits (1466), Expect = 2.8e-158
Identity = 293/453 (64.68%), Postives = 336/453 (74.17%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM------IPERTDEEVRAVYDEWRAKHGK----LRGERE 64
           TYR   +M LL  V+ITLSSA+      I ERTDEEV A+YD+WRAKHGK    LRG+R+
Sbjct: 3   TYRSSQIMPLLFFVVITLSSALAFASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRD 62

Query: 65  NRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSH 124
           +RFEIFKDNLKYID+INAQNLPYRLGLN FAD+TN EY+S YL    A GSRRN+TSS +
Sbjct: 63  DRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRY 122

Query: 125 QPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQEL 184
           QPRLG  +PDS+DWR KGAV  +KDQG CGSCWAFSTIAAVE I+Q+VTG L++LSEQEL
Sbjct: 123 QPRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQEL 182

Query: 185 VSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYV 244
           V CDTS N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+ KKN RVV+IDGY 
Sbjct: 183 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 242

Query: 245 DVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGG 304
           DV  N+E AL++AAA QVV+V I+  GRSFQLYQSGI TG+CGTNL+H VNVVGYGSEGG
Sbjct: 243 DVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGG 302

Query: 305 MDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN----------- 364
           +DYWIVRNSWG SWG+ GYIRMQRNI   P GLCGIAM PSYP KNGPN           
Sbjct: 303 VDYWIVRNSWGGSWGEHGYIRMQRNID-SPTGLCGIAMEPSYPTKNGPNPPNPGPTPPSP 362

Query: 365 --PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPICDGP 419
             PP+VCDEY +C A +TCCC+   SN CLEWG               CCP+ YPIC+  
Sbjct: 363 VKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVR 422

[Close]

BLAST of Lag0036987 vs. NCBI nr
Match: KAG6591396.1 (Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7024273.1 Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 569.3 bits (1466), Expect = 2.8e-158
Identity = 298/467 (63.81%), Postives = 338/467 (72.38%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM----IPERTDEEVRAVYDEWRAKHGKLRG----ERENR 64
           TYR  P++ LL  +++TL SA+    IPERTDEEV A+YD+WRAKHGKL      E +NR
Sbjct: 3   TYRSSPIVPLLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQNR 62

Query: 65  FEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQP 124
           F IFKDNLKYID+INAQNLPYRLGLN FAD+TNEEY+S YL A +A GSRRN+TSS +QP
Sbjct: 63  FHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQP 122

Query: 125 RLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVS 184
           RLG  VPDSIDWR KGAVA IKDQG CGSCWAFSTIAAVE I+QIVTG L+ LSEQELV 
Sbjct: 123 RLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVD 182

Query: 185 CDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDV 244
           CD S N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+SKKN RVV+ID Y DV
Sbjct: 183 CDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDV 242

Query: 245 LHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMD 304
             NNE AL++AAAKQVV+V I+  GRSFQLY+SGI TG CGT+L+H VNVVGYGSEGG+D
Sbjct: 243 PVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVD 302

Query: 305 YWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN------------- 364
           YWIVRNSWG SWG+SGYIRMQRN+A  P+GLCGIAM PSYPIK G N             
Sbjct: 303 YWIVRNSWGASWGESGYIRMQRNVA-SPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPVE 362

Query: 365 ------PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPI 424
                 PPTVCDEY  C A +TCCCL   SN+C  WG               CCP+ YP+
Sbjct: 363 PPTPVKPPTVCDEYYDCPAAETCCCLFQFSNTCFVWGCCPLESATCCDDLYSCCPHDYPV 422

Query: 425 CDGPHRLCWTGQGQGKNDLFEVKAMRRTTAAAKPLWAQLGG--VGKS 427
           CD     C     + KND+F VKAMRRT A AKP WA  GG  VGKS
Sbjct: 423 CDVRAGTCL----KSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKS 464

[Close]

BLAST of Lag0036987 vs. NCBI nr
Match: XP_023524911.1 (low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.8 bits (1462), Expect = 8.1e-158
Identity = 292/449 (65.03%), Postives = 334/449 (74.39%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM------IPERTDEEVRAVYDEWRAKHGK----LRGERE 64
           TYR   +M LL  V+ITLSSA+      I ERTDEEV A+YD+WRAKHGK    LRG+R+
Sbjct: 3   TYRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRD 62

Query: 65  NRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSH 124
           +RFEIFKDNLKYID+INAQNLPYRLGLN FAD+TN EY+S YL    A GSRRN+TSS +
Sbjct: 63  DRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRY 122

Query: 125 QPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQEL 184
           QPRLG  +PDS+DWR KGAV  +KDQG CGSCWAFSTIAAVE I+Q+VTG L++LSEQEL
Sbjct: 123 QPRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQEL 182

Query: 185 VSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYV 244
           V CDTS N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+ KKN RVV+IDGY 
Sbjct: 183 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 242

Query: 245 DVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGG 304
           DV  N+E AL++AAA QVV+V I+  GRSFQLYQSGI TG+CGTNL+H VNVVGYGSEGG
Sbjct: 243 DVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGG 302

Query: 305 MDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN----------- 364
           +DYWIVRNSWG SWG+ GYIRMQRNI   P GLCGIAM PSYP KNGPN           
Sbjct: 303 VDYWIVRNSWGGSWGEHGYIRMQRNID-SPTGLCGIAMEPSYPTKNGPNPPNPGPTPPSP 362

Query: 365 --PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWGCCPY--------GYPICDGPHRLCWTG 419
             PP+VCDEY +C A +TCCC+   SN CLEWGCCP          Y  C     +C   
Sbjct: 363 VKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDFPICNVR 422

[Close]

BLAST of Lag0036987 vs. NCBI nr
Match: XP_023534814.1 (low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.8 bits (1462), Expect = 8.1e-158
Identity = 297/469 (63.33%), Postives = 338/469 (72.07%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM----IPERTDEEVRAVYDEWRAKHGKLRG----ERENR 64
           TYR  P++ LL  +++TL SA+    IPERTD+EV A+YD+WRAKHGKL      E +NR
Sbjct: 3   TYRSSPILALLFFLLVTLPSALTSSIIPERTDDEVMALYDQWRAKHGKLHNHLGPESQNR 62

Query: 65  FEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQP 124
           F IFKDNLKYID+INAQNLPYRLGLN FAD+TNEEY+S YL A +A GSRRN+TSS +QP
Sbjct: 63  FHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQP 122

Query: 125 RLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVS 184
           RLG  VPDSIDWR KGAVA IKDQG CGSCWAFSTIAAVE I+QIVTG L+ LSEQELV 
Sbjct: 123 RLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVD 182

Query: 185 CDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDV 244
           CD S N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+SKKN RVV+ID Y DV
Sbjct: 183 CDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDV 242

Query: 245 LHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMD 304
             NNE AL++AAAKQVV+V I+  GRSFQLY+SGI TG CGT+L+H VNVVGYGSEGG+D
Sbjct: 243 PVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVD 302

Query: 305 YWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN------------- 364
           YWIVRNSWG SWG+SGYIRMQRN+A  P+GLCGIAM PSYPIK G N             
Sbjct: 303 YWIVRNSWGASWGESGYIRMQRNVA-SPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPVE 362

Query: 365 ------------PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWGCCPYG--------YPIC 424
                       PPTVCDEY  C A +TCCCL   SN+C  WGCCP          Y  C
Sbjct: 363 PPTPVEPPTPVKPPTVCDEYYDCPAAETCCCLFQFSNTCFVWGCCPLESATCCDDLYSCC 422

Query: 425 DGPHRLCWTGQG---QGKNDLFEVKAMRRTTAAAKPLWAQLGG--VGKS 427
              + +C  G G   + KND+F VKAMRRT A AKP WA  GG  VGKS
Sbjct: 423 PHDYPVCDVGAGTCLKSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKS 470

[Close]

BLAST of Lag0036987 vs. ExPASy Swiss-Prot
Match: P43297 (Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1)

HSP 1 Score: 435.3 bits (1118), Expect = 8.3e-121
Identity = 231/455 (50.77%), Postives = 285/455 (62.64%), Query Frame = 0


Query: 10  PVMGLLLLVMITLSSAM---------------IPERTDEEVRAVYDEWRAKHGKLRG--- 69
           P M +L L M+ +SSA+                  R++ EV ++Y+ W  KHGK +    
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 70  --ERENRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNE 129
             E++ RFEIFKDNL+++D+ N +NL YRLGL  FAD+TN+EY+S YL A +     R  
Sbjct: 66  LVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-R 125

Query: 130 TSSSHQPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTL 189
           TS  ++ R+G  +P+SIDWR KGAVA +KDQG CGSCWAFSTI AVE I+QIVTG L+TL
Sbjct: 126 TSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITL 185

Query: 190 SEQELVSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVT 249
           SEQELV CDTS N GCNGG ++ AF FII N G+DTD DY Y   D  C + +KN +VVT
Sbjct: 186 SEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVT 245

Query: 250 IDGYVDVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGY 309
           ID Y DV   +E +LK+A A Q +S+ I+A GR+FQLY SGI  G CGT L+H V  VGY
Sbjct: 246 IDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGY 305

Query: 310 GSEGGMDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN------ 369
           G+E G DYWIVRNSWG SWG+SGY+RM RNIA    G CGIA+ PSYPIKNG N      
Sbjct: 306 GTENGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGENPPNPGP 365

Query: 370 -------PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWGCCPY--------GYPICDGPHR 420
                  PPT CD Y +C   +TCCCL      C  WGCCP          Y  C   + 
Sbjct: 366 SPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEYP 425

[Close]

BLAST of Lag0036987 vs. ExPASy Swiss-Prot
Match: Q9FMH8 (Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 9.1e-120
Identity = 233/457 (50.98%), Postives = 286/457 (62.58%), Query Frame = 0


Query: 14  LLLLVMITLSSAM---------------IPERTDEEVRAVYDEWRAKHGK-------LRG 73
           +LLL MI +S AM                  R+D EV  +Y+ W  +HGK       L  
Sbjct: 10  ILLLAMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQNGLGA 69

Query: 74  ERENRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETS 133
           E++ RFEIFKDNL++ID+ N +NL Y+LGL  FAD+TNEEY+SMYL A      R  +TS
Sbjct: 70  EKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAKPT--KRVLKTS 129

Query: 134 SSHQPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSE 193
             +Q R+G  +PDS+DWR +GAVA +KDQG CGSCWAFSTI AVE I++IVTG L++LSE
Sbjct: 130 DRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSE 189

Query: 194 QELVSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTID 253
           QELV CDTS N GCNGG ++ AF FII N G+DT+ DY Y A+D  C +++KN +VVTID
Sbjct: 190 QELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTID 249

Query: 254 GYVDVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGS 313
            Y DV  N+E +LK+A A Q +SV I+A GR+FQLY SG+  G CGT L+H V  VGYG+
Sbjct: 250 SYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHGVVAVGYGT 309

Query: 314 EGGMDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN-------- 373
           E G DYWIVRNSWG  WG+SGYI+M RNI   P G CGIAM  SYPIK G N        
Sbjct: 310 ENGKDYWIVRNSWGNRWGESGYIKMARNIE-APTGKCGIAMEASYPIKKGQNPPNPGPSP 369

Query: 374 -----PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPIC 420
                PPT CD+Y SC   +TCCCL      C  WG               CCP+ YP+C
Sbjct: 370 PSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNSSCCPHEYPVC 429

[Close]

BLAST of Lag0036987 vs. ExPASy Swiss-Prot
Match: P25776 (Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=1 SV=2)

HSP 1 Score: 411.8 bits (1057), Expect = 9.8e-114
Identity = 230/448 (51.34%), Postives = 280/448 (62.50%), Query Frame = 0


Query: 14  LLLLVMITLSSAMIPERTDEEVRAVYDEWRAKHGK---LRGERENRFEIFKDNLKYIDQI 73
           LL L    +S     ER++EE R +Y EW+A+HGK     GE E R+  F+DNL+YID+ 
Sbjct: 15  LLSLAAADMSIVSYGERSEEEARRLYAEWKAEHGKSYNAVGEEERRYAAFRDNLRYIDEH 74

Query: 74  NAQN----LPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQPRLGVNVPDSI 133
           NA        +RLGLN FAD+TNEEY+  YL        R  + S  +       +P+S+
Sbjct: 75  NAAADAGVHSFRLGLNRFADLTNEEYRDTYLGLR-NKPRRERKVSDRYLAADNEALPESV 134

Query: 134 DWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVSCDTS-NHGCN 193
           DWR KGAVA IKDQG CGSCWAFS IAAVE I+QIVTG L++LSEQELV CDTS N GCN
Sbjct: 135 DWRTKGAVAEIKDQGGCGSCWAFSAIAAVEGINQIVTGDLISLSEQELVDCDTSYNEGCN 194

Query: 194 GGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDVLHNNENALKE 253
           GG ++ AF FIINN G+DT+ DY Y   D+ C  ++KN +VVTID Y DV  N+E +L++
Sbjct: 195 GGLMDYAFDFIINNGGIDTEDDYPYKGKDERCDVNRKNAKVVTIDSYEDVTPNSETSLQK 254

Query: 254 AAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMDYWIVRNSWGV 313
           A A Q VSV I+A GR+FQLY SGI TG+CGT L+H V  VGYG+E G DYWIVRNSWG 
Sbjct: 255 AVANQPVSVAIEAGGRAFQLYSSGIFTGKCGTALDHGVAAVGYGTENGKDYWIVRNSWGK 314

Query: 314 SWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN-------------PPTVCDEYNS 373
           SWG+SGY+RM+RNI     G CGIA+ PSYP+K G N             PPTVCD Y +
Sbjct: 315 SWGESGYVRMERNIK-ASSGKCGIAVEPSYPLKKGENPPNPGPTPPSPTPPPTVCDNYYT 374

Query: 374 CDAGDTCCCLVSSSNSCLEWGCCPY-GYPICDG-----PHR--LCWTGQG---QGKNDLF 430
           C    TCCC+      C  WGCCP  G   CD      PH   +C   QG     K+   
Sbjct: 375 CPDSTTCCCIYEYGKYCYAWGCCPLEGATCCDDHYSCCPHEYPICNVQQGTCLMAKDSPL 434

[Close]

BLAST of Lag0036987 vs. ExPASy Swiss-Prot
Match: Q9LT78 (Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 2.5e-109
Identity = 213/430 (49.53%), Postives = 281/430 (65.35%), Query Frame = 0


Query: 15  LLLVMITLSSAMIPE--RTDEEVRAVYDEWRAKHGKL---RGERENRFEIFKDNLKYIDQ 74
           +LL+ ++L S    E  R + E R +Y+ W  ++ K     GE+E RFEIFKDNLK++++
Sbjct: 17  VLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEE 76

Query: 75  INA-QNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQPRLGVNVPDSIDW 134
            ++  N  Y +GL  FAD+TN+E++++YLR+ +   +R       +  ++G ++PD+IDW
Sbjct: 77  HSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKME-RTRVPVKGEKYLYKVGDSLPDAIDW 136

Query: 135 RAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVSCDTS-NHGCNGG 194
           RAKGAV  +KDQG CGSCWAFS I AVE I+QI TG+L++LSEQELV CDTS N GC GG
Sbjct: 137 RAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGG 196

Query: 195 NLNRAFLFIINNRGVDTDTDYRYTASD-DPCIESKKNTRVVTIDGYVDVLHNNENALKEA 254
            ++ AF FII N G+DT+ DY Y A+D + C   KKNTRVVTIDGY DV  N+E +LK+A
Sbjct: 197 LMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKA 256

Query: 255 AAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMDYWIVRNSWGVS 314
            A Q +SV I+A GR+FQLY SG+ TG CGT+L+H V  VGYGSEGG DYWIVRNSWG +
Sbjct: 257 LANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSN 316

Query: 315 WGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIK-NGPNP-------PTVCDEYNSCDAGDT 374
           WG+SGY +++RNI     G CG+AM  SYP K +G NP       P VCD+ N+C A  T
Sbjct: 317 WGESGYFKLERNIK-ESSGKCGVAMMASYPTKSSGSNPPKPPAPSPVVCDKSNTCPAKST 376

Query: 375 CCCLVSSSNSCLEWGCCPY---------------GYPICDGPHRLCWTGQGQGKNDLFEV 414
           CCCL   +  C  WGCCPY                YP+CD     C   + +G + L  +
Sbjct: 377 CCCLYEYNGKCYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTC---RMKGNSPL-SI 436

[Close]

BLAST of Lag0036987 vs. ExPASy Swiss-Prot
Match: P25777 (Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1 SV=2)

HSP 1 Score: 382.5 bits (981), Expect = 6.4e-105
Identity = 206/426 (48.36%), Postives = 264/426 (61.97%), Query Frame = 0


Query: 31  TDEEVRAVYDEWRAKHG-----KLRGERENRFEIFKDNLKYIDQINA---QNLPYRLGLN 90
           T+ E RA YD W A++G      L GE E RF +F DNLK++D  NA   +   +RLG+N
Sbjct: 44  TEAEARAAYDLWLAENGGGSPNALGGEHERRFLVFWDNLKFVDAHNARADERGGFRLGMN 103

Query: 91  AFADVTNEEYQSMYLRANLAVGSRRNETSSSHQPRLGV-NVPDSIDWRAKGAVAGIKDQG 150
            FAD+TNEE+++ +L A +A  SR       H    GV  +P+S+DWR KGAVA +K+QG
Sbjct: 104 RFADLTNEEFRATFLGAKVAERSRAAGERYRHD---GVEELPESVDWREKGAVAPVKNQG 163

Query: 151 PCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVSCDTS--NHGCNGGNLNRAFLFIINN 210
            CGSCWAFS ++ VESI+Q+VTG+++TLSEQELV C T+  N GCNGG ++ AF FII N
Sbjct: 164 QCGSCWAFSAVSTVESINQLVTGEMITLSEQELVECSTNGQNSGCNGGLMDDAFDFIIKN 223

Query: 211 RGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDVLHNNENALKEAAAKQVVSVYIDAS 270
            G+DT+ DY Y A D  C  +++N +VV+IDG+ DV  N+E +L++A A Q VSV I+A 
Sbjct: 224 GGIDTEDDYPYKAVDGKCDINRENAKVVSIDGFEDVPQNDEKSLQKAVAHQPVSVAIEAG 283

Query: 271 GRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMDYWIVRNSWGVSWGDSGYIRMQRNI 330
           GR FQLY SG+ +G CGT+L+H V  VGYG++ G DYWIVRNSWG  WG+SGY+RM+RNI
Sbjct: 284 GREFQLYHSGVFSGRCGTSLDHGVVAVGYGTDNGKDYWIVRNSWGPKWGESGYVRMERNI 343

Query: 331 AFRPEGLCGIAMWPSYPIKNGPNPPT-------------------VCDEYNSCDAGDTCC 390
                G CGIAM  SYP K+G NPP                    VCD+  SC AG TCC
Sbjct: 344 NV-TTGKCGIAMMASYPTKSGANPPKPSPTPPTPPTPPPPSAPDHVCDDNFSCPAGSTCC 403

Query: 391 CLVSSSNSCLEWGCCPY---------------GYPICDGPHRLCWTGQGQGKNDLFEVKA 412
           C     N CL WGCCP                 YP+C+     C       KN    VKA
Sbjct: 404 CAFGFRNLCLVWGCCPVEGATCCKDHASCCPPDYPVCNTRAGTC----SASKNSPLSVKA 461

[Close]

BLAST of Lag0036987 vs. ExPASy TrEMBL
Match: A0A6J1IL34 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111477211 PE=3 SV=1)

HSP 1 Score: 571.2 bits (1471), Expect = 3.6e-159
Identity = 298/467 (63.81%), Postives = 339/467 (72.59%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM----IPERTDEEVRAVYDEWRAKHGKLRG----ERENR 64
           TYR  P++ LL  +++TL SA+    IPERTDEEV A+YD+WRAKHGKL      E +NR
Sbjct: 3   TYRSSPIVALLFFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPETQNR 62

Query: 65  FEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQP 124
           F IFKDNLKYID+INAQNLPYRLGLN FAD+TNEEY+S YL A +A GSRRN+TSS +QP
Sbjct: 63  FHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQP 122

Query: 125 RLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVS 184
           RLG  VPDSIDWR KGAVA IKDQG CGSCWAFSTIAAVE I+QIVTG L+ LSEQELV 
Sbjct: 123 RLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVEGINQIVTGDLVALSEQELVD 182

Query: 185 CDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDV 244
           CD S N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+SKKN RVV+ID Y DV
Sbjct: 183 CDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDV 242

Query: 245 LHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMD 304
             NNE AL++AAAKQVV+V I+  GRSFQLY+SGI TG CGT+L+H VNVVGYGSEGG+D
Sbjct: 243 PVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVD 302

Query: 305 YWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN------------- 364
           YWIVRNSWG SWG+SGYIRMQRN+A  P+GLCGIAM PSYPIKNG N             
Sbjct: 303 YWIVRNSWGASWGESGYIRMQRNVA-SPKGLCGIAMEPSYPIKNGLNPPNPGPTPPSPVE 362

Query: 365 ------PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPI 424
                 PPTVCD+Y  C A +TCCCL   SN+C  WG               CCP+ YP+
Sbjct: 363 PPTPVKPPTVCDQYYDCPAAETCCCLFQFSNTCFVWGCCPLESATCCDDLYSCCPHDYPV 422

Query: 425 CDGPHRLCWTGQGQGKNDLFEVKAMRRTTAAAKPLWAQLGG--VGKS 427
           CD     C     + KND+F VKAMRRT A AKP WA  GG  VGKS
Sbjct: 423 CDVRAGTCL----KSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKS 464

[Close]

BLAST of Lag0036987 vs. ExPASy TrEMBL
Match: A0A6J1F9F2 (low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111443285 PE=3 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 3.9e-158
Identity = 298/467 (63.81%), Postives = 339/467 (72.59%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM----IPERTDEEVRAVYDEWRAKHGKLRG----ERENR 64
           T R  P++ LL+ +++TL SA+    IPERTDEEV A+YD+WRAKHGKL      E +NR
Sbjct: 3   TCRSSPIVPLLVFLLVTLPSALPSSIIPERTDEEVMALYDQWRAKHGKLHNHLGPESQNR 62

Query: 65  FEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQP 124
           F IFKDNLKYID+INAQNLPYRLGLN FAD+TNEEY+S YL A +A GSRRN+TSS +QP
Sbjct: 63  FHIFKDNLKYIDEINAQNLPYRLGLNVFADLTNEEYRSKYLGAKIAAGSRRNKTSSRYQP 122

Query: 125 RLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVS 184
           RLG  VPDSIDWR KGAVA IKDQG CGSCWAFSTIAAVESI+QIVTG L+ LSEQELV 
Sbjct: 123 RLGDEVPDSIDWREKGAVAPIKDQGSCGSCWAFSTIAAVESINQIVTGDLVALSEQELVD 182

Query: 185 CDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDV 244
           CD S N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+SKKN RVV+ID Y DV
Sbjct: 183 CDISYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYKGYDSTCIQSKKNARVVSIDDYEDV 242

Query: 245 LHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMD 304
             NNE AL++AAAKQVV+V I+  GRSFQLY+SGI TG CGT+L+H VNVVGYGSEGG+D
Sbjct: 243 PVNNEKALQKAAAKQVVAVAIEGGGRSFQLYRSGIFTGRCGTDLDHGVNVVGYGSEGGVD 302

Query: 305 YWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN------------- 364
           YWIVRNSWG SWG+SGYIRMQRN+A  P+GLCGIAM PSYPIK G N             
Sbjct: 303 YWIVRNSWGASWGESGYIRMQRNVA-SPKGLCGIAMEPSYPIKTGLNPPNPGPTPPSPVE 362

Query: 365 ------PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPI 424
                 PPTVCDEY  C A +TCCCL   SN+C  WG               CCP+ YP+
Sbjct: 363 PPTPVKPPTVCDEYYDCPAAETCCCLFQFSNTCFVWGCCPLESATCCDDLYSCCPHDYPV 422

Query: 425 CDGPHRLCWTGQGQGKNDLFEVKAMRRTTAAAKPLWAQLGG--VGKS 427
           CD     C     + KND+F VKAMRRT A AKP WA  GG  VGKS
Sbjct: 423 CDVRAGTCL----KSKNDVFGVKAMRRTAAVAKPSWALGGGVSVGKS 464

[Close]

BLAST of Lag0036987 vs. ExPASy TrEMBL
Match: A0A6J1FLR7 (low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 GN=LOC111446375 PE=3 SV=1)

HSP 1 Score: 567.0 bits (1460), Expect = 6.7e-158
Identity = 292/453 (64.46%), Postives = 336/453 (74.17%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM------IPERTDEEVRAVYDEWRAKHGK----LRGERE 64
           TYR   +M LL  V+ITLSSA+      I ERTDEEV A+YD+WRAKHGK    LRG+R+
Sbjct: 3   TYRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRD 62

Query: 65  NRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSH 124
           +RFEIFKDNLKYID+INAQNLPYRLGLN FAD+TN EY+S YL    A GSRRN+TSS +
Sbjct: 63  DRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRY 122

Query: 125 QPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQEL 184
           QPRLG  +PDS+DWR KGAV  +KDQG CGSCWAFSTIAAVE I+Q+VTG L++LSEQEL
Sbjct: 123 QPRLGDKLPDSVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQEL 182

Query: 185 VSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYV 244
           V CDTS N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+ KKN RVV+IDGY 
Sbjct: 183 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 242

Query: 245 DVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGG 304
           DV  N+E AL++AAA QVV+V I+  GRSFQLYQSGI TG+CGTNL+H VNVVGYGSEGG
Sbjct: 243 DVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGG 302

Query: 305 MDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN----------- 364
           +DYWIVRNSWG SWG+ GYIRMQRNI  +  GLCGIAM PSYP KNGPN           
Sbjct: 303 VDYWIVRNSWGGSWGEHGYIRMQRNIDSK-TGLCGIAMEPSYPTKNGPNPPNPGPTPPSP 362

Query: 365 --PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPICDGP 419
             PP+VCDEY +C A +TCCC+   SN CLEWG               CCP+ YPIC+  
Sbjct: 363 VKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVR 422

[Close]

BLAST of Lag0036987 vs. ExPASy TrEMBL
Match: A0A6J1J4D3 (low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=LOC111481130 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 8.8e-158
Identity = 292/453 (64.46%), Postives = 336/453 (74.17%), Query Frame = 0


Query: 5   TYRFWPVMGLLLLVMITLSSAM------IPERTDEEVRAVYDEWRAKHGK----LRGERE 64
           TYR   +M LL  V+ITLSSA+      I ERTDEEV A+YD+WRAKHGK    LRG+R+
Sbjct: 3   TYRSSQIMPLLFFVIITLSSALALASSIISERTDEEVMALYDQWRAKHGKLRNGLRGQRD 62

Query: 65  NRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSH 124
           +RFEIFKDNLKYID+INAQNLPYRLGLN FAD+TN EY+S YL    A GSRRN+TSS +
Sbjct: 63  DRFEIFKDNLKYIDEINAQNLPYRLGLNVFADLTNAEYRSKYLGTRFAAGSRRNKTSSRY 122

Query: 125 QPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQEL 184
           QPRLG  +P+S+DWR KGAV  +KDQG CGSCWAFSTIAAVE I+Q+VTG L++LSEQEL
Sbjct: 123 QPRLGDKLPESVDWREKGAVVPVKDQGSCGSCWAFSTIAAVEGINQVVTGDLVSLSEQEL 182

Query: 185 VSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYV 244
           V CDTS N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+ KKN RVV+IDGY 
Sbjct: 183 VDCDTSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNARVVSIDGYE 242

Query: 245 DVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGG 304
           DV  N+E AL++AAA QVV+V I+  GRSFQLYQSGI TG+CGTNL+H VNVVGYGSEGG
Sbjct: 243 DVPVNDEKALQKAAANQVVAVAIEGGGRSFQLYQSGIFTGKCGTNLDHGVNVVGYGSEGG 302

Query: 305 MDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN----------- 364
           +DYWIVRNSWG SWG+ GYIRMQRNIA    GLCGIAM PSYPIK GPN           
Sbjct: 303 VDYWIVRNSWGGSWGEHGYIRMQRNIA-SSTGLCGIAMEPSYPIKKGPNPPNPGPTPPSP 362

Query: 365 --PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPICDGP 419
             PP+VCDEY +C A +TCCC+   SN CLEWG               CCP+ YPIC+  
Sbjct: 363 VKPPSVCDEYYTCPAAETCCCIFEFSNLCLEWGCCPLESATCCDDHYSCCPHDYPICNVR 422

[Close]

BLAST of Lag0036987 vs. ExPASy TrEMBL
Match: A0A6J1C3C7 (low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 GN=LOC111008097 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 3.8e-153
Identity = 290/459 (63.18%), Postives = 330/459 (71.90%), Query Frame = 0


Query: 6   YRFWPVMGLLLLVMITLSSAM----IPERTDEEVRAVYDEWRAKHGK----LRGERENRF 65
           YR  P+M LL  + ITLSSAM    I  RTDEEV A+Y EW AKHGK    L  ERENRF
Sbjct: 4   YRSSPIMLLLFFIAITLSSAMDYSIISGRTDEEVMALYREWCAKHGKQHNGLGAERENRF 63

Query: 66  EIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQPR 125
           EIFKDNLKYID+IN+QNLPYRLGLN FAD+TN+EY+S YL    A GSRRN+TSS + PR
Sbjct: 64  EIFKDNLKYIDEINSQNLPYRLGLNVFADLTNDEYRSKYLGPKRAAGSRRNKTSSRYHPR 123

Query: 126 LGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVSC 185
           LG N+PDSIDWR KGAV G+KDQG CGSCWAFSTIAAVE I+QIVTG L++LSEQELV C
Sbjct: 124 LGDNLPDSIDWREKGAVVGVKDQGSCGSCWAFSTIAAVEGINQIVTGDLISLSEQELVDC 183

Query: 186 DTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTIDGYVDVL 245
           D S N GCNGG ++ AF FIINN G+DT+ DY Y   D  CI+ KKN +VV+ID Y DV 
Sbjct: 184 DNSYNEGCNGGLMDYAFEFIINNGGIDTEEDYPYNGFDSSCIQYKKNAKVVSIDDYEDVP 243

Query: 246 HNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMDY 305
            NNE AL++A A QVV+V I+  GRS+QLY+SGI TG CGT L+H VNVVGYGSEGG+DY
Sbjct: 244 VNNEKALQKAVANQVVAVAIEGGGRSYQLYESGIFTGRCGTALDHGVNVVGYGSEGGVDY 303

Query: 306 WIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN-------------P 365
           WIVRNSWG SWG+ GYIRMQRN+  +  GLCGIAM PSYPIKNGPN             P
Sbjct: 304 WIVRNSWGKSWGEKGYIRMQRNVPSK-NGLCGIAMEPSYPIKNGPNPPNPGPTPPSPVKP 363

Query: 366 PTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPICDGPHRL 425
           P+VCDEY SC A +TCCC+   SN C+EWG               CCP+ YPIC+     
Sbjct: 364 PSVCDEYYSCPASETCCCIFEFSNLCVEWGCCPLESATCCDDHYSCCPHDYPICNVRAGT 423

Query: 426 CWTGQGQGKNDLFEVKAMRRTTAAAKPLWA-QLGGVGKS 427
           C     +GKND F VKAMRRT AAAKP WA     VGKS
Sbjct: 424 CL----RGKNDPFGVKAMRRTAAAAKPSWAFGDASVGKS 457

[Close]

BLAST of Lag0036987 vs. TAIR 10
Match: AT1G47128.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 435.3 bits (1118), Expect = 5.9e-122
Identity = 231/455 (50.77%), Postives = 285/455 (62.64%), Query Frame = 0


Query: 10  PVMGLLLLVMITLSSAM---------------IPERTDEEVRAVYDEWRAKHGKLRG--- 69
           P M +L L M+ +SSA+                  R++ EV ++Y+ W  KHGK +    
Sbjct: 6   PTMAILFLAMVAVSSAVDMSIISYDEKHGVSTTGGRSEAEVMSIYEAWLVKHGKAQSQNS 65

Query: 70  --ERENRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNE 129
             E++ RFEIFKDNL+++D+ N +NL YRLGL  FAD+TN+EY+S YL A +     R  
Sbjct: 66  LVEKDRRFEIFKDNLRFVDEHNEKNLSYRLGLTRFADLTNDEYRSKYLGAKMEKKGER-R 125

Query: 130 TSSSHQPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTL 189
           TS  ++ R+G  +P+SIDWR KGAVA +KDQG CGSCWAFSTI AVE I+QIVTG L+TL
Sbjct: 126 TSLRYEARVGDELPESIDWRKKGAVAEVKDQGGCGSCWAFSTIGAVEGINQIVTGDLITL 185

Query: 190 SEQELVSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVT 249
           SEQELV CDTS N GCNGG ++ AF FII N G+DTD DY Y   D  C + +KN +VVT
Sbjct: 186 SEQELVDCDTSYNEGCNGGLMDYAFEFIIKNGGIDTDKDYPYKGVDGTCDQIRKNAKVVT 245

Query: 250 IDGYVDVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGY 309
           ID Y DV   +E +LK+A A Q +S+ I+A GR+FQLY SGI  G CGT L+H V  VGY
Sbjct: 246 IDSYEDVPTYSEESLKKAVAHQPISIAIEAGGRAFQLYDSGIFDGSCGTQLDHGVVAVGY 305

Query: 310 GSEGGMDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN------ 369
           G+E G DYWIVRNSWG SWG+SGY+RM RNIA    G CGIA+ PSYPIKNG N      
Sbjct: 306 GTENGKDYWIVRNSWGKSWGESGYLRMARNIA-SSSGKCGIAIEPSYPIKNGENPPNPGP 365

Query: 370 -------PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWGCCPY--------GYPICDGPHR 420
                  PPT CD Y +C   +TCCCL      C  WGCCP          Y  C   + 
Sbjct: 366 SPPSPIKPPTQCDSYYTCPESNTCCCLFEYGKYCFAWGCCPLEAATCCDDNYSCCPHEYP 425

[Close]

BLAST of Lag0036987 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 431.8 bits (1109), Expect = 6.5e-121
Identity = 233/457 (50.98%), Postives = 286/457 (62.58%), Query Frame = 0


Query: 14  LLLLVMITLSSAM---------------IPERTDEEVRAVYDEWRAKHGK-------LRG 73
           +LLL MI +S AM                  R+D EV  +Y+ W  +HGK       L  
Sbjct: 10  ILLLAMIGVSYAMDMSIISYDENHHITTETSRSDSEVERIYEAWMVEHGKKKMNQNGLGA 69

Query: 74  ERENRFEIFKDNLKYIDQINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETS 133
           E++ RFEIFKDNL++ID+ N +NL Y+LGL  FAD+TNEEY+SMYL A      R  +TS
Sbjct: 70  EKDQRFEIFKDNLRFIDEHNTKNLSYKLGLTRFADLTNEEYRSMYLGAKPT--KRVLKTS 129

Query: 134 SSHQPRLGVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSE 193
             +Q R+G  +PDS+DWR +GAVA +KDQG CGSCWAFSTI AVE I++IVTG L++LSE
Sbjct: 130 DRYQARVGDALPDSVDWRKEGAVADVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSE 189

Query: 194 QELVSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRVVTID 253
           QELV CDTS N GCNGG ++ AF FII N G+DT+ DY Y A+D  C +++KN +VVTID
Sbjct: 190 QELVDCDTSYNQGCNGGLMDYAFEFIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTID 249

Query: 254 GYVDVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGS 313
            Y DV  N+E +LK+A A Q +SV I+A GR+FQLY SG+  G CGT L+H V  VGYG+
Sbjct: 250 SYEDVPENSEASLKKALAHQPISVAIEAGGRAFQLYSSGVFDGLCGTELDHGVVAVGYGT 309

Query: 314 EGGMDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPN-------- 373
           E G DYWIVRNSWG  WG+SGYI+M RNI   P G CGIAM  SYPIK G N        
Sbjct: 310 ENGKDYWIVRNSWGNRWGESGYIKMARNIE-APTGKCGIAMEASYPIKKGQNPPNPGPSP 369

Query: 374 -----PPTVCDEYNSCDAGDTCCCLVSSSNSCLEWG---------------CCPYGYPIC 420
                PPT CD+Y SC   +TCCCL      C  WG               CCP+ YP+C
Sbjct: 370 PSPIKPPTTCDKYFSCPESNTCCCLYKYGKYCFGWGCCPLEAATCCDDNSSCCPHEYPVC 429

[Close]

BLAST of Lag0036987 vs. TAIR 10
Match: AT3G19390.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 397.1 bits (1019), Expect = 1.8e-110
Identity = 213/430 (49.53%), Postives = 281/430 (65.35%), Query Frame = 0


Query: 15  LLLVMITLSSAMIPE--RTDEEVRAVYDEWRAKHGKL---RGERENRFEIFKDNLKYIDQ 74
           +LL+ ++L S    E  R + E R +Y+ W  ++ K     GE+E RFEIFKDNLK++++
Sbjct: 17  VLLISLSLGSVTATETTRNEAEARRMYERWLVENRKNYNGLGEKERRFEIFKDNLKFVEE 76

Query: 75  INA-QNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETSSSHQPRLGVNVPDSIDW 134
            ++  N  Y +GL  FAD+TN+E++++YLR+ +   +R       +  ++G ++PD+IDW
Sbjct: 77  HSSIPNRTYEVGLTRFADLTNDEFRAIYLRSKME-RTRVPVKGEKYLYKVGDSLPDAIDW 136

Query: 135 RAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLMTLSEQELVSCDTS-NHGCNGG 194
           RAKGAV  +KDQG CGSCWAFS I AVE I+QI TG+L++LSEQELV CDTS N GC GG
Sbjct: 137 RAKGAVNPVKDQGSCGSCWAFSAIGAVEGINQIKTGELISLSEQELVDCDTSYNDGCGGG 196

Query: 195 NLNRAFLFIINNRGVDTDTDYRYTASD-DPCIESKKNTRVVTIDGYVDVLHNNENALKEA 254
            ++ AF FII N G+DT+ DY Y A+D + C   KKNTRVVTIDGY DV  N+E +LK+A
Sbjct: 197 LMDYAFKFIIENGGIDTEEDYPYIATDVNVCNSDKKNTRVVTIDGYEDVPQNDEKSLKKA 256

Query: 255 AAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVVGYGSEGGMDYWIVRNSWGVS 314
            A Q +SV I+A GR+FQLY SG+ TG CGT+L+H V  VGYGSEGG DYWIVRNSWG +
Sbjct: 257 LANQPISVAIEAGGRAFQLYTSGVFTGTCGTSLDHGVVAVGYGSEGGQDYWIVRNSWGSN 316

Query: 315 WGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIK-NGPNP-------PTVCDEYNSCDAGDT 374
           WG+SGY +++RNI     G CG+AM  SYP K +G NP       P VCD+ N+C A  T
Sbjct: 317 WGESGYFKLERNIK-ESSGKCGVAMMASYPTKSSGSNPPKPPAPSPVVCDKSNTCPAKST 376

Query: 375 CCCLVSSSNSCLEWGCCPY---------------GYPICDGPHRLCWTGQGQGKNDLFEV 414
           CCCL   +  C  WGCCPY                YP+CD     C   + +G + L  +
Sbjct: 377 CCCLYEYNGKCYSWGCCPYESATCCDDGSSCCPQSYPVCDLKANTC---RMKGNSPL-SI 436

[Close]

BLAST of Lag0036987 vs. TAIR 10
Match: AT1G09850.1 (xylem bark cysteine peptidase 3 )

HSP 1 Score: 352.1 bits (902), Expect = 6.5e-97
Identity = 185/387 (47.80%), Postives = 239/387 (61.76%), Query Frame = 0


Query: 31  TDEEVRAVYDEWRAKHGKLRG---ERENRFEIFKDNLKYIDQIN-AQNLPYRLGLNAFAD 90
           + +++  ++D+W  KHGK  G   ER+ R +IFKDN  ++ Q N   N  Y L LNAFAD
Sbjct: 24  SSDDISELFDDWCQKHGKTYGSEEERQQRIQIFKDNHDFVTQHNLITNATYSLSLNAFAD 83

Query: 91  VTNEEYQSMYLRANLAVGSRRNETSSSHQPRLG-VNVPDSIDWRAKGAVAGIKDQGPCGS 150
           +T+ E+++   R  L+V +     +S  Q   G V VPDS+DWR KGAV  +KDQG CG+
Sbjct: 84  LTHHEFKAS--RLGLSVSAPSVIMASKGQSLGGSVKVPDSVDWRKKGAVTNVKDQGSCGA 143

Query: 151 CWAFSTIAAVESISQIVTGKLMTLSEQELVSCDTS-NHGCNGGNLNRAFLFIINNRGVDT 210
           CW+FS   A+E I+QIVTG L++LSEQEL+ CD S N GCNGG ++ AF F+I N G+DT
Sbjct: 144 CWSFSATGAMEGINQIVTGDLISLSEQELIDCDKSYNAGCNGGLMDYAFEFVIKNHGIDT 203

Query: 211 DTDYRYTASDDPCIESKKNTRVVTIDGYVDVLHNNENALKEAAAKQVVSVYIDASGRSFQ 270
           + DY Y   D  C + K   +VVTID Y  V  N+E AL EA A Q VSV I  S R+FQ
Sbjct: 204 EKDYPYQERDGTCKKDKLKQKVVTIDSYAGVKSNDEKALMEAVAAQPVSVGICGSERAFQ 263

Query: 271 LYQSGILTGECGTNLNHAVNVVGYGSEGGMDYWIVRNSWGVSWGDSGYIRMQRNIAFRPE 330
           LY SGI +G C T+L+HAV +VGYGS+ G+DYWIV+NSWG SWG  G++ MQRN     +
Sbjct: 264 LYSSGIFSGPCSTSLDHAVLIVGYGSQNGVDYWIVKNSWGKSWGMDGFMHMQRNTE-NSD 323

Query: 331 GLCGIAMWPSYPIKNGPNP-------PTVCDEYNSCDAGDTCCCLVSSSNSCLEW----- 390
           G+CGI M  SYPIK  PNP       PT C+ +  C +G+TCCC       C  W     
Sbjct: 324 GVCGINMLASYPIKTHPNPPPPSPPGPTKCNLFTYCSSGETCCCARELFGLCFSWKCCEI 383

[Close]

BLAST of Lag0036987 vs. TAIR 10
Match: AT4G36880.1 (cysteine proteinase1 )

HSP 1 Score: 351.7 bits (901), Expect = 8.5e-97
Identity = 188/357 (52.66%), Postives = 239/357 (66.95%), Query Frame = 0


Query: 14  LLLLVMITLSSA---------MIPE----RTDEEVRAVYDEWRAKHGK-------LRGER 73
           LLL V+++L+S           +P     RTDEEVR++Y +W A+HGK       +  ++
Sbjct: 11  LLLYVVVSLASGDESIINDHLQLPSDGKWRTDEEVRSIYLQWSAEHGKTNNNNNGIINDQ 70

Query: 74  ENRFEIFKDNLKYID--QINAQNLPYRLGLNAFADVTNEEYQSMYLRANLAVGSRRNETS 133
           + RF IFKDNL++ID    N +N  Y+LGL  F D+TN+EY+ +YL A      R  +  
Sbjct: 71  DKRFNIFKDNLRFIDLHNENNKNATYKLGLTKFTDLTNDEYRKLYLGARTEPARRIAKAK 130

Query: 134 SSHQPRL----GVNVPDSIDWRAKGAVAGIKDQGPCGSCWAFSTIAAVESISQIVTGKLM 193
           + +Q       G  VP+++DWR KGAV  IKDQG CGSCWAFST AAVE I++IVTG+L+
Sbjct: 131 NVNQKYSAAVNGKEVPETVDWRQKGAVNPIKDQGTCGSCWAFSTTAAVEGINKIVTGELI 190

Query: 194 TLSEQELVSCDTS-NHGCNGGNLNRAFLFIINNRGVDTDTDYRYTASDDPCIESKKNTRV 253
           +LSEQELV CD S N GCNGG ++ AF FI+ N G++T+ DY Y      C    KN+RV
Sbjct: 191 SLSEQELVDCDKSYNQGCNGGLMDYAFQFIMKNGGLNTEKDYPYRGFGGKCNSFLKNSRV 250

Query: 254 VTIDGYVDVLHNNENALKEAAAKQVVSVYIDASGRSFQLYQSGILTGECGTNLNHAVNVV 313
           V+IDGY DV   +E ALK+A + Q VSV I+A GR FQ YQSGI TG CGTNL+HAV  V
Sbjct: 251 VSIDGYEDVPTKDETALKKAISYQPVSVAIEAGGRIFQHYQSGIFTGSCGTNLDHAVVAV 310

Query: 314 GYGSEGGMDYWIVRNSWGVSWGDSGYIRMQRNIAFRPEGLCGIAMWPSYPIKNGPNP 344
           GYGSE G+DYWIVRNSWG  WG+ GYIRM+RN+A    G CGIA+  SYP+K  PNP
Sbjct: 311 GYGSENGVDYWIVRNSWGPRWGEEGYIRMERNLAASKSGKCGIAVEASYPVKYSPNP 367

The following BLAST results are available for this feature:

BLAST of Lag0036987 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022977015.1	7.4e-159	63.81	low-temperature-induced cysteine proteinase-like [Cucurbita maxima]	[more]
KAG6608453.1	2.8e-158	64.68	Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7...	[more]
KAG6591396.1	2.8e-158	63.81	Cysteine proteinase RD21A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7...	[more]
XP_023524911.1	8.1e-158	65.03	low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo]	[more]
XP_023534814.1	8.1e-158	63.33	low-temperature-induced cysteine proteinase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0036987 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43297	8.3e-121	50.77	Cysteine proteinase RD21A OS=Arabidopsis thaliana OX=3702 GN=RD21A PE=1 SV=1	[more]
Q9FMH8	9.1e-120	50.98	Probable cysteine protease RD21B OS=Arabidopsis thaliana OX=3702 GN=RD21B PE=1 S...	[more]
P25776	9.8e-114	51.34	Oryzain alpha chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0650000 PE=...	[more]
Q9LT78	2.5e-109	49.53	Probable cysteine protease RD21C OS=Arabidopsis thaliana OX=3702 GN=RD21C PE=1 S...	[more]
P25777	6.4e-105	48.36	Oryzain beta chain OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0670200 PE=1...	[more]

BLAST of Lag0036987 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1IL34	3.6e-159	63.81	low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1F9F2	3.9e-158	63.81	low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1FLR7	6.7e-158	64.46	low-temperature-induced cysteine proteinase-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1J4D3	8.8e-158	64.46	low-temperature-induced cysteine proteinase-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1C3C7	3.8e-153	63.18	low-temperature-induced cysteine proteinase-like OS=Momordica charantia OX=3673 ...	[more]

BLAST of Lag0036987 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G47128.1	5.9e-122	50.77	Granulin repeat cysteine protease family protein	[more]
AT5G43060.1	6.5e-121	50.98	Granulin repeat cysteine protease family protein	[more]
AT3G19390.1	1.8e-110	49.53	Granulin repeat cysteine protease family protein	[more]
AT1G09850.1	6.5e-97	47.80	xylem bark cysteine peptidase 3	[more]
AT4G36880.1	8.5e-97	52.66	cysteine proteinase1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000668	Peptidase C1A, papain C-terminal	PRINTS	PR00705	PAPAIN	coord: 281..291 score: 58.16 coord: 140..155 score: 64.74 coord: 296..302 score: 73.06
IPR000668	Peptidase C1A, papain C-terminal	SMART	SM00645	pept_c1	coord: 122..337 e-value: 4.1E-106 score: 368.5
IPR000668	Peptidase C1A, papain C-terminal	PFAM	PF00112	Peptidase_C1	coord: 122..337 e-value: 4.9E-75 score: 252.4
IPR000118	Granulin	SMART	SM00277	GRAN_2	coord: 347..389 e-value: 2.1E-4 score: 27.0
IPR013201	Cathepsin propeptide inhibitor domain (I29)	SMART	SM00848	Inhibitor_I29_2	coord: 39..92 e-value: 9.8E-18 score: 74.9
IPR013201	Cathepsin propeptide inhibitor domain (I29)	PFAM	PF08246	Inhibitor_I29	coord: 39..92 e-value: 3.6E-11 score: 43.3
None	No IPR available	GENE3D	3.90.70.10	Cysteine proteinases	coord: 20..339 e-value: 3.8E-111 score: 373.9
None	No IPR available	PANTHER	PTHR12411:SF749	CYSTEINE PROTEASE	coord: 23..363
None	No IPR available	PANTHER	PTHR12411	CYSTEINE PROTEASE FAMILY C1-RELATED	coord: 23..363
None	No IPR available	SUPERFAMILY	57277	Granulin repeat	coord: 344..377
IPR025661	Cysteine peptidase, asparagine active site	PROSITE	PS00640	THIOL_PROTEASE_ASN	coord: 296..315
IPR000169	Cysteine peptidase, cysteine active site	PROSITE	PS00139	THIOL_PROTEASE_CYS	coord: 140..151
IPR025660	Cysteine peptidase, histidine active site	PROSITE	PS00639	THIOL_PROTEASE_HIS	coord: 279..289
IPR039417	Papain-like cysteine endopeptidase	CDD	cd02248	Peptidase_C1A	coord: 123..336 e-value: 1.63915E-99 score: 294.146
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 31..337

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0036987.1	Lag0036987.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
biological_process	GO:0006508	proteolysis
cellular_component	GO:0005615	extracellular space
cellular_component	GO:0005764	lysosome
molecular_function	GO:0004197	cysteine-type endopeptidase activity
molecular_function	GO:0008234	cysteine-type peptidase activity