Lag0036873 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0036873 vs. NCBI nr
Match: XP_022974919.1 (probable calcium-binding protein CML41 [Cucurbita maxima])

HSP 1 Score: 291.2 bits (744), Expect = 6.3e-75
Identity = 151/185 (81.62%), Postives = 159/185 (85.95%), Query Frame = 0

Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPP--LPLTAAPPFAEAAFRHLDA 60
           MA+S IS P KWFS TNFKLNFPRL  KHKPT S+ PPP  L LT APP AEAAFRHLDA
Sbjct: 15  MALSPISTPFKWFSNTNFKLNFPRLRFKHKPTPSNPPPPPLLLLTPAPPLAEAAFRHLDA 74

Query: 61  DGDGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYFAS+GEYMT DDAR +I ++DGDGD LLEL DFERL+K   EEEEK
Sbjct: 75  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG-GEEEEK 134

Query: 121 EDLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLKMAFEMFE EKGCGFIG  GLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 135 EDLKMAFEMFEGEKGCGFIGPPGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 194

Query: 181 LRMMT 184
           LRMMT
Sbjct: 195 LRMMT 198

BLAST of Lag0036873 vs. NCBI nr
Match: KAG6591338.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 286.2 bits (731), Expect = 2.0e-73
Identity = 149/185 (80.54%), Postives = 157/185 (84.86%), Query Frame = 0

Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPP--LPLTAAPPFAEAAFRHLDA 60
           MA+S IS P KWFS TN KLN PRL  KHKPT S+ PPP  L LT APP AEAAFRHLDA
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYFAS+GEYMT DDAR +I ++DGDGD LLEL DFERL+K   EEEEK
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDGDGDLLLELGDFERLVKG-GEEEEK 120

Query: 121 EDLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLK AFEMFE EKGCGFIG AGLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 121 EDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 180

Query: 181 LRMMT 184
           LRMMT
Sbjct: 181 LRMMT 184

BLAST of Lag0036873 vs. NCBI nr
Match: XP_023535419.1 (calmodulin-like protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 285.4 bits (729), Expect = 3.4e-73
Identity = 148/185 (80.00%), Postives = 157/185 (84.86%), Query Frame = 0

Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPP--LPLTAAPPFAEAAFRHLDA 60
           MA+S +S P KWFS TNF LNFPRL  KHKPT S+ PPP  L LT APP AEAAFRHLDA
Sbjct: 38  MALSPVSTPFKWFSNTNFNLNFPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 97

Query: 61  DGDGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYFAS+GEYMT DDAR +I ++D DGD LLEL DFERL+K   EEEEK
Sbjct: 98  DGDGKISGDELQSYFASVGEYMTRDDARSLIREFDWDGDLLLELGDFERLVKG-GEEEEK 157

Query: 121 EDLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLK AFEMFE EKGCGFIG AGLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 158 EDLKRAFEMFEGEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 217

Query: 181 LRMMT 184
           LRMMT
Sbjct: 218 LRMMT 221

BLAST of Lag0036873 vs. NCBI nr
Match: KAG7024217.1 (putative calcium-binding protein CML41, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 284.6 bits (727), Expect = 5.9e-73
Identity = 148/185 (80.00%), Postives = 157/185 (84.86%), Query Frame = 0

Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPP--LPLTAAPPFAEAAFRHLDA 60
           MA+S IS P KWFS TN KLN PRL  KHKPT S+ PPP  L LT APP AEAAFRHLDA
Sbjct: 1   MALSPISTPFKWFSNTNLKLNLPRLRFKHKPTRSNPPPPPLLLLTPAPPLAEAAFRHLDA 60

Query: 61  DGDGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLIKAEEEEEEK 120
           DGDGKIS DEL+SYFAS+GEYMT D+AR +I ++DGDGD LLEL DFERL+K   EEEEK
Sbjct: 61  DGDGKISGDELQSYFASVGEYMTRDNARSLIREFDGDGDLLLELGDFERLVKG-GEEEEK 120

Query: 121 EDLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREF 180
           EDLK AFEMFE EKGCGFIG AGLQKMFGRLGYVKS EECMAMIGVFDVDGDGVIDYREF
Sbjct: 121 EDLKRAFEMFEEEKGCGFIGPAGLQKMFGRLGYVKSIEECMAMIGVFDVDGDGVIDYREF 180

Query: 181 LRMMT 184
           LRMMT
Sbjct: 181 LRMMT 184

BLAST of Lag0036873 vs. NCBI nr
Match: XP_008466624.1 (PREDICTED: probable calcium-binding protein CML41 [Cucumis melo] >KAA0055432.1 putative calcium-binding protein CML41 [Cucumis melo var. makuwa])

HSP 1 Score: 283.9 bits (725), Expect = 1.0e-72
Identity = 146/183 (79.78%), Postives = 154/183 (84.15%), Query Frame = 0

Query: 1   MAISTISIPLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPLPLTAAPPFAEAAFRHLDADG 60
           MAISTI+IP KWF+KTNFKLNFP LHSKHKP +S  P P      PP AE AFRHLD DG
Sbjct: 1   MAISTIAIPFKWFTKTNFKLNFPLLHSKHKPISSKPPTP------PPLAEVAFRHLDIDG 60

Query: 61  DGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLIKAEEEEEEKED 120
           DGKIS DELRSYFASIGEYMT D A+ VIGD+D DGDELLEL DFERL+K EEEE+E ED
Sbjct: 61  DGKISGDELRSYFASIGEYMTWDGAQSVIGDFDKDGDELLELGDFERLVKGEEEEQE-ED 120

Query: 121 LKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGVIDYREFLR 180
           LK AFEMFE EKGCGFIGA GLQKMFGRLGYVKSKE+CM MI VFDVDGDGVIDY EFLR
Sbjct: 121 LKRAFEMFEGEKGCGFIGATGLQKMFGRLGYVKSKEDCMTMIKVFDVDGDGVIDYHEFLR 176

Query: 181 MMT 184
           MMT
Sbjct: 181 MMT 176

BLAST of Lag0036873 vs. ExPASy Swiss-Prot
Match: Q8L3R2 (Probable calcium-binding protein CML41 OS=Arabidopsis thaliana OX=3702 GN=CML41 PE=2 SV=2)

HSP 1 Score: 121.3 bits (303), Expect = 1.1e-26
Identity = 77/191 (40.31%), Postives = 104/191 (54.45%), Query Frame = 0

Query: 11  KWFSKTNFKLNFPRLHSKHKP-TTSSKPPPLPLT------------AAPPFAEAAFRHLD 70
           KWFS    KLN    + +  P + SS     P +            A+       F H D
Sbjct: 14  KWFSTKTLKLNLSFQNRRRSPKSNSSSTLNSPRSNSDDNNNIKSHQASKEELRQVFSHFD 73

Query: 71  ADGDGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDF-----ERLIKAE 130
           +DGDGKIS+ ELR YF S+GEY++ + A+  I + D D D  L   DF      R +  +
Sbjct: 74  SDGDGKISAFELRHYFGSVGEYISHEAAQEAINEVDTDADGSLGFEDFVGLMTRRDLYGD 133

Query: 131 EEEEEKEDLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFDVDGDGV 184
            E +   +LK AFEMFEVEKG G I   GLQKM  +LG  ++  EC AMI  +D+DG+G+
Sbjct: 134 GEVDGDGELKTAFEMFEVEKGSGCITPKGLQKMLVKLGESRTYGECEAMIKFYDIDGNGI 193

BLAST of Lag0036873 vs. ExPASy Swiss-Prot
Match: Q9SRE6 (Calcium-binding protein CML38 OS=Arabidopsis thaliana OX=3702 GN=CML38 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 1.0e-19
Identity = 50/133 (37.59%), Postives = 85/133 (63.91%), Query Frame = 0

Query: 50  EAAFRHLDADGDGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLI 109
           EA F ++DA+ DG+IS +EL+  F ++GE ++ ++A   +   D DGD +L+  +F +LI
Sbjct: 45  EAVFSYMDANRDGRISPEELQKSFMTLGEQLSDEEAVAAVRLSDTDGDGMLDFEEFSQLI 104

Query: 110 KAEEEEEEKEDLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFDVDG 169
           K ++EEE+K +LK AF ++ + +G   I    L+ M  +LG  ++ ++C  MI  FD++ 
Sbjct: 105 KVDDEEEKKMELKGAFRLY-IAEGEDCITPRSLKMMLKKLGESRTTDDCRVMISAFDLNA 164

Query: 170 DGVIDYREFLRMM 183
           DGV+ + EF  MM
Sbjct: 165 DGVLSFDEFALMM 176

BLAST of Lag0036873 vs. ExPASy Swiss-Prot
Match: Q8RYK0 (Probable calcium-binding protein CML31 OS=Oryza sativa subsp. japonica OX=39947 GN=CML31 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-18
Identity = 61/146 (41.78%), Postives = 88/146 (60.27%), Query Frame = 0

Query: 44  AAPPFAEAAFRHLDADGDGKISSDELRSYF-ASIGEYMTGDDARGVIGDYDGDGDELLEL 103
           A+P  + A F   D DGDG+IS+ ELR     ++GE ++ ++A  ++   D DGD LL  
Sbjct: 7   ASPCESSALFATFDHDGDGRISAAELRLCMKTTLGEEVSDEEAGQLVASVDADGDGLLCE 66

Query: 104 RDFERLIKAEEEEEEKE----DLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEEC 163
            +F RL++A E EEE E     L+ AF M+E+E G G I    L++M  RLG  +  ++C
Sbjct: 67  AEFVRLVQAAEVEEEDERRGTGLREAFGMYEME-GEGCITPTSLRRMLRRLGSDQDIDDC 126

Query: 164 MAMIGVFDVDGDGVIDYREFLRMMTA 185
            AMI  FD++GDGV+ + EF  MM A
Sbjct: 127 RAMICRFDLNGDGVLSFDEFKIMMNA 151

BLAST of Lag0036873 vs. ExPASy Swiss-Prot
Match: Q9SRE7 (Calcium-binding protein CML39 OS=Arabidopsis thaliana OX=3702 GN=CML39 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 2.1e-17
Identity = 50/136 (36.76%), Postives = 82/136 (60.29%), Query Frame = 0

Query: 50  EAAFRHLDADGDGKISSDELRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLI 109
           EA F ++DA+ DG+IS++EL+  F ++GE M+ ++A   +   D DGD +L++ +F  LI
Sbjct: 24  EAVFAYMDANRDGRISAEELKKSFKTLGEQMSDEEAEAAVKLSDIDGDGMLDINEFALLI 83

Query: 110 KAEE---EEEEKEDLKMAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSKEECMAMIGVFD 169
           K  +   EEE+K  +  AF M+ +  G   I    L+ M  +LG  ++ ++C  MI  FD
Sbjct: 84  KGNDEFTEEEKKRKIMEAFRMY-IADGEDCITPGSLKMMLMKLGESRTTDDCKVMIQAFD 143

Query: 170 VDGDGVIDYREFLRMM 183
           ++ DGV+ + EF  MM
Sbjct: 144 LNADGVLSFDEFALMM 158

BLAST of Lag0036873 vs. ExPASy Swiss-Prot
Match: O22845 (Calmodulin-like protein 5 OS=Arabidopsis thaliana OX=3702 GN=CML5 PE=2 SV=2)

HSP 1 Score: 88.6 bits (218), Expect = 8.1e-17
Identity = 63/182 (34.62%), Postives = 94/182 (51.65%), Query Frame = 0

Query: 9   PLKWFSKTNFKLNFPRLHSKHKPTTSSKPPPLPLTAAPPFAEAAFRHLDADGDGKISSDE 68
           PL WF KT  K N P   S   P+ SS   P      P   +  F+  D +GDG+I+ +E
Sbjct: 28  PLSWFDKTLHK-NSPPSPSTMLPSPSSSSAPTK-RIDPSELKRVFQMFDKNGDGRITKEE 87

Query: 69  LRSYFASIGEYMTGDDARGVIGDYDGDGDELLELRDFERLIKA------EEEEEEKEDLK 128
           L     ++G Y+   D   +I   D +GD  +++ +FE L  +       + E E+ED+K
Sbjct: 88  LNDSLENLGIYIPDKDLTQMIHKIDANGDGCVDIDEFESLYSSIVDEHHNDGETEEEDMK 147

Query: 129 MAFEMFEVEKGCGFIGAAGLQKMFGRLGYVKSK--EECMAMIGVFDVDGDGVIDYREFLR 183
            AF +F+ + G GFI    L+ +   LG  + K  + C  MI   D DGDG ++Y+EFL+
Sbjct: 148 DAFNVFD-QDGDGFITVEELKSVMASLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQ 206