Lag0036244 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0036244
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRibonuclease H-like domain containing protein
Locationchr3: 42366769 .. 42367338 (+)
RNA-Seq ExpressionLag0036244
SyntenyLag0036244
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAAGTTTGTGGGGAGAGTGTTGGAATTGTCGAAGTTTGACGGCCTTTTGCAGGTTCCTATGTCTGTGAGTATCTTGTTCTTCCTGATGGATATGAAGGAGAGAATGGATGGGGAATCTTTTGAGGAGTTTTCTATGATTCTGTGGGCTGTTTGGGGTTGCCAGAATAGGTGTAGAAGTTTGGGTGTTGCAACGACGATTGGTTTGTCAGATTGGGCAGTGGCGTATGTGGCCGTTTTTCGGGATGCAAGATGGGCAGGGGCTGGAAGTTTTGAGAGGCGGCTGAATCGGTCTGAGGTTAGGTGGGTTCAGTCGGCGTGGAATCAGTATAAGGTGAATGTGGATGCTTCCTCCTCACCGGAGTCGTCGTTGGCTAGTGTGGGGGTTGTCGTACGAGATTTGAAGAGGTTTGTTTTGCTCTATGCGATTAAATTTGTGGGCAGAATTCGGTGCTCTGATTTGGCAGAGGGACTGGCTGTTGCTGAGGGGATGAAGCTGGCGATGGAAATGGGTTTTAAGCTTGTGGTTGTTGAGACAGATTCGAGTAGAATTTTCCAGCTAGTGTAG

mRNA sequence

ATGTCTAAGTTTGTGGGGAGAGTGTTGGAATTGTCGAAGTTTGACGGCCTTTTGCAGGTTCCTATGTCTGTGAGTATCTTGTTCTTCCTGATGGATATGAAGGAGAGAATGGATGGGGAATCTTTTGAGGAGTTTTCTATGATTCTGTGGGCTGTTTGGGGTTGCCAGAATAGGTGTAGAAGTTTGGGTGTTGCAACGACGATTGGTTTGTCAGATTGGGCAGTGGCGTATGTGGCCGTTTTTCGGGATGCAAGATGGGCAGGGGCTGGAAGTTTTGAGAGGCGGCTGAATCGGTCTGAGGTTAGGTGGGTTCAGTCGGCGTGGAATCAGTATAAGGTGAATGTGGATGCTTCCTCCTCACCGGAGTCGTCGTTGGCTAGTGTGGGGGTTGTCGTACGAGATTTGAAGAGGTTTGTTTTGCTCTATGCGATTAAATTTGTGGGCAGAATTCGGTGCTCTGATTTGGCAGAGGGACTGGCTGTTGCTGAGGGGATGAAGCTGGCGATGGAAATGGGTTTTAAGCTTGTGGTTGTTGAGACAGATTCGAGTAGAATTTTCCAGCTAGTGTAG

Coding sequence (CDS)

ATGTCTAAGTTTGTGGGGAGAGTGTTGGAATTGTCGAAGTTTGACGGCCTTTTGCAGGTTCCTATGTCTGTGAGTATCTTGTTCTTCCTGATGGATATGAAGGAGAGAATGGATGGGGAATCTTTTGAGGAGTTTTCTATGATTCTGTGGGCTGTTTGGGGTTGCCAGAATAGGTGTAGAAGTTTGGGTGTTGCAACGACGATTGGTTTGTCAGATTGGGCAGTGGCGTATGTGGCCGTTTTTCGGGATGCAAGATGGGCAGGGGCTGGAAGTTTTGAGAGGCGGCTGAATCGGTCTGAGGTTAGGTGGGTTCAGTCGGCGTGGAATCAGTATAAGGTGAATGTGGATGCTTCCTCCTCACCGGAGTCGTCGTTGGCTAGTGTGGGGGTTGTCGTACGAGATTTGAAGAGGTTTGTTTTGCTCTATGCGATTAAATTTGTGGGCAGAATTCGGTGCTCTGATTTGGCAGAGGGACTGGCTGTTGCTGAGGGGATGAAGCTGGCGATGGAAATGGGTTTTAAGCTTGTGGTTGTTGAGACAGATTCGAGTAGAATTTTCCAGCTAGTGTAG

Protein sequence

MSKFVGRVLELSKFDGLLQVPMSVSILFFLMDMKERMDGESFEEFSMILWAVWGCQNRCRSLGVATTIGLSDWAVAYVAVFRDARWAGAGSFERRLNRSEVRWVQSAWNQYKVNVDASSSPESSLASVGVVVRDLKRFVLLYAIKFVGRIRCSDLAEGLAVAEGMKLAMEMGFKLVVVETDSSRIFQLV
Homology
BLAST of Lag0036244 vs. NCBI nr
Match: XP_022150944.1 (uncharacterized protein LOC111018973 [Momordica charantia])

HSP 1 Score: 84.7 bits (208), Expect = 9.2e-13
Identity = 61/183 (33.33%), Postives = 91/183 (49.73%), Query Frame = 0

Query: 10  ELSKFDGLLQVPMSVSILFFLMDMKERMDGESFEEFSMILWAVWGCQNRCR---SLGVAT 69
           + S F  LL VP+   +  F+    + +  +      ++LWA+W  +N+     SLG  +
Sbjct: 9   KFSHFLHLLWVPVGSDMGHFMRVWTDLVSWQHIGAIVVLLWAIWNARNQTPQHFSLG-GS 68

Query: 70  TIGLSDWAVAYVAVFRDARWAGAGSFERRLNRSEVRWVQSAWNQYKVNVDASSSPESSLA 129
              L  W+  Y+ V++ A+ +   +           W   A    KVNVDA+   ES +A
Sbjct: 69  LSDLVSWSENYLQVYQAAQRSSXSALVVCRPPRVAIWRPPAAPLLKVNVDAAFRKESFVA 128

Query: 130 SVGVVVRDLKRFVLLYAIKFVGRIRCSDLAEGLAVAEGMKLAMEMGFKLVVVETDSSRIF 189
            VGV++RD    V L AI+ + R    D  EG AV EG+ LA+E GF    +ETDS RIF
Sbjct: 129 GVGVIIRDSTGLVYLTAIRLLARASDVDWVEGFAVYEGILLAVEAGFIRFQIETDSLRIF 188

BLAST of Lag0036244 vs. NCBI nr
Match: XP_022140628.1 (uncharacterized protein LOC111011237 [Momordica charantia])

HSP 1 Score: 77.4 bits (189), Expect = 1.5e-10
Identity = 45/120 (37.50%), Postives = 70/120 (58.33%), Query Frame = 0

Query: 70  LSDWAVAYVAVFRDARWAGAGSFERRL-NRSEVRWVQSAWNQYKVNVDASSSPESSLASV 129
           L +WA  YV  FR+   A +  F  R+ N +EV W+      YK+N DAS       A +
Sbjct: 3   LVEWANKYVMEFRE---ANSNPFPGRVTNTAEVLWLPPDKRIYKINTDASFLASDQHAGL 62

Query: 130 GVVVRDLKRFVLLYAIKFVGRIRCSDLAEGLAVAEGMKLAMEMGFKLVVVETDSSRIFQL 189
           G+++R+ +  V+  A K++  I+  D+AE +   EG++LA ++G   V++ETDSSRIF L
Sbjct: 63  GIIIRNDRGQVMASATKYLENIQSVDMAEAIVAVEGLQLASKIGVNPVILETDSSRIFNL 119

BLAST of Lag0036244 vs. NCBI nr
Match: XP_022139684.1 (uncharacterized protein LOC111010533 [Momordica charantia])

HSP 1 Score: 70.9 bits (172), Expect = 1.4e-08
Identity = 49/172 (28.49%), Postives = 83/172 (48.26%), Query Frame = 0

Query: 30  LMDMKERMDGESFEEFSMILWAVWGCQNR-CRSLGVATTIGLSDWAVAYVAVFR------ 89
           L D ++ ++ + FEE  + LW++W  +N    +        L+ W   Y+A F+      
Sbjct: 5   LRDWRDMLNWKDFEELVVFLWSLWNRRNAFVFNKRRVEDDDLAGWVSTYIATFKATNTNH 64

Query: 90  -----DARWAGAGSFERRLNRSEVRWVQSAWNQYKVNVDASSSPESSLASVGV-VVRDLK 149
                D   +   S +    ++   W  +    +K+  DAS S     A +GV ++RD +
Sbjct: 65  ATANQDVSQSFQQSSQIHQAQNHTIWCPAEEGVFKLKTDASFSSIDFNAGLGVIIIRDHR 124

Query: 150 RFVLLYAIKFVGRIRCSDLAEGLAVAEGMKLAMEMGFKLVVVETDSSRIFQL 189
             VL  A K++  +   D AE LA  EG+++AME G   +++ETDS RI+ L
Sbjct: 125 GQVLASATKYLEHVASVDDAEALAAVEGLRVAMETGISPILLETDSLRIYNL 176

BLAST of Lag0036244 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-08
Identity = 44/155 (28.39%), Postives = 75/155 (48.39%), Query Frame = 0

Query: 24   VSILFFLMDMKERMDGESFEEFSMILWAVWGCQNRCRSLGVATT------IGLSDWAVAY 83
            +S    L +  E +    FEE  +++W +W  Q   R+   +T       + L +WA  Y
Sbjct: 909  LSPFLILRESHESLSKADFEELCVVIWGLWN-QRNARAFNDSTKTVFKIGMELVEWANKY 968

Query: 84   VAVFRDARWAGAGSFERRLNRSEVRWVQSAWNQYKVNVDASSSPESSLASVGVVVRDLKR 143
               FR+A+        R  N +E+ W       YK+N DAS       A +G+++ + + 
Sbjct: 969  AMEFREAK--SNPITGRVTNTAEILWQPPDEGIYKINTDASFLASDQHAGLGIIIHNDRG 1028

Query: 144  FVLLYAIKFVGRIRCSDLAEGLAVAEGMKLAMEMG 173
             V+  A K++  I+  D+AE +A  EG++LA E+G
Sbjct: 1029 QVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIG 1060

BLAST of Lag0036244 vs. NCBI nr
Match: XP_022135942.1 (uncharacterized protein LOC111007775 [Momordica charantia])

HSP 1 Score: 70.1 bits (170), Expect = 2.3e-08
Identity = 49/159 (30.82%), Postives = 79/159 (49.69%), Query Frame = 0

Query: 35  ERMDGESFEEFSMILWAVWGCQNRCRSLG--VATTIGLSDWAVAYVAVFRDARWAGAGS- 94
           +R+     EE S+ LWA+W  +N+           + + DW   Y+ V++ A+   +   
Sbjct: 2   DRIKWTGLEELSVFLWAIWNARNQSMFTNGHGFLLVNMVDWVADYLQVYQSAQEGPSLPL 61

Query: 95  -FERRLNRSEVRWVQSAWNQYKVNVDASSSPESSLASVGVVVRDLKRFVLLYAIKFVGRI 154
             + R  R    W       +KVNVDA+    +  A + +++RD    VLL A+ F+  +
Sbjct: 62  LLDGRGGRVSA-WCPPLAPFFKVNVDAAFKKGNFSAGLDILIRDSTVNVLLSAMCFITHV 121

Query: 155 RCSDLAEGLAVAEGMKLAMEMGFKLVVVETDSSRIFQLV 190
               LAE LA  EG+ LA+E G     +ETDSS++F L+
Sbjct: 122 SDVTLAECLAAQEGVCLAIEAGLIPFQIETDSSQVFNLL 159

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150944.19.2e-1333.33uncharacterized protein LOC111018973 [Momordica charantia][more]
XP_022140628.11.5e-1037.50uncharacterized protein LOC111011237 [Momordica charantia][more]
XP_022139684.11.4e-0828.49uncharacterized protein LOC111010533 [Momordica charantia][more]
XP_022150918.12.3e-0828.39uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_022135942.12.3e-0830.82uncharacterized protein LOC111007775 [Momordica charantia][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 114..189
e-value: 5.9E-11
score: 42.3
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 108..189
e-value: 1.9E-7
score: 33.1
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 109..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0036244.1Lag0036244.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity