Lag0034451 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0034451 vs. NCBI nr
Match: XP_022933804.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 2132.8 bits (5525), Expect = 0.0e+00
Identity = 1127/1342 (83.98%), Postives = 1210/1342 (90.16%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATGT TAS+ILHH SAFI+EILAQS+LRKRLLS VYG +SLSDEIALKPL LAAEALE
Sbjct: 1    MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLSLPEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF+EQRS+ILT  PSNL  N+TE+SI DES VF C
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKW EME+I E+K RTEQL LPNGRYNPIW+EEE+   +L+VD T KSK      PP V
Sbjct: 241  NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQ---TLQVDCTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEGDVQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLLSVVKKKEEDVNKMRLS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVE RIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS DEQFEIIMFLSELVKLNRRT NE+IL+NIKDG  
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSK 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMD LISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRF+TSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIE KAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSVRGTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQ H+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQAHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKIDTLQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1323

BLAST of Lag0034451 vs. NCBI nr
Match: XP_023007456.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 2127.8 bits (5512), Expect = 0.0e+00
Identity = 1123/1342 (83.68%), Postives = 1210/1342 (90.16%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATG  TAS+ILHH SAFI+EILAQS+LRKRLLS VYG ISLSDEIALKPL LAAEALE
Sbjct: 1    MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLS+PEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF++QR +ILT  PSNL  N+TE+SI DE  VFPC
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKWKEME+I ++K RTEQL  PNGRYNPIW+EEE+   +LEVD T KSK      PP V
Sbjct: 241  NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQ---TLEVDFTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEG+VQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGNVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLL+VVKKKEEDVNKM LS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVEERIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS  EQFEIIMFLSELVKLNRRT NE+IL+NIKDG +
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNGEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSE 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMDVLISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRFSTSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIEEKAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSV+GTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVKGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQVH+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQVHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKID LQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1323

BLAST of Lag0034451 vs. NCBI nr
Match: XP_022933805.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 2126.3 bits (5508), Expect = 0.0e+00
Identity = 1126/1342 (83.90%), Postives = 1209/1342 (90.09%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATGT TAS+ILHH SAFI+EILAQS+LRKRLLS VYG +SLSDEIALKPL LAAEALE
Sbjct: 1    MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLSLPEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF+EQRS+ILT  PSNL  N+TE+SI DES VF C
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKW EME+I E+K RTEQL LPNGRYNPIW+EEE+   +L+VD T KSK      PP V
Sbjct: 241  NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQ---TLQVDCTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEGDVQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLLSVVKKKEEDVNKMRLS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVE RIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS DEQFEIIMFLSELVKLN RT NE+IL+NIKDG  
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSK 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMD LISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRF+TSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIE KAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSVRGTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQ H+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQAHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKIDTLQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1322

BLAST of Lag0034451 vs. NCBI nr
Match: XP_023007466.1 (putative E3 ubiquitin-protein ligase LIN-1 isoform X2 [Cucurbita maxima])

HSP 1 Score: 2121.3 bits (5495), Expect = 0.0e+00
Identity = 1122/1342 (83.61%), Postives = 1209/1342 (90.09%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATG  TAS+ILHH SAFI+EILAQS+LRKRLLS VYG ISLSDEIALKPL LAAEALE
Sbjct: 1    MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLS+PEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF++QR +ILT  PSNL  N+TE+SI DE  VFPC
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKWKEME+I ++K RTEQL  PNGRYNPIW+EEE+   +LEVD T KSK      PP V
Sbjct: 241  NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQ---TLEVDFTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEG+VQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGNVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLL+VVKKKEEDVNKM LS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVEERIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS  EQFEIIMFLSELVKLN RT NE+IL+NIKDG +
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNGEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSE 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMDVLISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRFSTSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIEEKAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSV+GTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVKGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQVH+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQVHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKID LQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1322

BLAST of Lag0034451 vs. NCBI nr
Match: KAG7021867.1 (putative E3 ubiquitin-protein ligase LIN-1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 2118.6 bits (5488), Expect = 0.0e+00
Identity = 1127/1362 (82.75%), Postives = 1210/1362 (88.84%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATGT TAS+ILHH SAFI+EILAQS+LRKRLLS VYG ISLSDEIALKPL LAAEALE
Sbjct: 1    MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLSLPEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF+EQRS+ILT  PSNL  N+TE SI DES VFPC
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTERSIGDESEVFPC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKWKEME+I E+K RTEQL LPNGRYNPIW+EEE+   +L+VD T KSK      PP V
Sbjct: 241  NEKWKEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQ---TLQVDCTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEGDV+HMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGDVRHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLL+VVKKKEEDVNKMRLS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLNVVKKKEEDVNKMRLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVEERIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS DEQFEIIMFLSELVKLNRRT NE+IL+NIKDG  
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSK 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMD LISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRF+TSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIE KAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTVTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSVRGTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIR------------------------VYSWDG 1255
            IQVH+VK+QIHNLV+SK+VACFIPHG GIR                        VYSW G
Sbjct: 1141 IQVHDVKEQIHNLVVSKSVACFIPHGVGIRVSHQILHYRIPFYTLYSEEDRHWLVYSWAG 1200

Query: 1256 ESKVLNSSKYVKCLNLVHGKLYCGCHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQAL 1315
            ESK+LNSSK+VKCLNL+HGKLYCGCHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ AL
Sbjct: 1201 ESKLLNSSKHVKCLNLMHGKLYCGCHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHAL 1260

Query: 1316 QVYDEQLFSASTALDGAAVKIWSTSNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEI 1375
            QVYDEQLFSASTALDGAAVKI   SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEI
Sbjct: 1261 QVYDEQLFSASTALDGAAVKIRCRSNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEI 1320

Query: 1376 WGREKHNKIDTLQIGRNCKVVCMTLDEREEVLVAGTSDGQIQ 1393
            W REKHNKIDTLQIGRNCKVVCM+L+ERE+VLV GTSDG+IQ
Sbjct: 1321 WSREKHNKIDTLQIGRNCKVVCMSLNEREDVLVIGTSDGRIQ 1343

BLAST of Lag0034451 vs. ExPASy Swiss-Prot
Match: D1FP53 (Putative E3 ubiquitin-protein ligase LIN OS=Medicago truncatula OX=3880 GN=LIN PE=2 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 7.3e-211
Identity = 500/1451 (34.46%), Postives = 768/1451 (52.93%), Query Frame = 0

Query: 95   KISLSDEIALKPLWLAAEALENAISADNSSIKSSSLRCAEELL------------LSLPE 154
            ++  SD+  L  L    EALE AI+  N   K + L  AE++L              +P 
Sbjct: 59   EVEYSDQAVLANLDWGIEALEEAINTYNMETKLARLDYAEKMLQVCAMLNPKQKTAGVPN 118

Query: 155  NPFSSFL---LSLIYGLNHQNLNSALSLLDLFLSDPSSARSEIAPILFEELFLRHFLPIF 214
            +  S++    LS ++ L +   +     L++F+ DP  +R + AP L++ LFL H   I 
Sbjct: 119  SYLSAWAHLNLSYLWKLRNNIKSCIYHSLEMFIVDPFFSRIDFAPELWKNLFLPHMSSIV 178

Query: 215  HWFNEQRSKILTS-LPSNLNQNSTE--YSIDDESVVFPCTKSLSKLSIDQTLKLKELESN 274
             W++E+R K++   LP + + + T     + +ES+VF        +  +Q  KL++LE  
Sbjct: 179  GWYSEERHKLMMEVLPESTDFSYTADFDKVFNESLVF-------SMRPNQLEKLQKLEQL 238

Query: 275  YERVLDKNCRVFAGHFKRVLERKDDGG-------TIANPKVRLLEK-------------- 334
            Y   LD+N R++A ++   +               IA P +  L +              
Sbjct: 239  YGESLDENTRLYAKYYNDCMNPDSTSSKKVVPMLPIAEPPMTPLHELSRSVPDFVKFGPI 298

Query: 335  -----------KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVD----- 394
                       +     + E T E   +   +   G  + +W  +E  I  +E D     
Sbjct: 299  LPKSSGFSMTTRRSNDGLNETTRENIASNS-NHSKGEQSSLWAAKESIIEEIEDDLDSEH 358

Query: 395  -----STTKSKLPRPPPPPSVHSQILGPFNFLSEQ------------ESSSRSITFSIFN 454
                  + K  +  P P  ++  + + P  + S Q            ES  R+  +S  N
Sbjct: 359  YDASVDSDKINIFSPEPKKNIKDEDVEPKVYRSNQKNQMNSPNISPMESPRRASNYSSTN 418

Query: 455  PAAEAQDD---------------SSESFSLGSETEIEENDHETTLFGSEEPKPETQKLEQ 514
            P    ++                S  S S   +T    +DH   +F  +E       +++
Sbjct: 419  PLRRKKESKFLRLLSNRFTGSIVSDHSLSSSPDT---SSDH---IFTGDEEVMVRNNIKR 478

Query: 515  TITSMEESITQSVELDFAMEDSGNASPESGGRHNT--------------PKDFVCPITCN 574
               S   S+ Q  E    + DS +   E G + ++              PKDFVCPIT  
Sbjct: 479  KNDSQTPSMNQDNENSLVLNDSSHCESEDGYQSSSSFPKLEKLTIGSKPPKDFVCPITGQ 538

Query: 575  IFYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEEN 634
            IF DPVT+ETGQTYER AIQEWL  GN+TCPIT Q L    LPKTNYVLKRLI SW E+N
Sbjct: 539  IFSDPVTLETGQTYERKAIQEWLGTGNTTCPITRQALSANILPKTNYVLKRLIVSWKEQN 598

Query: 635  PNFV----------------------------------SEKALDE--------ADPVAVL 694
            P                                     S+K  D+           V+V 
Sbjct: 599  PELAQEFSNSNTPRGSSCSPSAKDITMVSSIQRTTDSPSQKYKDDYIRQRNNRFTRVSVG 658

Query: 695  ASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVERFWLEENVEGDVQHMLL 754
            ASP SV SQA+++  +  +   IT+LC S  L++ E AVL + R W +   +  +   L 
Sbjct: 659  ASPTSVLSQAAVETIINSLTPYITSLCTSENLQDCEQAVLEIARLWKDSKTDPQIHSYLS 718

Query: 755  KPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLTRVESDVDCIVTLFKRGL 814
            KP+V++GLVEIL  S+NR+VLR +I++LSEL   D+ V +TL  V+SD DC+  L K GL
Sbjct: 719  KPTVVSGLVEILSASLNREVLRRSIYILSELIFSDERVGETLNSVDSDFDCLAMLLKNGL 778

Query: 815  VEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS--HKSAAVLLLRKILER 874
             EA +LIYQL      L E +++ SL+ V++ K ED++  +L+   K+AA+ +L +IL  
Sbjct: 779  AEAALLIYQLRPVFAQLSEHELIPSLIQVIQNKSEDIDDFQLAIDPKAAAIAILEQILIG 838

Query: 875  SKEGSPIV--VSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQDGKCRNIIAVKAD 934
              E +  V   SV++ N   +I+  L   + E R   + ILL C+Q +  C++ IA + +
Sbjct: 839  GDEYNRSVNASSVISANGIPAIVKYL--DKTEGRRPVISILLCCMQAEKSCKSSIANRIE 898

Query: 935  LAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGDCSTMHSLLIYLQT 994
            L+P+LE F   +   +   + FLSELV+LNRRT + Q LQ IKD G  STMH+ L+YLQ 
Sbjct: 899  LSPVLELFHAGNDSVRGICVEFLSELVRLNRRTSSNQTLQIIKDEGAFSTMHTFLVYLQM 958

Query: 995  AIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPTAQISAAETIMSLQ 1054
            A  +    VA LLLQLD L EP+KMSIYREEA++ LI  L   +F   Q+ A + ++ L 
Sbjct: 959  APMEHQIAVASLLLQLDLLAEPRKMSIYREEAVETLIEALWQKDFSNNQMKALDALLFLI 1018

Query: 1055 GRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLEQKDEDKAANEWER 1114
            G  ++SG+      LL+ AG  + +   ++ + L  +  +   T    +DE  A   W++
Sbjct: 1019 GHVTSSGKSYTEAGLLKIAGFDQPYNVLMKAEQLGHSDNDFMET---MEDEKNAMKSWQK 1078

Query: 1115 KMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTLPDTGILEAARVFL 1174
            ++A VL +H+ G + ++L + LKS    +  +C V ATWL+HML TLPDTG+ + AR  L
Sbjct: 1079 RVASVLCNHENGSIFQALEECLKSNSLKMAKSCLVLATWLTHMLFTLPDTGVRDVARKSL 1138

Query: 1175 LDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRGLRELKRSTPLAFD 1234
            L+  +++  ++ ++EEK L  LA+ SF+ +P   + L    K+I R LR+LK+ + +A D
Sbjct: 1139 LEALMNVLQSSKNLEEKILASLALKSFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAAD 1198

Query: 1235 MLKVLCE-GQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHSDGTIKVWSVRGT 1294
            +LK L        TELWS +E+ + D   NGEVLS+ +  G+++SGH+DGTIKVW  R  
Sbjct: 1199 ILKALLNLNSVDVTELWSCKEVVELDLSSNGEVLSLHYLNGQVLSGHADGTIKVWDARKR 1258

Query: 1295 NLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQCIQVHNVKDQIHN 1354
               V+QE +EH K VTSL    S +KLYS SLDKTI++W++  D I+CI V++VK+ ++ 
Sbjct: 1259 IPRVIQETREHKKAVTSLC--SSVDKLYSSSLDKTIRVWTIKPDGIKCIDVYDVKEAVYE 1318

Query: 1355 LVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCGCHDGSIQEVDLA 1397
            L  +  +AC++  G G++V++W    K +N +KYVKCL +   KLYCGC   SIQEVDL+
Sbjct: 1319 LAANAKLACYVTQGTGVKVFNWLDAPKFINFNKYVKCLAVSGDKLYCGCSGYSIQEVDLS 1378

BLAST of Lag0034451 vs. ExPASy Swiss-Prot
Match: C6L7U1 (Putative E3 ubiquitin-protein ligase LIN-1 OS=Lotus japonicus OX=34305 GN=CERBERUS PE=2 SV=2)

HSP 1 Score: 704.9 bits (1818), Expect = 1.8e-201
Identity = 432/1125 (38.40%), Postives = 643/1125 (57.16%), Query Frame = 0

Query: 329  EEEQIISLEVDSTTKSKLPRP---PPPPSVHSQILGPFNFLSEQESSSRSITFSIFNPAA 388
            +  QI S ++ S   S    P    P P +HS+    F  LS       +I+ S+     
Sbjct: 382  QRNQIPSPDIFSPLDSPRTAPNNSSPNPDMHSKRDSKFLRLSSSRIREPTISDSL----- 441

Query: 389  EAQDDSSESFSLGSETEIEENDHETTLFGSEEPKPETQKLEQTITSMEESITQSVELDFA 448
                 SS   S+ +   I   D+E  +  + + K + Q L     +    I     L   
Sbjct: 442  ----TSSPDISIDN---ISNADNEVMVLKNIQRKNDNQTLSMNHENENSLILNGSSL--C 501

Query: 449  MEDSGNASPESGGR-------HNTPKDFVCPITCNIFYDPVTVETGQTYERSAIQEWLDR 508
              D G  S  S  +          PKDFVCPIT  IF DPVT+ETGQTYER AIQEWL  
Sbjct: 502  ESDDGYQSFNSLPKLEKLSMGSKPPKDFVCPITGQIFCDPVTLETGQTYERKAIQEWLRT 561

Query: 509  GNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVSE------------------- 568
            GN+TCPIT Q L  + LPKTNYVLKRLI SW E+NP    E                   
Sbjct: 562  GNTTCPITRQPLSASILPKTNYVLKRLITSWKEQNPELAQEFSNVNTPRGSSCSPSAKDI 621

Query: 569  ---------------KALDEAD-------PVAVLASPVSVSSQASIDKSMKEVRLAITNL 628
                           K  D A        P A+  SP SV SQA+++  +  ++  I++L
Sbjct: 622  PMLSTRQRTTDSPNHKNKDYARQRSNRFMPAAITTSPTSVLSQAAVETIVNSLKPYISSL 681

Query: 629  CASGVLEEAETAVLCVERFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIF 688
            C S  L E E AVL + R   +      +   L KP++INGLVEIL  S NR+VLR +I+
Sbjct: 682  CTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASRNREVLRTSIY 741

Query: 689  LLSELGSKDDAVVQTLTRVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSL 748
            +LSEL   DD+V +TL  V+SD DC+ TL K GL EA +LIYQL      L   +++ SL
Sbjct: 742  ILSELIFTDDSVAETLNSVDSDFDCLATLLKNGLAEAALLIYQLRPVFAQLSAHELIPSL 801

Query: 749  LSVVKKKEEDVNKMRL--SHKSAAVLLLRKILERSKEGSPIV--VSVLAENATESILGSL 808
            + V++ K E+++  +L    K AA+ +L + L    E S  +   SV++ N   +++  L
Sbjct: 802  VDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVISANGIPTLVKYL 861

Query: 809  KAKQVEERIAAVGILLRCIQQDGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSEL 868
              +++E R + V +LL C+Q +  C+N+IA + +L+P+LE F   +   +   + FLSEL
Sbjct: 862  --ERMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELFHSGNDSVRGTCVEFLSEL 921

Query: 869  VKLNRRTFNEQILQNIKDGGDCSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMS 928
            V+LNRRT   QIL  IKD G  STMH+ L+YLQ A  +    VA LLLQLD L EP+KMS
Sbjct: 922  VQLNRRTSCNQILHTIKDEGAFSTMHTFLVYLQMAPMEHQLAVASLLLQLDLLAEPRKMS 981

Query: 929  IYREEAMDVLISCLRDSEFPTAQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHR 988
            IYREEA++ LI  L   +F   Q+ A + ++ L G  S+SG+     +LL+ AG  + + 
Sbjct: 982  IYREEAVETLIEALWQKDFSNTQMKALDALLFLIGHISSSGKSYTEAWLLKIAGFDQPYN 1041

Query: 989  KPIRRDNLSSALGEIELTRLEQKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKY 1048
              ++ + L     ++  T    +DE  A N W++++A VL +H+ G + ++L + LKS  
Sbjct: 1042 ALMKVEQLGQHDNDLIET---MEDEKNALNSWQKRIASVLCNHENGSIFKALEECLKSNS 1101

Query: 1049 AALFSACFVSATWLSHMLRTLPDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINS 1108
              +  +C V ATWL+HML TLPDTG+ + AR  LL+  +++  ++ ++EEK L  LA+ +
Sbjct: 1102 LKMAKSCLVLATWLTHMLYTLPDTGVRDVARKSLLEEVINVLQSSKNLEEKILATLALKT 1161

Query: 1109 FVHEPEGLQCLSSNMKNIMRGLRELKRSTPLAFDMLKVLCEGQD-SSTELWSHRELFQAD 1168
            F+ +P   + L    K+I R LR LK+ + +A D++KV+   +    TELWS +E+ + D
Sbjct: 1162 FISDPSTHEALRVYAKSIYRTLRRLKKYSVVAVDIMKVILNLKSVDVTELWSCKEVVELD 1221

Query: 1169 CRKNGEVLSIAHFKGKIISGHSDGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEK 1228
               NGEVLS+ +  G+++SGH+DGTIKVW  R     V+QE  EH+K VTSL    S ++
Sbjct: 1222 LSSNGEVLSMVYLNGQVLSGHTDGTIKVWDARKRIPRVIQETHEHTKAVTSLC--SSGDR 1281

Query: 1229 LYSGSLDKTIKIWSLGSDIIQCIQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGES 1288
            LYSGSLDKTI++W++ SD I+CI V+++K+ +H L  +  +AC++  G G++V++W    
Sbjct: 1282 LYSGSLDKTIRVWTIKSDGIKCIDVYDIKEAVHELAANDKLACYVSQGTGVKVFNWSEAP 1341

Query: 1289 KVLNSSKYVKCLNLVHGKLYCGCHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQV 1348
            K++N SKYVK L +   KLYCGC   SIQEVDL+T T +   +G+RKLLGK   + +LQ+
Sbjct: 1342 KLINFSKYVKSLAVAGDKLYCGCSGYSIQEVDLSTYTSNSFFTGTRKLLGK-QTIHSLQI 1401

Query: 1349 YDEQLFSASTALDGAAVKIWSTSNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWG 1397
            +D+ LF+  +++D  A KI+S S   V+GSL+T +D+  +A+  +  + G K G +E+W 
Sbjct: 1402 HDDYLFACGSSVDATAGKIFSLSQKMVVGSLSTGLDIHRIAINSDFIFAGTKFGTIEVWL 1461

BLAST of Lag0034451 vs. ExPASy Swiss-Prot
Match: D1FP57 (Putative E3 ubiquitin-protein ligase LIN-2 OS=Lotus japonicus OX=34305 GN=LIN PE=1 SV=1)

HSP 1 Score: 680.6 bits (1755), Expect = 3.7e-194
Identity = 425/1132 (37.54%), Postives = 637/1132 (56.27%), Query Frame = 0

Query: 329  EEEQIISLEVDSTTKSKLPRP---PPPPSVHSQILGPFNFLSEQESSSRSITFSIFNPAA 388
            +  QI S ++ S   S    P    P P +HS+    F  LS       +I+ S+     
Sbjct: 382  QRNQIPSPDIFSPLDSPRTAPNNSSPNPDMHSKRDSKFLRLSSSRIREPTISDSL----- 441

Query: 389  EAQDDSSESFSLGSETEIEENDHETTLFGSEEPKPETQKLEQTITSMEESITQSVELDFA 448
                 SS   S+ +   I   D+E  +  + + K ++Q           S+ Q  E    
Sbjct: 442  ----TSSPDISIDN---ISNADNEVMVRNNIKRKNDSQ---------TPSMNQDNENSLV 501

Query: 449  MEDSGNASPESGGRHNT--------------PKDFVCPITCNIFYDPVTVETGQTYERSA 508
            + DS +   E G + ++              PKDFVCPIT  IF DPVT+ETGQTYER A
Sbjct: 502  LNDSSHCESEDGYQSSSSLPKLEKLSMGSKPPKDFVCPITGQIFCDPVTLETGQTYERKA 561

Query: 509  IQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENPNFVSE------------ 568
            IQEWL  GN+TCPIT Q L  + LPKTNYVLKRLI SW E+NP    E            
Sbjct: 562  IQEWLRTGNTTCPITRQPLSASILPKTNYVLKRLITSWKEQNPELAQEFSNVNTPRGSSC 621

Query: 569  ----------------------KALDEAD-------PVAVLASPVSVSSQASIDKSMKEV 628
                                  K  D A        P A+  SP SV SQA+++  +  +
Sbjct: 622  SPSAKDIPMLSTRQRTTDSPNHKNKDYARQRSNRFMPAAITTSPTSVLSQAAVETIVNSL 681

Query: 629  RLAITNLCASGVLEEAETAVLCVERFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQ 688
            +  I++LC S  L E E AVL + R   +      +   L KP++INGLVEIL  S NR+
Sbjct: 682  KPYISSLCTSENLPECEEAVLKIARLLKDSKTNPQIHSYLSKPTIINGLVEILSASRNRE 741

Query: 689  VLRAAIFLLSELGSKDDAVVQTLTRVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKE 748
            VLR +I++LSEL   DD+V +TL  V+SD DC+ TL K GL EA +LIYQL      L  
Sbjct: 742  VLRTSIYILSELIFTDDSVAETLNSVDSDFDCLATLLKNGLAEAALLIYQLRPVFAQLSA 801

Query: 749  MDMVGSLLSVVKKKEEDVNKMRL--SHKSAAVLLLRKILERSKEGSPIV--VSVLAENAT 808
             +++ SL+ V++ K E+++  +L    K AA+ +L + L    E S  +   SV++ N  
Sbjct: 802  HELIPSLVDVIQNKNEELDDFQLVIDPKDAAIAILEQTLMGGDEYSRSLNASSVISANGI 861

Query: 809  ESILGSLKAKQVEERIAAVGILLRCIQQDGKCRNIIAVKADLAPILESFAEVSKDEQFEI 868
             +++  L  +++E R + V +LL C+Q +  C+N+IA + +L+P+LE F   +   +   
Sbjct: 862  PTLVKYL--ERMEGRRSVVSVLLCCMQAEKSCKNLIANRIELSPVLELFHSGNDSVRGTC 921

Query: 869  IMFLSELVKLNRRTFNEQILQNIKDGGDCSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNL 928
            + FLSELV+LNRRT   Q+L  IKD G  STMH+ L+YLQ A  +    VA LLLQLD L
Sbjct: 922  VEFLSELVQLNRRTSCNQLLHTIKDEGAFSTMHTFLVYLQMAPMEHQLAVASLLLQLDLL 981

Query: 929  VEPQKMSIYREEAMDVLISCLRDSEFPTAQISAAETIMSLQGRFSTSGRPLARYFLLERA 988
             EP+KMSIYREEA++ LI  L   +F   Q+ A + ++ L G  S+SG+     +LL+ A
Sbjct: 982  AEPRKMSIYREEAVETLIEALWQKDFSNTQMKALDALLFLIGHISSSGKSYTEAWLLKIA 1041

Query: 989  GLTKGHRKPIRRDNLSSALGEIELTRLEQKDEDKAANEWERKMAFVLVSHDFGLLLESLA 1048
            G  + +   ++ + L     ++  T    +DE  A N W++++A VL +H+ G + ++L 
Sbjct: 1042 GFDQPYNALMKVEQLGQHDNDLIET---MEDEKNALNSWQKRIASVLCNHENGSIFKALE 1101

Query: 1049 KGLKSKYAALFSACFVSATWLSHMLRTLPDTGILEAARVFLLDHFVSIFTTTTDIEEKAL 1108
            + LKS    +  +C V ATWL+ ML TLPDTG+ + AR  LL+  + +  ++  +E+  L
Sbjct: 1102 ECLKSNSLKMAKSCLVLATWLTRMLYTLPDTGVRDVARKSLLEEVIKVLHSSKSLEDMIL 1161

Query: 1109 GLLAINSFVHEPEGLQCLSSNMKNIMRGLRELKRSTPLAFDMLKVLCE-GQDSSTELWSH 1168
              L++  F+ +P   + L    K+I R LR+LK+ + +A D+LK L        TELWS 
Sbjct: 1162 VTLSLYPFISDPTVHEVLRVYAKSIYRILRKLKKYSTVAADILKALLNLNSVDVTELWSC 1221

Query: 1169 RELFQADCRKNGEVLSIAHFKGKIISGHSDGTIKVWSVRGTNLHVLQEIQEHSKGVTSLA 1228
            +E+ + D   NGEVLS+ +  G+++SG  DGT KV   R     V+QE  EH+K VTSL 
Sbjct: 1222 KEVVELDLSSNGEVLSLHYLNGQVLSGLMDGTSKVCDARKRIPRVIQETHEHTKAVTSLC 1281

Query: 1229 VSESEEKLYSGSLDKTIKIWSLGSDIIQCIQVHNVKDQIHNLVISKTVACFIPHGAGIRV 1288
               S ++LYS SLDKTI++W++ SD I+CI V+++K+ +H L  +  +AC++  G G++V
Sbjct: 1282 --SSGDRLYSASLDKTIRVWTIKSDGIKCIDVYDIKEAVHELAANDKLACYVSQGTGVKV 1341

Query: 1289 YSWDGESKVLNSSKYVKCLNLVHGKLYCGCHDGSIQEVDLATGTVSYIHSGSRKLLGKAN 1348
            ++W    K++N SKYVK L +   KLYCGC   SIQEVDL+T T +   +G+RKLLGK  
Sbjct: 1342 FNWSEAPKLINFSKYVKSLAVAGDKLYCGCSGYSIQEVDLSTYTSNSFFTGTRKLLGK-Q 1401

Query: 1349 PVQALQVYDEQLFSASTALDGAAVKIWSTSNYGVIGSLATAMDVRTMAVGINLTYLGGKG 1397
             + +LQ++D+ LF+  +++D  A KI+S S   V+GSL+T +D+  +A+  +  + G K 
Sbjct: 1402 TIHSLQIHDDYLFACVSSVDATAGKIFSLSQKMVVGSLSTGLDIHRIAINSDFIFAGTKF 1461

BLAST of Lag0034451 vs. ExPASy Swiss-Prot
Match: Q8GUG9 (U-box domain-containing protein 11 OS=Arabidopsis thaliana OX=3702 GN=PUB11 PE=2 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 2.3e-18
Identity = 104/437 (23.80%), Postives = 196/437 (44.85%), Query Frame = 0

Query: 388 QDDSSESFSLGSETEIEENDHETTLFGSEEPKPETQKLEQTIT-SMEESITQSVELD--F 447
           +D  S    + +E ++ E+  ET  FG EE K  +  L ++ + S+   +++  + D   
Sbjct: 165 RDGFSNVIKIKAEEKL-ESVSETLHFGEEEEKQSSPPLRRSSSISLAYYLSKDADTDRLD 224

Query: 448 AMEDSGNASPESGGRHNTPKDFVCPITCNIFYDPVTVETGQTYERSAIQEWLDRGNSTCP 507
            M +      +   +   P DF+CP++  +  DPV V TGQTYER+ IQ W+D GN TCP
Sbjct: 225 KMVNKNTDESKKSDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCP 284

Query: 508 ITGQKLQNTQLPKTNYVLKRLIASW-----LEENPNFVSEKALDEADPVAVLASPVSVSS 567
            T QKL+N  L   NYVL+ LI+ W     +E+   +++ +  +  D   + A    +SS
Sbjct: 285 KTQQKLENFTL-TPNYVLRSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLSS 344

Query: 568 QASIDK--SMKEVRL-------------------AITNLCASGVLEEAETAVLCVERFWL 627
           +++ D+  ++ E+R                     + NL  S  +   E A+ CV    +
Sbjct: 345 RSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENAITCVLNLSI 404

Query: 628 EENVEGDVQHMLLKPSVINGLVEILVNSV--NRQVLRAAIFLLSELGSKDDAVVQTLTRV 687
            EN     + +++    +  +V++L       R+   A +F LS L  ++  ++     +
Sbjct: 405 YEN----NKELIMFAGAVTSIVQVLRAGTMEARENAAATLFSLS-LADENKIIIGGSGAI 464

Query: 688 ESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLSHK 747
            + VD +     RG  +A   ++ L +           G + ++VK    D  + R+  +
Sbjct: 465 PALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKML-SDSTRHRMVDE 524

Query: 748 SAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQDG 794
           +  +L    +L  +++    +V     N   +++G L+  Q   R  A  ILL   ++D 
Sbjct: 525 ALTIL---SVLANNQDAKSAIVKA---NTLPALIGILQTDQTRNRENAAAILLSLCKRDT 584

BLAST of Lag0034451 vs. ExPASy Swiss-Prot
Match: Q681N2 (U-box domain-containing protein 15 OS=Arabidopsis thaliana OX=3702 GN=PUB15 PE=2 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 2.1e-16
Identity = 94/344 (27.33%), Postives = 158/344 (45.93%), Query Frame = 0

Query: 463 PKDFVCPITCNIFYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVL 522
           P +F+CPIT  I  DPV + TGQTYE+ +IQ+W D G+ TCP T Q+L +  L   N+ L
Sbjct: 291 PHEFLCPITLEIMLDPVIIATGQTYEKESIQKWFDAGHKTCPKTRQELDHLSL-APNFAL 350

Query: 523 KRLIASWLEENPNFVSEKAL------DEADPVAVLASPVSVSSQASIDKSMKEVRL---- 582
           K LI  W E+N   + EK +      ++ D V++L   +S S      +S+K++RL    
Sbjct: 351 KNLIMQWCEKNNFKIPEKEVSPDSQNEQKDEVSLLVEALSSSQLEEQRRSVKQMRLLARE 410

Query: 583 ------AITNLCA------------SGVLEEAETAVLCVERFWLEENVEGDVQHMLLKPS 642
                  I N  A            SG+ E A T +L +       +++   + ++    
Sbjct: 411 NPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTLLNL-------SIDEVNKKLISNEG 470

Query: 643 VINGLVEILVNSVN--RQVLRAAIFLLSELGSKDDAVVQTLTRVESDVDCIVTLFKRGLV 702
            I  ++EIL N     R+   AA+F LS L  ++   +     +   VD +     RG  
Sbjct: 471 AIPNIIEILENGNREARENSAAALFSLSML-DENKVTIGLSNGIPPLVDLLQHGTLRGKK 530

Query: 703 EAVVLIYQLGLSSQT---LKEMDMVGSLLSVVKKK-----EEDVNKM------------- 755
           +A+  ++ L L+S       +  +V  LL+++K K     +E ++ +             
Sbjct: 531 DALTALFNLSLNSANKGRAIDAGIVQPLLNLLKDKNLGMIDEALSILLLLASHPEGRQAI 590

BLAST of Lag0034451 vs. ExPASy TrEMBL
Match: A0A6J1F0S1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111 PE=4 SV=1)

HSP 1 Score: 2132.8 bits (5525), Expect = 0.0e+00
Identity = 1127/1342 (83.98%), Postives = 1210/1342 (90.16%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATGT TAS+ILHH SAFI+EILAQS+LRKRLLS VYG +SLSDEIALKPL LAAEALE
Sbjct: 1    MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLSLPEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF+EQRS+ILT  PSNL  N+TE+SI DES VF C
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKW EME+I E+K RTEQL LPNGRYNPIW+EEE+   +L+VD T KSK      PP V
Sbjct: 241  NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQ---TLQVDCTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEGDVQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLLSVVKKKEEDVNKMRLS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVE RIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS DEQFEIIMFLSELVKLNRRT NE+IL+NIKDG  
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSK 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMD LISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRF+TSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIE KAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSVRGTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQ H+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQAHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKIDTLQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1323

BLAST of Lag0034451 vs. ExPASy TrEMBL
Match: A0A6J1L0K6 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499948 PE=4 SV=1)

HSP 1 Score: 2127.8 bits (5512), Expect = 0.0e+00
Identity = 1123/1342 (83.68%), Postives = 1210/1342 (90.16%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATG  TAS+ILHH SAFI+EILAQS+LRKRLLS VYG ISLSDEIALKPL LAAEALE
Sbjct: 1    MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLS+PEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF++QR +ILT  PSNL  N+TE+SI DE  VFPC
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKWKEME+I ++K RTEQL  PNGRYNPIW+EEE+   +LEVD T KSK      PP V
Sbjct: 241  NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQ---TLEVDFTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEG+VQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGNVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLL+VVKKKEEDVNKM LS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVEERIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS  EQFEIIMFLSELVKLNRRT NE+IL+NIKDG +
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNGEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSE 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMDVLISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRFSTSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIEEKAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSV+GTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVKGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQVH+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQVHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKID LQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1323

BLAST of Lag0034451 vs. ExPASy TrEMBL
Match: A0A6J1F5V1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111 PE=4 SV=1)

HSP 1 Score: 2126.3 bits (5508), Expect = 0.0e+00
Identity = 1126/1342 (83.90%), Postives = 1209/1342 (90.09%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATGT TAS+ILHH SAFI+EILAQS+LRKRLLS VYG +SLSDEIALKPL LAAEALE
Sbjct: 1    MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLSLPEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF+EQRS+ILT  PSNL  N+TE+SI DES VF C
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKW EME+I E+K RTEQL LPNGRYNPIW+EEE+   +L+VD T KSK      PP V
Sbjct: 241  NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQ---TLQVDCTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEGDVQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLLSVVKKKEEDVNKMRLS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVE RIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS DEQFEIIMFLSELVKLN RT NE+IL+NIKDG  
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSK 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMD LISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRF+TSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIE KAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSVRGTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQ H+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQAHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKIDTLQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1322

BLAST of Lag0034451 vs. ExPASy TrEMBL
Match: A0A6J1L0L5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111499948 PE=4 SV=1)

HSP 1 Score: 2121.3 bits (5495), Expect = 0.0e+00
Identity = 1122/1342 (83.61%), Postives = 1209/1342 (90.09%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATG  TAS+ILHH SAFI+EILAQS+LRKRLLS VYG ISLSDEIALKPL LAAEALE
Sbjct: 1    MVATGAITASSILHHISAFISEILAQSELRKRLLSVVYGIISLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLS+PEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSVPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF++QR +ILT  PSNL  N+TE+SI DE  VFPC
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHQQRFQILTFFPSNLINNTTEHSIGDEPEVFPC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAEHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKWKEME+I ++K RTEQL  PNGRYNPIW+EEE+   +LEVD T KSK      PP V
Sbjct: 241  NEKWKEMEQIAKQKLRTEQLCFPNGRYNPIWIEEEQ---TLEVDFTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE EENDHE TLF S
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEFEENDHEKTLFDS 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             +PKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  GQPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEG+VQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGNVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLL+VVKKKEEDVNKM LS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDIVGSLLNVVKKKEEDVNKMHLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVEERIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEERIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS  EQFEIIMFLSELVKLN RT NE+IL+NIKDG +
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNGEQFEIIMFLSELVKLN-RTSNEKILRNIKDGSE 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMDVLISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDVLISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRFSTSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFSTSGQPLARYFLLERAGLTKNHRKPIGTENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIEEKAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEEKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSV+GTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVKGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQVH+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQVHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKID LQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDALQIGRNCKVVCMS 1320

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1322

BLAST of Lag0034451 vs. ExPASy TrEMBL
Match: A0A6J1F0V1 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111441111 PE=4 SV=1)

HSP 1 Score: 2108.2 bits (5461), Expect = 0.0e+00
Identity = 1118/1342 (83.31%), Postives = 1200/1342 (89.42%), Query Frame = 0

Query: 56   MVATGTTTASNILHHTSAFIAEILAQSDLRKRLLSAVYGKISLSDEIALKPLWLAAEALE 115
            MVATGT TAS+ILHH SAFI+EILAQS+LRKRLLS VYG +SLSDEIALKPL LAAEALE
Sbjct: 1    MVATGTITASSILHHISAFISEILAQSELRKRLLSVVYGIVSLSDEIALKPLELAAEALE 60

Query: 116  NAISADNSSIKSSSLRCAEELLLSLPENPFSSFLLSLIYGLNHQNLNSALSLLDLFLSDP 175
            NAI  DNSSIKSSSLRCAEELLLSLPEN  SSFLLSLIYGLNHQNLNSAL+LL LFLSDP
Sbjct: 61   NAIFTDNSSIKSSSLRCAEELLLSLPENACSSFLLSLIYGLNHQNLNSALNLLHLFLSDP 120

Query: 176  SSARSEIAPILFEELFLRHFLPIFHWFNEQRSKILTSLPSNLNQNSTEYSIDDESVVFPC 235
            S ARSEIAPILFEELFLRHFLPI HWF+EQRS+ILT  PSNL  N+TE+SI DES VF C
Sbjct: 121  SLARSEIAPILFEELFLRHFLPIIHWFHEQRSEILTFFPSNLINNTTEHSIGDESEVFHC 180

Query: 236  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAGHFKRVLERKDDGGTIANPKVRLLEK 295
            TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFA HFKRVLERKD+GG+IA P V+LL+K
Sbjct: 181  TKSLSKLSIDQTLKLKELESNYERVLDKNCRVFAKHFKRVLERKDNGGSIAIPMVKLLDK 240

Query: 296  KEKWKEMEEITEEKFRTEQLSLPNGRYNPIWVEEEEQIISLEVDSTTKSKLPRPPPPPSV 355
             EKW EME+I E+K RTEQL LPNGRYNPIW+EEE+   +L+VD T KSK      PP V
Sbjct: 241  NEKWNEMEQIAEQKLRTEQLCLPNGRYNPIWIEEEQ---TLQVDCTNKSK---SASPPCV 300

Query: 356  HSQILGPFNFLSEQESSSRSITFSIFNPAAEAQDDSSESFSLGSETEIEENDHETTLFGS 415
             SQ+ GPFN +SEQESSS S TFSIF   A+ QDDSSE FSLGSETE             
Sbjct: 301  RSQVFGPFNLISEQESSSSSRTFSIFKSTAQDQDDSSEIFSLGSETEF------------ 360

Query: 416  EEPKPETQKLEQTITSMEESITQSVELDFAMEDSGNASPESGGRHNT-PKDFVCPITCNI 475
             EPKP+ QKL+QT       ITQS+ELD+AME+S +ASPESG +H T PKDFVCPITCNI
Sbjct: 361  -EPKPKNQKLKQT-------ITQSLELDYAMENSRSASPESGEKHITPPKDFVCPITCNI 420

Query: 476  FYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 535
            FYDPVT+ETGQTYERSAIQEWLDRGN TCPITGQKLQNTQLPKTNYVLKRLIASWLEENP
Sbjct: 421  FYDPVTIETGQTYERSAIQEWLDRGNLTCPITGQKLQNTQLPKTNYVLKRLIASWLEENP 480

Query: 536  NFVSEKALDEADPVAVLASPVSVSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVE 595
            NFV +KALDEA+ VAVL SPVS  SQASID+SM E+R AITNLC S VLEEAETAVLCVE
Sbjct: 481  NFVLDKALDEAESVAVLTSPVSFISQASIDRSM-EMRRAITNLCTSEVLEEAETAVLCVE 540

Query: 596  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLT 655
            RFWLEENVEGDVQHMLLKPSVINGLVEILVNSV+ QVLRAAIFLLSELG K D V++TLT
Sbjct: 541  RFWLEENVEGDVQHMLLKPSVINGLVEILVNSVDLQVLRAAIFLLSELGFKGDIVIETLT 600

Query: 656  RVESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLS 715
            RVESDV+CIVTLFKRG+VEAV+LIYQLGLSSQTL+EMD+VGSLLSVVKKKEEDVNKMRLS
Sbjct: 601  RVESDVNCIVTLFKRGVVEAVLLIYQLGLSSQTLQEMDVVGSLLSVVKKKEEDVNKMRLS 660

Query: 716  HKSAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQ 775
            HKSAAVLLLRKIL  SKEGSP VV+VLAENA ESILGSLKAKQVE RIAAVGILLRCIQ+
Sbjct: 661  HKSAAVLLLRKIL-GSKEGSPTVVAVLAENAIESILGSLKAKQVEVRIAAVGILLRCIQE 720

Query: 776  DGKCRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGD 835
            D KCRNIIA KADLA IL SF +VS DEQFEIIMFLSELVKLNRRT NE+IL+NIKDG  
Sbjct: 721  DWKCRNIIADKADLALILSSFTKVSNDEQFEIIMFLSELVKLNRRTSNEKILRNIKDGSK 780

Query: 836  CSTMHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPT 895
            CSTMHSLL YLQTA RDQCPVVAGLLLQLD LVEP KMSIYREEAMD LISCLRDS+FPT
Sbjct: 781  CSTMHSLLTYLQTAHRDQCPVVAGLLLQLDILVEPLKMSIYREEAMDALISCLRDSDFPT 840

Query: 896  AQISAAETIMSLQGRFSTSGRPLARYFLLERAGLTKGHRKPIRRDNLSSALGEIELTRLE 955
            AQISAAETIMSLQGRF+TSG+PLARYFLLERAGLTK HRKPI  +N+S ALGE+ELTR  
Sbjct: 841  AQISAAETIMSLQGRFNTSGQPLARYFLLERAGLTKNHRKPIGSENISDALGEVELTR-- 900

Query: 956  QKDEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTL 1015
              +EDKA +EWERKMAFVLV+HDFGLLLE LAKGLKSK AALFSACFVSATWLSHML TL
Sbjct: 901  --EEDKATDEWERKMAFVLVNHDFGLLLEPLAKGLKSKCAALFSACFVSATWLSHMLGTL 960

Query: 1016 PDTGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRG 1075
            PDTGILEAAR+FLLDHF+SIFT TTDIE KAL LLAINSFVHEPEGLQ LSS+MKNIMRG
Sbjct: 961  PDTGILEAARIFLLDHFLSIFTMTTDIEGKALALLAINSFVHEPEGLQYLSSDMKNIMRG 1020

Query: 1076 LRELKRSTPLAFDMLKVLCEGQDSSTELWSHRELFQADCRKNGEVLSIAHFKGKIISGHS 1135
            L+E +RSTPLAFDMLKVLCEGQDSSTELWSH ELF+ DC +NGEVLSIAH K +IISGHS
Sbjct: 1021 LKEFRRSTPLAFDMLKVLCEGQDSSTELWSHHELFKVDCSRNGEVLSIAHCKDRIISGHS 1080

Query: 1136 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1195
            DGT+KVWSVRGTNLH+LQEIQEHSKGVTSLAV+ESEEKLYSGSLDKTIK+WSLGSDI+QC
Sbjct: 1081 DGTMKVWSVRGTNLHLLQEIQEHSKGVTSLAVAESEEKLYSGSLDKTIKVWSLGSDIVQC 1140

Query: 1196 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1255
            IQ H+VK+QIHNLV+SK+VACFIPHG GIRVYSW GESK+LNSSK+VKCLNL+HGKLYCG
Sbjct: 1141 IQAHDVKEQIHNLVVSKSVACFIPHGVGIRVYSWAGESKLLNSSKHVKCLNLMHGKLYCG 1200

Query: 1256 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1315
            CHD SIQEVDLATGTVSYIHSGSRKLLGKANP+ ALQVYDEQLFSASTALDGAAVKI   
Sbjct: 1201 CHDSSIQEVDLATGTVSYIHSGSRKLLGKANPIHALQVYDEQLFSASTALDGAAVKIRCR 1260

Query: 1316 SNYGVIGSLATAMDVRTMAVGINLTYLGGKGGVVEIWGREKHNKIDTLQIGRNCKVVCMT 1375
            SNYGVIGSL+TAMDVRTMAVG NLTYLGGKGGVVEIW REKHNKIDTLQIGRNCKVVCM+
Sbjct: 1261 SNYGVIGSLSTAMDVRTMAVGSNLTYLGGKGGVVEIWSREKHNKIDTLQIGRNCKVVCMS 1310

Query: 1376 LDEREEVLVAGTSDGQIQAWGL 1397
            L+ERE+VLV GTSDG+IQAW L
Sbjct: 1321 LNEREDVLVIGTSDGRIQAWEL 1310

BLAST of Lag0034451 vs. TAIR 10
Match: AT3G06880.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 232.3 bits (591), Expect = 2.4e-60
Identity = 203/804 (25.25%), Postives = 370/804 (46.02%), Query Frame = 0

Query: 607  QHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLTRVESDVDCIVTL 666
            ++ +LK  +++ L   + +S  + V++A++  L+++ S +   ++ + R   ++  +   
Sbjct: 478  KYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIISVNRTALEEVKRKGLNLSHLANA 537

Query: 667  FKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEED------VNKMRLSHKSAAV 726
             K+ + EA +LIY +  S   +K ++++ +L+ VV            +         AA 
Sbjct: 538  LKQNVQEAAILIYLIKPSPTEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAAS 597

Query: 727  LLLRKILERSKEGSPIVVSVLAENATESILGSL---KAKQVEERIAAVGILLRCIQQDGK 786
            L++ ++L  + + +   + + A ++   + G L   K+    E I+   IL++C+Q DG 
Sbjct: 598  LMIIEVLITAFDHATNTMHLAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGL 657

Query: 787  CRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGDCST 846
             R  I     +AP         ++E    + FL E++K+ R +   +ILQ IK  G    
Sbjct: 658  HRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLHEVLKIPRSS-AIKILQQIKKEGSFDI 717

Query: 847  MHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPTAQI 906
              +LL  ++    D     A +LLQL+ L  P +   YR EA   L+  +  SE    Q+
Sbjct: 718  KVTLLHCIKHLQGDHKLFAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQL 777

Query: 907  SAAETIMSLQGRFSTSGRPLARYFLLERAGLTK-GHRKPIRRDNLSSALGEIELTRLEQK 966
             +   + ++ G +S +G P    +L++R GLT   H   IR  N S           ++ 
Sbjct: 778  LSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMSHMNMIRNINWS-----------DEC 837

Query: 967  DEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTLPD 1026
             +D   + W  K+A  ++          L +GLKSK  ++  AC ++  WLS  +   P+
Sbjct: 838  LQDTGIDGWCCKIARRIIDTGKATFC-GLQEGLKSKNKSVSKACLIAIAWLSIEISKGPN 897

Query: 1027 TGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRGLR 1086
            +    A  V LLD          ++EE+ L  + I +F    +G+  L +  + +   LR
Sbjct: 898  SLKYSACEV-LLDEVAQFLHPGLELEERLLACICIYNF-SSGKGIHKLVNFSEGVRESLR 957

Query: 1087 ELKRSTPLAFDMLKVLCEGQDSSTELWS--HRELFQADCRKNGEVLSIAHFKGKIISGHS 1146
             L   T +A ++ K        S +  S  H +  +     +G V ++ + KG + SG S
Sbjct: 958  RLSHVTWMADELHKATYYLFSKSDQRISCVHTQTVEMHQSGSGAVTALIYHKGLLFSGFS 1017

Query: 1147 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1206
            DG+I+VW+V      +L +I+EH   VT  ++SE+ E + SGS DKTI++W +    ++C
Sbjct: 1018 DGSIRVWNVNKKIATLLWDIKEHKSTVTCFSLSETGECVLSGSADKTIRVWQIVKGKLEC 1077

Query: 1207 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1266
             +V   KD I  L     +   I  G  +++      S+ +   K VK +    GK+Y G
Sbjct: 1078 AEVIKTKDSIRKLEAFGNMIFVITKGHKMKLLDSSRISQSIFKGKGVKSMVSAQGKIYIG 1137

Query: 1267 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1326
            C D SIQE+ +A      I + +R    +  P+ ++ VY + L+S+ST ++ + +K    
Sbjct: 1138 CIDTSIQELIVANKREKEIKAPTRSWRLQNKPINSVVVYKDMLYSSSTYVEMSNIKD-LR 1197

Query: 1327 SNYGVIGSLATAMDVRTMAVGI--NLTYL--GGKGGVVEIWGREKHNKIDTLQIGRNCKV 1386
             NY    S+        +A+G+  +  YL        ++IW R    K+  L  G     
Sbjct: 1198 RNYEPQMSITAEKGSNIVAMGVVEDFIYLNRSSSANTLQIWLRRTQQKVGRLSAGSK--- 1257

Query: 1387 VCMTLDEREEVLVAGTSDGQIQAW 1395
               +L    +++  GT  G I+ W
Sbjct: 1258 -ITSLLTANDIVFCGTEAGVIKGW 1261

BLAST of Lag0034451 vs. TAIR 10
Match: AT3G06880.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 226.5 bits (576), Expect = 1.3e-58
Identity = 201/799 (25.16%), Postives = 367/799 (45.93%), Query Frame = 0

Query: 607  QHMLLKPSVINGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLTRVESDVDCIVTL 666
            ++ +LK  +++ L   + +S  + V++A++  L+++ S +   ++ + R   ++  +   
Sbjct: 478  KYSMLKDVILDQLFTAISSSEEKTVIKASMTALTKIISVNRTALEEVKRKGLNLSHLANA 537

Query: 667  FKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEED------VNKMRLSHKSAAV 726
             K+ + EA +LIY +  S   +K ++++ +L+ VV            +         AA 
Sbjct: 538  LKQNVQEAAILIYLIKPSPTEIKSLELLPALVDVVASTSSSSSCYTFIPSPSYLTPPAAS 597

Query: 727  LLLRKILERSKEGSPIVVSVLAENATESILGSL---KAKQVEERIAAVGILLRCIQQDGK 786
            L++ ++L  + + +   + + A ++   + G L   K+    E I+   IL++C+Q DG 
Sbjct: 598  LMIIEVLITAFDHATNTMHLAAISSPSVLCGLLDVAKSGNSGEFISLTSILVKCMQFDGL 657

Query: 787  CRNIIAVKADLAPILESFAEVSKDEQFEIIMFLSELVKLNRRTFNEQILQNIKDGGDCST 846
             R  I     +AP         ++E    + FL E++K+ R +   +ILQ IK  G    
Sbjct: 658  HRKYIYQHTRVAPFAHLLQSKDQEEICIALQFLHEVLKIPRSS-AIKILQQIKKEGSFDI 717

Query: 847  MHSLLIYLQTAIRDQCPVVAGLLLQLDNLVEPQKMSIYREEAMDVLISCLRDSEFPTAQI 906
              +LL  ++    D     A +LLQL+ L  P +   YR EA   L+  +  SE    Q+
Sbjct: 718  KVTLLHCIKHLQGDHKLFAADILLQLNALDSPPENKKYRNEATRALLDAVTYSEGSNMQL 777

Query: 907  SAAETIMSLQGRFSTSGRPLARYFLLERAGLTK-GHRKPIRRDNLSSALGEIELTRLEQK 966
             +   + ++ G +S +G P    +L++R GLT   H   IR  N S           ++ 
Sbjct: 778  LSTFILANIGGTYSWTGEPYTAAWLMKRGGLTSMSHMNMIRNINWS-----------DEC 837

Query: 967  DEDKAANEWERKMAFVLVSHDFGLLLESLAKGLKSKYAALFSACFVSATWLSHMLRTLPD 1026
             +D   + W  K+A  ++          L +GLKSK  ++  AC ++  WLS  +   P+
Sbjct: 838  LQDTGIDGWCCKIARRIIDTGKATFC-GLQEGLKSKNKSVSKACLIAIAWLSIEISKGPN 897

Query: 1027 TGILEAARVFLLDHFVSIFTTTTDIEEKALGLLAINSFVHEPEGLQCLSSNMKNIMRGLR 1086
            +    A  V LLD          ++EE+ L  + I +F    +G+  L +  + +   LR
Sbjct: 898  SLKYSACEV-LLDEVAQFLHPGLELEERLLACICIYNF-SSGKGIHKLVNFSEGVRESLR 957

Query: 1087 ELKRSTPLAFDMLKVLCEGQDSSTELWS--HRELFQADCRKNGEVLSIAHFKGKIISGHS 1146
             L   T +A ++ K        S +  S  H +  +     +G V ++ + KG + SG S
Sbjct: 958  RLSHVTWMADELHKATYYLFSKSDQRISCVHTQTVEMHQSGSGAVTALIYHKGLLFSGFS 1017

Query: 1147 DGTIKVWSVRGTNLHVLQEIQEHSKGVTSLAVSESEEKLYSGSLDKTIKIWSLGSDIIQC 1206
            DG+I+VW+V      +L +I+EH   VT  ++SE+ E + SGS DKTI++W +    ++C
Sbjct: 1018 DGSIRVWNVNKKIATLLWDIKEHKSTVTCFSLSETGECVLSGSADKTIRVWQIVKGKLEC 1077

Query: 1207 IQVHNVKDQIHNLVISKTVACFIPHGAGIRVYSWDGESKVLNSSKYVKCLNLVHGKLYCG 1266
             +V   KD I  L     +   I  G  +++      S+ +   K VK +    GK+Y G
Sbjct: 1078 AEVIKTKDSIRKLEAFGNMIFVITKGHKMKLLDSSRISQSIFKGKGVKSMVSAQGKIYIG 1137

Query: 1267 CHDGSIQEVDLATGTVSYIHSGSRKLLGKANPVQALQVYDEQLFSASTALDGAAVKIWST 1326
            C D SIQE+ +A      I + +R    +  P+ ++ VY + L+S+ST ++ + +K    
Sbjct: 1138 CIDTSIQELIVANKREKEIKAPTRSWRLQNKPINSVVVYKDMLYSSSTYVEMSNIKD-LR 1197

Query: 1327 SNYGVIGSLATAMDVRTMAVGI--NLTYL--GGKGGVVEIWGREKHNKIDTLQIGRNCKV 1386
             NY    S+        +A+G+  +  YL        ++IW R    K+  L  G     
Sbjct: 1198 RNYEPQMSITAEKGSNIVAMGVVEDFIYLNRSSSANTLQIWLRRTQQKVGRLSAGSK--- 1256

Query: 1387 VCMTLDEREEVLVAGTSDG 1390
               +L    +++  GT  G
Sbjct: 1258 -ITSLLTANDIVFCGTEAG 1256

BLAST of Lag0034451 vs. TAIR 10
Match: AT1G23030.1 (ARM repeat superfamily protein )

HSP 1 Score: 96.7 bits (239), Expect = 1.6e-19
Identity = 104/437 (23.80%), Postives = 196/437 (44.85%), Query Frame = 0

Query: 388 QDDSSESFSLGSETEIEENDHETTLFGSEEPKPETQKLEQTIT-SMEESITQSVELD--F 447
           +D  S    + +E ++ E+  ET  FG EE K  +  L ++ + S+   +++  + D   
Sbjct: 165 RDGFSNVIKIKAEEKL-ESVSETLHFGEEEEKQSSPPLRRSSSISLAYYLSKDADTDRLD 224

Query: 448 AMEDSGNASPESGGRHNTPKDFVCPITCNIFYDPVTVETGQTYERSAIQEWLDRGNSTCP 507
            M +      +   +   P DF+CP++  +  DPV V TGQTYER+ IQ W+D GN TCP
Sbjct: 225 KMVNKNTDESKKSDKLTIPVDFLCPVSLELMKDPVIVATGQTYERAYIQRWIDCGNLTCP 284

Query: 508 ITGQKLQNTQLPKTNYVLKRLIASW-----LEENPNFVSEKALDEADPVAVLASPVSVSS 567
            T QKL+N  L   NYVL+ LI+ W     +E+   +++ +  +  D   + A    +SS
Sbjct: 285 KTQQKLENFTL-TPNYVLRSLISRWCAEHNIEQPAGYINGRTKNSGDMSVIRALVQRLSS 344

Query: 568 QASIDK--SMKEVRL-------------------AITNLCASGVLEEAETAVLCVERFWL 627
           +++ D+  ++ E+R                     + NL  S  +   E A+ CV    +
Sbjct: 345 RSTEDRRNAVSEIRSLSKRSTDNRILIAEAGAIPVLVNLLTSEDVATQENAITCVLNLSI 404

Query: 628 EENVEGDVQHMLLKPSVINGLVEILVNSV--NRQVLRAAIFLLSELGSKDDAVVQTLTRV 687
            EN     + +++    +  +V++L       R+   A +F LS L  ++  ++     +
Sbjct: 405 YEN----NKELIMFAGAVTSIVQVLRAGTMEARENAAATLFSLS-LADENKIIIGGSGAI 464

Query: 688 ESDVDCIVTLFKRGLVEAVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLSHK 747
            + VD +     RG  +A   ++ L +           G + ++VK    D  + R+  +
Sbjct: 465 PALVDLLENGTPRGKKDAATALFNLCIYHGNKGRAVRAGIVTALVKML-SDSTRHRMVDE 524

Query: 748 SAAVLLLRKILERSKEGSPIVVSVLAENATESILGSLKAKQVEERIAAVGILLRCIQQDG 794
           +  +L    +L  +++    +V     N   +++G L+  Q   R  A  ILL   ++D 
Sbjct: 525 ALTIL---SVLANNQDAKSAIVKA---NTLPALIGILQTDQTRNRENAAAILLSLCKRDT 584

BLAST of Lag0034451 vs. TAIR 10
Match: AT5G42340.1 (Plant U-Box 15 )

HSP 1 Score: 90.1 bits (222), Expect = 1.5e-17
Identity = 94/344 (27.33%), Postives = 158/344 (45.93%), Query Frame = 0

Query: 463 PKDFVCPITCNIFYDPVTVETGQTYERSAIQEWLDRGNSTCPITGQKLQNTQLPKTNYVL 522
           P +F+CPIT  I  DPV + TGQTYE+ +IQ+W D G+ TCP T Q+L +  L   N+ L
Sbjct: 291 PHEFLCPITLEIMLDPVIIATGQTYEKESIQKWFDAGHKTCPKTRQELDHLSL-APNFAL 350

Query: 523 KRLIASWLEENPNFVSEKAL------DEADPVAVLASPVSVSSQASIDKSMKEVRL---- 582
           K LI  W E+N   + EK +      ++ D V++L   +S S      +S+K++RL    
Sbjct: 351 KNLIMQWCEKNNFKIPEKEVSPDSQNEQKDEVSLLVEALSSSQLEEQRRSVKQMRLLARE 410

Query: 583 ------AITNLCA------------SGVLEEAETAVLCVERFWLEENVEGDVQHMLLKPS 642
                  I N  A            SG+ E A T +L +       +++   + ++    
Sbjct: 411 NPENRVLIANAGAIPLLVQLLSYPDSGIQENAVTTLLNL-------SIDEVNKKLISNEG 470

Query: 643 VINGLVEILVNSVN--RQVLRAAIFLLSELGSKDDAVVQTLTRVESDVDCIVTLFKRGLV 702
            I  ++EIL N     R+   AA+F LS L  ++   +     +   VD +     RG  
Sbjct: 471 AIPNIIEILENGNREARENSAAALFSLSML-DENKVTIGLSNGIPPLVDLLQHGTLRGKK 530

Query: 703 EAVVLIYQLGLSSQT---LKEMDMVGSLLSVVKKK-----EEDVNKM------------- 755
           +A+  ++ L L+S       +  +V  LL+++K K     +E ++ +             
Sbjct: 531 DALTALFNLSLNSANKGRAIDAGIVQPLLNLLKDKNLGMIDEALSILLLLASHPEGRQAI 590

BLAST of Lag0034451 vs. TAIR 10
Match: AT1G71020.1 (ARM repeat superfamily protein )

HSP 1 Score: 84.0 bits (206), Expect = 1.1e-15
Identity = 91/361 (25.21%), Postives = 166/361 (45.98%), Query Frame = 0

Query: 442 LDFAMEDSGNASPESGGRHNTPKDFVCPITCNIFYDPVTVETGQTYERSAIQEWLDRGNS 501
           L+ A+ ++ + S +S      P+DF+CPI+  +  DP  V TGQTYERS IQ W+D GN 
Sbjct: 224 LEKAVTENSDDSQKSDNL-TIPEDFLCPISLELMKDPAIVSTGQTYERSFIQRWIDCGNL 283

Query: 502 TCPITGQKLQNTQLPKTNYVLKRLIASW-----LEENPNFVSEKALDEADPVAVLASPVS 561
           +CP T QKL+N  L   NYVL+ LI+ W     +E+   +++ +  +            S
Sbjct: 284 SCPKTQQKLENFTL-TPNYVLRSLISQWCTKHNIEQPGGYMNGRTKN------------S 343

Query: 562 VSSQASIDKSMKEVRLAITNLCASGVLEEAETAVLCVERFWLEENVEGDVQHMLLKPSVI 621
             S   +   M  +R  +  L +S  +E+  TAV  +           D + ++ +   I
Sbjct: 344 DGSFRDLSGDMSAIRALVCKL-SSQSIEDRRTAVSEIRSL---SKRSTDNRILIAEAGAI 403

Query: 622 NGLVEILVNSVNRQVLRAAIFLLSELGSKDDAVVQTLTRVESDVDCIVTLFKRGLVE--- 681
             LV++L +  + +    A+  +  L   +    + L  +   V  IV + + G +E   
Sbjct: 404 PVLVKLLTSDGDTETQENAVTCILNLSIYEHN--KELIMLAGAVTSIVLVLRAGSMEARE 463

Query: 682 -AVVLIYQLGLSSQTLKEMDMVGSLLSVVKKKEEDVNKMRLSHKSAAVLLLRKILERSKE 741
            A   ++ L L+ +    +   G+++++V   +     +R   K AA  L    + +  +
Sbjct: 464 NAAATLFSLSLADENKIIIGASGAIMALVDLLQ--YGSVR-GKKDAATALFNLCIYQGNK 523

Query: 742 GSPI-------VVSVLAENATE-------SILGSLKAKQVEE----RIAAVGILLRCIQQ 776
           G  +       +V +L ++++E       +IL  L + QV +    R  A+  L+ C+Q+
Sbjct: 524 GRAVRAGIVKPLVKMLTDSSSERMADEALTILSVLASNQVAKTAILRANAIPPLIDCLQK 561