Lag0034312 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0034312
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	mitogen-activated protein kinase kinase kinase 17-like
Location	chr3: 6262712 .. 6263710 (-)
RNA-Seq Expression	Lag0034312
Synteny	Lag0034312

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTGAGCAAAGTCGTAACTTCGTAAACTTGCACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTATGCAAAAAAGACACTGCCCGACAACAACTGGAACGACAGCGTCAAACATTACGACGGAAGATTTGCCAATCTGACCGTCGAAGAATCCCACGGTTCGGGTTGGATTGGAGACCGTCTTCTTCGTAAAACTGGCTCTGGCCTTGTCTTTTTGGCCAAGAACAGAACACCCACCAACCCAAATTCAGATCTTCCCACAGAAATCGTCGTCAAATCTTGTTTCATGGAACTCTCTTCTTCACTTCAACACGAGAAGGAAGTGCTCAGTGATTTGGCACCCTGCACTAATGTAGTACGTTGCTACGGCGATGAAATCACAACGACAAAATCCGGTGAGAAAATTTACAACGTCCTGTTCGAGTATTGCTCTGGCTCGAGCTTGCGTGACCACATCATTAAATCTGGACTTAACGGATTGCCGGAATACGAAGTTCGTAAGTATACAAGAGACATTGTTCATGGGCTCTATCACATGCATTGCAATGCTCGCTACATCCACGGCGACATAAAACCAAGAAACATCTTATTGTTACCTAGTGCTTGTGCAGCTGACAAAGGTTCTTTAATAGCTAAGATGACCAGTTTTGGATTGGCTAAGAAGCTAACTCCTGAAGTACTATGTGACCAAGAAATGAGTGGTTCGGGGCCGTACATATCTCCAGAGTTGGCAAGGGATGGCTATTTAGATTGGCCTGCTGACATTTGGGCGCTTGGATGCGTGGTGTTGGAGATGTTGACTGGAAAACCAGCTTGGAGCTTTGAGAATGCTAATAAGTACATGTTGGATTGCTACAACAACCAGCTGCTGCCAGAAATTTCGAAGGAGATTTCAAGTGCTGGAAGAGATTTTGTAAAAAGGTGTTTGATTAGAAGTCCTTACAAAAGAAGACCCATTTGGTTGTTGATCAAGCATCCGTTTGTTTGCTTGTGA

mRNA sequence

ATGAGTGAGCAAAGTCGTAACTTCGTAAACTTGCACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTATGCAAAAAAGACACTGCCCGACAACAACTGGAACGACAGCGTCAAACATTACGACGGAAGATTTGCCAATCTGACCGTCGAAGAATCCCACGGTTCGGGTTGGATTGGAGACCGTCTTCTTCGTAAAACTGGCTCTGGCCTTGTCTTTTTGGCCAAGAACAGAACACCCACCAACCCAAATTCAGATCTTCCCACAGAAATCGTCGTCAAATCTTGTTTCATGGAACTCTCTTCTTCACTTCAACACGAGAAGGAAGTGCTCAGTGATTTGGCACCCTGCACTAATGTAGTACGTTGCTACGGCGATGAAATCACAACGACAAAATCCGGTGAGAAAATTTACAACGTCCTGTTCGAGTATTGCTCTGGCTCGAGCTTGCGTGACCACATCATTAAATCTGGACTTAACGGATTGCCGGAATACGAAGTTCGTAAGTATACAAGAGACATTGTTCATGGGCTCTATCACATGCATTGCAATGCTCGCTACATCCACGGCGACATAAAACCAAGAAACATCTTATTGTTACCTAGTGCTTGTGCAGCTGACAAAGGTTCTTTAATAGCTAAGATGACCAGTTTTGGATTGGCTAAGAAGCTAACTCCTGAAGTACTATGTGACCAAGAAATGAGTGGTTCGGGGCCGTACATATCTCCAGAGTTGGCAAGGGATGGCTATTTAGATTGGCCTGCTGACATTTGGGCGCTTGGATGCGTGGTGTTGGAGATGTTGACTGGAAAACCAGCTTGGAGCTTTGAGAATGCTAATAAGTACATGTTGGATTGCTACAACAACCAGCTGCTGCCAGAAATTTCGAAGGAGATTTCAAGTGCTGGAAGAGATTTTGTAAAAAGGTGTTTGATTAGAAGTCCTTACAAAAGAAGACCCATTTGGTTGTTGATCAAGCATCCGTTTGTTTGCTTGTGA

Coding sequence (CDS)

ATGAGTGAGCAAAGTCGTAACTTCGTAAACTTGCACGATTTCTTGAAAACGAAGAGAGGCATTGTGAAGTATGCAAAAAAGACACTGCCCGACAACAACTGGAACGACAGCGTCAAACATTACGACGGAAGATTTGCCAATCTGACCGTCGAAGAATCCCACGGTTCGGGTTGGATTGGAGACCGTCTTCTTCGTAAAACTGGCTCTGGCCTTGTCTTTTTGGCCAAGAACAGAACACCCACCAACCCAAATTCAGATCTTCCCACAGAAATCGTCGTCAAATCTTGTTTCATGGAACTCTCTTCTTCACTTCAACACGAGAAGGAAGTGCTCAGTGATTTGGCACCCTGCACTAATGTAGTACGTTGCTACGGCGATGAAATCACAACGACAAAATCCGGTGAGAAAATTTACAACGTCCTGTTCGAGTATTGCTCTGGCTCGAGCTTGCGTGACCACATCATTAAATCTGGACTTAACGGATTGCCGGAATACGAAGTTCGTAAGTATACAAGAGACATTGTTCATGGGCTCTATCACATGCATTGCAATGCTCGCTACATCCACGGCGACATAAAACCAAGAAACATCTTATTGTTACCTAGTGCTTGTGCAGCTGACAAAGGTTCTTTAATAGCTAAGATGACCAGTTTTGGATTGGCTAAGAAGCTAACTCCTGAAGTACTATGTGACCAAGAAATGAGTGGTTCGGGGCCGTACATATCTCCAGAGTTGGCAAGGGATGGCTATTTAGATTGGCCTGCTGACATTTGGGCGCTTGGATGCGTGGTGTTGGAGATGTTGACTGGAAAACCAGCTTGGAGCTTTGAGAATGCTAATAAGTACATGTTGGATTGCTACAACAACCAGCTGCTGCCAGAAATTTCGAAGGAGATTTCAAGTGCTGGAAGAGATTTTGTAAAAAGGTGTTTGATTAGAAGTCCTTACAAAAGAAGACCCATTTGGTTGTTGATCAAGCATCCGTTTGTTTGCTTGTGA

Protein sequence

MSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIGDRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEISSAGRDFVKRCLIRSPYKRRPIWLLIKHPFVCL

Homology

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BLAST of Lag0034312 vs. NCBI nr
Match: XP_023516554.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 508.4 bits (1308), Expect = 4.5e-140
Identity = 243/330 (73.64%), Postives = 277/330 (83.94%), Query Frame = 0


Query: 1   MSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIG 60
           +++Q  N VNLH+FLKTKRGIVK AKK+   NNWN++VKH  GRF N  +EE HGS WIG
Sbjct: 10  VNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSNNWNNNVKHNYGRFGNFAIEEYHGSFWIG 69

Query: 61  DRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNV 120
           DRL+RK+GSGLVFLAKN++ TN N     E +VKSC ME SSSLQ+EKEVL +L PC+N+
Sbjct: 70  DRLIRKSGSGLVFLAKNKSSTNSN----PEFLVKSCLMESSSSLQYEKEVLRNLGPCSNL 129

Query: 121 VRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYH 180
           VRCYGDEIT  KSGE+IYNV+FEYC GSSLRDHIIK G NGLPE  VR+YTRDIVHGL +
Sbjct: 130 VRCYGDEITMNKSGEQIYNVVFEYCRGSSLRDHIIKFGRNGLPEPIVRQYTRDIVHGLCY 189

Query: 181 MHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPY 240
           MHCNA YIHGDIKPRNILL PS      G L+AK+ SFGLA+KLTPEVLCD+E+SGSG Y
Sbjct: 190 MHCNAGYIHGDIKPRNILLSPS--DHQGGCLVAKLASFGLARKLTPEVLCDEEISGSGSY 249

Query: 241 ISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEIS 300
           I+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y++DC  NQ +PEI  +IS
Sbjct: 250 IAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMDC--NQEIPEIPSKIS 309

Query: 301 SAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
           SAGRDFVKRCLIRSPYKRRP WLL+KHPFV
Sbjct: 310 SAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Lag0034312 vs. NCBI nr
Match: KAG7023508.1 (Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 504.2 bits (1297), Expect = 8.5e-139
Identity = 242/330 (73.33%), Postives = 276/330 (83.64%), Query Frame = 0


Query: 1   MSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIG 60
           +++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +EE HGS WIG
Sbjct: 10  VNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIEEYHGSFWIG 69

Query: 61  DRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNV 120
           DRL+RK+GSGLVFLAKN++ TN NS    E +VKSC ME SSSLQ+EKEVL +L PC+N+
Sbjct: 70  DRLIRKSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVLCNLGPCSNL 129

Query: 121 VRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYH 180
           VRCYGDEIT  KSGE+IYNV+FEYC GSSLRDHIIK G NGLPE  VRKYTRDIV GL +
Sbjct: 130 VRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCY 189

Query: 181 MHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPY 240
           MHCNA YIHGDIKPRNILL PS      G L+AK+ SFGLA+KLTPEVLCD+E+SGSG Y
Sbjct: 190 MHCNAGYIHGDIKPRNILLSPSDHKG--GCLVAKLASFGLARKLTPEVLCDEEISGSGSY 249

Query: 241 ISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEIS 300
           I+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  NQ +PEI  +IS
Sbjct: 250 IAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQEIPEIPSKIS 309

Query: 301 SAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
           SAGRDFVKRCLIRSPYKRRP WLL+KHPFV
Sbjct: 310 SAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Lag0034312 vs. NCBI nr
Match: XP_022960664.1 (mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata])

HSP 1 Score: 503.8 bits (1296), Expect = 1.1e-138
Identity = 241/330 (73.03%), Postives = 277/330 (83.94%), Query Frame = 0


Query: 1   MSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIG 60
           +++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +EE HGS WIG
Sbjct: 10  VNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIEECHGSFWIG 69

Query: 61  DRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNV 120
           DRL+R++GSGLVFLAKN++ TN NS    E +VKSC ME SSSLQ+EKEVL +L PC+N+
Sbjct: 70  DRLIRRSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVLCNLGPCSNL 129

Query: 121 VRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYH 180
           VRCYGDEIT +KSGE+IYNV+FEYC GSSLRDHIIK G NGLPE  VRKYTRDIV GL +
Sbjct: 130 VRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCY 189

Query: 181 MHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPY 240
           MHCNA YIHGDIKPRNILL PS      G L+AK+ SFGLA+KLTPEVLCD+E+SGSG Y
Sbjct: 190 MHCNAGYIHGDIKPRNILLSPSDHKG--GCLVAKLASFGLARKLTPEVLCDEEISGSGSY 249

Query: 241 ISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEIS 300
           I+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  NQ +PEI  +IS
Sbjct: 250 IAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQEIPEIPSKIS 309

Query: 301 SAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
           SAGRDFVKRCLIRSPYKRRP WLL+KHPFV
Sbjct: 310 SAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Lag0034312 vs. NCBI nr
Match: KAG6589837.1 (Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 503.1 bits (1294), Expect = 1.9e-138
Identity = 241/330 (73.03%), Postives = 276/330 (83.64%), Query Frame = 0


Query: 1   MSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIG 60
           +++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +EE HGS WIG
Sbjct: 10  VNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIEECHGSFWIG 69

Query: 61  DRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNV 120
           DRL+RK+GSGLVFLAKN++ TN NS    E +VKSC ME SSSLQ+EKEVL +L PC+N+
Sbjct: 70  DRLIRKSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVLCNLGPCSNL 129

Query: 121 VRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYH 180
           VRCYGDEIT  KSGE+IYNV+FEYC GSSLRDHIIK G NGLPE  VRKYTRDIV GL +
Sbjct: 130 VRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCY 189

Query: 181 MHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPY 240
           +HCNA YIHGDIKPRNILL PS      G L+AK+ SFGLA+KLTPEVLCD+E+SGSG Y
Sbjct: 190 IHCNAGYIHGDIKPRNILLSPS--DRKGGCLVAKLASFGLARKLTPEVLCDEEISGSGSY 249

Query: 241 ISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEIS 300
           I+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  NQ +PEI  +IS
Sbjct: 250 IAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQEIPEIPSKIS 309

Query: 301 SAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
           SAGRDFVKRCLIRSPYKRRP WLL+KHPFV
Sbjct: 310 SAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Lag0034312 vs. NCBI nr
Match: XP_038880300.1 (mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida])

HSP 1 Score: 462.6 bits (1189), Expect = 2.8e-126
Identity = 223/333 (66.97%), Postives = 260/333 (78.08%), Query Frame = 0


Query: 1   MSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFA-NLTVEESHGSGWI 60
           ++++S NFVNLH+FLKTKRGIVK AKK+L  NNWND+VKH   +F  +  +EE HGS W 
Sbjct: 10  VNQRSHNFVNLHEFLKTKRGIVKCAKKSLRTNNWNDNVKHNSEKFGKDFAIEEYHGSIWT 69

Query: 61  GDRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTN 120
           GD L RKTGS L+FLA+NR PTNPNS+LPTE +VKSC ME SSSLQHEKEVL++L   TN
Sbjct: 70  GDHLFRKTGSSLIFLARNRAPTNPNSNLPTEFMVKSCLMESSSSLQHEKEVLNNLGSFTN 129

Query: 121 VVRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLY 180
           +VRCYGDEIT TKSGEK+YNVLFEYC GSSLRDHI+K G NGLPE+EVR+YTRDIV+G+ 
Sbjct: 130 LVRCYGDEITITKSGEKVYNVLFEYCYGSSLRDHILKFGPNGLPEHEVRRYTRDIVYGVC 189

Query: 181 HMHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGP 240
           +MHCN RYIHGDIK RNIL+ P+          AK+ SFG A+KLT EVLC++E+ GSGP
Sbjct: 190 YMHCNGRYIHGDIKSRNILMSPTG---------AKLASFGWARKLTAEVLCEEEIRGSGP 249

Query: 241 YISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEI 300
           YISPELA DGYL WPADIWALGCVVLEMLTGK                NN+   +I  +I
Sbjct: 250 YISPELASDGYLGWPADIWALGCVVLEMLTGKSP-------------RNNEEAIQIINDI 309

Query: 301 SSAGRDFVKRCLIRSPYKRRPIWLLIKHPFVCL 333
           S  GRDF+K+CLI SPYKRRP WLLIKHPFVC+
Sbjct: 310 SKEGRDFIKKCLITSPYKRRPSWLLIKHPFVCV 320

[Close]

BLAST of Lag0034312 vs. ExPASy Swiss-Prot
Match: Q9SND6 (Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK20 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 2.0e-42
Identity = 96/271 (35.42%), Postives = 149/271 (54.98%), Query Frame = 0


Query: 68  GSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYGDE 127
           G G        T +  + D P  I VKS     ++SL +EK VL  L  C  ++RCYG++
Sbjct: 10  GFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGED 69

Query: 128 ITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARY 187
            +T ++GE+++N+L EY S  SL  ++ K G  GLPE  VR++T  ++ GL H+H    +
Sbjct: 70  -STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG-F 129

Query: 188 IHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLC---DQEMSGSGPYISPE 247
            H DIK  NILL       + GS+  K+  FGLA ++  ++       E+ G+  Y++PE
Sbjct: 130 AHCDIKLANILLF------NDGSV--KIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 189

Query: 248 LARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYM---LDCYNNQLLPEISKEISS 307
              D      AD+WALGC V+EM +GK AWS +  + +M   +       LP+I + +S 
Sbjct: 190 CVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSE 249

Query: 308 AGRDFVKRCLIRSPYKRRPIWLLIKHPFVCL 333
            G+DF+ +C ++ P KR    +L+ H FV +
Sbjct: 250 EGKDFLSKCFVKDPAKRWTAEMLLNHSFVTI 270

[Close]

BLAST of Lag0034312 vs. ExPASy Swiss-Prot
Match: O80888 (Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK17 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.5e-29
Identity = 86/277 (31.05%), Postives = 140/277 (50.54%), Query Frame = 0


Query: 58  WIGDRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPC 117
           W   R+L +  +  V+ A        NSD    + VKS  +  S  LQ E ++LS L+  
Sbjct: 3   WTRGRILGRGSTATVYAAAGH-----NSD--EILAVKSSEVHRSEFLQREAKILSSLS-S 62

Query: 118 TNVVRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHG 177
             V+   G E     +G  +YN+L EY    +L D   K G   + E  V KYTRDI+ G
Sbjct: 63  PYVIGYRGSETKRESNGVVMYNLLMEYAPYGTLTDAAAKDG-GRVDETRVVKYTRDILKG 122

Query: 178 LYHMHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGS 237
           L ++H     +H D+K  N+++      ++KG   AK+  FG AK++ P  + +  + G+
Sbjct: 123 LEYIHSKG-IVHCDVKGSNVVI------SEKGE--AKIADFGCAKRVDP--VFESPVMGT 182

Query: 238 GPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAW----SFENANKYMLDCYNNQLLP 297
             +++PE+AR       +DIWA+GC ++EM+TG P W    S E+    +     +   P
Sbjct: 183 PAFMAPEVARGEKQGKESDIWAVGCTMIEMVTGSPPWTKADSREDPVSVLYRVGYSSETP 242

Query: 298 EISKEISSAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
           E+   ++   +DF+++CL R   +R     L+ HPF+
Sbjct: 243 ELPCLLAEEAKDFLEKCLKREANERWTATQLLNHPFL 259

[Close]

BLAST of Lag0034312 vs. ExPASy Swiss-Prot
Match: Q54R82 (Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=44689 GN=mkkA PE=1 SV=2)

HSP 1 Score: 123.2 bits (308), Expect = 5.4e-27
Identity = 93/310 (30.00%), Postives = 146/310 (47.10%), Query Frame = 0


Query: 31  DNNWNDSVKHYDGRFANLTVEE------SHGSGWIGDRLLRKTGSGLVFLAKNRTPTNPN 90
           +NN N+ ++H D  F +L + E      +H   W   ++L + G G V+L  N+      
Sbjct: 139 NNNNNNIIQHTD--FPSLIINEHEELISNHNIKWQKGQILGRGGYGSVYLGLNKDTGELF 198

Query: 91  SDLPTEIVVKSCFMELSS---SLQHEKEVLSDLAPCTNVVRCYGDEITTTKSGEKIYNVL 150
           +    EIV  +   +L +   S   E EV+  L    N+VR  G     T   +   +V 
Sbjct: 199 AVKQLEIVDINSDPKLKNMILSFSKEIEVMRSLRH-DNIVRYLG-----TSLDQSFLSVF 258

Query: 151 FEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNILLLP 210
            EY  G S+   + K G     E  ++ YT+ I+ GL  +H N+  IH DIK  NIL+  
Sbjct: 259 LEYIPGGSISSLLGKFG--AFSENVIKVYTKQILQGLSFLHANS-IIHRDIKGANILI-- 318

Query: 211 SACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPYISPELARDGYLDWPADIWALG 270
                 KG  I K++ FG +K  +  V   + M G+  +++PE+ +       +DIW+LG
Sbjct: 319 ----DTKG--IVKLSDFGCSKSFSGIVSQFKSMQGTPYWMAPEVIKQTGHGRSSDIWSLG 378

Query: 271 CVVLEMLTGKPAWS-FENANKYMLDCYNNQLLPEISKEISSAGRDFVKRCLIRSPYKRRP 330
           CV++EM T +P WS        M    ++  +P I   +S    DF+  C  R P +R  
Sbjct: 379 CVIVEMATAQPPWSNITELAAVMYHIASSNSIPNIPSHMSQEAFDFLNLCFKRDPKERPD 429

[Close]

BLAST of Lag0034312 vs. ExPASy Swiss-Prot
Match: Q9CAD5 (Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3702 GN=YDA PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 7.0e-27
Identity = 94/284 (33.10%), Postives = 136/284 (47.89%), Query Frame = 0


Query: 53  SHGSGWIGDRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSC-----FMELSSSLQHE 112
           S GS W   RLL     G V+L  N    + + ++     V  C       E +  L  E
Sbjct: 395 SPGSRWKKGRLLGMGSFGHVYLGFN----SESGEMCAMKEVTLCSDDPKSRESAQQLGQE 454

Query: 113 KEVLSDLAPCTNVVRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEV 172
             VLS L    N+V+ YG E       +K+Y +  EY SG S+   + + G  G  E  +
Sbjct: 455 ISVLSRLRH-QNIVQYYGSETV----DDKLY-IYLEYVSGGSIYKLLQEYGQFG--ENAI 514

Query: 173 RKYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPE 232
           R YT+ I+ GL ++H     +H DIK  NIL+ P            K+  FG+AK +T +
Sbjct: 515 RNYTQQILSGLAYLHAK-NTVHRDIKGANILVDPHG--------RVKVADFGMAKHITAQ 574

Query: 233 VLCDQEMSGSGPYISPELARDGY-LDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDC 292
                   GS  +++PE+ ++    +   DIW+LGC VLEM T KP WS       M   
Sbjct: 575 -SGPLSFKGSPYWMAPEVIKNSNGSNLAVDIWSLGCTVLEMATTKPPWSQYEGVPAMFKI 634

Query: 293 YNNQLLPEISKEISSAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
            N++ LP+I   +S  G+DFV++CL R+P  R     L+ H FV
Sbjct: 635 GNSKELPDIPDHLSEEGKDFVRKCLQRNPANRPTAAQLLDHAFV 656

[Close]

BLAST of Lag0034312 vs. ExPASy Swiss-Prot
Match: F4HRJ4 (Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702 GN=MAPKKK3 PE=1 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 2.5e-24
Identity = 76/234 (32.48%), Postives = 120/234 (51.28%), Query Frame = 0


Query: 99  ELSSSLQHEKEVLSDLAPCTNVVRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSG 158
           E    L  E  +L+ L    N+V+ YG E++     E+  +V  EY SG S+  H +   
Sbjct: 256 ECLKQLNQEINLLNQLCH-PNIVQYYGSELS-----EETLSVYLEYVSGGSI--HKLLKD 315

Query: 159 LNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSF 218
                E  ++ YTR I+ GL ++H     +H DIK  NIL+ P+      G +  K+  F
Sbjct: 316 YGSFTEPVIQNYTRQILAGLAYLH-GRNTVHRDIKGANILVDPN------GEI--KLADF 375

Query: 219 GLAKKLTPEVLCDQEMSGSGPYISPE--LARDGYLDWPADIWALGCVVLEMLTGKPAWSF 278
           G+AK +T          GS  +++PE  ++++GY     DIW+LGC +LEM T KP WS 
Sbjct: 376 GMAKHVT-AFSTMLSFKGSPYWMAPEVVMSQNGYTH-AVDIWSLGCTILEMATSKPPWSQ 435

Query: 279 ENANKYMLDCYNNQLLPEISKEISSAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
                 +    N++  PEI   +S+  ++F++ CL R+P  R     L++HPF+
Sbjct: 436 FEGVAAIFKIGNSKDTPEIPDHLSNDAKNFIRLCLQRNPTVRPTASQLLEHPFL 470

[Close]

BLAST of Lag0034312 vs. ExPASy TrEMBL
Match: A0A6J1HBP5 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=3662 GN=LOC111461386 PE=4 SV=1)

HSP 1 Score: 503.8 bits (1296), Expect = 5.4e-139
Identity = 241/330 (73.03%), Postives = 277/330 (83.94%), Query Frame = 0


Query: 1   MSEQSRNFVNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIG 60
           +++Q  N VNLH+FLKTKRGIVK AKK+    NWN++VKH  GRF N  +EE HGS WIG
Sbjct: 10  VNQQCHNIVNLHEFLKTKRGIVKCAKKSQSSTNWNNNVKHNYGRFGNFAIEECHGSFWIG 69

Query: 61  DRLLRKTGSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNV 120
           DRL+R++GSGLVFLAKN++ TN NS    E +VKSC ME SSSLQ+EKEVL +L PC+N+
Sbjct: 70  DRLIRRSGSGLVFLAKNKSSTNSNS----EFLVKSCLMESSSSLQYEKEVLCNLGPCSNL 129

Query: 121 VRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYH 180
           VRCYGDEIT +KSGE+IYNV+FEYC GSSLRDHIIK G NGLPE  VRKYTRDIV GL +
Sbjct: 130 VRCYGDEITMSKSGEQIYNVVFEYCCGSSLRDHIIKFGRNGLPEPIVRKYTRDIVRGLCY 189

Query: 181 MHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPY 240
           MHCNA YIHGDIKPRNILL PS      G L+AK+ SFGLA+KLTPEVLCD+E+SGSG Y
Sbjct: 190 MHCNAGYIHGDIKPRNILLSPSDHKG--GCLVAKLASFGLARKLTPEVLCDEEISGSGSY 249

Query: 241 ISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEIS 300
           I+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE+A  Y+++C  NQ +PEI  +IS
Sbjct: 250 IAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFESAYNYLMNC--NQEIPEIPSKIS 309

Query: 301 SAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
           SAGRDFVKRCLIRSPYKRRP WLL+KHPFV
Sbjct: 310 SAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 331

[Close]

BLAST of Lag0034312 vs. ExPASy TrEMBL
Match: A0A5D3D3K9 (Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1804G00170 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.5e-125
Identity = 223/326 (68.40%), Postives = 257/326 (78.83%), Query Frame = 0


Query: 9   VNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFA-NLTVEESHGSGWIGDRLLRKT 68
           VNLH+FLKTKR I+K AKK+ P NNWND++KH   +F  + T+EE HGS W G  L RKT
Sbjct: 14  VNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFHGSIWSGVHLFRKT 73

Query: 69  GSGLVFLA-KNRTP-TNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYG 128
            S L+FLA KNR P  N  S+LP E +VKS   E SSSL HEKEVLS+L P  N+V CYG
Sbjct: 74  ESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEKEVLSNLGPFMNLVDCYG 133

Query: 129 DEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNA 188
           DEIT +KSGEK+YNV FEYCSGSSLRDHI K G NGL E+EVRKYTRDIVHGLY+MHCN 
Sbjct: 134 DEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVRKYTRDIVHGLYYMHCNG 193

Query: 189 RYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPYISPEL 248
           RYIHGDIK RNILL            +AK+ SFGLA+KLT EV+C++E+SGSGPY SPEL
Sbjct: 194 RYIHGDIKSRNILLSHG---------MAKLASFGLARKLTAEVICEEEISGSGPYASPEL 253

Query: 249 ARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEISSAGRD 308
           AR+GYL WPADIWALGCVVLEM TGK AWSF +A++Y++DC NN+ +PEI K IS  GRD
Sbjct: 254 AREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDC-NNEKIPEIPKNISREGRD 313

Query: 309 FVKRCLIRSPYKRRPIWLLIKHPFVC 332
           F+K+CLIRSPYKRRP WLLIKHPFVC
Sbjct: 314 FIKKCLIRSPYKRRPSWLLIKHPFVC 329

[Close]

BLAST of Lag0034312 vs. ExPASy TrEMBL
Match: A0A1S3B829 (mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656 GN=LOC103487049 PE=4 SV=1)

HSP 1 Score: 456.8 bits (1174), Expect = 7.5e-125
Identity = 223/326 (68.40%), Postives = 257/326 (78.83%), Query Frame = 0


Query: 9   VNLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFA-NLTVEESHGSGWIGDRLLRKT 68
           VNLH+FLKTKR I+K AKK+ P NNWND++KH   +F  + T+EE HGS W G  L RKT
Sbjct: 14  VNLHEFLKTKRRILKIAKKSAPTNNWNDTIKHNYEKFGEDFTIEEFHGSIWSGVHLFRKT 73

Query: 69  GSGLVFLA-KNRTP-TNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYG 128
            S L+FLA KNR P  N  S+LP E +VKS   E SSSL HEKEVLS+L P  N+V CYG
Sbjct: 74  ESTLIFLAKKNRAPINNSTSNLPEEFLVKSSLTESSSSLHHEKEVLSNLGPFMNLVDCYG 133

Query: 129 DEITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNA 188
           DEIT +KSGEK+YNV FEYCSGSSLRDHI K G NGL E+EVRKYTRDIVHGLY+MHCN 
Sbjct: 134 DEITISKSGEKVYNVFFEYCSGSSLRDHIRKFGPNGLQEHEVRKYTRDIVHGLYYMHCNG 193

Query: 189 RYIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPYISPEL 248
           RYIHGDIK RNILL            +AK+ SFGLA+KLT EV+C++E+SGSGPY SPEL
Sbjct: 194 RYIHGDIKSRNILLSHG---------MAKLASFGLARKLTAEVICEEEISGSGPYASPEL 253

Query: 249 ARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEISSAGRD 308
           AR+GYL WPADIWALGCVVLEM TGK AWSF +A++Y++DC NN+ +PEI K IS  GRD
Sbjct: 254 AREGYLGWPADIWALGCVVLEMFTGKSAWSFGDAHRYLMDC-NNEKIPEIPKNISREGRD 313

Query: 309 FVKRCLIRSPYKRRPIWLLIKHPFVC 332
           F+K+CLIRSPYKRRP WLLIKHPFVC
Sbjct: 314 FIKKCLIRSPYKRRPSWLLIKHPFVC 329

[Close]

BLAST of Lag0034312 vs. ExPASy TrEMBL
Match: A0A0A0LHV1 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829110 PE=4 SV=1)

HSP 1 Score: 456.4 bits (1173), Expect = 9.8e-125
Identity = 223/325 (68.62%), Postives = 259/325 (79.69%), Query Frame = 0


Query: 10  NLHDFLKTKRGIVKYAKKTLPDNNWNDSVKHYDGRFA-NLTVEESHGSGWIGDRLLRKTG 69
           NLH+FLKTKR I+K AKK+ P NNWND++KH   +F  + T+EE HGS W G  L RKT 
Sbjct: 15  NLHEFLKTKRRILKIAKKSAPRNNWNDTIKHNYEKFGEDFTIEEFHGSIWTGSLLFRKTE 74

Query: 70  SGLVFLA-KNRTP-TNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYGD 129
           S LVFLA KNR P  N NS+LP E +VKS  ME SSSL+HEKEVLS+L P TN+V CYGD
Sbjct: 75  STLVFLAKKNRAPINNSNSNLPEEFLVKSSLMESSSSLRHEKEVLSNLGPFTNLVDCYGD 134

Query: 130 EITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNAR 189
           EIT TKSGE++YNV FEYCSGSSLR+HI+K G NGL + EVR+YTRDIV GLY+MHCN R
Sbjct: 135 EITVTKSGEEVYNVFFEYCSGSSLRNHILKFGPNGLQDDEVRRYTRDIVRGLYYMHCNGR 194

Query: 190 YIHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPYISPELA 249
           YIHGDIK RNILL            +AK+ SFGLA+KLT EV+C++E+SGSGPY SPELA
Sbjct: 195 YIHGDIKSRNILLSHG---------MAKLASFGLARKLTAEVICEEEISGSGPYASPELA 254

Query: 250 RDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYMLDCYNNQLLPEISKEISSAGRDF 309
           R+GYL WPADIWALGCVVLEM TGK AWSFE+A +Y++D  NN+ +PEI K IS  GRDF
Sbjct: 255 REGYLGWPADIWALGCVVLEMFTGKSAWSFEDAYRYLMDS-NNEKIPEIPKNISREGRDF 314

Query: 310 VKRCLIRSPYKRRPIWLLIKHPFVC 332
           +K+CLIRSPYKRRPIWLLIKHPFVC
Sbjct: 315 IKKCLIRSPYKRRPIWLLIKHPFVC 329

[Close]

BLAST of Lag0034312 vs. ExPASy TrEMBL
Match: A0A6J1JAH8 (mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=3661 GN=LOC111485031 PE=4 SV=1)

HSP 1 Score: 388.3 bits (996), Expect = 3.3e-104
Identity = 184/233 (78.97%), Postives = 204/233 (87.55%), Query Frame = 0


Query: 98  MELSSSLQHEKEVLSDLAPCTNVVRCYGDEITTTKSGEKIYNVLFEYCSGSSLRDHIIKS 157
           ME SSSLQ+EKEVL +L PC+N+VRCYGDEIT  KSGE+IYNV+FEYC GSSLRDHIIK 
Sbjct: 1   MESSSSLQYEKEVLCNLGPCSNLVRCYGDEITMNKSGEQIYNVVFEYCCGSSLRDHIIKF 60

Query: 158 GLNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNILLLPSACAADKGSLIAKMTS 217
           G NGLPE  VRKYTRDIVHGL +MHCNARYIHGDIKPRNILL PS      G L+AK+ S
Sbjct: 61  GRNGLPEPIVRKYTRDIVHGLCYMHCNARYIHGDIKPRNILLSPSDHKG--GCLVAKLAS 120

Query: 218 FGLAKKLTPEVLCDQEMSGSGPYISPELARDGYLDWPADIWALGCVVLEMLTGKPAWSFE 277
           FGLA+KLTPEVLCD+E+SGSG YI+PELA+DGYLDWPADIWALGCVVLEMLTGKPAWSFE
Sbjct: 121 FGLARKLTPEVLCDEEISGSGSYIAPELAKDGYLDWPADIWALGCVVLEMLTGKPAWSFE 180

Query: 278 NANKYMLDCYNNQLLPEISKEISSAGRDFVKRCLIRSPYKRRPIWLLIKHPFV 331
           +A  Y++DC  NQ +PEI  +ISSAGRDFVKRCLIRSPYKRRP WLL+KHPFV
Sbjct: 181 SAYNYLMDC--NQEIPEIPSKISSAGRDFVKRCLIRSPYKRRPTWLLVKHPFV 229

[Close]

BLAST of Lag0034312 vs. TAIR 10
Match: AT4G36950.1 (mitogen-activated protein kinase kinase kinase 21 )

HSP 1 Score: 185.7 bits (470), Expect = 6.2e-47
Identity = 102/259 (39.38%), Postives = 153/259 (59.07%), Query Frame = 0


Query: 79  TPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYGDEITTTKSGEKIY 138
           T  + +   P+ + VKS  +  S++L++E++VL DL  C+ +VRC+G E  T ++GE+IY
Sbjct: 22  TSGSSSKAFPSLMAVKSSGVVCSAALRNERDVLDDLGDCSEIVRCFG-EGRTVENGEEIY 81

Query: 139 NVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNIL 198
           N+  EY SG SL D I  SG   LPE+EVR++TR IV GL H+H N  + H DIK  N+L
Sbjct: 82  NLFLEYASGGSLADRIKSSG-EALPEFEVRRFTRSIVKGLCHIHGNG-FTHCDIKLENVL 141

Query: 199 LLPSACAADKGSLIAKMTSFGLAKKLTPEVLCDQEMSGSGPYISPELARDGYLDWPADIW 258
           +         G    K++ FGLAK+ + EV    E+ G+  Y++PE    G  + PADIW
Sbjct: 142 VF--------GDGDVKISDFGLAKRRSGEVCV--EIRGTPLYMAPESVNHGEFESPADIW 201

Query: 259 ALGCVVLEMLTGKPAWSFE-----NANKYMLDCYNNQLLPEISKEISSAGRDFVKRCLIR 318
           ALGC V+EM +GK AW  E     N    ++   +   +P I  E+S  G+DFV +C ++
Sbjct: 202 ALGCSVVEMSSGKTAWCLEDGVMNNVMSLLVRIGSGDEVPRIPVELSEEGKDFVSKCFVK 261

Query: 319 SPYKRRPIWLLIKHPFVCL 333
           +  +R    +L+ HPF+ +
Sbjct: 262 NAAERWTAEMLLDHPFLAV 267

[Close]

BLAST of Lag0034312 vs. TAIR 10
Match: AT3G50310.1 (mitogen-activated protein kinase kinase kinase 20 )

HSP 1 Score: 174.5 bits (441), Expect = 1.4e-43
Identity = 96/271 (35.42%), Postives = 149/271 (54.98%), Query Frame = 0


Query: 68  GSGLVFLAKNRTPTNPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYGDE 127
           G G        T +  + D P  I VKS     ++SL +EK VL  L  C  ++RCYG++
Sbjct: 10  GFGTFSTVSTATKSRNSGDFPALIAVKSTDAYGAASLSNEKSVLDSLGDCPEIIRCYGED 69

Query: 128 ITTTKSGEKIYNVLFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARY 187
            +T ++GE+++N+L EY S  SL  ++ K G  GLPE  VR++T  ++ GL H+H    +
Sbjct: 70  -STVENGEEMHNLLLEYASRGSLASYMKKLGGEGLPESTVRRHTGSVLRGLRHIHAKG-F 129

Query: 188 IHGDIKPRNILLLPSACAADKGSLIAKMTSFGLAKKLTPEVLC---DQEMSGSGPYISPE 247
            H DIK  NILL       + GS+  K+  FGLA ++  ++       E+ G+  Y++PE
Sbjct: 130 AHCDIKLANILLF------NDGSV--KIADFGLAMRVDGDLTALRKSVEIRGTPLYMAPE 189

Query: 248 LARDGYLDWPADIWALGCVVLEMLTGKPAWSFENANKYM---LDCYNNQLLPEISKEISS 307
              D      AD+WALGC V+EM +GK AWS +  + +M   +       LP+I + +S 
Sbjct: 190 CVNDNEYGSAADVWALGCAVVEMFSGKTAWSVKEGSHFMSLLIRIGVGDELPKIPEMLSE 249

Query: 308 AGRDFVKRCLIRSPYKRRPIWLLIKHPFVCL 333
            G+DF+ +C ++ P KR    +L+ H FV +
Sbjct: 250 EGKDFLSKCFVKDPAKRWTAEMLLNHSFVTI 270

[Close]

BLAST of Lag0034312 vs. TAIR 10
Match: AT3G46140.1 (Protein kinase superfamily protein )

HSP 1 Score: 167.5 bits (423), Expect = 1.8e-41
Identity = 114/319 (35.74%), Postives = 163/319 (51.10%), Query Frame = 0


Query: 26  KKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIGDRLLRKTGSGLVFLAKNRTPTNPNS 85
           K+  P ++ + SV+    +  +  V +S  S WI    L +   G V+LA     T+  +
Sbjct: 66  KRFTPKSSSSRSVEEITKQVFDGVVRKS--SSWIKSEFLGRGSYGSVYLA-----TSKKA 125

Query: 86  DLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYGDEIT---TTKSGEKI-YNVL 145
              T + +KS  +  +SSL  E+ +L+ L+    +VRCYG EI    T   GE+  YN++
Sbjct: 126 KTKTTMAIKSAEISRASSLMDEERILTRLS-SPFIVRCYGHEIAREETLFGGERTNYNLI 185

Query: 146 FEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNILLLP 205
            EYCSG SL D ++   L GL E +V+   RDI++GL  +H  A  IH DIKP NI L P
Sbjct: 186 LEYCSGKSLFD-LVNDNLGGLSEKDVKLLARDILYGLDCIH-RANIIHCDIKPENIFLTP 245

Query: 206 SACAADKGSLIAKMTSFGLA--KKLTPEVLCDQEMSGSGPYISPELARDGYLDWPADIWA 265
                     +AK+  FGLA  K  +          G+  Y+SPEL R G +D+  D WA
Sbjct: 246 VENRIRPSGYVAKIGDFGLALEKGSSEYEKASGHRRGTTRYMSPELIRHGIVDYAVDTWA 305

Query: 266 LGCVVLEMLTGKPAWSFENANKYMLD--------CYNNQLLPEISKEISSAGRDFVKRCL 325
            GC VLEMLTG+  W  E+++   +D        CY    +P I   +S   + F+ RCL
Sbjct: 306 FGCTVLEMLTGQQVWG-EHSDLGSVDWDILIGQSCY----IPYIPDWLSEEAQHFLSRCL 365

Query: 326 IRSPYKRRPIWLLIKHPFV 331
            R P  R  I  L+ HPF+
Sbjct: 366 KRDPASRWGIGALLNHPFL 369

[Close]

BLAST of Lag0034312 vs. TAIR 10
Match: AT5G67080.1 (mitogen-activated protein kinase kinase kinase 19 )

HSP 1 Score: 166.8 bits (421), Expect = 3.0e-41
Identity = 91/255 (35.69%), Postives = 142/255 (55.69%), Query Frame = 0


Query: 82  NPNSDLPTEIVVKSCFMELSSSLQHEKEVLSDLA-PCTNVVRCYGDEITTTKSGEKIYNV 141
           N + + P  + VKS     ++SL +EK VL +L   C  +VRC+G++  T ++GE+++N+
Sbjct: 25  NDSGEFPPLMAVKSADSYGAASLANEKSVLDNLGDDCNEIVRCFGED-RTVENGEEMHNL 84

Query: 142 LFEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNILLL 201
             EY S  SL  ++ K    G+PE  VR++T  ++ GL H+H N  + H D+K  NILL 
Sbjct: 85  FLEYASRGSLESYLKKLAGEGVPESTVRRHTGSVLRGLRHIHANG-FAHCDLKLGNILLF 144

Query: 202 PSACAADKGSLIAKMTSFGLAKKLTPEVLCDQ--EMSGSGPYISPELARDGYLDWPADIW 261
                   G    K+  FGLAK++      +   ++ G+  Y++PE   D       D+W
Sbjct: 145 --------GDGAVKIADFGLAKRIGDLTALNYGVQIRGTPLYMAPESVNDNEYGSEGDVW 204

Query: 262 ALGCVVLEMLTGKPAWSFENANKYM---LDCYNNQLLPEISKEISSAGRDFVKRCLIRSP 321
           ALGCVV+EM +GK AWS +  + +M   L       +P I +E+S  GRDF+ +C ++ P
Sbjct: 205 ALGCVVVEMFSGKTAWSLKEGSNFMSLLLRIGVGDEVPMIPEELSEQGRDFLSKCFVKDP 264

Query: 322 YKRRPIWLLIKHPFV 331
            KR    +L+ HPFV
Sbjct: 265 KKRWTAEMLLNHPFV 269

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BLAST of Lag0034312 vs. TAIR 10
Match: AT3G45790.1 (Protein kinase superfamily protein )

HSP 1 Score: 161.4 bits (407), Expect = 1.3e-39
Identity = 110/304 (36.18%), Postives = 158/304 (51.97%), Query Frame = 0


Query: 26  KKTLPDNNWNDSVKHYDGRFANLTVEESHGSGWIGDRLLRKTGSGLVFLAKNRTPTNPNS 85
           K+  P ++ + SV+    +  +  V +S  S WI    L +   G V+LA     T+  +
Sbjct: 66  KRFTPKSSSSLSVEEITKQVFDGVVRKS--SSWIKSEFLGRGSYGSVYLA-----TSKKA 125

Query: 86  DLPTEIVVKSCFMELSSSLQHEKEVLSDLAPCTNVVRCYGDEIT---TTKSGEKI-YNVL 145
              T + +KS  +  +SSL  E+ +L+ L+    +VRCYG EI    T   GE+  YN++
Sbjct: 126 KTKTTMAIKSAEISRASSLMDEERILTRLS-SPFIVRCYGHEIAREETLFGGERTNYNLI 185

Query: 146 FEYCSGSSLRDHIIKSGLNGLPEYEVRKYTRDIVHGLYHMHCNARYIHGDIKPRNILLLP 205
            EYCSG SL D ++ S L GL E +V+   RDI++GL ++H  A  IH DIKP NILL P
Sbjct: 186 LEYCSGKSLFD-LVNSNLGGLSEKDVKLLARDILYGLDYIH-RANIIHCDIKPENILLAP 245

Query: 206 SACAADKGSLIAKMTSFGLA--KKLTPEVLCDQEMSGSGPYISPELARDGYLDWPADIWA 265
                     +AK+  FGLA  K  +          G+  Y+SPEL R G +D+  D WA
Sbjct: 246 VENRIRPNGYVAKIGDFGLALEKGSSEYEKASGHRRGTTRYMSPELIRHGIVDYAVDTWA 305

Query: 266 LGCVVLEMLTGKPAWSFENANKYMLD--------CYNNQLLPEISKEISSAGRDFVKRCL 316
            GC VLEMLTG+  W  E+++   +D        CY    +P I   +S   + F+ RCL
Sbjct: 306 FGCTVLEMLTGQQVWG-EHSDLGSVDWDILIGQSCY----IPYIPDWLSEEAQHFLSRCL 354

The following BLAST results are available for this feature:

BLAST of Lag0034312 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023516554.1	4.5e-140	73.64	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita pepo subsp. pe...	[more]
KAG7023508.1	8.5e-139	73.33	Mitogen-activated protein kinase kinase kinase 17, partial [Cucurbita argyrosper...	[more]
XP_022960664.1	1.1e-138	73.03	mitogen-activated protein kinase kinase kinase 17-like [Cucurbita moschata]	[more]
KAG6589837.1	1.9e-138	73.03	Mitogen-activated protein kinase kinase kinase 20, partial [Cucurbita argyrosper...	[more]
XP_038880300.1	2.8e-126	66.97	mitogen-activated protein kinase kinase kinase 17-like [Benincasa hispida]	[more]

BLAST of Lag0034312 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SND6	2.0e-42	35.42	Mitogen-activated protein kinase kinase kinase 20 OS=Arabidopsis thaliana OX=370...	[more]
O80888	7.5e-29	31.05	Mitogen-activated protein kinase kinase kinase 17 OS=Arabidopsis thaliana OX=370...	[more]
Q54R82	5.4e-27	30.00	Mitogen-activated protein kinase kinase kinase A OS=Dictyostelium discoideum OX=...	[more]
Q9CAD5	7.0e-27	33.10	Mitogen-activated protein kinase kinase kinase YODA OS=Arabidopsis thaliana OX=3...	[more]
F4HRJ4	2.5e-24	32.48	Mitogen-activated protein kinase kinase kinase 3 OS=Arabidopsis thaliana OX=3702...	[more]

BLAST of Lag0034312 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBP5	5.4e-139	73.03	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita moschata OX=...	[more]
A0A5D3D3K9	7.5e-125	68.40	Mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo var. ma...	[more]
A0A1S3B829	7.5e-125	68.40	mitogen-activated protein kinase kinase kinase YODA-like OS=Cucumis melo OX=3656...	[more]
A0A0A0LHV1	9.8e-125	68.62	Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G829...	[more]
A0A6J1JAH8	3.3e-104	78.97	mitogen-activated protein kinase kinase kinase 17-like OS=Cucurbita maxima OX=36...	[more]

BLAST of Lag0034312 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36950.1	6.2e-47	39.38	mitogen-activated protein kinase kinase kinase 21	[more]
AT3G50310.1	1.4e-43	35.42	mitogen-activated protein kinase kinase kinase 20	[more]
AT3G46140.1	1.8e-41	35.74	Protein kinase superfamily protein	[more]
AT5G67080.1	3.0e-41	35.69	mitogen-activated protein kinase kinase kinase 19	[more]
AT3G45790.1	1.3e-39	36.18	Protein kinase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000719	Protein kinase domain	SMART	SM00220	serkin_6	coord: 58..330 e-value: 3.5E-44 score: 162.8
IPR000719	Protein kinase domain	PFAM	PF00069	Pkinase	coord: 102..330 e-value: 6.6E-36 score: 124.0
IPR000719	Protein kinase domain	PROSITE	PS50011	PROTEIN_KINASE_DOM	coord: 58..330 score: 33.46014
None	No IPR available	GENE3D	1.10.510.10	Transferase(Phosphotransferase) domain 1	coord: 59..332 e-value: 5.8E-46 score: 159.1
None	No IPR available	PANTHER	PTHR48011	CCR4-NOT TRANSCRIPTIONAL COMPLEX SUBUNIT CAF120-RELATED	coord: 45..330
None	No IPR available	PANTHER	PTHR48011:SF55	SUBFAMILY NOT NAMED	coord: 45..330
IPR008271	Serine/threonine-protein kinase, active site	PROSITE	PS00108	PROTEIN_KINASE_ST	coord: 187..199
IPR011009	Protein kinase-like domain superfamily	SUPERFAMILY	56112	Protein kinase-like (PK-like)	coord: 56..330

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0034312.1	Lag0034312.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006468	protein phosphorylation
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0004672	protein kinase activity