Lag0032774 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032774
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Protein DETOXIFICATION
Location	chr11: 37396122 .. 37397702 (-)
RNA-Seq Expression	Lag0032774
Synteny	Lag0032774

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTGACTCTGTTGCTTCTTCATCTTCTGTACTTTCCGACCAACCAAACAGTCCGACAAGTACTGATTCACTCAGAGCTCCTCTGATCTCCGAAACTCCAACAACTCCAACAACAACAGACAAATTTCCATTTCTCAGTCTTGTCCTCACAGAGTCCAAATCCATAGCCGAAATCGCATTTCCGATGACTCTTGTCGGCTTTTTGATGTACTCTCGTTCCATGATTTCCATGTTGTTCCTCGGCCGGTTGGGCGGTTTGTCCTTGGCCGGTGGTGCGCTTGCAATTGGGTTTGCTAATATCACTGGCTACTCTGTTCTCTCTGGTTTGGCCATTGGTATGGAGCCCATTTGCGGCCAAGCTTTTGGGGCTAAAAGATTCAAGCTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTTCTCCTTGCTTCCATACCCATTTCGTTTTTGTGGTTCAATATGAAGAAAATCCTTCTCTTGTGCGGCCAAAACGATGATATTGCCAATGAAGCTCATTCCTTCATCCTCTGTTCTCTCCCCGATTTAATTGCTCTGTCTTTTCTTCACCCTTTGCGAATTTACCTTCGTAGTCAATCCATTAACCTCCCTCTCACTTACTGCGCTGGATTGGCCGTTCTTTTCCACATCCCCATTAATTACCTTCTCGTCTCTGTTCTCGAAATGGGAATTTACGGCGTCGCTTTGGGAGCTGTGTGGGCGAATTTCAACCTCGTCGGATCACTGATTGTTTTCATCATCATCTCCGGCGTGTACGAGAAAACCTGGCCGGGAATCTCATCGGAGTGTTTAAAGGGGTGGAGATCGCTTCTCGGATTGGCGATTCCGAGCTGTGTTTCCGTCTGTTTGGAGTGGTGGTGGTACGAAATCATGATTTTGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCAATGGGGATTTTGATACAGACGACAGCTTTAATCTACATTTTCCCATCGTCACTAAGCTTCGGAGTATCAACAAGAGTGGGAAACGAACTGGGAGCAAATCGTCCAAACAGAGCGAAATTAACCGCCATTGTTGGGCTCTGTTCCAGCTTCTTCCTCGGCCTCGCGGCATTGGTGTTCGCTTTCAGCGTCCGCAGAGTCTGGGCCACAATGTTCACAGAGGACACACAGATCATCGAGCTGACATCTCTAGTTCTCCCAATAATCGGGCTCTGCGAGCTGGGAAACTGCCCACAGACGGCGAGCTGCGGAGTTTTGAGAGGCACGGCGAGGCCAAAACTGGGGGCGAATATAAATTTGGGATGTTTTTACATTGTGGGACTGCCGGTGGCGATATGGCTGAGCTTCTACGGCGGGTGGGACTTCAGAGGGCTGTGGATTGGGCTGCTGGCGGCGCAGGGGTCGTGTGCCATGGCGATGCTGATGACAGTGGCTTGGACCAATTGGGAAGAAGAGGTCGAGAGGGCTAAGGAGTTGACCAGAAGTGGCTCGGAGGAAGAGGTTGAAGATGGCGATGGTGTTGAAGAAAACGAGACATTGGAGGATTTGAATTCAATGAATCGAAGTGTTTTGGAAGTGTGA

mRNA sequence

ATGTCTGACTCTGTTGCTTCTTCATCTTCTGTACTTTCCGACCAACCAAACAGTCCGACAAGTACTGATTCACTCAGAGCTCCTCTGATCTCCGAAACTCCAACAACTCCAACAACAACAGACAAATTTCCATTTCTCAGTCTTGTCCTCACAGAGTCCAAATCCATAGCCGAAATCGCATTTCCGATGACTCTTGTCGGCTTTTTGATGTACTCTCGTTCCATGATTTCCATGTTGTTCCTCGGCCGGTTGGGCGGTTTGTCCTTGGCCGGTGGTGCGCTTGCAATTGGGTTTGCTAATATCACTGGCTACTCTGTTCTCTCTGGTTTGGCCATTGGTATGGAGCCCATTTGCGGCCAAGCTTTTGGGGCTAAAAGATTCAAGCTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTTCTCCTTGCTTCCATACCCATTTCGTTTTTGTGGTTCAATATGAAGAAAATCCTTCTCTTGTGCGGCCAAAACGATGATATTGCCAATGAAGCTCATTCCTTCATCCTCTGTTCTCTCCCCGATTTAATTGCTCTGTCTTTTCTTCACCCTTTGCGAATTTACCTTCGTAGTCAATCCATTAACCTCCCTCTCACTTACTGCGCTGGATTGGCCGTTCTTTTCCACATCCCCATTAATTACCTTCTCGTCTCTGTTCTCGAAATGGGAATTTACGGCGTCGCTTTGGGAGCTGTGTGGGCGAATTTCAACCTCGTCGGATCACTGATTGTTTTCATCATCATCTCCGGCGTGTACGAGAAAACCTGGCCGGGAATCTCATCGGAGTGTTTAAAGGGGTGGAGATCGCTTCTCGGATTGGCGATTCCGAGCTGTGTTTCCGTCTGTTTGGAGTGGTGGTGGTACGAAATCATGATTTTGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCAATGGGGATTTTGATACAGACGACAGCTTTAATCTACATTTTCCCATCGTCACTAAGCTTCGGAGTATCAACAAGAGTGGGAAACGAACTGGGAGCAAATCGTCCAAACAGAGCGAAATTAACCGCCATTGTTGGGCTCTGTTCCAGCTTCTTCCTCGGCCTCGCGGCATTGGTGTTCGCTTTCAGCGTCCGCAGAGTCTGGGCCACAATGTTCACAGAGGACACACAGATCATCGAGCTGACATCTCTAGTTCTCCCAATAATCGGGCTCTGCGAGCTGGGAAACTGCCCACAGACGGCGAGCTGCGGAGTTTTGAGAGGCACGGCGAGGCCAAAACTGGGGGCGAATATAAATTTGGGATGTTTTTACATTGTGGGACTGCCGGTGGCGATATGGCTGAGCTTCTACGGCGGGTGGGACTTCAGAGGGCTGTGGATTGGGCTGCTGGCGGCGCAGGGGTCGTGTGCCATGGCGATGCTGATGACAGTGGCTTGGACCAATTGGGAAGAAGAGGTCGAGAGGGCTAAGGAGTTGACCAGAAGTGGCTCGGAGGAAGAGGTTGAAGATGGCGATGGTGTTGAAGAAAACGAGACATTGGAGGATTTGAATTCAATGAATCGAAGTGTTTTGGAAGTGTGA

Coding sequence (CDS)

ATGTCTGACTCTGTTGCTTCTTCATCTTCTGTACTTTCCGACCAACCAAACAGTCCGACAAGTACTGATTCACTCAGAGCTCCTCTGATCTCCGAAACTCCAACAACTCCAACAACAACAGACAAATTTCCATTTCTCAGTCTTGTCCTCACAGAGTCCAAATCCATAGCCGAAATCGCATTTCCGATGACTCTTGTCGGCTTTTTGATGTACTCTCGTTCCATGATTTCCATGTTGTTCCTCGGCCGGTTGGGCGGTTTGTCCTTGGCCGGTGGTGCGCTTGCAATTGGGTTTGCTAATATCACTGGCTACTCTGTTCTCTCTGGTTTGGCCATTGGTATGGAGCCCATTTGCGGCCAAGCTTTTGGGGCTAAAAGATTCAAGCTTTTGGGCCTTGCCCTTCAAAGAACAGTCATTCTTCTTCTCCTTGCTTCCATACCCATTTCGTTTTTGTGGTTCAATATGAAGAAAATCCTTCTCTTGTGCGGCCAAAACGATGATATTGCCAATGAAGCTCATTCCTTCATCCTCTGTTCTCTCCCCGATTTAATTGCTCTGTCTTTTCTTCACCCTTTGCGAATTTACCTTCGTAGTCAATCCATTAACCTCCCTCTCACTTACTGCGCTGGATTGGCCGTTCTTTTCCACATCCCCATTAATTACCTTCTCGTCTCTGTTCTCGAAATGGGAATTTACGGCGTCGCTTTGGGAGCTGTGTGGGCGAATTTCAACCTCGTCGGATCACTGATTGTTTTCATCATCATCTCCGGCGTGTACGAGAAAACCTGGCCGGGAATCTCATCGGAGTGTTTAAAGGGGTGGAGATCGCTTCTCGGATTGGCGATTCCGAGCTGTGTTTCCGTCTGTTTGGAGTGGTGGTGGTACGAAATCATGATTTTGCTAAGTGGGTTCATGATGAATCCTCAATCAACAGTGGCTTCAATGGGGATTTTGATACAGACGACAGCTTTAATCTACATTTTCCCATCGTCACTAAGCTTCGGAGTATCAACAAGAGTGGGAAACGAACTGGGAGCAAATCGTCCAAACAGAGCGAAATTAACCGCCATTGTTGGGCTCTGTTCCAGCTTCTTCCTCGGCCTCGCGGCATTGGTGTTCGCTTTCAGCGTCCGCAGAGTCTGGGCCACAATGTTCACAGAGGACACACAGATCATCGAGCTGACATCTCTAGTTCTCCCAATAATCGGGCTCTGCGAGCTGGGAAACTGCCCACAGACGGCGAGCTGCGGAGTTTTGAGAGGCACGGCGAGGCCAAAACTGGGGGCGAATATAAATTTGGGATGTTTTTACATTGTGGGACTGCCGGTGGCGATATGGCTGAGCTTCTACGGCGGGTGGGACTTCAGAGGGCTGTGGATTGGGCTGCTGGCGGCGCAGGGGTCGTGTGCCATGGCGATGCTGATGACAGTGGCTTGGACCAATTGGGAAGAAGAGGTCGAGAGGGCTAAGGAGTTGACCAGAAGTGGCTCGGAGGAAGAGGTTGAAGATGGCGATGGTGTTGAAGAAAACGAGACATTGGAGGATTTGAATTCAATGAATCGAAGTGTTTTGGAAGTGTGA

Protein sequence

MSDSVASSSSVLSDQPNSPTSTDSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEEEVERAKELTRSGSEEEVEDGDGVEENETLEDLNSMNRSVLEV

Homology

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BLAST of Lag0032774 vs. NCBI nr
Match: XP_022970176.1 (protein DETOXIFICATION 49-like [Cucurbita maxima])

HSP 1 Score: 830.1 bits (2143), Expect = 1.1e-236
Identity = 433/525 (82.48%), Postives = 472/525 (89.90%), Query Frame = 0


Query: 1   MSDSVASSSSVLSDQPNSPTSTDSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIA 60
           MS S + S SVLSDQP  PT+T SL+APLISE       T++FP L LVLTE K IA+IA
Sbjct: 1   MSGSGSGSGSVLSDQPKIPTNTTSLKAPLISE-------TERFPNLCLVLTELKCIADIA 60

Query: 61  FPMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQ 120
            PM LVGFLMYSRSMISMLFLGRLGGLSLAGG+LAIGFANITGYSVLSGLA+GMEPICGQ
Sbjct: 61  LPMVLVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQ 120

Query: 121 AFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSL 180
           AFGA++FKLLGLALQRTVILLL +SIPISFLWFNMKKILLLCGQNDDIA+EAHS+ILCS+
Sbjct: 121 AFGAQKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSV 180

Query: 181 PDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVW 240
           PDLIALSFLHPLRIYLRSQSINLPLTYCAGLA++FHIPINYLLVSV + GIYGVALGAVW
Sbjct: 181 PDLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDWGIYGVALGAVW 240

Query: 241 ANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMIL 300
            NFNLVG LI+FII+SGVY+KTWPG+SSECLK W+SLL LAIPSC+SVCLEWWWYEIMIL
Sbjct: 241 TNFNLVGLLILFIIVSGVYKKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMIL 300

Query: 301 LSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGL 360
           LSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPN+AKL AIVGL
Sbjct: 301 LSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGL 360

Query: 361 CSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLR 420
           C+SFFLGL+AL FAF+VR+VWA MFT DT IIELT LVLPIIGLCELGNCPQT SCGVLR
Sbjct: 361 CTSFFLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLR 420

Query: 421 GTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWT 480
           GTARPKLGANINLGCFY+VG+P+AIWLSFYGGWDF+GLWIGLLAAQGSCA+AMLM +  T
Sbjct: 421 GTARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQGSCAIAMLMALVRT 480

Query: 481 NWEEEVERAKELTRSGSEEEVEDGDGVEENETLEDLNSMNRSVLE 526
           NWEEE ERAKELT SGS+         + +E  E LNS++RS LE
Sbjct: 481 NWEEEAERAKELTGSGSD---------DNDEKTELLNSIHRSDLE 509

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BLAST of Lag0032774 vs. NCBI nr
Match: XP_022958539.1 (protein DETOXIFICATION 49-like [Cucurbita moschata])

HSP 1 Score: 820.8 bits (2119), Expect = 6.5e-234
Identity = 429/529 (81.10%), Postives = 470/529 (88.85%), Query Frame = 0


Query: 6   ASSSSVLSDQPNSPTSTDSLRAPLISETPTTPTT-TDKFPFLSLVLTESKSIAEIAFPMT 65
           ASS  +LSDQPNSPT+  SL APLISE PT  TT  DK+PFL L+L+E KSIA +A PM 
Sbjct: 3   ASSVPLLSDQPNSPTTNHSLTAPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPMI 62

Query: 66  LVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGA 125
            VGF MYSRSMISMLFLG+LG LSLAGG+LAIGFANITGYSVLSGLA GMEPICGQAFGA
Sbjct: 63  FVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGA 122

Query: 126 KRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLI 185
           KRFKLLGLALQRTVILLLL+SIPISFLW NMKKIL+ CGQ DDIANEAHSFILCS+PDLI
Sbjct: 123 KRFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLI 182

Query: 186 ALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFN 245
           ALSFLHPLRIYLR QSINLPLTYC GL+VLFH+PINYLLVSVL++GI+GVALGAVW NFN
Sbjct: 183 ALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHVPINYLLVSVLDLGIHGVALGAVWTNFN 242

Query: 246 LVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGF 305
           LVGS+I+FI++ GVY+ +WPG SSECLK W+SLL LAIPSCVSVCLEWWWYEIMI+LSGF
Sbjct: 243 LVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGF 302

Query: 306 MMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSF 365
           MMNPQSTVASMGILIQTTALIYIFPSSLS GVSTRVGNELGAN+PNRAKL AIVGLC SF
Sbjct: 303 MMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSF 362

Query: 366 FLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTAR 425
           F GL+ALVFAF VR+VWATMFT DT+IIELTSLVLPIIGLCELGNCPQT +CGVLRGTAR
Sbjct: 363 FFGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTAR 422

Query: 426 PKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEE 485
           P LGANINL CFYIVG+P+AIWLSFYGGWDF+GLWIGLLAAQ SCAMAMLM +A TNWEE
Sbjct: 423 PTLGANINLVCFYIVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEE 482

Query: 486 EVERAKELTRSGSEEEVEDGDGVEENETLED-------LNSMNRSVLEV 527
           + ERA+ELTR+G   E+E+G+ VE+NET E+       LNSM    LEV
Sbjct: 483 QAERAQELTRTG-PLEIENGEDVEQNETFENDDDRNGRLNSMVERDLEV 530

[Close]

BLAST of Lag0032774 vs. NCBI nr
Match: KAG7016948.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 817.4 bits (2110), Expect = 7.2e-233
Identity = 420/499 (84.17%), Postives = 459/499 (91.98%), Query Frame = 0


Query: 5   VASSSSVLSDQPNSPT-STDSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIAFPM 64
           ++ S SVLSDQP  PT +T SL APLISE       T++FP L +VLTE K IA+IA PM
Sbjct: 1   MSGSGSVLSDQPKIPTNTTTSLNAPLISE-------TERFPNLCVVLTELKCIADIALPM 60

Query: 65  TLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFG 124
            LVGFLMYSRSMISMLFLGRLGGLSLAGG+LAIGFANITGYSVLSGLA+GMEPICGQAFG
Sbjct: 61  ILVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFG 120

Query: 125 AKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDL 184
           AK+FKLLGLALQRTVILLL +SIPISFLWFNMKKILLLCGQNDDIA+EAHS+ILCS+PDL
Sbjct: 121 AKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDL 180

Query: 185 IALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANF 244
           IALSFLHPLRIYLRSQSINLPLTYCAGLA++FHIPINYLLVSV ++GIYGVALGAVW NF
Sbjct: 181 IALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNF 240

Query: 245 NLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSG 304
           NLVG LI+FI++SGVYEKTWPG+SSECLK W+SLL LAIPSC+SVCLEWWWYEIMILLSG
Sbjct: 241 NLVGLLILFIVVSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSG 300

Query: 305 FMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSS 364
           FMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPN+AKL AIVGLC+S
Sbjct: 301 FMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTS 360

Query: 365 FFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTA 424
           FFLGL+AL FAF+VR+VWA MFT DT IIELT LVLPIIGLCELGNCPQT SCGVLRGTA
Sbjct: 361 FFLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTA 420

Query: 425 RPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWE 484
           RPKLGANINLGCFY+VG+P+AIWLSFYGGWDF+GLWIGLLAAQ SCA+AMLM +  TNWE
Sbjct: 421 RPKLGANINLGCFYLVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWE 480

Query: 485 EEVERAKELTRSGSEEEVE 503
           E+ ERAKELT SGS+++ E
Sbjct: 481 EQAERAKELTGSGSDDDDE 492

[Close]

BLAST of Lag0032774 vs. NCBI nr
Match: KAG6606121.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 817.0 bits (2109), Expect = 9.4e-233
Identity = 428/529 (80.91%), Postives = 469/529 (88.66%), Query Frame = 0


Query: 6   ASSSSVLSDQPNSPTSTDSLRAPLISETPTTPTT-TDKFPFLSLVLTESKSIAEIAFPMT 65
           ASS  +LSDQPNSPT+  SL  PLISE PT  TT  DK+PFL L+L+E KSIA +A PM 
Sbjct: 3   ASSVPLLSDQPNSPTTNHSLTPPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPMI 62

Query: 66  LVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGA 125
            VGF MYSRSMISMLFLG+LG LSLAGG+LAIGFANITGYSVLSGLA GMEPICGQAFGA
Sbjct: 63  FVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGA 122

Query: 126 KRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLI 185
           KRFKLLGLALQRTVILLLL+SIPISFLW NMKKIL+ CGQ DDIANEAHSFILCS+PDLI
Sbjct: 123 KRFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLI 182

Query: 186 ALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFN 245
           ALSFLHPLRIYLR QSINLPLTYC GL+VLFHIPINYLLVSVL++GI+GVALGAVW NFN
Sbjct: 183 ALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHIPINYLLVSVLDLGIHGVALGAVWTNFN 242

Query: 246 LVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGF 305
           LVGS+I+FI++ GVY+ +WPG SS CLK W+SLL LAIPSCVSVCLEWWWYEIMI+LSGF
Sbjct: 243 LVGSVIIFILVFGVYKNSWPGFSSACLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGF 302

Query: 306 MMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSF 365
           MMNPQSTVASMGILIQTTALIYIFPSSLS GVSTRVGNELGAN+PNRAKL AIVGLC SF
Sbjct: 303 MMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSF 362

Query: 366 FLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTAR 425
           F GL+ALVFAF VR+VWATMFT DT+IIELTSLVLPIIGLCELGNCPQT +CGVLRGTAR
Sbjct: 363 FSGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTAR 422

Query: 426 PKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEE 485
           P LGANINL CFY+VG+P+AIWLSFYGGWDF+GLWIGLLAAQ SCAMAMLM +A TNWEE
Sbjct: 423 PTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEE 482

Query: 486 EVERAKELTRSGSEEEVEDGDGVEENETLED-------LNSMNRSVLEV 527
           + ERA+ELTR+G   E+E+G+ VE+NET E+       LNSM  S LEV
Sbjct: 483 QAERAQELTRTG-PLEIENGEDVEQNETFENDDDRNGRLNSMVESDLEV 530

[Close]

BLAST of Lag0032774 vs. NCBI nr
Match: KAG6579480.1 (Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 815.5 bits (2105), Expect = 2.7e-232
Identity = 419/499 (83.97%), Postives = 459/499 (91.98%), Query Frame = 0


Query: 5   VASSSSVLSDQPNSPT-STDSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIAFPM 64
           ++ S SVLSDQP  PT +T SL APLISE       T++FP L +VLTE K IA+IA PM
Sbjct: 1   MSGSGSVLSDQPKIPTNTTTSLNAPLISE-------TERFPNLCVVLTELKCIADIALPM 60

Query: 65  TLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFG 124
            LVGFLMYSRSMISMLFLGRLGGLSLAGG+LAIGFANITGYSVLSGLA+GMEPICGQAFG
Sbjct: 61  ILVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFG 120

Query: 125 AKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDL 184
           AK+FKLLGLALQRTVILLL +SIPISFLWFNMKKILLLCGQNDDIA+EAHS+ILCS+PDL
Sbjct: 121 AKKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDL 180

Query: 185 IALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANF 244
           IALSFLHPLRIYLRSQSINLPLTYCAGLA++FHIPINYLLVSV ++GIYGVALGAVW NF
Sbjct: 181 IALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNF 240

Query: 245 NLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSG 304
           NLVG LI+FI++SGVYEKTWPG+SSECLK W+SLL LAIPSC+SVCLEWWWYEIMILLSG
Sbjct: 241 NLVGLLILFIVVSGVYEKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSG 300

Query: 305 FMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSS 364
           FMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRP++AKL AIVGLC+S
Sbjct: 301 FMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPSKAKLAAIVGLCTS 360

Query: 365 FFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTA 424
           FFLGL+AL FAF+VR+VWA MFT DT IIELT LVLPIIGLCELGNCPQT SCGVLRGTA
Sbjct: 361 FFLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTA 420

Query: 425 RPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWE 484
           RPKLGANINLGCFY+VG+P+AIWLSFYGGWDF+GLWIGLLAAQ SCA+AMLM +  TNWE
Sbjct: 421 RPKLGANINLGCFYLVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWE 480

Query: 485 EEVERAKELTRSGSEEEVE 503
           E+ ERAKELT SGS+++ E
Sbjct: 481 EQAERAKELTGSGSDDDDE 492

[Close]

BLAST of Lag0032774 vs. ExPASy Swiss-Prot
Match: O82752 (Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 1.9e-180
Identity = 323/461 (70.07%), Postives = 385/461 (83.51%), Query Frame = 0


Query: 46  LSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLGRLGGLS-LAGGALAIGFANITGY 105
           LS  + E+KSIA+I+ P+ L G L+YSRSMISMLFLGRL  LS L+GG+LA+GFANITGY
Sbjct: 26  LSSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGY 85

Query: 106 SVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQ 165
           S+LSGL+IGMEPIC QAFGAKRFKLLGLALQRT +LLLL S+PIS LW N+KKILL  GQ
Sbjct: 86  SLLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQ 145

Query: 166 NDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLV 225
           +++I+N+A  FIL SLPDLI  SFLHP+RIYLRSQSI LPLTY A  AVL HIPINYLLV
Sbjct: 146 DEEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLV 205

Query: 226 SVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPS 285
           S L +G+ GVALGA+W N NL+G LI++I+ SGVY+KTW G S +C KGWRSL+ LAIPS
Sbjct: 206 SSLGLGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPS 265

Query: 286 CVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNEL 345
           CVSVCLEWWWYEIMILL G ++NPQ+TVASMGILIQTTALIYIFPSSLS  VSTRVGNEL
Sbjct: 266 CVSVCLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNEL 325

Query: 346 GANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGL 405
           GAN+P++A++ A  GL  S  LGL A+ FA  VR  WA +FT++ +I++LTS+VLPIIGL
Sbjct: 326 GANQPDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGL 385

Query: 406 CELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLA 465
           CELGNCPQT  CGVLRG+ARPKLGANINL CFY VG+PVA+WLSF+ G+DF+GLW+GL A
Sbjct: 386 CELGNCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFA 445

Query: 466 AQGSCAMAMLMTVAWTNWEEEVERAKELTRSGSEEEVEDGD 506
           AQGSC ++ML+ +A T+WE EV RAKEL     + + +DG+
Sbjct: 446 AQGSCLISMLVVLARTDWEVEVHRAKELMTRSCDGDEDDGN 486

[Close]

BLAST of Lag0032774 vs. ExPASy Swiss-Prot
Match: Q9SLV0 (Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1)

HSP 1 Score: 535.0 bits (1377), Expect = 9.3e-151
Identity = 287/492 (58.33%), Postives = 360/492 (73.17%), Query Frame = 0


Query: 6   ASSSSVLSDQPN---SPTSTDSLRAPLISE-TPTTPTTTDKFPFLSLVLTESKSIAEIAF 65
           ++SSS+LS +     S   T     P  SE   T      ++P     L E K+I +I+ 
Sbjct: 8   SASSSLLSCKDKTHISKLETCDTDNPHYSEFRDTDSLDLKRWPSFLEGLEEVKAIGKISG 67

Query: 66  PMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQA 125
           P  + G LMYSR+MISMLFLG LG L LAGG+L+IGFANITGYSV+SGL++GMEPICGQA
Sbjct: 68  PTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGLSMGMEPICGQA 127

Query: 126 FGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLP 185
           +GAK+ KLLGL LQRTV+LLL  S+PISF W NM++ILL CGQ+++I++ A  F+L ++P
Sbjct: 128 YGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISSVAQQFLLFAIP 187

Query: 186 DLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWA 245
           DL  LS LHPLRIYLR+Q+I LP+TY   ++VL H+P+NYLLV  LEMG+ GVA+  V  
Sbjct: 188 DLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMGVAGVAIAMVLT 247

Query: 246 NFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILL 305
           N NLV  L  F+  + V+  TW  I+ + LKGW +LL LAIP+CVSVCLEWWWYE MI+L
Sbjct: 248 NLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVSVCLEWWWYEFMIIL 307

Query: 306 SGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLC 365
            G + NP++TVASMGILIQTTAL+Y+FPSSLS GVSTR+ NELGA RP +A+++ I+ L 
Sbjct: 308 CGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRPAKARVSMIISLF 367

Query: 366 SSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRG 425
            +  LGL A+VFA  VR  W  +FT D +I++LTS+ LPI+GLCELGNCPQT  CGVLRG
Sbjct: 368 CAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGNCPQTTGCGVLRG 427

Query: 426 TARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTN 485
            ARP LGANINLG FY VG+PVAI   F     F GLW GLLAAQ +CA  ML  +  T+
Sbjct: 428 CARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATCASLMLCALLRTD 487

Query: 486 WEEEVERAKELT 494
           W+ + ERA+ELT
Sbjct: 488 WKVQAERAEELT 499

[Close]

BLAST of Lag0032774 vs. ExPASy Swiss-Prot
Match: Q9SZE2 (Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1)

HSP 1 Score: 503.4 bits (1295), Expect = 3.0e-141
Identity = 251/450 (55.78%), Postives = 329/450 (73.11%), Query Frame = 0


Query: 44  PFLSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITG 103
           P ++  +TE+KS+  +AFP+ +   ++Y RS +SM FLG+LG L LA G+LAI FANITG
Sbjct: 48  PLMAEAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITG 107

Query: 104 YSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCG 163
           YSVLSGLA+GMEP+C QAFGA RFKLL L L RTV+ LL+  +PIS LWFN+ KI +   
Sbjct: 108 YSVLSGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLH 167

Query: 164 QNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLL 223
           Q+ DIA  A ++++ SLPDL+  + LHP+RIYLR+Q I  P+T  +    +FH+P N  L
Sbjct: 168 QDPDIAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFL 227

Query: 224 VSVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIP 283
           VS L +G+ GVA+ +   N  +V  L+ ++  SG++  TW   + +C +GW  LL LA P
Sbjct: 228 VSYLRLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGP 287

Query: 284 SCVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNE 343
           SCVSVCLEWWWYEIMI+L G ++NP+STVA+MG+LIQTT+ +Y+FPSSLSF VSTRVGNE
Sbjct: 288 SCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNE 347

Query: 344 LGANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIG 403
           LGANRP  AKLTA V +  +   G+ A  FA+SVR  W  +FT D +I++LT+  LPI+G
Sbjct: 348 LGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILG 407

Query: 404 LCELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLL 463
           LCE+GNCPQT  CGV+RGTARP   AN+NLG FY+VG+PVA+ L F+ G  F GLW+GLL
Sbjct: 408 LCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLL 467

Query: 464 AAQGSCAMAMLMTVAWTNWEEEVERAKELT 494
           AAQ SCA  M+  V  T+WE E ++A+ LT
Sbjct: 468 AAQISCAGLMMYVVGTTDWESEAKKAQTLT 497

[Close]

BLAST of Lag0032774 vs. ExPASy Swiss-Prot
Match: Q4PSF4 (Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 1.5e-140
Identity = 255/451 (56.54%), Postives = 333/451 (73.84%), Query Frame = 0


Query: 43  FPFLSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANIT 102
           FP ++ + +E++S+  +AFP  L   ++Y+RS ISMLFLG +G L LAGG+LAI FANIT
Sbjct: 27  FPTITELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANIT 86

Query: 103 GYSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLC 162
           GYSVL+GLA+GM+P+C QAFGA R KLL L LQRTV+ LL +S+ I  LW N+ KI++  
Sbjct: 87  GYSVLAGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYL 146

Query: 163 GQNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYL 222
            Q+  I++ A ++ILCS+PDL+  SFLHPLRIYLR+Q I  PLT       +FHIP+N+ 
Sbjct: 147 HQDPSISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFF 206

Query: 223 LVSVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAI 282
           LVS L  G  GV++ A  +N  +V  L+  + I+G+++ TW   SSEC K W  ++ LAI
Sbjct: 207 LVSYLGWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPSSECFKDWGPVVTLAI 266

Query: 283 PSCVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGN 342
           PSC+ VCLEWWWYEIM +L G +++P + VASMGILIQTT+L+YIFPSSL   VSTRVGN
Sbjct: 267 PSCIGVCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGN 326

Query: 343 ELGANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPII 402
           ELG+NRPN+A+L+AIV +  +  +GL A  FA+ V  VW  +FT D  II+LT+  LPI+
Sbjct: 327 ELGSNRPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPIL 386

Query: 403 GLCELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGL 462
           GLCELGNCPQT  CGV+RGTARP + ANINLG FY+VG PVA+ L+F+  + F GLW+GL
Sbjct: 387 GLCELGNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGL 446

Query: 463 LAAQGSCAMAMLMTVAWTNWEEEVERAKELT 494
           LAAQ  CA  ML  VA T+WE+E  RA++LT
Sbjct: 447 LAAQICCAAMMLYVVATTDWEKEAIRARKLT 477

[Close]

BLAST of Lag0032774 vs. ExPASy Swiss-Prot
Match: Q9FJ87 (Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.9e-135
Identity = 255/477 (53.46%), Postives = 340/477 (71.28%), Query Frame = 0


Query: 23  DSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLG 82
           D +  PL+ +T      +     LS+ L E+ SI +I++P+ L G  +Y RS +S+ FLG
Sbjct: 9   DEVTLPLLQKTSHLKNHSS---VLSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLG 68

Query: 83  RLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLL 142
            LG  +LAGG+LA  FANITGYS+ SGL +G+E IC QAFGA+R+  +  +++R +ILLL
Sbjct: 69  GLGDATLAGGSLAAAFANITGYSLFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLL 128

Query: 143 LASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSIN 202
           + S+P++ LW NM+KILL+  Q+  +A+EAH F+L S+PDL+A SFLHPLR+YLR+QS  
Sbjct: 129 VTSLPVTLLWMNMEKILLILKQDKKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKT 188

Query: 203 LPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFNLVGSLIVFI------IIS 262
           LPL+ C  +A   H+PI + LVS L +GI G+AL  V +NFNLV  L ++I      +  
Sbjct: 189 LPLSICTVIASFLHLPITFFLVSYLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSV 248

Query: 263 GVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGFMMNPQSTVASMG 322
              EK       + ++ W+ LL LAIPSC+SVCLEWW YEIMILL GF+++P+++VASMG
Sbjct: 249 NEDEKITEETCEDSVREWKKLLCLAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMG 308

Query: 323 ILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSFFLGLAALVFAFS 382
           ILIQ T+L+YIFP SLS GVSTRVGNELG+N+P RA+  AIVGL  S  LG  A  F  S
Sbjct: 309 ILIQITSLVYIFPHSLSLGVSTRVGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVS 368

Query: 383 VRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTARPKLGANINLGCF 442
           VR  WA  FT+D +I++LT++ LPI+GLCELGNCPQT  CGVLRG+ARPK+GANIN   F
Sbjct: 369 VRNTWAMFFTDDKEIMKLTAMALPIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAF 428

Query: 443 YIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEEEVERAKELT 494
           Y VG+PV   L+F+ G+ F+GLW+G+LAAQ +C + M+     T+WE E ERAK LT
Sbjct: 429 YAVGIPVGAVLAFWFGFGFKGLWLGMLAAQITCVIGMMAATCRTDWELEAERAKVLT 482

[Close]

BLAST of Lag0032774 vs. ExPASy TrEMBL
Match: A0A6J1HZW9 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111469149 PE=3 SV=1)

HSP 1 Score: 830.1 bits (2143), Expect = 5.2e-237
Identity = 433/525 (82.48%), Postives = 472/525 (89.90%), Query Frame = 0


Query: 1   MSDSVASSSSVLSDQPNSPTSTDSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIA 60
           MS S + S SVLSDQP  PT+T SL+APLISE       T++FP L LVLTE K IA+IA
Sbjct: 1   MSGSGSGSGSVLSDQPKIPTNTTSLKAPLISE-------TERFPNLCLVLTELKCIADIA 60

Query: 61  FPMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQ 120
            PM LVGFLMYSRSMISMLFLGRLGGLSLAGG+LAIGFANITGYSVLSGLA+GMEPICGQ
Sbjct: 61  LPMVLVGFLMYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQ 120

Query: 121 AFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSL 180
           AFGA++FKLLGLALQRTVILLL +SIPISFLWFNMKKILLLCGQNDDIA+EAHS+ILCS+
Sbjct: 121 AFGAQKFKLLGLALQRTVILLLFSSIPISFLWFNMKKILLLCGQNDDIASEAHSYILCSV 180

Query: 181 PDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVW 240
           PDLIALSFLHPLRIYLRSQSINLPLTYCAGLA++FHIPINYLLVSV + GIYGVALGAVW
Sbjct: 181 PDLIALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDWGIYGVALGAVW 240

Query: 241 ANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMIL 300
            NFNLVG LI+FII+SGVY+KTWPG+SSECLK W+SLL LAIPSC+SVCLEWWWYEIMIL
Sbjct: 241 TNFNLVGLLILFIIVSGVYKKTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMIL 300

Query: 301 LSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGL 360
           LSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPN+AKL AIVGL
Sbjct: 301 LSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGL 360

Query: 361 CSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLR 420
           C+SFFLGL+AL FAF+VR+VWA MFT DT IIELT LVLPIIGLCELGNCPQT SCGVLR
Sbjct: 361 CTSFFLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLR 420

Query: 421 GTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWT 480
           GTARPKLGANINLGCFY+VG+P+AIWLSFYGGWDF+GLWIGLLAAQGSCA+AMLM +  T
Sbjct: 421 GTARPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQGSCAIAMLMALVRT 480

Query: 481 NWEEEVERAKELTRSGSEEEVEDGDGVEENETLEDLNSMNRSVLE 526
           NWEEE ERAKELT SGS+         + +E  E LNS++RS LE
Sbjct: 481 NWEEEAERAKELTGSGSD---------DNDEKTELLNSIHRSDLE 509

[Close]

BLAST of Lag0032774 vs. ExPASy TrEMBL
Match: A0A6J1H5E0 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459742 PE=3 SV=1)

HSP 1 Score: 820.8 bits (2119), Expect = 3.1e-234
Identity = 429/529 (81.10%), Postives = 470/529 (88.85%), Query Frame = 0


Query: 6   ASSSSVLSDQPNSPTSTDSLRAPLISETPTTPTT-TDKFPFLSLVLTESKSIAEIAFPMT 65
           ASS  +LSDQPNSPT+  SL APLISE PT  TT  DK+PFL L+L+E KSIA +A PM 
Sbjct: 3   ASSVPLLSDQPNSPTTNHSLTAPLISENPTISTTVADKYPFLCLLLSECKSIAHVALPMI 62

Query: 66  LVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGA 125
            VGF MYSRSMISMLFLG+LG LSLAGG+LAIGFANITGYSVLSGLA GMEPICGQAFGA
Sbjct: 63  FVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGA 122

Query: 126 KRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLI 185
           KRFKLLGLALQRTVILLLL+SIPISFLW NMKKIL+ CGQ DDIANEAHSFILCS+PDLI
Sbjct: 123 KRFKLLGLALQRTVILLLLSSIPISFLWLNMKKILIFCGQEDDIANEAHSFILCSIPDLI 182

Query: 186 ALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFN 245
           ALSFLHPLRIYLR QSINLPLTYC GL+VLFH+PINYLLVSVL++GI+GVALGAVW NFN
Sbjct: 183 ALSFLHPLRIYLRCQSINLPLTYCVGLSVLFHVPINYLLVSVLDLGIHGVALGAVWTNFN 242

Query: 246 LVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGF 305
           LVGS+I+FI++ GVY+ +WPG SSECLK W+SLL LAIPSCVSVCLEWWWYEIMI+LSGF
Sbjct: 243 LVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCVSVCLEWWWYEIMIMLSGF 302

Query: 306 MMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSF 365
           MMNPQSTVASMGILIQTTALIYIFPSSLS GVSTRVGNELGAN+PNRAKL AIVGLC SF
Sbjct: 303 MMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGNELGANQPNRAKLAAIVGLCFSF 362

Query: 366 FLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTAR 425
           F GL+ALVFAF VR+VWATMFT DT+IIELTSLVLPIIGLCELGNCPQT +CGVLRGTAR
Sbjct: 363 FFGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTAR 422

Query: 426 PKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEE 485
           P LGANINL CFYIVG+P+AIWLSFYGGWDF+GLWIGLLAAQ SCAMAMLM +A TNWEE
Sbjct: 423 PTLGANINLVCFYIVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEE 482

Query: 486 EVERAKELTRSGSEEEVEDGDGVEENETLED-------LNSMNRSVLEV 527
           + ERA+ELTR+G   E+E+G+ VE+NET E+       LNSM    LEV
Sbjct: 483 QAERAQELTRTG-PLEIENGEDVEQNETFENDDDRNGRLNSMVERDLEV 530

[Close]

BLAST of Lag0032774 vs. ExPASy TrEMBL
Match: A0A6J1E3V0 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430342 PE=3 SV=1)

HSP 1 Score: 811.2 bits (2094), Expect = 2.5e-231
Identity = 415/499 (83.17%), Postives = 458/499 (91.78%), Query Frame = 0


Query: 5   VASSSSVLSDQPNSPT-STDSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIAFPM 64
           ++ S SVLSDQP  PT +T SL APLISE       T++FP L +VLTE K IA+IA PM
Sbjct: 1   MSGSGSVLSDQPKIPTNTTTSLNAPLISE-------TERFPNLCVVLTELKCIADIALPM 60

Query: 65  TLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFG 124
            LVGFL+YSRSMISMLFLGRLGGLSLAGG+LAIGFANITGYSVLSGLA+GMEPICGQAFG
Sbjct: 61  ILVGFLIYSRSMISMLFLGRLGGLSLAGGSLAIGFANITGYSVLSGLAVGMEPICGQAFG 120

Query: 125 AKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDL 184
           AK+FKLLGLALQRTV+LLL +S+PISFLWFNMKKILLLCGQNDDIA+EAHS+ILCS+PDL
Sbjct: 121 AKKFKLLGLALQRTVVLLLFSSVPISFLWFNMKKILLLCGQNDDIASEAHSYILCSVPDL 180

Query: 185 IALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANF 244
           IALSFLHPLRIYLRSQSINLPLTYCAGLA++FHIPINYLLVSV ++GIYGVALGAVW NF
Sbjct: 181 IALSFLHPLRIYLRSQSINLPLTYCAGLAIIFHIPINYLLVSVFDLGIYGVALGAVWTNF 240

Query: 245 NLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSG 304
           NLVG LI+FI++SGVYE TWPG+SSECLK W+SLL LAIPSC+SVCLEWWWYEIMILLSG
Sbjct: 241 NLVGLLILFIVVSGVYENTWPGMSSECLKEWKSLLELAIPSCISVCLEWWWYEIMILLSG 300

Query: 305 FMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSS 364
           FM+NPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPN+AKL AIVGLC+S
Sbjct: 301 FMINPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNKAKLAAIVGLCTS 360

Query: 365 FFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTA 424
           FFLGL+AL FAF+VR+VWA MFT DT IIELT LVLPIIGLCELGNCPQT SCGVLRGTA
Sbjct: 361 FFLGLSALAFAFNVRKVWAKMFTNDTGIIELTCLVLPIIGLCELGNCPQTTSCGVLRGTA 420

Query: 425 RPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWE 484
           RPKLGANINLGCFY+VG+P+AIWLSFYGGWDF+GLWIGLLAAQ SCA+AMLM +  TNWE
Sbjct: 421 RPKLGANINLGCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQVSCAIAMLMALVRTNWE 480

Query: 485 EEVERAKELTRSGSEEEVE 503
           E+ ERAKELT SGS+++ E
Sbjct: 481 EQAERAKELTGSGSDDDDE 492

[Close]

BLAST of Lag0032774 vs. ExPASy TrEMBL
Match: A0A6J1K4X4 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491221 PE=3 SV=1)

HSP 1 Score: 809.7 bits (2090), Expect = 7.3e-231
Identity = 423/529 (79.96%), Postives = 466/529 (88.09%), Query Frame = 0


Query: 6   ASSSSVLSDQPNSPTSTDSLRAPLISETPTTPTT-TDKFPFLSLVLTESKSIAEIAFPMT 65
           ASS  +LSDQPNSPT+  SL APLISE PT  TT  DK+PFL LVL+E KSIA +A PM 
Sbjct: 3   ASSVPLLSDQPNSPTTNHSLTAPLISENPTISTTVADKYPFLCLVLSECKSIAHVALPMI 62

Query: 66  LVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGA 125
            VGF MYSRSMISMLFLG+LG LSLAGG+LAIGFANITGYSVLSGLA GMEPICGQAFGA
Sbjct: 63  FVGFFMYSRSMISMLFLGQLGDLSLAGGSLAIGFANITGYSVLSGLASGMEPICGQAFGA 122

Query: 126 KRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLI 185
           KRFKLLGLALQRTVILLLL+SIPISF W NMKKIL+ CGQ DDIANEA SFILCS+PDLI
Sbjct: 123 KRFKLLGLALQRTVILLLLSSIPISFSWLNMKKILIFCGQEDDIANEAQSFILCSIPDLI 182

Query: 186 ALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFN 245
           ALSFLHPLRIYLR QSINLPLTYC  L+VL H+PINYLLVSVL++GI+GVALGAVW NFN
Sbjct: 183 ALSFLHPLRIYLRCQSINLPLTYCVALSVLLHVPINYLLVSVLDLGIHGVALGAVWTNFN 242

Query: 246 LVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGF 305
           LVGS+I+FI++ GVY+ +WPG SSECLK W+SLL LAIPSC+SVCLEWWWYEIM++LSGF
Sbjct: 243 LVGSVIIFILVFGVYKNSWPGFSSECLKEWKSLLRLAIPSCISVCLEWWWYEIMVMLSGF 302

Query: 306 MMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSF 365
           MMNPQSTVASMGILIQTTALIYIFPSSLS GVSTRVG ELGAN+PNRAKL AIVGLC SF
Sbjct: 303 MMNPQSTVASMGILIQTTALIYIFPSSLSVGVSTRVGKELGANQPNRAKLAAIVGLCFSF 362

Query: 366 FLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTAR 425
           F GL+ALVFAF VR+VWATMFT DT+IIELTSLVLPIIGLCELGNCPQT +CGVLRGTAR
Sbjct: 363 FFGLSALVFAFKVRKVWATMFTNDTEIIELTSLVLPIIGLCELGNCPQTTTCGVLRGTAR 422

Query: 426 PKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEE 485
           P LGANINL CFY+VG+P+AIWLSFYGGWDF+GLWIGLLAAQ SCAMAMLM +A TNWEE
Sbjct: 423 PTLGANINLVCFYMVGMPIAIWLSFYGGWDFKGLWIGLLAAQASCAMAMLMALARTNWEE 482

Query: 486 EVERAKELTRSGSEEEVEDGDGVEENETLED-------LNSMNRSVLEV 527
           + ERA+ELTR+G   E+E+G+ VE+NET E+       LNSM  S LEV
Sbjct: 483 QAERAQELTRTG-PLEIENGEDVEQNETFENDDDRNGRLNSMVESDLEV 530

[Close]

BLAST of Lag0032774 vs. ExPASy TrEMBL
Match: A0A0A0KKM4 (Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G155540 PE=3 SV=1)

HSP 1 Score: 791.2 bits (2042), Expect = 2.7e-225
Identity = 403/492 (81.91%), Postives = 445/492 (90.45%), Query Frame = 0


Query: 24  SLRAPLISETPTTPTTTDKFPF-LSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLG 83
           +L+ PLI ET T      KFP+  S VL+E+KSIA+IAFPM LVGFLMYSRSMISMLFLG
Sbjct: 12  TLKTPLIEETET------KFPYRFSHVLSEAKSIADIAFPMILVGFLMYSRSMISMLFLG 71

Query: 84  RLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLL 143
           RLGGLSLAGG+LAIGFANITGYS+LSGLA+GMEPICGQAFGAKRFKLLGL LQRT+ILLL
Sbjct: 72  RLGGLSLAGGSLAIGFANITGYSLLSGLAMGMEPICGQAFGAKRFKLLGLTLQRTIILLL 131

Query: 144 LASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSIN 203
           ++S+PISFLWFNMKKILL CGQ+ DIANEAHS+ILCSLPDL+ALSFLHPLRIYLRSQSIN
Sbjct: 132 ISSLPISFLWFNMKKILLFCGQDVDIANEAHSYILCSLPDLVALSFLHPLRIYLRSQSIN 191

Query: 204 LPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKT 263
           LPLTYCA LA+LFHIPINY  V V E GI GVALGAVW NFN VGSL+VF++ SGVY+KT
Sbjct: 192 LPLTYCAILAILFHIPINYFFVCVFEWGIRGVALGAVWTNFNFVGSLVVFVLFSGVYKKT 251

Query: 264 WPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTT 323
           WPG+SS+CLK W+SLLGLAIPSC+SVCLEWWWYEIMILLSGFM+NPQSTVASMGILIQTT
Sbjct: 252 WPGMSSDCLKEWKSLLGLAIPSCISVCLEWWWYEIMILLSGFMLNPQSTVASMGILIQTT 311

Query: 324 ALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWA 383
           ALIYIFPSSLSFGVSTRVGNELGAN PN+AKL AIVGLC SFFLG++AL+FAF +R+VWA
Sbjct: 312 ALIYIFPSSLSFGVSTRVGNELGANHPNKAKLAAIVGLCISFFLGISALLFAFKIRKVWA 371

Query: 384 TMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLP 443
           TMFTED QIIELTSL+LPIIGLCELGNCPQT SCGVLRGTARPKLGANINLGCFY+VG+P
Sbjct: 372 TMFTEDIQIIELTSLILPIIGLCELGNCPQTTSCGVLRGTARPKLGANINLGCFYMVGMP 431

Query: 444 VAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEEEVERAKELTRSGSEEEVE 503
           VAIWLSFYGGWDF+GLWIGLLAAQ SCAM MLM +  TNWEE+ ERAKELT++G  EE+E
Sbjct: 432 VAIWLSFYGGWDFKGLWIGLLAAQASCAMTMLMVLTRTNWEEQAERAKELTKNGL-EEIE 491

Query: 504 DGDGVEENETLE 515
           D +  EE E  E
Sbjct: 492 DDEEEEEEENQE 496

[Close]

BLAST of Lag0032774 vs. TAIR 10
Match: AT4G23030.1 (MATE efflux family protein )

HSP 1 Score: 633.6 bits (1633), Expect = 1.4e-181
Identity = 323/461 (70.07%), Postives = 385/461 (83.51%), Query Frame = 0


Query: 46  LSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLGRLGGLS-LAGGALAIGFANITGY 105
           LS  + E+KSIA+I+ P+ L G L+YSRSMISMLFLGRL  LS L+GG+LA+GFANITGY
Sbjct: 26  LSSSIQEAKSIAKISLPLILTGLLLYSRSMISMLFLGRLNDLSALSGGSLALGFANITGY 85

Query: 106 SVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQ 165
           S+LSGL+IGMEPIC QAFGAKRFKLLGLALQRT +LLLL S+PIS LW N+KKILL  GQ
Sbjct: 86  SLLSGLSIGMEPICVQAFGAKRFKLLGLALQRTTLLLLLCSLPISILWLNIKKILLFFGQ 145

Query: 166 NDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLV 225
           +++I+N+A  FIL SLPDLI  SFLHP+RIYLRSQSI LPLTY A  AVL HIPINYLLV
Sbjct: 146 DEEISNQAEIFILFSLPDLILQSFLHPIRIYLRSQSITLPLTYSAFFAVLLHIPINYLLV 205

Query: 226 SVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPS 285
           S L +G+ GVALGA+W N NL+G LI++I+ SGVY+KTW G S +C KGWRSL+ LAIPS
Sbjct: 206 SSLGLGLKGVALGAIWTNVNLLGFLIIYIVFSGVYQKTWGGFSMDCFKGWRSLMKLAIPS 265

Query: 286 CVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNEL 345
           CVSVCLEWWWYEIMILL G ++NPQ+TVASMGILIQTTALIYIFPSSLS  VSTRVGNEL
Sbjct: 266 CVSVCLEWWWYEIMILLCGLLLNPQATVASMGILIQTTALIYIFPSSLSISVSTRVGNEL 325

Query: 346 GANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGL 405
           GAN+P++A++ A  GL  S  LGL A+ FA  VR  WA +FT++ +I++LTS+VLPIIGL
Sbjct: 326 GANQPDKARIAARTGLSLSLGLGLLAMFFALMVRNCWARLFTDEEEIVKLTSMVLPIIGL 385

Query: 406 CELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLA 465
           CELGNCPQT  CGVLRG+ARPKLGANINL CFY VG+PVA+WLSF+ G+DF+GLW+GL A
Sbjct: 386 CELGNCPQTTLCGVLRGSARPKLGANINLCCFYFVGMPVAVWLSFFSGFDFKGLWLGLFA 445

Query: 466 AQGSCAMAMLMTVAWTNWEEEVERAKELTRSGSEEEVEDGD 506
           AQGSC ++ML+ +A T+WE EV RAKEL     + + +DG+
Sbjct: 446 AQGSCLISMLVVLARTDWEVEVHRAKELMTRSCDGDEDDGN 486

[Close]

BLAST of Lag0032774 vs. TAIR 10
Match: AT1G58340.1 (MATE efflux family protein )

HSP 1 Score: 535.0 bits (1377), Expect = 6.6e-152
Identity = 287/492 (58.33%), Postives = 360/492 (73.17%), Query Frame = 0


Query: 6   ASSSSVLSDQPN---SPTSTDSLRAPLISE-TPTTPTTTDKFPFLSLVLTESKSIAEIAF 65
           ++SSS+LS +     S   T     P  SE   T      ++P     L E K+I +I+ 
Sbjct: 8   SASSSLLSCKDKTHISKLETCDTDNPHYSEFRDTDSLDLKRWPSFLEGLEEVKAIGKISG 67

Query: 66  PMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQA 125
           P  + G LMYSR+MISMLFLG LG L LAGG+L+IGFANITGYSV+SGL++GMEPICGQA
Sbjct: 68  PTAMTGLLMYSRAMISMLFLGYLGELELAGGSLSIGFANITGYSVISGLSMGMEPICGQA 127

Query: 126 FGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLP 185
           +GAK+ KLLGL LQRTV+LLL  S+PISF W NM++ILL CGQ+++I++ A  F+L ++P
Sbjct: 128 YGAKQMKLLGLTLQRTVLLLLSCSVPISFSWLNMRRILLWCGQDEEISSVAQQFLLFAIP 187

Query: 186 DLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWA 245
           DL  LS LHPLRIYLR+Q+I LP+TY   ++VL H+P+NYLLV  LEMG+ GVA+  V  
Sbjct: 188 DLFLLSLLHPLRIYLRTQNITLPVTYSTAVSVLLHVPLNYLLVVKLEMGVAGVAIAMVLT 247

Query: 246 NFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILL 305
           N NLV  L  F+  + V+  TW  I+ + LKGW +LL LAIP+CVSVCLEWWWYE MI+L
Sbjct: 248 NLNLVVLLSSFVYFTSVHSDTWVPITIDSLKGWSALLSLAIPTCVSVCLEWWWYEFMIIL 307

Query: 306 SGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLC 365
            G + NP++TVASMGILIQTTAL+Y+FPSSLS GVSTR+ NELGA RP +A+++ I+ L 
Sbjct: 308 CGLLANPRATVASMGILIQTTALVYVFPSSLSLGVSTRISNELGAKRPAKARVSMIISLF 367

Query: 366 SSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRG 425
            +  LGL A+VFA  VR  W  +FT D +I++LTS+ LPI+GLCELGNCPQT  CGVLRG
Sbjct: 368 CAIALGLMAMVFAVLVRHHWGRLFTTDAEILQLTSIALPIVGLCELGNCPQTTGCGVLRG 427

Query: 426 TARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTN 485
            ARP LGANINLG FY VG+PVAI   F     F GLW GLLAAQ +CA  ML  +  T+
Sbjct: 428 CARPTLGANINLGSFYFVGMPVAILFGFVFKQGFPGLWFGLLAAQATCASLMLCALLRTD 487

Query: 486 WEEEVERAKELT 494
           W+ + ERA+ELT
Sbjct: 488 WKVQAERAEELT 499

[Close]

BLAST of Lag0032774 vs. TAIR 10
Match: AT4G29140.1 (MATE efflux family protein )

HSP 1 Score: 503.4 bits (1295), Expect = 2.1e-142
Identity = 251/450 (55.78%), Postives = 329/450 (73.11%), Query Frame = 0


Query: 44  PFLSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANITG 103
           P ++  +TE+KS+  +AFP+ +   ++Y RS +SM FLG+LG L LA G+LAI FANITG
Sbjct: 48  PLMAEAVTEAKSLFTLAFPIAVTALVLYLRSAVSMFFLGQLGDLELAAGSLAIAFANITG 107

Query: 104 YSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLCG 163
           YSVLSGLA+GMEP+C QAFGA RFKLL L L RTV+ LL+  +PIS LWFN+ KI +   
Sbjct: 108 YSVLSGLALGMEPLCSQAFGAHRFKLLSLTLHRTVVFLLVCCVPISVLWFNVGKISVYLH 167

Query: 164 QNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYLL 223
           Q+ DIA  A ++++ SLPDL+  + LHP+RIYLR+Q I  P+T  +    +FH+P N  L
Sbjct: 168 QDPDIAKLAQTYLIFSLPDLLTNTLLHPIRIYLRAQGIIHPVTLASLSGAVFHLPANLFL 227

Query: 224 VSVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAIP 283
           VS L +G+ GVA+ +   N  +V  L+ ++  SG++  TW   + +C +GW  LL LA P
Sbjct: 228 VSYLRLGLTGVAVASSITNIFVVAFLVCYVWASGLHAPTWTDPTRDCFRGWAPLLRLAGP 287

Query: 284 SCVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGNE 343
           SCVSVCLEWWWYEIMI+L G ++NP+STVA+MG+LIQTT+ +Y+FPSSLSF VSTRVGNE
Sbjct: 288 SCVSVCLEWWWYEIMIVLCGLLVNPRSTVAAMGVLIQTTSFLYVFPSSLSFAVSTRVGNE 347

Query: 344 LGANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPIIG 403
           LGANRP  AKLTA V +  +   G+ A  FA+SVR  W  +FT D +I++LT+  LPI+G
Sbjct: 348 LGANRPKTAKLTATVAIVFAAVTGIIAAAFAYSVRNAWGRIFTGDKEILQLTAAALPILG 407

Query: 404 LCELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGLL 463
           LCE+GNCPQT  CGV+RGTARP   AN+NLG FY+VG+PVA+ L F+ G  F GLW+GLL
Sbjct: 408 LCEIGNCPQTVGCGVVRGTARPSTAANVNLGAFYLVGMPVAVGLGFWAGIGFNGLWVGLL 467

Query: 464 AAQGSCAMAMLMTVAWTNWEEEVERAKELT 494
           AAQ SCA  M+  V  T+WE E ++A+ LT
Sbjct: 468 AAQISCAGLMMYVVGTTDWESEAKKAQTLT 497

[Close]

BLAST of Lag0032774 vs. TAIR 10
Match: AT5G19700.1 (MATE efflux family protein )

HSP 1 Score: 501.1 bits (1289), Expect = 1.1e-141
Identity = 255/451 (56.54%), Postives = 333/451 (73.84%), Query Frame = 0


Query: 43  FPFLSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLGRLGGLSLAGGALAIGFANIT 102
           FP ++ + +E++S+  +AFP  L   ++Y+RS ISMLFLG +G L LAGG+LAI FANIT
Sbjct: 27  FPTITELKSEARSLFSLAFPTILAALILYARSAISMLFLGHIGELELAGGSLAIAFANIT 86

Query: 103 GYSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLLLASIPISFLWFNMKKILLLC 162
           GYSVL+GLA+GM+P+C QAFGA R KLL L LQRTV+ LL +S+ I  LW N+ KI++  
Sbjct: 87  GYSVLAGLALGMDPLCSQAFGAGRPKLLSLTLQRTVLFLLTSSVVIVALWLNLGKIMIYL 146

Query: 163 GQNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSINLPLTYCAGLAVLFHIPINYL 222
            Q+  I++ A ++ILCS+PDL+  SFLHPLRIYLR+Q I  PLT       +FHIP+N+ 
Sbjct: 147 HQDPSISSLAQTYILCSIPDLLTNSFLHPLRIYLRAQGITSPLTLATLAGTIFHIPMNFF 206

Query: 223 LVSVLEMGIYGVALGAVWANFNLVGSLIVFIIISGVYEKTWPGISSECLKGWRSLLGLAI 282
           LVS L  G  GV++ A  +N  +V  L+  + I+G+++ TW   SSEC K W  ++ LAI
Sbjct: 207 LVSYLGWGFMGVSMAAAASNLLVVIFLVAHVWIAGLHQPTWTRPSSECFKDWGPVVTLAI 266

Query: 283 PSCVSVCLEWWWYEIMILLSGFMMNPQSTVASMGILIQTTALIYIFPSSLSFGVSTRVGN 342
           PSC+ VCLEWWWYEIM +L G +++P + VASMGILIQTT+L+YIFPSSL   VSTRVGN
Sbjct: 267 PSCIGVCLEWWWYEIMTVLCGLLIDPSTPVASMGILIQTTSLLYIFPSSLGLAVSTRVGN 326

Query: 343 ELGANRPNRAKLTAIVGLCSSFFLGLAALVFAFSVRRVWATMFTEDTQIIELTSLVLPII 402
           ELG+NRPN+A+L+AIV +  +  +GL A  FA+ V  VW  +FT D  II+LT+  LPI+
Sbjct: 327 ELGSNRPNKARLSAIVAVSFAGVMGLTASAFAWGVSDVWGWIFTNDVAIIKLTAAALPIL 386

Query: 403 GLCELGNCPQTASCGVLRGTARPKLGANINLGCFYIVGLPVAIWLSFYGGWDFRGLWIGL 462
           GLCELGNCPQT  CGV+RGTARP + ANINLG FY+VG PVA+ L+F+  + F GLW+GL
Sbjct: 387 GLCELGNCPQTVGCGVVRGTARPSMAANINLGAFYLVGTPVAVGLTFWAAYGFCGLWVGL 446

Query: 463 LAAQGSCAMAMLMTVAWTNWEEEVERAKELT 494
           LAAQ  CA  ML  VA T+WE+E  RA++LT
Sbjct: 447 LAAQICCAAMMLYVVATTDWEKEAIRARKLT 477

[Close]

BLAST of Lag0032774 vs. TAIR 10
Match: AT5G52050.1 (MATE efflux family protein )

HSP 1 Score: 484.2 bits (1245), Expect = 1.3e-136
Identity = 255/477 (53.46%), Postives = 340/477 (71.28%), Query Frame = 0


Query: 23  DSLRAPLISETPTTPTTTDKFPFLSLVLTESKSIAEIAFPMTLVGFLMYSRSMISMLFLG 82
           D +  PL+ +T      +     LS+ L E+ SI +I++P+ L G  +Y RS +S+ FLG
Sbjct: 9   DEVTLPLLQKTSHLKNHSS---VLSVFLNEAISICKISYPLVLTGLFLYVRSFVSLSFLG 68

Query: 83  RLGGLSLAGGALAIGFANITGYSVLSGLAIGMEPICGQAFGAKRFKLLGLALQRTVILLL 142
            LG  +LAGG+LA  FANITGYS+ SGL +G+E IC QAFGA+R+  +  +++R +ILLL
Sbjct: 69  GLGDATLAGGSLAAAFANITGYSLFSGLTMGVESICSQAFGARRYNYVCASVKRGIILLL 128

Query: 143 LASIPISFLWFNMKKILLLCGQNDDIANEAHSFILCSLPDLIALSFLHPLRIYLRSQSIN 202
           + S+P++ LW NM+KILL+  Q+  +A+EAH F+L S+PDL+A SFLHPLR+YLR+QS  
Sbjct: 129 VTSLPVTLLWMNMEKILLILKQDKKLASEAHIFLLYSVPDLVAQSFLHPLRVYLRTQSKT 188

Query: 203 LPLTYCAGLAVLFHIPINYLLVSVLEMGIYGVALGAVWANFNLVGSLIVFI------IIS 262
           LPL+ C  +A   H+PI + LVS L +GI G+AL  V +NFNLV  L ++I      +  
Sbjct: 189 LPLSICTVIASFLHLPITFFLVSYLGLGIKGIALSGVVSNFNLVAFLFLYICFFEDKLSV 248

Query: 263 GVYEKTWPGISSECLKGWRSLLGLAIPSCVSVCLEWWWYEIMILLSGFMMNPQSTVASMG 322
              EK       + ++ W+ LL LAIPSC+SVCLEWW YEIMILL GF+++P+++VASMG
Sbjct: 249 NEDEKITEETCEDSVREWKKLLCLAIPSCISVCLEWWCYEIMILLCGFLLDPKASVASMG 308

Query: 323 ILIQTTALIYIFPSSLSFGVSTRVGNELGANRPNRAKLTAIVGLCSSFFLGLAALVFAFS 382
           ILIQ T+L+YIFP SLS GVSTRVGNELG+N+P RA+  AIVGL  S  LG  A  F  S
Sbjct: 309 ILIQITSLVYIFPHSLSLGVSTRVGNELGSNQPKRARRAAIVGLGLSIALGFTAFAFTVS 368

Query: 383 VRRVWATMFTEDTQIIELTSLVLPIIGLCELGNCPQTASCGVLRGTARPKLGANINLGCF 442
           VR  WA  FT+D +I++LT++ LPI+GLCELGNCPQT  CGVLRG+ARPK+GANIN   F
Sbjct: 369 VRNTWAMFFTDDKEIMKLTAMALPIVGLCELGNCPQTTGCGVLRGSARPKIGANINGVAF 428

Query: 443 YIVGLPVAIWLSFYGGWDFRGLWIGLLAAQGSCAMAMLMTVAWTNWEEEVERAKELT 494
           Y VG+PV   L+F+ G+ F+GLW+G+LAAQ +C + M+     T+WE E ERAK LT
Sbjct: 429 YAVGIPVGAVLAFWFGFGFKGLWLGMLAAQITCVIGMMAATCRTDWELEAERAKVLT 482

The following BLAST results are available for this feature:

BLAST of Lag0032774 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022970176.1	1.1e-236	82.48	protein DETOXIFICATION 49-like [Cucurbita maxima]	[more]
XP_022958539.1	6.5e-234	81.10	protein DETOXIFICATION 49-like [Cucurbita moschata]	[more]
KAG7016948.1	7.2e-233	84.17	Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6606121.1	9.4e-233	80.91	Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG6579480.1	2.7e-232	83.97	Protein DETOXIFICATION 49, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Lag0032774 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O82752	1.9e-180	70.07	Protein DETOXIFICATION 49 OS=Arabidopsis thaliana OX=3702 GN=DTX49 PE=2 SV=1	[more]
Q9SLV0	9.3e-151	58.33	Protein DETOXIFICATION 48 OS=Arabidopsis thaliana OX=3702 GN=DTX48 PE=2 SV=1	[more]
Q9SZE2	3.0e-141	55.78	Protein DETOXIFICATION 51 OS=Arabidopsis thaliana OX=3702 GN=DTX51 PE=2 SV=1	[more]
Q4PSF4	1.5e-140	56.54	Protein DETOXIFICATION 52 OS=Arabidopsis thaliana OX=3702 GN=DTX52 PE=2 SV=1	[more]
Q9FJ87	1.9e-135	53.46	Protein DETOXIFICATION 50 OS=Arabidopsis thaliana OX=3702 GN=DTX50 PE=2 SV=1	[more]

BLAST of Lag0032774 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HZW9	5.2e-237	82.48	Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111469149 PE=3 SV=1	[more]
A0A6J1H5E0	3.1e-234	81.10	Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111459742 PE=3 SV=1	[more]
A0A6J1E3V0	2.5e-231	83.17	Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111430342 PE=3 SV=1	[more]
A0A6J1K4X4	7.3e-231	79.96	Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111491221 PE=3 SV=1	[more]
A0A0A0KKM4	2.7e-225	81.91	Protein DETOXIFICATION OS=Cucumis sativus OX=3659 GN=Csa_5G155540 PE=3 SV=1	[more]

BLAST of Lag0032774 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G23030.1	1.4e-181	70.07	MATE efflux family protein	[more]
AT1G58340.1	6.6e-152	58.33	MATE efflux family protein	[more]
AT4G29140.1	2.1e-142	55.78	MATE efflux family protein	[more]
AT5G19700.1	1.1e-141	56.54	MATE efflux family protein	[more]
AT5G52050.1	1.3e-136	53.46	MATE efflux family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002528	Multi antimicrobial extrusion protein	PFAM	PF01554	MatE	coord: 62..222 e-value: 5.1E-36 score: 123.8 coord: 283..445 e-value: 1.7E-34 score: 118.8
IPR002528	Multi antimicrobial extrusion protein	TIGRFAM	TIGR00797	TIGR00797	coord: 69..459 e-value: 1.4E-62 score: 209.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 489..526
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..36
None	No IPR available	PANTHER	PTHR11206:SF387	PROTEIN DETOXIFICATION	coord: 37..503
None	No IPR available	PANTHER	PTHR11206	MULTIDRUG RESISTANCE PROTEIN	coord: 37..503
IPR045069	Multidrug and toxic compound extrusion family, eukaryotic	CDD	cd13132	MATE_eukaryotic	coord: 52..488 e-value: 7.80539E-173 score: 492.858

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032774.1	Lag0032774.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1990961	xenobiotic detoxification by transmembrane export across the plasma membrane
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0015297	antiporter activity
molecular_function	GO:0042910	xenobiotic transmembrane transporter activity