Lag0032281 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0032281
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr11: 29469201 .. 29469776 (-)
RNA-Seq ExpressionLag0032281
SyntenyLag0032281
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAAGAAAATATCGGAACTGAAGATCTGGAGAAAGCGATCTTAGTTCTATGGAGTTTATGGAACCAGAGAAACCAATCAATCCAGCCGAATCAAGATCCCGATTTCAAATATCTAACAAGGGCGATTAATGCAAACATCAAGGAGGAGAAAAAGAGAGTTTCCGCTAACCTGTTGAGCGGTCTCGGGTGGATTTTGCGTGACTGTACAGGATCTCCCATCTGTGGAGGATTGAAGAGGATCAACTCTAGCTGGTCCATCAAAATGCAGGAGATGAAGGCTATTCTAGAAGGGCTGAAGAGTTTACCTACTCTGCGAGGCCGAGCAGTCCAAGTCGATTTGAAATTCCCTACTATTACAGTGGAATCAGACGCCGTCGGAGTGATCAAAATGCTCCACGGCGAAGAAGAGGATCTTTCGGAAGTGGCGTTCTTAGCGGCCGAGATCTCTCTTGCGAAATCCTGGTTTGAGGATATCCGTTTTGTTCATGCTCCCCGTGGATGTAATTTTTTCGTTCATAATTTGGCGCGAATCGCGTGTTGTAGCTCCGCCTTCTCAGGTTGTTGGGTTCATTAA

mRNA sequence

ATGGAAGAAAATATCGGAACTGAAGATCTGGAGAAAGCGATCTTAGTTCTATGGAGTTTATGGAACCAGAGAAACCAATCAATCCAGCCGAATCAAGATCCCGATTTCAAATATCTAACAAGGGCGATTAATGCAAACATCAAGGAGGAGAAAAAGAGAGTTTCCGCTAACCTGTTGAGCGGTCTCGGGTGGATTTTGCGTGACTGTACAGGATCTCCCATCTGTGGAGGATTGAAGAGGATCAACTCTAGCTGGTCCATCAAAATGCAGGAGATGAAGGCTATTCTAGAAGGGCTGAAGAGTTTACCTACTCTGCGAGGCCGAGCAGTCCAAGTCGATTTGAAATTCCCTACTATTACAGTGGAATCAGACGCCGTCGGAGTGATCAAAATGCTCCACGGCGAAGAAGAGGATCTTTCGGAAGTGGCGTTCTTAGCGGCCGAGATCTCTCTTGCGAAATCCTGGTTTGAGGATATCCGTTTTGTTCATGCTCCCCGTGGATGTAATTTTTTCGTTCATAATTTGGCGCGAATCGCGTGTTGTAGCTCCGCCTTCTCAGGTTGTTGGGTTCATTAA

Coding sequence (CDS)

ATGGAAGAAAATATCGGAACTGAAGATCTGGAGAAAGCGATCTTAGTTCTATGGAGTTTATGGAACCAGAGAAACCAATCAATCCAGCCGAATCAAGATCCCGATTTCAAATATCTAACAAGGGCGATTAATGCAAACATCAAGGAGGAGAAAAAGAGAGTTTCCGCTAACCTGTTGAGCGGTCTCGGGTGGATTTTGCGTGACTGTACAGGATCTCCCATCTGTGGAGGATTGAAGAGGATCAACTCTAGCTGGTCCATCAAAATGCAGGAGATGAAGGCTATTCTAGAAGGGCTGAAGAGTTTACCTACTCTGCGAGGCCGAGCAGTCCAAGTCGATTTGAAATTCCCTACTATTACAGTGGAATCAGACGCCGTCGGAGTGATCAAAATGCTCCACGGCGAAGAAGAGGATCTTTCGGAAGTGGCGTTCTTAGCGGCCGAGATCTCTCTTGCGAAATCCTGGTTTGAGGATATCCGTTTTGTTCATGCTCCCCGTGGATGTAATTTTTTCGTTCATAATTTGGCGCGAATCGCGTGTTGTAGCTCCGCCTTCTCAGGTTGTTGGGTTCATTAA

Protein sequence

MEENIGTEDLEKAILVLWSLWNQRNQSIQPNQDPDFKYLTRAINANIKEEKKRVSANLLSGLGWILRDCTGSPICGGLKRINSSWSIKMQEMKAILEGLKSLPTLRGRAVQVDLKFPTITVESDAVGVIKMLHGEEEDLSEVAFLAAEISLAKSWFEDIRFVHAPRGCNFFVHNLARIACCSSAFSGCWVH
Homology
BLAST of Lag0032281 vs. NCBI nr
Match: XP_015385738.1 (uncharacterized protein LOC107177034 [Citrus sinensis])

HSP 1 Score: 68.9 bits (167), Expect = 5.3e-08
Identity = 48/214 (22.43%), Postives = 86/214 (40.19%), Query Frame = 0

Query: 1   MEENIGTEDLEKAILVLWSLWNQRNQSI--------------------------QPNQDP 60
           M + +   D+E  + + W++W  RN+ +                          QP  DP
Sbjct: 74  MAKKLTKSDIEVMVAICWTIWYGRNKFLFEGKKMEPLLTISRAEAVVEAYRRVKQPTLDP 133

Query: 61  DFKYLTRAINANIKEEKKRVSANL---------LSGLGWILRDCTGSPICGGLKRINSSW 120
           + KY ++               N+          +GLG ++RD +       +K    + 
Sbjct: 134 EAKYSSKNQQVWSPPPDNTFKVNVDAAVNFDRQKAGLGVVIRDSSNKVKVAAVKSTLFTG 193

Query: 121 SIKMQEMKAILEGLKSLPTLRGRAVQVDLKFPTITVESDAVGVIKMLHGEEEDLSEVAFL 180
            ++  E +A+  GL          V     F  I VESD   V+K+++  E   +E+ ++
Sbjct: 194 DVQTAEAEAVEWGL---------VVAKSAAFQCIMVESDCQEVVKLINNNEGSRTEIMWV 253

BLAST of Lag0032281 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-07
Identity = 56/220 (25.45%), Postives = 92/220 (41.82%), Query Frame = 0

Query: 3   ENIGTEDLEKAILVLWSLWNQRNQSI---------------------QPNQDPDFK---- 62
           +  G E+  +++++ W +W  RN+SI                        +D + K    
Sbjct: 2   DKAGEEERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSA 61

Query: 63  ----YLTRAINANIKEEKKRVSANL--------------LSGLGWILRDCTGSPICGGLK 122
               +L R I  N     K  ++N                 G+GWILRD  G  I    +
Sbjct: 62  NKDLHLIRRIGDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCR 121

Query: 123 RINSSWSIKMQEMKAILEGLKSLPTLRGRAVQVDLKFPTITVESDAVGVIKMLHGEEEDL 180
            I +  +I   E+ AI EGL+++     R +Q +   P I +ESD++  I +LH + +D 
Sbjct: 122 IIRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRP-IHLESDSLEAIHLLHRQCQDQ 181

BLAST of Lag0032281 vs. NCBI nr
Match: XP_027109195.1 (uncharacterized protein LOC113729068 [Coffea arabica])

HSP 1 Score: 65.5 bits (158), Expect = 5.8e-07
Identity = 57/218 (26.15%), Postives = 91/218 (41.74%), Query Frame = 0

Query: 8   EDLEKAILVLWSLWNQRNQSI-----------------------QPNQDPDFKYLTRAIN 67
           E++E   ++LW+LWN RN ++                       + N++P     T AI 
Sbjct: 391 EEMELVAVILWNLWNNRNGAVFDGSYKDPLSLVSFSLHFLSQFREANRNPMAAEHTTAIQ 450

Query: 68  ANIKEEKKR-----VSANL---------LSGLGWILRDCTGSPICGGLKRINSSWSIKMQ 127
            ++  + KR     V AN          +SG+G ++RD  G+ + G  K+I   ++ ++ 
Sbjct: 451 QSVLSQWKRPPAGYVKANFDGAIFVEGDISGIGVVIRDAEGNFMAGLAKQIPGIFAPEVI 510

Query: 128 EMKAILEGLKSLPTLRGRAVQVDLKFPTITVESDAVGVIKMLHGEEEDLSEVAFLAAEIS 187
           E  A    ++ L         +DL  P I +E D + V+KML   E D S    L  +I 
Sbjct: 511 ESYAARAAVELL---------LDLHLPMIILEGDCLKVVKMLQTTETDASACGMLVDDIL 570

Query: 188 LAKSWFEDIRFVHAPRGCNFFVHNLARIACCSSAFSGC 189
                F        PR  N   H +A+ AC  S  +GC
Sbjct: 571 RDIQSFATWEASWVPRQLNNVAHRVAKYAC--SISNGC 597

BLAST of Lag0032281 vs. NCBI nr
Match: XP_027158404.1 (uncharacterized protein LOC113760026 [Coffea eugenioides])

HSP 1 Score: 65.5 bits (158), Expect = 5.8e-07
Identity = 57/218 (26.15%), Postives = 91/218 (41.74%), Query Frame = 0

Query: 8   EDLEKAILVLWSLWNQRNQSI-----------------------QPNQDPDFKYLTRAIN 67
           E++E   ++LW+LWN RN ++                       + N++P     T AI 
Sbjct: 391 EEMELVAVILWNLWNNRNGAVFDGSYKDPLSLVSFSLHFLSQFREANRNPMAAEHTTAIQ 450

Query: 68  ANIKEEKKR-----VSANL---------LSGLGWILRDCTGSPICGGLKRINSSWSIKMQ 127
            ++  + KR     V AN          +SG+G ++RD  G+ + G  K+I   ++ ++ 
Sbjct: 451 QSVLSQWKRPPAGYVKANFDGAIFVEGDISGIGVVIRDAEGNFMAGLAKQIPGIFAPEVI 510

Query: 128 EMKAILEGLKSLPTLRGRAVQVDLKFPTITVESDAVGVIKMLHGEEEDLSEVAFLAAEIS 187
           E  A    ++ L         +DL  P I +E D + V+KML   E D S    L  +I 
Sbjct: 511 ESYAARAAVELL---------LDLHLPMIILEGDCLKVVKMLQTTETDASACGMLVDDIL 570

Query: 188 LAKSWFEDIRFVHAPRGCNFFVHNLARIACCSSAFSGC 189
                F        PR  N   H +A+ AC  S  +GC
Sbjct: 571 RDIQSFATWEASWVPRQLNNVAHRVAKYAC--SISNGC 597

BLAST of Lag0032281 vs. NCBI nr
Match: XP_024164491.1 (uncharacterized protein LOC112171562 [Rosa chinensis])

HSP 1 Score: 63.5 bits (153), Expect = 2.2e-06
Identity = 53/205 (25.85%), Postives = 82/205 (40.00%), Query Frame = 0

Query: 4   NIGTEDLEKAILVLWSLWNQRN-----QSIQPNQD---------PDFKYLTRAINANIKE 63
           N+  E  EK ++++W LW  RN      S Q + D          +F+   +     I+ 
Sbjct: 54  NLKLETFEKLLMIIWGLWKNRNTKLWEDSAQSSNDIMLGCLTWLEEFRQTRQVPATQIQN 113

Query: 64  EKKR---------------VSANLLSGLGWILRDCTGSPICGGLKRINSSWSIKMQEMKA 123
             K                V+       G +LR  TGS I   +K +    S +  E+ A
Sbjct: 114 VAKNWKPAEIPKLNVDGAFVTQAAYGETGGVLRSSTGSFIAAFMKPVQHVNSAQQVELLA 173

Query: 124 ILEGLKSLPTLRGRAVQVDLKFPTITVESDAVGVIKMLHGEEEDLSEVAFLAAEISLAKS 180
           I EGL  L TLR              +ESD +  I+ L   + +LSE++ L  +I     
Sbjct: 174 IREGLNFLKTLR---------LQQAIIESDCLLAIQDLARTDVNLSELSNLVQDIQQTIQ 233

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_015385738.15.3e-0822.43uncharacterized protein LOC107177034 [Citrus sinensis][more]
XP_022156777.11.5e-0725.45uncharacterized protein LOC111023608 [Momordica charantia][more]
XP_027109195.15.8e-0726.15uncharacterized protein LOC113729068 [Coffea arabica][more]
XP_027158404.15.8e-0726.15uncharacterized protein LOC113760026 [Coffea eugenioides][more]
XP_024164491.12.2e-0625.85uncharacterized protein LOC112171562 [Rosa chinensis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 46..180
e-value: 4.8E-8
score: 35.1
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 58..177
e-value: 1.2E-15
score: 57.4
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 60..177
e-value: 1.43043E-15
score: 67.7244
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 60..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0032281.1Lag0032281.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity