Lag0032085 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0032085 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 282.0 bits (720), Expect = 1.0e-71
Identity = 191/638 (29.94%), Postives = 260/638 (40.75%), Query Frame = 0

Query: 1    MNKKESIQPWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKY 60
            +N  + I+ WN T I  IPK +QPR +SD+RPI LCN++YK ++K I NRLK ++  +  
Sbjct: 637  LNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLCNVSYKIISKSITNRLKNVIGLVIS 696

Query: 61   ECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLE 120
            + Q AF+P R+I+DN+IIGHE LH + + + G  G AALKLD+SKA+DRVEW YL  ++ 
Sbjct: 697  DAQSAFVPSRAISDNVIIGHECLHTINSCKSGLIGMAALKLDLSKAFDRVEWTYLECIMR 756

Query: 121  RLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVM 180
            ++GF+E  I  I+ CIS+  FSI +NG   G  + SRGIRQGDPLS YLFLLC +GLS +
Sbjct: 757  KMGFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGIRQGDPLSPYLFLLCAEGLSAL 816

Query: 181  L----------------------------------------------------------- 240
            +                                                           
Sbjct: 817  INHENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLESECLALRRLLDSYGRASGQCI 876

Query: 241  -----------------QTTYSC------------------------------------- 300
                             Q    C                                     
Sbjct: 877  NFSKSALLFSPNVHPERQQYLQCILNVKLVSHFGNYLGLPSHFTRRRGESRKLHWMKWGR 936

Query: 301  ---------------------SISEQVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSH 360
                                  +++ VWR L++PNL + K+LK +YF  ++L   S  S 
Sbjct: 937  MCYPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSK 996

Query: 361  SSYFWKGFLWGMDLLQKGTRRNLGN----------------------------------- 403
            SSYFWKGFLWG DLL KG R  +GN                                   
Sbjct: 997  SSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASF 1056

BLAST of Lag0032085 vs. NCBI nr
Match: XP_024035599.1 (uncharacterized protein LOC112096407 [Citrus clementina])

HSP 1 Score: 279.6 bits (714), Expect = 5.0e-71
Identity = 171/447 (38.26%), Postives = 234/447 (52.35%), Query Frame = 0

Query: 1   MNKKESIQPWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKY 60
           +N++ +I P NHT IV IPK+ +PR V++YRPI LCN+ Y  V K IANRLK  L+ I  
Sbjct: 384 LNQQGTIAPLNHTYIVLIPKNAKPRRVTEYRPISLCNVIYILVAKAIANRLKSELHKIIS 443

Query: 61  ECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLE 120
             Q AF+P R ITDN+IIG+E LH +++ +  K    ALKLD+ KAYDRVEW +L  V+E
Sbjct: 444 PVQSAFVPNRLITDNIIIGYECLHKIRHSKGKKNSSVALKLDIRKAYDRVEWSFLKHVIE 503

Query: 121 RLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTK----- 180
           RLGF    I+LIM+CI++ +FS+++NG A G I   RG+RQG PLS YLFLLC +     
Sbjct: 504 RLGFSSKWINLIMNCITTPTFSVVINGAANGLIHPQRGLRQGCPLSPYLFLLCAEEDKKG 563

Query: 181 ----------------GLSVMLQTTYS-CSISEQVWRVLENPNLTLLKLLKARYFPSSTL 240
                           GL     + ++   +++Q WR+L+NP   + K++KARY+ ++  
Sbjct: 564 IHWGKWEKLSYAKIRGGLGFRDVSCFNQALLAKQGWRLLQNPESLVAKVIKARYYKNTDF 623

Query: 241 FNTSAKSHSSYFWKGFLWGMDLLQKGTRRNLGNAK------------------------- 300
            N    S  S+ W+  LWG  +LQKGTR  +GN +                         
Sbjct: 624 LNAKVGSSPSFIWRSILWGRQVLQKGTRWRIGNGEKIQIQASNWIPRPTTFKLIVEPSLP 683

Query: 301 -------------------LQQHLCDKDVQLICSLPISQT-TPDLWTWHNDRTGSYSVRS 360
                              + Q     D  +I S+ + +T   D   WH DR G YSV+S
Sbjct: 684 TGAKVSELILPNQQWNESMINQSFARMDADIIKSILLPRTPQEDEIIWHYDRKGLYSVKS 743

Query: 361 GYKLSM---MNQQETSSAQPEE------ITNQTGP-KHFVWNSFHNSIPTMMNLWRHHIP 371
           GY+L++     +  TSSA   +        N  G  K FVW +    +PT  NLWR  I 
Sbjct: 744 GYQLALKIKFPEPPTSSAGASQEWQNLWKLNLPGKIKIFVWKAAKTFLPTAENLWRRKIL 803

BLAST of Lag0032085 vs. NCBI nr
Match: XP_030932623.1 (uncharacterized protein LOC115958352 [Quercus lobata])

HSP 1 Score: 255.0 bits (650), Expect = 1.3e-63
Identity = 161/491 (32.79%), Postives = 225/491 (45.82%), Query Frame = 0

Query: 11  NHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKYECQLAFIPGR 70
           N T I  IPK + P+ VSD+RPI LCN+ YK + KV+ANRLK  L     + Q AF+ GR
Sbjct: 464 NTTFITLIPKIKNPKKVSDFRPISLCNVIYKLIAKVVANRLKKFLAISVPDLQSAFLSGR 523

Query: 71  SITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLERLGFHEDLIH 130
            I+DN+++  ETLHYL+ K +GK G+ ALKLDMSK YDRVEW ++  +++ LG  E +  
Sbjct: 524 LISDNILVAFETLHYLKRKTRGKMGFMALKLDMSKTYDRVEWGFVEAIMQHLGLGERMRR 583

Query: 131 LIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVMLQ-------- 190
           +IM C+ S S+SIL+NG+ VGNIK SRG+RQGDPLS YLFLLC  GL  +L+        
Sbjct: 584 IIMSCMKSVSYSILLNGQPVGNIKPSRGLRQGDPLSPYLFLLCAMGLQGLLKKAEMEGRE 643

Query: 191 ----------TTYSCS-------------------------------------------- 250
                      TY+                                              
Sbjct: 644 VLIKAVIQAIPTYTMGCFKLPKGLIKELEVLIRKFWWGYNDDSRKVHWVSWERLCDAKEV 703

Query: 251 ---------------ISEQVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKG 310
                          +++QVWR+++NP     ++ KAR+FP+ ++ +    +  SY WK 
Sbjct: 704 GGMGFKEIEKFNDALLAKQVWRMMQNPESLCFRVFKARFFPNCSILDAKDSNTGSYAWKS 763

Query: 311 FLWGMDLLQKGTRRNLGNAKLQQHLCDK-------------------------------- 366
            L   D+++KG    +GN +  +   DK                                
Sbjct: 764 ILGAQDVIRKGMVWRIGNGQSVRIKEDKWLSVKPSRVTLSPLPSVMAETKVSSLINPELG 823

BLAST of Lag0032085 vs. NCBI nr
Match: XP_030925054.1 (uncharacterized protein LOC115952115 [Quercus lobata])

HSP 1 Score: 248.4 bits (633), Expect = 1.2e-61
Identity = 159/491 (32.38%), Postives = 223/491 (45.42%), Query Frame = 0

Query: 11   NHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKYECQLAFIPGR 70
            N T I  IPK + P+ VSD+RPI LCN+ YK + KV+ANRLK  L     + Q AF+ GR
Sbjct: 620  NTTFITLIPKIKNPKKVSDFRPISLCNVIYKLIAKVVANRLKKFLAISVPDSQSAFLSGR 679

Query: 71   SITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLERLGFHEDLIH 130
             I+DN+++  ETLHYL+ K +GK G+ ALKLDMSKAYDRVEW ++  +++ LG  E +  
Sbjct: 680  LISDNILVAFETLHYLKRKTRGKMGFMALKLDMSKAYDRVEWSFVEAIMQHLGLGERMRR 739

Query: 131  LIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVMLQ-------- 190
            +IM C+ S S+SIL+NG+ VGNIK SRG+RQG PLS YLFLLC  GL  +L+        
Sbjct: 740  IIMSCMKSVSYSILLNGQPVGNIKPSRGLRQGYPLSPYLFLLCAIGLQGLLKKAEMEGRE 799

Query: 191  ----------TTYSCS-------------------------------------------- 250
                       TY+                                              
Sbjct: 800  VLIKAVIQAIPTYTMGCFKLPKGLIKELEVLIRKFWWGYNDDSRKVHWVSWERLCEAKEV 859

Query: 251  ---------------ISEQVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKG 310
                           +++QVWR+++N      ++ KAR+FP+ ++ +    +  SY WK 
Sbjct: 860  GGMGFKEIEKFNDALLAKQVWRMMQNSESLCFRVFKARFFPNCSILDAKESNTGSYAWKS 919

Query: 311  FLWGMDLLQKGTRRNLGNAKLQQHLCDK-------------------------------- 366
             L   D+++KG    +GN +  +   DK                                
Sbjct: 920  ILGARDVIRKGMMWRIGNGQSVRIKEDKWLPVKSSRVILSPLPLVMAETKVSSLINLELG 979

BLAST of Lag0032085 vs. NCBI nr
Match: AAM93462.1 (putative reverse transcriptase [Oryza sativa Japonica Group])

HSP 1 Score: 240.0 bits (611), Expect = 4.4e-59
Identity = 139/404 (34.41%), Postives = 197/404 (48.76%), Query Frame = 0

Query: 10  WNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKYECQLAFIPG 69
           WN TNIV IPK + P ++ + RP  LCN+ YK ++KV+ANRLK IL +I  + Q AF+PG
Sbjct: 5   WNDTNIVLIPKVKSPSVLKELRPSSLCNVVYKIISKVLANRLKSILPEIILDTQSAFVPG 64

Query: 70  RSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLERLGFHEDLI 129
           R ITDN++I +E  H+LQNKR GK GYAA+KLDMSKAYDRVEWP+L  +L RLGF E   
Sbjct: 65  RLITDNVLIAYEATHFLQNKRNGKDGYAAIKLDMSKAYDRVEWPFLLHMLRRLGFDEKWN 124

Query: 130 HLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLS----------- 189
            LIM+C+++ ++ I VNG     IK  RG+RQGDPLS YLF++C +  S           
Sbjct: 125 RLIMNCVTTVNYKIKVNGDYTEVIKPDRGLRQGDPLSPYLFVICAEAFSAAIQAAEDDSL 184

Query: 190 VMLQTTYS--------------CS------------------------------------ 249
           ++L+ T S              CS                                    
Sbjct: 185 LLLKATESVANEMKQIILDYERCSGQIVNRDKSVVMFSSNMDEEEKKDKIMKPKSQGGLG 244

Query: 250 -----------ISEQVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKGFLWG 298
                      ++ Q WR+++NP+    +LLKA+YFP+  + +  ++   SY W+  L G
Sbjct: 245 FRDLHSFNIAMLARQGWRLIQNPDSLCSRLLKAKYFPNCNVLDAQSRKQMSYSWRSILKG 304

BLAST of Lag0032085 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 7.9e-19
Identity = 58/157 (36.94%), Postives = 87/157 (55.41%), Query Frame = 0

Query: 15  IVFIPK-SRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKYECQLAFIPGRSIT 74
           I  IPK  + P  + ++RPI L NI  K + K++ANR++  +  I +  Q+ FIPG    
Sbjct: 521 ITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQEHIKAIIHPDQVGFIPGMQGW 580

Query: 75  DNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLERLGFHEDLIHLIM 134
            N+      +HY+ NK K K  +  + LD  KA+D+++ P++ KVLER G     +++I 
Sbjct: 581 FNIRKSINVIHYI-NKLKDK-NHMIISLDAEKAFDKIQHPFMIKVLERSGIQGPYLNMIK 640

Query: 135 DCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLF 171
              S    +I VNG+ +  I    G RQG PLS YLF
Sbjct: 641 AIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLF 675

BLAST of Lag0032085 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 8.7e-18
Identity = 53/165 (32.12%), Postives = 85/165 (51.52%), Query Frame = 0

Query: 18  IPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKYECQLAFIPGRSITDNMI 77
           +PK    RL+ ++RP+ L +  YK V K I+ RLK +L ++ +  Q   +PGR+I DN+ 
Sbjct: 513 LPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLRLKSVLAEVIHPDQSYTVPGRTIFDNVF 572

Query: 78  IGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLERLGFHEDLIHLIMDCIS 137
           +  + LH+    R+     A L LD  KA+DRV+  YL   L+   F    +  +    +
Sbjct: 573 LIRDLLHF---ARRTGLSLAFLSLDQEKAFDRVDHQYLIGTLQAYSFGPQFVGYLKTMYA 632

Query: 138 SASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVMLQ 183
           SA   + +N      +   RG+RQG PLS  L+ L  +    +L+
Sbjct: 633 SAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLAIEPFLCLLR 674

BLAST of Lag0032085 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 1.2e-14
Identity = 59/224 (26.34%), Postives = 110/224 (49.11%), Query Frame = 0

Query: 3   KKESIQP--WNHTNIVFIPK-SRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIK 62
           +KE I P  +   +I+ IPK  R      ++RPI L NI  K + K++ANR++  +  + 
Sbjct: 500 EKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANRIQQHIKKLI 559

Query: 63  YECQLAFIPGRSITDNMIIGHETLHYLQNKRKGK-QGYAALKLDMSKAYDRVEWPYLSKV 122
           +  Q+ FIPG     N+    ++++ +Q+  + K + +  + +D  KA+D+++ P++ K 
Sbjct: 560 HHDQVGFIPGMQGWFNI---RKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQPFMLKT 619

Query: 123 LERLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLC----- 182
           L +LG     + +I       + +I++NG+ +       G RQG PLS  LF +      
Sbjct: 620 LNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNIVLEVLA 679

Query: 183 --------TKGLSVMLQTTYSCSISEQVWRVLENPNLTLLKLLK 210
                    KG+ +  +       ++ +   LENP ++   LLK
Sbjct: 680 RAIRQEKEIKGIQLGKEEVKLSLFADDMIVYLENPIVSAQNLLK 720

BLAST of Lag0032085 vs. ExPASy Swiss-Prot
Match: Q03274 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 74.7 bits (182), Expect = 3.2e-12
Identity = 49/177 (27.68%), Postives = 89/177 (50.28%), Query Frame = 0

Query: 9   PWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKYECQLAFIP 68
           PW       IPK       S++RPI + +   + + +++A RL+  +     +   A I 
Sbjct: 55  PWTAMRTTLIPKDGDLENPSNWRPITIASALQRLLHRILAKRLEAAVELHPAQKGYARID 114

Query: 69  GRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLERLGFHEDL 128
           G ++ +++++      Y+ ++R+ ++ Y  + LD+ KA+D V    + + L+RLG  E  
Sbjct: 115 G-TLVNSLLLD----TYISSRREQRKTYNVVSLDVRKAFDTVSHSSICRALQRLGIDEGT 174

Query: 129 IHLIMDCISSASFSILVN-GKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVMLQTT 185
            + I   +S ++ +I V  G     I   RG++QGDPLS +LF      L   LQ+T
Sbjct: 175 SNYITGSLSDSTTTIRVGPGSQTRKICIRRGVKQGDPLSPFLFNAVLDELLCSLQST 226

BLAST of Lag0032085 vs. ExPASy Swiss-Prot
Match: Q05118 (Retrovirus-related Pol polyprotein from type-1 retrotransposable element R2 (Fragment) OS=Popillia japonica OX=7064 PE=4 SV=1)

HSP 1 Score: 60.5 bits (145), Expect = 6.3e-08
Identity = 29/87 (33.33%), Postives = 47/87 (54.02%), Query Frame = 0

Query: 84  HYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLERLGFHEDLIHLIMDCISSASFSI 143
           HY++ +R   + Y  + LD+ KA+D V  P + + +   G  + +   IM  I+ A  +I
Sbjct: 11  HYIKLRRLKGKTYNVVSLDIRKAFDTVSHPAILRAMRAFGIDDGMQDFIMSTITDAYTNI 70

Query: 144 LVNGKAVGNIKSSRGIRQGDPLSSYLF 171
           +V G+    I    G++QGDPLS  LF
Sbjct: 71  VVGGRTTNKIYIRNGVKQGDPLSPVLF 97

BLAST of Lag0032085 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 4.9e-72
Identity = 191/638 (29.94%), Postives = 260/638 (40.75%), Query Frame = 0

Query: 1    MNKKESIQPWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKY 60
            +N  + I+ WN T I  IPK +QPR +SD+RPI LCN++YK ++K I NRLK ++  +  
Sbjct: 637  LNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLCNVSYKIISKSITNRLKNVIGLVIS 696

Query: 61   ECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLE 120
            + Q AF+P R+I+DN+IIGHE LH + + + G  G AALKLD+SKA+DRVEW YL  ++ 
Sbjct: 697  DAQSAFVPSRAISDNVIIGHECLHTINSCKSGLIGMAALKLDLSKAFDRVEWTYLECIMR 756

Query: 121  RLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVM 180
            ++GF+E  I  I+ CIS+  FSI +NG   G  + SRGIRQGDPLS YLFLLC +GLS +
Sbjct: 757  KMGFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGIRQGDPLSPYLFLLCAEGLSAL 816

Query: 181  L----------------------------------------------------------- 240
            +                                                           
Sbjct: 817  INHENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLESECLALRRLLDSYGRASGQCI 876

Query: 241  -----------------QTTYSC------------------------------------- 300
                             Q    C                                     
Sbjct: 877  NFSKSALLFSPNVHPERQQYLQCILNVKLVSHFGNYLGLPSHFTRRRGESRKLHWMKWGR 936

Query: 301  ---------------------SISEQVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSH 360
                                  +++ VWR L++PNL + K+LK +YF  ++L   S  S 
Sbjct: 937  MCYPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDTSLLQASNNSK 996

Query: 361  SSYFWKGFLWGMDLLQKGTRRNLGN----------------------------------- 403
            SSYFWKGFLWG DLL KG R  +GN                                   
Sbjct: 997  SSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNNGALDTTVASF 1056

BLAST of Lag0032085 vs. ExPASy TrEMBL
Match: A0A2N9GY38 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS35098 PE=3 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 4.0e-66
Identity = 180/577 (31.20%), Postives = 257/577 (44.54%), Query Frame = 0

Query: 1    MNKKESIQPWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKY 60
            +N  + ++  NHT I  IPK + P  V D+RPI LCN+ YK ++KV+ANRLKIIL  I  
Sbjct: 533  LNTGKILKAINHTYITLIPKIQNPEAVVDFRPISLCNVIYKIISKVLANRLKIILPKIVS 592

Query: 61   ECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLE 120
            E Q AF+PGR ITDN+++  ETLH++Q+++KG+ G  ALKLDMSKAYDRVEW YL +V+E
Sbjct: 593  ESQSAFVPGRLITDNILVAFETLHHMQHQKKGRIGSMALKLDMSKAYDRVEWKYLQRVME 652

Query: 121  RLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVM 180
            R+GF    + ++M+CIS+ S+SILVNG+    IK SR +RQGDPLS YLFLLC +G   +
Sbjct: 653  RMGFSSKWVTIMMECISTVSYSILVNGEPHRFIKPSRSLRQGDPLSPYLFLLCAEGFHSL 712

Query: 181  LQ---------------------------------------------TTYSCS------- 240
            LQ                                               Y+ S       
Sbjct: 713  LQKEKIAGALKGVSISRGGPKITHLFFADDSLLFCREILIKSMAQAIPAYAMSCFRLPNR 772

Query: 241  ----------------------------------------------------ISEQVWRV 300
                                                                +++QVWR+
Sbjct: 773  LIQEIEVLIRRFWWGQSGDKGKMHWLSWNTLCKSKASGGIGFRELGCFNEALLAKQVWRL 832

Query: 301  LENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKGFLWGMDLLQKGTRRNLGN----- 360
            + NP+    K+ KA+YFP  ++   +  S SSY WK  +   DL++KG+   +GN     
Sbjct: 833  MHNPSSLFYKVFKAKYFPRCSILEANPISTSSYAWKSIMSARDLIKKGSTWRVGNGSDIR 892

Query: 361  ----------------------AKLQQ--HLCD-----------------KDVQLICSLP 414
                                  + L Q  HL D                  D   I  +P
Sbjct: 893  IWGDKWLPSSYNKLISSPPLPQSSLSQVAHLIDHQSRTWKEGLIRETFLPHDATTIMGIP 952

BLAST of Lag0032085 vs. ExPASy TrEMBL
Match: A0A2N9F6W6 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS10717 PE=4 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 8.4e-64
Identity = 153/440 (34.77%), Postives = 220/440 (50.00%), Query Frame = 0

Query: 1    MNKKESIQPWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKY 60
            +N    ++  N T+I  IPK + P L+SDYRPI LCN+ YK ++KVIANRLK++L  I  
Sbjct: 908  LNSARILRKINLTHISLIPKKKNPELMSDYRPISLCNVVYKIISKVIANRLKVVLPLIIS 967

Query: 61   ECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLE 120
            + Q AF+PGR ITDN+ +  E +H  + KRKGK+G  ALKLDMSKAYDRVEW +L  +L 
Sbjct: 968  DPQSAFVPGRLITDNVSVAFEIIHKFKGKRKGKKGEMALKLDMSKAYDRVEWDFLEAILR 1027

Query: 121  RLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVM 180
            +LGF E  + +IM C+ S  + +L++    G I +SRGIRQGDPLS YLFL+C +GLS +
Sbjct: 1028 KLGFAEQWVAVIMSCVRSVQYLVLLDRVPKGYIITSRGIRQGDPLSPYLFLICAEGLSAL 1087

Query: 181  LQ-----------------------------------------------TTYSCS----- 240
            L+                                                 Y  S     
Sbjct: 1088 LRQASTSGRLKGTQTYRGGPWVSHLFFADDSLLFGQASIAESERFMAILNLYEASFRNLH 1147

Query: 241  ------ISEQVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKGFLWGMDLLQ 300
                  +++Q WR+L+NP     ++ KARYFPS ++   +  ++ S+ W+  L G ++LQ
Sbjct: 1148 MFNMALLAKQCWRLLQNPQSLFYRVFKARYFPSCSIMEANLGNNPSFLWQSILSGREVLQ 1207

Query: 301  KGTRRNLGNAKLQQHLCDKDVQLICSLPISQTTPDLWTWHNDRTGSYSVRSGYKLSMMNQ 360
            KG                    L    P +   P L  W   + GS++V+S Y L     
Sbjct: 1208 KG--------------------LSWVSPANGHAP-LPIWTRTKLGSFTVQSAYFLLESEN 1267

Query: 361  QETSSAQPEEITN-------------QTGPKHFVWNSFHNSIPTMMNLWRHHIPVEGICP 370
            + + S +   + +                 KHF+W ++H S+PT+ NL R  I     C 
Sbjct: 1268 RGSISGECSNVHSLRWFWKKTWKLAIPGKIKHFLWRAYHESLPTLFNLHRQKISSSPHCA 1326

BLAST of Lag0032085 vs. ExPASy TrEMBL
Match: A0A803PA46 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 1.6e-62
Identity = 184/643 (28.62%), Postives = 275/643 (42.77%), Query Frame = 0

Query: 1    MNKKESIQPWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKY 60
            +N+  +I P N T I  IPK +QP  V D+RPI LC I YK ++K IANRLK++LN +  
Sbjct: 592  LNENGNISPLNTTLITLIPKVKQPLSVKDFRPISLCTIIYKIISKTIANRLKLVLNKLIS 651

Query: 61   ECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLE 120
              Q AF+PGR I+DN+II  E  H ++ K +G++G+ ALKLDM+KA+DRVEW +LS +L+
Sbjct: 652  SNQSAFLPGRLISDNIIIAQEVAHTIKLKSQGRKGWMALKLDMAKAFDRVEWAFLSAILK 711

Query: 121  RLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVM 180
            ++ F      LI+DCI++A+F + VNGK  G+I  +RGIRQGDPLS YLFLLC +GLS +
Sbjct: 712  KMDFPPRFSRLIVDCITTATFKMNVNGKVFGHITPTRGIRQGDPLSPYLFLLCAEGLSSI 771

Query: 181  LQ-----------------------------------TTYSCS----------------- 240
            ++                                   +  SC+                 
Sbjct: 772  IRKFERTGDLSGFKVARRAPPISHLLFADDSFLFCRASITSCNAIKEVLSLQSPPTQWHA 831

Query: 241  ---------------------------------------------------------ISE 300
                                                                     +++
Sbjct: 832  LASLSTWLLESIMANFWWGFNENNRPKIHWQSWPKLCKSKKHGGLGFRSLVHFNQALLAK 891

Query: 301  QVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKGFLWGMDLLQKGTRRNLGN 360
            Q WR+L++PN  + ++L ARY P+S+    S     S+ W+   WG +LL KG    +GN
Sbjct: 892  QAWRILKHPNSMVAQILAARYHPNSSFMTCSLGHSPSFVWRSIFWGKELLHKGLISKIGN 951

Query: 361  AK----------------------------------------LQQHLCDKDVQLICSLPI 420
             +                                        LQ       VQ I S+P+
Sbjct: 952  GQNTSTTLDNWIPGHNRVTPIGPIPDRVSSFISPNMTWDLPSLQSCFPSYIVQEILSIPL 1011

Query: 421  S-QTTPDLWTWHNDRTGSYSVRSGYKLSMMNQQETSSAQPEEITNQTGP----------- 460
                  D   W   ++G+YSV+SGY LS       SS  P +I + + P           
Sbjct: 1012 PLSPMNDNLIWKLTKSGTYSVKSGYHLSF------SSTSPPDIPSPSSPSPWWKNLWLLH 1071

BLAST of Lag0032085 vs. ExPASy TrEMBL
Match: A0A2N9FR17 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS17540 PE=4 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 2.1e-62
Identity = 150/425 (35.29%), Postives = 216/425 (50.82%), Query Frame = 0

Query: 1    MNKKESIQPWNHTNIVFIPKSRQPRLVSDYRPIRLCNIAYKTVTKVIANRLKIILNDIKY 60
            +N    +   NHT I  IPK++ P  V+++RPI LCN+ YK ++KV+ANRLKIIL  I  
Sbjct: 811  LNSGRILNSINHTFITLIPKTKNPERVTEFRPISLCNVIYKLISKVLANRLKIILPQIVS 870

Query: 61   ECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKAYDRVEWPYLSKVLE 120
            + Q AF+ GR ITDN+++  ETLHY+ + + G+ G  ALKLDM+KAYDRVEW +L K++ 
Sbjct: 871  DSQSAFVLGRLITDNVLVAFETLHYMHHNKIGRDGAMALKLDMNKAYDRVEWIFLEKIMS 930

Query: 121  RLGFHEDLIHLIMDCISSASFSILVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLSVM 180
            R+GFH+  I L+ +CIS+ S+SILVNG+  G IK SRG+RQGDPLS YLFLLC +GL  +
Sbjct: 931  RMGFHQKWISLMTECISTVSYSILVNGEPHGYIKPSRGLRQGDPLSPYLFLLCAEGLHSL 990

Query: 181  LQTT-----------------------------------------------YSCSISEQV 240
            +                                                  Y  +  +QV
Sbjct: 991  INKAGRDGDIQGVSLCRKGPKITHLFFADDNLLFSKATPTACEKIQGILDQYEKASGQQV 1050

Query: 241  WR----------VLENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKGFLWGMDLLQK 300
             R            E     + + L+A++FP  T+ N    +  SY W+  L   +L+ K
Sbjct: 1051 NRDKTTIYFSKNTPEASQNVIKEALEAKFFPHGTILNCPTTTPGSYAWQSLLKARELITK 1110

Query: 301  GTRRNLGNAKLQQHLCDKDVQLICSLPIS-QTTPDLWTWHNDRTGSYSVRSGYKLSMMNQ 360
              +    ++ +       DV+ I  +P+S Q   D   W  +  G YSVRSGY+  +   
Sbjct: 1111 -PQMTWDHSLIDSLFLPYDVEAIKQIPLSNQDHADKLIWPGNTHGDYSVRSGYRFLV--- 1170

Query: 361  QETSSAQPEEITNQTGPKHFVWNSFHNSIPTMMNLWRHHIPVEGICPIFKFNPETTEHAL 368
                    EE  N  G       +   ++PT +NL + HIP+   C +     E T HAL
Sbjct: 1171 -------DEEDKNLPGSS----RATREALPTKLNLQKRHIPIGTTCELCGKKDEDTIHAL 1220

BLAST of Lag0032085 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 70.5 bits (171), Expect = 4.3e-12
Identity = 34/80 (42.50%), Postives = 49/80 (61.25%), Query Frame = 0

Query: 47  IANRLKIILNDIKYECQLAFIPGRSITDNMIIGHETLHYLQNKRKGKQGYAALKLDMSKA 106
           +  RLK ++ ++    Q +FIPGR  TDN++   E +H ++ K KG +G+  LKLD+ KA
Sbjct: 1   MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRK-KGVKGWMLLKLDLEKA 60

Query: 107 YDRVEWPYLSKVLERLGFHE 127
           YDR+ W YL   L   GF E
Sbjct: 61  YDRIRWDYLEDTLISAGFPE 79

BLAST of Lag0032085 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 53.1 bits (126), Expect = 7.1e-07
Identity = 21/63 (33.33%), Postives = 40/63 (63.49%), Query Frame = 0

Query: 189 ISEQVWRVLENPNLTLLKLLKARYFPSSTLFNTSAKSHSSYFWKGFLWGMDLLQKGTRRN 248
           +++Q +R++  P+  L +LL++RYFP S++   S  +  SY W+  + G +LL +G  R 
Sbjct: 70  LAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRT 129

Query: 249 LGN 252
           +G+
Sbjct: 130 IGD 132

BLAST of Lag0032085 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 48.1 bits (113), Expect = 2.3e-05
Identity = 21/35 (60.00%), Postives = 27/35 (77.14%), Query Frame = 0

Query: 144 LVNGKAVGNIKSSRGIRQGDPLSSYLFLLCTKGLS 179
           ++NG   G +  SRG+RQGDPLS YLF+LCT+ LS
Sbjct: 13  IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLS 47