Lag0032079 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0032079
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	tropinone reductase-like 1
Location	chr11: 23850165 .. 23852103 (+)
RNA-Seq Expression	Lag0032079
Synteny	Lag0032079

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAATCTTAATGCCTCTGCAGCTTCTCTTAGAAGGTAAGGTTCCATGGTGATAAACCAATTTGAAAACCCTTTTTCTCTTTAATTAATAAATAGCCAACTATATGTTAATATTTGTTTTCTTTTTAATGTTTGGATTTTTTTTCTTCATATATATATATAAAGTCTCTATTGTTTGAAGAATTTTTTTTTTTTCCCAAAAATGAAAAGAGGTATTTTCTCTCTTTTATCCATCAGAAATTTAGGAGGACTACTCAGTCGATAGAGAAATGTTTATTCTATCATCATTCCTAGAAATATATTTTTCATAAATGAAAATTAAATATGTTTTTTTTAATAGTAAATGACAATATTTTATGAGTATAGAGTCATTCCTAAGAATTTTGCAGAAATCTCAAACTTAAATGGTTCCATTTCTTCTCCAAATTGGTATTATAAATAATTGATAGTTAAAACGAAAAGAAAAAGGAAAAGAAAAGTGTATATATATATATTTATATATAAATTAAAATAAAGCCATTTAATTAATATGTGTAATTTGAGAGTTTTCAGATTGGTGGGGAAGGTGGCGATTATCACGGGCGGCGCAAGCGGGATCGGAGCTAGCGCAGTGCATAGATTTCATGAAAACGGAGCGAAAGTGGTAATCGCCGATATCCAAGACGAAGTCGGCCAAAAAATCGCCGACGAGATCGGGAAAGACGTTAGCTACGTGCACTGCGATGTGTCAAAGGAAGATGACGTCAGAAATCTGGTGGACACCGCCGTCAGCCGGCACGGCAAGCTCGACATCATGTACTGCAATGCCGGCGTCATCGACCGGCCGTTTGGCGGCATATTGGATGTTACAAAATCTGACGTGGACAAGGTTTTATTAAATTTAATTTATCCCTTCGCCCAAAAATAATAATGCCTTTAATTCTATTTTCATCCTATATTTTTAAGATTGTCTTTTTTTTTCCTCTTTTTCCAGCTTTTCCAAATATTTTTATTCCGTGACTCTAAAAAATAAATAACAAACAGACAATACTTACTGAGTCAACAACGGTTGAGTTTTTAACAAATGAGAGAGAGAAATGAAAAAAAAAAAAGTTTTGATGGGTAGTGGTAAACATTATTCTTGATGAATTAAGAAGCAAACATTGCTATTTAAAAATAATCCTTTTTTATTTTCACATTAAATTAAGAAGCAAACATTATTCTTAATTTCATAGGTTATAAGTGAGTCTTGAGTAAGTAAATATTATGTTAATTAAAATATAATATAATAAACATTGTTTTAAGAATCTGTATTAAAGAGTAGACAAATATTGTAAATGCAATTAAATATCTTCTCTAAACTGAAATTTACTTGATGGAGGATACATGTCAATTACCTTTGCTATGTACTCGTTTTGACAATGTTTTTTTTTTTTTTTTTGAATACTTAGAAAGTCATTTCAAACAAGCATTTAATAATGTATTCTCAGGTATTGGGTGTGAACGTGATGGGAGCATTTTGGGGGGCGAAGCAGGCAGCGAGGGTTATGATACCGGAGAAAAAAGGGTGCATTCTATTCACAACCAGCGTAGCGGCAAGCATCGCAGGGCTGTCGACGCACCCATACGCGGCGTCGAAGTGCGCCGTGTTGGGGTTGGTGAGAAACCTGGCGGCAGAACTGGGGCAGCACGGGATCCGAGTCAACTGTGTGGCGCCATTTGTGGTGGCGACGCCAATTACTGGGCTAAGCGACTCGGCGCAAGTGGAGGCGATGGAGACCATGGTGCGCGGATGGGCGAATCTCAAGGGTCAATCCCTCAAGGCCGACGACATAGCGAATGCGGCTCTGTACTTGGCTAGCGATGAGGCCGACTATGTCAGTGGCCTTAATCTCATCGTCGATGGTGGCTATAGTGTCGTCAATCCTTCTATGCTTAACAGTCTTAAGCTCATGGAGTGA

mRNA sequence

ATGAATCTTAATGCCTCTGCAGCTTCTCTTAGAAGATTGGTGGGGAAGGTGGCGATTATCACGGGCGGCGCAAGCGGGATCGGAGCTAGCGCAGTGCATAGATTTCATGAAAACGGAGCGAAAGTGGTAATCGCCGATATCCAAGACGAAGTCGGCCAAAAAATCGCCGACGAGATCGGGAAAGACGTTAGCTACGTGCACTGCGATGTGTCAAAGGAAGATGACGTCAGAAATCTGGTGGACACCGCCGTCAGCCGGCACGGCAAGCTCGACATCATGTACTGCAATGCCGGCGTCATCGACCGGCCGTTTGGCGGCATATTGGATGTTACAAAATCTGACGTGGACAAGGTATTGGGTGTGAACGTGATGGGAGCATTTTGGGGGGCGAAGCAGGCAGCGAGGGTTATGATACCGGAGAAAAAAGGGTGCATTCTATTCACAACCAGCGTAGCGGCAAGCATCGCAGGGCTGTCGACGCACCCATACGCGGCGTCGAAGTGCGCCGTGTTGGGGTTGGTGAGAAACCTGGCGGCAGAACTGGGGCAGCACGGGATCCGAGTCAACTGTGTGGCGCCATTTGTGGTGGCGACGCCAATTACTGGGCTAAGCGACTCGGCGCAAGTGGAGGCGATGGAGACCATGGTGCGCGGATGGGCGAATCTCAAGGGTCAATCCCTCAAGGCCGACGACATAGCGAATGCGGCTCTGTACTTGGCTAGCGATGAGGCCGACTATGTCAGTGGCCTTAATCTCATCGTCGATGGTGGCTATAGTGTCGTCAATCCTTCTATGCTTAACAGTCTTAAGCTCATGGAGTGA

Coding sequence (CDS)

ATGAATCTTAATGCCTCTGCAGCTTCTCTTAGAAGATTGGTGGGGAAGGTGGCGATTATCACGGGCGGCGCAAGCGGGATCGGAGCTAGCGCAGTGCATAGATTTCATGAAAACGGAGCGAAAGTGGTAATCGCCGATATCCAAGACGAAGTCGGCCAAAAAATCGCCGACGAGATCGGGAAAGACGTTAGCTACGTGCACTGCGATGTGTCAAAGGAAGATGACGTCAGAAATCTGGTGGACACCGCCGTCAGCCGGCACGGCAAGCTCGACATCATGTACTGCAATGCCGGCGTCATCGACCGGCCGTTTGGCGGCATATTGGATGTTACAAAATCTGACGTGGACAAGGTATTGGGTGTGAACGTGATGGGAGCATTTTGGGGGGCGAAGCAGGCAGCGAGGGTTATGATACCGGAGAAAAAAGGGTGCATTCTATTCACAACCAGCGTAGCGGCAAGCATCGCAGGGCTGTCGACGCACCCATACGCGGCGTCGAAGTGCGCCGTGTTGGGGTTGGTGAGAAACCTGGCGGCAGAACTGGGGCAGCACGGGATCCGAGTCAACTGTGTGGCGCCATTTGTGGTGGCGACGCCAATTACTGGGCTAAGCGACTCGGCGCAAGTGGAGGCGATGGAGACCATGGTGCGCGGATGGGCGAATCTCAAGGGTCAATCCCTCAAGGCCGACGACATAGCGAATGCGGCTCTGTACTTGGCTAGCGATGAGGCCGACTATGTCAGTGGCCTTAATCTCATCGTCGATGGTGGCTATAGTGTCGTCAATCCTTCTATGCTTAACAGTCTTAAGCTCATGGAGTGA

Protein sequence

MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGVNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAELGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLASDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME

Homology

[Close]

BLAST of Lag0032079 vs. NCBI nr
Match: XP_022934668.1 (tropinone reductase-like 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 450.3 bits (1157), Expect = 1.2e-122
Identity = 228/273 (83.52%), Postives = 246/273 (90.11%), Query Frame = 0


Query: 1   MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIG 60
           M+ NASA +LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDEVGQKIAD++G
Sbjct: 1   MSFNASATTLRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEVGQKIADQLG 60

Query: 61  KDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 120
           +D+SY+HCDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVLG
Sbjct: 61  EDISYIHCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVLG 120

Query: 121 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 180
           VNVMGAFWGAK AARVMIP+KKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAAE
Sbjct: 121 VNVMGAFWGAKHAARVMIPDKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAAE 180

Query: 181 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 240
           LGQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYLA
Sbjct: 181 LGQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYLA 240

Query: 241 SDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           SDEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 241 SDEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 273

[Close]

BLAST of Lag0032079 vs. NCBI nr
Match: XP_023527968.1 (tropinone reductase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 448.0 bits (1151), Expect = 5.9e-122
Identity = 229/273 (83.88%), Postives = 245/273 (89.74%), Query Frame = 0


Query: 1   MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIG 60
           M+ NASA +LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDEVGQKIAD++G
Sbjct: 1   MSFNASATALRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEVGQKIADQLG 60

Query: 61  KDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 120
           +DVSY+HCDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVLG
Sbjct: 61  EDVSYIHCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVLG 120

Query: 121 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 180
           VNVMGAFWGAK AARVMI EKKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAAE
Sbjct: 121 VNVMGAFWGAKHAARVMILEKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAAE 180

Query: 181 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 240
           LGQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYLA
Sbjct: 181 LGQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYLA 240

Query: 241 SDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           SDEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 241 SDEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 273

[Close]

BLAST of Lag0032079 vs. NCBI nr
Match: KAG6580722.1 (hypothetical protein SDJN03_20724, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 448.0 bits (1151), Expect = 5.9e-122
Identity = 227/272 (83.46%), Postives = 245/272 (90.07%), Query Frame = 0


Query: 2   NLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGK 61
           N +ASA +LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDEVGQKIAD++G+
Sbjct: 4   NASASATALRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEVGQKIADQLGE 63

Query: 62  DVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGV 121
           D+SY+HCDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVLGV
Sbjct: 64  DISYIHCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVLGV 123

Query: 122 NVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAEL 181
           NVMGAFWGAK AARVMIP+KKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAAEL
Sbjct: 124 NVMGAFWGAKHAARVMIPDKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAAEL 183

Query: 182 GQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLAS 241
           GQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYLAS
Sbjct: 184 GQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYLAS 243

Query: 242 DEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           DEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 244 DEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 275

[Close]

BLAST of Lag0032079 vs. NCBI nr
Match: XP_022983067.1 (tropinone reductase-like 1 [Cucurbita maxima])

HSP 1 Score: 446.8 bits (1148), Expect = 1.3e-121
Identity = 227/273 (83.15%), Postives = 244/273 (89.38%), Query Frame = 0


Query: 1   MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIG 60
           M+ NASA +LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDE GQKIAD++G
Sbjct: 1   MSFNASATALRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEAGQKIADQLG 60

Query: 61  KDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 120
           +DVSY+ CDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVLG
Sbjct: 61  EDVSYIRCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVLG 120

Query: 121 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 180
           VNVMGAFWGAK AARVM+PEKKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAAE
Sbjct: 121 VNVMGAFWGAKHAARVMVPEKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAAE 180

Query: 181 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 240
           LGQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYLA
Sbjct: 181 LGQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYLA 240

Query: 241 SDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           SDEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 241 SDEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 273

[Close]

BLAST of Lag0032079 vs. NCBI nr
Match: XP_022934667.1 (tropinone reductase-like 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 446.0 bits (1146), Expect = 2.3e-121
Identity = 229/274 (83.58%), Postives = 246/274 (89.78%), Query Frame = 0


Query: 1   MNLNASAAS-LRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEI 60
           M  NASAA+ LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDEVGQKIAD++
Sbjct: 1   MTSNASAATPLRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEVGQKIADQL 60

Query: 61  GKDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVL 120
           G+D+SY+HCDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVL
Sbjct: 61  GEDISYIHCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVL 120

Query: 121 GVNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAA 180
           GVNVMGAFWGAK AARVMIP+KKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAA
Sbjct: 121 GVNVMGAFWGAKHAARVMIPDKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAA 180

Query: 181 ELGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYL 240
           ELGQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYL
Sbjct: 181 ELGQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYL 240

Query: 241 ASDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           ASDEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 241 ASDEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 274

[Close]

BLAST of Lag0032079 vs. ExPASy Swiss-Prot
Match: H9BFQ0 (Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 297.7 bits (761), Expect = 1.3e-79
Identity = 154/269 (57.25%), Postives = 204/269 (75.84%), Query Frame = 0


Query: 3   LNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD 62
           + + A   +RL GKVAIITGGASGIGA     FHENGAKVVIADIQD++GQ +A ++G  
Sbjct: 1   MTSIAGPHKRLEGKVAIITGGASGIGACTAELFHENGAKVVIADIQDDLGQALATKLGGK 60

Query: 63  VSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPF--GGILDVTKSDVDKVLG 122
             Y+HCDVSKEDDV NLVDT V+++G+LDIM+ NAG+I+       +++  KSD+D++L 
Sbjct: 61  ACYIHCDVSKEDDVINLVDTTVAKYGRLDIMFNNAGIIEGQGLPVSVVESEKSDLDRLLS 120

Query: 123 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 182
           VN+ GAF GAK A RVM+ ++KGCILFT+S+  SIAGLS H YAASK  V GL +NL  E
Sbjct: 121 VNLGGAFLGAKHATRVMVQQRKGCILFTSSLCTSIAGLSGHAYAASKSGVCGLAKNLTPE 180

Query: 183 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 242
           LG++GIRVNC++P+ + T I+ +S+ A  E +E M+     L GQ+L+AD IA AAL+LA
Sbjct: 181 LGKYGIRVNCISPYGLVTGISNISE-ANRELVEAMLSELGTLSGQTLRADGIAKAALFLA 240

Query: 243 SDEADYVSGLNLIVDGGYSVVNPSMLNSL 270
           SDEA YVSG+N++VDGGYSVVNP + +++
Sbjct: 241 SDEAYYVSGINMVVDGGYSVVNPRLADAI 268

[Close]

BLAST of Lag0032079 vs. ExPASy Swiss-Prot
Match: H9BFQ1 (Tropinone reductase-like 2 OS=Erythroxylum coca OX=289672 PE=2 SV=1)

HSP 1 Score: 297.0 bits (759), Expect = 2.2e-79
Identity = 153/271 (56.46%), Postives = 204/271 (75.28%), Query Frame = 0


Query: 3   LNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD 62
           + + A   +RL GKVAIITGGASGIGA     FHENGAKVVIADIQD++GQ +A ++G  
Sbjct: 1   MTSVAGPHKRLEGKVAIITGGASGIGACTAELFHENGAKVVIADIQDDLGQALATKLGGK 60

Query: 63  VSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPF--GGILDVTKSDVDKVLG 122
             Y+HCDVSKED+V NLVDT V+++G+LDIM+ NAG+I+       +++  KSD+D++L 
Sbjct: 61  ACYIHCDVSKEDEVINLVDTTVAKYGRLDIMFNNAGIIEGQGLPVSVVESEKSDLDRLLS 120

Query: 123 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 182
           VN+ GAF GAK A RVM+ ++KGCILFT+SV  SIAGLS H YAASK  V GL +NL  E
Sbjct: 121 VNLGGAFLGAKHATRVMVQQRKGCILFTSSVCTSIAGLSGHAYAASKSGVCGLAKNLTPE 180

Query: 183 LGQHGIRVNCVAPFVVATPITGLSDSAQV--EAMETMVRGWANLKGQSLKADDIANAALY 242
           LG++GIRVNC++P+ + T ++ +S   +   E +E M+     L GQ+L+AD IA AAL+
Sbjct: 181 LGKYGIRVNCISPYGLVTGVSNVSGEGEANREFVEAMLSELGTLSGQTLRADGIAKAALF 240

Query: 243 LASDEADYVSGLNLIVDGGYSVVNPSMLNSL 270
           LASDEA YVSG+N++VDGGYSVVNP +++S+
Sbjct: 241 LASDEAYYVSGINMVVDGGYSVVNPRLVDSI 271

[Close]

BLAST of Lag0032079 vs. ExPASy Swiss-Prot
Match: A3F5F0 (Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH PE=1 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.2e-69
Identity = 145/269 (53.90%), Postives = 190/269 (70.63%), Query Frame = 0


Query: 7   AASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD--VS 66
           A+S  RL  KVAIITGGA GIG +    F   GAKVVIADI D+ GQK+ + IG    +S
Sbjct: 8   ASSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADISDDHGQKVCNNIGSPDVIS 67

Query: 67  YVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGVNVM 126
           +VHCDV+K++DVRNLVDT +++HGKLDIM+ N GV+      IL+    D  +V+ +NV 
Sbjct: 68  FVHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVY 127

Query: 127 GAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLS-THPYAASKCAVLGLVRNLAAELGQ 186
           GAF  AK AARVMIP KKG I+FT S+++  AG   +H Y A+K AVLGL  +L  ELGQ
Sbjct: 128 GAFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGQ 187

Query: 187 HGIRVNCVAPFVVATPITGLSDSAQVEA--METMVRGWANLKGQSLKADDIANAALYLAS 246
           HGIRVNCV+P+VVA+P+  L+D   V++  +E +    ANLKG  L+A+D+A+A  YLA 
Sbjct: 188 HGIRVNCVSPYVVASPL--LTDVFGVDSSRVEELAHQAANLKGILLRAEDVADAVAYLAG 247

Query: 247 DEADYVSGLNLIVDGGYSVVNPSMLNSLK 271
           DE+ YVSGLNL++DGGY+  NP+   +LK
Sbjct: 248 DESKYVSGLNLVIDGGYTRTNPAFPTALK 274

[Close]

BLAST of Lag0032079 vs. ExPASy Swiss-Prot
Match: Q7FAE1 (Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 7.9e-69
Identity = 142/260 (54.62%), Postives = 184/260 (70.77%), Query Frame = 0


Query: 7   AASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKDV-SY 66
           +A  R+LVGKVA+ITGGASGIGA     F ++GA+VV+ADIQDE+G  +  E+G D  SY
Sbjct: 9   SADARKLVGKVAVITGGASGIGACTARLFVKHGARVVVADIQDELGASLVAELGPDASSY 68

Query: 67  VHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGVNVMG 126
           VHCDV+ E DV   VD AV+R GKLD+M+ NAGV   P   + + TK D ++VL VN++G
Sbjct: 69  VHCDVTNEGDVAAAVDHAVARFGKLDVMFNNAGVSGPPCFRMSECTKEDFERVLAVNLVG 128

Query: 127 AFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAELGQHG 186
            F G K AARVM P ++G I+ T S+++S++G ++H Y  SK A++G   N A ELG+HG
Sbjct: 129 PFLGTKHAARVMAPARRGSIISTASLSSSVSGAASHAYTTSKHALVGFTENAAGELGRHG 188

Query: 187 IRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQ-SLKADDIANAALYLASDEA 246
           IRVNCV+P  VATP+   +     EA+E ++   ANLKG  +LKADDIA AAL+LASD+ 
Sbjct: 189 IRVNCVSPAGVATPLARAAMGMDDEAIEAIMANSANLKGAGALKADDIAAAALFLASDDG 248

Query: 247 DYVSGLNLIVDGGYSVVNPS 265
            YVSG NL VDGG SVVN S
Sbjct: 249 RYVSGQNLRVDGGLSVVNSS 268

[Close]

BLAST of Lag0032079 vs. ExPASy Swiss-Prot
Match: Q94KL8 (Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 PE=1 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 3.9e-68
Identity = 141/268 (52.61%), Postives = 189/268 (70.52%), Query Frame = 0


Query: 8   ASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD--VSY 67
           +S  RL  KVAIITGGA GIG +    F   GAKVVIADI D+ GQK+ + IG    +S+
Sbjct: 9   SSTNRLQDKVAIITGGAGGIGETTAKLFVRYGAKVVIADIADDHGQKVCNNIGSPDVISF 68

Query: 68  VHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGVNVMG 127
           VHCDV+K++DVRNLVDT +++HGKLDIM+ N GV+      IL+    D  +V+ +NV G
Sbjct: 69  VHCDVTKDEDVRNLVDTTIAKHGKLDIMFGNVGVLSTTPYSILEAGNEDFKRVMDINVYG 128

Query: 128 AFWGAKQAARVMIPEKKGCILFTTSVAASIAGLS-THPYAASKCAVLGLVRNLAAELGQH 187
           AF  AK AARVMIP KKG I+FT S+++  AG   +H Y A+K AVLGL  +L  ELG++
Sbjct: 129 AFLVAKHAARVMIPAKKGSIVFTASISSFTAGEGVSHVYTATKHAVLGLTTSLCTELGEY 188

Query: 188 GIRVNCVAPFVVATPITGLSDSAQVEA--METMVRGWANLKGQSLKADDIANAALYLASD 247
           GIRVNCV+P++VA+P+  L+D   V++  +E +    ANLKG  L+A+D+A+A  YLA D
Sbjct: 189 GIRVNCVSPYIVASPL--LTDVFGVDSSRVEELAHQAANLKGTLLRAEDVADAVAYLAGD 248

Query: 248 EADYVSGLNLIVDGGYSVVNPSMLNSLK 271
           E+ YVSGLNL++DGGY+  NP+   +LK
Sbjct: 249 ESKYVSGLNLVIDGGYTRTNPAFPTALK 274

[Close]

BLAST of Lag0032079 vs. ExPASy TrEMBL
Match: A0A6J1F8A8 (tropinone reductase-like 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441791 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 5.8e-123
Identity = 228/273 (83.52%), Postives = 246/273 (90.11%), Query Frame = 0


Query: 1   MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIG 60
           M+ NASA +LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDEVGQKIAD++G
Sbjct: 1   MSFNASATTLRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEVGQKIADQLG 60

Query: 61  KDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 120
           +D+SY+HCDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVLG
Sbjct: 61  EDISYIHCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVLG 120

Query: 121 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 180
           VNVMGAFWGAK AARVMIP+KKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAAE
Sbjct: 121 VNVMGAFWGAKHAARVMIPDKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAAE 180

Query: 181 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 240
           LGQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYLA
Sbjct: 181 LGQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYLA 240

Query: 241 SDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           SDEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 241 SDEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 273

[Close]

BLAST of Lag0032079 vs. ExPASy TrEMBL
Match: A0A6J1IY89 (tropinone reductase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111481723 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 6.4e-122
Identity = 227/273 (83.15%), Postives = 244/273 (89.38%), Query Frame = 0


Query: 1   MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIG 60
           M+ NASA +LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDE GQKIAD++G
Sbjct: 1   MSFNASATALRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEAGQKIADQLG 60

Query: 61  KDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 120
           +DVSY+ CDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVLG
Sbjct: 61  EDVSYIRCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVLG 120

Query: 121 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 180
           VNVMGAFWGAK AARVM+PEKKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAAE
Sbjct: 121 VNVMGAFWGAKHAARVMVPEKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAAE 180

Query: 181 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 240
           LGQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYLA
Sbjct: 181 LGQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYLA 240

Query: 241 SDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           SDEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 241 SDEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 273

[Close]

BLAST of Lag0032079 vs. ExPASy TrEMBL
Match: A0A6J1F3G3 (tropinone reductase-like 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441791 PE=4 SV=1)

HSP 1 Score: 446.0 bits (1146), Expect = 1.1e-121
Identity = 229/274 (83.58%), Postives = 246/274 (89.78%), Query Frame = 0


Query: 1   MNLNASAAS-LRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEI 60
           M  NASAA+ LRRL GKVAIITGGASGIGAS V  FHENGAKV+IADIQDEVGQKIAD++
Sbjct: 1   MTSNASAATPLRRLEGKVAIITGGASGIGASVVRIFHENGAKVIIADIQDEVGQKIADQL 60

Query: 61  GKDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVL 120
           G+D+SY+HCDVSKE+DV NLVD AV RHGKLDIMY NAGVIDRPF GILDVTKSD+DKVL
Sbjct: 61  GEDISYIHCDVSKEEDVSNLVDAAVCRHGKLDIMYSNAGVIDRPFSGILDVTKSDLDKVL 120

Query: 121 GVNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAA 180
           GVNVMGAFWGAK AARVMIP+KKGCILFTTS   +IAGLSTHPYAASKCAVLGLVRNLAA
Sbjct: 121 GVNVMGAFWGAKHAARVMIPDKKGCILFTTSATTNIAGLSTHPYAASKCAVLGLVRNLAA 180

Query: 181 ELGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYL 240
           ELGQHGIRVNCVAPFVVAT I G +D  Q EA+E MV GWANLKG  LKADDIA AALYL
Sbjct: 181 ELGQHGIRVNCVAPFVVATGIAGPTDPKQAEALENMVTGWANLKGCVLKADDIARAALYL 240

Query: 241 ASDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           ASDEA+YVSGLNL+VDGGYSVVNPSML +LKLM+
Sbjct: 241 ASDEANYVSGLNLVVDGGYSVVNPSMLKTLKLMD 274

[Close]

BLAST of Lag0032079 vs. ExPASy TrEMBL
Match: Q9SBM0 (Wts2L OS=Citrullus lanatus OX=3654 GN=wts2L PE=2 SV=1)

HSP 1 Score: 438.3 bits (1126), Expect = 2.3e-119
Identity = 223/273 (81.68%), Postives = 240/273 (87.91%), Query Frame = 0


Query: 1   MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIG 60
           M LN S   LRRL GKVAIITGGASGIG SAV  FHENGAKV+IADIQDE+GQKIADE+G
Sbjct: 1   MTLNRSPTPLRRLDGKVAIITGGASGIGTSAVRIFHENGAKVIIADIQDEIGQKIADELG 60

Query: 61  KDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 120
            DVSY+HCDVSKEDDV NLVD AV RHGKLDIMY NAGV+DR F GILDVTKSD+DKVLG
Sbjct: 61  DDVSYIHCDVSKEDDVSNLVDAAVHRHGKLDIMYSNAGVLDRSFSGILDVTKSDLDKVLG 120

Query: 121 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 180
           VNVMGAFWGAK AARVMIPEK GCILFT+S   +IAGLS+HPYAASKCAVLGLVRNLAAE
Sbjct: 121 VNVMGAFWGAKHAARVMIPEKNGCILFTSSATTNIAGLSSHPYAASKCAVLGLVRNLAAE 180

Query: 181 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 240
           LGQHGIRVNCVAPFVVAT I G  D  Q EA+ETMV  WANLKG+ LKADDIA AALYLA
Sbjct: 181 LGQHGIRVNCVAPFVVATGIAGSRDPMQAEALETMVTTWANLKGRVLKADDIAKAALYLA 240

Query: 241 SDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           SD+A+YVSGLNL+VDGGYSVVNP+ML +LKLM+
Sbjct: 241 SDDANYVSGLNLVVDGGYSVVNPTMLKTLKLMD 273

[Close]

BLAST of Lag0032079 vs. ExPASy TrEMBL
Match: A0A5D3DNU4 (Tropinone reductase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G004270 PE=4 SV=1)

HSP 1 Score: 429.1 bits (1102), Expect = 1.4e-116
Identity = 219/273 (80.22%), Postives = 238/273 (87.18%), Query Frame = 0


Query: 1   MNLNASAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIG 60
           M LN S   LRRL GKVAIITGGASGIGASAV  FHENGAKV IADIQDE GQKI+DE+G
Sbjct: 1   MALNCSPNPLRRLEGKVAIITGGASGIGASAVRIFHENGAKVTIADIQDEAGQKISDELG 60

Query: 61  KDVSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 120
           +DV+Y+HCDVSKE+DV N+VD AV RHGKLDIMY NAGVIDR F GILDVTKSD+DKVLG
Sbjct: 61  EDVNYIHCDVSKEEDVSNVVDAAVYRHGKLDIMYSNAGVIDRSFSGILDVTKSDLDKVLG 120

Query: 121 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 180
           VNVMGAFWGAK AARVMIP+K GCILFTTS   +IAGLS+HPYA+SKCAVLGLVRNL+AE
Sbjct: 121 VNVMGAFWGAKHAARVMIPQKNGCILFTTSATTNIAGLSSHPYASSKCAVLGLVRNLSAE 180

Query: 181 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 240
           LGQHGIRVNCVAPFVVAT I G  +  Q EA+ETMV  WANLKG  LKADDIANAALYLA
Sbjct: 181 LGQHGIRVNCVAPFVVATGIAGPRNPMQAEALETMVTSWANLKGCVLKADDIANAALYLA 240

Query: 241 SDEADYVSGLNLIVDGGYSVVNPSMLNSLKLME 274
           SD+A YVSGLNL+VDGGYSVVNPSML +LK M+
Sbjct: 241 SDDAKYVSGLNLVVDGGYSVVNPSMLKTLKFMD 273

[Close]

BLAST of Lag0032079 vs. TAIR 10
Match: AT2G47140.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 239.2 bits (609), Expect = 3.9e-63
Identity = 132/255 (51.76%), Postives = 175/255 (68.63%), Query Frame = 0


Query: 11  RRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD-VSYVHCD 70
           +RL GK+ IITGGASGIGA +V  F E+GA+VVI D+QDE+GQ +A  IG+D  SY HCD
Sbjct: 4   KRLDGKIVIITGGASGIGAESVRLFTEHGARVVIVDVQDELGQNVAVSIGEDKASYYHCD 63

Query: 71  VSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGVNVMGAFWG 130
           V+ E +V N V   V ++GKLD+++ NAGVI+ PF  ILD+  +++D+ + +N+ G    
Sbjct: 64  VTNETEVENAVKFTVEKYGKLDVLFSNAGVIE-PFVSILDLNLNELDRTIAINLRGTAAF 123

Query: 131 AKQAARVMIPEK-KGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAELGQHGIRV 190
            K AAR M+ +  +G I+ TTSVAA IAG + H Y  SK  +LGL+++ +  LG++GIRV
Sbjct: 124 IKHAARAMVEKGIRGSIVCTTSVAAEIAGTAPHGYTTSKHGLLGLIKSASGGLGKYGIRV 183

Query: 191 NCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLASDEADYVS 250
           N VAPF VATP+       +   +E      ANLKG  LKA  +A AAL+LASDE+ YVS
Sbjct: 184 NGVAPFGVATPLVCNGFKMEPNVVEQNTSASANLKGIVLKARHVAEAALFLASDESAYVS 243

Query: 251 GLNLIVDGGYSVVNP 264
           G NL VDGGYSVV P
Sbjct: 244 GQNLAVDGGYSVVKP 257

[Close]

BLAST of Lag0032079 vs. TAIR 10
Match: AT3G29250.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 233.4 bits (594), Expect = 2.1e-61
Identity = 133/252 (52.78%), Postives = 171/252 (67.86%), Query Frame = 0


Query: 12  RLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD-VSYVHCDV 71
           RL GK+AIITGGASGIGA AV  F ++GAKVVI DIQ+E+GQ +A  IG D  S+  C+V
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLDKASFYRCNV 64

Query: 72  SKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGVNVMGAFWGA 131
           + E DV N V   V +HGKLD+++ NAGV++  FG +LD+     D+ + VNV GA    
Sbjct: 65  TDETDVENAVKFTVEKHGKLDVLFSNAGVLE-AFGSVLDLDLEAFDRTMAVNVRGAAAFI 124

Query: 132 KQAARVMIPE-KKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAELGQHGIRVN 191
           K AAR M+    +G I+ TTS+AA I G   H Y ASK A+LGL+R+  A LGQ+GIRVN
Sbjct: 125 KHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAGLGQYGIRVN 184

Query: 192 CVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLASDEADYVSG 251
            VAP+ VAT +T   +   V+ +E       NLKG  LKA  IA AAL+LASD++ Y+SG
Sbjct: 185 GVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLASDDSVYISG 244

Query: 252 LNLIVDGGYSVV 262
            NL+VDGG+SVV
Sbjct: 245 QNLVVDGGFSVV 255

[Close]

BLAST of Lag0032079 vs. TAIR 10
Match: AT3G29250.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 3.7e-61
Identity = 134/261 (51.34%), Postives = 177/261 (67.82%), Query Frame = 0


Query: 4   NASAASLRRLV-GKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD 63
           N + + LR+++ GK+AIITGGASGIGA AV  F ++GAKVVI DIQ+E+GQ +A  IG D
Sbjct: 34  NKTMSGLRQVLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDIQEELGQNLAVSIGLD 93

Query: 64  -VSYVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGV 123
             S+  C+V+ E DV N V   V +HGKLD+++ NAGV++  FG +LD+     D+ + V
Sbjct: 94  KASFYRCNVTDETDVENAVKFTVEKHGKLDVLFSNAGVLE-AFGSVLDLDLEAFDRTMAV 153

Query: 124 NVMGAFWGAKQAARVMIPE-KKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 183
           NV GA    K AAR M+    +G I+ TTS+AA I G   H Y ASK A+LGL+R+  A 
Sbjct: 154 NVRGAAAFIKHAARSMVASGTRGSIVCTTSIAAEIGGPGPHSYTASKHALLGLIRSACAG 213

Query: 184 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLA 243
           LGQ+GIRVN VAP+ VAT +T   +   V+ +E       NLKG  LKA  IA AAL+LA
Sbjct: 214 LGQYGIRVNGVAPYGVATGMTSAYNEEAVKMLEEYGEALGNLKGVVLKARHIAEAALFLA 273

Query: 244 SDEADYVSGLNLIVDGGYSVV 262
           SD++ Y+SG NL+VDGG+SVV
Sbjct: 274 SDDSVYISGQNLVVDGGFSVV 293

[Close]

BLAST of Lag0032079 vs. TAIR 10
Match: AT1G52340.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 229.9 bits (585), Expect = 2.4e-60
Identity = 130/271 (47.97%), Postives = 173/271 (63.84%), Query Frame = 0


Query: 6   SAASLRRLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKDVS- 65
           S+   +RL+GKVA+ITGGA+GIG S V  FH++GAKV I D+QD++G ++   + +  S 
Sbjct: 11  SSLPSQRLLGKVALITGGATGIGESIVRLFHKHGAKVCIVDLQDDLGGEVCKSLLRGESK 70

Query: 66  ----YVHCDVSKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLG 125
               ++H DV  EDD+ N VD AV   G LDI+  NAG+   P   I + + S+ +    
Sbjct: 71  ETAFFIHGDVRVEDDISNAVDFAVKNFGTLDILINNAGLCGAPCPDIRNYSLSEFEMTFD 130

Query: 126 VNVMGAFWGAKQAARVMIPEKKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAE 185
           VNV GAF   K AARVMIPEKKG I+   SV   + G+  H Y  SK AVLGL R++AAE
Sbjct: 131 VNVKGAFLSMKHAARVMIPEKKGSIVSLCSVGGVVGGVGPHSYVGSKHAVLGLTRSVAAE 190

Query: 186 LGQHGIRVNCVAPFVVATPITGLSDSAQVEAMETMVRGW-------ANLKGQSLKADDIA 245
           LGQHGIRVNCV+P+ VAT +  L+   + E  E    G+       ANLKG  L  DD+A
Sbjct: 191 LGQHGIRVNCVSPYAVATKL-ALAHLPEEERTEDAFVGFRNFAAANANLKGVELTVDDVA 250

Query: 246 NAALYLASDEADYVSGLNLIVDGGYSVVNPS 265
           NA L+LASD++ Y+SG NL++DGG++  N S
Sbjct: 251 NAVLFLASDDSRYISGDNLMIDGGFTCTNHS 280

[Close]

BLAST of Lag0032079 vs. TAIR 10
Match: AT2G47130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 228.4 bits (581), Expect = 6.9e-60
Identity = 131/254 (51.57%), Postives = 168/254 (66.14%), Query Frame = 0


Query: 12  RLVGKVAIITGGASGIGASAVHRFHENGAKVVIADIQDEVGQKIADEIGKD-VSYVHCDV 71
           RL GK+AIITGGASGIGA AV  F ++GAKVVI D Q+E+GQ +A  +GKD  S+  CDV
Sbjct: 5   RLDGKIAIITGGASGIGAEAVRLFTDHGAKVVIVDFQEELGQNVAVSVGKDKASFYRCDV 64

Query: 72  SKEDDVRNLVDTAVSRHGKLDIMYCNAGVIDRPFGGILDVTKSDVDKVLGVNVMGAFWGA 131
           + E +V N V   V ++GKLD+++ NAGV+++P G  LD+     D+ + VNV GA    
Sbjct: 65  TNEKEVENAVKFTVEKYGKLDVLFSNAGVMEQP-GSFLDLNLEQFDRTMAVNVRGAAAFI 124

Query: 132 KQAARVMIPE-KKGCILFTTSVAASIAGLSTHPYAASKCAVLGLVRNLAAELGQHGIRVN 191
           K AAR M+ +  +G I+ TTSVA+ I G   H Y ASK A+LGLV++    LG++GIRVN
Sbjct: 125 KHAARAMVEKGTRGSIVCTTSVASEIGGPGPHAYTASKHALLGLVKSACGGLGKYGIRVN 184

Query: 192 CVAPFVVATPITGLSDSAQVEAMETMVRGWANLKGQSLKADDIANAALYLASDEADYVSG 251
            VAP+ VAT I    D   V  +E        LKG  LKA  +A AAL+LASD++ YVSG
Sbjct: 185 GVAPYAVATAINS-RDEETVRMVEEYSAATGILKGVVLKARHVAEAALFLASDDSAYVSG 244

Query: 252 LNLIVDGGYSVVNP 264
            NL VDGGYSVV P
Sbjct: 245 QNLAVDGGYSVVKP 256

The following BLAST results are available for this feature:

BLAST of Lag0032079 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022934668.1	1.2e-122	83.52	tropinone reductase-like 1 isoform X2 [Cucurbita moschata]	[more]
XP_023527968.1	5.9e-122	83.88	tropinone reductase-like 1 [Cucurbita pepo subsp. pepo]	[more]
KAG6580722.1	5.9e-122	83.46	hypothetical protein SDJN03_20724, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022983067.1	1.3e-121	83.15	tropinone reductase-like 1 [Cucurbita maxima]	[more]
XP_022934667.1	2.3e-121	83.58	tropinone reductase-like 1 isoform X1 [Cucurbita moschata]	[more]

BLAST of Lag0032079 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
H9BFQ0	1.3e-79	57.25	Tropinone reductase-like 1 OS=Erythroxylum coca OX=289672 PE=2 SV=1	[more]
H9BFQ1	2.2e-79	56.46	Tropinone reductase-like 2 OS=Erythroxylum coca OX=289672 PE=2 SV=1	[more]
A3F5F0	1.2e-69	53.90	Secoisolariciresinol dehydrogenase OS=Sinopodophyllum hexandrum OX=93608 GN=SDH ...	[more]
Q7FAE1	7.9e-69	54.62	Momilactone A synthase OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0179200 ...	[more]
Q94KL8	3.9e-68	52.61	Secoisolariciresinol dehydrogenase (Fragment) OS=Podophyllum peltatum OX=35933 P...	[more]

BLAST of Lag0032079 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F8A8	5.8e-123	83.52	tropinone reductase-like 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111441...	[more]
A0A6J1IY89	6.4e-122	83.15	tropinone reductase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111481723 PE=4 SV=1	[more]
A0A6J1F3G3	1.1e-121	83.58	tropinone reductase-like 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441...	[more]
Q9SBM0	2.3e-119	81.68	Wts2L OS=Citrullus lanatus OX=3654 GN=wts2L PE=2 SV=1	[more]
A0A5D3DNU4	1.4e-116	80.22	Tropinone reductase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Lag0032079 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G47140.1	3.9e-63	51.76	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.2	2.1e-61	52.78	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT3G29250.1	3.7e-61	51.34	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G52340.1	2.4e-60	47.97	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT2G47130.1	6.9e-60	51.57	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00080	SDRFAMILY	coord: 88..99 score: 39.68 coord: 163..182 score: 39.8 coord: 143..151 score: 39.79
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 88..99 score: 49.45 coord: 222..242 score: 30.65 coord: 163..182 score: 44.31 coord: 137..153 score: 29.02 coord: 17..34 score: 47.04 coord: 184..201 score: 44.28
None	No IPR available	GENE3D	3.40.50.720		coord: 3..271 e-value: 1.7E-79 score: 268.7
None	No IPR available	PFAM	PF13561	adh_short_C2	coord: 22..259 e-value: 1.5E-64 score: 217.8
None	No IPR available	PIRSR	PIRSR000094-3	PIRSR000094-3	coord: 13..262 e-value: 6.8E-21 score: 72.4
None	No IPR available	PIRSR	PIRSR000095-1	PIRSR000095-1	coord: 19..200 e-value: 1.4E-16 score: 58.3
None	No IPR available	PANTHER	PTHR43180	3-OXOACYL-(ACYL-CARRIER-PROTEIN) REDUCTASE (AFU_ORTHOLOGUE AFUA_6G11210)	coord: 9..269
None	No IPR available	PANTHER	PTHR43180:SF37	SECOISOLARICIRESINOL DEHYDROGENASE-LIKE	coord: 9..269
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 15..261

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0032079.1	Lag0032079.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity