Lag0030702 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0030702 vs. NCBI nr
Match: RVW64408.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 679.1 bits (1751), Expect = 8.0e-191
Identity = 360/905 (39.78%), Postives = 503/905 (55.58%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            ETK  ++D+  + S+W  K V W  + + G  GG++ILWD SKL+  E + G        
Sbjct: 771  ETKRETWDRRFVSSVWKGKRVEWAALPACGASGGIVILWDSSKLECTEKVLGSFSVTVKF 830

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNV R I E++  +R
Sbjct: 831  NSGEEGSFWLTSVYGPINPLWRKDFWLELQDLYGLTFPRWCVGGDFNVIRRISEKLGETR 890

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             T  MR F++ I E GL++ PL N  FTWS    D     +DRFL S EWD  F  S   
Sbjct: 891  LTLNMRCFDEFIRESGLIDPPLRNAAFTWSNMQADPICKRLDRFLFSSEWDTFFSQSFQE 950

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
               R  SDH P+ LE     WGP+PFR  N WL   +  +   +   +    GW G    
Sbjct: 951  ALPRWTSDHSPICLETNPLKWGPTPFRFENMWLLHPEFKEKFRVWWLECTGEGWEGHKFM 1010

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ +K  +K W  +   + K+++K +L +++  D   ++  L+S+ +  RT  R EL 
Sbjct: 1011 RKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRRELE 1070

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
            D+ L EE    QK ++ W+K GD N+ FFHR    ++ +  I  L S  G +L    +I 
Sbjct: 1071 DVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDIS 1130

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E++ FF  LY     +       DW  +S      L  PF  EE+   +  L K KAPG
Sbjct: 1131 EEIVNFFGNLYSKPVGESWRVEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAPG 1190

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GFT   + + W+ +K D +R+F E H NG +N      FI L+ K+   + I D+RPI
Sbjct: 1191 PDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRPI 1250

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +S+YKI+AKVL+ RL+KV+   IS  Q AFVEGR ILD +LIANE V+  R   ++G
Sbjct: 1251 SLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEEG 1310

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
             + K+D EKA+D VDW FLD VL  KGF +KW  WI+GC+ +  F++ +NG  +G + AS
Sbjct: 1311 IVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKAS 1370

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF L+++V S ++ +    G  EGF VG+D+  VS+LQFADDTI F K
Sbjct: 1371 RGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFSK 1430

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
                    L   + +F   SGLKIN EKS + GIN     +   AS  +C+V   P +YL
Sbjct: 1431 ASMEHLQNLKIILLVFGQVSGLKINLEKSTISGINTRQELLSSLASVFDCRVSEWPLSYL 1490

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            GLPLGG+PK   FW PV++++ +++D WK+  LS GGR+TL  S LS IP YFLSLF + 
Sbjct: 1491 GLPLGGNPKTIGFWDPVVERISRRLDGWKKAYLSLGGRITLIQSCLSHIPSYFLSLFKIP 1550

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
            +SI+  ++++ R+F W G    K +HLV W +VS  ++ GGLG G ++ RN+ALL KW W
Sbjct: 1551 ASIASKIEKMQRNFLWSGAGEGKKDHLVRWEVVSRPKELGGLGFGKISLRNIALLGKWLW 1610

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            RF  E    W +VI +IYGT   GW++      S R PW +IA+++Q F       +GNG
Sbjct: 1611 RFPRERSGLWYKVIGSIYGTHPNGWDANMVVRWSHRCPWKAIAQVFQEFSPFVRLVVGNG 1670

BLAST of Lag0030702 vs. NCBI nr
Match: RVW65579.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 664.5 bits (1713), Expect = 2.0e-186
Identity = 344/905 (38.01%), Postives = 509/905 (56.24%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            ETK    D+ L+ S+WS ++  W  + + G  GG+LI+WD  K++  E + G        
Sbjct: 36   ETKKEECDRRLVGSVWSVRNKDWAALPASGASGGILIIWDSIKMRREEVVLGSFSVSIKF 95

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNV R   E++  SR
Sbjct: 96   AMDGCESLWLSAVYGPNNSALRKDFWVELSDIAGLSHPRWCVGGDFNVIRRSSEKLGGSR 155

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             T  M+ F++ I +  L++ PL +  +TWS   ++     +DRFL S EW+ +F  S   
Sbjct: 156  LTPCMKDFDEFIRDCELIDSPLRSVSYTWSNMQENPVCKRLDRFLYSNEWEQVFPQSLQG 215

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
               R  SDH+P++LE   F WGP+PFR  N WL  +   +      S+ +  GW G    
Sbjct: 216  VLPRWTSDHWPIVLETNPFKWGPTPFRFENMWLQHSSFKENFGRWWSEFQGNGWEGHKFM 275

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ +K  +K W      E  +K+K +L  +A FD+  ++  LS E +  R F + EL 
Sbjct: 276  RKLQFVKAKLKEWNKTSFGELSKKKKDILAVLANFDSLEQEGGLSQELLVQRAFSKGELE 335

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
            +L L EE +  QK ++ W+K GD N+ FFH+    ++ +  I  L +  G  L     I+
Sbjct: 336  ELILREEIHWRQKARVKWVKKGDCNSKFFHKVANGRRNRKFIKELENESGLMLNNPESIK 395

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E+L +F KLY     +       DWS +     S L +PF  EEI+  +  + ++KAPG
Sbjct: 396  EEILKYFEKLYACPSRESWRVEGLDWSPIDGESASRLESPFTEEEIYKAIFQMDRDKAPG 455

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GFT   F   W+ +K D +R+F+E HR+G +N     +FI L+ K+ +   I DFRPI
Sbjct: 456  PDGFTIAVFQDCWDVIKEDLVRVFAEFHRSGIINQSTNASFIVLLPKKSISRRISDFRPI 515

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +S+YKI+AKVLA RL+ V+   I   Q AFV+GRQILD +LIANE V+  R   ++G
Sbjct: 516  SLITSLYKIIAKVLAGRLRGVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKRRTGEEG 575

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
             + K+D EKA+D V WDFLD VL  KGF  +W +W++GC+ +  ++V +NG  +G + AS
Sbjct: 576  VVFKIDFEKAYDHVSWDFLDHVLEMKGFSLRWRKWMRGCLSSVSYAVLVNGNAKGWVKAS 635

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF ++++V S ++ K   +   EGF VG+++  VS LQFADDTI F  
Sbjct: 636  RGLRQGDPLSPFLFTIVADVLSRMLLKAEERNVLEGFRVGRNRTRVSHLQFADDTIFFSS 695

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
              +    TL   + +F   SGLK+N +KS + GIN++   +   A  ++CK    P  YL
Sbjct: 696  TREEDLMTLKSVLLVFGHISGLKVNLDKSNIYGINIEQNHLSRLAVMLDCKASGWPILYL 755

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            GLPLGG+PK   FW PV++++ +++D W++  LS GGR+TL  S L+ +P YFLSLF + 
Sbjct: 756  GLPLGGNPKASGFWDPVIERISRRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFRIP 815

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
            +S++  ++R+ R F W G    K +HLV W +V   +  GGLG G ++ RN+ALL KW W
Sbjct: 816  ASVAAKIERMQREFLWSGVGEGKRDHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLW 875

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            R+  E  + W +VI++IYG+   GW+  N    S R PW +IA ++Q F     F +G+G
Sbjct: 876  RYPREGSALWHQVILSIYGSHSNGWDVNNNVRWSHRCPWKAIALVFQEFSKFTRFVVGDG 935

BLAST of Lag0030702 vs. NCBI nr
Match: RVW99790.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 662.1 bits (1707), Expect = 1.0e-185
Identity = 344/905 (38.01%), Postives = 510/905 (56.35%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            ETK    D+ L+ S+WS ++  W  + + G  GG+LI+WD  KL+  E + G        
Sbjct: 393  ETKKEECDRRLVGSVWSVRNKDWAALPASGASGGILIIWDSKKLRREEVVLGSFSVSIKF 452

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNV R   E++  SR
Sbjct: 453  AMDGCESLWLSSVYGPNNSALRKDFWVELSDIAGLSHPRWCVGGDFNVIRRSSEKLGGSR 512

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             +  M+ F++ I +  L++ PL +  +TWS   ++     +DRFL S EW+ +F  S   
Sbjct: 513  LSPCMKDFDEFIRDCELIDSPLRSASYTWSNMQENPVCKRLDRFLYSNEWEQVFPQSLQG 572

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
               R  SDH+P++LE   F WGP+PF+  N WL  +   +      S+ +  GW G    
Sbjct: 573  VLPRWTSDHWPIVLETNPFKWGPTPFKFENMWLQHSSFKENFGRWWSEFQGNGWEGHKFM 632

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ +K  +K W      E  +K+K +L  +A FD+  ++  LS E +  R F + EL 
Sbjct: 633  RKLQFVKAKLKEWNKTSFGELSKKKKDILAVLANFDSLEQEGGLSHELLVQRAFSKGELE 692

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
            +L L EE +  QK ++ W+K GD N+NFFH+    ++ +  I  L +  G  L     I+
Sbjct: 693  ELILREEIHWRQKARVKWVKEGDCNSNFFHKVANGRRNRKFIKELENESGLMLNNPESIK 752

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E+L +F KLY +   +       DWS +     S L +PF  EEI+  +  + ++KAPG
Sbjct: 753  EEILKYFEKLYVSPSGESWRVEGLDWSPIDGESASRLESPFTEEEIYKAIFQMDRDKAPG 812

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+ FT   F   W+ +K D +R+F+E HR+G +N     +FI LI K+ +   I DFRPI
Sbjct: 813  PDDFTIAVFQDCWDVIKEDLVRVFTEFHRSGIINQSTNASFIVLIPKKSMSRRISDFRPI 872

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +S+Y+I+AKVLA RL+ V+   I   Q AFV+GRQILD +LIANE V+  R   ++G
Sbjct: 873  SLITSLYEIIAKVLAGRLRGVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKRRTGEEG 932

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
             + K+D EKA+D V WDFLD VL  KGF  +W +W++GC+ +  ++V +NG  +G + AS
Sbjct: 933  VVFKIDFEKAYDHVSWDFLDHVLEMKGFSLRWRKWMRGCLSSVSYAVLVNGNAKGWVKAS 992

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF ++++V S ++ K   +   EGF VG+++  VS LQFADDTI F  
Sbjct: 993  RGLRQGDPLSPFLFTIVADVLSRMLLKAEERNVLEGFRVGRNRTRVSHLQFADDTIFFSS 1052

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
              +    TL   + +F   SGLK+N +KS + GINL+   +   A  ++CK    P  YL
Sbjct: 1053 TREEDLMTLKSVLLVFGHISGLKVNLDKSNIYGINLEQNHLSRLAVMLDCKASGWPILYL 1112

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            GLPLGG+PK   FW PV++++ +++D W++  LS GGR+TL  S L+ +P YFLSLF + 
Sbjct: 1113 GLPLGGNPKASGFWDPVIERISRRLDVWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIP 1172

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
            +S++  ++R+ R F W G    K +HLV W +V   +  GGLG G ++ RN+ALL KW W
Sbjct: 1173 ASVAAKIERMQREFLWSGVGEGKRDHLVNWDVVCKPKSRGGLGFGKISMRNVALLGKWLW 1232

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            R+  E  + W +VI++IYG+   GW+  N    S R PW +IA ++Q F     F +G+G
Sbjct: 1233 RYRREGSALWHQVILSIYGSHSNGWDVNNNVRWSHRCPWKAIALVFQEFSKFTRFVVGDG 1292

BLAST of Lag0030702 vs. NCBI nr
Match: RVX13544.1 (LINE-1 retrotransposable element ORF2 protein [Vitis vinifera])

HSP 1 Score: 659.1 bits (1699), Expect = 8.5e-185
Identity = 340/905 (37.57%), Postives = 507/905 (56.02%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            ETK    D+  + S+W++++  W  + + G  GG+LI+WD  KL   E + G        
Sbjct: 362  ETKKEECDRRFVGSVWTARNKDWATLPACGASGGILIIWDTKKLSREEVMLGSFSVSIKF 421

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNV R   E++  SR
Sbjct: 422  TLNGCESLWLSAVYGPNNSALRKDLWVELSDIAGLASPRWCVGGDFNVIRRSSEKLGGSR 481

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             T  M+ F+  I++  L++LPL +  FTWS    +     +DRFL S EW+  F  S   
Sbjct: 482  LTPSMKDFDDFISDCELIDLPLRSASFTWSNMQVNPVCKRLDRFLYSNEWEQTFPQSIQG 541

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
               R  SDH+P++LE   F WGP+PFR  N WL      +       + +  GW G    
Sbjct: 542  VLPRWTSDHWPIVLETNPFKWGPTPFRFENMWLQHPSFKENFGRWWREFQGNGWEGHKFM 601

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ +K  +K W      E  ++++ +L  +  FD+  ++  LS E ++ R   + EL 
Sbjct: 602  RKLQFVKAKLKVWNKASFGELSKRKEDILSALVNFDSLEQEGGLSHELLAQRAIKKGELE 661

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
            +L L EE +  QK ++ W+K GD N+ FFH+    ++ +  I  L + +G  +  +  I+
Sbjct: 662  ELILREEIHWRQKARVKWVKEGDCNSKFFHKVANGRRNRKFIKELENENGQMMNNSESIK 721

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E+L +F KLY +   +       DWS +S      L +PF  EEI   +  + ++KAPG
Sbjct: 722  EEILRYFEKLYTSPSGESWRVEGLDWSPISGESAVRLESPFTEEEICKAIFQMDRDKAPG 781

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GFT   F   WE +K D +++F+E HR+G +N     +FI L+ K+ +   I DFRPI
Sbjct: 782  PDGFTIAVFQDCWEVIKEDLVKVFTEFHRSGIINQSTNASFIVLLPKKSMSRRISDFRPI 841

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +S+YKI+AKVLA R+++V+   I   Q AFV+GRQILD +LIANE V+  R   ++G
Sbjct: 842  SLITSLYKIIAKVLAGRIREVLHETIHSTQGAFVQGRQILDAVLIANEIVDEKRRSGEEG 901

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
             + K+D EKA+D V WDFLD V+  KGF  +W +W++GC+ +  F+V +NG  +G + AS
Sbjct: 902  VVFKIDFEKAYDHVSWDFLDHVMEMKGFGIRWRKWMRGCLSSVSFAVLVNGNAKGWVKAS 961

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF ++++V S ++ K   +   EGF VG+++  VS LQFADDTI F  
Sbjct: 962  RGLRQGDPLSPFLFTIVADVLSRMLLKAEERNVLEGFKVGRNRTRVSHLQFADDTIFFSS 1021

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
              +    TL   + +F   SGLK+N +KS + GINL+   +   A  ++CK    P  YL
Sbjct: 1022 SREEDMMTLKNVLLVFGHISGLKVNLDKSNIYGINLEQNHLSRLAEMLDCKASGWPILYL 1081

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            GLPLGG+PK   FW PV++++ +++D W++  LS GGR+TL  S L+ +P YFLSLF + 
Sbjct: 1082 GLPLGGNPKTSGFWDPVIERISRRLDGWQKAYLSFGGRITLIQSCLTHMPCYFLSLFKIP 1141

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
            +S++  ++R+ R F W G    K +HLV W +V   +  GGLG G ++ RN+ALL KW W
Sbjct: 1142 ASVAAKIERMQRDFLWSGVGEGKRDHLVNWDVVCKPKSRGGLGFGKISIRNVALLGKWLW 1201

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            R+  E  + W +VI++IYG+   GW+  N    S R PW +IA ++Q F     F +GNG
Sbjct: 1202 RYPREGSALWHQVILSIYGSHSNGWDVNNTVRWSHRCPWKAIALVYQEFSKFTRFVVGNG 1261

BLAST of Lag0030702 vs. NCBI nr
Match: RVX23556.1 (Transposon TX1 uncharacterized 149 kDa protein [Vitis vinifera])

HSP 1 Score: 659.1 bits (1699), Expect = 8.5e-185
Identity = 345/878 (39.29%), Postives = 506/878 (57.63%), Query Frame = 0

Query: 499  IQLKEIQPFLPHETKMVSFDQCLIKSIWSSKDVGWVNVKSWG-----------RLGGLLI 558
            ++L++    +  ETK    D+ L+ S+W+ ++  W  + + G           R    + 
Sbjct: 47   LRLEKPDVVMIQETKKEKCDRRLVGSVWTVRNKDWDFLPACGASVYGPNSPSLRKDFWVE 106

Query: 559  LWDESKLKIVEFLQGGGDFNVTRWIHERIPVSRATRGMRQFNKLINELGLLELPLSNGKF 618
            L+D   L    +   GGDFNV R   E++  SR T  MR F+  I+E  LL+ PL N  F
Sbjct: 107  LYDICGLTFPLWCV-GGDFNVIRRSSEKLGGSRLTSSMRDFDSFISESELLDPPLRNASF 166

Query: 619  TWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVSKQVRTISDHFPLLLEAGNFVWGPSPFR 678
            TWS   +      +DRFL S EW  +F        +R  SDH+P+ L+   F WGP+PFR
Sbjct: 167  TWSNMQESPVCKRLDRFLYSNEWGQLFPQGTQETLIRRTSDHWPIALDTNPFTWGPTPFR 226

Query: 679  VFNSWLNMADCIKIVELTLSQDKSYGWVGFVIASKLRKLKINIKNWFAVFERERKQKEKS 738
              N WL      +         +  GW G     +L+ +K   K W  +      +K+KS
Sbjct: 227  FENMWLQHPSFKENFRNWWRGFQGNGWEGHKFMRRLQFVKAKAKEWNKLSFGVLNEKKKS 286

Query: 739  LLDEIAWFDAKAEDNQLSSEEISLRTFVRSELLDLYLVEERNSIQKCKLLWLKAGDENTN 798
            +L ++A  DA  +D  L+SE +  R   + EL DL L EE +  QK ++ W+K GD N+ 
Sbjct: 287  ILKDLANLDAIEQDGGLTSELLGQRALRKGELEDLILREEIHWRQKARVKWVKEGDCNSK 346

Query: 799  FFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIEFEVLGFFTKLYQALPEKRVFPFNFDWS 858
            FFH+    ++ +  I  L +  G  L  A  I  E+L +F KLY     +       DWS
Sbjct: 347  FFHKVANGRRNRKYIKSLENETGLVLNNAMSITEEILLYFEKLYANPIGESWSIEGLDWS 406

Query: 859  MVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPGPEGFTSEFFIKFWEFLKADFIRLFSEL 918
             +S+    +L+APF  EEI   +  + ++KAPGP+GFT   F   W+ +K D +R+F+E 
Sbjct: 407  PISEESAISLVAPFTEEEISKAIFKMDRDKAPGPDGFTIAVFQDCWDVIKEDLVRVFAEF 466

Query: 919  HRNGHLNSCLKENFICLIQKEEVVLTIKDFRPISLTSSVYKILAKVLAKRLKKVIPSIIS 978
            HR+G +N     +FI L+ K+     I DFRPISL +S+YKI+AKVL+ RL+ V+   I 
Sbjct: 467  HRSGVINQSTNASFIVLLPKKSTTKKISDFRPISLITSLYKIIAKVLSGRLRGVLHETIH 526

Query: 979  PYQSAFVEGRQILDPILIANEAVEYYRVKNKKGWILKLDIEKAFDCVDWDFLDKVLCFKG 1038
              Q AFV+GRQI+D +LIANE V+  R   ++G + K+D EKA+D V WDFLD+VL  KG
Sbjct: 527  STQGAFVQGRQIMDAVLIANEIVDERRRSGEEGVVFKIDFEKAYDHVRWDFLDQVLEKKG 586

Query: 1039 FEKKWIQWIQGCVRNPKFSVFINGRPRGRIVASRGLRQGDPLSPFLFLLISEVFSALVDK 1098
            F  KW +W+ GC+ +  ++V +NG  +G + ASRGLRQGDPLSPFLF L+++V S ++ +
Sbjct: 587  FSPKWRKWMNGCLSSVSYAVLVNGSAKGWVKASRGLRQGDPLSPFLFTLVADVLSRMLVR 646

Query: 1099 IHLKGAFEGFLVGQDKVHVSILQFADDTILFCKDDDGMFNTLIQTIELFEWCSGLKINWE 1158
               +   EGF VG+++  VS LQFADDTI F    +    TL   +  F   SGLK+N +
Sbjct: 647  AEERNMLEGFRVGRNRTRVSHLQFADDTIFFSNTREEDLQTLKSLLLAFGHISGLKVNLD 706

Query: 1159 KSALCGINLDDAKVCHFASRINCKVEVLPFNYLGLPLGGHPKKYSFWQPVLDKVQKKIDR 1218
            KS + GINLD A +   A  + CK    P  YLGLPLGG+P+   FW PV++++ +++D 
Sbjct: 707  KSNIYGINLDHAHMSRLAETLGCKASGWPILYLGLPLGGNPRAGGFWDPVIERISRRLDG 766

Query: 1219 WKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLSSSISINLDRILRSFFWEGNEGSKVNHL 1278
            W++  LS GGR+TL  S L+ +P Y+LSLF L +S++  ++R+ R F W G    K +HL
Sbjct: 767  WQKAYLSFGGRITLIHSCLTHMPCYYLSLFKLPASVAAKIERLQRDFLWSGIGEGKKDHL 826

Query: 1279 VGWSLVSNSQKNGGLGIGALNQRNMALLAKWGWRFMMEPHSFWRRVIVNIYGTSKFGWNS 1338
            V W +V N ++ GGLG G ++ RN+ALL KW WR+  E  + W +VI++IYG+   GW++
Sbjct: 827  VRWDVVCNPKERGGLGFGNISLRNLALLGKWLWRYPREGSALWHQVILSIYGSHSNGWDA 886

Query: 1339 ENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNGMKIRF 1366
                  S R PW +I++++Q F S   F +GNG +IRF
Sbjct: 887  NTIVRWSHRCPWKAISQVFQEFSSFTRFVVGNGERIRF 923

BLAST of Lag0030702 vs. ExPASy Swiss-Prot
Match: O00370 (LINE-1 retrotransposable element ORF2 protein OS=Homo sapiens OX=9606 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 3.0e-47
Identity = 183/768 (23.83%), Postives = 334/768 (43.49%), Query Frame = 0

Query: 564  GDFNVTRWIHERIPVSRATRGMRQFNKLINELGLLEL-----PLSNGKFTWSRPGDDSSQ 623
            GDFN    I +R    +  +  ++ N  +++  L+++     P S     +S P    + 
Sbjct: 144  GDFNTPLSILDRSTRQKVNKDTQELNSALHQTDLIDIYRTLHPKSTEYTFFSAP--HHTY 203

Query: 624  SLIDRFLISKEWDVMFDNSRVSKQVRTISDHFPLLLE--------AGNFVWGPSPFRVFN 683
            S ID  + SK   ++    R       +SDH  + LE        + +  W  +   + +
Sbjct: 204  SKIDHIVGSKA--LLSKCKRTEIITNYLSDHSAIKLELRIKNLTQSRSTTWKLNNLLLND 263

Query: 684  SWLN---MADCIKIVELTLSQDKSYG--WVGFVIASKLRKLKINIKNWFAVFERERKQKE 743
             W++    A+     E   ++D +Y   W  F         K   +  F      ++++E
Sbjct: 264  YWVHNEMKAEIKMFFETNENKDTTYQNLWDAF---------KAVCRGKFIALNAYKRKQE 323

Query: 744  KSLLDEIAWFDAKAEDNQLSSEEISLR---TFVRSELLDLYLVEERNSIQKCKLLWLKAG 803
            +S +D +     + E  + +  + S R   T +R+EL ++   +    I + +  + +  
Sbjct: 324  RSKIDTLTSQLKELEKQEQTHSKASRRQEITKIRAELKEIETQKTLQKINESRSWFFERI 383

Query: 804  DENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIEFEVLGFFTKLY----QALPEKR 863
            ++      R +  K+ K  I  + +  G       EI+  +  ++  LY    + L E  
Sbjct: 384  NKIDRPLARLIKKKREKNQIDTIKNDKGDITTDPTEIQTTIREYYKHLYANKLENLEEMD 443

Query: 864  VFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPGPEGFTSEFFIKFWEFLKA 923
             F   +    ++Q +  +L  P    EI   + +L   K+PGP+GFT+EF+ ++ E L  
Sbjct: 444  TFLDTYTLPRLNQEEVESLNRPITGSEIVAIINSLPTKKSPGPDGFTAEFYQRYKEELVP 503

Query: 924  DFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKD-FRPISLTSSVYKILAKVLAKR 983
              ++LF  + + G L +   E  I LI K     T K+ FRPISL +   KIL K+LA R
Sbjct: 504  FLLKLFQSIEKEGILPNSFYEASIILIPKPGRDTTKKENFRPISLMNIDAKILNKILANR 563

Query: 984  LKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYY-RVKNKKGWILKLDIEKAFDCVDW 1043
            +++ I  +I   Q  F+ G Q    I  +   +++  R K+K   I+ +D EKAFD +  
Sbjct: 564  IQQHIKKLIHHDQVGFIPGMQGWFNIRKSINVIQHINRAKDKNHVIISIDAEKAFDKIQQ 623

Query: 1044 DFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVASRGLRQGDPLSPFLFLL 1103
             F+ K L   G +  +++ I+     P  ++ +NG+         G RQG PLSP LF +
Sbjct: 624  PFMLKTLNKLGIDGMYLKIIRAIYDKPTANIILNGQKLEAFPLKTGTRQGCPLSPLLFNI 683

Query: 1104 ISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCKDDDGMFNTLIQTIELF 1163
            + EV   L   I  +   +G  +G+++V +S+  FADD I++ ++       L++ I  F
Sbjct: 684  VLEV---LARAIRQEKEIKGIQLGKEEVKLSL--FADDMIVYLENPIVSAQNLLKLISNF 743

Query: 1164 EWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYLGLPLGGHPKKY--SFW 1223
               SG KIN +KS     N +          +   +      YLG+ L    K      +
Sbjct: 744  SKVSGYKINVQKSQAFLYNNNRQTESQIMGELPFTIASKRIKYLGIQLTRDVKDLFKENY 803

Query: 1224 QPVLDKVQKKIDRWKRINLSRGGRLTLCS-SVLSSIPLYFLSL-FLLSSSISINLDRILR 1283
            +P+L ++++  ++WK I  S  GR+ +   ++L  +   F ++   L  +    L++   
Sbjct: 804  KPLLKEIKEDTNKWKNIPCSWVGRINIVKMAILPKVIYRFNAIPIKLPMTFFTELEKTTL 863

Query: 1284 SFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1301
             F W           +  S++S   K GG+ +        A + K  W
Sbjct: 864  KFIWNQKRAR-----IAKSILSQKNKAGGITLPDFKLYYKATVTKTAW 888

BLAST of Lag0030702 vs. ExPASy Swiss-Prot
Match: P08548 (LINE-1 reverse transcriptase homolog OS=Nycticebus coucang OX=9470 PE=4 SV=1)

HSP 1 Score: 187.6 bits (475), Expect = 9.5e-46
Identity = 187/777 (24.07%), Postives = 336/777 (43.24%), Query Frame = 0

Query: 564  GDFNVTRWIHERIPVSRATRGMRQFNKLINELGLLEL----PLSNGKFTWSRPGDDSSQS 623
            GDFN    + +R    + ++ +   N  I  L L ++      +  ++T+       + S
Sbjct: 143  GDFNTPLAVLDRSSKKKLSKEILDLNSTIQHLDLTDIYRTFHPNKTEYTFFSSA-HGTYS 202

Query: 624  LIDRFLISKEWDVMFDNSRVSKQVRTISDHFPLLLEAGN--------FVWGPSPFRVFNS 683
             ID  L  K     F    +   +   SDH  + +E  N          W  +   + ++
Sbjct: 203  KIDHILGHKSNLSKFKKIEIIPCI--FSDHHGIKVELNNNRNLHTHTKTWKLNNLMLKDT 262

Query: 684  WL---NMADCIKIVELTLSQDKSYGWVGFVIASKLRKLKINIKNWFAVFERERKQKEKSL 743
            W+      +  K +E   +QD +Y  +     + LR   I ++   A  ++  +++  +L
Sbjct: 263  WVIDEIKKEITKFLEQNNNQDTNYQNLWDTAKAVLRGKFIALQ---AFLKKTEREEVNNL 322

Query: 744  LDEIAWFDAKAEDNQLSSEEISLRTFVRSELLDLYLVEERNSIQKCKLLWLKAGDENTNF 803
            +  +   + +   N   S    + T +R+EL ++        I K K  + +  ++    
Sbjct: 323  MGHLKQLEKEEHSNPKPSRRKEI-TKIRAELNEIENKRIIQQINKSKSWFFEKINKIDKP 382

Query: 804  FHRFLAAKKRKLLITGLNSIDGSSLLTAGEIEFEVLGFFTKL----YQALPEKRVFPFNF 863
                   K+ K LI+ + + +        EI+  +  ++ KL    Y+ L E   +    
Sbjct: 383  LANLTRKKRVKSLISSIRNGNDEITTDPSEIQKILNEYYKKLYSHKYENLKEIDQYLEAC 442

Query: 864  DWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPGPEGFTSEFFIKFWEFLKADFIRLF 923
                +SQ +   L  P    EI   ++NL K K+PGP+GFTSEF+  F E L    + LF
Sbjct: 443  HLPRLSQKEVEMLNRPISSSEIASTIQNLPKKKSPGPDGFTSEFYQTFKEELVPILLNLF 502

Query: 924  SELHRNGHLNSCLKENFICLIQKEEVVLTIKD-FRPISLTSSVYKILAKVLAKRLKKVIP 983
              + + G L +   E  I LI K     T K+ +RPISL +   KIL K+L  R+++ I 
Sbjct: 503  QNIEKEGILPNTFYEANITLIPKPGKDPTRKENYRPISLMNIDAKILNKILTNRIQQHIK 562

Query: 984  SIISPYQSAFVEGRQILDPILIANEAVEYY-RVKNKKGWILKLDIEKAFDCVDWDFLDKV 1043
             II   Q  F+ G Q    I  +   +++  ++KNK   IL +D EKAFD +   F+ + 
Sbjct: 563  KIIHHDQVGFIPGSQGWFNIRKSINVIQHINKLKNKDHMILSIDAEKAFDNIQHPFMIRT 622

Query: 1044 LCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVASRGLRQGDPLSPFLFLLISEVFS 1103
            L   G E  +++ I+     P  ++ +NG          G RQG PLSP LF ++ EV  
Sbjct: 623  LKKIGIEGTFLKLIEAIYSKPTANIILNGVKLKSFPLRSGTRQGCPLSPLLFNIVMEV-- 682

Query: 1104 ALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCKDDDGMFNTLIQTIELFEWCSGL 1163
             L   I  + A +G  +G +++ +S+  FADD I++ ++       L++ I+ +   SG 
Sbjct: 683  -LAIAIREEKAIKGIHIGSEEIKLSL--FADDMIVYLENTRDSTTKLLEVIKEYSNVSGY 742

Query: 1164 KINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYLGLPLGGHPKKY--SFWQPVLDK 1223
            KIN  KS       ++         I   V      YLG+ L    K      ++ +  +
Sbjct: 743  KINTHKSVAFIYTNNNQAEKTVKDSIPFTVVPKKMKYLGVYLTKDVKDLYKENYETLRKE 802

Query: 1224 VQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLSSSISI--NLDRILRSFFWEG 1283
            + + +++WK I  S  GR+ +    +    +Y  +   + + +S   +L++I+  F W  
Sbjct: 803  IAEDVNKWKNIPCSWLGRINIVKMSILPKAIYNFNAIPIKAPLSYFKDLEKIILHFIWNQ 862

Query: 1284 NEGSKVNHLVGWSLVSNSQKNGGLGIGALN--QRNMALLAKWGWRFMMEPHSFWRRV 1314
             +       +  +L+SN  K GG+ +  L    +++ +   W W    E    W R+
Sbjct: 863  KKPQ-----IAKTLLSNKNKAGGITLPDLRLYYKSIVIKTAWYWHKNREV-DVWNRI 901

BLAST of Lag0030702 vs. ExPASy Swiss-Prot
Match: P14381 (Transposon TX1 uncharacterized 149 kDa protein OS=Xenopus laevis OX=8355 PE=4 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 3.1e-44
Identity = 194/752 (25.80%), Postives = 320/752 (42.55%), Query Frame = 0

Query: 563  GGDFNVTRWIHERIPVSRATRGMRQFNKLINELGLLELPLSNG----KFTWSRPGDDS-S 622
            GGDFN T    +R    +         +LI    L+++          FT+ R  D   S
Sbjct: 141  GGDFNYTLDARDRNVPKKRDSSESVLRELIAHFSLVDVWREQNPETVAFTYVRVRDGHVS 200

Query: 623  QSLIDRFLISKEWDVMFDNSRVSKQVRTISDH--FPLLLEAGNFVWGPSPFRVFNSWLNM 682
            QS IDR  IS        +S +  ++   SDH    L +     +   + +   NS L  
Sbjct: 201  QSRIDRIYISSHLMSRAQSSTI--RLAPFSDHNCVSLRMSIAPSLPKAAYWHFNNSLLED 260

Query: 683  ADCIKIVELTLSQDKSYGWVGFV----------IASKLRKLKINIKNWFAVFERERKQKE 742
                K V     +D   GW  F              K+  LK+  + +      +R  + 
Sbjct: 261  EGFAKSV-----RDTWRGWRAFQDEFATLNQWWDVGKVH-LKLLCQEYTKSVSGQRNAEI 320

Query: 743  KSLLDEIAWFDAK---AEDNQLSSEEISLRTFVRSELLDLYLVEERNSIQKCKLLWLKAG 802
            ++L  E+   + +   +ED  L  E +  +  +R    ++   + R +  + ++  L   
Sbjct: 321  EALNGEVLDLEQRLSGSEDQALQCEYLERKEALR----NMEQRQARGAFVRSRMQLLCDM 380

Query: 803  DENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIEFEVLGFFTKLYQALPEKRVFPF 862
            D  + FF+     K  +  IT L + DG+ L     I      F+  L+   P   + P 
Sbjct: 381  DRGSRFFYALEKKKGNRKQITCLFAEDGTPLEDPEAIRDRARSFYQNLFSPDP---ISPD 440

Query: 863  NFD--WS---MVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPGPEGFTSEFFIKFWEFLK 922
              +  W    +VS+ +   L  P  ++E+   L+ +  NK+PG +G T EFF  FW+ L 
Sbjct: 441  ACEELWDGLPVVSERRKERLETPITLDELSQALRLMPHNKSPGLDGLTIEFFQFFWDTLG 500

Query: 923  ADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPISLTSSVYKILAKVLAKR 982
             DF R+ +E  + G L    +   + L+ K+  +  IK++RP+SL S+ YKI+AK ++ R
Sbjct: 501  PDFHRVLTEAFKKGELPLSCRRAVLSLLPKKGDLRLIKNWRPVSLLSTDYKIVAKAISLR 560

Query: 983  LKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKGWILKLDIEKAFDCVDWD 1042
            LK V+  +I P QS  V GR I D + +  + + + R        L LD EKAFD VD  
Sbjct: 561  LKSVLAEVIHPDQSYTVPGRTIFDNVFLIRDLLHFARRTGLSLAFLSLDQEKAFDRVDHQ 620

Query: 1043 FLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVASRGLRQGDPLSPFLFLLI 1102
            +L   L    F  +++ +++    + +  V IN      +   RG+RQG PLS  L+ L 
Sbjct: 621  YLIGTLQAYSFGPQFVGYLKTMYASAECLVKINWSLTAPLAFGRGVRQGCPLSGQLYSLA 680

Query: 1103 SEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCKDDDGMFNTLIQTIELFE 1162
             E F  L     L+    G ++ +  + V +  +ADD IL  +D   +     +  E++ 
Sbjct: 681  IEPFLCL-----LRKRLTGLVLKEPDMRVVLSAYADDVILVAQDLVDL-ERAQECQEVYA 740

Query: 1163 WCSGLKINWEKSALCGINLDDAKVCHFASRI-NCKVEVLPFNYLGLPLGG--HPKKYSFW 1222
              S  +INW KS+  G+     KV        +   E     YLG+ L    +P   +F 
Sbjct: 741  AASSARINWSKSS--GLLEGSLKVDFLPPAFRDISWESKIIKYLGVYLSAEEYPVSQNFI 800

Query: 1223 QPVLDKVQKKIDRWKRIN--LSRGGRLTLCSSVLSSIPLYFLSLFLLSSSISINLDRILR 1282
            + + + V  ++ +WK     LS  GR  + + +++S   Y L     +      + R L 
Sbjct: 801  E-LEECVLTRLGKWKGFAKVLSMRGRALVINQLVASQIWYRLICLSPTQEFIAKIQRRLL 860

Query: 1283 SFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGI 1285
             F W G       H V   + S   K GG G+
Sbjct: 861  DFLWIG------KHWVSAGVSSLPLKEGGQGV 862

BLAST of Lag0030702 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 181.4 bits (459), Expect = 6.8e-44
Identity = 185/780 (23.72%), Postives = 335/780 (42.95%), Query Frame = 0

Query: 564  GDFNVTRWIHERIPVSRATRGMRQFNKLINELGLLEL-----PLSNGKFTWSRPGDDSSQ 623
            GDFN      +R    +  R   +  +++ ++ L ++     P + G   +S P    + 
Sbjct: 151  GDFNTPLSSKDRSWKQKLNRDTVKLTEVMKQMDLTDIYRTFYPKTKGYTFFSAP--HGTF 210

Query: 624  SLIDRFLISKEWDVMFDNSRVSKQVRTISDHFPLLLEAGNFVWGPSP---FRVFNSWLN- 683
            S ID  +  K     + N  +   +  +SDH  L L   N +    P   +++ N+ LN 
Sbjct: 211  SKIDHIIGHKTGLNRYKNIEIVPCI--LSDHHGLRLIFNNNINNGKPTFTWKLNNTLLND 270

Query: 684  --MADCIK-----IVELTLSQDKSYGWVGFVIASKLRKLKINIKNWFAVFERERKQKEKS 743
              + + IK      +E   ++  +Y        +    +K  ++         +K++E +
Sbjct: 271  TLVKEGIKKEIKDFLEFNENEATTY-------PNLWDTMKAFLRGKLIALSASKKKRETA 330

Query: 744  LLDEIAWFDAKAEDNQLSSEEISLRTFVRSELLDLYLVEERNSIQK---CKLLWLKAGDE 803
                +       E  + +S + S R  +     ++  VE R +IQ+    +  + +  ++
Sbjct: 331  HTSSLTTHLKALEKKEANSPKRSRRQEIIKLRGEINQVETRRTIQRINQTRSWFFEKINK 390

Query: 804  NTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIEFEVLGFFTKLY----QALPEKRVF 863
                  R     + K+LI  + +  G       EI+  +  F+ +LY    + L E   F
Sbjct: 391  IDKPLARLTKGHRDKILINKIRNEKGDITTDPEEIQNTIRSFYKRLYSTKLENLDEMDKF 450

Query: 864  PFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPGPEGFTSEFFIKFWEFLKADF 923
               +    ++Q+Q   L +P   +EI   + +L   K+PGP+GF++EF+  F E L    
Sbjct: 451  LDRYQVPKLNQDQVDHLNSPISPKEIEAVINSLPTKKSPGPDGFSAEFYQTFKEDLIPIL 510

Query: 924  IRLFSELHRNGHLNSCLKENFICLIQKEEVVLT-IKDFRPISLTSSVYKILAKVLAKRLK 983
             +LF ++   G L +   E  I LI K +   T I++FRPISL +   KIL K+LA R++
Sbjct: 511  HKLFHKIEVEGTLPNSFYEATITLIPKPQKDPTKIENFRPISLMNIDAKILNKILANRIQ 570

Query: 984  KVIPSIISPYQSAFVEGRQILDPILIANEAVEYY-RVKNKKGWILKLDIEKAFDCVDWDF 1043
            + I +II P Q  F+ G Q    I  +   + Y  ++K+K   I+ LD EKAFD +   F
Sbjct: 571  EHIKAIIHPDQVGFIPGMQGWFNIRKSINVIHYINKLKDKNHMIISLDAEKAFDKIQHPF 630

Query: 1044 LDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVASRGLRQGDPLSPFLFLLIS 1103
            + KVL   G +  ++  I+     P  ++ +NG     I    G RQG PLSP+LF ++ 
Sbjct: 631  MIKVLERSGIQGPYLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVL 690

Query: 1104 EVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCKDDDGMFNTLIQTIELFEW 1163
            EV   L   I  +   +G  +G+++V +S+L  ADD I++  D       L+  I  F  
Sbjct: 691  EV---LARAIRQQKEIKGIQIGKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGE 750

Query: 1164 CSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYLGLPLGGHPKKY--SFWQP 1223
              G KIN  KS       +              +      YLG+ L    K      ++ 
Sbjct: 751  VVGYKINSNKSMAFLYTKNKQAEKEIRETTPFSIVTNNIKYLGVTLTKEVKDLYDKNFKS 810

Query: 1224 VLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSL--FLLSSSISINLDRILRSF 1283
            +  ++++ + RWK +  S  GR+ +    +    +Y  +     + +     L+  +  F
Sbjct: 811  LKKEIKEDLRRWKDLPCSWIGRINIVKMAILPKAIYRFNAIPIKIPTQFFNELEGAICKF 870

Query: 1284 FWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGWRFMMEPH-SFWRRV 1314
             W   +       +  SL+ + + +GG+ +  L     A++ K  W +  +     W R+
Sbjct: 871  VWNNKKPR-----IAKSLLKDKRTSGGITMPDLKLYYRAIVIKTAWYWYRDRQVDQWNRI 909

BLAST of Lag0030702 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.4e-20
Identity = 63/170 (37.06%), Postives = 91/170 (53.53%), Query Frame = 0

Query: 1197 VLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLSSSISINLDRILRSFFW 1256
            +L++V  ++  W+   LS  GRLTL  +VLSS+P++ +S  LL  SI   LD++ R+F W
Sbjct: 16   ILERVSSRMSGWREKTLSFAGRLTLTKAVLSSMPVHSMSTILLPQSILNRLDQLSRTFLW 75

Query: 1257 EGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGWRFMMEPHSFWRRVIVN 1316
                  K  HLV WS V + +K GGLG+ A    N AL++K GWR + E +S W  V+  
Sbjct: 76   GSTAEKKKQHLVKWSKVCSPKKEGGLGVRAAKSMNRALISKVGWRLLQEKNSLWTLVLQK 135

Query: 1317 IYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSL-AHFKLGNGMKIRF 1366
             Y   +   +       S  S W SIA   +  VS    +  G+G +IRF
Sbjct: 136  KYHVGEIRDSRWLIPKGSWSSTWRSIAIGLRDVVSHGVGWIPGDGQQIRF 185

BLAST of Lag0030702 vs. ExPASy TrEMBL
Match: A0A438FWU5 (LINE-1 retrotransposable element ORF2 protein OS=Vitis vinifera OX=29760 GN=LORF2_70 PE=4 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 3.9e-191
Identity = 360/905 (39.78%), Postives = 503/905 (55.58%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            ETK  ++D+  + S+W  K V W  + + G  GG++ILWD SKL+  E + G        
Sbjct: 771  ETKRETWDRRFVSSVWKGKRVEWAALPACGASGGIVILWDSSKLECTEKVLGSFSVTVKF 830

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNV R I E++  +R
Sbjct: 831  NSGEEGSFWLTSVYGPINPLWRKDFWLELQDLYGLTFPRWCVGGDFNVIRRISEKLGETR 890

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             T  MR F++ I E GL++ PL N  FTWS    D     +DRFL S EWD  F  S   
Sbjct: 891  LTLNMRCFDEFIRESGLIDPPLRNAAFTWSNMQADPICKRLDRFLFSSEWDTFFSQSFQE 950

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
               R  SDH P+ LE     WGP+PFR  N WL   +  +   +   +    GW G    
Sbjct: 951  ALPRWTSDHSPICLETNPLKWGPTPFRFENMWLLHPEFKEKFRVWWLECTGEGWEGHKFM 1010

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ +K  +K W  +   + K+++K +L +++  D   ++  L+S+ +  RT  R EL 
Sbjct: 1011 RKLKFVKSKLKEWNIMTFGDLKERKKLILTDLSRIDLIEQEGNLNSDLVLERTLKRRELE 1070

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
            D+ L EE    QK ++ W+K GD N+ FFHR    ++ +  I  L S  G +L    +I 
Sbjct: 1071 DVLLKEEVQWRQKSRVKWIKEGDCNSKFFHRVATGRRSRKFIKSLISERGETLNNIEDIS 1130

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E++ FF  LY     +       DW  +S      L  PF  EE+   +  L K KAPG
Sbjct: 1131 EEIVNFFGNLYSKPVGESWRVEGIDWVPISGESGGWLDRPFTEEEVRRAVFQLNKEKAPG 1190

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GFT   + + W+ +K D +R+F E H NG +N      FI L+ K+   + I D+RPI
Sbjct: 1191 PDGFTIAVYQECWDVIKEDLMRVFLEFHTNGVINQSTNATFIALVPKKSQSVKISDYRPI 1250

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +S+YKI+AKVL+ RL+KV+   IS  Q AFVEGR ILD +LIANE V+  R   ++G
Sbjct: 1251 SLVTSLYKIIAKVLSGRLRKVLHETISDSQGAFVEGRHILDAVLIANEVVDEKRRSGEEG 1310

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
             + K+D EKA+D VDW FLD VL  KGF +KW  WI+GC+ +  F++ +NG  +G + AS
Sbjct: 1311 IVFKIDFEKAYDHVDWGFLDHVLQRKGFSQKWRLWIRGCLSSSSFAILVNGNAKGWVKAS 1370

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF L+++V S ++ +    G  EGF VG+D+  VS+LQFADDTI F K
Sbjct: 1371 RGLRQGDPLSPFLFTLVADVLSRMLFRAEETGLTEGFSVGRDRTRVSLLQFADDTIFFSK 1430

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
                    L   + +F   SGLKIN EKS + GIN     +   AS  +C+V   P +YL
Sbjct: 1431 ASMEHLQNLKIILLVFGQVSGLKINLEKSTISGINTRQELLSSLASVFDCRVSEWPLSYL 1490

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            GLPLGG+PK   FW PV++++ +++D WK+  LS GGR+TL  S LS IP YFLSLF + 
Sbjct: 1491 GLPLGGNPKTIGFWDPVVERISRRLDGWKKAYLSLGGRITLIQSCLSHIPSYFLSLFKIP 1550

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
            +SI+  ++++ R+F W G    K +HLV W +VS  ++ GGLG G ++ RN+ALL KW W
Sbjct: 1551 ASIASKIEKMQRNFLWSGAGEGKKDHLVRWEVVSRPKELGGLGFGKISLRNIALLGKWLW 1610

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            RF  E    W +VI +IYGT   GW++      S R PW +IA+++Q F       +GNG
Sbjct: 1611 RFPRERSGLWYKVIGSIYGTHPNGWDANMVVRWSHRCPWKAIAQVFQEFSPFVRLVVGNG 1670

BLAST of Lag0030702 vs. ExPASy TrEMBL
Match: A0A803QI00 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 8.6e-191
Identity = 363/905 (40.11%), Postives = 502/905 (55.47%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            E K  S D+  I SIW S+   W+ + + GR GG L++WD   + +++ L G        
Sbjct: 935  EVKRTSVDRRFIGSIWRSRFKAWIIIPAIGRSGGTLLIWDTRTITVLDSLVGEFSISVLI 994

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNVTR   E++  S 
Sbjct: 995  KAEGKDPWWFSGVYGPCSYKLRPAFWDELAGLSAICGDSWCVGGDFNVTRRPGEKLNSSS 1054

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             TR M+ F+ LI EL L++  L NG+FTWS        S +DRFL +  W+V++   R  
Sbjct: 1055 CTRSMKLFDGLIRELRLIDPKLENGRFTWSNFRTSPVCSRLDRFLFTNNWNVIYPFVRQE 1114

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
              VR +SDH P+++++    WGP PFR  N WL      K       +  S GW G    
Sbjct: 1115 MLVRLVSDHSPVVIDSNPPRWGPGPFRFDNQWLEHNSFPKSFGRWWKEASSNGWPGTKFM 1174

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
            SKL+K +  +K W +    + K  +++L   +   D     N      +  R  ++ E  
Sbjct: 1175 SKLKKTQEKVKEWSSSTFGQNKATKRALEGRLVALDRLEGTNSWVQSLVEERRKLKEEWQ 1234

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
             L   EER+   K K  W K GD N+ FFH  L A+K +  I+ +   DGS +    EI 
Sbjct: 1235 QLNFEEERSIWLKSKCKWAKEGDANSRFFHNLLNARKARNTISRIEREDGSIIDKEEEIV 1294

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E++GFF+KLY +   +     + +W  ++ +    L + F  EE+   + +   +KAPG
Sbjct: 1295 EELIGFFSKLYTSEARRGSGIESIEWQRIAYSSACQLESSFEEEEVKRSVFSCEGSKAPG 1354

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GF+   F   WE +K D + +F    + G +   + E FICLI K      +KDFRPI
Sbjct: 1355 PDGFSLAVFQNNWETIKDDLMEVFRTFEKEGRIEGSINETFICLIPKRLNSCKVKDFRPI 1414

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +SVYKI+AK LA RL+ V+   IS  QSAFVEGRQILD +LIANE VE +R + KKG
Sbjct: 1415 SLITSVYKIVAKTLATRLRGVLGETISETQSAFVEGRQILDSVLIANETVEDFRSRGKKG 1474

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
            ++ K+D+EKA+D VDWDFLD VL  KGF + W +WI+GCV +  FS+ INGR RG+   S
Sbjct: 1475 FVFKIDLEKAYDRVDWDFLDLVLKEKGFGEVWRKWIRGCVSSTSFSLLINGRVRGKFRGS 1534

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF L+ +V   LVDK      F GF VG+D + +S LQFADDT+ F K
Sbjct: 1535 RGLRQGDPLSPFLFTLVVDVLGRLVDKAAQSDTFSGFQVGKDNIQISHLQFADDTLFFVK 1594

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
            D+  +   L++ +E F   SGLK+N  KS L GI+L++  V   A  I C+V   P  YL
Sbjct: 1595 DEASL-RKLVEIVEAFCGISGLKVNLNKSQLLGISLEEEVVAQNAEIIGCEVGTWPMTYL 1654

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            G+PLGG P+K +FW+PVLDK  K++D WK   LSRGGRL L  SVLSS+P+Y+LSLF   
Sbjct: 1655 GMPLGGSPRKGTFWEPVLDKCAKRLDGWKCSFLSRGGRLILIQSVLSSLPIYYLSLFKAP 1714

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
              +   +++++R FFWEG + +  +HLV W  V   +  GGL IG L  RN  LL KW W
Sbjct: 1715 KMVLQAIEKMMRDFFWEGGDLAGGDHLVAWDEVCKPRSEGGLAIGRLEMRNKGLLMKWLW 1774

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            R+ +EP+S W +VI + YG +   W+++     S R PW  I+  +  +  L  FK+GNG
Sbjct: 1775 RYPLEPNSLWHKVIKSRYGKADNFWDTKWGARASPRGPWKDISDYYDEYGQLVKFKVGNG 1834

BLAST of Lag0030702 vs. ExPASy TrEMBL
Match: A0A803P8A0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 676.4 bits (1744), Expect = 2.5e-190
Identity = 361/905 (39.89%), Postives = 503/905 (55.58%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            E K  + D+  I SIW S+   W+ + + GR GG L++WD   + +++ L G        
Sbjct: 36   EVKRATVDRRFIGSIWRSRFKAWILLPAIGRSGGTLLIWDTRIISVLDSLVGEFSISVLI 95

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNVTR + E++  S 
Sbjct: 96   NAEGKEPWWFSGVYGPCSYKIRHVFWDELAGLSSICGESWCVGGDFNVTRRVGEKLNSSS 155

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
            +TR M+ F+ LI EL L++  L NG FTWS        S +DRFL    W+V+F   R  
Sbjct: 156  STRSMKLFDGLIRELQLIDPKLENGSFTWSNFRAIPICSRLDRFLFLNNWNVVFPFVRQE 215

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
              VR +SDH P+++++    WGP PFR  N WL      K  E    ++   GW G    
Sbjct: 216  MLVRLVSDHSPVVIDSKPPKWGPGPFRFDNHWLEHKSFSKCFESWWQEEIIDGWPGTKFM 275

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ L+   K W      + K  + +L   +   D +      +      R  ++ E  
Sbjct: 276  KKLKTLQGKAKEWSRFTYGQNKATKNALEGRLGVLDRQEGTPSWNQSLYDERRKLKEEWQ 335

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
             L   EER+   K K  W K GD N+ FFH  L A+K +  I+ +   +G  + +  EI 
Sbjct: 336  RLTFEEERSIWLKSKCKWAKEGDANSRFFHNLLNARKARNTISRIERDNGDIIDSEKEIV 395

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E++ FF+KLY +           +W  +++     L  PF  +E+   + +   +KAPG
Sbjct: 396  EELIAFFSKLYTSETRMGTGVEGIEWQHIAEPSARQLECPFEEDEVRNIVFSCEGSKAPG 455

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GF+   F   WE +K + + +F   H  G +   + + FICLI K      +KDFRPI
Sbjct: 456  PDGFSLAVFQNNWEVIKNELMEVFRAFHSEGRIEGSINDTFICLIPKRLNSCKVKDFRPI 515

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +SVYKI+AK LA RL+ V+   IS  QSAFVEGRQILD +L+ANEAVE YR + KKG
Sbjct: 516  SLITSVYKIIAKTLATRLRGVLGETISETQSAFVEGRQILDSVLLANEAVEDYRSRGKKG 575

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
            ++LK+D EKA+D VDW FLD VL  KGF ++W +WI+GCV +  FS+F+NGR RG+   S
Sbjct: 576  FVLKIDFEKAYDRVDWGFLDLVLRKKGFGERWRKWIRGCVSSTSFSIFVNGRVRGKFHGS 635

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF L+++V   +VDK     AF GF +G+D + +S LQFADDT+ F K
Sbjct: 636  RGLRQGDPLSPFLFTLVADVLGRMVDKAVETEAFSGFQIGKDNIRLSHLQFADDTLFFVK 695

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
            D+D +   L++ +E F   SGLK+N  KS L GI L D  V   A+ I C+V   P  YL
Sbjct: 696  DEDSL-QKLVKIVEAFCGISGLKVNLNKSQLLGICLSDEAVAQGANLIGCEVGKWPMTYL 755

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            G+PLGG P+K +FW+PVLDK  K++D WK   LSRGGRLTL  SVLSS+P+Y+LSLF + 
Sbjct: 756  GMPLGGSPRKKTFWEPVLDKCAKRMDGWKCSFLSRGGRLTLIQSVLSSLPIYYLSLFKVP 815

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
              +   L++++R FFWEG + +  +HLV W  V   +  GGL IG L  RN  LL KW W
Sbjct: 816  KMVLKELEKMMRDFFWEGGDLAGGDHLVAWDEVCKPRAEGGLAIGRLEMRNKGLLMKWLW 875

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            RF +E +S W +VI + YG +   W+++     S R PW+ IA ++  +  +  FK+GNG
Sbjct: 876  RFPLESNSLWHKVIKSRYGKADNFWDTKQGVRMSPRGPWMDIADLYHEYGKMVKFKVGNG 935

BLAST of Lag0030702 vs. ExPASy TrEMBL
Match: A0A803QEA6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 668.7 bits (1724), Expect = 5.2e-188
Identity = 356/905 (39.34%), Postives = 506/905 (55.91%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            E K  + D+  I SIW S+   W+ + + GR GG L++WD   + +++ L G        
Sbjct: 386  EVKRATVDRRFIGSIWRSRFKAWILIPAIGRSGGTLLIWDTRTISVLDSLVGEFSISVLI 445

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                       GDFNVTR + E++  S 
Sbjct: 446  NAEGKEPWWFSGVYGPCSYKLRPEFWDELAGLSSICGKSWCVAGDFNVTRRVGEKLNSSS 505

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             TR M+ F+ LI EL L++  L NG FTWS        S +DRFL +  W+++F   R  
Sbjct: 506  FTRSMKLFDGLIRELQLIDPKLENGSFTWSNFRASPVCSRLDRFLFTNNWNIIFPFVRQE 565

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
              VR +SDH P+++++    WGP PFR  N WL+     K  E    ++ + GW G    
Sbjct: 566  LLVRIVSDHSPVVIDSNPPKWGPGPFRFDNHWLDHKSFSKCFERWWKEEINDGWPGTKFM 625

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ L+  +K W      + + K+ +L   +   D     +  +   +  R  ++ E  
Sbjct: 626  KKLKILQGKVKEWSKSTFGQNRAKKIALEGRLGVLDKLEGTSFWNQSLLDERRKLKEEWK 685

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
             L   EER +  K K  W + GD N+ FFH  L A+K +  I+ +   +G  +    EI 
Sbjct: 686  WLNFEEERGTWLKSKCKWAREGDANSRFFHNLLNARKARNTISRIERENGDIIDNEKEIA 745

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E++ FF+KLY +           +W  ++++    L  PF  EE+   + +   NKAPG
Sbjct: 746  EELIAFFSKLYTSEARMGSGIEGIEWQQIAESSAGQLECPFEEEEVRNIVFSCEGNKAPG 805

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GF+       WE +K D + +F+  HR G +   + + FICLI K      +KDFRPI
Sbjct: 806  PDGFSLAVLQHNWETIKHDLMEVFTAFHREGRIEGSINDTFICLIPKRLNSCKVKDFRPI 865

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +SVYKI+AK LA RL+ V+   IS  QSAFVEGRQILD +L+ANEAVE YR + +KG
Sbjct: 866  SLITSVYKIIAKTLATRLRGVLGETISETQSAFVEGRQILDSVLMANEAVEDYRSRGRKG 925

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
            ++LK+D EKA+D VDW FLD VL  KGF ++W +WI+GCV +  FS+FINGR RG+   S
Sbjct: 926  FVLKIDFEKAYDRVDWGFLDMVLRKKGFGERWRKWIRGCVSSTSFSIFINGRVRGKFNGS 985

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQGDPLSPFLF +I++V   +VDK     +  GF +G+D + +S LQFADDT+ F K
Sbjct: 986  RGLRQGDPLSPFLFTMIADVLGRMVDKAIETESLTGFQIGKDDIRLSHLQFADDTLFFVK 1045

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
            D+  +   L++ ++ F   SGLK+N  KS L GI +++  V   A  I C+V   P  YL
Sbjct: 1046 DEVSL-QKLVKVVKAFCGISGLKVNLNKSQLLGICMNEEAVAQSAILIGCEVGRWPMTYL 1105

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            G+ LGG P+K SFW+PVLDK  K++D WK   LSRGGRLTL  SVLSS+P+Y+LSLF   
Sbjct: 1106 GMSLGGSPRKRSFWEPVLDKCAKRMDGWKCSFLSRGGRLTLIQSVLSSLPIYYLSLFKAP 1165

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
              +   L++++R FFWEG + +  +HLV W  V   +  GGL IG L+ RN  LL KW W
Sbjct: 1166 KVVLKELEKMMREFFWEGGDLAGGDHLVAWDEVCKPRAEGGLAIGKLDMRNKGLLMKWLW 1225

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            RF +EP+S W +VI + YG +   W+++     S R PW  I+ ++  +  L  FK+GNG
Sbjct: 1226 RFPLEPNSLWHKVIKSRYGKADNFWDTKQGVRISPRGPWKDISDLYDEYGKLVKFKVGNG 1285

BLAST of Lag0030702 vs. ExPASy TrEMBL
Match: A0A803QQM3 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 8.9e-188
Identity = 358/905 (39.56%), Postives = 498/905 (55.03%), Query Frame = 0

Query: 511  ETKMVSFDQCLIKSIWSSKDVGWVNVKSWGRLGGLLILWDESKLKIVEFLQG-------- 570
            E K  + D+  I SIW S+   W+ + + GR GG L++WD   + +++ L G        
Sbjct: 959  EVKRATVDRRFIGSIWRSRFKAWILLPALGRSGGTLLIWDTRTISVLDSLVGEFSISVLI 1018

Query: 571  ------------------------------------------GGDFNVTRWIHERIPVSR 630
                                                      GGDFNVTR + E++  S 
Sbjct: 1019 NAEGKEPWWFSGVYGPCSYKLRPEFWDELAGLSSICGESWCVGGDFNVTRRVGEKLNSSS 1078

Query: 631  ATRGMRQFNKLINELGLLELPLSNGKFTWSRPGDDSSQSLIDRFLISKEWDVMFDNSRVS 690
             TR M+ F+ LI EL L++  L NG FTWS        S +DRFL S  W+V++   R  
Sbjct: 1079 CTRSMKLFDGLIRELQLIDPKLENGSFTWSNFRASPVCSRLDRFLFSNNWNVIYPFVRQE 1138

Query: 691  KQVRTISDHFPLLLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKSYGWVGFVIA 750
              VR +SDH P+++++    WGP PFR  N WL      K  E    ++ + GW G    
Sbjct: 1139 MLVRLVSDHSPVVIDSNPPKWGPGPFRFDNHWLEHKSFSKCFESWWKEEINDGWPGTKFM 1198

Query: 751  SKLRKLKINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLRTFVRSELL 810
             KL+ L+  +K W      + K  + +L   +   D     +  +   +  R  ++ E  
Sbjct: 1199 KKLKLLQGKVKEWSKSTFGQNKATKIALEGRLGVLDRLEGTSSWNQSVLDERRKLKEEWQ 1258

Query: 811  DLYLVEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIE 870
             L+  EER    K K  W + GD N+  FH  L A+K K  I+ +   +G  +    EI 
Sbjct: 1259 QLHFEEERGIWLKSKCKWAREGDANSRLFHNLLNARKAKNTISRIERDNGDIIDNEKEIV 1318

Query: 871  FEVLGFFTKLYQALPEKRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLGKNKAPG 930
             E++ FF+KLY +           +W  + ++    L  PF  EE+   + +   NKAPG
Sbjct: 1319 EELIAFFSKLYTSEARSGTGIEGIEWHKIEESSARQLECPFEEEEVRNIVFSCEGNKAPG 1378

Query: 931  PEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTIKDFRPI 990
            P+GF+       WE +K D + +F   HR G +   + + FICLI K      +KD+RPI
Sbjct: 1379 PDGFSLAALQNNWETIKYDLMEVFRAFHREGRIEGSINDTFICLIPKRLNSCKVKDYRPI 1438

Query: 991  SLTSSVYKILAKVLAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAVEYYRVKNKKG 1050
            SL +SVYKI+AK LA RL+ V+   IS  QSAFVEGRQILD +L+ANEAVE YR + KKG
Sbjct: 1439 SLITSVYKIIAKTLATRLRGVLGETISETQSAFVEGRQILDSVLMANEAVEDYRSRGKKG 1498

Query: 1051 WILKLDIEKAFDCVDWDFLDKVLCFKGFEKKWIQWIQGCVRNPKFSVFINGRPRGRIVAS 1110
             +LK+D EKA+D VDW FLD V+  KGF ++W +WI+GCV    FS+FINGR RG+   S
Sbjct: 1499 IVLKIDFEKAYDRVDWGFLDLVMRKKGFGERWTKWIRGCVSTTSFSIFINGRVRGKFNGS 1558

Query: 1111 RGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSILQFADDTILFCK 1170
            RGLRQ DPLSPFLF LI++V   +VDK     +  GF +G+D + +S LQFADDT+ F K
Sbjct: 1559 RGLRQVDPLSPFLFTLIADVLGRMVDKAIDTESLSGFQIGKDDIQLSHLQFADDTLFFVK 1618

Query: 1171 DDDGMFNTLIQTIELFEWCSGLKINWEKSALCGINLDDAKVCHFASRINCKVEVLPFNYL 1230
            D+  +   L++ +E F   SGLK+N  KS L G+ +D+  V   A +I C+V   P  YL
Sbjct: 1619 DEASL-QKLVKIVEAFCGISGLKVNLNKSQLLGVCMDEDAVAQSAIQIGCEVGRWPMTYL 1678

Query: 1231 GLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFLSLFLLS 1290
            G+PLGG P+K SFW+PVLDK   ++D WK   LSRGGRLTL  SVLSS+P+YFLSLF   
Sbjct: 1679 GMPLGGSPRKRSFWEPVLDKCATRMDGWKCSFLSRGGRLTLIQSVLSSLPIYFLSLFKAP 1738

Query: 1291 SSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNMALLAKWGW 1350
              +   L++++R FFWEG + +  +HLV W  V   +  GGL IG L  RN  LL KW W
Sbjct: 1739 KVVLKELEKMMRDFFWEGGDLAGGDHLVAWDEVCKPRAEGGLAIGRLEMRNKGLLMKWLW 1798

Query: 1351 RFMMEPHSFWRRVIVNIYGTSKFGWNSENRTCCSLRSPWLSIAKIWQRFVSLAHFKLGNG 1366
            RF +E +S W +VI + YG +   W++++    S R PW  I+ ++  +  L  FK+GNG
Sbjct: 1799 RFPLESNSLWHKVIKSRYGRADNFWDTKHGVRLSPRGPWKDISDLYDEYGKLVKFKVGNG 1858

BLAST of Lag0030702 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 119.0 bits (297), Expect = 3.0e-26
Identity = 110/436 (25.23%), Postives = 193/436 (44.27%), Query Frame = 0

Query: 542 LGGLLILWDESKLKIV-----EFLQGGGDFN---VTRWIHERIPVSRATRGMRQFNKLIN 601
           LG + I+WD S   +V     + +   GDF+    T   +  +  S   RG+ +F   + 
Sbjct: 198 LGRIWIVWDPSVSVLVFKKTDQLMILVGDFDQIAATSDHYSVLQTSIPMRGLEEFQNCLR 257

Query: 602 ELGLLELPLSNGKFTWSRPGDDSS-QSLIDRFLISKEWDVMFDNSRVSKQVRTISDHFPL 661
           +  L+++P     +TWS   DD+     +DR + + +W   F ++    ++  +SDH P 
Sbjct: 258 DSDLVDIPSRGVHYTWSNHQDDNPIIRKLDRAIANGDWFSSFPSAIAVFELSGVSDHSPC 317

Query: 662 LLEAGNFVWGPSPFRVFNSWLNMADCIKIVELTLSQDKS--YGWVGFVIASKLRK----L 721
           ++   N          + S+L+      +V LT++ ++    G   F +   L+      
Sbjct: 318 IIILENLPKRSKKCFRYFSFLSTHPTF-LVSLTVAWEEQIPVGSHMFSLGEHLKAAKKCC 377

Query: 722 KINIKNWFAVFERERKQKEKSLLDEIAWFDAKAEDNQLSSEEISLR--TFVRSELLDLYL 781
           K+  +  F   + + K+   SL    +       D+    E ++ +   F  + L   Y 
Sbjct: 378 KLLNRQGFGNIQHKTKEALDSLESIQSQLLTNPSDSLFRVEHVARKKWNFFAAALESFYR 437

Query: 782 VEERNSIQKCKLLWLKAGDENTNFFHRFLAAKKRKLLITGLNSIDGSSLLTAGEIEFEVL 841
                  QK ++ WL+ GD NT FFH+ + A + K LI  L   D   +    +++  ++
Sbjct: 438 -------QKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMDDDVRVENVTQVKEMIV 497

Query: 842 GFFTKLYQA-----LPE-----KRVFPFNFDWSMVSQNQNSALIAPFFVEEIWLPLKNLG 901
            ++T L  +      P+     K + PF  + ++ S  + SAL +    +EI   +  + 
Sbjct: 498 AYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLAS--RLSALPSD---KEITAAVFAMP 557

Query: 902 KNKAPGPEGFTSEFFIKFWEFLKADFIRLFSELHRNGHLNSCLKENFICLIQKEEVVLTI 951
           +NKAPGP+ FT+EFF + W  +K   I    E  R GHL        I LI K   V  +
Sbjct: 558 RNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAITLIPKVTGVDQL 617

BLAST of Lag0030702 vs. TAIR 10
Match: AT3G24255.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 79.3 bits (194), Expect = 2.6e-14
Identity = 48/140 (34.29%), Postives = 72/140 (51.43%), Query Frame = 0

Query: 1175 LPFNYLGLPLGGHPKKYSFWQPVLDKVQKKIDRWKRINLSRGGRLTLCSSVLSSIPLYFL 1234
            LP  YLGLPL       S + P+++K++ +I +W   +LS  GRL L SSV+ S+  +++
Sbjct: 23   LPVRYLGLPLLTKKMTTSDYGPLVEKIRVRIGKWTARHLSFAGRLQLISSVIHSLTNFWM 82

Query: 1235 SLFLLSSSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGALNQRNM-- 1294
            S F L S+    +D I  SF W G E +     V WS V   +  GGLGI +L + N   
Sbjct: 83   SAFRLPSACIKEIDSICSSFLWSGPELNTKKAKVAWSDVCTPKDEGGLGIRSLKEANKGS 142

Query: 1295 -------ALLAKWGWRFMME 1306
                     L  W W+ +++
Sbjct: 143  FWSISGNTTLGSWMWKKILK 162

BLAST of Lag0030702 vs. TAIR 10
Match: ATMG01250.1 (RNA-directed DNA polymerase (reverse transcriptase) )

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-11
Identity = 33/67 (49.25%), Postives = 43/67 (64.18%), Query Frame = 0

Query: 1049 INGRPRGRIVASRGLRQGDPLSPFLFLLISEVFSALVDKIHLKGAFEGFLVGQDKVHVSI 1108
            ING P+G +  SRGLRQGDPLSP+LF+L +EV S L  +   +G   G  V  +   ++ 
Sbjct: 14   INGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRINH 73

Query: 1109 LQFADDT 1116
            L FADDT
Sbjct: 74   LLFADDT 80

BLAST of Lag0030702 vs. TAIR 10
Match: AT4G20520.1 (RNA binding;RNA-directed DNA polymerases )

HSP 1 Score: 68.9 bits (167), Expect = 3.5e-11
Identity = 34/82 (41.46%), Postives = 52/82 (63.41%), Query Frame = 0

Query: 954  LAKRLKKVIPSIISPYQSAFVEGRQILDPILIANEAV-EYYRVKNKKGW-ILKLDIEKAF 1013
            + +RLK ++ ++I P Q++F+ GR   D I+   EAV    R K  KGW +LKLD+EKA+
Sbjct: 1    MVERLKPLMTNLIGPAQASFIPGRVSTDNIVFVQEAVHSMRRKKGVKGWMLLKLDLEKAY 60

Query: 1014 DCVDWDFLDKVLCFKGFEKKWI 1034
            D + WD+L+  L   GF + W+
Sbjct: 61   DRIRWDYLEDTLISAGFPEVWL 82

BLAST of Lag0030702 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 52.8 bits (125), Expect = 2.6e-06
Identity = 26/91 (28.57%), Postives = 45/91 (49.45%), Query Frame = 0

Query: 1228 SIPLYFLSLFLLSSSISINLDRILRSFFWEGNEGSKVNHLVGWSLVSNSQKNGGLGIGAL 1287
            ++P Y ++ FLL  ++   +  +L  F+W   + +K  H   W  +S  +  GG+G   +
Sbjct: 2    ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 1288 NQRNMALLAKWGWRFMMEPHSFWRRVIVNIY 1319
               N+ALL K  WR +  P S   +V  + Y
Sbjct: 62   EAFNLALLGKQMWRMLSRPESLMAKVFKSRY 92