Lag0029815 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0029815
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionTranscription initiation factor IIF subunit beta
Locationchr8: 42369909 .. 42375366 (-)
RNA-Seq ExpressionLag0029815
SyntenyLag0029815
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACGTCGACGACAAATCCACCGACCGTTTCAGTTCCAGAAAACTCCAAAGCAATGCCAACGTCGTCGTCGGAGCCGACTTCGTAGAGACGAACAAGGCGGACAGAGCAATGTGGCTCTTGAAATGCCCGCAAGCCGTAACTCGCGCCCTTTCCAATTCCTCCGATGGCCCCTCTCGTGCGGTCGCTAAGGTCATCGTTTCTGTTGACCCTCTGCAATCCAATGACGATGATGATTCTTCATCAACTGAGGTATATAATGACGTTCTGCTATGGCGCTTTTCGTTTTGGATTGAGGGCTCTTTTCTTATTGCATTGTGTGATTTATTGTGTTTCTTACTCGCTTGTGCATGCTTTCCATTTTAGGTTGCCTTGTTCGTTTCTTCTTCGTTTCGTATTGTCTTTTTCTTAGGGCTTTCTGTTTGTTTCTGTGCATGCATCTGCCCTTCTTTTAGTTTATGATTTGTTTCCGTTGGAGTTATTCATTGATTACTCGGTCTTTGCTTTTATCTTTTTGTTCCTTTCTTGTTTCACTGGCAACGGAAATTTTTGTTGTTTGTAAAAGTTTCAATGACGTGTGGTATCCTAAATTGCTTTTTCACTGTTTCGTTCTCGTGATGCTTTTCTGTGAATAAGACCCTTCTGGTTTACATGCTTGGTTTCCTAGCTACCAAATGTATATGGGTAGGACCGCTGGTTTTCAGAACACGGGGATCAGGAATTTATGGAGATTGGAAAAGGCCAAAGTAATTTAATGGTAGTGTTTTATCACTCTCTACTGACTAAAGGATATGTACAGGATGTTGTTAGTTATCACTATCAGTCCCCATTTTCTCATGTTTAACCCCGCCATGAGCCTATGACTACATGGTTCGGGTTCTTACTTTCGGTCACGAATCTTCGCTGTCCTTTCTTCCATGTTTTTCATTGGAGTTCTGGATGTTGCATAGATTGTTCAACTTTATGTCCATGATGCCCGGTGATGAGGCATGGGGTCCAATGTTCCAATTTAAAATCTTCAAATGTTTCATGGCCTCTTCCTTCTTTTCTGAAAGTTATTCATTGAGAAATTGCTGATTCATTGTGTAAAAAATCTGTAAAACTAAAGGGAGTGTTTTTTTTTTTTTATAGATTTGATTCTTATTGCAACAACAACTGGGTTGGTGGTTTCTGCATTACAAGGCCAATTATCTGGTTTCTTTCTTTAAAATGGGACTCTCTGGGTGTTTGAATTGAAGGGCATTTGTTTAGAAGATAGGTATTTAATAGATGGAAAAGCTGGGAGTAGGCTTGTTCAGCTTTTGTGTCTAAGCTTATAAATTTGAAAAATCTTTGAGGATATGTTTGCTGAACCTGGTATTCTGTGCGATAAAATTGTTAAAATGGTTCGTCTTTTTTGGCTGGAGAACTATCTGGATATTGTATACCATCTTAATATTCTGATTGAACGAGTAACTGGCATAAATTTGGTTTTTCATTTAACACTTTGAGTTGGTCTAGTAAAAGGAGAAGTTAAAAAAAAATAAAGTGACTTTGGATTAGGGGTTCAAATCCACCTATTTATTTTATTTAAGTTTTACAAGTTTTCTTTCAACTAAATGTTATAGACTTGTGATTGTTCCATAGAGATGTTTGTAAGTTGACTTGGTTGCCTTAGTATCAAGAAAAAATATCTAAAAGGAGTAACAATAGAGTCTTTAGATTGTGAAATGCTATGGCCCCGTTTGATAACCATATTTTCTCAAACTGTCACTGTTTAGTGTTTATTTATAGCTTTGAAATGATATAAGAATTTCTTGCTGTCATCTGAAAGGAAACAAATTGTAGTTGCCCTCTGGTCTATTAGCACGTTAAATAAGATGCTCTGTGTAATAGTTGTGACTTGTAAATGGTTTTAGCTAAAAATTACAAGACCTTTGGTTTGGCCACATCCAAGGAGGCTCGGGAACCTTTTAGGGAGAAAGTATATTTTTCATCACAATCTAGAAGATGTAACCACAATATTATGATTGATTGCCAGCCGTGTCATGTGAATGATCTTCTCTTTGCTTCTAAATTATTTTCACCTGCATTTAAGGCACATTTCCAAGATAGTTCTTAGTTATTTCTCCTTTAATAAATTATGCAGTTCTTTGTCATTGAAGATGAATGCTTTATTCTTGAAGTGAGAACAAATTCATGTGTTGCTGAGAGTACAATTAATTTTGTACAATTTGATTAAATAAAGAACAACATCTACCTGTATTTGGAATATTTGTCTGACATTAGACGAGTTTAAACATGAATGTCTTAGTATATAATCTTCAAGGTAGAAGTTTCACAATTTCATGATACGGACTTTGGCCTTCCAATTCTGATACTATCTTTTACTACTCCAATTATCTCATCTGAATCTTCTAAAGAATATTTCCTCATATAGCGCTCTTGTAACAGTTCTATTATAAGTAGTTTGTGCCTTACAGATTGTCAATTTACTCACCTTTTTAACCCTTCTCGGATTTTCTTACTTGCAAATGCAAGAAACGTTATCAGTTATTACCAAGTTGGACCTTATTTTTTTCAAGCATGGCTTCTTACATTGGTTATTATTTATACTAAAGGAGAAATTAAAGATTAATTATATCAACATCCATTTGCTTGCTTTCTTCGGCTAACCATGATTCTATAATTTTAGTTCACTATGGAATTGTCTGGCACTGACTCTGGAAATGCATTAAGGTCCTACTCGTTGAACATGTCCACGGACTTCATTCCGATGTCTGTATTTTCTGAGTCAACACAAGGTTGGTTTATGTCACTCTAGTCTGCTTATTAAAAAGAATATGATGCTTTCAAATGTTTGCTTGTAAGCTGGATGATCTATTACTCTAATTGTGATATAAGATCCAATTTATATAATGTGTTCAACTGGTACTGACAACTCCACGAATCTTGGATGTGAATATCTATTTTTATTTACTTTAGACCATATGTGCATTAATAATTAATTTCAGAATCTGTCTCATTCAATATTAATTTTATAGTAGTAACATCAATGAGTTTGATCGTCAACAATGCTTGTTTTGTTGCTATTATTCCTTTCTAATAGATTTGAATTAGGAAGGTTTTTAAAATATCTAGGTTTGGGCATCAGAGATATCTTTATACGCATCTTGCTGGTTATTTATACAGTGTGTATTGTTAGAAAAAGAAAAAGAAAATCTTACAATGTGGGAGTTAGTATGAGAGACAGTGTGTTCGGTTGAACCTGACTCCAATGAGTTCATTAGGCTTATGTTGTTATTCCTATTTCCTAATTAGGTGAGATGGAGCTTGTGTGCTGACATTGCCTTCTAAGTTCCCTCCCTTAATTGAGTCATAGTATCGAAAACTTCTATTCAAATTCTGCCAGTGTGATCTTTCATCTTAAAATTTTCTAAAACAAATAAAAATGACTAGTTCTGTGTTCTAGAGAGACTTCAAGAAATCTAGAGCAGTTATAACTTCTATTTTTCATATTGAAAATTTCTAATTCTTTACAGGACAAGCACATTTTTTTTTAAAAAAATATTGAACCTCTTAAATGTTCTTTTAGATCATTACTACACAATATCTGTCTAATGGTATCGACTCTTCTTTCATTTTAGGCAAGTTTACAGTCGAAGGGAAAATACTGAACAAATTTGACATGAAGCCGCATGATCAAAATCTTGAGAGGTACGGGAAACTCTGTCGCGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTCTCCGTTCTAACTTCACATGCGACGGTCTTGGGTGTCTGGTTACTTTATTTATTTCTTTCACGATCTTCTAATTCAAATTTCCAAATATTCCATGCAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCAATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGTAATTATACTACCTTCTTTTGTACATTGTTTGATATATTCTCATTATCAAGTTAAATTTTGATTTCGCTTGCTTATTAGGTTTAAGCCTGTGCTATATAATCTACCCTCGTAGTCAGTCCGAAGCTTGTCCAAATGCTTCCAAGAATTATTTTTTACTATTGTGAACATATGTGGTACTAAATTTGGTCTTCTAAAAAATGCGGTGCTAAATTTGGTACAGGAGAAGAAGAAAGTGGTAAGCAAGGGATCGGAAACGAAGAGGTTGAGGAAGGAGCGTGGTGAATTGGAGAAGATCATTTTTAGGCTCTTTGAAAGACAAGCATATTGGACGTCAAAACAATTGATTCAAGAGACTGACCAACCTGAAGTAAGATTTATCTTGAATTTGCACACCTATCGAAAACTAAGTTTGCACGTATAGTTAATCATCTGATTTTTCCCTCTCATCGAAAAAAATCATTTGGTTTTTCCCCGTGCATCAGTTCCGAGCAAAGTTTATATAACATTAACATGACTGCACATGGTAAATACAAATATAGATAAATCTGTCCAGAATTGATTCAAGACAATAGATAAACAATAAACAGTTGATTAGAAGACAACCCTATTGAACGTAAGATAATCATATGATAAGGGAGTGTTTGGAATGACTTTCGAAGTGCCTACAACAGTTTTTAAGCACTTAGAAAGTCATTCTAAACAACTATAAGTTCATTTTTAGACTCTTTGCAAAACAACCATATTAAAATTTATCCTGAGTCGCACACCTATCGAATACGAAGTTCACATTAGTGCATCAAATCACATGGTTTTTCCCTGTGAAACCACATGACTGCATATGTAAATACAGCTATAGATAAATCTGTCAACTATGGTATCAGTATTAGTATTATGCCCATTAGCTTTTAGACTTGCGACTGAAGTTGTACATTGTGATCGACTTGAAGGTAATTCTGGTATATAATCTCTTTTTTTTTTTTTCTTTCCTTTCTTTTCGCGTTCTCTTGAATACAAAATTAAATCGTACTTCATATCCACTTTCCTATTTTTCATTTCGAATAAGGTCACATATCTCATGGTTTTTCCCTGTTCTGGAATATGAAAGTAAATGGTACTTTATATCCACTTTTCTATTTAACGGTTGGCCTAGTGGTCAACAAAGGCCATGGAAAAAGTAGCAAGGTTAGATGAGGTGCGTGCAAGCTAGCCTGAATACTATATATATATATATATATATATATATATATATATTTCATTTAGGATAAGGTCACATATCTCTCAGACATCTGCTTCCTTAGGAAAGAAGCAAATGGACTACTCTCTGCAATTCTAGTTGATAGCTCTATTCTGCTTGTTGTTTAGGCTGAAACATCCAGGGCCTATCGTAAAAATCTTTCTTTTTTGCAGCAATATATGAAAGAAATCCTTAAAGACCTTTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCGGGCGAGGAAACGAAACCGCGATAG

mRNA sequence

ATGGACGTCGACGACAAATCCACCGACCGTTTCAGTTCCAGAAAACTCCAAAGCAATGCCAACGTCGTCGTCGGAGCCGACTTCGTAGAGACGAACAAGGCGGACAGAGCAATGTGGCTCTTGAAATGCCCGCAAGCCGTAACTCGCGCCCTTTCCAATTCCTCCGATGGCCCCTCTCGTGCGGTCGCTAAGGTCATCGTTTCTGTTGACCCTCTGCAATCCAATGACGATGATGATTCTTCATCAACTGAGGACCGCTGGTTTTCAGAACACGGGGATCAGGAATTTATGGAGATTGGAAAAGGCCAAAGTAATTTAATGTTCACTATGGAATTGTCTGGCACTGACTCTGGAAATGCATTAAGGTCCTACTCGTTGAACATGTCCACGGACTTCATTCCGATGTCTGTATTTTCTGAGTCAACACAAGGCAAGTTTACAGTCGAAGGGAAAATACTGAACAAATTTGACATGAAGCCGCATGATCAAAATCTTGAGAGGTACGGGAAACTCTGTCGCGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCAATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAAGTGGTAAGCAAGGGATCGGAAACGAAGAGGTTGAGGAAGGAGCGTGGTGAATTGGAGAAGATCATTTTTAGGCTCTTTGAAAGACAAGCATATTGGACGTCAAAACAATTGATTCAAGAGACTGACCAACCTGAAGCTGAAACATCCAGGGCCTATCGTAAAAATCTTTCTTTTTTGCAGCAATATATGAAAGAAATCCTTAAAGACCTTTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCGGGCGAGGAAACGAAACCGCGATAG

Coding sequence (CDS)

ATGGACGTCGACGACAAATCCACCGACCGTTTCAGTTCCAGAAAACTCCAAAGCAATGCCAACGTCGTCGTCGGAGCCGACTTCGTAGAGACGAACAAGGCGGACAGAGCAATGTGGCTCTTGAAATGCCCGCAAGCCGTAACTCGCGCCCTTTCCAATTCCTCCGATGGCCCCTCTCGTGCGGTCGCTAAGGTCATCGTTTCTGTTGACCCTCTGCAATCCAATGACGATGATGATTCTTCATCAACTGAGGACCGCTGGTTTTCAGAACACGGGGATCAGGAATTTATGGAGATTGGAAAAGGCCAAAGTAATTTAATGTTCACTATGGAATTGTCTGGCACTGACTCTGGAAATGCATTAAGGTCCTACTCGTTGAACATGTCCACGGACTTCATTCCGATGTCTGTATTTTCTGAGTCAACACAAGGCAAGTTTACAGTCGAAGGGAAAATACTGAACAAATTTGACATGAAGCCGCATGATCAAAATCTTGAGAGGTACGGGAAACTCTGTCGCGAAAGAACTCACAAGTCTATGACCAAAAGCAGACAGATTCAGGTGATTGATCATGTCACAGGAGGACATATGAGGCCAATGCCAGGGATGGACGTGTTGTCATTTGGAGCTGCAGAGAAGAAGAAAGTGGTAAGCAAGGGATCGGAAACGAAGAGGTTGAGGAAGGAGCGTGGTGAATTGGAGAAGATCATTTTTAGGCTCTTTGAAAGACAAGCATATTGGACGTCAAAACAATTGATTCAAGAGACTGACCAACCTGAAGCTGAAACATCCAGGGCCTATCGTAAAAATCTTTCTTTTTTGCAGCAATATATGAAAGAAATCCTTAAAGACCTTTGTGTCTACAACAACAAGGGTGTTCACCAAGGAACATATGAGCTGAAGCCAGAATACAAGGAATCGGGCGAGGAAACGAAACCGCGATAG

Protein sequence

MDVDDKSTDRFSSRKLQSNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPSRAVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGNALRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEETKPR
Homology
BLAST of Lag0029815 vs. NCBI nr
Match: XP_038889594.1 (general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_038889595.1 general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_038889596.1 general transcription factor IIF subunit 2-like [Benincasa hispida])

HSP 1 Score: 487.3 bits (1253), Expect = 1.0e-133
Identity = 259/313 (82.75%), Postives = 263/313 (84.03%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQSNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPSR 60
           MDVD KS DR SSRKLQ N N VVG DF+ETNKADRAMWLLKCPQ VTRALSNS DGPSR
Sbjct: 1   MDVDGKSIDRCSSRKLQINNNSVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDGPSR 60

Query: 61  AVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGNA 120
            VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+GTDSGN 
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELAGTDSGNT 120

Query: 121 LRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSM 180
           LRSYSLNMSTDFIPMSVFSES+QGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSM
Sbjct: 121 LRSYSLNMSTDFIPMSVFSESSQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSM 180

Query: 181 TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRL 240
           TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRL
Sbjct: 181 TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRL 240

Query: 241 FERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYEL 300
           FERQ+YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTYEL
Sbjct: 241 FERQSYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTYEL 275

Query: 301 KPEYKESGEETKP 314
           KPEYKESGEE KP
Sbjct: 301 KPEYKESGEEAKP 275

BLAST of Lag0029815 vs. NCBI nr
Match: XP_022133483.1 (general transcription factor IIF subunit 2-like [Momordica charantia])

HSP 1 Score: 478.4 bits (1230), Expect = 4.7e-131
Identity = 257/316 (81.33%), Postives = 262/316 (82.91%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQSNANVV--VGADFVETNKADRAMWLLKCPQAVTRALSNSSDGP 60
           MDVDDK  DR SSRKLQ+N N V   G DF+ETNKADRAMWLLKCPQAVTRALSNS DGP
Sbjct: 1   MDVDDKPIDRCSSRKLQTNNNSVSGTGTDFLETNKADRAMWLLKCPQAVTRALSNSPDGP 60

Query: 61  SRAVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSG 120
           SR VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+GTDSG
Sbjct: 61  SRPVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELAGTDSG 120

Query: 121 NALRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHK 180
           N+LRSYSLNMSTDFIPMSVFSES  GKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHK
Sbjct: 121 NSLRSYSLNMSTDFIPMSVFSESPPGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHK 180

Query: 181 SMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIF 240
           SMTKSRQIQVIDHVTGGHMRPMPGMDV SFGAAEKKKVVSKGSETKRLRKERGELEKIIF
Sbjct: 181 SMTKSRQIQVIDHVTGGHMRPMPGMDVTSFGAAEKKKVVSKGSETKRLRKERGELEKIIF 240

Query: 241 RLFERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTY 300
           RLFERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTY
Sbjct: 241 RLFERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTY 278

Query: 301 ELKPEYKESGEETKPR 315
           ELKPEYKESG+ETK R
Sbjct: 301 ELKPEYKESGDETKSR 278

BLAST of Lag0029815 vs. NCBI nr
Match: XP_008465665.1 (PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo])

HSP 1 Score: 477.2 bits (1227), Expect = 1.1e-130
Identity = 255/315 (80.95%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQ-SNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPS 60
           MDVD KS DR +SRK Q +N N+VVG DF+ETNKADRAMWLLKCPQ VTRALSNS D PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNNIVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDAPS 60

Query: 61  RAVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGN 120
           R VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+ TDSGN
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELASTDSGN 120

Query: 121 ALRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKS 180
           ALRSY+LNMSTDFIPMSVFSES+QGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKS
Sbjct: 121 ALRSYTLNMSTDFIPMSVFSESSQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKS 180

Query: 181 MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFR 240
           MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIF+
Sbjct: 181 MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFK 240

Query: 241 LFERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYE 300
           LFERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTYE
Sbjct: 241 LFERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTYE 277

Query: 301 LKPEYKESGEETKPR 315
           LKPEYKES EETKPR
Sbjct: 301 LKPEYKESSEETKPR 277

BLAST of Lag0029815 vs. NCBI nr
Match: XP_004143805.1 (general transcription factor IIF subunit 2 isoform X3 [Cucumis sativus] >KGN51637.2 hypothetical protein Csa_008814 [Cucumis sativus])

HSP 1 Score: 475.3 bits (1222), Expect = 4.0e-130
Identity = 253/315 (80.32%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQSNAN-VVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPS 60
           MDVD KS DR +SRK Q+N N +VVG DF+ETNKADRAMWLLKCPQ VTRALSNS D PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNSIVVGTDFLETNKADRAMWLLKCPQLVTRALSNSPDAPS 60

Query: 61  RAVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGN 120
           R VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+ TDSGN
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELASTDSGN 120

Query: 121 ALRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKS 180
           ALRSYSLNMSTDFIPMSVFSES+QGKFT+EGKILNKFDMKPHDQNLERYGKLCRERTHKS
Sbjct: 121 ALRSYSLNMSTDFIPMSVFSESSQGKFTIEGKILNKFDMKPHDQNLERYGKLCRERTHKS 180

Query: 181 MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFR 240
           MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIF+
Sbjct: 181 MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFK 240

Query: 241 LFERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYE 300
           LFERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTYE
Sbjct: 241 LFERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTYE 277

Query: 301 LKPEYKESGEETKPR 315
           LKPEY+ES E+TKPR
Sbjct: 301 LKPEYRESSEDTKPR 277

BLAST of Lag0029815 vs. NCBI nr
Match: XP_031741396.1 (general transcription factor IIF subunit 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 471.1 bits (1211), Expect = 7.5e-129
Identity = 260/355 (73.24%), Postives = 276/355 (77.75%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQSNAN-VVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPS 60
           MDVD KS DR +SRK Q+N N +VVG DF+ETNKADRAMWLLKCPQ VTRALSNS D PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNSIVVGTDFLETNKADRAMWLLKCPQLVTRALSNSPDAPS 60

Query: 61  RAVAKVIVSVDPLQSNDDDDSSSTE-----------DRWFSEHGD---QEFMEIGKG--- 120
           R VAKVIVSVDPLQSNDDDDSSSTE           D+    HG    ++  +  KG   
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTEVVSFQDPNLLIDKVAGIHGSFSPEQAEKFFKGPGG 120

Query: 121 -----------------------QSNLMFTMELSGTDSGNALRSYSLNMSTDFIPMSVFS 180
                                  Q++ MFTMEL+ TDSGNALRSYSLNMSTDFIPMSVFS
Sbjct: 121 PDPNPIDIWRVEKSNSRLTVFAFQNSEMFTMELASTDSGNALRSYSLNMSTDFIPMSVFS 180

Query: 181 ESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRP 240
           ES+QGKFT+EGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRP
Sbjct: 181 ESSQGKFTIEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRP 240

Query: 241 MPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQP 300
           MPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIF+LFERQ YWTSKQLIQETDQP
Sbjct: 241 MPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFKLFERQPYWTSKQLIQETDQP 300

Query: 301 EAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEETKPR 315
           E               QYMKEILKDLCVYNNKGVHQGTYELKPEY+ES E+TKPR
Sbjct: 301 E---------------QYMKEILKDLCVYNNKGVHQGTYELKPEYRESSEDTKPR 340

BLAST of Lag0029815 vs. ExPASy Swiss-Prot
Match: Q8R0A0 (General transcription factor IIF subunit 2 OS=Mus musculus OX=10090 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 6.0e-12
Identity = 52/178 (29.21%), Postives = 89/178 (50.00%), Query Frame = 0

Query: 135 MSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTG 194
           ++VF+ES+  K ++EG ++ + + +P     E Y KL R +  +S    R  Q +D V  
Sbjct: 93  LTVFTESSSDKLSLEGIVVQRAECRPAAS--ENYMKLKRLQIEESSKPVRLSQQLDKVVT 152

Query: 195 GHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQ 254
            + +P+       +    ++K   K  + KR R ++  +  ++F  FE+  Y+  K L+ 
Sbjct: 153 TNYKPVANH---QYNIEYERK---KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVD 212

Query: 255 ETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEETK 313
            T QP                 Y+KEILK++ + N KG+H+ T+ELKPEY+    E K
Sbjct: 213 ITKQPVG---------------YLKEILKEIGIQNVKGIHKNTWELKPEYRHYQTEEK 247

BLAST of Lag0029815 vs. ExPASy Swiss-Prot
Match: P13984 (General transcription factor IIF subunit 2 OS=Homo sapiens OX=9606 GN=GTF2F2 PE=1 SV=2)

HSP 1 Score: 72.4 bits (176), Expect = 1.0e-11
Identity = 53/177 (29.94%), Postives = 90/177 (50.85%), Query Frame = 0

Query: 135 MSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTG 194
           ++VF+ES+  K ++EG ++ + + +P     E Y +L R +  +S    R  Q +D V  
Sbjct: 93  LTVFTESSSDKLSLEGIVVQRAECRPAAS--ENYMRLKRLQIEESSKPVRLSQQLDKVVT 152

Query: 195 GHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQ 254
            + +P+       +    ++K   K  + KR R ++  +  ++F  FE+  Y+  K L+ 
Sbjct: 153 TNYKPVANH---QYNIEYERK---KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVD 212

Query: 255 ETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKE-SGEE 311
            T QP                 Y+KEILK++ V N KG+H+ T+ELKPEY+   GEE
Sbjct: 213 ITKQPVV---------------YLKEILKEIGVQNVKGIHKNTWELKPEYRHYQGEE 246

BLAST of Lag0029815 vs. ExPASy Swiss-Prot
Match: Q01750 (General transcription factor IIF subunit 2 OS=Rattus norvegicus OX=10116 GN=Gtf2f2 PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.3e-11
Identity = 52/178 (29.21%), Postives = 88/178 (49.44%), Query Frame = 0

Query: 135 MSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTG 194
           ++VF+ES+  K ++EG ++ + + +P     E Y KL R +  +S    R  Q  D V  
Sbjct: 93  LTVFTESSSDKLSLEGIVVQRAECRPAAS--ENYMKLKRLQIEESSKPVRLSQQADKVVT 152

Query: 195 GHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQ 254
            + +P+       +    ++K   K  + KR R ++  +  ++F  FE+  Y+  K L+ 
Sbjct: 153 TNYKPVANH---QYNIEYERK---KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVD 212

Query: 255 ETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEETK 313
            T QP                 Y+KEILK++ + N KG+H+ T+ELKPEY+    E K
Sbjct: 213 ITKQPVG---------------YLKEILKEIGIQNVKGIHKNTWELKPEYRHYQTEEK 247

BLAST of Lag0029815 vs. ExPASy Swiss-Prot
Match: Q2T9L9 (General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 SV=1)

HSP 1 Score: 71.2 bits (173), Expect = 2.3e-11
Identity = 51/178 (28.65%), Postives = 90/178 (50.56%), Query Frame = 0

Query: 135 MSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTG 194
           ++VF+ES+  K ++EG ++ + + +P     E Y +L R +  +S    R  Q +D V  
Sbjct: 93  LTVFTESSSDKLSLEGIVVQRAECRPAAN--ENYMRLKRLQIEESSKPVRLSQQLDKVVT 152

Query: 195 GHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQ 254
            + +P+       +    ++K   K  + KR R ++  +  ++F  FE+  Y+  K L+ 
Sbjct: 153 TNYKPVANH---QYNIEYERK---KKEDGKRARADKQHVLDMLFSAFEKHQYYNLKDLVD 212

Query: 255 ETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEETK 313
            T QP +               Y+K+ILK++ V N KG+H+ T+ELKPEY+    E K
Sbjct: 213 ITKQPVS---------------YLKDILKEIGVQNVKGIHKNTWELKPEYRHYQVEEK 247

BLAST of Lag0029815 vs. ExPASy Swiss-Prot
Match: P41900 (General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN=TfIIFbeta PE=2 SV=2)

HSP 1 Score: 68.6 bits (166), Expect = 1.5e-10
Identity = 51/168 (30.36%), Postives = 83/168 (49.40%), Query Frame = 0

Query: 145 KFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMRPMPGMD 204
           K  +EG+I+ K + +P   N   Y KL  E   K+    R++Q ID +   + +P+    
Sbjct: 121 KLYMEGRIVQKLECRPIADNC--YMKLKLESIRKASEPQRRVQPIDKIV-QNFKPVKD-H 180

Query: 205 VLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQETDQPEAETS 264
             +    E+KK     +E K+ R ++  +  ++F  FE+  Y+  K L++ T+QP +   
Sbjct: 181 AHNIEYRERKK-----AEGKKARDDKNAVMDMLFHAFEKHQYYNIKDLVKITNQPIS--- 240

Query: 265 RAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEETK 313
                       Y+KEILKD+C YN K  H+  +ELK EY+    E K
Sbjct: 241 ------------YLKEILKDVCDYNMKNPHKNMWELKKEYRHYKTEEK 264

BLAST of Lag0029815 vs. ExPASy TrEMBL
Match: A0A6J1BZ89 (Transcription initiation factor IIF subunit beta OS=Momordica charantia OX=3673 GN=LOC111006053 PE=3 SV=1)

HSP 1 Score: 478.4 bits (1230), Expect = 2.3e-131
Identity = 257/316 (81.33%), Postives = 262/316 (82.91%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQSNANVV--VGADFVETNKADRAMWLLKCPQAVTRALSNSSDGP 60
           MDVDDK  DR SSRKLQ+N N V   G DF+ETNKADRAMWLLKCPQAVTRALSNS DGP
Sbjct: 1   MDVDDKPIDRCSSRKLQTNNNSVSGTGTDFLETNKADRAMWLLKCPQAVTRALSNSPDGP 60

Query: 61  SRAVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSG 120
           SR VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+GTDSG
Sbjct: 61  SRPVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELAGTDSG 120

Query: 121 NALRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHK 180
           N+LRSYSLNMSTDFIPMSVFSES  GKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHK
Sbjct: 121 NSLRSYSLNMSTDFIPMSVFSESPPGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHK 180

Query: 181 SMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIF 240
           SMTKSRQIQVIDHVTGGHMRPMPGMDV SFGAAEKKKVVSKGSETKRLRKERGELEKIIF
Sbjct: 181 SMTKSRQIQVIDHVTGGHMRPMPGMDVTSFGAAEKKKVVSKGSETKRLRKERGELEKIIF 240

Query: 241 RLFERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTY 300
           RLFERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTY
Sbjct: 241 RLFERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTY 278

Query: 301 ELKPEYKESGEETKPR 315
           ELKPEYKESG+ETK R
Sbjct: 301 ELKPEYKESGDETKSR 278

BLAST of Lag0029815 vs. ExPASy TrEMBL
Match: A0A1S3CPB8 (Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC103503304 PE=3 SV=1)

HSP 1 Score: 477.2 bits (1227), Expect = 5.1e-131
Identity = 255/315 (80.95%), Postives = 263/315 (83.49%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQ-SNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPS 60
           MDVD KS DR +SRK Q +N N+VVG DF+ETNKADRAMWLLKCPQ VTRALSNS D PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNNIVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDAPS 60

Query: 61  RAVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGN 120
           R VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+ TDSGN
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELASTDSGN 120

Query: 121 ALRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKS 180
           ALRSY+LNMSTDFIPMSVFSES+QGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKS
Sbjct: 121 ALRSYTLNMSTDFIPMSVFSESSQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKS 180

Query: 181 MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFR 240
           MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIF+
Sbjct: 181 MTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFK 240

Query: 241 LFERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYE 300
           LFERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTYE
Sbjct: 241 LFERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTYE 277

Query: 301 LKPEYKESGEETKPR 315
           LKPEYKES EETKPR
Sbjct: 301 LKPEYKESSEETKPR 277

BLAST of Lag0029815 vs. ExPASy TrEMBL
Match: A0A1S3CPE2 (Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC103503304 PE=3 SV=1)

HSP 1 Score: 470.7 bits (1210), Expect = 4.8e-129
Identity = 255/321 (79.44%), Postives = 263/321 (81.93%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQ-SNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPS 60
           MDVD KS DR +SRK Q +N N+VVG DF+ETNKADRAMWLLKCPQ VTRALSNS D PS
Sbjct: 1   MDVDGKSIDRSTSRKHQTNNNNIVVGTDFLETNKADRAMWLLKCPQVVTRALSNSPDAPS 60

Query: 61  RAVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGN 120
           R VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+ TDSGN
Sbjct: 61  RPVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELASTDSGN 120

Query: 121 ALRSYSLNMSTDFIPMSVFSESTQ------GKFTVEGKILNKFDMKPHDQNLERYGKLCR 180
           ALRSY+LNMSTDFIPMSVFSES+Q      GKFTVEGKILNKFDMKPHDQNLERYGKLCR
Sbjct: 121 ALRSYTLNMSTDFIPMSVFSESSQDSSFVLGKFTVEGKILNKFDMKPHDQNLERYGKLCR 180

Query: 181 ERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGEL 240
           ERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGEL
Sbjct: 181 ERTHKSMTKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGEL 240

Query: 241 EKIIFRLFERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGV 300
           EKIIF+LFERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGV
Sbjct: 241 EKIIFKLFERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGV 283

Query: 301 HQGTYELKPEYKESGEETKPR 315
           HQGTYELKPEYKES EETKPR
Sbjct: 301 HQGTYELKPEYKESSEETKPR 283

BLAST of Lag0029815 vs. ExPASy TrEMBL
Match: A0A6J1JIQ3 (Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=LOC111487292 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.4e-128
Identity = 250/314 (79.62%), Postives = 257/314 (81.85%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQSNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPSR 60
           MD DDK  DR SSRKL SN++ V G DF+ETNKADRAMWLLKCPQ VTRALSNS D PSR
Sbjct: 1   MDGDDKPIDRGSSRKLHSNSSTVAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60

Query: 61  AVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGNA 120
            VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+ TDSG A
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELADTDSGKA 120

Query: 121 LRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSM 180
           LR+YSLNMSTDFIPMSVFSES QGKFTVEGKILNKFDMKPHDQNL+ YGKLCRERTHKSM
Sbjct: 121 LRAYSLNMSTDFIPMSVFSESAQGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSM 180

Query: 181 TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRL 240
           TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSE KRLRKERGELEKIIFRL
Sbjct: 181 TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRL 240

Query: 241 FERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYEL 300
           FERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTYEL
Sbjct: 241 FERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTYEL 276

Query: 301 KPEYKESGEETKPR 315
           KPEYKE+ EETKPR
Sbjct: 301 KPEYKETCEETKPR 276

BLAST of Lag0029815 vs. ExPASy TrEMBL
Match: A0A6J1HK34 (Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 GN=LOC111464830 PE=3 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 4.0e-128
Identity = 249/314 (79.30%), Postives = 256/314 (81.53%), Query Frame = 0

Query: 1   MDVDDKSTDRFSSRKLQSNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSSDGPSR 60
           MD DDK  DR SSRKL SN++   G DF+ETNKADRAMWLLKCPQ VTRALSNS D PSR
Sbjct: 1   MDGDDKPIDRGSSRKLHSNSSTAAGTDFLETNKADRAMWLLKCPQVVTRALSNSPDDPSR 60

Query: 61  AVAKVIVSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGNA 120
            VAKVIVSVDPLQSNDDDDSSSTE                       FTMEL+ TDSG A
Sbjct: 61  PVAKVIVSVDPLQSNDDDDSSSTE-----------------------FTMELADTDSGKA 120

Query: 121 LRSYSLNMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSM 180
           LR+YSLNMSTDFIPMSVFSES QGKFTVEGKILNKFDMKPHDQNL+ YGKLCRERTHKSM
Sbjct: 121 LRAYSLNMSTDFIPMSVFSESAQGKFTVEGKILNKFDMKPHDQNLDSYGKLCRERTHKSM 180

Query: 181 TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRL 240
           TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSE KRLRKERGELEKIIFRL
Sbjct: 181 TKSRQIQVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSENKRLRKERGELEKIIFRL 240

Query: 241 FERQAYWTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYEL 300
           FERQ YWTSKQLIQETDQPE               QYMKEILKDLCVYNNKGVHQGTYEL
Sbjct: 241 FERQPYWTSKQLIQETDQPE---------------QYMKEILKDLCVYNNKGVHQGTYEL 276

Query: 301 KPEYKESGEETKPR 315
           KPEYKE+ EETKPR
Sbjct: 301 KPEYKETCEETKPR 276

BLAST of Lag0029815 vs. TAIR 10
Match: AT3G52270.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 256.5 bits (654), Expect = 2.7e-68
Identity = 145/304 (47.70%), Postives = 192/304 (63.16%), Query Frame = 0

Query: 15  KLQSNANVVVGADFVETNKADRAMWLLKCPQAVTRALSNSS--------DGPSRAVAKVI 74
           K++ N N     + +ET  A+R+M L+K P  V  +L + S        D P R  AKVI
Sbjct: 14  KVRKNEN-----EALETGLAERSMLLMKAPSLVASSLQSHSFPDDPYRPDDPYRPDAKVI 73

Query: 75  VSVDPLQSNDDDDSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGNALRSYSL 134
           + VDPL   D+                              F MEL+  DSGN  R Y+L
Sbjct: 74  LGVDPLAHEDE---------------------------GTQFVMELARADSGNMPRRYTL 133

Query: 135 NMSTDFIPMSVFSESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQI 194
           +MS DFIPM+VF ES+ GK +VEGKI NKFDM+PH++N+E YG+LCRERT+K M K+RQI
Sbjct: 134 DMSKDFIPMNVFCESSDGKMSVEGKIKNKFDMRPHNENIESYGRLCRERTNKYMGKNRQI 193

Query: 195 QVIDHVTGGHMRPMPGMDVLSFGAAEKKKVVSKGSETKRLRKERGELEKIIFRLFERQAY 254
           QVID+  G HMRPMPGM ++   A EKKK+ ++ SE KR R++R E+E+++F LFERQ+ 
Sbjct: 194 QVIDNARGMHMRPMPGM-IIPTAAPEKKKLTNRTSEMKRTRRDRREMEEVMFNLFERQSN 253

Query: 255 WTSKQLIQETDQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKE 311
           WT + LIQETDQPE               Q++K++LKDLC+YNNKG +QGTYELKPEYK+
Sbjct: 254 WTLRLLIQETDQPE---------------QFLKDLLKDLCIYNNKGSNQGTYELKPEYKK 269

BLAST of Lag0029815 vs. TAIR 10
Match: AT1G75510.1 (Transcription initiation factor IIF, beta subunit )

HSP 1 Score: 221.1 bits (562), Expect = 1.3e-57
Identity = 132/294 (44.90%), Postives = 175/294 (59.52%), Query Frame = 0

Query: 29  VETNKADRAMWLLKCPQAVTRALSN----------SSDGPSRAVAKVIVSVDPLQSNDDD 88
           ++  K+DR++WL+KCP  V +A             SSD P   +AK++  VDPL+     
Sbjct: 7   LDIEKSDRSIWLMKCPVVVDKAWHKIAASSSSSFASSDSPP-DMAKIVREVDPLR----- 66

Query: 89  DSSSTEDRWFSEHGDQEFMEIGKGQSNLMFTMELSGTDSGNALRSYSLNMSTDFIPMSVF 148
           D S  E                       F M + G + GN  + Y+LNM TDF+PM  F
Sbjct: 67  DDSPPE-----------------------FKMYMVGAEYGNMPKCYALNMFTDFVPMGGF 126

Query: 149 SESTQGKFTVEGKILNKFDMKPHDQNLERYGKLCRERTHKSMTKSRQIQVIDHVTGGHMR 208
           S+  QG    EGK+ +KFDMKP+ + +E Y +LCRERT K+M K+RQIQVID+  G HMR
Sbjct: 127 SDVNQGCAAAEGKVDHKFDMKPYGETIEEYARLCRERTSKAMVKNRQIQVIDNDRGVHMR 186

Query: 209 PMPGM-DVLSFGAAEKKKVVS-KGSETKRLRKERGELEKIIFRLFERQAYWTSKQLIQET 268
           PMPGM  ++S  + EK+K    K +E KR R++RGELE I+F+LFE Q  WT KQL+QET
Sbjct: 187 PMPGMLGLVSSNSKEKRKPPPVKQTEVKRTRRDRGELEAIMFKLFEGQPNWTLKQLVQET 246

Query: 269 DQPEAETSRAYRKNLSFLQQYMKEILKDLCVYNNKGVHQGTYELKPEYKESGEE 311
           DQP                Q++KEIL +LCVYN +G +QGTYELKPEYK+S E+
Sbjct: 247 DQP---------------AQFLKEILNELCVYNKRGSNQGTYELKPEYKKSAED 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038889594.11.0e-13382.75general transcription factor IIF subunit 2-like [Benincasa hispida] >XP_03888959... [more]
XP_022133483.14.7e-13181.33general transcription factor IIF subunit 2-like [Momordica charantia][more]
XP_008465665.11.1e-13080.95PREDICTED: general transcription factor IIF subunit 2 isoform X2 [Cucumis melo][more]
XP_004143805.14.0e-13080.32general transcription factor IIF subunit 2 isoform X3 [Cucumis sativus] >KGN5163... [more]
XP_031741396.17.5e-12973.24general transcription factor IIF subunit 2 isoform X2 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
Q8R0A06.0e-1229.21General transcription factor IIF subunit 2 OS=Mus musculus OX=10090 GN=Gtf2f2 PE... [more]
P139841.0e-1129.94General transcription factor IIF subunit 2 OS=Homo sapiens OX=9606 GN=GTF2F2 PE=... [more]
Q017501.3e-1129.21General transcription factor IIF subunit 2 OS=Rattus norvegicus OX=10116 GN=Gtf2... [more]
Q2T9L92.3e-1128.65General transcription factor IIF subunit 2 OS=Bos taurus OX=9913 GN=GTF2F2 PE=2 ... [more]
P419001.5e-1030.36General transcription factor IIF subunit 2 OS=Drosophila melanogaster OX=7227 GN... [more]
Match NameE-valueIdentityDescription
A0A6J1BZ892.3e-13181.33Transcription initiation factor IIF subunit beta OS=Momordica charantia OX=3673 ... [more]
A0A1S3CPB85.1e-13180.95Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A1S3CPE24.8e-12979.44Transcription initiation factor IIF subunit beta OS=Cucumis melo OX=3656 GN=LOC1... [more]
A0A6J1JIQ31.4e-12879.62Transcription initiation factor IIF subunit beta OS=Cucurbita maxima OX=3661 GN=... [more]
A0A6J1HK344.0e-12879.30Transcription initiation factor IIF subunit beta OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT3G52270.12.7e-6847.70Transcription initiation factor IIF, beta subunit [more]
AT1G75510.11.3e-5744.90Transcription initiation factor IIF, beta subunit [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR040450TFIIF beta subunit, HTH domainPFAMPF02270TFIIF_betacoord: 227..261
e-value: 3.0E-7
score: 30.5
coord: 275..305
e-value: 2.5E-7
score: 30.7
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 225..306
e-value: 1.2E-27
score: 97.8
NoneNo IPR availablePANTHERPTHR10445:SF2TRANSCRIPTION INITIATION FACTOR IIF, BETA SUBUNITcoord: 21..311
IPR003196Transcription initiation factor IIF, beta subunitPANTHERPTHR10445GENERAL TRANSCRIPTION FACTOR IIF SUBUNIT 2coord: 21..311
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 226..306
IPR011039Transcription Factor IIF, Rap30/Rap74, interactionSUPERFAMILY50916Rap30/74 interaction domainscoord: 29..161

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0029815.1Lag0029815.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006367 transcription initiation from RNA polymerase II promoter
biological_process GO:0006366 transcription by RNA polymerase II
cellular_component GO:0005674 transcription factor TFIIF complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0008168 methyltransferase activity