Lag0029779 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0029779
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionSorting and assembly machinery component 50-like protein
Locationchr8: 42014596 .. 42017480 (+)
RNA-Seq ExpressionLag0029779
SyntenyLag0029779
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGAACTCTGAGGAAGTAGGAAAAAGTTCCATTAGACCGAACTCCGAGGAGGAGCGAGCAGAGAGAGAACCTTCCAATTTGGAAAATGGCGACGGAGAAGATGAGGACGATGATGAAGTTGTAGAGGAGGATGAGGAGGATGAGGAAGAAGAGGATGAAAATGATGAAGAACCGCCGCGGAGGAAGCCTCTTTCGGATGCTTCCAGATTGCTTGCTCAGAGGTCCAAGTTAGAGAATCTGGTCGAGCGAATGAGGAAGGAGAAGGTCAGACTTCGGGTTCACGATATTTTGATAAAGGGAAACAAGAAGACCAAGGATTCTCTAATCGAAGCCGAAGTGGAGGCTATTAAGAATGCATCAACCATGCAAGAACTGCTGGAGGCCGCGGGCGTAGCCAATGCCAAGCTCCAGAGGCTCGAGATCTTCGACTCTGTTAAGATTACACTTGATTCAGGCCCGCCGGAGCTTCCTGGTACTGCAAATGTCGTTGTCGAGGTCGTGGAGACGAGTAACCCTCTCTCTGGCGAATGTGGTGCTTACACAAAACCTGCGGTAAGATTTTGTTCTGTATATGTGTAGATAGATATCTGTGTCTAATGTGTTAAAAGTGGCATGTTGTTTGATAAGATATTGCTTGATAATGAGGAAAGCGAAGTGCTTTTATAGAGTTGTCTACCCCATTAAATGTCAATGGTTTGGCATCTTCTATGTGGCTTGTGCTTCTCGTGGTTCCCTTTTCAGAAATGCAAATTTCAATCTCTAATATAAGACAGAAATGACTAGCTGCAAATTAGATCTAAGTGTAAGCTTCCCAGGCAGAGAACAATGTTGCTCTTATGCTCCATGTATTTTGTCACTTGTATACTATCTGTGCATAAAACCCATTGCAACTGTTCCGGAATATGAAGTTTGTTGGATTGATATATTACCCTCTTTTCTTGCCTTCAGAATGATTGGTTGTTTAATTCATTATTCAGTTTATCGAGTTTGAGAGTGAAGTGTACTATGGAGTGTTTTGCATAGAAGTAGATGGGAAGAAGAATTATACTGTCCGTGTATAAGCAAAGCACACTTGTTTGTCACTAATAATGTGAAATCAAGCTTGTTTCCTTTTTTCCCCACTCTGACATTTTTATCGGACACCTGTCTAAATCAGTTGTACTTCATGGCACAAAACCTCGTAACTATGGATATTTGAACTTTTTTTAATGCTTATCACATATCAATTAGCAACTGAGTTCACAAACTCTGGTTCTTTTTATAATGTGATTCCTCTGTTTAGATTCAGACAATTACCAAGATAAACTTATTGCTTGTTTAATTGGAATTCGTGCATGGACTATTTGGCTTGCTAACTCTAGTTAGTAGTGGAAGTTTGAAGTGGTATGATGCTTTTGTAGGCTCGTTCGTGGACTTTTGAAGGTTCTGTTAAGTATAAAAATTGGCTTGGATATGGGGATTTATGGGATGGCTCTTTGGCATATGGTCCTAACCAAACATCTGAGGTAAGTGCCGGTGTTTATTTCCCTAGGCTGAAGCGATTTGTGACTCCTTTGGTGGCACGGCTGTCCCTACTTTCCCAAGATTGGTTGGAGTTTTCATCTTACAAAGAGCGATCTTTAGGTCTTTCCCTTGGCCTATATTCTACAAAGTATCATGATTTGGTATACAACCTTGGATGGCGCACCATAACTGACCCTTCACAGATGGCATCTAATTCGATAAGGAGACAACTTGGAAATAGTTTACTTTCTTCTCTAAAATATACTTTTAAGGTTGACAAGAGGAATTCAGCAGTGAGGCCGACTAGAGGATATGCTTTTGTTTCTACTAGTCAAATTGGTGGCCTTGCACCCGATCACCGGAGCCTGCGATTTGTGCGCCAGGTATTCTTCATTTCCCTTTGGTTCTATTGTAACAACCAAAGAATTTCCCAAGCTCATGTGTAGTTATCAGTTTGTAGTTGGACATTCATAGTTGTACTGTGCAATATCTGTTTCTTTCAATGTTGAGGAAAATTTTCTGTTCTCATTTTTTAACAGGAATTTGATCTACGTTATGCTATTCCTTTCGGCTTTGATCGTGCTGCCATGAACTTTGGAGTTTCTGCTGGTCTTGTCCTTCCTTGGGGAAATGGATTCATGAACAAGCCATCGTCCCTTCCAGAACGCTTTTTCTTAGGTGGTGATTTCTCTCCAGTATGCACCATGGGAGGCCCAGCAACTGTATGGGGATTTAAGACACGTGGTATGGGGCCAACTGAACCAAGACGAGAAGTGAGGGATGAAAATAAGGGTGAAAACAGTGATTCCCTTGGAAGGGATTTTGTTGGAGGGGATCTTGCAGTCACTGCTTTTGCTGATCTTTCGTTTGACCTCCCAATTCGCTGGTTAAGAGAACATGGAATTCATGGACACATCTTTGCTGGTGCAGGGAACCTTGCAAAGTTGACTGAAAATGAGTTTCGCAGTTTCTCTTTTCAAAAGTTCTTGGAAACTTTTCGTACCTCTGTAGGAGTTGGGGTTGTAGTTCCGACAAGACTCTTCCGCCTAGAGGTCAGTCAAGATCTCTGAATTGTGTGTTTTATTATTTTGGTACAAAATTTATTAGCCTGGTCTGATCTCTATTTTTATGTCCAAAGAAAAGGCGTGGTCCTTATGGATCATTTGTAATGTCATGCTTGCTGTTTCCTCTTTATAGTTCTATTGAACAGACAACAACGACCGGAAAGTTTGTTGTAGGTTGCCTTTCGATTTGATTTGTATGTGCTACTCATTCTAGTTTGGTTCTATGAACAGGGAAACTTCTACTACATATTGAAGCAACAAGAGCATGATCGTGGAAAAACTGGCTTTCGGTTTAGCATTTCTGCTCCATCATAA

mRNA sequence

ATGACGAACTCTGAGGAAGTAGGAAAAAGTTCCATTAGACCGAACTCCGAGGAGGAGCGAGCAGAGAGAGAACCTTCCAATTTGGAAAATGGCGACGGAGAAGATGAGGACGATGATGAAGTTGTAGAGGAGGATGAGGAGGATGAGGAAGAAGAGGATGAAAATGATGAAGAACCGCCGCGGAGGAAGCCTCTTTCGGATGCTTCCAGATTGCTTGCTCAGAGGTCCAAGTTAGAGAATCTGGTCGAGCGAATGAGGAAGGAGAAGGTCAGACTTCGGGTTCACGATATTTTGATAAAGGGAAACAAGAAGACCAAGGATTCTCTAATCGAAGCCGAAGTGGAGGCTATTAAGAATGCATCAACCATGCAAGAACTGCTGGAGGCCGCGGGCGTAGCCAATGCCAAGCTCCAGAGGCTCGAGATCTTCGACTCTGTTAAGATTACACTTGATTCAGGCCCGCCGGAGCTTCCTGGTACTGCAAATGTCGTTGTCGAGGTCGTGGAGACGAGTAACCCTCTCTCTGGCGAATGTGGTGCTTACACAAAACCTGCGGCTCGTTCGTGGACTTTTGAAGGTTCTGTTAAGTATAAAAATTGGCTTGGATATGGGGATTTATGGGATGGCTCTTTGGCATATGGTCCTAACCAAACATCTGAGGTAAGTGCCGGTGTTTATTTCCCTAGGCTGAAGCGATTTGTGACTCCTTTGGTGGCACGGCTGTCCCTACTTTCCCAAGATTGGTTGGAGTTTTCATCTTACAAAGAGCGATCTTTAGGTCTTTCCCTTGGCCTATATTCTACAAAGTATCATGATTTGGTATACAACCTTGGATGGCGCACCATAACTGACCCTTCACAGATGGCATCTAATTCGATAAGGAGACAACTTGGAAATAGTTTACTTTCTTCTCTAAAATATACTTTTAAGGTTGACAAGAGGAATTCAGCAGTGAGGCCGACTAGAGGATATGCTTTTGTTTCTACTAGTCAAATTGGTGGCCTTGCACCCGATCACCGGAGCCTGCGATTTGTGCGCCAGGAATTTGATCTACGTTATGCTATTCCTTTCGGCTTTGATCGTGCTGCCATGAACTTTGGAGTTTCTGCTGGTCTTGTCCTTCCTTGGGGAAATGGATTCATGAACAAGCCATCGTCCCTTCCAGAACGCTTTTTCTTAGGTGGTGATTTCTCTCCAGTATGCACCATGGGAGGCCCAGCAACTGTATGGGGATTTAAGACACGTGGTATGGGGCCAACTGAACCAAGACGAGAAGTGAGGGATGAAAATAAGGGTGAAAACAGTGATTCCCTTGGAAGGGATTTTGTTGGAGGGGATCTTGCAGTCACTGCTTTTGCTGATCTTTCGTTTGACCTCCCAATTCGCTGGTTAAGAGAACATGGAATTCATGGACACATCTTTGCTGGTGCAGGGAACCTTGCAAAGTTGACTGAAAATGAGTTTCGCAGTTTCTCTTTTCAAAAGTTCTTGGAAACTTTTCGTACCTCTGTAGGAGTTGGGGTTGTAGTTCCGACAAGACTCTTCCGCCTAGAGGGAAACTTCTACTACATATTGAAGCAACAAGAGCATGATCGTGGAAAAACTGGCTTTCGGTTTAGCATTTCTGCTCCATCATAA

Coding sequence (CDS)

ATGACGAACTCTGAGGAAGTAGGAAAAAGTTCCATTAGACCGAACTCCGAGGAGGAGCGAGCAGAGAGAGAACCTTCCAATTTGGAAAATGGCGACGGAGAAGATGAGGACGATGATGAAGTTGTAGAGGAGGATGAGGAGGATGAGGAAGAAGAGGATGAAAATGATGAAGAACCGCCGCGGAGGAAGCCTCTTTCGGATGCTTCCAGATTGCTTGCTCAGAGGTCCAAGTTAGAGAATCTGGTCGAGCGAATGAGGAAGGAGAAGGTCAGACTTCGGGTTCACGATATTTTGATAAAGGGAAACAAGAAGACCAAGGATTCTCTAATCGAAGCCGAAGTGGAGGCTATTAAGAATGCATCAACCATGCAAGAACTGCTGGAGGCCGCGGGCGTAGCCAATGCCAAGCTCCAGAGGCTCGAGATCTTCGACTCTGTTAAGATTACACTTGATTCAGGCCCGCCGGAGCTTCCTGGTACTGCAAATGTCGTTGTCGAGGTCGTGGAGACGAGTAACCCTCTCTCTGGCGAATGTGGTGCTTACACAAAACCTGCGGCTCGTTCGTGGACTTTTGAAGGTTCTGTTAAGTATAAAAATTGGCTTGGATATGGGGATTTATGGGATGGCTCTTTGGCATATGGTCCTAACCAAACATCTGAGGTAAGTGCCGGTGTTTATTTCCCTAGGCTGAAGCGATTTGTGACTCCTTTGGTGGCACGGCTGTCCCTACTTTCCCAAGATTGGTTGGAGTTTTCATCTTACAAAGAGCGATCTTTAGGTCTTTCCCTTGGCCTATATTCTACAAAGTATCATGATTTGGTATACAACCTTGGATGGCGCACCATAACTGACCCTTCACAGATGGCATCTAATTCGATAAGGAGACAACTTGGAAATAGTTTACTTTCTTCTCTAAAATATACTTTTAAGGTTGACAAGAGGAATTCAGCAGTGAGGCCGACTAGAGGATATGCTTTTGTTTCTACTAGTCAAATTGGTGGCCTTGCACCCGATCACCGGAGCCTGCGATTTGTGCGCCAGGAATTTGATCTACGTTATGCTATTCCTTTCGGCTTTGATCGTGCTGCCATGAACTTTGGAGTTTCTGCTGGTCTTGTCCTTCCTTGGGGAAATGGATTCATGAACAAGCCATCGTCCCTTCCAGAACGCTTTTTCTTAGGTGGTGATTTCTCTCCAGTATGCACCATGGGAGGCCCAGCAACTGTATGGGGATTTAAGACACGTGGTATGGGGCCAACTGAACCAAGACGAGAAGTGAGGGATGAAAATAAGGGTGAAAACAGTGATTCCCTTGGAAGGGATTTTGTTGGAGGGGATCTTGCAGTCACTGCTTTTGCTGATCTTTCGTTTGACCTCCCAATTCGCTGGTTAAGAGAACATGGAATTCATGGACACATCTTTGCTGGTGCAGGGAACCTTGCAAAGTTGACTGAAAATGAGTTTCGCAGTTTCTCTTTTCAAAAGTTCTTGGAAACTTTTCGTACCTCTGTAGGAGTTGGGGTTGTAGTTCCGACAAGACTCTTCCGCCTAGAGGGAAACTTCTACTACATATTGAAGCAACAAGAGCATGATCGTGGAAAAACTGGCTTTCGGTTTAGCATTTCTGCTCCATCATAA

Protein sequence

MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPPRRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNASTMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVARLSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFDRAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPTEPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNLAKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFSISAPS
Homology
BLAST of Lag0029779 vs. NCBI nr
Match: XP_038885406.1 (sorting and assembly machinery component 50 homolog [Benincasa hispida] >XP_038885407.1 sorting and assembly machinery component 50 homolog [Benincasa hispida])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.9e-292
Identity = 515/545 (94.50%), Postives = 530/545 (97.25%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPP 60
           M NSEEVG SSI  NSEEE+AEREPSN ENGDGEDED DE VE++EEDEE+ED+NDEE P
Sbjct: 1   MANSEEVGTSSIGLNSEEEQAEREPSNFENGDGEDEDGDEDVEDEEEDEEDEDDNDEELP 60

Query: 61  RRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNA 120
           RRKP+SDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAIKNA
Sbjct: 61  RRKPISDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNTKTKDSLIEAEVEAIKNA 120

Query: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGA 180
           STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGECGA
Sbjct: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGECGA 180

Query: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVAR 240
           YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKR VTPLVAR
Sbjct: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRLVTPLVAR 240

Query: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNS 300
           LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDL YNLGWRT+TDPSQMASNS+RRQLGNS
Sbjct: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTLTDPSQMASNSVRRQLGNS 300

Query: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360
           LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD
Sbjct: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360

Query: 361 RAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPT 420
           RAAMNFGVSAG+VLPWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGMGPT
Sbjct: 361 RAAMNFGVSAGVVLPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGMGPT 420

Query: 421 EPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480
           EPRREVRDENKGEN DSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL
Sbjct: 421 EPRREVRDENKGENDDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480

Query: 481 AKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 540
           AKLTENEFRSFSFQKF+ETFRTSVG G+VVPTRLFRLEGNFYYILKQQ+HDRGKTGFRFS
Sbjct: 481 AKLTENEFRSFSFQKFMETFRTSVGAGIVVPTRLFRLEGNFYYILKQQQHDRGKTGFRFS 540

Query: 541 ISAPS 546
           ISAPS
Sbjct: 541 ISAPS 545

BLAST of Lag0029779 vs. NCBI nr
Match: KAA0036493.1 (sorting and assembly machinery component 50-like protein [Cucumis melo var. makuwa] >TYJ99993.1 sorting and assembly machinery component 50-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 992.3 bits (2564), Expect = 1.7e-285
Identity = 506/545 (92.84%), Postives = 521/545 (95.60%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPP 60
           M NSEEVGKSSI     EE  ER+PSN ENGDGEDED    VE++EEDEE ED NDEEPP
Sbjct: 1   MANSEEVGKSSI-----EEEVERDPSNFENGDGEDED----VEDEEEDEEYEDGNDEEPP 60

Query: 61  RRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNA 120
           + KP++DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAIKNA
Sbjct: 61  QEKPVTDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNTKTKDSLIEAEVEAIKNA 120

Query: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGA 180
           STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGECGA
Sbjct: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGECGA 180

Query: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVAR 240
           YTKPAARSWTFEGS+KYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKR VTPLVAR
Sbjct: 181 YTKPAARSWTFEGSLKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRLVTPLVAR 240

Query: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNS 300
           LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNS+RRQLGNS
Sbjct: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSVRRQLGNS 300

Query: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360
           LLSSLKYTFKVDKRNSAVRPT GYAFVSTSQ+GGLAPDHRSLRFVRQEFDLRYAIPFGFD
Sbjct: 301 LLSSLKYTFKVDKRNSAVRPTSGYAFVSTSQVGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360

Query: 361 RAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPT 420
           RAAMNFGVSAG+V PWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGMGPT
Sbjct: 361 RAAMNFGVSAGVVFPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGMGPT 420

Query: 421 EPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480
           EPRREVRDENK EN+DSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL
Sbjct: 421 EPRREVRDENKDENNDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480

Query: 481 AKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 540
           AKLTENEFRSFSFQKF+ETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS
Sbjct: 481 AKLTENEFRSFSFQKFMETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 536

Query: 541 ISAPS 546
           ISAPS
Sbjct: 541 ISAPS 536

BLAST of Lag0029779 vs. NCBI nr
Match: XP_008456518.1 (PREDICTED: LOW QUALITY PROTEIN: sorting and assembly machinery component 50 homolog [Cucumis melo])

HSP 1 Score: 990.3 bits (2559), Expect = 6.4e-285
Identity = 505/545 (92.66%), Postives = 520/545 (95.41%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPP 60
           M NSEEVGKSSI     EE  ER+PSN ENGDGEDED    VE++EEDEE ED NDEEPP
Sbjct: 1   MANSEEVGKSSI-----EEEVERDPSNFENGDGEDED----VEDEEEDEEYEDGNDEEPP 60

Query: 61  RRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNA 120
           + KP++DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAIKNA
Sbjct: 61  QEKPVTDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNTKTKDSLIEAEVEAIKNA 120

Query: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGA 180
           STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGECGA
Sbjct: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGECGA 180

Query: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVAR 240
           YTKPAARSWTFEGS+KYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKR VTPLVAR
Sbjct: 181 YTKPAARSWTFEGSLKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRLVTPLVAR 240

Query: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNS 300
           LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNS+RRQLGNS
Sbjct: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSVRRQLGNS 300

Query: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360
           LLSSLKYTFKVDKRNSAVRPT GYAFVSTSQ+GGLAPDHRSLRFVRQEFDLRYAIPFGFD
Sbjct: 301 LLSSLKYTFKVDKRNSAVRPTSGYAFVSTSQVGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360

Query: 361 RAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPT 420
           RAAMNFGVSAG+  PWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGMGPT
Sbjct: 361 RAAMNFGVSAGVXFPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGMGPT 420

Query: 421 EPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480
           EPRREVRDENK EN+DSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL
Sbjct: 421 EPRREVRDENKDENNDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480

Query: 481 AKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 540
           AKLTENEFRSFSFQKF+ETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS
Sbjct: 481 AKLTENEFRSFSFQKFMETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 536

Query: 541 ISAPS 546
           ISAPS
Sbjct: 541 ISAPS 536

BLAST of Lag0029779 vs. NCBI nr
Match: XP_004140074.1 (sorting and assembly machinery component 50 homolog [Cucumis sativus] >KGN47843.1 hypothetical protein Csa_002751 [Cucumis sativus])

HSP 1 Score: 990.3 bits (2559), Expect = 6.4e-285
Identity = 507/545 (93.03%), Postives = 520/545 (95.41%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPP 60
           M NSEEVGKSSI     EE  EREPSN ENGDGEDED    VE+DEEDEE ED NDEE P
Sbjct: 1   MANSEEVGKSSI-----EEEVEREPSNFENGDGEDED----VEDDEEDEEYEDGNDEELP 60

Query: 61  RRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNA 120
           + KP++DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAIK A
Sbjct: 61  QGKPVTDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNTKTKDSLIEAEVEAIKTA 120

Query: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGA 180
           STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGECGA
Sbjct: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGECGA 180

Query: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVAR 240
           YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKR VTPLVAR
Sbjct: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRLVTPLVAR 240

Query: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNS 300
           LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNS+RRQLGNS
Sbjct: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSVRRQLGNS 300

Query: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360
           LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQ+GGLAPDHRSLRFVRQEFDLRYAIPFGFD
Sbjct: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQVGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360

Query: 361 RAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPT 420
           RAAMNFGVSAG+V PWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGMGPT
Sbjct: 361 RAAMNFGVSAGVVFPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGMGPT 420

Query: 421 EPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480
           EPRREVRDENK +N+DSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL
Sbjct: 421 EPRREVRDENKDDNNDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480

Query: 481 AKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 540
           AKLTENEFRSFSFQKF+ETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS
Sbjct: 481 AKLTENEFRSFSFQKFMETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 536

Query: 541 ISAPS 546
           ISAPS
Sbjct: 541 ISAPS 536

BLAST of Lag0029779 vs. NCBI nr
Match: KAG6578810.1 (Sorting and assembly machinery component 50-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 989.9 bits (2558), Expect = 8.4e-285
Identity = 505/548 (92.15%), Postives = 529/548 (96.53%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNS--EEERAEREPSNLENGDGEDED-DDEVVEEDEEDEEEEDENDE 60
           M NSEEVGK++IR +S  EEE  EREPSN ENGDGEDE+ +DE  EEDEEDE++ED+++E
Sbjct: 1   MENSEEVGKNTIRTSSEEEEEEVEREPSNFENGDGEDEEVEDE--EEDEEDEDDEDDDEE 60

Query: 61  EPPRRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAI 120
           EPPRRKP+ DASRL+AQRSKLENLVERMRKEKVRLRVHD+LIKGN KTKDSLIEAEVEAI
Sbjct: 61  EPPRRKPILDASRLIAQRSKLENLVERMRKEKVRLRVHDVLIKGNSKTKDSLIEAEVEAI 120

Query: 121 KNASTMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGE 180
           KNASTMQELLEAAGVANA LQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGE
Sbjct: 121 KNASTMQELLEAAGVANANLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGE 180

Query: 181 CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPL 240
           CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVY PRLKR +TPL
Sbjct: 181 CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYLPRLKRLMTPL 240

Query: 241 VARLSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQL 300
           VARLSLLSQDW+EFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNSIRRQL
Sbjct: 241 VARLSLLSQDWMEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSIRRQL 300

Query: 301 GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF 360
           GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF
Sbjct: 301 GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF 360

Query: 361 GFDRAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGM 420
           GFDRAAMNFGVSAG+VLPWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGM
Sbjct: 361 GFDRAAMNFGVSAGVVLPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGM 420

Query: 421 GPTEPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGA 480
           GPTEPRREVRDENKGE++D+LGRDFVGGDLAVTAFADLSFDLP+RWLREHGIHGHIFAGA
Sbjct: 421 GPTEPRREVRDENKGEDNDALGRDFVGGDLAVTAFADLSFDLPLRWLREHGIHGHIFAGA 480

Query: 481 GNLAKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGF 540
           GNLAKLTENEFRSFSFQKF ETFRTSVGVG+VVPTRLFRLEGNFYYILKQQEHDRGKTGF
Sbjct: 481 GNLAKLTENEFRSFSFQKFRETFRTSVGVGIVVPTRLFRLEGNFYYILKQQEHDRGKTGF 540

Query: 541 RFSISAPS 546
           RFSISAPS
Sbjct: 541 RFSISAPS 546

BLAST of Lag0029779 vs. ExPASy Swiss-Prot
Match: Q8BGH2 (Sorting and assembly machinery component 50 homolog OS=Mus musculus OX=10090 GN=Samm50 PE=1 SV=1)

HSP 1 Score: 131.3 bits (329), Expect = 3.2e-29
Identity = 132/464 (28.45%), Postives = 205/464 (44.18%), Query Frame = 0

Query: 94  VHDILIKGNKKTKDSLIEAEVEAIKNASTMQELLEAAGVANAKLQRLEIFDSVKITLDS- 153
           V  +   G  +TKD +I  E+  +  A  + E++  +  A  KL RL IF  V + +D+ 
Sbjct: 47  VQHVHFDGLGRTKDDIIICEIGEVFKAKNLIEVMRRSHEAREKLLRLGIFRQVDVLIDTC 106

Query: 154 -GPPELPGTANVVVEVVETSNPLSGECGAYTKPAARSWTFEGS----VKYKNWLGYGDLW 213
            G   LP   +V  EV E    L    G+Y      +   EGS    +K  N LG  +  
Sbjct: 107 HGEDALPNGLDVTFEVTE----LRRLTGSYNTMVGNN---EGSMVLGLKLPNLLGRAEKV 166

Query: 214 DGSLAYGPNQTSEVSAGVYF--PRLKRFVTPLVARLSLLSQDWLEFSSYKERSLGL---- 273
               +YG   T E S G+ F  P+   F       L  ++  +  +SS +E   G+    
Sbjct: 167 TFQFSYG---TKETSYGLSFFKPQPGNFERNFSVNLYKVTGQF-PWSSLRETDRGVSAEY 226

Query: 274 SLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNSLLSSLKYTFKVDKRNSAVRPT 333
           S  L+ T  H + +   WR +   S+ AS ++R++ G+SL SSL +   +D RNS++ P 
Sbjct: 227 SFPLWKTS-HTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPR 286

Query: 334 RGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFDRAAMNFGVSAGLVLPWGNGFM 393
           RG  F    ++ G       + F++++F+L+   P   D +  +  +  G+++P G    
Sbjct: 287 RGALFKVNQELAGYTGG--DVSFIKEDFELQLNKPLALD-SVFSTSLWGGMLVPIG---- 346

Query: 394 NKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPTEPRREVRDENKGENSDSLGRD 453
           +KPSS+ +RF+L          GGP +V GF    +GP         +++G        D
Sbjct: 347 DKPSSIADRFYL----------GGPTSVRGFSMHSIGP---------QSEG--------D 406

Query: 454 FVGGDLAVTAFADLSFDLPIRWLREHGIHG-----HIFAGAGNLAKLTENEFRSFSFQKF 513
           ++GG+        L   LP R     G  G     H F  AGNL  L   E      +K 
Sbjct: 407 YLGGEAYWAGGLHLYTPLPFR--PGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKL 462

Query: 514 LETFRTSVGVGVVVPT-RLFRLEGNFYYILKQQEHDRGKTGFRF 540
            E  R S G GVV+    + RLE N+   +  Q  DR   G +F
Sbjct: 467 AECIRWSYGAGVVLRLGNIARLELNYCIPMGVQGGDRICDGVQF 462

BLAST of Lag0029779 vs. ExPASy Swiss-Prot
Match: Q6PA35 (Sorting and assembly machinery component 50 homolog B OS=Xenopus laevis OX=8355 GN=samm50-b PE=2 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.7e-28
Identity = 134/491 (27.29%), Postives = 223/491 (45.42%), Query Frame = 0

Query: 67  DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNASTMQEL 126
           DA  +  +  K + ++E   K+ V  RVH    +G  +TKD LI  E+  +  A  + E+
Sbjct: 24  DADLVEVEPEKKQEILE--NKDVVVQRVH---FEGLGRTKDDLIAHEIGQVFKAKNLIEV 83

Query: 127 LEAAGVANAKLQRLEIFDSVKITLDS--GPPELPGTANVVVEVVETSNPLSGECGAYTKP 186
           +  +  A  KL RL +F +V++ +D+  G   +P   +V  EV E    L    G+Y   
Sbjct: 84  MRKSHEAREKLLRLGVFRNVEVLIDTSEGEDAVPNGLDVTFEVTE----LRRLTGSYNTM 143

Query: 187 AARSWTFEGS----VKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYF--PRLKRFVTPLV 246
              +   EGS    +K+ N  G  +      +YG   T E S G+ F  P++  F     
Sbjct: 144 VGNN---EGSMVLGLKFPNLFGRAEKMTFQFSYG---TKETSYGLSFFKPQVGNFERNFS 203

Query: 247 ARLSLLSQDWLEFSSYKERSLGLS----LGLYSTKYHDLVYNLGWRTITDPSQMASNSIR 306
             L  ++  +  +SS +E   G+S      ++ T  H L +   WR +   ++ AS +IR
Sbjct: 204 VNLYKVTGQF-PWSSLRETDRGVSAEINFPIWKTS-HTLKWEGVWRELGCLARTASFAIR 263

Query: 307 RQLGNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYA 366
            + G++L SSL +T  +D RN+++ P RG       ++ G       + F++++F+L+  
Sbjct: 264 EESGHTLKSSLSHTMVIDSRNASILPKRGALLKINQELAGYTGG--DVSFLKEDFELQLN 323

Query: 367 IPFGFDRAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKT 426
               +D + ++  +  G+++P G    ++PSS+ +RF+L          GGP +V GF  
Sbjct: 324 KQLAWD-SVLSTSLWGGMLVPVG----DRPSSIADRFYL----------GGPTSVRGFSM 383

Query: 427 RGMGPTEPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHG--- 486
             +GP         +++G        D++GG+        L   LP R  R  G  G   
Sbjct: 384 YSIGP---------QSEG--------DYLGGEAYWAGGVHLYTPLPFRPGR--GGFGDLF 443

Query: 487 --HIFAGAGNLAKLTENEFRSFSFQKFLETFRTSVGVGVVVPT-RLFRLEGNFYYILKQQ 540
             H F  AGNL  L   E      Q+  E  R S G G+V+    + RLE N+   +  Q
Sbjct: 444 RTHFFLNAGNLCNLNYGEGPRAHLQRLAECIRWSYGAGLVLRLGNIARLELNYCIPMGVQ 461

BLAST of Lag0029779 vs. ExPASy Swiss-Prot
Match: Q6P806 (Sorting and assembly machinery component 50 homolog OS=Xenopus tropicalis OX=8364 GN=samm50 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 6.1e-28
Identity = 135/491 (27.49%), Postives = 222/491 (45.21%), Query Frame = 0

Query: 67  DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNASTMQEL 126
           DA  +  +  K + ++E   K+ V  RVH    +G  +TKD LI  E+  +  A  + E+
Sbjct: 24  DADLVEVEPQKKQEILE--NKDVVVQRVH---FEGLGRTKDDLIAHEIGQVFKAKNLIEV 83

Query: 127 LEAAGVANAKLQRLEIFDSVKITLDS--GPPELPGTANVVVEVVETSNPLSGECGAYTKP 186
           +  +  A  KL RL +F +V++ +D+  G   LP   +V  EV E    L    G+Y   
Sbjct: 84  MRKSHEAREKLLRLGVFRNVEVLIDTCEGEDALPNGLDVTFEVTE----LRRLTGSYNTM 143

Query: 187 AARSWTFEGS----VKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYF--PRLKRFVTPLV 246
              +   EGS    +K  N  G  +      +YG   T E S G+ F  P++  F     
Sbjct: 144 VGNN---EGSMVLGLKLPNLFGRAEKMTFQFSYG---TKETSYGLSFFKPQVGNFERNFS 203

Query: 247 ARLSLLSQDWLEFSSYKERSLGLS----LGLYSTKYHDLVYNLGWRTITDPSQMASNSIR 306
             L  ++  +  +SS +E   G+S      ++ T  H L +   WR +   ++ AS +IR
Sbjct: 204 VNLYKVTGQF-PWSSLRETDRGVSAEINFPIWKTS-HTLKWEGVWRELGCLARTASFAIR 263

Query: 307 RQLGNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYA 366
            + G++L SSL +T  +D RN+++ P RG       ++ G       + F++++F+L+  
Sbjct: 264 EESGHTLKSSLSHTMVIDSRNTSILPKRGALLKINQELAGYTGG--DVSFLKEDFELQLN 323

Query: 367 IPFGFDRAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKT 426
               +D + ++  +  G+++P G    +KPSS+ +RF+L          GGP +V GF  
Sbjct: 324 KQLAWD-SVLSTSLWGGMLVPIG----DKPSSIADRFYL----------GGPTSVRGFSM 383

Query: 427 RGMGPTEPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHG--- 486
             +GP         +++G        D++GG+        L   LP R  R  G  G   
Sbjct: 384 YSIGP---------QSEG--------DYLGGEAYWAGGMHLYTPLPFRPGR--GGFGDLF 443

Query: 487 --HIFAGAGNLAKLTENEFRSFSFQKFLETFRTSVGVGVVVPT-RLFRLEGNFYYILKQQ 540
             H F  AGNL  L   E      ++  E  R S G G+V+    + RLE N+   +  Q
Sbjct: 444 RTHFFLNAGNLCNLNYGEGPRAHLRRLAECIRWSYGAGIVLRLGNIARLELNYCIPMGVQ 461

BLAST of Lag0029779 vs. ExPASy Swiss-Prot
Match: Q9Y512 (Sorting and assembly machinery component 50 homolog OS=Homo sapiens OX=9606 GN=SAMM50 PE=1 SV=3)

HSP 1 Score: 126.3 bits (316), Expect = 1.0e-27
Identity = 129/464 (27.80%), Postives = 203/464 (43.75%), Query Frame = 0

Query: 94  VHDILIKGNKKTKDSLIEAEVEAIKNASTMQELLEAAGVANAKLQRLEIFDSVKITLDS- 153
           V  +   G  +TKD +I  E+  +  A  + E++  +  A  KL RL IF  V + +D+ 
Sbjct: 47  VQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLIDTC 106

Query: 154 -GPPELPGTANVVVEVVETSNPLSGECGAYTKPAARSWTFEGS----VKYKNWLGYGDLW 213
            G   LP   +V  EV E    L    G+Y      +   EGS    +K  N LG  +  
Sbjct: 107 QGDDALPNGLDVTFEVTE----LRRLTGSYNTMVGNN---EGSMVLGLKLPNLLGRAEKV 166

Query: 214 DGSLAYGPNQTSEVSAGVYF--PRLKRFVTPLVARLSLLSQDWLEFSSYKERSLGL---- 273
               +YG   T E S G+ F  PR   F       L  ++  +  +SS +E   G+    
Sbjct: 167 TFQFSYG---TKETSYGLSFFKPRPGNFERNFSVNLYKVTGQF-PWSSLRETDRGMSAEY 226

Query: 274 SLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNSLLSSLKYTFKVDKRNSAVRPT 333
           S  ++ T  H + +   WR +   S+ AS ++R++ G+SL SSL +   +D RNS++ P 
Sbjct: 227 SFPIWKTS-HTVKWEGVWRELGCLSRTASFAVRKESGHSLKSSLSHAMVIDSRNSSILPR 286

Query: 334 RGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFDRAAMNFGVSAGLVLPWGNGFM 393
           RG       ++ G       + F++++F+L+      FD +  +     G+++P G    
Sbjct: 287 RGALLKVNQELAGYTGG--DVSFIKEDFELQLNKQLIFD-SVFSASFWGGMLVPIG---- 346

Query: 394 NKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPTEPRREVRDENKGENSDSLGRD 453
           +KPSS+ +RF+L          GGP ++ GF    +GP         +++G        D
Sbjct: 347 DKPSSIADRFYL----------GGPTSIRGFSMHSIGP---------QSEG--------D 406

Query: 454 FVGGDLAVTAFADLSFDLPIRWLREHGIHG-----HIFAGAGNLAKLTENEFRSFSFQKF 513
           ++GG+        L   LP R     G  G     H F  AGNL  L   E      +K 
Sbjct: 407 YLGGEAYWAGGLHLYTPLPFR--PGQGGFGELFRTHFFLNAGNLCNLNYGEGPKAHIRKL 462

Query: 514 LETFRTSVGVGVVVPT-RLFRLEGNFYYILKQQEHDRGKTGFRF 540
            E  R S G G+V+    + RLE N+   +  Q  DR   G +F
Sbjct: 467 AECIRWSYGAGIVLRLGNIARLELNYCVPMGVQTGDRICDGVQF 462

BLAST of Lag0029779 vs. ExPASy Swiss-Prot
Match: Q5U3I0 (Sorting and assembly machinery component 50 homolog B OS=Danio rerio OX=7955 GN=samm50b PE=2 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 5.2e-27
Identity = 126/463 (27.21%), Postives = 206/463 (44.49%), Query Frame = 0

Query: 94  VHDILIKGNKKTKDSLIEAEVEAIKNASTMQELLEAAGVANAKLQRLEIFDSVKITLD-- 153
           V  + I+G  +TK+  +  E+  +  A  + E++  +  A  +L RL IF  V++ +D  
Sbjct: 47  VQHVNIEGLGRTKEDYLGYEISDVFTARNLVEVMRKSHEARQRLLRLGIFRDVEVVIDIS 106

Query: 154 SGPPELPGTANVVVEVVETSNPLSGECGAYTKPAARSWTFEGS----VKYKNWLGYGDLW 213
            G   LP   +V  EV E    L    G+Y      +   EGS    +K  N LG G+  
Sbjct: 107 EGADALPNGLDVTFEVTE----LKRLTGSYNTMVGNN---EGSMVLGIKLPNMLGRGEKL 166

Query: 214 DGSLAYGPNQTSEVSAGVYF--PRLKRFVTPLVARLSLLSQDWLEFSSYKERSLG----L 273
               +YG   T E S G+ F  P+   F       L  ++  +  +SS KE   G    L
Sbjct: 167 TFQFSYG---TKETSYGLSFFKPQSGYFERNFTVNLYKVTGQF-PWSSLKETDRGVSTEL 226

Query: 274 SLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNSLLSSLKYTFKVDKRNSAVRPT 333
           SL L+ T  H L +   WR +   ++ AS ++R + G++L S+L +T  VD RNSA+ P 
Sbjct: 227 SLPLWMTD-HTLKWEGVWRELGCLARSASFAVREESGHTLKSALSHTMVVDTRNSAILPR 286

Query: 334 RGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFDRAAMNFGVSAGLVLPWGNGFM 393
           +G       ++ G         F++++ +L+    F +  + ++  +  G++ P G    
Sbjct: 287 KGALLRINQELAGYTGG--DANFLKEDVELQLNKQFIWG-SVLSASLWGGMIYPLG---- 346

Query: 394 NKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPTEPRREVRDENKGENSDSLGRD 453
            +P+S+ +RF+L          GGP +V GF    +GP         +++G        D
Sbjct: 347 AQPTSIADRFYL----------GGPTSVRGFGMYSIGP---------QSEG--------D 406

Query: 454 FVGGDLAVTAFADLSFDLPIRWLREHGI----HGHIFAGAGNLAKLTENEFRSFSFQKFL 513
           ++GG+        L   LP R   + G+      H F  AGN+  L   E      QK  
Sbjct: 407 YLGGEAYWAGGLHLYTPLPFR-SNKGGLADLFRTHFFLNAGNICNLNYGEGPRAHLQKLA 462

Query: 514 ETFRTSVGVGVVVPT-RLFRLEGNFYYILKQQEHDRGKTGFRF 540
           E  R S G G+++    + RLE N+ + +  Q  DR   G +F
Sbjct: 467 ECIRWSYGAGIILRLGNIARLELNYCFPMGVQSGDRICDGVQF 462

BLAST of Lag0029779 vs. ExPASy TrEMBL
Match: A0A5A7T0T1 (Sorting and assembly machinery component 50-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold360G00380 PE=4 SV=1)

HSP 1 Score: 992.3 bits (2564), Expect = 8.2e-286
Identity = 506/545 (92.84%), Postives = 521/545 (95.60%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPP 60
           M NSEEVGKSSI     EE  ER+PSN ENGDGEDED    VE++EEDEE ED NDEEPP
Sbjct: 1   MANSEEVGKSSI-----EEEVERDPSNFENGDGEDED----VEDEEEDEEYEDGNDEEPP 60

Query: 61  RRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNA 120
           + KP++DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAIKNA
Sbjct: 61  QEKPVTDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNTKTKDSLIEAEVEAIKNA 120

Query: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGA 180
           STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGECGA
Sbjct: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGECGA 180

Query: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVAR 240
           YTKPAARSWTFEGS+KYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKR VTPLVAR
Sbjct: 181 YTKPAARSWTFEGSLKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRLVTPLVAR 240

Query: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNS 300
           LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNS+RRQLGNS
Sbjct: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSVRRQLGNS 300

Query: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360
           LLSSLKYTFKVDKRNSAVRPT GYAFVSTSQ+GGLAPDHRSLRFVRQEFDLRYAIPFGFD
Sbjct: 301 LLSSLKYTFKVDKRNSAVRPTSGYAFVSTSQVGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360

Query: 361 RAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPT 420
           RAAMNFGVSAG+V PWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGMGPT
Sbjct: 361 RAAMNFGVSAGVVFPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGMGPT 420

Query: 421 EPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480
           EPRREVRDENK EN+DSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL
Sbjct: 421 EPRREVRDENKDENNDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480

Query: 481 AKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 540
           AKLTENEFRSFSFQKF+ETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS
Sbjct: 481 AKLTENEFRSFSFQKFMETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 536

Query: 541 ISAPS 546
           ISAPS
Sbjct: 541 ISAPS 536

BLAST of Lag0029779 vs. ExPASy TrEMBL
Match: A0A0A0KE64 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G406550 PE=4 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.1e-285
Identity = 507/545 (93.03%), Postives = 520/545 (95.41%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPP 60
           M NSEEVGKSSI     EE  EREPSN ENGDGEDED    VE+DEEDEE ED NDEE P
Sbjct: 1   MANSEEVGKSSI-----EEEVEREPSNFENGDGEDED----VEDDEEDEEYEDGNDEELP 60

Query: 61  RRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNA 120
           + KP++DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAIK A
Sbjct: 61  QGKPVTDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNTKTKDSLIEAEVEAIKTA 120

Query: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGA 180
           STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGECGA
Sbjct: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGECGA 180

Query: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVAR 240
           YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKR VTPLVAR
Sbjct: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRLVTPLVAR 240

Query: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNS 300
           LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNS+RRQLGNS
Sbjct: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSVRRQLGNS 300

Query: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360
           LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQ+GGLAPDHRSLRFVRQEFDLRYAIPFGFD
Sbjct: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQVGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360

Query: 361 RAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPT 420
           RAAMNFGVSAG+V PWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGMGPT
Sbjct: 361 RAAMNFGVSAGVVFPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGMGPT 420

Query: 421 EPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480
           EPRREVRDENK +N+DSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL
Sbjct: 421 EPRREVRDENKDDNNDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480

Query: 481 AKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 540
           AKLTENEFRSFSFQKF+ETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS
Sbjct: 481 AKLTENEFRSFSFQKFMETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 536

Query: 541 ISAPS 546
           ISAPS
Sbjct: 541 ISAPS 536

BLAST of Lag0029779 vs. ExPASy TrEMBL
Match: A0A1S3C310 (LOW QUALITY PROTEIN: sorting and assembly machinery component 50 homolog OS=Cucumis melo OX=3656 GN=LOC103496447 PE=4 SV=1)

HSP 1 Score: 990.3 bits (2559), Expect = 3.1e-285
Identity = 505/545 (92.66%), Postives = 520/545 (95.41%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPP 60
           M NSEEVGKSSI     EE  ER+PSN ENGDGEDED    VE++EEDEE ED NDEEPP
Sbjct: 1   MANSEEVGKSSI-----EEEVERDPSNFENGDGEDED----VEDEEEDEEYEDGNDEEPP 60

Query: 61  RRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNA 120
           + KP++DASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAIKNA
Sbjct: 61  QEKPVTDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNTKTKDSLIEAEVEAIKNA 120

Query: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGA 180
           STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVV+EVVET NPLSGECGA
Sbjct: 121 STMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVIEVVETGNPLSGECGA 180

Query: 181 YTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVAR 240
           YTKPAARSWTFEGS+KYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKR VTPLVAR
Sbjct: 181 YTKPAARSWTFEGSLKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRLVTPLVAR 240

Query: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNS 300
           LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNS+RRQLGNS
Sbjct: 241 LSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSVRRQLGNS 300

Query: 301 LLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360
           LLSSLKYTFKVDKRNSAVRPT GYAFVSTSQ+GGLAPDHRSLRFVRQEFDLRYAIPFGFD
Sbjct: 301 LLSSLKYTFKVDKRNSAVRPTSGYAFVSTSQVGGLAPDHRSLRFVRQEFDLRYAIPFGFD 360

Query: 361 RAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPT 420
           RAAMNFGVSAG+  PWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGMGPT
Sbjct: 361 RAAMNFGVSAGVXFPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGMGPT 420

Query: 421 EPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480
           EPRREVRDENK EN+DSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL
Sbjct: 421 EPRREVRDENKDENNDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNL 480

Query: 481 AKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 540
           AKLTENEFRSFSFQKF+ETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS
Sbjct: 481 AKLTENEFRSFSFQKFMETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFS 536

Query: 541 ISAPS 546
           ISAPS
Sbjct: 541 ISAPS 536

BLAST of Lag0029779 vs. ExPASy TrEMBL
Match: A0A6J1K1Q1 (sorting and assembly machinery component 50 homolog B-like OS=Cucurbita maxima OX=3661 GN=LOC111489331 PE=4 SV=1)

HSP 1 Score: 986.5 bits (2549), Expect = 4.5e-284
Identity = 503/548 (91.79%), Postives = 529/548 (96.53%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNS--EEERAEREPSNLENGDGEDED-DDEVVEEDEEDEEEEDENDE 60
           M +SEEVG+++IR +S  EEE  EREPSN ENGDGEDE+ +DE  EEDEEDE++ED++DE
Sbjct: 1   MESSEEVGENTIRTSSEEEEEEVEREPSNFENGDGEDEEVEDE--EEDEEDEDDEDDDDE 60

Query: 61  EPPRRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAI 120
           EPPRRKP+ DASRL+AQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAI
Sbjct: 61  EPPRRKPILDASRLIAQRSKLENLVERMRKEKVRLRVHDILIKGNSKTKDSLIEAEVEAI 120

Query: 121 KNASTMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGE 180
           KNASTMQELLEAAGVANA LQRLEIFDSVKITLDSGPPELPGTANVV+EVVE  NPLSGE
Sbjct: 121 KNASTMQELLEAAGVANANLQRLEIFDSVKITLDSGPPELPGTANVVIEVVEAGNPLSGE 180

Query: 181 CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPL 240
           CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVY PRLKR +TPL
Sbjct: 181 CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYLPRLKRLMTPL 240

Query: 241 VARLSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQL 300
           VARLSLLSQDW+EFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNSIRRQL
Sbjct: 241 VARLSLLSQDWMEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSIRRQL 300

Query: 301 GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF 360
           GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF
Sbjct: 301 GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF 360

Query: 361 GFDRAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGM 420
           GFDRAAMNFGVSAG+VLPWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGM
Sbjct: 361 GFDRAAMNFGVSAGVVLPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGM 420

Query: 421 GPTEPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGA 480
           GPTEPRREVRDENKG+++D+LGRDFVGGDLAVTAFADLSFDLP+RWLREHGIHGHIFAGA
Sbjct: 421 GPTEPRREVRDENKGKDNDALGRDFVGGDLAVTAFADLSFDLPLRWLREHGIHGHIFAGA 480

Query: 481 GNLAKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGF 540
           GNLAKLTENEFRSFSFQKF+ETFRTSVGVG+VVPTRLFRLEGNFYYILKQQEHDRGKTGF
Sbjct: 481 GNLAKLTENEFRSFSFQKFMETFRTSVGVGIVVPTRLFRLEGNFYYILKQQEHDRGKTGF 540

Query: 541 RFSISAPS 546
           RFSISAPS
Sbjct: 541 RFSISAPS 546

BLAST of Lag0029779 vs. ExPASy TrEMBL
Match: A0A6J1FH45 (sorting and assembly machinery component 50 homolog B-like OS=Cucurbita moschata OX=3662 GN=LOC111445341 PE=4 SV=1)

HSP 1 Score: 985.3 bits (2546), Expect = 1.0e-283
Identity = 504/548 (91.97%), Postives = 527/548 (96.17%), Query Frame = 0

Query: 1   MTNSEEVGKSSIRPNS--EEERAEREPSNLENGDGEDED-DDEVVEEDEEDEEEEDENDE 60
           M NSEEVGK++IR +S  EEE  EREPSN ENGD EDE+ +DE  EEDEEDE++ED+++E
Sbjct: 1   MENSEEVGKNTIRTSSEEEEEEVEREPSNFENGDEEDEEVEDE--EEDEEDEDDEDDDEE 60

Query: 61  EPPRRKPLSDASRLLAQRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAI 120
           EPPRRKP+ DASRL+AQRSKLENLVERMRKEKVRLRVHDILIKGN KTKDSLIEAEVEAI
Sbjct: 61  EPPRRKPILDASRLIAQRSKLENLVERMRKEKVRLRVHDILIKGNSKTKDSLIEAEVEAI 120

Query: 121 KNASTMQELLEAAGVANAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGE 180
           KNASTMQELLEAAGVANA LQRLEIFDSVKITLDSGPPELPGTANVV+EVVE  NPLSGE
Sbjct: 121 KNASTMQELLEAAGVANANLQRLEIFDSVKITLDSGPPELPGTANVVIEVVEAGNPLSGE 180

Query: 181 CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPL 240
           CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVY PRLKR +TPL
Sbjct: 181 CGAYTKPAARSWTFEGSVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYLPRLKRLMTPL 240

Query: 241 VARLSLLSQDWLEFSSYKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQL 300
           VARLSLLSQDW+EFSSYKERSLGLSLGLYSTKYHDL YNLGWRTITDPSQMASNSIRRQL
Sbjct: 241 VARLSLLSQDWMEFSSYKERSLGLSLGLYSTKYHDLGYNLGWRTITDPSQMASNSIRRQL 300

Query: 301 GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF 360
           GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF
Sbjct: 301 GNSLLSSLKYTFKVDKRNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPF 360

Query: 361 GFDRAAMNFGVSAGLVLPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGM 420
           GFDRAAMNFGVSAG+VLPWGNGF+NKPSSLPERFFLGGDFSPVCT+GGP TVWGFKTRGM
Sbjct: 361 GFDRAAMNFGVSAGVVLPWGNGFLNKPSSLPERFFLGGDFSPVCTIGGPTTVWGFKTRGM 420

Query: 421 GPTEPRREVRDENKGENSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGA 480
           GPTEPRREVRDENKGE++D+LGRDFVGGDLAVTAFADLSFDLP+RWLREHGIHGHIFAGA
Sbjct: 421 GPTEPRREVRDENKGEDNDALGRDFVGGDLAVTAFADLSFDLPLRWLREHGIHGHIFAGA 480

Query: 481 GNLAKLTENEFRSFSFQKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGF 540
           GNLAKLTENEFRSFSFQKF ETFRTSVGVG+VVPTRLFRLEGNFYYILKQQEHDRGKTGF
Sbjct: 481 GNLAKLTENEFRSFSFQKFRETFRTSVGVGIVVPTRLFRLEGNFYYILKQQEHDRGKTGF 540

Query: 541 RFSISAPS 546
           RFSISAPS
Sbjct: 541 RFSISAPS 546

BLAST of Lag0029779 vs. TAIR 10
Match: AT5G05520.1 (Outer membrane OMP85 family protein )

HSP 1 Score: 639.0 bits (1647), Expect = 3.4e-183
Identity = 319/531 (60.08%), Postives = 419/531 (78.91%), Query Frame = 0

Query: 18  EERAEREPSNLENGDGEDEDDDEV---VEEDEEDEEEEDENDEEPPRRKPLSDASRLLAQ 77
           E  AE+   N      E ED+ E    +  DEE+EEE +E D+  PR +   DA   +A 
Sbjct: 2   ENPAEKPDPNPSKPKIESEDEREELGDINGDEEEEEEYEEEDDGKPRTR--EDA---IAD 61

Query: 78  RSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNASTMQELLEAAGVAN 137
           R K E+L  RMR   V +RVHD+++KGN+KTKD +IEAEV+ ++ A+T+QELL+A+ VAN
Sbjct: 62  RIKAESLFRRMRATPVAVRVHDVIVKGNEKTKDHVIEAEVDVVRQATTLQELLKASKVAN 121

Query: 138 AKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGAYTKPAARSWTFEGS 197
             LQ L+IFDSVKITLDSGPPELPGT NVV++VVE+ +P++G+ G +TK  ARS + EGS
Sbjct: 122 FNLQALDIFDSVKITLDSGPPELPGTTNVVIDVVESKSPITGQIGTFTKAEARSSSLEGS 181

Query: 198 VKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVARLSLLSQDWLEFSSY 257
           +KYKN  GYGD+WDGSLAYG + ++EV  G+Y PR +   TP  +R+ L +QDWL+FSSY
Sbjct: 182 LKYKNIFGYGDIWDGSLAYGCDHSAEVGLGMYLPRFRGRPTPFTSRVYLSTQDWLKFSSY 241

Query: 258 KERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNSLLSSLKYTFKVDKR 317
           KER+LGLSLGL ++KYH+L YN+ WR + DPSQMAS SIRRQLG++L+S+LKYTFK D+R
Sbjct: 242 KERALGLSLGLIASKYHELAYNIAWRNLIDPSQMASRSIRRQLGHNLVSALKYTFKFDQR 301

Query: 318 NSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFDRAAMNFGVSAGLVL 377
           NS++RPTRGY+F+STSQIGGLAPD R+LRF+RQE DLRYA+P GF RAA+NFG++ G+  
Sbjct: 302 NSSLRPTRGYSFISTSQIGGLAPDSRTLRFLRQEIDLRYAVPLGFYRAALNFGIAGGITF 361

Query: 378 PWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPTEPRREVRDENKGEN 437
           PWG+G+ ++ S +PERFFLGG+ SPVC++GGP+ +WGFKTRG+GP EPRREV+D+   E+
Sbjct: 362 PWGSGYKSRASCVPERFFLGGNISPVCSLGGPSALWGFKTRGLGPNEPRREVQDD---ES 421

Query: 438 SDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNLAKLTENEFRSFSFQ 497
            D+  RDFVGGD+AVTAFADLSFD P++W R+ GIHGH+FA AGN+A+L+EN++R+F+  
Sbjct: 422 GDTYERDFVGGDVAVTAFADLSFDFPLKWFRDRGIHGHVFACAGNMAELSENKYRNFTAP 481

Query: 498 KFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFSISAPS 546
           K LETFR+SVG G+VVPT LFR+E N+ +ILK+QEHDR K+GF  + S  S
Sbjct: 482 KLLETFRSSVGAGIVVPTSLFRMELNYCHILKKQEHDRAKSGFFMTFSTSS 524

BLAST of Lag0029779 vs. TAIR 10
Match: AT3G11070.1 (Outer membrane OMP85 family protein )

HSP 1 Score: 631.3 bits (1627), Expect = 7.1e-181
Identity = 318/532 (59.77%), Postives = 411/532 (77.26%), Query Frame = 0

Query: 14  PNSEEERAEREPSNLENGDGEDEDDDEVVEEDEEDEEEEDENDEEPPRRKPLSDASRLLA 73
           P   E  AE E   L   +G+D  +D        D+ E+D+ + +   R+  S   R+  
Sbjct: 12  PQIREPEAEEEVEELNGYEGDDGGED--------DDGEDDDTESKSQTRESGSSVDRI-- 71

Query: 74  QRSKLENLVERMRKEKVRLRVHDILIKGNKKTKDSLIEAEVEAIKNASTMQELLEAAGVA 133
              K E+L  RMR   V +RVHD++I GN+KTKD +IEAEV+A++ A+T+QELLEA+ VA
Sbjct: 72  ---KAESLFRRMRAAPVPVRVHDVIIGGNEKTKDHIIEAEVDAVREATTLQELLEASRVA 131

Query: 134 NAKLQRLEIFDSVKITLDSGPPELPGTANVVVEVVETSNPLSGECGAYTKPAARSWTFEG 193
           N+ L+ L+IFDSV ITLDSGPPELPGT NVV++VVE+ +PL+G+ GAYT+  ARS + E 
Sbjct: 132 NSNLRALDIFDSVNITLDSGPPELPGTTNVVIDVVESKSPLTGQIGAYTRAEARSSSVEA 191

Query: 194 SVKYKNWLGYGDLWDGSLAYGPNQTSEVSAGVYFPRLKRFVTPLVARLSLLSQDWLEFSS 253
           S+KYKN  GYGD+WDGS+ YG + ++EV  G+Y PR +   TP  +RL L +QDWL+FSS
Sbjct: 192 SLKYKNIFGYGDIWDGSIVYGCDNSAEVGLGMYLPRFRGLSTPFTSRLFLSTQDWLKFSS 251

Query: 254 YKERSLGLSLGLYSTKYHDLVYNLGWRTITDPSQMASNSIRRQLGNSLLSSLKYTFKVDK 313
           YKERSLGLSLGL+S+KYH+L+Y + WR + DPSQ A+ SIRRQLG+SLLS+LKYTF+ D+
Sbjct: 252 YKERSLGLSLGLFSSKYHELIYTIAWRNLIDPSQAAAVSIRRQLGHSLLSALKYTFRFDQ 311

Query: 314 RNSAVRPTRGYAFVSTSQIGGLAPDHRSLRFVRQEFDLRYAIPFGFDRAAMNFGVSAGLV 373
           RNS++RPT GYAF+STSQIGGLAPD RSLRF++QE DLRYA+PFGF  AA+NFGVS G+ 
Sbjct: 312 RNSSLRPTNGYAFISTSQIGGLAPDSRSLRFLKQEVDLRYAVPFGFYNAAINFGVSGGVS 371

Query: 374 LPWGNGFMNKPSSLPERFFLGGDFSPVCTMGGPATVWGFKTRGMGPTEPRREVRDENKGE 433
            PWG+G+ N+PSS+PERFFLGG+ SPVC++ GP+ +WGFKTRG+GP+EP+R      KG+
Sbjct: 372 FPWGSGYQNRPSSIPERFFLGGNSSPVCSLSGPSALWGFKTRGVGPSEPKR------KGD 431

Query: 434 NSDSLGRDFVGGDLAVTAFADLSFDLPIRWLREHGIHGHIFAGAGNLAKLTENEFRSFSF 493
           +     RDFVGGD AVTAFADLSFDLP+RWLRE GIHGH+FA AGN+AKL+ENEFR+F+ 
Sbjct: 432 SE----RDFVGGDAAVTAFADLSFDLPVRWLRERGIHGHVFACAGNMAKLSENEFRNFTA 491

Query: 494 QKFLETFRTSVGVGVVVPTRLFRLEGNFYYILKQQEHDRGKTGFRFSISAPS 546
            KFLETFRTSVG G+V+PT LFR+E N+ +ILK+QEHD+ ++G   + SA S
Sbjct: 492 PKFLETFRTSVGAGIVLPTSLFRMELNYCHILKKQEHDQARSGVFLTFSASS 520

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038885406.11.9e-29294.50sorting and assembly machinery component 50 homolog [Benincasa hispida] >XP_0388... [more]
KAA0036493.11.7e-28592.84sorting and assembly machinery component 50-like protein [Cucumis melo var. maku... [more]
XP_008456518.16.4e-28592.66PREDICTED: LOW QUALITY PROTEIN: sorting and assembly machinery component 50 homo... [more]
XP_004140074.16.4e-28593.03sorting and assembly machinery component 50 homolog [Cucumis sativus] >KGN47843.... [more]
KAG6578810.18.4e-28592.15Sorting and assembly machinery component 50-like protein, partial [Cucurbita arg... [more]
Match NameE-valueIdentityDescription
Q8BGH23.2e-2928.45Sorting and assembly machinery component 50 homolog OS=Mus musculus OX=10090 GN=... [more]
Q6PA354.7e-2827.29Sorting and assembly machinery component 50 homolog B OS=Xenopus laevis OX=8355 ... [more]
Q6P8066.1e-2827.49Sorting and assembly machinery component 50 homolog OS=Xenopus tropicalis OX=836... [more]
Q9Y5121.0e-2727.80Sorting and assembly machinery component 50 homolog OS=Homo sapiens OX=9606 GN=S... [more]
Q5U3I05.2e-2727.21Sorting and assembly machinery component 50 homolog B OS=Danio rerio OX=7955 GN=... [more]
Match NameE-valueIdentityDescription
A0A5A7T0T18.2e-28692.84Sorting and assembly machinery component 50-like protein OS=Cucumis melo var. ma... [more]
A0A0A0KE643.1e-28593.03Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G406550 PE=4 SV=1[more]
A0A1S3C3103.1e-28592.66LOW QUALITY PROTEIN: sorting and assembly machinery component 50 homolog OS=Cucu... [more]
A0A6J1K1Q14.5e-28491.79sorting and assembly machinery component 50 homolog B-like OS=Cucurbita maxima O... [more]
A0A6J1FH451.0e-28391.97sorting and assembly machinery component 50 homolog B-like OS=Cucurbita moschata... [more]
Match NameE-valueIdentityDescription
AT5G05520.13.4e-18360.08Outer membrane OMP85 family protein [more]
AT3G11070.17.1e-18159.77Outer membrane OMP85 family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 68..95
NoneNo IPR availableGENE3D3.10.20.310membrane protein fhaccoord: 90..170
e-value: 3.4E-8
score: 35.7
NoneNo IPR availableGENE3D2.40.160.50membrane protein fhac: a member of the omp85/tpsb transporter familycoord: 183..544
e-value: 1.3E-41
score: 145.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 11..28
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 413..436
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 418..436
NoneNo IPR availablePANTHERPTHR12815:SF38OUTER MEMBRANE OMP85 FAMILY PROTEINcoord: 23..540
IPR010827POTRA domain, BamA/TamA-likePFAMPF07244POTRAcoord: 93..169
e-value: 4.3E-5
score: 24.0
IPR000184Bacterial surface antigen (D15)PFAMPF01103Omp85coord: 199..542
e-value: 4.8E-28
score: 98.6
IPR039910Surface antigen D15-likePANTHERPTHR12815SORTING AND ASSEMBLY MACHINERY SAMM50 PROTEIN FAMILY MEMBERcoord: 23..540
IPR034746POTRA domainPROSITEPS51779POTRAcoord: 92..170
score: 9.254827

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0029779.1Lag0029779.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034622 cellular protein-containing complex assembly
biological_process GO:0045040 protein insertion into mitochondrial outer membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0009527 plastid outer membrane
cellular_component GO:0001401 SAM complex
cellular_component GO:0019867 outer membrane