Lag0028276 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0028276
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2-isopropylmalate synthase
Location	chr8: 17508829 .. 17509385 (-)
RNA-Seq Expression	Lag0028276
Synteny	Lag0028276

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGCCAAAGGTAGTGTGACTGAGTTGTTTCCTATGTGTACGTTCCCAAAGATGTACTGGAATGATTTCGGATATACAAAACTTTGAATGGAAGACCAACCAATAATGCTTCAAAAGAGTTACAGTTGATGGTGGTCAAATAGAGTCTAGTACTTGTTCTAGTAGAGGTTCTAAGAAGTTCAATTTAAGTGATGAAGATTGAAAAGACTAAATTGAAAGAGCGTCCAAACATTAGAGACTAAAACATATCTACGTTACAACTTGGAACATTTCCAATAATATATACATACATATATAAATAATTATGTATTTTGTTTTGTGGTGTCCAATCAGATCTGATAAAGAGTTCCTTTATGAAATTCTGGGTGAAGTTATAGAAGCAGGGGCCACTACTCTCAACATAACAGATACAGTTGGTTACACTGTGCCAGTTGAATATGGGAATTTGATTGCTGATATAAAATCTTATACTCCAGGCAGTGAAAATGTTATCATTTCAACACATTGCCAGAATGATCTTGGACTTGCAACTGCCAACACAATAGCCGTGAGTTAA

mRNA sequence

ATGAGCCAAAGATCTGATAAAGAGTTCCTTTATGAAATTCTGGGTGAAGTTATAGAAGCAGGGGCCACTACTCTCAACATAACAGATACAGTTGGTTACACTGTGCCAGTTGAATATGGGAATTTGATTGCTGATATAAAATCTTATACTCCAGGCAGTGAAAATGTTATCATTTCAACACATTGCCAGAATGATCTTGGACTTGCAACTGCCAACACAATAGCCGTGAGTTAA

Coding sequence (CDS)

ATGAGCCAAAGATCTGATAAAGAGTTCCTTTATGAAATTCTGGGTGAAGTTATAGAAGCAGGGGCCACTACTCTCAACATAACAGATACAGTTGGTTACACTGTGCCAGTTGAATATGGGAATTTGATTGCTGATATAAAATCTTATACTCCAGGCAGTGAAAATGTTATCATTTCAACACATTGCCAGAATGATCTTGGACTTGCAACTGCCAACACAATAGCCGTGAGTTAA

Protein sequence

MSQRSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQNDLGLATANTIAVS

Homology

[Close]

BLAST of Lag0028276 vs. NCBI nr
Match: XP_022934697.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 130.2 bits (326), Expect = 7.7e-27
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSDKEFLY+ILGEVI AGATTLNITDTVGYTVP EYG LIADIK  TPG++NVIISTHCQ
Sbjct: 203 RSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQ 262

Query: 64  NDLGLATANTIA 76
           NDLGLATANTIA
Sbjct: 263 NDLGLATANTIA 274

[Close]

BLAST of Lag0028276 vs. NCBI nr
Match: XP_023521686.1 (2-isopropylmalate synthase 2, chloroplastic-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 130.2 bits (326), Expect = 7.7e-27
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSDKEFLY+ILGEVI AGATTLNITDTVGYTVP EYG LIADIK  TPG++NVIISTHCQ
Sbjct: 201 RSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQ 260

Query: 64  NDLGLATANTIA 76
           NDLGLATANTIA
Sbjct: 261 NDLGLATANTIA 272

[Close]

BLAST of Lag0028276 vs. NCBI nr
Match: XP_023528730.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023528731.1 2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 130.2 bits (326), Expect = 7.7e-27
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSDKEFLY+ILGEVI AGATTLNITDTVGYTVP EYG LIADIK  TPG++NVIISTHCQ
Sbjct: 201 RSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQ 260

Query: 64  NDLGLATANTIA 76
           NDLGLATANTIA
Sbjct: 261 NDLGLATANTIA 272

[Close]

BLAST of Lag0028276 vs. NCBI nr
Match: XP_022934698.1 (2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 130.2 bits (326), Expect = 7.7e-27
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSDKEFLY+ILGEVI AGATTLNITDTVGYTVP EYG LIADIK  TPG++NVIISTHCQ
Sbjct: 203 RSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQ 262

Query: 64  NDLGLATANTIA 76
           NDLGLATANTIA
Sbjct: 263 NDLGLATANTIA 274

[Close]

BLAST of Lag0028276 vs. NCBI nr
Match: KAG6580949.1 (2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 130.2 bits (326), Expect = 7.7e-27
Identity = 64/72 (88.89%), Postives = 67/72 (93.06%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSDKEFLY+ILGEVI AGATTLNITDTVGYTVP EYG LIADIK  TPG++NVIISTHCQ
Sbjct: 196 RSDKEFLYQILGEVIRAGATTLNITDTVGYTVPAEYGKLIADIKCNTPGTQNVIISTHCQ 255

Query: 64  NDLGLATANTIA 76
           NDLGLATANTIA
Sbjct: 256 NDLGLATANTIA 267

[Close]

BLAST of Lag0028276 vs. ExPASy Swiss-Prot
Match: O04973 (2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 2.8e-27
Identity = 59/72 (81.94%), Postives = 66/72 (91.67%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSD+EFLY+ILGEVI+AGATTLNI DTVGYTVP E+G LI DIK+ TPG ENVIISTHCQ
Sbjct: 197 RSDREFLYDILGEVIKAGATTLNIPDTVGYTVPSEFGQLITDIKANTPGIENVIISTHCQ 256

Query: 64  NDLGLATANTIA 76
           NDLGL+TANT+A
Sbjct: 257 NDLGLSTANTLA 268

[Close]

BLAST of Lag0028276 vs. ExPASy Swiss-Prot
Match: O04974 (2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 8.9e-26
Identity = 58/72 (80.56%), Postives = 64/72 (88.89%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSD EFLY ILGEVI+AGATTLNI DTVGYTVP E+G LIA IK+ TPG E+VIISTHCQ
Sbjct: 220 RSDPEFLYHILGEVIKAGATTLNIPDTVGYTVPEEFGQLIAKIKANTPGVEDVIISTHCQ 279

Query: 64  NDLGLATANTIA 76
           NDLGL+TANT+A
Sbjct: 280 NDLGLSTANTLA 291

[Close]

BLAST of Lag0028276 vs. ExPASy Swiss-Prot
Match: Q9C550 (2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS2 PE=1 SV=1)

HSP 1 Score: 115.5 bits (288), Expect = 2.6e-25
Identity = 55/72 (76.39%), Postives = 66/72 (91.67%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RS++E+LYEILGEVI+AGATTLNI DTVG T+P E+G LIADIK+ TPG +NVIISTHCQ
Sbjct: 236 RSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLIADIKANTPGIQNVIISTHCQ 295

Query: 64  NDLGLATANTIA 76
           NDLGL+TANT++
Sbjct: 296 NDLGLSTANTLS 307

[Close]

BLAST of Lag0028276 vs. ExPASy Swiss-Prot
Match: Q9LPR4 (2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IPMS1 PE=1 SV=2)

HSP 1 Score: 114.0 bits (284), Expect = 7.5e-25
Identity = 53/72 (73.61%), Postives = 65/72 (90.28%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RS++E+LYEILGEVI+AGATTLNI DTVG T+P E+G LI D+K+ TPG ENV+ISTHCQ
Sbjct: 238 RSEREYLYEILGEVIKAGATTLNIPDTVGITLPSEFGQLITDLKANTPGIENVVISTHCQ 297

Query: 64  NDLGLATANTIA 76
           NDLGL+TANT++
Sbjct: 298 NDLGLSTANTLS 309

[Close]

BLAST of Lag0028276 vs. ExPASy Swiss-Prot
Match: Q39891 (Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 8.3e-24
Identity = 55/71 (77.46%), Postives = 59/71 (83.10%), Query Frame = 0


Query: 4   RSDKEFLYEILGEVIEAGATTLNITDTVGYTVPVEYGNLIADIKSYTPGSENVIISTHCQ 63
           RSD+EFLYEILG VIEAGATT+NI DTVG  +P+E G LI DIK  TPG  NVIISTHC 
Sbjct: 182 RSDREFLYEILGVVIEAGATTVNIADTVGIVMPLELGKLIVDIKDNTPGIANVIISTHCH 241

Query: 64  NDLGLATANTI 75
           NDLGLATANTI
Sbjct: 242 NDLGLATANTI 252

The following BLAST results are available for this feature:

BLAST of Lag0028276 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022934697.1	7.7e-27	88.89	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
XP_023521686.1	7.7e-27	88.89	2-isopropylmalate synthase 2, chloroplastic-like, partial [Cucurbita pepo subsp....	[more]
XP_023528730.1	7.7e-27	88.89	2-isopropylmalate synthase 2, chloroplastic-like isoform X1 [Cucurbita pepo subs...	[more]
XP_022934698.1	7.7e-27	88.89	2-isopropylmalate synthase 2, chloroplastic-like isoform X2 [Cucurbita moschata]	[more]
KAG6580949.1	7.7e-27	88.89	2-isopropylmalate synthase 2, chloroplastic, partial [Cucurbita argyrosperma sub...	[more]

BLAST of Lag0028276 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04973	2.8e-27	81.94	2-isopropylmalate synthase A OS=Solanum pennellii OX=28526 GN=IPMSA PE=2 SV=1	[more]
O04974	8.9e-26	80.56	2-isopropylmalate synthase B OS=Solanum pennellii OX=28526 GN=IPMSB PE=2 SV=1	[more]
Q9C550	2.6e-25	76.39	2-isopropylmalate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q9LPR4	7.5e-25	73.61	2-isopropylmalate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=I...	[more]
Q39891	8.3e-24	77.46	Probable 2-isopropylmalate synthase OS=Glycine max OX=3847 GN=GMN56 PE=2 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000891	Pyruvate carboxyltransferase	PFAM	PF00682	HMGL-like	coord: 1..75 e-value: 1.1E-20 score: 74.3
IPR000891	Pyruvate carboxyltransferase	PROSITE	PS50991	PYR_CT	coord: 1..77 score: 21.770641
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 1..76 e-value: 1.1E-21 score: 79.1
None	No IPR available	PANTHER	PTHR10277:SF64	2-ISOPROPYLMALATE SYNTHASE 1 CHLOROPLASTIC	coord: 3..75
None	No IPR available	PANTHER	PTHR10277	HOMOCITRATE SYNTHASE-RELATED	coord: 3..75
None	No IPR available	SUPERFAMILY	51569	Aldolase	coord: 3..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0028276.1	Lag0028276.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009098	leucine biosynthetic process
molecular_function	GO:0003852	2-isopropylmalate synthase activity
molecular_function	GO:0003824	catalytic activity