Lag0027957 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027957
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr8: 8698706 .. 8700189 (+)
RNA-Seq ExpressionLag0027957
SyntenyLag0027957
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGGAGAAGGTGGACAAAGAGTTCTGGTGTCTTGTGCTCTATGAACAGTTACCAAACTTCTGTTATGAATGTGGTTGTCTGGGGCATTCGAGAAGGGAGTGTGGGTCTATTGAGGGCGGGTCACTGGCTCAGGGGGTGAAAAGCATGGGGAATGACTGAGAGTTGAGGCGGTCTATGGTGAGGGGAGCAAGCGGGGTCAGGAGGGGAAGGTGAAGAGCAGAGGGGAGCAGAGTGAGGATGTTCCTATATCTCATTCTATTGCGCAGGATCCTCAAAATATTGGCATAATAGAAGAAGCGATTAATAAGGTGAGTGCTTCGAGGGATGCGTCGGGGGTCCAAGTGGGTGAGCATTGCGATATAACTGTGATTGATCCACGACCTGAGGAGAGGGCGGACGCTGGGGTGAGTGGGAATGGAGGGGGAAGGGTGGATAATGGGAAAGGGAAGCAGAAAGTGGGTGACGCTTGGGGTGAGTCCTCGAGTGTACTTATGGGGGAGGAGGCGATGGTGGTGGATTCTATAGATGACGGGCAGACTAAAGGGGTGGAGAAGGCGCTGAAAGTGAATGCGAGTAGAGGTTGGAAGAGAAGGGCAAGGGATGCAATGGCTGATGTTATAAATAGAGAGGCTTCTGTTGAGGTAGGGGCGGGGAAGAGGAAGGCGGACGTAGGTGAGGGTGATGGGCAGTTGGGGAAGAAGGCAAGGAGGGAGGGGTTGTGGAGCTATCTATTGGGGGGGTTGGTGTTGTTGTGTTGGCGGAGGCTGGGACCCAGCCTCGCCAGTCCCCATGAGTGTCCTACTTTTGAATGTACGAGGCTTGGGGTCGGCTCGTGCATTCCGGCGGTTGTACAAGTTGATACAACAACACAGACCTCACATGGTGTTCCTGTCTGAAACCAAGGTTCACTCGTTGCGGTTTGATTCAATAAAAGTCAGGTTGGGCTTTTCGGGTTGCTTATCTGTTGATTGCGTTGGCAGGAGTGGTGATTTGGCGTTGTTATGGAGTTCAGAAATTCAGTTTAGTCTCATCTCATTCACCTCTAATCACATTGATGGCTGGGTAGATGAGAGGTCAAGGCAATGGAGATTTTTTGGGATCTATGGGCATCCACAAGCGAAGTTGAAGACAAGAACATGGGCATTGATGAAACACCTTCGTGGGAGATCGGTAGCACCATGGTTAGTTGGGGGTGACTTTAATGTGATCCTATTTCACCATGAAAAGGATGGGGGTAGGTTGAAACCAGAGGCAGGATTGGTTGGGTTTCACGAGGCGATGGATGTTTGTGATTTAATGGATCTGGGTTTCAGAGGAGAGGCATTTACCTGGTGCATGATGACATGCAAAAATGTCATCATTTTCCTATTAATTTTAGCTAGTTTTGTGCATGAATTGTGAGCTTTAATGTATTTTTATGTTGATTTTACAGGAAACCGAGTTCGGACAGCAAAACTGAGATTTTAAGAGAATTTGGGTGA

mRNA sequence

ATGCAGGAGAAGGTGGACAAAGAGTTCTGGTGTCTTGTGCTCTATGAACAGTTACCAAACTTCTGTTATGAATGTGGTTGTCTGGGGCATTCGAGAAGGGAGTGTGGGTCTATTGAGGGCGGGTCACTGGCTCAGGGGGATCCTCAAAATATTGGCATAATAGAAGAAGCGATTAATAAGGTGAGTGCTTCGAGGGATGCGTCGGGGGTCCAAGTGGGTGAGCATTGCGATATAACTGTGATTGATCCACGACCTGAGGAGAGGGCGGACGCTGGGGTGAGTGGGAATGGAGGGGGAAGGGTGGATAATGGGAAAGGGAAGCAGAAAGTGGGTGACGCTTGGGGTGAGTCCTCGAGTGTACTTATGGGGGAGGAGGCGATGGTGGTGGATTCTATAGATGACGGGCAGACTAAAGGGGTGGAGAAGGCGCTGAAAGTGAATGCGAGTAGAGGTTGGAAGAGAAGGGCAAGGGATGCAATGGCTGATGTTATAAATAGAGAGGCTTCTGTTGAGGTAGGGGCGGGGAAGAGGAAGGCGGACGTAGCCTCGCCAGTCCCCATGAGTGTCCTACTTTTGAATGTACGAGGCTTGGGGTCGGCTCGTGCATTCCGGCGGTTGTACAAGTTGATACAACAACACAGACCTCACATGGTGTTCCTGTCTGAAACCAAGGTTCACTCGTTGCGGTTTGATTCAATAAAAGTCAGGTTGGGCTTTTCGGGTTGCTTATCTGTTGATTGCGTTGGCAGGAGTGGTGATTTGGCGTTGTTATGGAGTTCAGAAATTCAGTTTAGTCTCATCTCATTCACCTCTAATCACATTGATGGCTGGGTAGATGAGAGGTCAAGGCAATGGAGATTTTTTGGGATCTATGGGCATCCACAAGCGAAGTTGAAGACAAGAACATGGGCATTGATGAAACACCTTCGTGGGAGATCGGTAGCACCATGGTTAGTTGGGGGTGACTTTAATGTGATCCTATTTCACCATGAAAAGGATGGGGGTAGGTTGAAACCAGAGGCAGGATTGGTTGGGTTTCACGAGGCGATGGATGTTTGTGATTTAATGGATCTGGGTTTCAGAGGAGAGGCATTTACCTGGAAACCGAGTTCGGACAGCAAAACTGAGATTTTAAGAGAATTTGGGTGA

Coding sequence (CDS)

ATGCAGGAGAAGGTGGACAAAGAGTTCTGGTGTCTTGTGCTCTATGAACAGTTACCAAACTTCTGTTATGAATGTGGTTGTCTGGGGCATTCGAGAAGGGAGTGTGGGTCTATTGAGGGCGGGTCACTGGCTCAGGGGGATCCTCAAAATATTGGCATAATAGAAGAAGCGATTAATAAGGTGAGTGCTTCGAGGGATGCGTCGGGGGTCCAAGTGGGTGAGCATTGCGATATAACTGTGATTGATCCACGACCTGAGGAGAGGGCGGACGCTGGGGTGAGTGGGAATGGAGGGGGAAGGGTGGATAATGGGAAAGGGAAGCAGAAAGTGGGTGACGCTTGGGGTGAGTCCTCGAGTGTACTTATGGGGGAGGAGGCGATGGTGGTGGATTCTATAGATGACGGGCAGACTAAAGGGGTGGAGAAGGCGCTGAAAGTGAATGCGAGTAGAGGTTGGAAGAGAAGGGCAAGGGATGCAATGGCTGATGTTATAAATAGAGAGGCTTCTGTTGAGGTAGGGGCGGGGAAGAGGAAGGCGGACGTAGCCTCGCCAGTCCCCATGAGTGTCCTACTTTTGAATGTACGAGGCTTGGGGTCGGCTCGTGCATTCCGGCGGTTGTACAAGTTGATACAACAACACAGACCTCACATGGTGTTCCTGTCTGAAACCAAGGTTCACTCGTTGCGGTTTGATTCAATAAAAGTCAGGTTGGGCTTTTCGGGTTGCTTATCTGTTGATTGCGTTGGCAGGAGTGGTGATTTGGCGTTGTTATGGAGTTCAGAAATTCAGTTTAGTCTCATCTCATTCACCTCTAATCACATTGATGGCTGGGTAGATGAGAGGTCAAGGCAATGGAGATTTTTTGGGATCTATGGGCATCCACAAGCGAAGTTGAAGACAAGAACATGGGCATTGATGAAACACCTTCGTGGGAGATCGGTAGCACCATGGTTAGTTGGGGGTGACTTTAATGTGATCCTATTTCACCATGAAAAGGATGGGGGTAGGTTGAAACCAGAGGCAGGATTGGTTGGGTTTCACGAGGCGATGGATGTTTGTGATTTAATGGATCTGGGTTTCAGAGGAGAGGCATTTACCTGGAAACCGAGTTCGGACAGCAAAACTGAGATTTTAAGAGAATTTGGGTGA

Protein sequence

MQEKVDKEFWCLVLYEQLPNFCYECGCLGHSRRECGSIEGGSLAQGDPQNIGIIEEAINKVSASRDASGVQVGEHCDITVIDPRPEERADAGVSGNGGGRVDNGKGKQKVGDAWGESSSVLMGEEAMVVDSIDDGQTKGVEKALKVNASRGWKRRARDAMADVINREASVEVGAGKRKADVASPVPMSVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGCLSVDCVGRSGDLALLWSSEIQFSLISFTSNHIDGWVDERSRQWRFFGIYGHPQAKLKTRTWALMKHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRGEAFTWKPSSDSKTEILREFG
Homology
BLAST of Lag0027957 vs. NCBI nr
Match: XP_018826186.1 (uncharacterized protein LOC108995141 [Juglans regia] >KAF5471486.1 hypothetical protein F2P56_008274 [Juglans regia])

HSP 1 Score: 179.9 bits (455), Expect = 4.2e-41
Identity = 115/385 (29.87%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 9   FWCLVLYEQLPNFCYECGCLGHSRRECGSIEGGSLAQGD--PQNIGIIEEAINKVSASRD 68
           FW    YE+LP  C+ CGC+ H    C   +G +    D   +     E    +  A   
Sbjct: 193 FWAPFSYEKLPKICFSCGCMVHGVNGCSGGQGNNEEMEDKWKRESEKKENLETRAGAKEG 252

Query: 69  ASGVQVGEHCDITVIDPRPEERADAG--VSGNGGGRVDNG---KGKQKVGDAWGESSSVL 128
            +G+   E    T    +  E ++ G  +    G R D G   +G+++      E  S  
Sbjct: 253 KNGLGSDEDEGQTRKKEKGREESEGGKDLEEENGDREDEGMMRRGEERWDKQATEEESEN 312

Query: 129 MGEEAM------VVDSIDDGQTK-GVEKALKVNASRGWKRRARDAMADVINREASVEVGA 188
           M ++A+      +V+   +G+   GVE   K      WKRRAR     V++         
Sbjct: 313 MVDKALEETYGQMVEVSSEGKGGIGVEDHPKKKGE--WKRRARAKGKQVVS--------- 372

Query: 189 GKRKADVASPVP---MSVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFD 248
                    P+P   M +L  N RGLG+ R  + LY ++++++P +VF+ ETK     FD
Sbjct: 373 -------DYPIPPNIMKILSWNCRGLGNPRTVQDLYNMVEKNKPSLVFVMETKSLKKSFD 432

Query: 249 SIKVRLGFSGCLSVDCVGRSGDLALLWSSEIQFSLISFTSNHIDGWVDER-SRQWRFFGI 308
           S++ RL   GC  V+ VG+ G L LLW+S+++  +++++ +HI+ W++E   ++W     
Sbjct: 433 SLRRRLQCEGCFVVEAVGKGGGLVLLWNSDVRAEIVNYSQSHINVWIEEEGEKKWLLTCF 492

Query: 309 YGHPQAKLKTRTWALMKHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAM 368
           YG P    +  +W+L+  L+  +   W + GDFN IL + EK GGR +PE  +  F E M
Sbjct: 493 YGQPDTNKRKESWSLLNSLKPAANIGWCIVGDFNEILTNDEKWGGRARPEGQMELFREVM 552

Query: 369 DVCDLMDLGFRGEAFTWKPSSDSKT 376
           +  +L DLG++G+ FTW  S    T
Sbjct: 553 NEGNLYDLGWKGDKFTWSNSHGDDT 559

BLAST of Lag0027957 vs. NCBI nr
Match: KAF5449841.1 (hypothetical protein F2P56_030246 [Juglans regia])

HSP 1 Score: 178.3 bits (451), Expect = 1.2e-40
Identity = 87/182 (47.80%), Postives = 109/182 (59.89%), Query Frame = 0

Query: 187 MSVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGCLSVD 246
           M +L  N  GLG+ +  R L  LI +  P +VFL ETK+ +    + K + GF  C  VD
Sbjct: 1   MKILCWNSSGLGNPQGIRALRDLITREDPDLVFLQETKMRAQAMTTCKYKFGFVNCFFVD 60

Query: 247 CVGRSGDLALLWSSEIQFSLISFTSNHIDGWVDERS-RQWRFFGIYGHPQAKLKTRTWAL 306
           CVGRSG L+LLW  EI  S+ SF+  HID  + E    +WRF G+YGHP A  +  TW L
Sbjct: 61  CVGRSGGLSLLWKREISVSISSFSRYHIDACIQESDLAEWRFTGVYGHPDASNRYLTWNL 120

Query: 307 MKHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRGEAF 366
           M+ L   +  PWLVGGDFN +L+ HEK GGR  P A L  F   M+ C L DLGF+G  F
Sbjct: 121 MRTLCPSTSGPWLVGGDFNEVLYLHEKRGGRPWPYAQLENFRAVMEDCALQDLGFQGPRF 180

Query: 367 TW 368
           TW
Sbjct: 181 TW 182

BLAST of Lag0027957 vs. NCBI nr
Match: XP_030970584.1 (uncharacterized protein LOC115990959 [Quercus lobata])

HSP 1 Score: 174.1 bits (440), Expect = 2.3e-39
Identity = 84/183 (45.90%), Postives = 114/183 (62.30%), Query Frame = 0

Query: 187 MSVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGCLSVD 246
           M+ L  N RGLG+ R+ R L+ L+Q   P +VFLSETKV   R + IK R+GF+  L V 
Sbjct: 1   MNSLGWNCRGLGNPRSVRALHNLVQYWAPKLVFLSETKVRKKRMERIKERIGFANGLFVP 60

Query: 247 CVGRSGDLALLWSSEIQFSLISFTSNHIDGWVDERSR--QWRFFGIYGHPQAKLKTRTWA 306
           C GRSG LALLW+ E    + SF+++HID  + E +   +WRF G YGHPQ  L+ ++W 
Sbjct: 61  CHGRSGGLALLWTRETDLEIKSFSNHHIDAVITEENSNFKWRFTGFYGHPQTHLRQKSWD 120

Query: 307 LMKHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRGEA 366
           L+  L+G+S  PW   GDFN IL   EK GG ++ +  +  F E ++VC   DLG+ G  
Sbjct: 121 LLDFLQGQSQLPWFCFGDFNEILSMEEKTGGAIRSQGQMDKFREVVNVCGFKDLGYMGPD 180

Query: 367 FTW 368
           FTW
Sbjct: 181 FTW 183

BLAST of Lag0027957 vs. NCBI nr
Match: XP_022841874.1 (uncharacterized protein LOC111365549 [Olea europaea var. sylvestris])

HSP 1 Score: 170.6 bits (431), Expect = 2.6e-38
Identity = 121/425 (28.47%), Postives = 189/425 (44.47%), Query Frame = 0

Query: 7   KEFWCLVLYEQLPNFCYECGCLGHSRRE-------------CGSIEGGSLAQGDPQNIG- 66
           K FW    YE+LPNFCY CG LGH  ++                  G  L  G+  +   
Sbjct: 192 KPFWTRFSYERLPNFCYLCGVLGHGHKDYILWKPAMEAYTTSDYSYGPWLRAGNSSDYSG 251

Query: 67  -IIEEAINK---------VSASRDASGVQVGEHCDITVIDPR-----PEERADAGVSGNG 126
            I++  IN           + S D S   +  H ++  +  +     P  + D  +  N 
Sbjct: 252 PIVDRQINSRTPDSPNSLANPSSDPSSANI-THVNLVNLTNKDQLLEPYGKGDPAMLLN- 311

Query: 127 GGRVDNGKGKQKV---GDAWGESSSVLMGEEAMVVDSIDDG--------------QTKGV 186
              + N +  Q+    G +    ++  +    +  D+I D               QTKG 
Sbjct: 312 ---LTNSQELQQTNFEGISTASRTATNLELTPLQTDAIMDNLTAENTRHSGLADTQTKGT 371

Query: 187 EKALK-----------------VNASRGWKR-RARDAMADVINREASVEVGAGKRKADVA 246
              L+                  N +R WKR     A+  ++    +    +    ++ A
Sbjct: 372 HGLLQTDPVSSNSAEVLTGAKGANFTRRWKRLNTNHAIHSIVPPPQTSLKRSLDFPSENA 431

Query: 247 SPVPMSVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGC 306
            P+P+ +L  N +G  + R    L  LI++  P ++FL ETK+++   +  +++L F GC
Sbjct: 432 PPIPLKLLSWNAQGHRNPRGIHALSNLIRKEDPDVLFLQETKLNAANMELCRIKLKFYGC 491

Query: 307 LSVDCVGRSGDLALLWSSEIQFSLISFTSNHIDGWVDERSRQWRFFGIYGHPQAKLKTRT 366
           L+V  VGRSG +ALLW S +  S++ +++ HID  ++     W   GIYGHP+   +  T
Sbjct: 492 LNVQAVGRSGGIALLWKSNVNLSILGYSTKHIDAKIESPLHCWFLTGIYGHPETTKRMET 551

Query: 367 WALMKHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRG 368
           W L+K L+  S   WLV GDFN IL + EK GGR +P   L  F      C+L DLGF+G
Sbjct: 552 WNLLKRLKRNSSEAWLVFGDFNEILSNEEKWGGRDRPRQQLENFQHMFLECELRDLGFKG 611

BLAST of Lag0027957 vs. NCBI nr
Match: OMO59710.1 (reverse transcriptase [Corchorus capsularis])

HSP 1 Score: 170.2 bits (430), Expect = 3.4e-38
Identity = 85/202 (42.08%), Postives = 113/202 (55.94%), Query Frame = 0

Query: 169 SVEVGAGKRKADVASPVPMSVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSL 228
           S +VG      +V  P  M  +  N RGLG+    R L +LI++ RP++VFL ETK+   
Sbjct: 415 SADVG---ENTNVVPPGAMIGVAWNCRGLGNPWTVRALRELIRRERPNVVFLMETKLPDF 474

Query: 229 RFDSIKVRLGFSGCLSVDCVGRSGDLALLWSSEIQFSLISFTSNHIDGWVDERSR--QWR 288
           + DSI+ R     C  V   GRSG LA+ W   +   LISF+ +HID WVDE  R  +WR
Sbjct: 475 KLDSIRRRCKMHACFGVSATGRSGGLAMFWDDSVDLQLISFSQHHIDMWVDENQRLAKWR 534

Query: 289 FFGIYGHPQAKLKTRTWALMKHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGF 348
             G YG      +  +W L++         W   GDFN +L+  EKDGGR +PEA +V F
Sbjct: 535 LTGFYGEADTGRRHLSWDLLRQFVLHDEKNWFCFGDFNELLWQAEKDGGRERPEAQMVAF 594

Query: 349 HEAMDVCDLMDLGFRGEAFTWK 369
            EA+D C L D+G+RG  FTWK
Sbjct: 595 REALDDCGLYDIGYRGNMFTWK 613

BLAST of Lag0027957 vs. ExPASy TrEMBL
Match: A0A2N9EWI9 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS7087 PE=4 SV=1)

HSP 1 Score: 184.5 bits (467), Expect = 8.3e-43
Identity = 132/425 (31.06%), Postives = 191/425 (44.94%), Query Frame = 0

Query: 7   KEFWCLVLYEQLPNFCYECGCLGHSRREC--------GSIEGGS--LAQGDPQNIGIIEE 66
           K+ W    YE+LP FC+ CG LGH  + C        G ++ G+   A G  + +G    
Sbjct: 193 KKTWVHYRYERLPMFCFRCGVLGHEVQACHQTSLSKEGDLQYGAWLRANGGMKEMGTPGF 252

Query: 67  AINKVSASRDASGVQVGEHCDITVIDPRPEERA-DAGVSGNGGGRVD----NGKGK---Q 126
              + SA  +  G  V +           + RA  A V G    R+D    NGKG+    
Sbjct: 253 PF-RFSARPETLGATVAQSPAGEGGSQGLQPRAIKASVIGGNQSRIDGEESNGKGEYSNS 312

Query: 127 KVGDAWGESSSVLMGEEAM--------------------VVDSIDDGQTKGVEKALKVNA 186
            +        + + G E +                    + D+I +  TK     L+ + 
Sbjct: 313 NLNSPEFNMEAEIRGHEILYENGCVTDKERLANIIQGDYLADTITESPTKNFGHDLRTDV 372

Query: 187 SRG---------------WKRRARDAMADVINREASVEVGAGKRKADVASPVPMSVLLLN 246
             G               WKR   D M DV     +  +G GK  +  A P PM+++  N
Sbjct: 373 ESGGGPAIPSHKPKKPTTWKRIHVDQMPDV-----TYTLGHGKIGSKRAPPSPMNLISWN 432

Query: 247 VRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGCLSVDCVGRSGD 306
            RGLG+ R  R L  L++   P ++FL ETK+ S+  + I+V LG+     V C GR G 
Sbjct: 433 CRGLGNHRTVRALRSLVKTKGPTVLFLIETKLDSVEMEHIRVLLGYVNMFVVPCYGRCGG 492

Query: 307 LALLWSSEIQFSLISFTSNHIDGWVDERS-RQWRFFGIYGHPQAKLKTRTWALMKHLRGR 366
           LAL W  +I   + S++ +HID  V   S  +WR  G YGHP+   ++ +WAL+ HLR R
Sbjct: 493 LALFWGPDIGLEISSYSRHHIDSVVGVGSGYEWRLTGFYGHPEHHKRSESWALLDHLRTR 552

Query: 367 SVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRGEAFTWKPSSD 378
           +V PW   GDFN ILF  EK G   +PE  +  F + +  C L D+GF+G  FTW    D
Sbjct: 553 NVLPWCCVGDFNEILFQTEKQGYHDRPERQIQAFRDVLARCHLTDMGFQGVDFTWDNRRD 611

BLAST of Lag0027957 vs. ExPASy TrEMBL
Match: A0A2N9GJ35 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS27322 PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 2.4e-42
Identity = 117/367 (31.88%), Postives = 170/367 (46.32%), Query Frame = 0

Query: 9   FWCLVLYEQLPNFCYECGCLGHSRRECGSIEGGSLAQGDPQNIGIIEEAINKVSASRDAS 68
           FW    YE LP FCY CG LGH   EC    GG +++G                     S
Sbjct: 198 FWVDFKYEHLPIFCYRCGRLGHGSHECVVGRGGRISEG--------------------VS 257

Query: 69  GVQVGEHCDITVIDPRPEERADAGVSGNGGGRVDNGKGKQKVGDAWGESSSVLMGEEAMV 128
           G + G         P    R+  GV                  D  GES+     E A  
Sbjct: 258 GEKWGAWLRALAARPAQPRRSREGVF---------------QPDEEGESNMPFDREAATE 317

Query: 129 VDSIDDGQTKGVEKALKVNASRGWKRR-ARDAMADVINREASVEVGAGKRKADVASPVPM 188
            D             +     + W      + + +++  E  VE  A     D A PV M
Sbjct: 318 NDP---------SPPVSGGGCKLWDGHWLHELLEEIMQLEMHVERPACSSGKD-APPVTM 377

Query: 189 SVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGCLSVDC 248
             L LN RGLG+ +    L+ L+++  P++VFL ET+++    + ++VRLG  GCL V+ 
Sbjct: 378 RALSLNCRGLGNPQTVNELHNLVKKEGPNIVFLMETRLNVRNLEWLRVRLGMKGCLGVER 437

Query: 249 VGRSGDLALLWSSEIQFSLISFTSNHIDGWVDER-SRQWRFFGIYGHPQAKLKTRTWALM 308
            G+ G LALLW S +  ++ S++ +HIDG V +    +WR  G YG+P+A L+ R+W+L+
Sbjct: 438 HGQGGGLALLWDSSVMINIQSYSEHHIDGEVVQNDGLRWRLTGFYGYPEAHLRHRSWSLL 497

Query: 309 KHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRGEAFT 368
           +HLR  S  PW++ GDFN I    EK G   +    +  F EA+  C L D+GF G  FT
Sbjct: 498 RHLRSISDVPWMIFGDFNEITRLEEKAGREDRNANQMAAFREALLDCSLQDMGFTGTEFT 519

Query: 369 WKPSSDS 374
           W  + ++
Sbjct: 558 WSNNREN 519

BLAST of Lag0027957 vs. ExPASy TrEMBL
Match: A0A2I4F3F9 (uncharacterized protein LOC108995141 OS=Juglans regia OX=51240 GN=LOC108995141 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 2.0e-41
Identity = 115/385 (29.87%), Postives = 187/385 (48.57%), Query Frame = 0

Query: 9   FWCLVLYEQLPNFCYECGCLGHSRRECGSIEGGSLAQGD--PQNIGIIEEAINKVSASRD 68
           FW    YE+LP  C+ CGC+ H    C   +G +    D   +     E    +  A   
Sbjct: 193 FWAPFSYEKLPKICFSCGCMVHGVNGCSGGQGNNEEMEDKWKRESEKKENLETRAGAKEG 252

Query: 69  ASGVQVGEHCDITVIDPRPEERADAG--VSGNGGGRVDNG---KGKQKVGDAWGESSSVL 128
            +G+   E    T    +  E ++ G  +    G R D G   +G+++      E  S  
Sbjct: 253 KNGLGSDEDEGQTRKKEKGREESEGGKDLEEENGDREDEGMMRRGEERWDKQATEEESEN 312

Query: 129 MGEEAM------VVDSIDDGQTK-GVEKALKVNASRGWKRRARDAMADVINREASVEVGA 188
           M ++A+      +V+   +G+   GVE   K      WKRRAR     V++         
Sbjct: 313 MVDKALEETYGQMVEVSSEGKGGIGVEDHPKKKGE--WKRRARAKGKQVVS--------- 372

Query: 189 GKRKADVASPVP---MSVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFD 248
                    P+P   M +L  N RGLG+ R  + LY ++++++P +VF+ ETK     FD
Sbjct: 373 -------DYPIPPNIMKILSWNCRGLGNPRTVQDLYNMVEKNKPSLVFVMETKSLKKSFD 432

Query: 249 SIKVRLGFSGCLSVDCVGRSGDLALLWSSEIQFSLISFTSNHIDGWVDER-SRQWRFFGI 308
           S++ RL   GC  V+ VG+ G L LLW+S+++  +++++ +HI+ W++E   ++W     
Sbjct: 433 SLRRRLQCEGCFVVEAVGKGGGLVLLWNSDVRAEIVNYSQSHINVWIEEEGEKKWLLTCF 492

Query: 309 YGHPQAKLKTRTWALMKHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAM 368
           YG P    +  +W+L+  L+  +   W + GDFN IL + EK GGR +PE  +  F E M
Sbjct: 493 YGQPDTNKRKESWSLLNSLKPAANIGWCIVGDFNEILTNDEKWGGRARPEGQMELFREVM 552

Query: 369 DVCDLMDLGFRGEAFTWKPSSDSKT 376
           +  +L DLG++G+ FTW  S    T
Sbjct: 553 NEGNLYDLGWKGDKFTWSNSHGDDT 559

BLAST of Lag0027957 vs. ExPASy TrEMBL
Match: A0A2N9EV43 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS6587 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 4.6e-41
Identity = 126/431 (29.23%), Postives = 205/431 (47.56%), Query Frame = 0

Query: 8   EFWCLVLYEQLPNFCYECGCLGHSRREC-------GSIEG-----GSLAQGDPQNIGIIE 67
           E W    YE+L NFCY CG + H  ++C       GS+       G+  +G+ + +G   
Sbjct: 188 ECWVSFRYERLTNFCYWCGRITHGEKDCEIWLRSRGSLSSDQQPYGAWMKGESERMGRHP 247

Query: 68  EAINKVSASRDASGVQVG------EHCDITVIDPRPEERADAGVSGNGGGR-VDNGKGKQ 127
             +      R + GVQ G       +C+     P   +  +  VSGN   R  +    +Q
Sbjct: 248 SRLADKLGDR-SGGVQGGMSRRSPANCEEMQQTPPNGKFPNPRVSGNHIPRNQEKDMMEQ 307

Query: 128 KVGDAWGESSSVLM-----GEEAMVVDS-------------------IDDGQTKGVEKAL 187
                 G   +  M     G E +++++                   +D   +  V K  
Sbjct: 308 SKESNLGTQRNACMENRKKGSEEVILENGQEGPNTVTPTVKEVLNGMVDKDPSLFVPKFK 367

Query: 188 KVNASRGWKR---RARDAMADVI----------NREASVEVG----AGKRKADVASPVPM 247
           K   +  WKR   + +D++ DV           N +  VE G     GKR  + A     
Sbjct: 368 KKTPNSTWKRSVGQPKDSLHDVPIFTIAGSKRGNEDIMVEAGEWMKGGKRVCNEA----- 427

Query: 248 SVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGCLSVDC 307
                 V+GLG+ +A R L+ +++   P ++FL ETK++++R + ++V+LGF    SV  
Sbjct: 428 ------VQGLGNPKAVRALHNMVKMKGPKVLFLLETKLNTVRMEGVRVKLGFENVFSVPS 487

Query: 308 VGRSGDLALLWSSEIQFSLISFTSNHIDGWVD-ERSRQWRFFGIYGHPQAKLKTRTWALM 367
           +GRSG LALLW +E +  + +F+ +HID  V+ ++ + WR  G YG P+   +  +WAL+
Sbjct: 488 LGRSGGLALLWQNEAEIVIQNFSQHHIDAHVESQQVKCWRLTGFYGRPEHHRRKESWALL 547

Query: 368 KHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRGEAFT 378
           KHL     APWL  GDFN +L  HEK GG  +    ++ F +A++ C+L+DLG+RG ++T
Sbjct: 548 KHLSTMDSAPWLCLGDFNELLALHEKRGGNARSLRQILEFQDAVNACNLVDLGYRGASYT 606

BLAST of Lag0027957 vs. ExPASy TrEMBL
Match: A0A2N9HN71 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS43499 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 4.6e-41
Identity = 126/431 (29.23%), Postives = 205/431 (47.56%), Query Frame = 0

Query: 8   EFWCLVLYEQLPNFCYECGCLGHSRREC-------GSIEG-----GSLAQGDPQNIGIIE 67
           E W    YE+L NFCY CG + H  ++C       GS+       G+  +G+ + +G   
Sbjct: 154 ECWVSFRYERLTNFCYWCGRITHGEKDCEIWLRSRGSLSSDQQPYGAWMKGESERMGRHP 213

Query: 68  EAINKVSASRDASGVQVG------EHCDITVIDPRPEERADAGVSGNGGGR-VDNGKGKQ 127
             +      R + GVQ G       +C+     P   +  +  VSGN   R  +    +Q
Sbjct: 214 SRLADKLGDR-SGGVQGGMSRRSPANCEEMQQTPPNGKFPNPRVSGNHIPRNQEKDMMEQ 273

Query: 128 KVGDAWGESSSVLM-----GEEAMVVDS-------------------IDDGQTKGVEKAL 187
                 G   +  M     G E +++++                   +D   +  V K  
Sbjct: 274 SKESNLGTQRNACMENRKKGSEEVILENGQEGPNTVTPTVKEVLNGMVDKDPSLFVPKFK 333

Query: 188 KVNASRGWKR---RARDAMADVI----------NREASVEVG----AGKRKADVASPVPM 247
           K   +  WKR   + +D++ DV           N +  VE G     GKR  + A     
Sbjct: 334 KKTPNSTWKRSVGQPKDSLHDVPIFTIAGSKRGNEDIMVEAGEWMKGGKRVCNEA----- 393

Query: 248 SVLLLNVRGLGSARAFRRLYKLIQQHRPHMVFLSETKVHSLRFDSIKVRLGFSGCLSVDC 307
                 V+GLG+ +A R L+ +++   P ++FL ETK++++R + ++V+LGF    SV  
Sbjct: 394 ------VQGLGNPKAVRALHNMVKMKGPKVLFLLETKLNTVRMEGVRVKLGFENVFSVPS 453

Query: 308 VGRSGDLALLWSSEIQFSLISFTSNHIDGWVD-ERSRQWRFFGIYGHPQAKLKTRTWALM 367
           +GRSG LALLW +E +  + +F+ +HID  V+ ++ + WR  G YG P+   +  +WAL+
Sbjct: 454 LGRSGGLALLWQNEAEIVIQNFSQHHIDAHVESQQVKCWRLTGFYGRPEHHRRKESWALL 513

Query: 368 KHLRGRSVAPWLVGGDFNVILFHHEKDGGRLKPEAGLVGFHEAMDVCDLMDLGFRGEAFT 378
           KHL     APWL  GDFN +L  HEK GG  +    ++ F +A++ C+L+DLG+RG ++T
Sbjct: 514 KHLSTMDSAPWLCLGDFNELLALHEKRGGNARSLRQILEFQDAVNACNLVDLGYRGASYT 572

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_018826186.14.2e-4129.87uncharacterized protein LOC108995141 [Juglans regia] >KAF5471486.1 hypothetical ... [more]
KAF5449841.11.2e-4047.80hypothetical protein F2P56_030246 [Juglans regia][more]
XP_030970584.12.3e-3945.90uncharacterized protein LOC115990959 [Quercus lobata][more]
XP_022841874.12.6e-3828.47uncharacterized protein LOC111365549 [Olea europaea var. sylvestris][more]
OMO59710.13.4e-3842.08reverse transcriptase [Corchorus capsularis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2N9EWI98.3e-4331.06Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9GJ352.4e-4231.88Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS27322 PE=3 SV=1[more]
A0A2I4F3F92.0e-4129.87uncharacterized protein LOC108995141 OS=Juglans regia OX=51240 GN=LOC108995141 P... [more]
A0A2N9EV434.6e-4129.23Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9HN714.6e-4129.23Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025836Zinc knuckle CX2CX4HX4CPFAMPF14392zf-CCHC_4coord: 7..35
e-value: 5.5E-9
score: 35.6
IPR036691Endonuclease/exonuclease/phosphatase superfamilyGENE3D3.60.10.10Endonuclease/exonuclease/phosphatasecoord: 185..378
e-value: 4.6E-20
score: 74.4
IPR036691Endonuclease/exonuclease/phosphatase superfamilySUPERFAMILY56219DNase I-likecoord: 184..368
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..112
NoneNo IPR availablePANTHERPTHR22748:SF11DNA-(APURINIC OR APYRIMIDINIC SITE) LYASE CHLOROPLASTICcoord: 154..338
IPR004808AP endonuclease 1PANTHERPTHR22748AP ENDONUCLEASEcoord: 154..338
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 22..35
score: 9.1912

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027957.1Lag0027957.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006281 DNA repair
molecular_function GO:0004518 nuclease activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding