Lag0027486 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027486
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionmitochondrial import receptor subunit TOM20-like
Locationchr8: 1339490 .. 1340919 (-)
RNA-Seq ExpressionLag0027486
SyntenyLag0027486
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTTGCAGGCCAGCGAGTTCGATCGCATACTTTTCTTCGAGCACGCTCGCAAAAATGCCGAAGCCACTTACGCCAGAAATCCCCTCGATGTCGATGTGAGTTTTCCCCCTTTTTTCGCCCTCTTAATCTACCCATCGGGTTATTTGTGTTTATTTTGTTGTTACTTTTGTTGTTATTTTTTCTGGGTTCTTGGAGGATTGTGCGATGTTCGATTCTGTGCACAAGGGCTTGGTTAAATTGTGTGAAATGTTATGAACTATAGGGTTGGTTTCAATTGTGTTTGAATTTTCGTTTTAACTGGCTTGTTAGTTGTCTAAAAAAACCCTTTGGTTGCCAGCATTCATAGATAGTAGCACAATTATGGCCTCACTTTTTGTTTGTTGGTAGAGGGAGGGATATAAAGAAAAATGATAGTTCCCTGTATCTTTTTCCCCTCTCAAACCTTAATATCCGAGCTTCCATTAGGTTAGCAGCTAAATTTAATGGGGAACTAACGAAACTTAAAAGTTTTGGATAATAAGGAGATGTTTAAAACCATTGGGGCATAGTTGAAACTAATCCGATAGTTCATTATATAATGTGCCCCTCTTTCTATATTGGGTGTAATATTTATGACTCTGGAGCTGTATTTGATAATCAAACGTGGATGGACTTTCATGTAGAATTTGACAAGATGGGCAGGAGCTTTGTTAGAGCTCTCTCAGTTTCAGAGCGTTCCGGAATCAAAGAAGATGATTCTAGGTATCTTTTTGTTCTACAAATTCAGATGATGGAGCATGGGAATTGAAAATATTTTTCTTGGCTTTGATTTGTGGCTGACTTGGAGATTTTTATAATTCATTTTTCTTTTTCTACATATGCATTAATGCCAGCTCTTTCCTATAATTTAATGATTTTAGTGATGGGCATTGAGTAATGAGTATATTGTGGATTAATTTGTGATGGGGTTGAGACAGATTCAATATCAAAGCTCGAGGAGGCACTGTTGATTAACCCCAAGAAGCATGATGCTCTTTGGTGCTTAGGAAATGCTTATACTTCCCATGCATTTTTGAACCCAGACCAGGATGAGGCAAAAGATTATTTTGATAAGGCCTCAATATGCTTTCAGCAAGCTGTGGATGAGGTACTAAGTCCGGAAAACCTCACAATGAGCTTATAAACATGCGTTTCCTGATGGCTTTTCTAGTTTAACACGGTGATTTGTTGTATTCAGGATCCTGGGAATGAACTTTACCTGAAGTCCTTGGAGGTGACTGCTAAGGTATTTCTTTGTAATTTTGATTTCAACATTCTCTGGTGCAATTTGAATATATGTCTATTTTCAAGAATATTACTAAGCCCCTTTCAATGGCTTTGAAATCAGAGAATCTTTTTGTTTCATTTGTGCGTTGATGAAAAGTTAACTGCTGATCTATGTTTGTAA

mRNA sequence

ATGGATTTGCAGGCCAGCGAGTTCGATCGCATACTTTTCTTCGAGCACGCTCGCAAAAATGCCGAAGCCACTTACGCCAGAAATCCCCTCGATGTCGATAATTTGACAAGATGGGCAGGAGCTTTGTTAGAGCTCTCTCAGTTTCAGAGCGTTCCGGAATCAAAGAAGATGATTCTAGATTCAATATCAAAGCTCGAGGAGGCACTGTTGATTAACCCCAAGAAGCATGATGCTCTTTGGTGCTTAGGAAATGCTTATACTTCCCATGCATTTTTGAACCCAGACCAGGATGAGGCAAAAGATTATTTTGATAAGGCCTCAATATGCTTTCAGCAAGCTGTGGATGAGGATCCTGGGAATGAACTTTACCTGAAGTCCTTGGAGGTGACTGCTAAGAGAATCTTTTTGTTTCATTTGTGCGTTGATGAAAAGTTAACTGCTGATCTATGTTTGTAA

Coding sequence (CDS)

ATGGATTTGCAGGCCAGCGAGTTCGATCGCATACTTTTCTTCGAGCACGCTCGCAAAAATGCCGAAGCCACTTACGCCAGAAATCCCCTCGATGTCGATAATTTGACAAGATGGGCAGGAGCTTTGTTAGAGCTCTCTCAGTTTCAGAGCGTTCCGGAATCAAAGAAGATGATTCTAGATTCAATATCAAAGCTCGAGGAGGCACTGTTGATTAACCCCAAGAAGCATGATGCTCTTTGGTGCTTAGGAAATGCTTATACTTCCCATGCATTTTTGAACCCAGACCAGGATGAGGCAAAAGATTATTTTGATAAGGCCTCAATATGCTTTCAGCAAGCTGTGGATGAGGATCCTGGGAATGAACTTTACCTGAAGTCCTTGGAGGTGACTGCTAAGAGAATCTTTTTGTTTCATTTGTGCGTTGATGAAAAGTTAACTGCTGATCTATGTTTGTAA

Protein sequence

MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILDSISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGNELYLKSLEVTAKRIFLFHLCVDEKLTADLCL
Homology
[Close]
BLAST of Lag0027486 vs. NCBI nr
Match: XP_022984139.1 (mitochondrial import receptor subunit TOM20-like [Cucurbita maxima])

HSP 1 Score: 252.7 bits (644), Expect = 2.0e-63
Identity = 121/132 (91.67%), Postives = 128/132 (96.97%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           MDLQ +EFDR+LFFEHARKNAEA+YA NPLD DNLTRWAGALLELSQFQSVPESKKMILD
Sbjct: 1   MDLQPNEFDRLLFFEHARKNAEASYANNPLDADNLTRWAGALLELSQFQSVPESKKMILD 60

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           SISKLEEALLINPKKHDALWC+GNAYTSHAFLNP+QDEAKDYFDKAS+ FQQAV+EDPGN
Sbjct: 61  SISKLEEALLINPKKHDALWCIGNAYTSHAFLNPNQDEAKDYFDKASVYFQQAVNEDPGN 120

Query: 121 ELYLKSLEVTAK 133
           ELYLKSLEVTAK
Sbjct: 121 ELYLKSLEVTAK 132

[Close]
BLAST of Lag0027486 vs. NCBI nr
Match: KAG6581098.1 (Mitochondrial import receptor subunit TOM20, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 252.3 bits (643), Expect = 2.7e-63
Identity = 121/132 (91.67%), Postives = 127/132 (96.21%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           MDLQ +EFDR+LFFEHARKNAEA YA NPLD DNLTRWAGALLELSQFQSVPESKKMILD
Sbjct: 57  MDLQPNEFDRLLFFEHARKNAEAAYANNPLDADNLTRWAGALLELSQFQSVPESKKMILD 116

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           SISKLEEALLINPKKHDALWC+GNAYTSHAFLNP+QDEAKDYFDKAS+ FQQAV+EDPGN
Sbjct: 117 SISKLEEALLINPKKHDALWCIGNAYTSHAFLNPNQDEAKDYFDKASVYFQQAVNEDPGN 176

Query: 121 ELYLKSLEVTAK 133
           ELYLKSLEVTAK
Sbjct: 177 ELYLKSLEVTAK 188

[Close]
BLAST of Lag0027486 vs. NCBI nr
Match: XP_022935684.1 (mitochondrial import receptor subunit TOM20-like [Cucurbita moschata] >XP_023526202.1 mitochondrial import receptor subunit TOM20-like [Cucurbita pepo subsp. pepo] >KAG7017828.1 Mitochondrial import receptor subunit TOM20, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 252.3 bits (643), Expect = 2.7e-63
Identity = 121/132 (91.67%), Postives = 127/132 (96.21%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           MDLQ +EFDR+LFFEHARKNAEA YA NPLD DNLTRWAGALLELSQFQSVPESKKMILD
Sbjct: 1   MDLQPNEFDRLLFFEHARKNAEAAYANNPLDADNLTRWAGALLELSQFQSVPESKKMILD 60

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           SISKLEEALLINPKKHDALWC+GNAYTSHAFLNP+QDEAKDYFDKAS+ FQQAV+EDPGN
Sbjct: 61  SISKLEEALLINPKKHDALWCIGNAYTSHAFLNPNQDEAKDYFDKASVYFQQAVNEDPGN 120

Query: 121 ELYLKSLEVTAK 133
           ELYLKSLEVTAK
Sbjct: 121 ELYLKSLEVTAK 132

[Close]
BLAST of Lag0027486 vs. NCBI nr
Match: XP_004145663.1 (mitochondrial import receptor subunit TOM20 [Cucumis sativus] >KGN58173.1 hypothetical protein Csa_017635 [Cucumis sativus])

HSP 1 Score: 249.2 bits (635), Expect = 2.2e-62
Identity = 119/132 (90.15%), Postives = 129/132 (97.73%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           MDLQASEFDRILFFEHARKNAE+TYA NPLD +NLTRWAGALLELSQFQSVPESKKMILD
Sbjct: 1   MDLQASEFDRILFFEHARKNAESTYATNPLDAENLTRWAGALLELSQFQSVPESKKMILD 60

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           SISKLEEAL+INPKKHDALWCLGNAYTSHAFLNP+QDEAK++FDKA+I F+QAV+EDPGN
Sbjct: 61  SISKLEEALMINPKKHDALWCLGNAYTSHAFLNPNQDEAKEFFDKATIYFKQAVEEDPGN 120

Query: 121 ELYLKSLEVTAK 133
           E+YLKSLEVTAK
Sbjct: 121 EIYLKSLEVTAK 132

[Close]
BLAST of Lag0027486 vs. NCBI nr
Match: XP_022989712.1 (mitochondrial import receptor subunit TOM20-like [Cucurbita maxima])

HSP 1 Score: 248.8 bits (634), Expect = 2.9e-62
Identity = 120/132 (90.91%), Postives = 127/132 (96.21%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           MDLQ S+FDR+LFFEHARKNAEATYA+NPLD DNLTRWAGALLELSQFQSV ESKKMILD
Sbjct: 1   MDLQPSDFDRLLFFEHARKNAEATYAKNPLDADNLTRWAGALLELSQFQSVIESKKMILD 60

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           SI+KLEEALLINPKKHDALWCLGNAYTSHAFLNP+QDEAK YFDKAS+ F+QAVDEDPGN
Sbjct: 61  SIAKLEEALLINPKKHDALWCLGNAYTSHAFLNPNQDEAKSYFDKASVYFKQAVDEDPGN 120

Query: 121 ELYLKSLEVTAK 133
           ELYLKSLEVTAK
Sbjct: 121 ELYLKSLEVTAK 132

[Close]
BLAST of Lag0027486 vs. ExPASy Swiss-Prot
Match: P92792 (Mitochondrial import receptor subunit TOM20 OS=Solanum tuberosum OX=4113 GN=TOM20 PE=1 SV=1)

HSP 1 Score: 211.1 bits (536), Expect = 8.9e-54
Identity = 101/132 (76.52%), Postives = 114/132 (86.36%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           M++Q SEFDR+LFFEHARK+AE TYA+NPLD DNLTRW GALLELSQFQ V ESK+MI D
Sbjct: 1   MEMQ-SEFDRLLFFEHARKSAETTYAQNPLDADNLTRWGGALLELSQFQPVAESKQMISD 60

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           + SKLEEAL +NP+KHDALWCLGNA+TSH FL PD DEAK YF+KA+ CFQQA D DP N
Sbjct: 61  ATSKLEEALTVNPEKHDALWCLGNAHTSHVFLTPDMDEAKVYFEKATQCFQQAFDADPSN 120

Query: 121 ELYLKSLEVTAK 133
           +LY KSLEVTAK
Sbjct: 121 DLYRKSLEVTAK 131

[Close]
BLAST of Lag0027486 vs. ExPASy Swiss-Prot
Match: P82874 (Mitochondrial import receptor subunit TOM20-3 OS=Arabidopsis thaliana OX=3702 GN=TOM20-3 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 7.1e-43
Identity = 84/127 (66.14%), Postives = 101/127 (79.53%), Query Frame = 0 

Query: 6   SEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILDSISKL 65
           +EFDRIL FE  R++AE TY  NPLD DNLTRW G LLELSQF S+ ++K+MI ++I+K 
Sbjct: 5   TEFDRILLFEQIRQDAENTYKSNPLDADNLTRWGGVLLELSQFHSISDAKQMIQEAITKF 64

Query: 66  EEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGNELYLK 125
           EEALLI+PKK +A+WC+GNAYTS AFL PD+ EAK  FD A+  FQQAVDE P N  YLK
Sbjct: 65  EEALLIDPKKDEAVWCIGNAYTSFAFLTPDETEAKHNFDLATQFFQQAVDEQPDNTHYLK 124

Query: 126 SLEVTAK 133
           SLE+TAK
Sbjct: 125 SLEMTAK 131

[Close]
BLAST of Lag0027486 vs. ExPASy Swiss-Prot
Match: P82873 (Mitochondrial import receptor subunit TOM20-2 OS=Arabidopsis thaliana OX=3702 GN=TOM20-2 PE=1 SV=2)

HSP 1 Score: 174.1 bits (440), Expect = 1.2e-42
Identity = 79/132 (59.85%), Postives = 104/132 (78.79%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           M+   ++F+R++ FEHARKN+EA Y  +PLD +NL +W GALLELSQFQ +PE+K M+ D
Sbjct: 1   MEFSTADFERLIMFEHARKNSEAQYKNDPLDSENLLKWGGALLELSQFQPIPEAKLMLND 60

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           +ISKLEEAL INP KH ALWC+ NAYT+HAF   D +EAK++FDKA+  FQ+A +EDPGN
Sbjct: 61  AISKLEEALTINPGKHQALWCIANAYTAHAFYVHDPEEAKEHFDKATEYFQRAENEDPGN 120

Query: 121 ELYLKSLEVTAK 133
           + Y KSL+ + K
Sbjct: 121 DTYRKSLDSSLK 132

[Close]
BLAST of Lag0027486 vs. ExPASy Swiss-Prot
Match: P82805 (Mitochondrial import receptor subunit TOM20-4 OS=Arabidopsis thaliana OX=3702 GN=TOM20-4 PE=1 SV=1)

HSP 1 Score: 164.1 bits (414), Expect = 1.2e-39
Identity = 84/132 (63.64%), Postives = 105/132 (79.55%), Query Frame = 0 

Query: 1   MDLQASEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMILD 60
           MD+Q +E +R++ FEHARK AEATY +NPLD +NLTRWAGALLELSQFQ+ P  K+MIL+
Sbjct: 1   MDMQ-NENERLMVFEHARKVAEATYVKNPLDAENLTRWAGALLELSQFQTEP--KQMILE 60

Query: 61  SISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPGN 120
           +I KL EAL+I+PKKHDALW +GNA+ S  FL+ DQ EA D F+KAS  FQ AV+E P +
Sbjct: 61  AILKLGEALVIDPKKHDALWLIGNAHLSFGFLSSDQTEASDNFEKASQFFQLAVEEQPES 120

Query: 121 ELYLKSLEVTAK 133
           ELY KSL + +K
Sbjct: 121 ELYRKSLTLASK 129

[Close]
BLAST of Lag0027486 vs. ExPASy Swiss-Prot
Match: A2WYG9 (Probable mitochondrial import receptor subunit TOM20 OS=Oryza sativa subsp. indica OX=39946 GN=TOM20 PE=2 SV=2)

HSP 1 Score: 154.8 bits (390), Expect = 7.6e-37
Identity = 76/133 (57.14%), Postives = 101/133 (75.94%), Query Frame = 0 

Query: 1   MDLQA-SEFDRILFFEHARKNAEATYARNPLDVDNLTRWAGALLELSQFQSVPESKKMIL 60
           MD+ A S+ +R+ FF+ A +NA+ TY +NP D DNLTRW GALLELSQ ++ PES K + 
Sbjct: 1   MDMGAMSDPERMFFFDLACQNAKVTYEQNPHDADNLTRWGGALLELSQMRNGPESLKCLE 60

Query: 61  DSISKLEEALLINPKKHDALWCLGNAYTSHAFLNPDQDEAKDYFDKASICFQQAVDEDPG 120
           D+ SKLEEAL I+P K DALWCLGNA TSH F   D  +A ++F+KA+ CFQ+AVD +P 
Sbjct: 61  DAESKLEEALKIDPMKADALWCLGNAQTSHGFFTSDTVKANEFFEKATQCFQKAVDVEPA 120

Query: 121 NELYLKSLEVTAK 133
           N+LY KSL++++K
Sbjct: 121 NDLYRKSLDLSSK 133

The following BLAST results are available for this feature:
BLAST of Lag0027486 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022984139.12.0e-6391.67mitochondrial import receptor subunit TOM20-like [Cucurbita maxima][more]
KAG6581098.12.7e-6391.67Mitochondrial import receptor subunit TOM20, partial [Cucurbita argyrosperma sub... [more]
XP_022935684.12.7e-6391.67mitochondrial import receptor subunit TOM20-like [Cucurbita moschata] >XP_023526... [more]
XP_004145663.12.2e-6290.15mitochondrial import receptor subunit TOM20 [Cucumis sativus] >KGN58173.1 hypoth... [more]
XP_022989712.12.9e-6290.91mitochondrial import receptor subunit TOM20-like [Cucurbita maxima][more]
BLAST of Lag0027486 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
P927928.9e-5476.52Mitochondrial import receptor subunit TOM20 OS=Solanum tuberosum OX=4113 GN=TOM2... [more]
P828747.1e-4366.14Mitochondrial import receptor subunit TOM20-3 OS=Arabidopsis thaliana OX=3702 GN... [more]
P828731.2e-4259.85Mitochondrial import receptor subunit TOM20-2 OS=Arabidopsis thaliana OX=3702 GN... [more]
P828051.2e-3963.64Mitochondrial import receptor subunit TOM20-4 OS=Arabidopsis thaliana OX=3702 GN... [more]
A2WYG97.6e-3757.14Probable mitochondrial import receptor subunit TOM20 OS=Oryza sativa subsp. indi... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF06552TOM20_plantcoord: 8..134
e-value: 4.4E-68
score: 228.7
NoneNo IPR availablePANTHERPTHR32409:SF3MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATEDcoord: 1..133
IPR011990Tetratricopeptide-like helical domain superfamilyGENE3D1.25.40.10Tetratricopeptide repeat domaincoord: 1..147
e-value: 8.1E-65
score: 219.1
IPR011990Tetratricopeptide-like helical domain superfamilySUPERFAMILY48452TPR-likecoord: 13..127
IPR010547Plant specific mitochondrial import receptor subunit TOM20PANTHERPTHR32409MITOCHONDRIAL IMPORT RECEPTOR SUBUNIT TOM20-1-RELATEDcoord: 1..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027486.1Lag0027486.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0045040 protein insertion into mitochondrial outer membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005742 mitochondrial outer membrane translocase complex
molecular_function GO:0005515 protein binding