Lag0027288 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027288
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptiondnaJ protein ERDJ3A
Locationchr10: 46500232 .. 46503658 (+)
RNA-Seq ExpressionLag0027288
SyntenyLag0027288
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGACTCGCCTAGCGCCGATCGTCCTCATTATGGCGTCGCTGTTCCTCTTCCTCTTGTCTTTAGAAGCCAAAACCATGGACCCCTATAAGGTAACGATGATTGTTCTTGGATTTCTCTATGTTTCTCGACCTGTTTTCTTTTGATATTCGGCATGATTTTCGCCTAATTGTTTGGTACTTGAGAATTTTACATTCGTTTTGTTTATGGAGTATCGAAGTTTTGTGGCGGTGTTATAGAATGGCTCATTTTTGTTGCATTCTTCGAAATTGTTTTCAGGTTCTTGGAGTTGAGCGGAATGCGAGTCAGCGGGAAATTCAGAAAGCTTTCCACAAGTATGTTCGTTGATTTCCCTCCAAAATCTTTGTTCTTCTTCAATTTGATTCGACTTCACGTGGTTGTGTAGCGCATGACATTATTATTATTATTATTATTATTATTTTTGTGCGTTCTTGTTTACTGAATCTCATCTTTGTCTTCTTAAGTACATTGTGGGACTGATATTGTGGTGGATTTTGTTGATTTTGCTATCCTATATTTGTTTTGTTACAATTTCTTCTTAAAGCTTAATTTCTGGGTTAGTCATTCTCACTTGTTCGGGTTTTTATGCATCCTTGGCAGGCTCTCTCTTCAGTATCACCCAGATAAGAATAAGAAAAAAGGCGCTCAACAGAAGTTTGAAGAAATCAACAACGGTATAATACTGCAGTTCATCTCTTTTCACTCTATATCTTTTTGAATTTTTGGATGATTTTTGGTCGTCAGGGGGGTTTATTTAGTTTGACTCACGAAGTTCAACATTGGAGAGAAAACAGCTTTCTCTTGCTTTGATAGCATGTTAAACTGCTATTAAACCAAAATGTCTAAATATTCACTTTTCTCTTATGTACTCAAAATATGACTAGTTTTCCACCACCATTGACCTTCCTCTTTCATTCCTCAGCTTATGAAATTTTATCAGATGAAGAGAAGAGAAAAAATTTTGACATGTTTGGCGATGAAAAAGGTAGCCCTGGATTTGGCCCTGGCTTTTCAGGTGATCAAGGTGGCGGATATACATTCTTTACAAACGAAGGAAGAAGACAGGGTCAGCAGACCTTCAGGCCAGGGTGGCAGAGTACTGGTGGTCAGGGAGGTTCACAGTCTTTCTCATTTTCCTTTGGTGGGCCTACTGGTTCAAATCCTTTCAGCAGCTTCGGTATGGAAGATATATTCTCCAACCTTTTTGGAGGTGGCTTTAAGGATGAGGGTAGTTTTGGTAGTTTTGGTGGTTCTAAGGCTCAATCTGGATCTAAGAGTTCCTCAAGCATCAGGGTCATTAATACACAAGTATTCAAGAAAGAAATTGTGGATCAGGGAATGACTTGGCTTTTGTTTCCAAGCACACCATCTTTAAAGGGGTTTGATCATGTTCAATCAATCATGGAGGAAGTGGCCAGTTCATTGCTGGGAGCTCTAAAGGTGCTAGATTAAATAACTATATTTCTAAATTAAATAGTTCTGGTGATTTTTCACTGTTCTTATGCGCATTTTTATGTTTTGATTTGAAGGTTGGAAGGATAGATTGTGAATCAGAATCCTTTCTCTGCAAGAACCTCGGCATTTATCCTCACAGGAATCCCAAAATATTTGTATATTCATACATTAAAAGCACCGAGGGTTCTGAGGGTTCTTTGGTGGAGTACAATGGGGACTTTACCGTGAAGAACTTGAAGAGCTTTTGCCAAGAGCATTTGCCTAGGTTCTCACAACGAGTCAATTTGAATCAATTCGATTTCTCTTCCAGCAATGGACGGGGACTACCCACCTTAATGCTTTTGTCAACCAAAAAAGATACACCTGTTATCTGGCGTGTCCTTAGCGGGCTGTATCGCAAGCAGTTCAGTTTCTACGATGCAGAGGTATTTTATAACTAAGCCTGCTTGATTATCTCATTCTTGTTTGAGGCTAGTATCTTTTAATCGGTGTCTAAGTTGGGGAGTATTTCTATCAGTGTATGAATTATATTAATAGCAGTATCCATATCATAATTTTTTTGGGTTATTTCTATGGAGGTTCATCATTTTCGTACCCTGATTAGGTAATTTTTTTTCTATTACATAGGTCACTGATGTTTCAGATCCTACAATAAAGAAGTTAGGAGTTGACGCCCTTCCAGCTATAGTGGGTTGGCTATCAAATGGGGAGAGGCACGTTCTTAGAACAGGGATCAATGTGAAAGATTTGAGATCTGCCATCGATGATCTCAGTAATTTACTAAATGATTTTGAGAAAAAGAATAGGAAGGAAGCATCAAGGCCAGCCTCCAAAACACCATCTGACTCAACAGAGAACCAGATCCCACTATTGACAAAATCCAACTTTGACGCTGTCTGTGGGGAGAAGACTCCTGTTTGTGTTATTGGTGCCTTCAGATCTTCTAAAGCAAGGAACAAATTGGAATTAATCCTAAATACGGTGAGAACACTTACACTCACGTAAATGTACTTCCTCAGTTGAATCTGATTGAAATAGAACACATGTTATTGTCATTTTATTCATGTTTTGGATAAATAATTGGAAAGGCTGTGAACGGTAACTGATTATGAAAACTCTATCAACCTCAGGTCTTGATGAACTGTTCAAGTAGACTACAGATGATTGGGATGCTACTAGGGTAGGATTAGGAATGTATTTGGGCATATTAGTAATTAGCTAGGAAAGTTGAGAGTGAGTGGAATCGGATGGGATAAGGAATTGTGTAGATGATTTAAGCTCGAGTTCAGTAAACACGATCGAATTGTTTGGGTTAACTTGTAGTTATTTACCTTTTCTTATTACTATTCTAACTATTCTAGGTTGGGTTCTATCAACAAACATGGTCGATTTATAATTATTGTTGCCTGAAGTGTATTGCACGTAATTCTCGAAGGAATTAACTACATTTGTCTTAATATTTGTTGCTGGAGTGAATTTGATTCTTCTTTTTGGAACAGTAAAAAACGCATGTAACTGATTAAATGCATTTCTTTTTCAAAAACTTTCATAGTTGCTGCAGAGCCAAAGTCATTACAAAGTACTAATCTATCTCAAGCTTCTGCATTTTTTACTTGTTTGATTGTTGCTATTTCATATCTTACATTTTCTTTCCCTCTTGATTTTATCTTTCAGGTATCTCAGAAGACATTGTCAAGGACGCCTGGCTCTAAGGACTCCATATCCTATGCTCTTATAGATGCAACAAAGCAACCATCATTTCTGAATGCATTTGACAGTGCAGGATATAAATCATCTGATAAAATATTGATCGCATACAAACGTCGAAAGGGCAAATTTGCTGCACATTTGGGCGAAATGACAGAAGAAGAAGTAGAAAGATTCATTGGCTCGGTACTTAATGGGGACGTTATATTTTCCAAGACCCGCCAGAATCCTGTCTTGAAGTGA

mRNA sequence

ATGGAGACTCGCCTAGCGCCGATCGTCCTCATTATGGCGTCGCTGTTCCTCTTCCTCTTGTCTTTAGAAGCCAAAACCATGGACCCCTATAAGGTTCTTGGAGTTGAGCGGAATGCGAGTCAGCGGGAAATTCAGAAAGCTTTCCACAAGCTCTCTCTTCAGTATCACCCAGATAAGAATAAGAAAAAAGGCGCTCAACAGAAGTTTGAAGAAATCAACAACGCTTATGAAATTTTATCAGATGAAGAGAAGAGAAAAAATTTTGACATGTTTGGCGATGAAAAAGGTAGCCCTGGATTTGGCCCTGGCTTTTCAGGTGATCAAGGTGGCGGATATACATTCTTTACAAACGAAGGAAGAAGACAGGGTCAGCAGACCTTCAGGCCAGGGTGGCAGAGTACTGGTGGTCAGGGAGGTTCACAGTCTTTCTCATTTTCCTTTGGTGGGCCTACTGGTTCAAATCCTTTCAGCAGCTTCGGTATGGAAGATATATTCTCCAACCTTTTTGGAGGTGGCTTTAAGGATGAGGGTAGTTTTGGTAGTTTTGGTGGTTCTAAGGCTCAATCTGGATCTAAGAGTTCCTCAAGCATCAGGGTCATTAATACACAAGTATTCAAGAAAGAAATTGTGGATCAGGGAATGACTTGGCTTTTGTTTCCAAGCACACCATCTTTAAAGGGGTTTGATCATGTTCAATCAATCATGGAGGAAGTGGCCAGTTCATTGCTGGGAGCTCTAAAGGTTGGAAGGATAGATTGTGAATCAGAATCCTTTCTCTGCAAGAACCTCGGCATTTATCCTCACAGGAATCCCAAAATATTTGTATATTCATACATTAAAAGCACCGAGGGTTCTGAGGGTTCTTTGGTGGAGTACAATGGGGACTTTACCGTGAAGAACTTGAAGAGCTTTTGCCAAGAGCATTTGCCTAGGTTCTCACAACGAGTCAATTTGAATCAATTCGATTTCTCTTCCAGCAATGGACGGGGACTACCCACCTTAATGCTTTTGTCAACCAAAAAAGATACACCTGTTATCTGGCGTGTCCTTAGCGGGCTGTATCGCAAGCAGTTCAGTTTCTACGATGCAGAGGTCACTGATGTTTCAGATCCTACAATAAAGAAGTTAGGAGTTGACGCCCTTCCAGCTATAGTGGGTTGGCTATCAAATGGGGAGAGGCACGTTCTTAGAACAGGGATCAATGTGAAAGATTTGAGATCTGCCATCGATGATCTCAGTAATTTACTAAATGATTTTGAGAAAAAGAATAGGAAGGAAGCATCAAGGCCAGCCTCCAAAACACCATCTGACTCAACAGAGAACCAGATCCCACTATTGACAAAATCCAACTTTGACGCTGTCTGTGGGGAGAAGACTCCTGTTTGTGTTATTGGTGCCTTCAGATCTTCTAAAGCAAGGAACAAATTGGAATTAATCCTAAATACGGTATCTCAGAAGACATTGTCAAGGACGCCTGGCTCTAAGGACTCCATATCCTATGCTCTTATAGATGCAACAAAGCAACCATCATTTCTGAATGCATTTGACAGTGCAGGATATAAATCATCTGATAAAATATTGATCGCATACAAACGTCGAAAGGGCAAATTTGCTGCACATTTGGGCGAAATGACAGAAGAAGAAGTAGAAAGATTCATTGGCTCGGTACTTAATGGGGACGTTATATTTTCCAAGACCCGCCAGAATCCTGTCTTGAAGTGA

Coding sequence (CDS)

ATGGAGACTCGCCTAGCGCCGATCGTCCTCATTATGGCGTCGCTGTTCCTCTTCCTCTTGTCTTTAGAAGCCAAAACCATGGACCCCTATAAGGTTCTTGGAGTTGAGCGGAATGCGAGTCAGCGGGAAATTCAGAAAGCTTTCCACAAGCTCTCTCTTCAGTATCACCCAGATAAGAATAAGAAAAAAGGCGCTCAACAGAAGTTTGAAGAAATCAACAACGCTTATGAAATTTTATCAGATGAAGAGAAGAGAAAAAATTTTGACATGTTTGGCGATGAAAAAGGTAGCCCTGGATTTGGCCCTGGCTTTTCAGGTGATCAAGGTGGCGGATATACATTCTTTACAAACGAAGGAAGAAGACAGGGTCAGCAGACCTTCAGGCCAGGGTGGCAGAGTACTGGTGGTCAGGGAGGTTCACAGTCTTTCTCATTTTCCTTTGGTGGGCCTACTGGTTCAAATCCTTTCAGCAGCTTCGGTATGGAAGATATATTCTCCAACCTTTTTGGAGGTGGCTTTAAGGATGAGGGTAGTTTTGGTAGTTTTGGTGGTTCTAAGGCTCAATCTGGATCTAAGAGTTCCTCAAGCATCAGGGTCATTAATACACAAGTATTCAAGAAAGAAATTGTGGATCAGGGAATGACTTGGCTTTTGTTTCCAAGCACACCATCTTTAAAGGGGTTTGATCATGTTCAATCAATCATGGAGGAAGTGGCCAGTTCATTGCTGGGAGCTCTAAAGGTTGGAAGGATAGATTGTGAATCAGAATCCTTTCTCTGCAAGAACCTCGGCATTTATCCTCACAGGAATCCCAAAATATTTGTATATTCATACATTAAAAGCACCGAGGGTTCTGAGGGTTCTTTGGTGGAGTACAATGGGGACTTTACCGTGAAGAACTTGAAGAGCTTTTGCCAAGAGCATTTGCCTAGGTTCTCACAACGAGTCAATTTGAATCAATTCGATTTCTCTTCCAGCAATGGACGGGGACTACCCACCTTAATGCTTTTGTCAACCAAAAAAGATACACCTGTTATCTGGCGTGTCCTTAGCGGGCTGTATCGCAAGCAGTTCAGTTTCTACGATGCAGAGGTCACTGATGTTTCAGATCCTACAATAAAGAAGTTAGGAGTTGACGCCCTTCCAGCTATAGTGGGTTGGCTATCAAATGGGGAGAGGCACGTTCTTAGAACAGGGATCAATGTGAAAGATTTGAGATCTGCCATCGATGATCTCAGTAATTTACTAAATGATTTTGAGAAAAAGAATAGGAAGGAAGCATCAAGGCCAGCCTCCAAAACACCATCTGACTCAACAGAGAACCAGATCCCACTATTGACAAAATCCAACTTTGACGCTGTCTGTGGGGAGAAGACTCCTGTTTGTGTTATTGGTGCCTTCAGATCTTCTAAAGCAAGGAACAAATTGGAATTAATCCTAAATACGGTATCTCAGAAGACATTGTCAAGGACGCCTGGCTCTAAGGACTCCATATCCTATGCTCTTATAGATGCAACAAAGCAACCATCATTTCTGAATGCATTTGACAGTGCAGGATATAAATCATCTGATAAAATATTGATCGCATACAAACGTCGAAAGGGCAAATTTGCTGCACATTTGGGCGAAATGACAGAAGAAGAAGTAGAAAGATTCATTGGCTCGGTACTTAATGGGGACGTTATATTTTCCAAGACCCGCCAGAATCCTGTCTTGAAGTGA

Protein sequence

METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGRRQGQQTFRPGWQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSFGSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVASSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVKNLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFSFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDFEKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELILNTVSQKTLSRTPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKGKFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK
Homology
BLAST of Lag0027288 vs. NCBI nr
Match: XP_038905464.1 (dnaJ protein ERDJ3A isoform X1 [Benincasa hispida])

HSP 1 Score: 959.9 bits (2480), Expect = 9.8e-276
Identity = 502/579 (86.70%), Postives = 531/579 (91.71%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           ME RLA I LIMA LFLFL SLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLASITLIMAFLFLFLCSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKKGAQQKFEEINNAYEILSDEEKRKNFDM+G+EKGSPGFGPG S DQGGGYTFFTN   
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGNEKGSPGFGPGSSEDQGGGYTFFTNGAG 120

Query: 121 RQGQQTFRPG-WQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F PG WQSTGGQGGSQSFSFSFGGP+GSNPF  FGMEDIFSN FGGGFKDEG+F
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPF-GFGMEDIFSNFFGGGFKDEGNF 180

Query: 181 GSFGG-SKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEV 240
           GSFG  SKAQSGS++SSSI+VIN QVFKKEIVDQGMTWLLFP+T SLKG DHVQSI+EEV
Sbjct: 181 GSFGSHSKAQSGSRTSSSIKVINKQVFKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEV 240

Query: 241 ASSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTV 300
           ASSL GALKVGRIDC+SES LCK+LGIYPHR P+IFVYSYIKST   EGSLVEY+GD  V
Sbjct: 241 ASSLQGALKVGRIDCDSESSLCKDLGIYPHRTPRIFVYSYIKST---EGSLVEYSGDVAV 300

Query: 301 KNLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQF 360
           KNL+SFCQEH PRFSQRVNL QFDFSSS+G  LPTLMLLSTKKDTPVIWRVLSGL+RKQF
Sbjct: 301 KNLRSFCQEHFPRFSQRVNLKQFDFSSSDGGRLPTLMLLSTKKDTPVIWRVLSGLFRKQF 360

Query: 361 SFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLND 420
           SFYD EVTDVSDP++KKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLSNLLN 
Sbjct: 361 SFYDTEVTDVSDPSVKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNG 420

Query: 421 FEKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLEL 480
           FEKKNRK  SRPASKT SDS ENQIPLLT SNFD+VCGEKTPVCVIGAFRSSKARNKLEL
Sbjct: 421 FEKKNRKAVSRPASKTQSDSAENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLEL 480

Query: 481 ILNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRK 540
           ILN VSQKTLSR    TPGSK++ISYAL+DA+KQPSFLNAFD AG+KSSDKILIAYKRRK
Sbjct: 481 ILNMVSQKTLSRRQNSTPGSKETISYALVDASKQPSFLNAFDGAGFKSSDKILIAYKRRK 540

Query: 541 GKFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           GKFAAHLGE+TEEEVE+FIGSVLNGDV F+KTRQ P+LK
Sbjct: 541 GKFAAHLGEITEEEVEKFIGSVLNGDVTFTKTRQKPILK 575

BLAST of Lag0027288 vs. NCBI nr
Match: XP_022935557.1 (dnaJ protein ERDJ3A [Cucurbita moschata] >KAG6580462.1 DnaJ protein ERDJ3A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017212.1 DnaJ protein ERDJ3A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 932.6 bits (2409), Expect = 1.7e-267
Identity = 485/578 (83.91%), Postives = 525/578 (90.83%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           ME RL+P +LIMASLFLFLLS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD++GDEKGSPGFGPG+SGDQGGG+TFFTNEGR
Sbjct: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120

Query: 121 RQGQQTFRPGWQSTGGQGGSQSFSFSFGGPT-GSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F+P WQ TGGQGGSQSFSFSFGGP+ GSNPF  FGMEDIFSN FGGGFKDEG+F
Sbjct: 121 RQGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPF-GFGMEDIFSNFFGGGFKDEGNF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           GS    KAQSGSKSSS I+V+N QVFK+E+V QG+TWLLFP T SLKG DHVQSI+E+VA
Sbjct: 181 GSH--FKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           SSL GALK GRIDCESES LCK+LGIYPHR P+IFVYSYIKST   +GSLVEY+GD  VK
Sbjct: 241 SSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKST---DGSLVEYSGDVAVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LKSFCQEHLPRFS+RV+L QFDFSS+NG  LPTLMLLSTKKDTPVIWRVLSG+Y KQFS
Sbjct: 301 SLKSFCQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFS 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLS L+N F
Sbjct: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK AS+PASKT SDS +NQIPLLT  NFD++CG+ TPVCVIGAFRSSKARNKLE+I
Sbjct: 421 EKKNRKAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKG 540
           LN VSQKTLSR     PGSK+SISY+L+DATKQPSFL AFDSAG+KSSD+ILIAYKRRK 
Sbjct: 481 LNMVSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKE 540

Query: 541 KFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           KFAAHLGEMTEEEVE+FIGSVLNGD+ F+KTRQ PVLK
Sbjct: 541 KFAAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Lag0027288 vs. NCBI nr
Match: TYK24944.1 (dnaJ protein ERDJ3A [Cucumis melo var. makuwa])

HSP 1 Score: 930.6 bits (2404), Expect = 6.4e-267
Identity = 489/578 (84.60%), Postives = 522/578 (90.31%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLA I+L++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKKGAQQKFEEINNAYEILSDEEKRKNFDM+GDEKG+PGFG G SGDQGGGYTFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQTFRPG-WQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F PG WQSTGGQGGSQSFSFSFGGP+GSNPF  FGMEDIFSN FGGGFKD+GSF
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPF-GFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           G  G SKAQSG KSSSSI+VIN Q+ KKEIVDQGMTWLLFP+T SLKG DHVQSI+EEVA
Sbjct: 181 G--GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           S+L GALKVGRIDC+SES  CK+LGIYPHR P+IFVYSYIKS   +EGSLVEY+GD  VK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKS---NEGSLVEYSGDIDVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LK FCQEH PRFSQRVNL QFDFSSSN   LPTLMLLSTKK+TPVIWRVLSGL+RK F+
Sbjct: 301 SLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFN 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLSNLLN F
Sbjct: 361 FYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK ASRP SKT SDS ENQIPLLT SNFD+VCGEKTPVCVIGAFRSSKARNKLE I
Sbjct: 421 EKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKG 540
           L  VSQKTLSR    TPGSK++ISYAL+DATKQ SFLNAFDSAG+KSSDKILIAYKRRKG
Sbjct: 481 LKMVSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKG 540

Query: 541 KFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           KFAA+LGE+TEEEVE+FIGSVLNGDV F+ TRQ P+LK
Sbjct: 541 KFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of Lag0027288 vs. NCBI nr
Match: XP_023529005.1 (dnaJ protein ERDJ3A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 926.8 bits (2394), Expect = 9.2e-266
Identity = 482/578 (83.39%), Postives = 523/578 (90.48%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           ME RL+P +LIMASLFLFLLS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD++GDEKGSPGFGP +SGDQGGG+TFFTNEGR
Sbjct: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPDYSGDQGGGHTFFTNEGR 120

Query: 121 RQGQQTFRPGWQSTGGQGGSQSFSFSFGGPT-GSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F+P WQ TGGQGGSQSFSFSFGGP+ GSNPF  FGMEDIFSN FGGGFKDEG+F
Sbjct: 121 RQGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPF-GFGMEDIFSNFFGGGFKDEGNF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           GS    KAQSGSKSSS I+V+N QVFK+E+V QG+TWLLFP T SLKG DHVQSI+E+VA
Sbjct: 181 GSH--FKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           SSL GALK GRIDCESES LCK+LGIYPHR P+IFVYSYIKST   +GSLVEY+GD  VK
Sbjct: 241 SSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKST---DGSLVEYSGDVAVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           NLKSFCQEH PRFS+RV+L QFDFSS+NG  LPTLMLLSTKKDTPVIWRVLSG+Y KQFS
Sbjct: 301 NLKSFCQEHFPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFS 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLS LL+ F
Sbjct: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLLDGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK AS+PASKT SDS +N+IPLLT SNFD++CG+ TPVCVIGAF+SSKARNKLE+I
Sbjct: 421 EKKNRKAASKPASKTQSDSAQNRIPLLTGSNFDSICGKNTPVCVIGAFKSSKARNKLEMI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKG 540
           LN VSQKTLSR     P SK+SISY+L+DATKQPSFL AFDSAG+KSSD+ILIAYKRRK 
Sbjct: 481 LNMVSQKTLSRRQISAPSSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKE 540

Query: 541 KFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           KFAAHLGEMTEEEVE+FIGSVLNGD+ F+KTRQ PVLK
Sbjct: 541 KFAAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Lag0027288 vs. NCBI nr
Match: XP_004137701.1 (dnaJ protein ERDJ3A isoform X1 [Cucumis sativus] >KGN58737.1 hypothetical protein Csa_001124 [Cucumis sativus])

HSP 1 Score: 926.0 bits (2392), Expect = 1.6e-265
Identity = 486/578 (84.08%), Postives = 520/578 (89.97%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           METRLA I+ +MA LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   METRLASIIFVMAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
            KKGAQQKFEEINNAYEILSDEEKRKNFDM+GDEKG+PGFGPG SGDQGGGYTFFTN G 
Sbjct: 61  NKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGPGSSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQTFRPG-WQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F PG WQSTGGQGGSQSFSFSFGGP+GSNPF  FG EDIFSNLFGGGF+ +GSF
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPF-GFGAEDIFSNLFGGGFQGKGSF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           G  G SKAQSG KSSSSI+VIN +  KKEIVDQGMTWLLFP+  SLKG DHVQS +EEVA
Sbjct: 181 G--GHSKAQSGPKSSSSIKVINKKSLKKEIVDQGMTWLLFPTASSLKGLDHVQSTIEEVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           +SL GALKVGRIDC+SES  CK+LGIYPHR P+IFVYSYIKS   SEGSLVEY+GD  VK
Sbjct: 241 NSLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKS---SEGSLVEYSGDIAVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LKSFCQEH PRFSQRVNL QFDFSSSN  GLPTLMLLSTKK+TPVIWRVLSGL+RKQF+
Sbjct: 301 SLKSFCQEHFPRFSQRVNLKQFDFSSSNRGGLPTLMLLSTKKETPVIWRVLSGLFRKQFN 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVT  SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLSNLLN F
Sbjct: 361 FYDAEVTYASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK ASRPAS+T SDS ENQIPLL  SNFD+VCGEKTPVCVIGAFRSSKARNKLE I
Sbjct: 421 EKKNRKAASRPASRTQSDSGENQIPLLAGSNFDSVCGEKTPVCVIGAFRSSKARNKLESI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKG 540
           LN VSQKTLSR    TPGSK+++SYAL+DATKQ SFLNAFDSAG+KSSDKILIAYKRRKG
Sbjct: 481 LNMVSQKTLSRRQNSTPGSKETVSYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKG 540

Query: 541 KFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           KFAA+L E+TEEEVE+FIGSVLNGDV F+KT Q P+LK
Sbjct: 541 KFAAYLDEITEEEVEKFIGSVLNGDVTFTKTHQKPILK 572

BLAST of Lag0027288 vs. ExPASy Swiss-Prot
Match: Q9SR96 (DnaJ protein ERDJ3A OS=Arabidopsis thaliana OX=3702 GN=ERDJ3A PE=1 SV=1)

HSP 1 Score: 631.7 bits (1628), Expect = 8.0e-180
Identity = 342/583 (58.66%), Postives = 446/583 (76.50%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           + TRLA  V+++++  L LL+++AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKN
Sbjct: 2   VRTRLAISVVLVST--LLLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKN 61

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           K KGAQ+KF EINNAYEILSDEEKRKN+D++GDEKG PGF  GF G   GGY++ ++ G 
Sbjct: 62  KDKGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGGYSYSSSGG- 121

Query: 121 RQGQQTFRP-GWQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
             G     P GWQ+ GG GGS+SFSFSFGGP+ S+    FGM+DIFS   GG  K +  F
Sbjct: 122 --GFNFGGPGGWQNMGGGGGSKSFSFSFGGPSESS--FGFGMDDIFSMFSGGSSKGKEQF 181

Query: 181 GSFG-GSKAQSGSKSS--SSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIME 240
           G FG  S A+S SKSS  ++I+ IN+QV+KK++VDQGMTWLL    PS +G  + +SI+E
Sbjct: 182 GGFGSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIE 241

Query: 241 EVASSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDF 300
           EVA SL GALKVGR++CE+ES LCK LGI P R P++FVYSY   T   + +L EY  + 
Sbjct: 242 EVAESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSY---TSSGKATLAEYTEEL 301

Query: 301 TVKNLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRK 360
             K +KSFCQEHLPRFS++++LN FD S+ + +  P ++LLSTKKDTPVIWRVLSGLY  
Sbjct: 302 VAKKVKSFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNG 361

Query: 361 QFSFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLL 420
           +F FY+ EV D SDP I+KLGVD  PAIVGWLSNGE+ VL+TGI VK+L+SA+ +L  LL
Sbjct: 362 RFVFYNTEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLL 421

Query: 421 NDFEKKNRKEASR-PASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNK 480
              EKKN+K +S+  A + P++S+E +IPLL++ NFD++CGE TPVC+IGAFRSS  + K
Sbjct: 422 EGLEKKNKKVSSKSQAGQAPNESSE-KIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEK 481

Query: 481 LELILNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYK-SSDKILIAY 540
           L+ I++ VSQK+LSR    T GS+D++SY+L+DATKQ +FL++ D + +K SSDK+LIAY
Sbjct: 482 LQSIMSKVSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTSSDKLLIAY 541

Query: 541 KRRKGKFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           K R+GKFA   G+MT EEVE+F+ +VLNGD+ F+KTRQ P +K
Sbjct: 542 KPRRGKFATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of Lag0027288 vs. ExPASy Swiss-Prot
Match: Q10MW6 (DnaJ protein ERDJ3A OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ3A PE=1 SV=2)

HSP 1 Score: 567.0 bits (1460), Expect = 2.4e-160
Identity = 306/581 (52.67%), Postives = 415/581 (71.43%), Query Frame = 0

Query: 10  LIMASLFLFLLSLE---AKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQ 69
           L++AS+ L  ++L    AK +DPYKVLGV+++ASQR+IQKAFHKLSL+YHPDKNK KGAQ
Sbjct: 8   LLVASIVLSSIALHVAAAKNLDPYKVLGVDKSASQRDIQKAFHKLSLKYHPDKNKSKGAQ 67

Query: 70  QKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGRRQGQQT 129
           +KF EINNAY+ILSDEEKRKN+D++GDEKG+PGFG G  G++  GYT+FT  G +    +
Sbjct: 68  EKFAEINNAYDILSDEEKRKNYDLYGDEKGNPGFGGGNFGNR-EGYTYFTGGGAKTSHFS 127

Query: 130 FRPGWQSTGGQGGSQSFSFSFGGPT----GSNPFSSFGMEDIFSNLFGGGFKDEGSFGSF 189
              GWQ+ GGQG +++FSFSFGG      G NPF +F   D+FSN+F GG    GS  + 
Sbjct: 128 SGDGWQTMGGQGNTKTFSFSFGGGNPGAGGGNPF-NFDFGDVFSNIFSGGSMG-GSQHTG 187

Query: 190 GGSKAQSGSKSSS------SIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIME 249
              KA+ G+KSS       +I+ +  Q+F KE  DQG+TWLL   TP+ KG   ++S++E
Sbjct: 188 SAGKARRGTKSSGHDSSSVNIQEVTMQIFNKETADQGITWLLLFYTPNTKGQFVLESVVE 247

Query: 250 EVASSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDF 309
           +VA SL GAL+ G+++C+ E  LCK  G+   ++ ++F+YSY   T   +GSL EY+GD+
Sbjct: 248 DVARSLDGALRAGKVNCDHEKALCKKAGVSIGKSARLFIYSY---TTTEKGSLHEYSGDY 307

Query: 310 TVKNLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRK 369
             K+LK+FCQEHLPRFS+RV++NQF F S+    LP ++LLS KKDTP +WR +SG++R 
Sbjct: 308 DSKSLKTFCQEHLPRFSKRVDINQFSFPSNIIPNLPQVLLLSAKKDTPAMWRAVSGMFRS 367

Query: 370 QFSFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLL 429
           +  FYDAEV DVS P +K LGV  +PA++G   NGE  +L+ GI+VKDLRS I +L NLL
Sbjct: 368 RLIFYDAEVQDVSHPLLKSLGVKNIPALIGRSVNGEEQLLKDGISVKDLRSGIKELKNLL 427

Query: 430 NDFEKKNRKEASRPASKT--PSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARN 489
            +FEKKN+K AS  A K        EN+IPLLT SNF+ +CGEKT VC++G F+SSKA+ 
Sbjct: 428 ENFEKKNKKLASNQAKKPAHTDQPKENKIPLLTASNFEEICGEKTSVCILGIFKSSKAKE 487

Query: 490 KLELILNTVSQKTLSR--TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKR 549
            LE +L+ +SQKTL R     S ++++YAL+D  KQ +FL+ FD + +KSSDK+L+AYK 
Sbjct: 488 NLEAVLSEISQKTLIRGQNYNSGNAVAYALLDGNKQSAFLSTFDKSAFKSSDKLLLAYKP 547

Query: 550 RKGKFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           R+G++A +  E+T EE ERF+ SVLNGDV  S   + PVL+
Sbjct: 548 RRGRYAVYDNEVTMEEAERFVVSVLNGDVQLSAAGRKPVLR 582

BLAST of Lag0027288 vs. ExPASy Swiss-Prot
Match: B1HUD0 (Chaperone protein DnaJ OS=Lysinibacillus sphaericus (strain C3-41) OX=444177 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 9.3e-19
Identity = 53/108 (49.07%), Postives = 72/108 (66.67%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKN 87
           D Y+VLG+ ++A++ EI+KA+ KLS QYHPD NK+ GA +KF+EI  AYE+LSD++K+  
Sbjct: 5   DYYEVLGLTKSATKDEIKKAYRKLSKQYHPDLNKEPGADEKFKEIAEAYEVLSDDQKKAR 64

Query: 88  FDMFGDEKGSPGFGPGFSGDQGGGY-----TFFTNEGRRQGQQTFRPG 131
           +D FG E  + GFG GF G   GG+     +FF   GRRQ     R G
Sbjct: 65  YDQFGHEDPNAGFGGGFGGGGFGGFEDIFSSFFGGGGRRQDPNAPRKG 112

BLAST of Lag0027288 vs. ExPASy Swiss-Prot
Match: Q8EUM4 (Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.7e-18
Identity = 64/151 (42.38%), Postives = 84/151 (55.63%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKN 87
           D Y+VLGV ++A+  +I+ AF K ++QYHPD+NK+  A++KF+E+N AYE+LSD +KR N
Sbjct: 6   DYYEVLGVSKDATDDQIKSAFRKKAMQYHPDRNKEPDAEEKFKEVNQAYEVLSDPDKRAN 65

Query: 88  FDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGRRQGQQTFRPGWQSTGGQGGSQSFSFSF 147
           +D FG E        G  G  GGG  F   +   Q            GG+GG   F  SF
Sbjct: 66  YDRFGHE--------GVDGQFGGGAGFDPFDIFNQ---------FFGGGRGGGAHFEQSF 125

Query: 148 GGPTGSNPFSSFGMEDIFSNLFGGGFKDEGS 179
           GG          G EDIFSN FGGG +   S
Sbjct: 126 GGS---------GFEDIFSNFFGGGRRGASS 130

BLAST of Lag0027288 vs. ExPASy Swiss-Prot
Match: Q49Y21 (Chaperone protein DnaJ OS=Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41) OX=342451 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 1.3e-17
Identity = 66/146 (45.21%), Postives = 81/146 (55.48%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKN 87
           D Y+VLGV ++AS+ EI+KA+ KLS QYHPD NK++GA +KF+EI+ AYE+LSDE KR N
Sbjct: 5   DYYEVLGVSKSASKDEIKKAYRKLSKQYHPDINKEEGADEKFKEISEAYEVLSDENKRAN 64

Query: 88  FDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGRRQGQQTFRPGWQSTGGQG-GSQSFSFS 147
           +D FG +           G QGG                        GGQG G Q FS  
Sbjct: 65  YDQFGHD-----------GPQGG-----------------------FGGQGFGGQDFS-G 106

Query: 148 FGGPTGSNPFSSFGMEDIFSNLFGGG 173
           FGG          G EDIFS+ FGGG
Sbjct: 125 FGGG---------GFEDIFSSFFGGG 106

BLAST of Lag0027288 vs. ExPASy TrEMBL
Match: A0A6J1F5W9 (dnaJ protein ERDJ3A OS=Cucurbita moschata OX=3662 GN=LOC111442396 PE=4 SV=1)

HSP 1 Score: 932.6 bits (2409), Expect = 8.1e-268
Identity = 485/578 (83.91%), Postives = 525/578 (90.83%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           ME RL+P +LIMASLFLFLLS EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MEIRLSPFILIMASLFLFLLS-EAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKK AQ KFEEINNAYEILSDEEKRKNFD++GDEKGSPGFGPG+SGDQGGG+TFFTNEGR
Sbjct: 61  KKKSAQHKFEEINNAYEILSDEEKRKNFDLYGDEKGSPGFGPGYSGDQGGGHTFFTNEGR 120

Query: 121 RQGQQTFRPGWQSTGGQGGSQSFSFSFGGPT-GSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F+P WQ TGGQGGSQSFSFSFGGP+ GSNPF  FGMEDIFSN FGGGFKDEG+F
Sbjct: 121 RQGQQNFKPQWQGTGGQGGSQSFSFSFGGPSGGSNPF-GFGMEDIFSNFFGGGFKDEGNF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           GS    KAQSGSKSSS I+V+N QVFK+E+V QG+TWLLFP T SLKG DHVQSI+E+VA
Sbjct: 181 GSH--FKAQSGSKSSSIIKVVNKQVFKQEVVGQGVTWLLFPITSSLKGVDHVQSIIEDVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           SSL GALK GRIDCESES LCK+LGIYPHR P+IFVYSYIKST   +GSLVEY+GD  VK
Sbjct: 241 SSLQGALKAGRIDCESESSLCKDLGIYPHRTPRIFVYSYIKST---DGSLVEYSGDVAVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LKSFCQEHLPRFS+RV+L QFDFSS+NG  LPTLMLLSTKKDTPVIWRVLSG+Y KQFS
Sbjct: 301 SLKSFCQEHLPRFSRRVDLRQFDFSSNNGGRLPTLMLLSTKKDTPVIWRVLSGMYNKQFS 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLS L+N F
Sbjct: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSYLVNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK AS+PASKT SDS +NQIPLLT  NFD++CG+ TPVCVIGAFRSSKARNKLE+I
Sbjct: 421 EKKNRKAASKPASKTQSDSAQNQIPLLTGFNFDSICGKNTPVCVIGAFRSSKARNKLEMI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKG 540
           LN VSQKTLSR     PGSK+SISY+L+DATKQPSFL AFDSAG+KSSD+ILIAYKRRK 
Sbjct: 481 LNMVSQKTLSRRQISAPGSKESISYSLVDATKQPSFLKAFDSAGFKSSDQILIAYKRRKE 540

Query: 541 KFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           KFAAHLGEMTEEEVE+FIGSVLNGD+ F+KTRQ PVLK
Sbjct: 541 KFAAHLGEMTEEEVEKFIGSVLNGDITFTKTRQKPVLK 571

BLAST of Lag0027288 vs. ExPASy TrEMBL
Match: A0A5D3DMX5 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G00600 PE=4 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 3.1e-267
Identity = 489/578 (84.60%), Postives = 522/578 (90.31%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLA I+L++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKKGAQQKFEEINNAYEILSDEEKRKNFDM+GDEKG+PGFG G SGDQGGGYTFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQTFRPG-WQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F PG WQSTGGQGGSQSFSFSFGGP+GSNPF  FGMEDIFSN FGGGFKD+GSF
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPF-GFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           G  G SKAQSG KSSSSI+VIN Q+ KKEIVDQGMTWLLFP+T SLKG DHVQSI+EEVA
Sbjct: 181 G--GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           S+L GALKVGRIDC+SES  CK+LGIYPHR P+IFVYSYIKS   +EGSLVEY+GD  VK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKS---NEGSLVEYSGDIDVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LK FCQEH PRFSQRVNL QFDFSSSN   LPTLMLLSTKK+TPVIWRVLSGL+RK F+
Sbjct: 301 SLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFN 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLSNLLN F
Sbjct: 361 FYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK ASRP SKT SDS ENQIPLLT SNFD+VCGEKTPVCVIGAFRSSKARNKLE I
Sbjct: 421 EKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKG 540
           L  VSQKTLSR    TPGSK++ISYAL+DATKQ SFLNAFDSAG+KSSDKILIAYKRRKG
Sbjct: 481 LKMVSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKG 540

Query: 541 KFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           KFAA+LGE+TEEEVE+FIGSVLNGDV F+ TRQ P+LK
Sbjct: 541 KFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 572

BLAST of Lag0027288 vs. ExPASy TrEMBL
Match: A0A5A7TMT4 (DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G005460 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 7.6e-266
Identity = 489/579 (84.46%), Postives = 522/579 (90.16%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLA I+L++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKKGAQQKFEEINNAYEILSDEEKRKNFDM+GDEKG+PGFG G SGDQGGGYTFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQTFRPG-WQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F PG WQSTGGQGGSQSFSFSFGGP+GSNPF  FGMEDIFSN FGGGFKD+GSF
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPF-GFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           G  G SKAQSG KSSSSI+VIN Q+ KKEIVDQGMTWLLFP+T SLKG DHVQSI+EEVA
Sbjct: 181 G--GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           S+L GALKVGRIDC+SES  CK+LGIYPHR P+IFVYSYIKS   +EGSLVEY+GD  VK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKS---NEGSLVEYSGDIDVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LK FCQEH PRFSQRVNL QFDFSSSN   LPTLMLLSTKK+TPVIWRVLSGL+RK F+
Sbjct: 301 SLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFN 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLSNLLN F
Sbjct: 361 FYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK ASRP SKT SDS ENQIPLLT SNFD+VCGEKTPVCVIGAFRSSKARNKLE I
Sbjct: 421 EKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAF-DSAGYKSSDKILIAYKRRK 540
           L  VSQKTLSR    TPGSK++ISYAL+DATKQ SFLNAF DSAG+KSSDKILIAYKRRK
Sbjct: 481 LKMVSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDKILIAYKRRK 540

Query: 541 GKFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           GKFAA+LGE+TEEEVE+FIGSVLNGDV F+ TRQ P+LK
Sbjct: 541 GKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of Lag0027288 vs. ExPASy TrEMBL
Match: A0A0A0LDD2 (J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G730990 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 7.6e-266
Identity = 486/578 (84.08%), Postives = 520/578 (89.97%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           METRLA I+ +MA LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   METRLASIIFVMAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
            KKGAQQKFEEINNAYEILSDEEKRKNFDM+GDEKG+PGFGPG SGDQGGGYTFFTN G 
Sbjct: 61  NKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGPGSSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQTFRPG-WQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F PG WQSTGGQGGSQSFSFSFGGP+GSNPF  FG EDIFSNLFGGGF+ +GSF
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPF-GFGAEDIFSNLFGGGFQGKGSF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           G  G SKAQSG KSSSSI+VIN +  KKEIVDQGMTWLLFP+  SLKG DHVQS +EEVA
Sbjct: 181 G--GHSKAQSGPKSSSSIKVINKKSLKKEIVDQGMTWLLFPTASSLKGLDHVQSTIEEVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           +SL GALKVGRIDC+SES  CK+LGIYPHR P+IFVYSYIKS   SEGSLVEY+GD  VK
Sbjct: 241 NSLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKS---SEGSLVEYSGDIAVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LKSFCQEH PRFSQRVNL QFDFSSSN  GLPTLMLLSTKK+TPVIWRVLSGL+RKQF+
Sbjct: 301 SLKSFCQEHFPRFSQRVNLKQFDFSSSNRGGLPTLMLLSTKKETPVIWRVLSGLFRKQFN 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVT  SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLSNLLN F
Sbjct: 361 FYDAEVTYASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK ASRPAS+T SDS ENQIPLL  SNFD+VCGEKTPVCVIGAFRSSKARNKLE I
Sbjct: 421 EKKNRKAASRPASRTQSDSGENQIPLLAGSNFDSVCGEKTPVCVIGAFRSSKARNKLESI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYKSSDKILIAYKRRKG 540
           LN VSQKTLSR    TPGSK+++SYAL+DATKQ SFLNAFDSAG+KSSDKILIAYKRRKG
Sbjct: 481 LNMVSQKTLSRRQNSTPGSKETVSYALVDATKQVSFLNAFDSAGFKSSDKILIAYKRRKG 540

Query: 541 KFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           KFAA+L E+TEEEVE+FIGSVLNGDV F+KT Q P+LK
Sbjct: 541 KFAAYLDEITEEEVEKFIGSVLNGDVTFTKTHQKPILK 572

BLAST of Lag0027288 vs. ExPASy TrEMBL
Match: A0A1S3B542 (dnaJ protein ERDJ3A OS=Cucumis melo OX=3656 GN=LOC103486271 PE=4 SV=1)

HSP 1 Score: 926.0 bits (2392), Expect = 7.6e-266
Identity = 489/579 (84.46%), Postives = 522/579 (90.16%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           M TRLA I+L++A LFLF LSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN
Sbjct: 1   MGTRLASIILVLAFLFLFRLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           KKKGAQQKFEEINNAYEILSDEEKRKNFDM+GDEKG+PGFG G SGDQGGGYTFFTN G 
Sbjct: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMYGDEKGAPGFGAGPSGDQGGGYTFFTNGGG 120

Query: 121 RQGQQTFRPG-WQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
           RQGQQ F PG WQSTGGQGGSQSFSFSFGGP+GSNPF  FGMEDIFSN FGGGFKD+GSF
Sbjct: 121 RQGQQPFGPGQWQSTGGQGGSQSFSFSFGGPSGSNPF-GFGMEDIFSNFFGGGFKDKGSF 180

Query: 181 GSFGGSKAQSGSKSSSSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIMEEVA 240
           G  G SKAQSG KSSSSI+VIN Q+ KKEIVDQGMTWLLFP+T SLKG DHVQSI+EEVA
Sbjct: 181 G--GHSKAQSGPKSSSSIKVINKQLLKKEIVDQGMTWLLFPTTSSLKGVDHVQSIIEEVA 240

Query: 241 SSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDFTVK 300
           S+L GALKVGRIDC+SES  CK+LGIYPHR P+IFVYSYIKS   +EGSLVEY+GD  VK
Sbjct: 241 STLQGALKVGRIDCDSESSFCKDLGIYPHRTPRIFVYSYIKS---NEGSLVEYSGDIDVK 300

Query: 301 NLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRKQFS 360
           +LK FCQEH PRFSQRVNL QFDFSSSN   LPTLMLLSTKK+TPVIWRVLSGL+RK F+
Sbjct: 301 SLKGFCQEHFPRFSQRVNLKQFDFSSSNRGRLPTLMLLSTKKETPVIWRVLSGLFRKLFN 360

Query: 361 FYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLLNDF 420
           FYDAEVTD SDP+IKKLGVDALPAIVGWLSNGERHVLRTGINVKDL+SAIDDLSNLLN F
Sbjct: 361 FYDAEVTDASDPSIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLKSAIDDLSNLLNGF 420

Query: 421 EKKNRKEASRPASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNKLELI 480
           EKKNRK ASRP SKT SDS ENQIPLLT SNFD+VCGEKTPVCVIGAFRSSKARNKLE I
Sbjct: 421 EKKNRKAASRPTSKTQSDSVENQIPLLTGSNFDSVCGEKTPVCVIGAFRSSKARNKLESI 480

Query: 481 LNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAF-DSAGYKSSDKILIAYKRRK 540
           L  VSQKTLSR    TPGSK++ISYAL+DATKQ SFLNAF DSAG+KSSDKILIAYKRRK
Sbjct: 481 LKMVSQKTLSRRQNSTPGSKETISYALVDATKQVSFLNAFVDSAGFKSSDKILIAYKRRK 540

Query: 541 GKFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           GKFAA+LGE+TEEEVE+FIGSVLNGDV F+ TRQ P+LK
Sbjct: 541 GKFAAYLGEITEEEVEKFIGSVLNGDVTFTNTRQKPILK 573

BLAST of Lag0027288 vs. TAIR 10
Match: AT3G08970.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 631.7 bits (1628), Expect = 5.7e-181
Identity = 342/583 (58.66%), Postives = 446/583 (76.50%), Query Frame = 0

Query: 1   METRLAPIVLIMASLFLFLLSLEAKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN 60
           + TRLA  V+++++  L LL+++AK++DPYKVLGV ++A QREIQKAFHK SL+YHPDKN
Sbjct: 2   VRTRLAISVVLVST--LLLLNVKAKSVDPYKVLGVSKDAKQREIQKAFHKQSLKYHPDKN 61

Query: 61  KKKGAQQKFEEINNAYEILSDEEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGR 120
           K KGAQ+KF EINNAYEILSDEEKRKN+D++GDEKG PGF  GF G   GGY++ ++ G 
Sbjct: 62  KDKGAQEKFAEINNAYEILSDEEKRKNYDLYGDEKGQPGFDSGFPGG-NGGYSYSSSGG- 121

Query: 121 RQGQQTFRP-GWQSTGGQGGSQSFSFSFGGPTGSNPFSSFGMEDIFSNLFGGGFKDEGSF 180
             G     P GWQ+ GG GGS+SFSFSFGGP+ S+    FGM+DIFS   GG  K +  F
Sbjct: 122 --GFNFGGPGGWQNMGGGGGSKSFSFSFGGPSESS--FGFGMDDIFSMFSGGSSKGKEQF 181

Query: 181 GSFG-GSKAQSGSKSS--SSIRVINTQVFKKEIVDQGMTWLLFPSTPSLKGFDHVQSIME 240
           G FG  S A+S SKSS  ++I+ IN+QV+KK++VDQGMTWLL    PS +G  + +SI+E
Sbjct: 182 GGFGSSSNAESKSKSSTVAAIKTINSQVYKKDVVDQGMTWLLLSYLPSQRGSQYHESIIE 241

Query: 241 EVASSLLGALKVGRIDCESESFLCKNLGIYPHRNPKIFVYSYIKSTEGSEGSLVEYNGDF 300
           EVA SL GALKVGR++CE+ES LCK LGI P R P++FVYSY   T   + +L EY  + 
Sbjct: 242 EVAESLQGALKVGRLNCETESSLCKQLGIVPRRAPRMFVYSY---TSSGKATLAEYTEEL 301

Query: 301 TVKNLKSFCQEHLPRFSQRVNLNQFDFSSSNGRGLPTLMLLSTKKDTPVIWRVLSGLYRK 360
             K +KSFCQEHLPRFS++++LN FD S+ + +  P ++LLSTKKDTPVIWRVLSGLY  
Sbjct: 302 VAKKVKSFCQEHLPRFSKKIDLNTFDVSAVSSQKTPKVLLLSTKKDTPVIWRVLSGLYNG 361

Query: 361 QFSFYDAEVTDVSDPTIKKLGVDALPAIVGWLSNGERHVLRTGINVKDLRSAIDDLSNLL 420
           +F FY+ EV D SDP I+KLGVD  PAIVGWLSNGE+ VL+TGI VK+L+SA+ +L  LL
Sbjct: 362 RFVFYNTEVHDTSDPKIQKLGVDKFPAIVGWLSNGEKQVLKTGITVKNLKSAVQELGKLL 421

Query: 421 NDFEKKNRKEASR-PASKTPSDSTENQIPLLTKSNFDAVCGEKTPVCVIGAFRSSKARNK 480
              EKKN+K +S+  A + P++S+E +IPLL++ NFD++CGE TPVC+IGAFRSS  + K
Sbjct: 422 EGLEKKNKKVSSKSQAGQAPNESSE-KIPLLSRPNFDSICGENTPVCIIGAFRSSNGKEK 481

Query: 481 LELILNTVSQKTLSR----TPGSKDSISYALIDATKQPSFLNAFDSAGYK-SSDKILIAY 540
           L+ I++ VSQK+LSR    T GS+D++SY+L+DATKQ +FL++ D + +K SSDK+LIAY
Sbjct: 482 LQSIMSKVSQKSLSRRQASTTGSQDTVSYSLLDATKQSAFLSSLDKSEFKTSSDKLLIAY 541

Query: 541 KRRKGKFAAHLGEMTEEEVERFIGSVLNGDVIFSKTRQNPVLK 574
           K R+GKFA   G+MT EEVE+F+ +VLNGD+ F+KTRQ P +K
Sbjct: 542 KPRRGKFATFKGDMTIEEVEKFVAAVLNGDIQFTKTRQKPQIK 572

BLAST of Lag0027288 vs. TAIR 10
Match: AT2G22360.1 (DNAJ heat shock family protein )

HSP 1 Score: 85.9 bits (211), Expect = 1.2e-16
Identity = 42/78 (53.85%), Postives = 59/78 (75.64%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKN 87
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK  GA++KF+EI+NAYE+LSD+EK+  
Sbjct: 86  DYYSVLGVSKNATKAEIKSAYRKLARNYHPDVNKDPGAEEKFKEISNAYEVLSDDEKKSL 145

Query: 88  FDMFGDE--KGSPGFGPG 104
           +D +G+   KG+ GFG G
Sbjct: 146 YDRYGEAGLKGAAGFGNG 163

BLAST of Lag0027288 vs. TAIR 10
Match: AT4G39960.1 (Molecular chaperone Hsp40/DnaJ family protein )

HSP 1 Score: 77.8 bits (190), Expect = 3.2e-14
Identity = 65/176 (36.93%), Postives = 90/176 (51.14%), Query Frame = 0

Query: 28  DPYKVLGVERNASQREIQKAFHKLSLQYHPDKNKKKGAQQKFEEINNAYEILSDEEKRKN 87
           D Y VLGV +NA++ EI+ A+ KL+  YHPD NK  GA+ KF+EI+NAYEILSD+EKR  
Sbjct: 85  DFYSVLGVSKNATKAEIKSAYRKLARSYHPDVNKDAGAEDKFKEISNAYEILSDDEKRSL 144

Query: 88  FDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGRRQGQQTFRPGWQSTGGQGGSQSFSFSF 147
           +D +G E G  G G G  GD    +  F         ++   G    GG GG        
Sbjct: 145 YDRYG-EAGVKGAGMGGMGDYSNPFDLF---------ESLFEGMGGMGGMGGGM------ 204

Query: 148 GGPTGSNPFSSFGMEDIFSNLFG-----GGFKDE------GSFGSFGGSKAQSGSK 193
            G  GS   +  G ++ +S +        G + E       S G+  GS A++G+K
Sbjct: 205 -GSRGSRSRAIDGEDEYYSLILNFKEAVFGIEKEIEISRLESCGTCNGSGAKAGTK 243

BLAST of Lag0027288 vs. TAIR 10
Match: AT5G03160.1 (homolog of mamallian P58IPK )

HSP 1 Score: 77.4 bits (189), Expect = 4.1e-14
Identity = 58/141 (41.13%), Postives = 73/141 (51.77%), Query Frame = 0

Query: 24  AKTMDPYKVLGVERNASQREIQKAFHKLSLQYHPDKN--KKKGAQQKFEEINNAYEILSD 83
           +K  D YK+LG+ R AS  EI+KA+ KL+LQ+HPDKN   ++ A+ KF EI  AYEIL D
Sbjct: 366 SKRKDWYKILGISRTASISEIKKAYKKLALQWHPDKNVGNREEAENKFREIAAAYEILGD 425

Query: 84  EEKRKNFDMFGDEKGSPGFGPGFSGDQGGGYTFFTNEGRRQGQQTFRPGWQSTGGQGGSQ 143
           ++KR  FD   D +   G G       GGGY  F                   GG GG Q
Sbjct: 426 DDKRARFDRGEDLEDMGGGG-------GGGYNPF------------------HGGGGGGQ 481

Query: 144 SFSFSFGG--PTGSNPFSSFG 161
            ++F F G  P G   F  FG
Sbjct: 486 QYTFHFEGGFPGGGGGFGGFG 481

BLAST of Lag0027288 vs. TAIR 10
Match: AT3G08910.1 (DNAJ heat shock family protein )

HSP 1 Score: 75.1 bits (183), Expect = 2.0e-13
Identity = 57/151 (37.75%), Postives = 84/151 (55.63%), Query Frame = 0

Query: 27  MDPYKVLGVERNASQREIQKAFHKLSLQYHPDK--NKKKGAQQKFEEINNAYEILSDEEK 86
           +D YKVL V+RNA   +++KA+ KL++++HPDK  N KK A+ KF++I+ AY++LSD +K
Sbjct: 3   VDYYKVLQVDRNAKDDDLKKAYRKLAMKWHPDKNPNNKKDAEAKFKQISEAYDVLSDPQK 62

Query: 87  RKNFDMFGDE-----KGSPGFGPGFSGDQGGGYTFFTNEGRRQGQQTFRPGWQSTGGQGG 146
           R  +D +G+E        PG G GFS D G  + F      R     F            
Sbjct: 63  RAIYDQYGEEGLTSQAPPPGAGGGFS-DGGASFRF----NGRSADDIF------------ 122

Query: 147 SQSFSFS--FGGPTGSNPFSSFGM-EDIFSN 168
           S+ F F+  FG   G+ P + F   ED+FS+
Sbjct: 123 SEFFGFTRPFGDSRGAGPSNGFRFAEDVFSS 136

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905464.19.8e-27686.70dnaJ protein ERDJ3A isoform X1 [Benincasa hispida][more]
XP_022935557.11.7e-26783.91dnaJ protein ERDJ3A [Cucurbita moschata] >KAG6580462.1 DnaJ protein ERDJ3A, part... [more]
TYK24944.16.4e-26784.60dnaJ protein ERDJ3A [Cucumis melo var. makuwa][more]
XP_023529005.19.2e-26683.39dnaJ protein ERDJ3A [Cucurbita pepo subsp. pepo][more]
XP_004137701.11.6e-26584.08dnaJ protein ERDJ3A isoform X1 [Cucumis sativus] >KGN58737.1 hypothetical protei... [more]
Match NameE-valueIdentityDescription
Q9SR968.0e-18058.66DnaJ protein ERDJ3A OS=Arabidopsis thaliana OX=3702 GN=ERDJ3A PE=1 SV=1[more]
Q10MW62.4e-16052.67DnaJ protein ERDJ3A OS=Oryza sativa subsp. japonica OX=39947 GN=ERDJ3A PE=1 SV=2[more]
B1HUD09.3e-1949.07Chaperone protein DnaJ OS=Lysinibacillus sphaericus (strain C3-41) OX=444177 GN=... [more]
Q8EUM42.7e-1842.38Chaperone protein DnaJ OS=Mycoplasma penetrans (strain HF-2) OX=272633 GN=dnaJ P... [more]
Q49Y211.3e-1745.21Chaperone protein DnaJ OS=Staphylococcus saprophyticus subsp. saprophyticus (str... [more]
Match NameE-valueIdentityDescription
A0A6J1F5W98.1e-26883.91dnaJ protein ERDJ3A OS=Cucurbita moschata OX=3662 GN=LOC111442396 PE=4 SV=1[more]
A0A5D3DMX53.1e-26784.60DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold352G... [more]
A0A5A7TMT47.6e-26684.46DnaJ protein ERDJ3A OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold236G... [more]
A0A0A0LDD27.6e-26684.08J domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G730990 PE=4 SV=1[more]
A0A1S3B5427.6e-26684.46dnaJ protein ERDJ3A OS=Cucumis melo OX=3656 GN=LOC103486271 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G08970.15.7e-18158.66DNAJ heat shock N-terminal domain-containing protein [more]
AT2G22360.11.2e-1653.85DNAJ heat shock family protein [more]
AT4G39960.13.2e-1436.93Molecular chaperone Hsp40/DnaJ family protein [more]
AT5G03160.14.1e-1441.13homolog of mamallian P58IPK [more]
AT3G08910.12.0e-1337.75DNAJ heat shock family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 62..82
NoneNo IPR availableCOILSCoilCoilcoord: 402..429
NoneNo IPR availableGENE3D3.40.30.10Glutaredoxincoord: 197..310
e-value: 2.4E-7
score: 32.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 423..442
NoneNo IPR availablePANTHERPTHR45184:SF1DNAJ PROTEIN ERDJ3Acoord: 8..573
NoneNo IPR availablePANTHERPTHR45184DNAJ PROTEIN ERDJ3Acoord: 8..573
IPR001623DnaJ domainPRINTSPR00625JDOMAINcoord: 48..63
score: 45.07
coord: 84..103
score: 30.66
coord: 64..84
score: 51.31
coord: 30..48
score: 50.49
IPR001623DnaJ domainSMARTSM00271dnaj_3coord: 27..84
e-value: 1.9E-30
score: 117.1
IPR001623DnaJ domainPFAMPF00226DnaJcoord: 28..89
e-value: 8.0E-26
score: 90.0
IPR001623DnaJ domainPROSITEPS50076DNAJ_2coord: 28..92
score: 22.0765
IPR001623DnaJ domainCDDcd06257DnaJcoord: 28..81
e-value: 7.98918E-25
score: 95.3049
IPR036869Chaperone J-domain superfamilyGENE3D1.10.287.110DnaJ domaincoord: 25..180
e-value: 1.3E-29
score: 104.9
IPR036869Chaperone J-domain superfamilySUPERFAMILY46565Chaperone J-domaincoord: 24..117
IPR018253DnaJ domain, conserved sitePROSITEPS00636DNAJ_1coord: 69..88
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 443..561
IPR036249Thioredoxin-like superfamilySUPERFAMILY52833Thioredoxin-likecoord: 194..310

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027288.1Lag0027288.1mRNA