Lag0027086 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0027086
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptioninteractor of constitutive active ROPs 2, chloroplastic
Locationchr10: 44938182 .. 44941699 (+)
RNA-Seq ExpressionLag0027086
SyntenyLag0027086
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGACACCGAAAGCGAAGTAAGATTATCGTTTAGTTTTTGAATTGCTTAGCTGTTTGCTTCCTCTGTCAGCTTAATCAAACGGTTTTCTTTTGTTGTATATTTTTGGCTCCTTCCAATCCAGAGCTGAACTTCCCACTTTTCATTCATCTGTTCTGCTTGTTTTTTTTTTTTTTACTGTAATCTGTATTATTTATGTTCTTTAAAGAACTAATATTGTCCAAGATGTAATCATGTAAGTCTAAAACTCTCTAGGAGGTGCAACATATGATATTTCAGCCTCTTGGTGCTCTAAATGCATTTAGCAATGCCAAATTGTCAATGTACTGAATGGTTTATTTGTTGGGTTAGATGGTAATTCTCAGTTTTGATTTTTCAAGTTGGTGATTGAAAAACGAGGGAGAGGTAAAGAAGAAGATAAAGAAGACTCCCATAGATAACTTAATTTAGTATATTGGATGTGCTTGAGGTATGTGATTGAGATTGAGAATTTATGTATCATTGCAGAACTGGAAATACTAATGGAATGTAGTTGACAAGGTAGTTTTTTTTGCACCATAGTTTGTAGGAGAGAGAGATTGTTAGACCTTATGAGTGTGGGTGATCTTGCTAATCTATTTAATAATAAAGCATAATAGCATGTATGATGTACCAAACGGAAACTAAACTCAACTTAATGGTGAAGATTGATATGAGATTGCTTTATCCTTAATTACAAGTCAAGCTATGCTAAACACGGAATACGCAGATGGTGAAGTACACTGTTTGTCTGTCTATGATATCTATTAAATCACCCATCAATCCAAAAGCTTAAACTTTTGGATTGTGTGGTGATTTAACATGGTATCAGAATAAGAGGTCCTATATTCGAACTTTTGTAAAGACATTTCATCCCCAATTAATATTGATTTCCACTTGTTAGGAAAAAATGAGGGGCATGTTAAACTATTGATATAATTTAAGTTATTATAACCCATCAGCTTAAGCTTTTGGATTGATTGGTGATTTAATGATATCTGTGTTTCACCGTTCTCCTCTCTCGGATGAAAGATCTCTTTTGGTTCCATGGCACCTAGTGTTGTAGCTAAGTAGATAACTGATAAGTTATACTATGTCCAGATCTTAAGTTGTTTGAATTGGATTGCAGAACTGGCTCCTCAGAAGTACCTCAAAGAAAATCCCCAAGGACACCTCGAACTGCTCGTCAACTTAAAACTCCAGGCTCAGATCCTGATTCCATTTCCACTTCTCCACTTGCTACTAGTAAGACACCTAAAGAGAGAAGTCCTAGAGTGGTGGCTGACCGCAAGTCACCACGATGTCTGGCCACTGAGGTACTCGTTTTACATTTTATGACTAACTATTGAGATTCATATTCTACTTACATTATACCATACCACTTTGATTTGTTAATGAGTTAAATTATAAATTTAGTCCTTTGAACATTTTAAGTCTGTGTAAGTCCTTGGACTTTTAAAAGTGTCTAATAGATAGTTAAAATTTTAATTTTGTGTCCAACAAGTCTCTGAACTTTAATTTTTTTAATAAGTTCATGAACTTTCAATTTTGAGTCTAATAGATCCTTAACTAGTCAAAATTTTTAAAAATTAAATTTGATTTTTGTCTAATAGAACTCTAAACTTTCAATTTTGTGACTAGTAGGTCTTGAACTTCAAAAAAGTCAAAAAAGTCAAGGACCTATTGGATACAAAATTGAAAGTATCTATTAGACAATTTGTAAAGTTTAGGGACCAAATAAACAAAACTTGAAAGTTATAAGACCTACTAAATACTTTTTAAGGTTTGGTGACCAAATAGACACAAATTAGAAAGTTCATGGACTAAACTTATAATTTAACATTCGTTAATATCAAGAACTTTTTTCTCATTTCAGAGTAAAGGGCCTAGCAAAGTGGCTGAATTGGAATCACAACTTTCTCAACTCCAGGAGGAACTCAAGAAGGCAAGTGACCAACTGAGCGCATCTGAATCACTAAAAAAGCAAGCCCAACAGGAGGCAGAAGAAGCAAAGAAACAGCTATCAGATATGTCTGCAAAGCTTGAAGAATCCCAACAGCAGGTGGTTGAGCTCTCTGCTTCGGAGGAAGATCGTGTTCGAGAACTGCACAAAATTTCTCAAGATCGCGATCGAGCTTGGCAGTCTGAGCTTGAGGCTGTACAGAAGCAGCACGCAATGGATGCTGCTGCATTGGCCTCTGGCATGAATGAAGTTCAGAGGCTCAAGGTCCAGCTGGAGATGGTGTCTGAATCCGAGGAAACCCGGAGTAAGCTTGCTGAGTCTGCTCAAGCTGAGATAGATCATTTAAGAACACAGCTCTCAGAGACTCTTTCCCTAGTTGAGAACCTGAAAAATGAGCTTAGTTACTGCAGAGAATCTGAAGCTCAAGCCCTTGAAGTTGCAAGGAAAAACCAAAATCAATTTGAAACAGCCAAGGCAGCTGTGGAAAAGCTCCAATCAGATGGAATCAAAGCCATTGAGGCTTACAGCTCTTTGTCATTAGAGTTGGAGCAATCAAAAGCTCAGATTCAATCATTGGAGGAACAGATCAGCAAGATTCAGCAAGGTCTGGTAGATTCTACAAGCAGTGCTGAGGAAAACGATGGGAAGGATGAAATCAATGAGATAAAAACTGAGCTTACCGCTATGAGATTGGAAGCAGATAAATCAAAATCTGCATTAGATGCAGCTGAGACGAGGTACGAGGAGGAATCCGTTCGTACAGCATTGCAAATTAGAATTGCTCATGAACTTGTGGAACAAATGAAAGTAGAGTCATGTAAGAAAGAGGAAGAATTGGATGCAGAACTCAAGGAGGCCAAAGCAAATATTGAACAACTGAGAGTAGACCTTAAGGAAAAAGAAACTCAATTGTGTAGCGTTGTGGAGGAAAACAAGGAGCTCAACTCAGAGATAAACAAAAGCATATCAGTCAATAGGGAGTCTGAACTAGCAATGGAGCTAAAGAAGTTAGAGGCTGACATGGCGGAGTTGAAGACCAAACTGTTGGATAAGGAGACAGAATTACAGAGTACATCCGAGGAAAACAAAGCACTTAGAAAGGAAATCGAAAAGGTAGAAATGGAAAGGGTTAAGATCAACGATGAAGCAGCCAGTTTGGCAGAAACAACCAAGGCTGCAGAACAAGAGGCACTGATGAAACTCGAGCAGGCATCGGAAGAGGCAGACAAAAGTAGCCGAAGAGCGGCACGAGTTGCCGAGCAGTTGGATGCTGCACAGGCTGCCAATTCCGAAATGGAGGCCGAGTTGAGGAGGTTGAAGGTGCAAGCAGACCAATGGAGGAAAGCAGCTGAGGCTGCTGCATCGATACTCTCAACAGGGAACAATGGGAAGTTTGTTGATAGAATAGTTTCTTTGGAGAACAATTATCCTCTAGGCTCGCCGTACTCGGAAGATCTCGACGACGAGTCGCCAAAGAAGAAGAATGGAAATGTGTTGAAGAAGATAGGAGTTTTGTGGAAGAAAAACCAGAAGTAA

mRNA sequence

ATGCAGACACCGAAAGCGAAAACTGGCTCCTCAGAAGTACCTCAAAGAAAATCCCCAAGGACACCTCGAACTGCTCGTCAACTTAAAACTCCAGGCTCAGATCCTGATTCCATTTCCACTTCTCCACTTGCTACTAGTAAGACACCTAAAGAGAGAAGTCCTAGAGTGGTGGCTGACCGCAAGTCACCACGATGTCTGGCCACTGAGAGTAAAGGGCCTAGCAAAGTGGCTGAATTGGAATCACAACTTTCTCAACTCCAGGAGGAACTCAAGAAGGCAAGTGACCAACTGAGCGCATCTGAATCACTAAAAAAGCAAGCCCAACAGGAGGCAGAAGAAGCAAAGAAACAGCTATCAGATATGTCTGCAAAGCTTGAAGAATCCCAACAGCAGGTGGTTGAGCTCTCTGCTTCGGAGGAAGATCGTGTTCGAGAACTGCACAAAATTTCTCAAGATCGCGATCGAGCTTGGCAGTCTGAGCTTGAGGCTGTACAGAAGCAGCACGCAATGGATGCTGCTGCATTGGCCTCTGGCATGAATGAAGTTCAGAGGCTCAAGGTCCAGCTGGAGATGGTGTCTGAATCCGAGGAAACCCGGAGTAAGCTTGCTGAGTCTGCTCAAGCTGAGATAGATCATTTAAGAACACAGCTCTCAGAGACTCTTTCCCTAGTTGAGAACCTGAAAAATGAGCTTAGTTACTGCAGAGAATCTGAAGCTCAAGCCCTTGAAGTTGCAAGGAAAAACCAAAATCAATTTGAAACAGCCAAGGCAGCTGTGGAAAAGCTCCAATCAGATGGAATCAAAGCCATTGAGGCTTACAGCTCTTTGTCATTAGAGTTGGAGCAATCAAAAGCTCAGATTCAATCATTGGAGGAACAGATCAGCAAGATTCAGCAAGGTCTGGTAGATTCTACAAGCAGTGCTGAGGAAAACGATGGGAAGGATGAAATCAATGAGATAAAAACTGAGCTTACCGCTATGAGATTGGAAGCAGATAAATCAAAATCTGCATTAGATGCAGCTGAGACGAGGTACGAGGAGGAATCCGTTCGTACAGCATTGCAAATTAGAATTGCTCATGAACTTGTGGAACAAATGAAAGTAGAGTCATGTAAGAAAGAGGAAGAATTGGATGCAGAACTCAAGGAGGCCAAAGCAAATATTGAACAACTGAGAGTAGACCTTAAGGAAAAAGAAACTCAATTGTGTAGCGTTGTGGAGGAAAACAAGGAGCTCAACTCAGAGATAAACAAAAGCATATCAGTCAATAGGGAGTCTGAACTAGCAATGGAGCTAAAGAAGTTAGAGGCTGACATGGCGGAGTTGAAGACCAAACTGTTGGATAAGGAGACAGAATTACAGAGTACATCCGAGGAAAACAAAGCACTTAGAAAGGAAATCGAAAAGGTAGAAATGGAAAGGGTTAAGATCAACGATGAAGCAGCCAGTTTGGCAGAAACAACCAAGGCTGCAGAACAAGAGGCACTGATGAAACTCGAGCAGGCATCGGAAGAGGCAGACAAAAGTAGCCGAAGAGCGGCACGAGTTGCCGAGCAGTTGGATGCTGCACAGGCTGCCAATTCCGAAATGGAGGCCGAGTTGAGGAGGTTGAAGGTGCAAGCAGACCAATGGAGGAAAGCAGCTGAGGCTGCTGCATCGATACTCTCAACAGGGAACAATGGGAAGTTTGTTGATAGAATAGTTTCTTTGGAGAACAATTATCCTCTAGGCTCGCCGTACTCGGAAGATCTCGACGACGAGTCGCCAAAGAAGAAGAATGGAAATGTGTTGAAGAAGATAGGAGTTTTGTGGAAGAAAAACCAGAAGTAA

Coding sequence (CDS)

ATGCAGACACCGAAAGCGAAAACTGGCTCCTCAGAAGTACCTCAAAGAAAATCCCCAAGGACACCTCGAACTGCTCGTCAACTTAAAACTCCAGGCTCAGATCCTGATTCCATTTCCACTTCTCCACTTGCTACTAGTAAGACACCTAAAGAGAGAAGTCCTAGAGTGGTGGCTGACCGCAAGTCACCACGATGTCTGGCCACTGAGAGTAAAGGGCCTAGCAAAGTGGCTGAATTGGAATCACAACTTTCTCAACTCCAGGAGGAACTCAAGAAGGCAAGTGACCAACTGAGCGCATCTGAATCACTAAAAAAGCAAGCCCAACAGGAGGCAGAAGAAGCAAAGAAACAGCTATCAGATATGTCTGCAAAGCTTGAAGAATCCCAACAGCAGGTGGTTGAGCTCTCTGCTTCGGAGGAAGATCGTGTTCGAGAACTGCACAAAATTTCTCAAGATCGCGATCGAGCTTGGCAGTCTGAGCTTGAGGCTGTACAGAAGCAGCACGCAATGGATGCTGCTGCATTGGCCTCTGGCATGAATGAAGTTCAGAGGCTCAAGGTCCAGCTGGAGATGGTGTCTGAATCCGAGGAAACCCGGAGTAAGCTTGCTGAGTCTGCTCAAGCTGAGATAGATCATTTAAGAACACAGCTCTCAGAGACTCTTTCCCTAGTTGAGAACCTGAAAAATGAGCTTAGTTACTGCAGAGAATCTGAAGCTCAAGCCCTTGAAGTTGCAAGGAAAAACCAAAATCAATTTGAAACAGCCAAGGCAGCTGTGGAAAAGCTCCAATCAGATGGAATCAAAGCCATTGAGGCTTACAGCTCTTTGTCATTAGAGTTGGAGCAATCAAAAGCTCAGATTCAATCATTGGAGGAACAGATCAGCAAGATTCAGCAAGGTCTGGTAGATTCTACAAGCAGTGCTGAGGAAAACGATGGGAAGGATGAAATCAATGAGATAAAAACTGAGCTTACCGCTATGAGATTGGAAGCAGATAAATCAAAATCTGCATTAGATGCAGCTGAGACGAGGTACGAGGAGGAATCCGTTCGTACAGCATTGCAAATTAGAATTGCTCATGAACTTGTGGAACAAATGAAAGTAGAGTCATGTAAGAAAGAGGAAGAATTGGATGCAGAACTCAAGGAGGCCAAAGCAAATATTGAACAACTGAGAGTAGACCTTAAGGAAAAAGAAACTCAATTGTGTAGCGTTGTGGAGGAAAACAAGGAGCTCAACTCAGAGATAAACAAAAGCATATCAGTCAATAGGGAGTCTGAACTAGCAATGGAGCTAAAGAAGTTAGAGGCTGACATGGCGGAGTTGAAGACCAAACTGTTGGATAAGGAGACAGAATTACAGAGTACATCCGAGGAAAACAAAGCACTTAGAAAGGAAATCGAAAAGGTAGAAATGGAAAGGGTTAAGATCAACGATGAAGCAGCCAGTTTGGCAGAAACAACCAAGGCTGCAGAACAAGAGGCACTGATGAAACTCGAGCAGGCATCGGAAGAGGCAGACAAAAGTAGCCGAAGAGCGGCACGAGTTGCCGAGCAGTTGGATGCTGCACAGGCTGCCAATTCCGAAATGGAGGCCGAGTTGAGGAGGTTGAAGGTGCAAGCAGACCAATGGAGGAAAGCAGCTGAGGCTGCTGCATCGATACTCTCAACAGGGAACAATGGGAAGTTTGTTGATAGAATAGTTTCTTTGGAGAACAATTATCCTCTAGGCTCGCCGTACTCGGAAGATCTCGACGACGAGTCGCCAAAGAAGAAGAATGGAAATGTGTTGAAGAAGATAGGAGTTTTGTGGAAGAAAAACCAGAAGTAA

Protein sequence

MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADRKSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSDMSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMNEVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQGLVDSTSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEINKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRRLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVLKKIGVLWKKNQK
Homology
BLAST of Lag0027086 vs. NCBI nr
Match: XP_022145717.1 (interactor of constitutive active ROPs 2, chloroplastic-like isoform X2 [Momordica charantia])

HSP 1 Score: 911.4 bits (2354), Expect = 4.2e-261
Identity = 531/619 (85.78%), Postives = 575/619 (92.89%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAKTGSSEVPQ+KSPRTPRTARQLK P S+P+SISTSPLA SKTPKERSPR V DR
Sbjct: 1   MQTPKAKTGSSEVPQKKSPRTPRTARQLKVPSSNPESISTSPLAASKTPKERSPR-VTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPRCLATESKGPSKVAELESQLS+LQEELKKASDQLSASES K++AQQEAEEAKKQLS+
Sbjct: 61  KSPRCLATESKGPSKVAELESQLSKLQEELKKASDQLSASESHKREAQQEAEEAKKQLSE 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLEESQQQV+ELSASEEDRV+ELHKISQDRD+AWQSELEAVQKQH MDAAALAS MN
Sbjct: 121 MSAKLEESQQQVLELSASEEDRVQELHKISQDRDQAWQSELEAVQKQHTMDAAALASAMN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLK QLEMVSESE TRS LAESAQ EI++LRTQLSET+ LVE LK+ELSYC+ESEAQ
Sbjct: 181 EVQRLKAQLEMVSESEATRSNLAESAQGEIEYLRTQLSETVFLVEKLKDELSYCKESEAQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
           ALEVARKNQNQFETAKAAVEKLQSDG+KAIE+Y+SLS+ELEQSKAQIQSLE QIS IQ+G
Sbjct: 241 ALEVARKNQNQFETAKAAVEKLQSDGMKAIESYNSLSVELEQSKAQIQSLEGQISMIQKG 300

Query: 301 LVD----------STSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESV 360
           LVD          +   AEEN G DEIN++KTELTAM++EADKSKSALDAAETRYEEE V
Sbjct: 301 LVDPMGNDLDPAVNAKPAEENQGSDEINQLKTELTAMKMEADKSKSALDAAETRYEEEYV 360

Query: 361 RTALQIRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENK 420
           RTALQIRIAHELVEQMKVESC+KE ELDAELK+A+ANIEQLRVDL EKETQLCSVVEENK
Sbjct: 361 RTALQIRIAHELVEQMKVESCRKEAELDAELKQARANIEQLRVDLNEKETQLCSVVEENK 420

Query: 421 ELNSEINKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKV 480
            LNSE +KS SV+RES+LA ELK+LEADM ELKT+LLDKETELQST+EEN+AL+KEIEK+
Sbjct: 421 ALNSETSKSTSVDRESKLATELKRLEADMMELKTRLLDKETELQSTAEENEALKKEIEKM 480

Query: 481 EMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEM 540
           EM+  KIND+A +LAETTKAAE+EALMKL  A+EEADKSSRRAARVAEQLDAAQAANSEM
Sbjct: 481 EMQTSKINDDAVALAETTKAAEEEALMKLGHATEEADKSSRRAARVAEQLDAAQAANSEM 540

Query: 541 EAELRRLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPK 600
           EAELRRLKVQADQWRKAAEAAA+ILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPK
Sbjct: 541 EAELRRLKVQADQWRKAAEAAAAILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPK 600

Query: 601 KKNGNVLKKIGVLWKKNQK 610
           KKNGN+LKKIGVLWKKNQK
Sbjct: 601 KKNGNMLKKIGVLWKKNQK 618

BLAST of Lag0027086 vs. NCBI nr
Match: XP_022145716.1 (interactor of constitutive active ROPs 2, chloroplastic-like isoform X1 [Momordica charantia])

HSP 1 Score: 899.4 bits (2323), Expect = 1.7e-257
Identity = 524/613 (85.48%), Postives = 569/613 (92.82%), Query Frame = 0

Query: 7   KTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADRKSPRCL 66
           +TGSSEVPQ+KSPRTPRTARQLK P S+P+SISTSPLA SKTPKERSPR V DRKSPRCL
Sbjct: 38  RTGSSEVPQKKSPRTPRTARQLKVPSSNPESISTSPLAASKTPKERSPR-VTDRKSPRCL 97

Query: 67  ATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSDMSAKLE 126
           ATESKGPSKVAELESQLS+LQEELKKASDQLSASES K++AQQEAEEAKKQLS+MSAKLE
Sbjct: 98  ATESKGPSKVAELESQLSKLQEELKKASDQLSASESHKREAQQEAEEAKKQLSEMSAKLE 157

Query: 127 ESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMNEVQRLK 186
           ESQQQV+ELSASEEDRV+ELHKISQDRD+AWQSELEAVQKQH MDAAALAS MNEVQRLK
Sbjct: 158 ESQQQVLELSASEEDRVQELHKISQDRDQAWQSELEAVQKQHTMDAAALASAMNEVQRLK 217

Query: 187 VQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQALEVAR 246
            QLEMVSESE TRS LAESAQ EI++LRTQLSET+ LVE LK+ELSYC+ESEAQALEVAR
Sbjct: 218 AQLEMVSESEATRSNLAESAQGEIEYLRTQLSETVFLVEKLKDELSYCKESEAQALEVAR 277

Query: 247 KNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQGLVD--- 306
           KNQNQFETAKAAVEKLQSDG+KAIE+Y+SLS+ELEQSKAQIQSLE QIS IQ+GLVD   
Sbjct: 278 KNQNQFETAKAAVEKLQSDGMKAIESYNSLSVELEQSKAQIQSLEGQISMIQKGLVDPMG 337

Query: 307 -------STSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQI 366
                  +   AEEN G DEIN++KTELTAM++EADKSKSALDAAETRYEEE VRTALQI
Sbjct: 338 NDLDPAVNAKPAEENQGSDEINQLKTELTAMKMEADKSKSALDAAETRYEEEYVRTALQI 397

Query: 367 RIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEI 426
           RIAHELVEQMKVESC+KE ELDAELK+A+ANIEQLRVDL EKETQLCSVVEENK LNSE 
Sbjct: 398 RIAHELVEQMKVESCRKEAELDAELKQARANIEQLRVDLNEKETQLCSVVEENKALNSET 457

Query: 427 NKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERVK 486
           +KS SV+RES+LA ELK+LEADM ELKT+LLDKETELQST+EEN+AL+KEIEK+EM+  K
Sbjct: 458 SKSTSVDRESKLATELKRLEADMMELKTRLLDKETELQSTAEENEALKKEIEKMEMQTSK 517

Query: 487 INDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRR 546
           IND+A +LAETTKAAE+EALMKL  A+EEADKSSRRAARVAEQLDAAQAANSEMEAELRR
Sbjct: 518 INDDAVALAETTKAAEEEALMKLGHATEEADKSSRRAARVAEQLDAAQAANSEMEAELRR 577

Query: 547 LKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNV 606
           LKVQADQWRKAAEAAA+ILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGN+
Sbjct: 578 LKVQADQWRKAAEAAAAILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNM 637

Query: 607 LKKIGVLWKKNQK 610
           LKKIGVLWKKNQK
Sbjct: 638 LKKIGVLWKKNQK 649

BLAST of Lag0027086 vs. NCBI nr
Match: XP_038905086.1 (interactor of constitutive active ROPs 3-like [Benincasa hispida] >XP_038905087.1 interactor of constitutive active ROPs 3-like [Benincasa hispida] >XP_038905088.1 interactor of constitutive active ROPs 3-like [Benincasa hispida] >XP_038905089.1 interactor of constitutive active ROPs 3-like [Benincasa hispida])

HSP 1 Score: 889.0 bits (2296), Expect = 2.3e-254
Identity = 535/662 (80.82%), Postives = 574/662 (86.71%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAKTGSSEVPQRKSPRTPRTARQLKTP SDPDS+STSPLA SKTPKERSPRVV DR
Sbjct: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPSSDPDSVSTSPLAASKTPKERSPRVVTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPRC+ATESKG SKVAEL SQ+SQLQEELKK SDQLSASES K+QAQQEAEEAKKQLSD
Sbjct: 61  KSPRCIATESKGHSKVAELGSQISQLQEELKKTSDQLSASESHKRQAQQEAEEAKKQLSD 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLEESQQQV+ELSASEEDRV+ELHKISQDRD+AWQSELEAV+KQH+MDAAALAS  N
Sbjct: 121 MSAKLEESQQQVLELSASEEDRVQELHKISQDRDKAWQSELEAVRKQHSMDAAALASATN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLKVQLEMVSESE TRSKLAES+Q EI+HLRTQLSETLSLVE LK+EL+YCRESEAQ
Sbjct: 181 EVQRLKVQLEMVSESEATRSKLAESSQTEIEHLRTQLSETLSLVEKLKDELTYCRESEAQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
           A EVARKN+NQFETAKAAVEKLQSDGIKAIEAY+SLS ELEQSKAQI+SLE QISKIQ+G
Sbjct: 241 AHEVARKNKNQFETAKAAVEKLQSDGIKAIEAYNSLSSELEQSKAQIESLEGQISKIQKG 300

Query: 301 LVDSTSSA---EENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIR 360
           LVDSTS+    EEN+GKDEI+ IKTELT+MRL+AD+SKSA  AAETRYEEE VR+ALQIR
Sbjct: 301 LVDSTSNGLYPEENNGKDEIDVIKTELTSMRLDADRSKSASAAAETRYEEEYVRSALQIR 360

Query: 361 IAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEIN 420
           IAHELVEQMKVESC+KE EL+AELKEA+AN+EQLRVDLKEKETQL SVVEENKELNSEI+
Sbjct: 361 IAHELVEQMKVESCQKEAELEAELKEARANLEQLRVDLKEKETQLYSVVEENKELNSEIS 420

Query: 421 KSISVNRESELAMELKKLEADM-------------------------------------- 480
           K I V+RESELAMELKKLEADM                                      
Sbjct: 421 KIIPVDRESELAMELKKLEADMEELKSRLLEKEKEVDGSTEENKALKIKIEKIEMERKSE 480

Query: 481 ------------AELKTKLLDKETELQSTSEENKALRKEIEKVEMERVKINDEAASLAET 540
                        ELKT+LL+KETELQST++EN AL+ EIEK++ME  KIND A SLAET
Sbjct: 481 LAMELKKFETDTVELKTRLLEKETELQSTTQENDALKMEIEKIKMETNKIND-AVSLAET 540

Query: 541 TKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRRLKVQADQWRKA 600
           TKAAEQEALMKLE A EEAD S+RRAARVAEQLDAAQAANSEMEAELRRLKVQADQWRKA
Sbjct: 541 TKAAEQEALMKLEHAREEADNSNRRAARVAEQLDAAQAANSEMEAELRRLKVQADQWRKA 600

Query: 601 AEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVLKKIGVLWKKN 610
           AEAAA+ILSTGNNGK VDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVLKKIGVLWKKN
Sbjct: 601 AEAAAAILSTGNNGKIVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVLKKIGVLWKKN 660

BLAST of Lag0027086 vs. NCBI nr
Match: KAG6580589.1 (Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 870.5 bits (2248), Expect = 8.3e-249
Identity = 513/620 (82.74%), Postives = 563/620 (90.81%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAK GSSEVPQRKSPRTPRTARQLKTP S PDS+STSPLA SK+P+ERSPRVV DR
Sbjct: 1   MQTPKAKVGSSEVPQRKSPRTPRTARQLKTPSSHPDSVSTSPLAASKSPRERSPRVVTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPR LATESK PSKVAELESQLSQLQEELKK SDQLSASES K+QAQQEAEEAKKQLSD
Sbjct: 61  KSPRSLATESKVPSKVAELESQLSQLQEELKKTSDQLSASESDKRQAQQEAEEAKKQLSD 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLEESQQQV+ELS SEEDRV+ELHKISQDRDRAWQSELEA+QKQH++DAA+LAS MN
Sbjct: 121 MSAKLEESQQQVLELSNSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLKVQLE  SESE T+SKLAESAQ EID LRTQLSETLSLVE LK+ELSYC+ESEAQ
Sbjct: 181 EVQRLKVQLETASESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQIS-KIQQ 300
           ALEVARKN NQFETAKAAVEKLQSDGIKAI+AY+SLSLELEQSKAQIQSLEEQIS KIQ+
Sbjct: 241 ALEVARKNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQK 300

Query: 301 GLVDS----------TSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEES 360
           GL+DS            +AE+NDGKD+I+++KTELTA+RLEADKSKSAL+AAE R+EEE 
Sbjct: 301 GLLDSEDNGLDPEVKAKAAEQNDGKDDISKVKTELTALRLEADKSKSALEAAEARFEEEY 360

Query: 361 VRTALQIRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEEN 420
           VRT LQIRIAHELVEQMKVESC+KE ELDAELKE +AN+EQLRVDLKEKETQLCS+VEEN
Sbjct: 361 VRTTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEEN 420

Query: 421 KELNSEINKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEK 480
           KELNSEI+K I V+RESELAMEL++LEADM ELK +LL+KET+LQ T+EEN AL+KEIE 
Sbjct: 421 KELNSEISKMIPVDRESELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIE- 480

Query: 481 VEMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSE 540
                 KIN+EA S+AETTKA E+EALMKLE A+EEADKSSRRAARVAEQLDAAQAA+SE
Sbjct: 481 ------KINEEAVSIAETTKATEKEALMKLEHATEEADKSSRRAARVAEQLDAAQAASSE 540

Query: 541 MEAELRRLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESP 600
           MEAELRRLKVQA+QWRKAAEAAA+ILSTGNNGK VD++V LENNYP+GSPYSEDL + SP
Sbjct: 541 MEAELRRLKVQAEQWRKAAEAAAAILSTGNNGKIVDKLVCLENNYPMGSPYSEDL-EASP 600

Query: 601 KKKNGNVLKKIGVLWKKNQK 610
           KKKNGNVLKKIGVLWKK QK
Sbjct: 601 KKKNGNVLKKIGVLWKKEQK 612

BLAST of Lag0027086 vs. NCBI nr
Match: XP_022983518.1 (interactor of constitutive active ROPs 2, chloroplastic [Cucurbita maxima])

HSP 1 Score: 867.8 bits (2241), Expect = 5.4e-248
Identity = 512/620 (82.58%), Postives = 560/620 (90.32%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAK GSSEVPQRKSPRTPRTARQL+TP S PDS+STSPLA SK+P+ERSPRVV DR
Sbjct: 1   MQTPKAKVGSSEVPQRKSPRTPRTARQLQTPSSHPDSVSTSPLAASKSPRERSPRVVTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPR LATESKGPSKVAELESQLSQLQEELKK SDQLSASES K+QA+QEAEEAKKQLSD
Sbjct: 61  KSPRSLATESKGPSKVAELESQLSQLQEELKKTSDQLSASESDKRQARQEAEEAKKQLSD 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLEESQQQV+ELS SEEDRV+ELHKISQDRDRAWQSELEA+QKQH++DAA+LAS MN
Sbjct: 121 MSAKLEESQQQVLELSTSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLKVQLE VSESE T+SKLAESAQ EID LRTQLSETLSLVE LK+ELSYC+ESEAQ
Sbjct: 181 EVQRLKVQLETVSESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQIS-KIQQ 300
           ALEV R N NQFETAKAAVEKLQSDGIKAI+AY+SLSLELEQSKAQIQSLEEQIS KIQ+
Sbjct: 241 ALEVVRTNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQK 300

Query: 301 GLVDS----------TSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEES 360
           GL+DS            +AEENDG DEI+E+KTELTAMRLEADKSKSAL+AAE R+EEE 
Sbjct: 301 GLLDSEDNDLDPDVNAKAAEENDGNDEIDEVKTELTAMRLEADKSKSALEAAEARFEEEY 360

Query: 361 VRTALQIRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEEN 420
           VRT LQIRIAHELVEQMKVESC+KE ELDAELKE +AN+EQLRVDLKEKETQLCS+VEEN
Sbjct: 361 VRTTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEEN 420

Query: 421 KELNSEINKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEK 480
           K LNSEI+K I ++RE ELAMEL++LEADM ELK +LL+KET+LQ T+EEN AL+KEIE 
Sbjct: 421 KVLNSEISKMIPIDREFELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIE- 480

Query: 481 VEMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSE 540
                 KIN+EA S+AETTKAAE+EALMKLE A+EEADKSSRRA RVAEQLDAAQAA+SE
Sbjct: 481 ------KINEEAVSIAETTKAAEKEALMKLEHATEEADKSSRRATRVAEQLDAAQAASSE 540

Query: 541 MEAELRRLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESP 600
           MEAELRRLKVQA+QWRKAAEAAA+ILSTGNNGK VD+IV LENNYP+GSPYSEDL + SP
Sbjct: 541 MEAELRRLKVQAEQWRKAAEAAAAILSTGNNGKIVDKIVCLENNYPMGSPYSEDL-EASP 600

Query: 601 KKKNGNVLKKIGVLWKKNQK 610
           KKKNGNVLKKIGVLWKK QK
Sbjct: 601 KKKNGNVLKKIGVLWKKEQK 612

BLAST of Lag0027086 vs. ExPASy Swiss-Prot
Match: Q9ZQC5 (Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICR2 PE=1 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 3.5e-117
Identity = 308/634 (48.58%), Postives = 409/634 (64.51%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSP-RTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVAD 60
           MQTPK + GS EVPQ+KSP  TP+TAR+LKT  SDP S   SP    +TPK +SP+VVAD
Sbjct: 1   MQTPKPRPGSLEVPQKKSPASTPKTARKLKTSESDPVS---SPNTKIRTPKTQSPKVVAD 60

Query: 61  RKSPRCLATE--SKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQ 120
           R+SPR    E   K   K  EL SQ+SQLQEELKKA +QLSASE+LKK+AQ +AEE K  
Sbjct: 61  RRSPRTPVNEIQKKRTGKTPELASQISQLQEELKKAKEQLSASEALKKEAQDQAEETK-- 120

Query: 121 LSDMSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALAS 180
                       QQ++E++ASE+ R+ EL K+SQ+RD+AWQSELEA+Q+QHAMD+AAL+S
Sbjct: 121 ------------QQLMEINASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAALSS 180

Query: 181 GMNEVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRES 240
            MNEVQ+LK QL   SESE             +++LR +L+ETLSLVE L+ EL   +E 
Sbjct: 181 TMNEVQKLKAQL---SESE------------NVENLRMELNETLSLVEKLRGELFDAKEG 240

Query: 241 EAQALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKI 300
           EAQA E+    + Q E A   +E L+SDG+K  EA +SL+ ELEQSK++++SLE+ + ++
Sbjct: 241 EAQAHEIVSGTEKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLVRQL 300

Query: 301 QQGLVDSTSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIR 360
           ++   +  +    N     + E+K E+   R E  + KSA++  E RY EE +++ LQIR
Sbjct: 301 EE---EDEARGNANGDSSSVEELKEEINVARQEISQLKSAVEVTERRYHEEYIQSTLQIR 360

Query: 361 IAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEIN 420
            A+E V+++K    ++E EL  ELK+ KA  + L   L +KE +L  +V+EN+ LNS+I 
Sbjct: 361 TAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLMDKEAKLRILVDENEILNSKIK 420

Query: 421 KSISV---------NRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIE 480
           +   V           E E   ELKKLE+D+ EL+  L+DKE ELQS   + ++LR E+E
Sbjct: 421 EKEEVYLNLENSLNQNEPEDTGELKKLESDVMELRANLMDKEMELQSVMSQYESLRSEME 480

Query: 481 KVEMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANS 540
            ++ E+ K  D              EAL KL   +EEADKS +RA    EQL AAQ  N+
Sbjct: 481 TMQSEKNKAID--------------EALAKLGSLTEEADKSGKRAENATEQLGAAQVTNT 540

Query: 541 EMEAELRRLKVQADQWRKAAEAAASILSTGN-----NGKFVDRIVSLENNYPLG------ 600
           E+EAELRRLKVQ DQWRKAAEAAA++LS GN     NGK+V+R  SLE+  PL       
Sbjct: 541 ELEAELRRLKVQCDQWRKAAEAAATMLSGGNNNNNSNGKYVERTGSLES--PLRRRNVNM 583

Query: 601 SPYSEDLDDE--SPKKKNGNVLKKIGVLWKKNQK 610
           SPY  + DDE  SPKKKNG++LKKIGVL KK+QK
Sbjct: 601 SPYMGETDDELSSPKKKNGSMLKKIGVLLKKSQK 583

BLAST of Lag0027086 vs. ExPASy Swiss-Prot
Match: Q9LSS5 (Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3 PE=1 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 2.9e-103
Identity = 274/614 (44.63%), Postives = 390/614 (63.52%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQT KA+ GS +VP++ S   PR AR LK    +P+S S+   AT++TPK++SP V+ +R
Sbjct: 1   MQTQKARNGSPDVPKKVS---PRAARPLKIAALEPESSSSPISATNRTPKDKSPNVL-NR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           +SPR   +E K PS++ ELE  +SQLQEELKKA DQ+S SE+ KKQA+QEAEE++KQL +
Sbjct: 61  RSPRSPVSEKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQE 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           +S+KLEESQ Q VE SA EE+  +    + Q   +    E  A     A + A LA+  +
Sbjct: 121 VSSKLEESQNQFVETSALEEETDKTGSLVFQSVSQECDWEFSAT----AGERAGLAAVAH 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           E+++LK+Q+EMV+ SE    K AE   +E+  LR  L +TL  VEN +N+L  C  SEA+
Sbjct: 181 EIRQLKLQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAE 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
              +A +   Q E AK AVE+L+SDG KA+E+Y  +++ELEQSK+++  LE  ++K+Q  
Sbjct: 241 TEALATETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVNKLQNN 300

Query: 301 LVDSTS-----SAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQ 360
             D  +        E+  + E NE+  E++++R E ++ ++AL+A++ + +E +V  + +
Sbjct: 301 PADLENHEILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSR 360

Query: 361 IRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSE 420
           +RI               + EL +ELK AK+ I++L+  L +KET+L  + EE    + +
Sbjct: 361 LRI---------------QAELQSELKIAKSEIDELKARLMDKETELQFISEERDNFSMK 420

Query: 421 INKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERV 480
           + K+    +E ++  ELKKL   +  LK  L+DKETELQ  S+EN+ L+ +I K E +  
Sbjct: 421 LMKN---QKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHKSETD-- 480

Query: 481 KINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELR 540
                            Q+A +KL  A EEADKSS++A RV EQL+A QA+NSEME ELR
Sbjct: 481 ----------------VQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELR 540

Query: 541 RLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGN 600
           +LKVQ++QWRKAAEAA ++LS GNNGKF       EN     SPYSED+DDE  KKKNGN
Sbjct: 541 KLKVQSNQWRKAAEAATAMLSAGNNGKFA------ENYNQTNSPYSEDIDDELTKKKNGN 564

Query: 601 VLKKIGVLWKKNQK 610
           VLKKIGVLWKK QK
Sbjct: 601 VLKKIGVLWKKPQK 564

BLAST of Lag0027086 vs. ExPASy Swiss-Prot
Match: Q8VYU8 (Interactor of constitutive active ROPs 5 OS=Arabidopsis thaliana OX=3702 GN=ICR5 PE=1 SV=2)

HSP 1 Score: 199.1 bits (505), Expect = 1.4e-49
Identity = 206/612 (33.66%), Postives = 286/612 (46.73%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSP-RTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVAD 60
           MQTPK++ GS E+PQ+KSP   P+  R+LK  G++ D        T    K + P+VVAD
Sbjct: 1   MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESDP------KTKTISKTQIPKVVAD 60

Query: 61  RKSPRCLATE--SKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQ 120
           R+S R    E   K   ++ ELES +SQLQEELKKA ++L+ SE+LK++AQ+EAE+AK Q
Sbjct: 61  RRSARIPLNEIQKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQ 120

Query: 121 LSDMSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALAS 180
           L D              ++ASE+ R+ EL K+SQ+RD+ WQSELEA+Q+QH MD+ AL+S
Sbjct: 121 LMD--------------INASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSS 180

Query: 181 GMNEVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRES 240
            +NEVQ+LK             SKL ES                                
Sbjct: 181 AINEVQKLK-------------SKLFESES------------------------------ 240

Query: 241 EAQALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKI 300
                                                    ELEQSK +++SLE+ + ++
Sbjct: 241 -----------------------------------------ELEQSKYEVRSLEKLVRQL 300

Query: 301 QQGLVDSTSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIR 360
           ++  V+S  S+       E+ E+K  +   R E  + KSA++AAETRY+EE +++ LQIR
Sbjct: 301 EEERVNSRDSSSSM----EVEELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTLQIR 360

Query: 361 IAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEIN 420
            A+E  E +K    ++E EL  EL   K  IE LR +L EK                   
Sbjct: 361 SAYEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKV------------------ 396

Query: 421 KSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERVKI 480
                 +E E   +LKKLE+D+ E++  L+DKE ELQ        LR  +EK        
Sbjct: 421 ------KEDESTGDLKKLESDLMEVRGSLMDKEMELQ-------ILRSAMEK-------- 396

Query: 481 NDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRRL 540
                               K+E A+ EA                       MEAEL+R+
Sbjct: 481 --------------------KVETANTEA-----------------------MEAELKRV 396

Query: 541 KVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVL 600
           K+Q +QWRKAAE AASIL   NN +  +R  S+E +                      +L
Sbjct: 541 KIQCEQWRKAAETAASIL---NNDE--ERTDSIETS---------------------KML 396

Query: 601 KKIGVLWKKNQK 610
           KK GVL KKN K
Sbjct: 601 KKFGVLLKKNHK 396

BLAST of Lag0027086 vs. ExPASy Swiss-Prot
Match: F4I8B9 (Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At1g65010 PE=1 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 6.8e-12
Identity = 150/551 (27.22%), Postives = 265/551 (48.09%), Query Frame = 0

Query: 5   KAKTGSSEVPQRK-SPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADRKSP 64
           + KTG  E P+ K SP  PR +   K   S  DS S SP+  ++   +RSP  V  + +P
Sbjct: 4   RTKTGLMETPRSKPSPPPPRLS---KLSASKSDSNSASPVPNTRLSLDRSPPTVNSKPTP 63

Query: 65  RCLATESKGPSKV-------AELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKK 124
               +    P KV        EL++QL+Q+QE+LKKA +Q+   +  K +A  + +E++K
Sbjct: 64  DRRPSRIPTPEKVHSRLVKGTELQTQLNQIQEDLKKADEQIELLKKDKAKAIDDLKESEK 123

Query: 125 QLSDMSAKLEE---SQQQVVELSASEEDRVRELHKIS----QDRDRAWQSELEAVQKQHA 184
            + + + KL+E   +Q++  E    E+ R  EL +      Q +D   ++ELE+++ QHA
Sbjct: 124 LVEEANEKLKEALAAQKRAEESFEVEKFRAVELEQAGLEAVQKKDVTSKNELESIRSQHA 183

Query: 185 MDAAALASGMNEVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETL-SLVENLK 244
           +D +AL S   E+QR+K +L M ++++      AE A  +I  +  + +E L S +  LK
Sbjct: 184 LDISALLSTTEELQRVKHELSMTADAKNKALSHAEEA-TKIAEIHAEKAEILASELGRLK 243

Query: 245 NELSYCRESEA-QALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQI 304
             L    E EA +  E+  K +++ E  +  +EK+           S L   L++ +  +
Sbjct: 244 ALLGSKEEKEAIEGNEIVSKLKSEIELLRGELEKV-----------SILESSLKEQEGLV 303

Query: 305 QSLEEQISKIQQGLVDSTSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEE 364
           + L+  +   +     + SS EE   K++++E++ E+     E+++SKS+  A+E+    
Sbjct: 304 EQLKVDLEAAKMAESCTNSSVEE--WKNKVHELEKEVE----ESNRSKSS--ASESM--- 363

Query: 365 ESVRTALQIRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVE 424
           ESV   L   + H L E     + +KE     +++  +  IE  R DL+E   Q+C   E
Sbjct: 364 ESVMKQL-AELNHVLHETKSDNAAQKE-----KIELLEKTIEAQRTDLEEYGRQVCIAKE 423

Query: 425 ENKELNSEINKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEI 484
           E  +L + +    S+  E E++           E KT+ LD E    S  +     R E+
Sbjct: 424 EASKLENLVE---SIKSELEISQ----------EEKTRALDNEKAATSNIQNLLDQRTEL 483

Query: 485 EKVEMERVKINDEAASL-AETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAA 538
             +E+ER K+ +E +    E+   A QEA  +  +A                Q+D+ + A
Sbjct: 484 -SIELERCKVEEEKSKKDMESLTLALQEASTESSEAKATLLVCQEELKNCESQVDSLKLA 508

BLAST of Lag0027086 vs. ExPASy Swiss-Prot
Match: F4JJP1 (WEB family protein At4g27595, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At4g27595 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.1e-05
Identity = 124/547 (22.67%), Postives = 257/547 (46.98%), Query Frame = 0

Query: 5   KAKTGSSEVPQRK-SPRTPRTARQ--LKTPGSDPDSISTSPLATSKTPKERSPRVVADRK 64
           + KTG  E P+ K SP TPR ++    K+ G+ P  + ++ L+  ++P+  + + V+DR+
Sbjct: 4   RTKTGLMETPRSKPSPPTPRVSKPTVTKSDGNSPSPVQSTRLSIDRSPQTVNSKPVSDRR 63

Query: 65  SPR--------------CLATESKGPSKVAELESQL-SQLQEELKKASDQLSASESLKKQ 124
           + R              C+A + K  S++ +    L  Q QE+L+KA++Q+   +  K +
Sbjct: 64  TARVPTPPEANYFLIIICMAFQ-KSQSRLGKGTGLLVQQTQEDLRKANEQIERLKKDKAK 123

Query: 125 AQQEAEEAKKQLSDMSAKLEE---SQQQVVELSASEEDRVRELHKIS----QDRDRAWQS 184
           A  + +E++K   + + KL E   +Q    + S  E+ R  EL +        ++ +W+ 
Sbjct: 124 ALDDLKESEKLTKEANEKLREALAAQHHAEKSSEIEKFRAVELEQAGIEAVHKKEVSWKK 183

Query: 185 ELEAVQKQHAMDAAALASGMNEVQRLKVQLEMVSESE-------ETRSKLAESAQAEIDH 244
           E+E+++ QHA+D +AL S   E+ R+K +L M ++++       E  +K+AE+   + + 
Sbjct: 184 EVESIRSQHALDISALLSTTEELHRIKQELAMTADAKNKALSHAEEATKIAENQAEKAEI 243

Query: 245 LRTQLSETLSLVENLKNELSYCRESEAQALEVARKNQNQFETAKAAVEKLQSDGIKAIEA 304
           L ++LS   +LV +  +E     E +    EV  K +++ E  +  +EK+         +
Sbjct: 244 LSSELSRLKALVGS--DEQKKSNEDD----EVVSKLKSEIEMLRGKLEKV---------S 303

Query: 305 YSSLSLELEQSKAQIQSLEEQISKIQQGLVDSTSSAEENDGKDEINEIKTELTAMRLEAD 364
               +L+ ++   ++  ++ Q +K+ +   ++ ++  +N+   ++ E K   T+     D
Sbjct: 304 ILENTLKDQEESIELLHVDLQAAKMVESYANNLAAEWKNEVDKQVEESKELKTSASESLD 363

Query: 365 KSKSALDAAETRYEEESVRTALQIRIAHELVEQMKVESCKKEEELD----------AELK 424
            +   L+       E  +  A       E VE +     ++E +L+           E  
Sbjct: 364 LAMKQLEENNHALHEAELGNA----TLKEKVESLVTTIGRQENDLEESQHQVCISKEETS 423

Query: 425 EAKANIEQLRVDLKEKETQLCSVVEENKELNSEINKSISVNRESELAMELKKLEADMAEL 484
           + +  +E ++ DL+  + +    +   K   S+I   +S   ++ELA EL+  + +  ++
Sbjct: 424 KLEKLVESIKSDLETTQGEKVRALLNEKTATSQIQNLLS--EKTELATELENCKKEEEKI 483

Query: 485 KTKLLDKETELQSTSEENKALRKEIE--KVEMERVKINDEAASLAETTKAAEQEALMKLE 508
           K  +     +LQ  S E K  ++++   + E+E   +  E+  LAE  K   ++    LE
Sbjct: 484 KKAMESLTLDLQEVSVEAKEAKEKLLTCQAELELCGVQIESLKLAE--KDTNEKHGKMLE 526

BLAST of Lag0027086 vs. ExPASy TrEMBL
Match: A0A6J1CXH6 (interactor of constitutive active ROPs 2, chloroplastic-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111015104 PE=3 SV=1)

HSP 1 Score: 911.4 bits (2354), Expect = 2.1e-261
Identity = 531/619 (85.78%), Postives = 575/619 (92.89%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAKTGSSEVPQ+KSPRTPRTARQLK P S+P+SISTSPLA SKTPKERSPR V DR
Sbjct: 1   MQTPKAKTGSSEVPQKKSPRTPRTARQLKVPSSNPESISTSPLAASKTPKERSPR-VTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPRCLATESKGPSKVAELESQLS+LQEELKKASDQLSASES K++AQQEAEEAKKQLS+
Sbjct: 61  KSPRCLATESKGPSKVAELESQLSKLQEELKKASDQLSASESHKREAQQEAEEAKKQLSE 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLEESQQQV+ELSASEEDRV+ELHKISQDRD+AWQSELEAVQKQH MDAAALAS MN
Sbjct: 121 MSAKLEESQQQVLELSASEEDRVQELHKISQDRDQAWQSELEAVQKQHTMDAAALASAMN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLK QLEMVSESE TRS LAESAQ EI++LRTQLSET+ LVE LK+ELSYC+ESEAQ
Sbjct: 181 EVQRLKAQLEMVSESEATRSNLAESAQGEIEYLRTQLSETVFLVEKLKDELSYCKESEAQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
           ALEVARKNQNQFETAKAAVEKLQSDG+KAIE+Y+SLS+ELEQSKAQIQSLE QIS IQ+G
Sbjct: 241 ALEVARKNQNQFETAKAAVEKLQSDGMKAIESYNSLSVELEQSKAQIQSLEGQISMIQKG 300

Query: 301 LVD----------STSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESV 360
           LVD          +   AEEN G DEIN++KTELTAM++EADKSKSALDAAETRYEEE V
Sbjct: 301 LVDPMGNDLDPAVNAKPAEENQGSDEINQLKTELTAMKMEADKSKSALDAAETRYEEEYV 360

Query: 361 RTALQIRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENK 420
           RTALQIRIAHELVEQMKVESC+KE ELDAELK+A+ANIEQLRVDL EKETQLCSVVEENK
Sbjct: 361 RTALQIRIAHELVEQMKVESCRKEAELDAELKQARANIEQLRVDLNEKETQLCSVVEENK 420

Query: 421 ELNSEINKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKV 480
            LNSE +KS SV+RES+LA ELK+LEADM ELKT+LLDKETELQST+EEN+AL+KEIEK+
Sbjct: 421 ALNSETSKSTSVDRESKLATELKRLEADMMELKTRLLDKETELQSTAEENEALKKEIEKM 480

Query: 481 EMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEM 540
           EM+  KIND+A +LAETTKAAE+EALMKL  A+EEADKSSRRAARVAEQLDAAQAANSEM
Sbjct: 481 EMQTSKINDDAVALAETTKAAEEEALMKLGHATEEADKSSRRAARVAEQLDAAQAANSEM 540

Query: 541 EAELRRLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPK 600
           EAELRRLKVQADQWRKAAEAAA+ILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPK
Sbjct: 541 EAELRRLKVQADQWRKAAEAAAAILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPK 600

Query: 601 KKNGNVLKKIGVLWKKNQK 610
           KKNGN+LKKIGVLWKKNQK
Sbjct: 601 KKNGNMLKKIGVLWKKNQK 618

BLAST of Lag0027086 vs. ExPASy TrEMBL
Match: A0A6J1CX56 (interactor of constitutive active ROPs 2, chloroplastic-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111015104 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 8.1e-258
Identity = 524/613 (85.48%), Postives = 569/613 (92.82%), Query Frame = 0

Query: 7   KTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADRKSPRCL 66
           +TGSSEVPQ+KSPRTPRTARQLK P S+P+SISTSPLA SKTPKERSPR V DRKSPRCL
Sbjct: 38  RTGSSEVPQKKSPRTPRTARQLKVPSSNPESISTSPLAASKTPKERSPR-VTDRKSPRCL 97

Query: 67  ATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSDMSAKLE 126
           ATESKGPSKVAELESQLS+LQEELKKASDQLSASES K++AQQEAEEAKKQLS+MSAKLE
Sbjct: 98  ATESKGPSKVAELESQLSKLQEELKKASDQLSASESHKREAQQEAEEAKKQLSEMSAKLE 157

Query: 127 ESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMNEVQRLK 186
           ESQQQV+ELSASEEDRV+ELHKISQDRD+AWQSELEAVQKQH MDAAALAS MNEVQRLK
Sbjct: 158 ESQQQVLELSASEEDRVQELHKISQDRDQAWQSELEAVQKQHTMDAAALASAMNEVQRLK 217

Query: 187 VQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQALEVAR 246
            QLEMVSESE TRS LAESAQ EI++LRTQLSET+ LVE LK+ELSYC+ESEAQALEVAR
Sbjct: 218 AQLEMVSESEATRSNLAESAQGEIEYLRTQLSETVFLVEKLKDELSYCKESEAQALEVAR 277

Query: 247 KNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQGLVD--- 306
           KNQNQFETAKAAVEKLQSDG+KAIE+Y+SLS+ELEQSKAQIQSLE QIS IQ+GLVD   
Sbjct: 278 KNQNQFETAKAAVEKLQSDGMKAIESYNSLSVELEQSKAQIQSLEGQISMIQKGLVDPMG 337

Query: 307 -------STSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQI 366
                  +   AEEN G DEIN++KTELTAM++EADKSKSALDAAETRYEEE VRTALQI
Sbjct: 338 NDLDPAVNAKPAEENQGSDEINQLKTELTAMKMEADKSKSALDAAETRYEEEYVRTALQI 397

Query: 367 RIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEI 426
           RIAHELVEQMKVESC+KE ELDAELK+A+ANIEQLRVDL EKETQLCSVVEENK LNSE 
Sbjct: 398 RIAHELVEQMKVESCRKEAELDAELKQARANIEQLRVDLNEKETQLCSVVEENKALNSET 457

Query: 427 NKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERVK 486
           +KS SV+RES+LA ELK+LEADM ELKT+LLDKETELQST+EEN+AL+KEIEK+EM+  K
Sbjct: 458 SKSTSVDRESKLATELKRLEADMMELKTRLLDKETELQSTAEENEALKKEIEKMEMQTSK 517

Query: 487 INDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRR 546
           IND+A +LAETTKAAE+EALMKL  A+EEADKSSRRAARVAEQLDAAQAANSEMEAELRR
Sbjct: 518 INDDAVALAETTKAAEEEALMKLGHATEEADKSSRRAARVAEQLDAAQAANSEMEAELRR 577

Query: 547 LKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNV 606
           LKVQADQWRKAAEAAA+ILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGN+
Sbjct: 578 LKVQADQWRKAAEAAAAILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNM 637

Query: 607 LKKIGVLWKKNQK 610
           LKKIGVLWKKNQK
Sbjct: 638 LKKIGVLWKKNQK 649

BLAST of Lag0027086 vs. ExPASy TrEMBL
Match: A0A0A0LDX4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736850 PE=3 SV=1)

HSP 1 Score: 882.1 bits (2278), Expect = 1.3e-252
Identity = 515/612 (84.15%), Postives = 569/612 (92.97%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAKTGSSEVPQRKSPRTPRTARQLKTP +DPDS+STSPLA SKTPKERSPRVV DR
Sbjct: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPSTDPDSVSTSPLAASKTPKERSPRVVTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPRCLATESKG SKVAEL  QLSQLQEELKK SD+LSASES KKQAQQEAEEAKKQLSD
Sbjct: 61  KSPRCLATESKGHSKVAELGLQLSQLQEELKKTSDRLSASESHKKQAQQEAEEAKKQLSD 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLE+SQQQV+ELSASEEDRV+ELHKISQDRDRAWQSELEAVQKQH+MDAAALAS +N
Sbjct: 121 MSAKLEQSQQQVLELSASEEDRVQELHKISQDRDRAWQSELEAVQKQHSMDAAALASAIN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLKVQLEMVS+SE  RSKLAES+Q E+D LRTQLSETLSLVE LK+ELSYCRESE Q
Sbjct: 181 EVQRLKVQLEMVSDSELNRSKLAESSQTEMDDLRTQLSETLSLVEKLKDELSYCRESETQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
           ALEVARK++NQFETAKAAVEKLQSD IKA+EAY+SLSLELEQSKA+I+SLE QIS+ Q+G
Sbjct: 241 ALEVARKSKNQFETAKAAVEKLQSDAIKAVEAYNSLSLELEQSKARIESLEGQISENQKG 300

Query: 301 LVDSTSS---AEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIR 360
           LVDSTS+    EEN+GKDEI+ IKTELT+MRLEAD+SKSAL AAETR+EEE VR+ALQIR
Sbjct: 301 LVDSTSNDLGPEENNGKDEIDVIKTELTSMRLEADRSKSALAAAETRHEEEYVRSALQIR 360

Query: 361 IAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEIN 420
           IAHELVEQMKVES +KE EL AELKEA+A++E L+VD+KEKETQLCSVVEENKELNS+++
Sbjct: 361 IAHELVEQMKVESRQKEAELKAELKEARADLEHLKVDIKEKETQLCSVVEENKELNSKMS 420

Query: 421 KSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERVKI 480
           + I V+R SELAMELKKLEADM ELK +LL+KETELQST++EN AL+ EIEK+++E  KI
Sbjct: 421 QIIPVDRGSELAMELKKLEADMGELKNRLLEKETELQSTTQENDALKMEIEKIKVETNKI 480

Query: 481 NDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRRL 540
           N+EA +LAETTKAAEQEALMKL+ A+EEAD S+RR ARVAEQLDAAQAANSEMEAELRRL
Sbjct: 481 NEEAVTLAETTKAAEQEALMKLKHATEEADNSNRRVARVAEQLDAAQAANSEMEAELRRL 540

Query: 541 KVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVL 600
           KVQADQWRKAAEAAA+ILSTGNNGK VDRIVSL+NNYPLGSPYSEDLDDESPKKKNGN+L
Sbjct: 541 KVQADQWRKAAEAAAAILSTGNNGKIVDRIVSLDNNYPLGSPYSEDLDDESPKKKNGNML 600

Query: 601 KKIGVLWKKNQK 610
           KKIGVLWKKNQK
Sbjct: 601 KKIGVLWKKNQK 612

BLAST of Lag0027086 vs. ExPASy TrEMBL
Match: A0A6J1J7Z2 (interactor of constitutive active ROPs 2, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111482097 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.6e-248
Identity = 512/620 (82.58%), Postives = 560/620 (90.32%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAK GSSEVPQRKSPRTPRTARQL+TP S PDS+STSPLA SK+P+ERSPRVV DR
Sbjct: 1   MQTPKAKVGSSEVPQRKSPRTPRTARQLQTPSSHPDSVSTSPLAASKSPRERSPRVVTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPR LATESKGPSKVAELESQLSQLQEELKK SDQLSASES K+QA+QEAEEAKKQLSD
Sbjct: 61  KSPRSLATESKGPSKVAELESQLSQLQEELKKTSDQLSASESDKRQARQEAEEAKKQLSD 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLEESQQQV+ELS SEEDRV+ELHKISQDRDRAWQSELEA+QKQH++DAA+LAS MN
Sbjct: 121 MSAKLEESQQQVLELSTSEEDRVQELHKISQDRDRAWQSELEAIQKQHSIDAASLASAMN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLKVQLE VSESE T+SKLAESAQ EID LRTQLSETLSLVE LK+ELSYC+ESEAQ
Sbjct: 181 EVQRLKVQLETVSESEATQSKLAESAQTEIDCLRTQLSETLSLVEKLKDELSYCKESEAQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQIS-KIQQ 300
           ALEV R N NQFETAKAAVEKLQSDGIKAI+AY+SLSLELEQSKAQIQSLEEQIS KIQ+
Sbjct: 241 ALEVVRTNHNQFETAKAAVEKLQSDGIKAIDAYNSLSLELEQSKAQIQSLEEQISKKIQK 300

Query: 301 GLVDS----------TSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEES 360
           GL+DS            +AEENDG DEI+E+KTELTAMRLEADKSKSAL+AAE R+EEE 
Sbjct: 301 GLLDSEDNDLDPDVNAKAAEENDGNDEIDEVKTELTAMRLEADKSKSALEAAEARFEEEY 360

Query: 361 VRTALQIRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEEN 420
           VRT LQIRIAHELVEQMKVESC+KE ELDAELKE +AN+EQLRVDLKEKETQLCS+VEEN
Sbjct: 361 VRTTLQIRIAHELVEQMKVESCQKEAELDAELKETRANLEQLRVDLKEKETQLCSIVEEN 420

Query: 421 KELNSEINKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEK 480
           K LNSEI+K I ++RE ELAMEL++LEADM ELK +LL+KET+LQ T+EEN AL+KEIE 
Sbjct: 421 KVLNSEISKMIPIDREFELAMELQRLEADMVELKNRLLEKETQLQGTTEENDALKKEIE- 480

Query: 481 VEMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSE 540
                 KIN+EA S+AETTKAAE+EALMKLE A+EEADKSSRRA RVAEQLDAAQAA+SE
Sbjct: 481 ------KINEEAVSIAETTKAAEKEALMKLEHATEEADKSSRRATRVAEQLDAAQAASSE 540

Query: 541 MEAELRRLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESP 600
           MEAELRRLKVQA+QWRKAAEAAA+ILSTGNNGK VD+IV LENNYP+GSPYSEDL + SP
Sbjct: 541 MEAELRRLKVQAEQWRKAAEAAAAILSTGNNGKIVDKIVCLENNYPMGSPYSEDL-EASP 600

Query: 601 KKKNGNVLKKIGVLWKKNQK 610
           KKKNGNVLKKIGVLWKK QK
Sbjct: 601 KKKNGNVLKKIGVLWKKEQK 612

BLAST of Lag0027086 vs. ExPASy TrEMBL
Match: A0A1S3B5P5 (LOW QUALITY PROTEIN: interactor of constitutive active ROPs 3-like OS=Cucumis melo OX=3656 GN=LOC103486455 PE=3 SV=1)

HSP 1 Score: 867.8 bits (2241), Expect = 2.6e-248
Identity = 519/663 (78.28%), Postives = 569/663 (85.82%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQTPKAKTGSSEVPQRKSPRTPRTARQLKTP SDPDS+STSPLA SKTPKERSPRVV DR
Sbjct: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPSSDPDSVSTSPLAASKTPKERSPRVVTDR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           KSPRCLATESKG SKVAEL  QLSQLQEELKK SD+LS SES K+QAQQEAEEAKKQLSD
Sbjct: 61  KSPRCLATESKGHSKVAELGLQLSQLQEELKKTSDRLSTSESHKRQAQQEAEEAKKQLSD 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           MSAKLE+SQQQV+ELSASEEDRV+ELH+ISQDRDRAWQSELEAVQKQH+MDAAALAS +N
Sbjct: 121 MSAKLEQSQQQVLELSASEEDRVQELHRISQDRDRAWQSELEAVQKQHSMDAAALASAIN 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           EVQRLKVQLEMVSESE TRSKLAES+Q EIDHLRT+LSETLSLVE LK ELSYCRESE Q
Sbjct: 181 EVQRLKVQLEMVSESELTRSKLAESSQTEIDHLRTKLSETLSLVEKLKEELSYCRESETQ 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
           ALEVARK + QFETAKAAVEKLQSD IKA+EAY+SLSLELEQSKAQI+SLE QI + Q+G
Sbjct: 241 ALEVARKTKXQFETAKAAVEKLQSDAIKAVEAYNSLSLELEQSKAQIESLEGQIRENQKG 300

Query: 301 LVDSTSSA----EENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQI 360
           LVDSTS+     +EN+GKDEIN IKTELT+MRLEAD+SKSAL AAETRYEEE VR+ALQI
Sbjct: 301 LVDSTSNGLVGPQENNGKDEINLIKTELTSMRLEADRSKSALAAAETRYEEEYVRSALQI 360

Query: 361 RIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEI 420
           RIAHELVEQMKVES +KE EL AEL+EA+AN++QL+VDLKEKETQLCSVVEENKELNSE+
Sbjct: 361 RIAHELVEQMKVESRQKEAELKAELEEARANLDQLKVDLKEKETQLCSVVEENKELNSEV 420

Query: 421 NKSISVNRESELAMELKKLEADM------------------------------------- 480
           ++ I V+R SELAMELKKLEADM                                     
Sbjct: 421 SRIIPVDRGSELAMELKKLEADMGELKSRLLEKETELHSTMVENEALKKKIEKIDMERKS 480

Query: 481 -------------AELKTKLLDKETELQSTSEENKALRKEIEKVEMERVKINDEAASLAE 540
                        AELKT+LL+KETELQST++EN AL+ EIEK+++E  KIN+EA ++AE
Sbjct: 481 ELAVELKKFETDTAELKTRLLEKETELQSTTQENDALKMEIEKIKIETNKINEEAVTMAE 540

Query: 541 TTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRRLKVQADQWRK 600
           TTKAAEQEALMKL+ A+EEAD S+RR ARVAEQLDAAQAANSEMEAELRRLKVQADQWRK
Sbjct: 541 TTKAAEQEALMKLKHATEEADNSNRRVARVAEQLDAAQAANSEMEAELRRLKVQADQWRK 600

Query: 601 AAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVLKKIGVLWKK 610
           AAEAAA+ILSTGNNGK VDRIVSL+NNYPLGSPYSEDLDDESPKKKNGN+LKKIGVLWKK
Sbjct: 601 AAEAAAAILSTGNNGKIVDRIVSLDNNYPLGSPYSEDLDDESPKKKNGNMLKKIGVLWKK 660

BLAST of Lag0027086 vs. TAIR 10
Match: AT2G37080.1 (ROP interactive partner 3 )

HSP 1 Score: 423.7 bits (1088), Expect = 2.5e-118
Identity = 308/634 (48.58%), Postives = 409/634 (64.51%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSP-RTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVAD 60
           MQTPK + GS EVPQ+KSP  TP+TAR+LKT  SDP S   SP    +TPK +SP+VVAD
Sbjct: 1   MQTPKPRPGSLEVPQKKSPASTPKTARKLKTSESDPVS---SPNTKIRTPKTQSPKVVAD 60

Query: 61  RKSPRCLATE--SKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQ 120
           R+SPR    E   K   K  EL SQ+SQLQEELKKA +QLSASE+LKK+AQ +AEE K  
Sbjct: 61  RRSPRTPVNEIQKKRTGKTPELASQISQLQEELKKAKEQLSASEALKKEAQDQAEETK-- 120

Query: 121 LSDMSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALAS 180
                       QQ++E++ASE+ R+ EL K+SQ+RD+AWQSELEA+Q+QHAMD+AAL+S
Sbjct: 121 ------------QQLMEINASEDSRIDELRKLSQERDKAWQSELEAMQRQHAMDSAALSS 180

Query: 181 GMNEVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRES 240
            MNEVQ+LK QL   SESE             +++LR +L+ETLSLVE L+ EL   +E 
Sbjct: 181 TMNEVQKLKAQL---SESE------------NVENLRMELNETLSLVEKLRGELFDAKEG 240

Query: 241 EAQALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKI 300
           EAQA E+    + Q E A   +E L+SDG+K  EA +SL+ ELEQSK++++SLE+ + ++
Sbjct: 241 EAQAHEIVSGTEKQLEIANLTLEMLRSDGMKMSEACNSLTTELEQSKSEVRSLEQLVRQL 300

Query: 301 QQGLVDSTSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIR 360
           ++   +  +    N     + E+K E+   R E  + KSA++  E RY EE +++ LQIR
Sbjct: 301 EE---EDEARGNANGDSSSVEELKEEINVARQEISQLKSAVEVTERRYHEEYIQSTLQIR 360

Query: 361 IAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEIN 420
            A+E V+++K    ++E EL  ELK+ KA  + L   L +KE +L  +V+EN+ LNS+I 
Sbjct: 361 TAYEQVDEVKSGYAQREAELGEELKKTKAERDSLHERLMDKEAKLRILVDENEILNSKIK 420

Query: 421 KSISV---------NRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIE 480
           +   V           E E   ELKKLE+D+ EL+  L+DKE ELQS   + ++LR E+E
Sbjct: 421 EKEEVYLNLENSLNQNEPEDTGELKKLESDVMELRANLMDKEMELQSVMSQYESLRSEME 480

Query: 481 KVEMERVKINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANS 540
            ++ E+ K  D              EAL KL   +EEADKS +RA    EQL AAQ  N+
Sbjct: 481 TMQSEKNKAID--------------EALAKLGSLTEEADKSGKRAENATEQLGAAQVTNT 540

Query: 541 EMEAELRRLKVQADQWRKAAEAAASILSTGN-----NGKFVDRIVSLENNYPLG------ 600
           E+EAELRRLKVQ DQWRKAAEAAA++LS GN     NGK+V+R  SLE+  PL       
Sbjct: 541 ELEAELRRLKVQCDQWRKAAEAAATMLSGGNNNNNSNGKYVERTGSLES--PLRRRNVNM 583

Query: 601 SPYSEDLDDE--SPKKKNGNVLKKIGVLWKKNQK 610
           SPY  + DDE  SPKKKNG++LKKIGVL KK+QK
Sbjct: 601 SPYMGETDDELSSPKKKNGSMLKKIGVLLKKSQK 583

BLAST of Lag0027086 vs. TAIR 10
Match: AT5G60210.1 (ROP interactive partner 5 )

HSP 1 Score: 377.5 bits (968), Expect = 2.1e-104
Identity = 274/614 (44.63%), Postives = 390/614 (63.52%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQT KA+ GS +VP++ S   PR AR LK    +P+S S+   AT++TPK++SP V+ +R
Sbjct: 1   MQTQKARNGSPDVPKKVS---PRAARPLKIAALEPESSSSPISATNRTPKDKSPNVL-NR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           +SPR   +E K PS++ ELE  +SQLQEELKKA DQ+S SE+ KKQA+QEAEE++KQL +
Sbjct: 61  RSPRSPVSEKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQE 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           +S+KLEESQ Q VE SA EE+  +    + Q   +    E  A     A + A LA+  +
Sbjct: 121 VSSKLEESQNQFVETSALEEETDKTGSLVFQSVSQECDWEFSAT----AGERAGLAAVAH 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           E+++LK+Q+EMV+ SE    K AE   +E+  LR  L +TL  VEN +N+L  C  SEA+
Sbjct: 181 EIRQLKLQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAE 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
              +A +   Q E AK AVE+L+SDG KA+E+Y  +++ELEQSK+++  LE  ++K+Q  
Sbjct: 241 TEALATETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVNKLQNN 300

Query: 301 LVDSTS-----SAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQ 360
             D  +        E+  + E NE+  E++++R E ++ ++AL+A++ + +E +V  + +
Sbjct: 301 PADLENHEILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSR 360

Query: 361 IRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSE 420
           +RI               + EL +ELK AK+ I++L+  L +KET+L  + EE    + +
Sbjct: 361 LRI---------------QAELQSELKIAKSEIDELKARLMDKETELQFISEERDNFSMK 420

Query: 421 INKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERV 480
           + K+    +E ++  ELKKL   +  LK  L+DKETELQ  S+EN+ L+ +I K E +  
Sbjct: 421 LMKN---QKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHKSETD-- 480

Query: 481 KINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELR 540
                            Q+A +KL  A EEADKSS++A RV EQL+A QA+NSEME ELR
Sbjct: 481 ----------------VQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELR 540

Query: 541 RLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGN 600
           +LKVQ++QWRKAAEAA ++LS GNNGKF       EN     SPYSED+DDE  KKKNGN
Sbjct: 541 KLKVQSNQWRKAAEAATAMLSAGNNGKFA------ENYNQTNSPYSEDIDDELTKKKNGN 564

Query: 601 VLKKIGVLWKKNQK 610
           VLKKIGVLWKK QK
Sbjct: 601 VLKKIGVLWKKPQK 564

BLAST of Lag0027086 vs. TAIR 10
Match: AT5G60210.2 (ROP interactive partner 5 )

HSP 1 Score: 377.5 bits (968), Expect = 2.1e-104
Identity = 274/614 (44.63%), Postives = 390/614 (63.52%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSPRTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVADR 60
           MQT KA+ GS +VP++ S   PR AR LK    +P+S S+   AT++TPK++SP V+ +R
Sbjct: 1   MQTQKARNGSPDVPKKVS---PRAARPLKIAALEPESSSSPISATNRTPKDKSPNVL-NR 60

Query: 61  KSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLSD 120
           +SPR   +E K PS++ ELE  +SQLQEELKKA DQ+S SE+ KKQA+QEAEE++KQL +
Sbjct: 61  RSPRSPVSEKKRPSRITELELLVSQLQEELKKAKDQISVSETSKKQAEQEAEESRKQLQE 120

Query: 121 MSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGMN 180
           +S+KLEESQ Q VE SA EE+  +    + Q   +    E  A     A + A LA+  +
Sbjct: 121 VSSKLEESQNQFVETSALEEETDKTGSLVFQSVSQECDWEFSAT----AGERAGLAAVAH 180

Query: 181 EVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEAQ 240
           E+++LK+Q+EMV+ SE    K AE   +E+  LR  L +TL  VEN +N+L  C  SEA+
Sbjct: 181 EIRQLKLQIEMVASSEAGHVKQAELYNSEVQLLRGNLMDTLFHVENFRNQLKDCEISEAE 240

Query: 241 ALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQG 300
              +A +   Q E AK AVE+L+SDG KA+E+Y  +++ELEQSK+++  LE  ++K+Q  
Sbjct: 241 TEALATETLRQLENAKKAVEELKSDGTKAVESYKKMAVELEQSKSRMVWLEALVNKLQNN 300

Query: 301 LVDSTS-----SAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQ 360
             D  +        E+  + E NE+  E++++R E ++ ++AL+A++ + +E +V  + +
Sbjct: 301 PADLENHEILLKDYESLRRGESNEMDEEVSSLRCEVERLRAALEASDKKDQEGNVEASSR 360

Query: 361 IRIAHELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSE 420
           +RI               + EL +ELK AK+ I++L+  L +KET+L  + EE    + +
Sbjct: 361 LRI---------------QAELQSELKIAKSEIDELKARLMDKETELQFISEERDNFSMK 420

Query: 421 INKSISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERV 480
           + K+    +E ++  ELKKL   +  LK  L+DKETELQ  S+EN+ L+ +I K E +  
Sbjct: 421 LMKN---QKEIDVEAELKKLREAIENLKADLMDKETELQIVSDENETLKSDIHKSETD-- 480

Query: 481 KINDEAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELR 540
                            Q+A +KL  A EEADKSS++A RV EQL+A QA+NSEME ELR
Sbjct: 481 ----------------VQDAFLKLGIAMEEADKSSKKAVRVTEQLEATQASNSEMETELR 540

Query: 541 RLKVQADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGN 600
           +LKVQ++QWRKAAEAA ++LS GNNGKF       EN     SPYSED+DDE  KKKNGN
Sbjct: 541 KLKVQSNQWRKAAEAATAMLSAGNNGKFA------ENYNQTNSPYSEDIDDELTKKKNGN 564

Query: 601 VLKKIGVLWKKNQK 610
           VLKKIGVLWKK QK
Sbjct: 601 VLKKIGVLWKKPQK 564

BLAST of Lag0027086 vs. TAIR 10
Match: AT3G53350.2 (ROP interactive partner 4 )

HSP 1 Score: 204.1 bits (518), Expect = 3.1e-52
Identity = 206/610 (33.77%), Postives = 286/610 (46.89%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSP-RTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVAD 60
           MQTPK++ GS E+PQ+KSP   P+  R+LK  G++ D        T    K + P+VVAD
Sbjct: 1   MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESDP------KTKTISKTQIPKVVAD 60

Query: 61  RKSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLS 120
           R+S R    E K   ++ ELES +SQLQEELKKA ++L+ SE+LK++AQ+EAE+AK QL 
Sbjct: 61  RRSARIPLNEKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQLM 120

Query: 121 DMSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGM 180
           D              ++ASE+ R+ EL K+SQ+RD+ WQSELEA+Q+QH MD+ AL+S +
Sbjct: 121 D--------------INASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSAI 180

Query: 181 NEVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEA 240
           NEVQ+LK             SKL ES                                  
Sbjct: 181 NEVQKLK-------------SKLFESES-------------------------------- 240

Query: 241 QALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQ 300
                                                  ELEQSK +++SLE+ + ++++
Sbjct: 241 ---------------------------------------ELEQSKYEVRSLEKLVRQLEE 300

Query: 301 GLVDSTSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIRIA 360
             V+S  S+       E+ E+K  +   R E  + KSA++AAETRY+EE +++ LQIR A
Sbjct: 301 ERVNSRDSSSSM----EVEELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTLQIRSA 360

Query: 361 HELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEINKS 420
           +E  E +K    ++E EL  EL   K  IE LR +L EK                     
Sbjct: 361 YEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKV-------------------- 394

Query: 421 ISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERVKIND 480
               +E E   +LKKLE+D+ E++  L+DKE ELQ        LR  +EK          
Sbjct: 421 ----KEDESTGDLKKLESDLMEVRGSLMDKEMELQ-------ILRSAMEK---------- 394

Query: 481 EAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRRLKV 540
                             K+E A+ EA                       MEAEL+R+K+
Sbjct: 481 ------------------KVETANTEA-----------------------MEAELKRVKI 394

Query: 541 QADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVLKK 600
           Q +QWRKAAE AASIL   NN +  +R  S+E +                      +LKK
Sbjct: 541 QCEQWRKAAETAASIL---NNDE--ERTDSIETS---------------------KMLKK 394

Query: 601 IGVLWKKNQK 610
            GVL KKN K
Sbjct: 601 FGVLLKKNHK 394

BLAST of Lag0027086 vs. TAIR 10
Match: AT3G53350.3 (ROP interactive partner 4 )

HSP 1 Score: 204.1 bits (518), Expect = 3.1e-52
Identity = 206/610 (33.77%), Postives = 286/610 (46.89%), Query Frame = 0

Query: 1   MQTPKAKTGSSEVPQRKSP-RTPRTARQLKTPGSDPDSISTSPLATSKTPKERSPRVVAD 60
           MQTPK++ GS E+PQ+KSP   P+  R+LK  G++ D        T    K + P+VVAD
Sbjct: 1   MQTPKSRPGSLELPQKKSPLPAPKVVRRLKPSGAESDP------KTKTISKTQIPKVVAD 60

Query: 61  RKSPRCLATESKGPSKVAELESQLSQLQEELKKASDQLSASESLKKQAQQEAEEAKKQLS 120
           R+S R    E K   ++ ELES +SQLQEELKKA ++L+ SE+LK++AQ+EAE+AK QL 
Sbjct: 61  RRSARIPLNEKKRTGRIPELESTISQLQEELKKAKEELNRSEALKREAQEEAEDAKHQLM 120

Query: 121 DMSAKLEESQQQVVELSASEEDRVRELHKISQDRDRAWQSELEAVQKQHAMDAAALASGM 180
           D              ++ASE+ R+ EL K+SQ+RD+ WQSELEA+Q+QH MD+ AL+S +
Sbjct: 121 D--------------INASEDSRIEELRKLSQERDKTWQSELEAMQRQHGMDSTALSSAI 180

Query: 181 NEVQRLKVQLEMVSESEETRSKLAESAQAEIDHLRTQLSETLSLVENLKNELSYCRESEA 240
           NEVQ+LK             SKL ES                                  
Sbjct: 181 NEVQKLK-------------SKLFESES-------------------------------- 240

Query: 241 QALEVARKNQNQFETAKAAVEKLQSDGIKAIEAYSSLSLELEQSKAQIQSLEEQISKIQQ 300
                                                  ELEQSK +++SLE+ + ++++
Sbjct: 241 ---------------------------------------ELEQSKYEVRSLEKLVRQLEE 300

Query: 301 GLVDSTSSAEENDGKDEINEIKTELTAMRLEADKSKSALDAAETRYEEESVRTALQIRIA 360
             V+S  S+       E+ E+K  +   R E  + KSA++AAETRY+EE +++ LQIR A
Sbjct: 301 ERVNSRDSSSSM----EVEELKEAMNLSRQEITQLKSAVEAAETRYQEEYIQSTLQIRSA 360

Query: 361 HELVEQMKVESCKKEEELDAELKEAKANIEQLRVDLKEKETQLCSVVEENKELNSEINKS 420
           +E  E +K    ++E EL  EL   K  IE LR +L EK                     
Sbjct: 361 YEQTEAVKSRYSQREAELTEELNRTKDEIEGLRKELMEKV-------------------- 394

Query: 421 ISVNRESELAMELKKLEADMAELKTKLLDKETELQSTSEENKALRKEIEKVEMERVKIND 480
               +E E   +LKKLE+D+ E++  L+DKE ELQ        LR  +EK          
Sbjct: 421 ----KEDESTGDLKKLESDLMEVRGSLMDKEMELQ-------ILRSAMEK---------- 394

Query: 481 EAASLAETTKAAEQEALMKLEQASEEADKSSRRAARVAEQLDAAQAANSEMEAELRRLKV 540
                             K+E A+ EA                       MEAEL+R+K+
Sbjct: 481 ------------------KVETANTEA-----------------------MEAELKRVKI 394

Query: 541 QADQWRKAAEAAASILSTGNNGKFVDRIVSLENNYPLGSPYSEDLDDESPKKKNGNVLKK 600
           Q +QWRKAAE AASIL   NN +  +R  S+E +                      +LKK
Sbjct: 541 QCEQWRKAAETAASIL---NNDE--ERTDSIETS---------------------KMLKK 394

Query: 601 IGVLWKKNQK 610
            GVL KKN K
Sbjct: 601 FGVLLKKNHK 394

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022145717.14.2e-26185.78interactor of constitutive active ROPs 2, chloroplastic-like isoform X2 [Momordi... [more]
XP_022145716.11.7e-25785.48interactor of constitutive active ROPs 2, chloroplastic-like isoform X1 [Momordi... [more]
XP_038905086.12.3e-25480.82interactor of constitutive active ROPs 3-like [Benincasa hispida] >XP_038905087.... [more]
KAG6580589.18.3e-24982.74Interactor of constitutive active ROPs 2, chloroplastic, partial [Cucurbita argy... [more]
XP_022983518.15.4e-24882.58interactor of constitutive active ROPs 2, chloroplastic [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9ZQC53.5e-11748.58Interactor of constitutive active ROPs 2, chloroplastic OS=Arabidopsis thaliana ... [more]
Q9LSS52.9e-10344.63Interactor of constitutive active ROPs 3 OS=Arabidopsis thaliana OX=3702 GN=ICR3... [more]
Q8VYU81.4e-4933.66Interactor of constitutive active ROPs 5 OS=Arabidopsis thaliana OX=3702 GN=ICR5... [more]
F4I8B96.8e-1227.22Putative WEB family protein At1g65010, chloroplastic OS=Arabidopsis thaliana OX=... [more]
F4JJP12.1e-0522.67WEB family protein At4g27595, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Match NameE-valueIdentityDescription
A0A6J1CXH62.1e-26185.78interactor of constitutive active ROPs 2, chloroplastic-like isoform X2 OS=Momor... [more]
A0A6J1CX568.1e-25885.48interactor of constitutive active ROPs 2, chloroplastic-like isoform X1 OS=Momor... [more]
A0A0A0LDX41.3e-25284.15Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G736850 PE=3 SV=1[more]
A0A6J1J7Z22.6e-24882.58interactor of constitutive active ROPs 2, chloroplastic OS=Cucurbita maxima OX=3... [more]
A0A1S3B5P52.6e-24878.28LOW QUALITY PROTEIN: interactor of constitutive active ROPs 3-like OS=Cucumis me... [more]
Match NameE-valueIdentityDescription
AT2G37080.12.5e-11848.58ROP interactive partner 3 [more]
AT5G60210.12.1e-10444.63ROP interactive partner 5 [more]
AT5G60210.22.1e-10444.63ROP interactive partner 5 [more]
AT3G53350.23.1e-5233.77ROP interactive partner 4 [more]
AT3G53350.33.1e-5233.77ROP interactive partner 4 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 273..300
NoneNo IPR availableCOILSCoilCoilcoord: 367..401
NoneNo IPR availableCOILSCoilCoilcoord: 245..265
NoneNo IPR availableCOILSCoilCoilcoord: 425..547
NoneNo IPR availableCOILSCoilCoilcoord: 76..138
NoneNo IPR availableCOILSCoilCoilcoord: 203..230
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..48
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 575..598
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 106..122
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 52..66
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 137..154
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 75..105
NoneNo IPR availablePANTHERPTHR34224:SF4INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTICcoord: 1..609
IPR029688Interactor of constitutive active ROPsPANTHERPTHR34224INTERACTOR OF CONSTITUTIVE ACTIVE ROPS 2, CHLOROPLASTIC-RELATEDcoord: 1..609

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0027086.1Lag0027086.1mRNA