Lag0026589 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0026589
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCytochrome P450, putative
Locationchr10: 39428038 .. 39428343 (-)
RNA-Seq ExpressionLag0026589
SyntenyLag0026589
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GTGATCCTAGTGGGTAGTTTAATATACTACTTGGGTCTTATCACATCGGAGAACATTGGAACCCATCGGATGGAGATGAAGGGACAGAACTTTCGATACATTCCATTTGGGAGTGGGAAGAGAGGCTGCCCTGGTTCTTCTCTTGCCTTGTTGGTTTTTCCATGTGCAATTGCAGCCATGGTGCAGCGGTTTGATTGGAAGATGGACGGCGATGGCGGCGTTGACTTAAAACCTGGGTCGGGATTTGCTGCAGAGATGGCGACACCGCTTGTTTGTTATGCAAACCCTGTTGATGGGACTGATTAA

mRNA sequence

GTGATCCTAGTGGGTAGTTTAATATACTACTTGGGTCTTATCACATCGGAGAACATTGGAACCCATCGGATGGAGATGAAGGGACAGAACTTTCGATACATTCCATTTGGGAGTGGGAAGAGAGGCTGCCCTGGTTCTTCTCTTGCCTTGTTGGTTTTTCCATGTGCAATTGCAGCCATGGTGCAGCGGTTTGATTGGAAGATGGACGGCGATGGCGGCGTTGACTTAAAACCTGGGTCGGGATTTGCTGCAGAGATGGCGACACCGCTTGTTTGTTATGCAAACCCTGTTGATGGGACTGATTAA

Coding sequence (CDS)

GTGATCCTAGTGGGTAGTTTAATATACTACTTGGGTCTTATCACATCGGAGAACATTGGAACCCATCGGATGGAGATGAAGGGACAGAACTTTCGATACATTCCATTTGGGAGTGGGAAGAGAGGCTGCCCTGGTTCTTCTCTTGCCTTGTTGGTTTTTCCATGTGCAATTGCAGCCATGGTGCAGCGGTTTGATTGGAAGATGGACGGCGATGGCGGCGTTGACTTAAAACCTGGGTCGGGATTTGCTGCAGAGATGGCGACACCGCTTGTTTGTTATGCAAACCCTGTTGATGGGACTGATTAA

Protein sequence

VILVGSLIYYLGLITSENIGTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMDGDGGVDLKPGSGFAAEMATPLVCYANPVDGTD
Homology
BLAST of Lag0026589 vs. NCBI nr
Match: XP_038905529.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida])

HSP 1 Score: 162.2 bits (409), Expect = 2.4e-36
Identity = 73/87 (83.91%), Postives = 78/87 (89.66%), Query Frame = 0

Query: 17  ENIGTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKM--DGDGGV 76
           +NIGTHRMEMKGQNFRYIPFGSGKRGCPG+SLALLVFPCAIA MVQRFDWK   DG G +
Sbjct: 456 DNIGTHRMEMKGQNFRYIPFGSGKRGCPGASLALLVFPCAIATMVQRFDWKTSGDGSGNI 515

Query: 77  DLKPGSGFAAEMATPLVCYANPVDGTD 102
           DL PGSGFAAEMATPL CYANP+ G+D
Sbjct: 516 DLTPGSGFAAEMATPLFCYANPIHGSD 542

BLAST of Lag0026589 vs. NCBI nr
Match: XP_004136741.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis sativus] >KAE8651142.1 hypothetical protein Csa_001178 [Cucumis sativus])

HSP 1 Score: 158.3 bits (399), Expect = 3.5e-35
Identity = 72/85 (84.71%), Postives = 78/85 (91.76%), Query Frame = 0

Query: 17  ENIGTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMDGD--GGV 76
           ENIG+H+MEMKGQNFRYIPFGSGKRGCPG+SLALLVFPCAIA MVQRFDWK+DGD  G V
Sbjct: 440 ENIGSHKMEMKGQNFRYIPFGSGKRGCPGASLALLVFPCAIATMVQRFDWKIDGDNEGNV 499

Query: 77  DLKPGSGFAAEMATPLVCYANPVDG 100
           DL  GSGFAAEMATPL+CYANP+ G
Sbjct: 500 DLTVGSGFAAEMATPLICYANPIMG 524

BLAST of Lag0026589 vs. NCBI nr
Match: KAG6580822.1 (3,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 156.8 bits (395), Expect = 1.0e-34
Identity = 72/85 (84.71%), Postives = 78/85 (91.76%), Query Frame = 0

Query: 17  ENIGTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMDGDGG--- 76
           ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFDWK+DGDG    
Sbjct: 431 ENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDWKVDGDGDGNT 490

Query: 77  VDLKPGSGFAAEMATPLVCYANPVD 99
           +DL PG GFAAEMATPLV YANP++
Sbjct: 491 IDLTPGPGFAAEMATPLVRYANPIN 515

BLAST of Lag0026589 vs. NCBI nr
Match: XP_022982904.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima])

HSP 1 Score: 156.8 bits (395), Expect = 1.0e-34
Identity = 72/85 (84.71%), Postives = 78/85 (91.76%), Query Frame = 0

Query: 17  ENIGTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMDGDGG--- 76
           ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFDWK+DGDG    
Sbjct: 442 ENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDWKVDGDGDGDT 501

Query: 77  VDLKPGSGFAAEMATPLVCYANPVD 99
           +DL PG GFAAEMATPLV YANP++
Sbjct: 502 IDLTPGPGFAAEMATPLVRYANPIN 526

BLAST of Lag0026589 vs. NCBI nr
Match: XP_023528514.1 (3,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 156.0 bits (393), Expect = 1.7e-34
Identity = 71/87 (81.61%), Postives = 80/87 (91.95%), Query Frame = 0

Query: 15  TSENIGTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMDGDGG- 74
           ++ENIG+HRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCA+AAMVQRFDWK+DGDG  
Sbjct: 438 SNENIGSHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAMAAMVQRFDWKVDGDGDG 497

Query: 75  --VDLKPGSGFAAEMATPLVCYANPVD 99
             +DL PG GFAAEMATPLV YA+P++
Sbjct: 498 NTIDLTPGPGFAAEMATPLVRYADPIN 524

BLAST of Lag0026589 vs. ExPASy Swiss-Prot
Match: Q42798 (3,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1 SV=1)

HSP 1 Score: 79.7 bits (195), Expect = 2.1e-14
Identity = 33/78 (42.31%), Postives = 53/78 (67.95%), Query Frame = 0

Query: 20  GTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKM-DGDGGVDLKP 79
           G ++++++GQ++ +IPFGSG+R CPG+SLA  V P  +A ++Q F WK+  G+G VD++ 
Sbjct: 424 GQNQLDVRGQHYHFIPFGSGRRTCPGASLAWQVVPVNLAIIIQCFQWKLVGGNGKVDMEE 483

Query: 80  GSGFAAEMATPLVCYANP 97
            SG     A P++C   P
Sbjct: 484 KSGITLPRANPIICVPVP 501

BLAST of Lag0026589 vs. ExPASy Swiss-Prot
Match: Q42799 (Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1)

HSP 1 Score: 78.6 bits (192), Expect = 4.6e-14
Identity = 33/78 (42.31%), Postives = 51/78 (65.38%), Query Frame = 0

Query: 20  GTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMD-GDGGVDLKP 79
           G  +++++GQ++ +IPFGSG+R CPG+SLAL +    +A M+Q F WK D G+  VD++ 
Sbjct: 417 GQSQLDVRGQHYHFIPFGSGRRSCPGTSLALQIVHVNLAIMIQCFQWKFDNGNNKVDMEE 476

Query: 80  GSGFAAEMATPLVCYANP 97
            SG     A P++C   P
Sbjct: 477 KSGITLPRAHPIICVPVP 494

BLAST of Lag0026589 vs. ExPASy Swiss-Prot
Match: O81973 (Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1)

HSP 1 Score: 73.6 bits (179), Expect = 1.5e-12
Identity = 30/74 (40.54%), Postives = 50/74 (67.57%), Query Frame = 0

Query: 20  GTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMDGDGG-VDLKP 79
           G  +++++GQ++  +PFGSG+R CPG+SLAL V    +A ++Q F WK+D D G V+++ 
Sbjct: 425 GKSQLDVRGQHYHLLPFGSGRRACPGTSLALQVVHVNLAVLIQCFQWKVDCDNGKVNMEE 484

Query: 80  GSGFAAEMATPLVC 93
            +G     A P++C
Sbjct: 485 KAGITLPRAHPIIC 498

BLAST of Lag0026589 vs. ExPASy Swiss-Prot
Match: Q0JFI2 (Cytochrome P450 93G1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP93G1 PE=1 SV=1)

HSP 1 Score: 72.4 bits (176), Expect = 3.3e-12
Identity = 30/77 (38.96%), Postives = 48/77 (62.34%), Query Frame = 0

Query: 20  GTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKMDGDGGVDLKPG 79
           G   +E +GQ+F+++PFGSG+RGCPG  LAL   P  +AA++Q FDW+   +  +D++  
Sbjct: 431 GGEGVEPRGQHFQFMPFGSGRRGCPGMGLALQSVPAVVAALLQCFDWQCMDNKLIDMEEA 490

Query: 80  SGFAAEMATPLVCYANP 97
            G        L+ +A+P
Sbjct: 491 DGLVCARKHRLLLHAHP 507

BLAST of Lag0026589 vs. ExPASy Swiss-Prot
Match: Q9XHC6 (Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 1.6e-11
Identity = 33/83 (39.76%), Postives = 53/83 (63.86%), Query Frame = 0

Query: 16  SENIGTHRMEMKGQNFRYIPFGSGKRGCPGSSLALLVFPCAIAAMVQRFDWKM-DG-DGG 75
           S++ G  +++++GQ ++ +PFGSG+R CPG+SLALLV    +A+++Q FDW + DG +  
Sbjct: 424 SDDPGKSKIDVRGQYYQLLPFGSGRRSCPGASLALLVMQATLASLIQCFDWIVNDGKNHH 483

Query: 76  VDLKPGSGFAAEMATPLVCYANP 97
           VD+         +A PL C   P
Sbjct: 484 VDMSEEGRVTVFLAKPLKCKPVP 506

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038905529.12.4e-3683.913,9-dihydroxypterocarpan 6A-monooxygenase [Benincasa hispida][more]
XP_004136741.13.5e-3584.713,9-dihydroxypterocarpan 6A-monooxygenase [Cucumis sativus] >KAE8651142.1 hypoth... [more]
KAG6580822.11.0e-3484.713,9-dihydroxypterocarpan 6A-monooxygenase, partial [Cucurbita argyrosperma subsp... [more]
XP_022982904.11.0e-3484.713,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita maxima][more]
XP_023528514.11.7e-3481.613,9-dihydroxypterocarpan 6A-monooxygenase [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q427982.1e-1442.313,9-dihydroxypterocarpan 6A-monooxygenase OS=Glycine max OX=3847 GN=CYP93A1 PE=1... [more]
Q427994.6e-1442.31Cytochrome P450 93A2 OS=Glycine max OX=3847 GN=CYP93A2 PE=2 SV=1[more]
O819731.5e-1240.54Cytochrome P450 93A3 OS=Glycine max OX=3847 GN=CYP93A3 PE=2 SV=1[more]
Q0JFI23.3e-1238.96Cytochrome P450 93G1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP93G1 PE=1 SV... [more]
Q9XHC61.6e-1139.76Beta-amyrin 24-hydroxylase OS=Glycine max OX=3847 GN=CYP93E1 PE=1 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 33..43
score: 55.79
coord: 43..66
score: 28.13
IPR001128Cytochrome P450PFAMPF00067p450coord: 26..77
e-value: 5.7E-11
score: 41.8
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 16..99
e-value: 1.7E-13
score: 51.9
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 27..94
NoneNo IPR availablePANTHERPTHR24298FLAVONOID 3'-MONOOXYGENASE-RELATEDcoord: 17..94
NoneNo IPR availablePANTHERPTHR24298:SF204CYTOCHROME P450, FAMILY 712, SUBFAMILY A, POLYPEPTIDE 1coord: 17..94
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 36..45

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0026589.1Lag0026589.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen