Lag0026546 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0026546
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pyridoxal 5'-phosphate synthase
Location	chr10: 38869176 .. 38870430 (-)
RNA-Seq Expression	Lag0026546
Synteny	Lag0026546

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGTCAGCAGCAGCGCCATGGAAATCCCTTCTTCTCGGCGCTTTGGAATCTAATGCTCAAATCAAGCATTCCAAATTCTTGCAGCTCGTACAGCTCCCTCACTGATTATTCTCCAAGTTTAATTTCCATTTTTTCCCCTTTTGTAGTCGGTTTATACATGCAATTTGTTTTCAGGCAACGATCGGAACAAATGGAAGGCCCTCAAATCGCACAGTTGTTTTCAGAGGATTTGAAGACGACACTGATAAGATCCAAATTCACACGAATTCTGGAAGTCGCAAGGTTCTTTTAAGAGGAAGCTATATATTTCGTTTTAAGTCGCTCAATGCATATAGCCATTTGTTGTTGTTCTATTGTTTTTGAAGCTATGATTGTCTGGTTTTCTGGAATTTGAGTGCAATCTGTTATTGGCGTTGGTGTTTTTGATTGTTTGTGATGGATTAGGTGTGTGATAATTAAGCGTATGAGTTTTGTGCTGAAAATGGACTTCTCTTTTGGTTGATTCTACAGATTGAAGAGCTTAAGAATAGTCCATTTGATGAGGTAAAGTTAAAGTTACTGTATTTGGTTGTTAGGACAGATGATATTGACCTGGGTATCAATTTCTTGTGTTAGTTGAAATATTTCATTTATCATTCTCAAGCTAGGAGGTTTTGTAGCTTTATAATCTTAGGGAAATTTGGCGATATTTTCAAATTTGTCAAATTATAAGTTTAGTCCTTGAACTTTTAGAGATATGCCTATTTGGTTCTTGAACTTTAAAAAGTGTCTAATATGTATTTGTGTTCAATAGGTCCCTGAACTTTAAAAAATATCTAATAGATCCTTAAAGTTTCAATATATCAATTCTTAACTTTGAAAGTTGTAAACAATTTAGTCTCGGAACATTAGTGTGTAACAATTTAGTCTTCATCTATGAAGCACGAACACGTGAAAATAAGAGAAGAATTTGTGTCGGACACGTGTCGGATACGCAAATTTGCGTGTCTTTTTTTTCTTTTTCTTTTTTTAAAATTTTATTATTATTATTATTATTTTTTTAAATTATGGACATGTTGGGACATGCCAATGAACATGCACGCCTAGCCCAGGTAAAGCCCAAAATTTTGGGCTTCTCTTTCGAAAACAACCGTCAGCAAAGCTTCTTCATGATTTCATTTGGGCATTTTCCTTCCCTCCATCTTTGGCACCGTCGGCGTTGTCAGCCATTTTCGCCACCGCCATCACCGGTCGTCTTCGCTGCTGTCGCTAG

mRNA sequence

ATGGGGTCAGCAGCAGCGCCATGGAAATCCCTTCTTCTCGGCGCTTTGGAATCTAATGCTCAAATCAAGCATTCCAAATTCTTGCAGCTCGCAACGATCGGAACAAATGGAAGGCCCTCAAATCGCACAGTTGTTTTCAGAGGATTTGAAGACGACACTGATAAGATCCAAATTCACACGAATTCTGGAAGTCGCAAGATTGAAGAGCTTAAGAATAGTCCATTTGATGAGGTAAAGTTAAAGTTACTGTATTTGGTTGTTAGGACAGATGATATTGACCTGGGTAAAGCCCAAAATTTTGGGCTTCTCTTTCGAAAACAACCGTCAGCAAAGCTTCTTCATGATTTCATTTGGGCATTTTCCTTCCCTCCATCTTTGGCACCGTCGGCGTTGTCAGCCATTTTCGCCACCGCCATCACCGGTCGTCTTCGCTGCTGTCGCTAG

Coding sequence (CDS)

ATGGGGTCAGCAGCAGCGCCATGGAAATCCCTTCTTCTCGGCGCTTTGGAATCTAATGCTCAAATCAAGCATTCCAAATTCTTGCAGCTCGCAACGATCGGAACAAATGGAAGGCCCTCAAATCGCACAGTTGTTTTCAGAGGATTTGAAGACGACACTGATAAGATCCAAATTCACACGAATTCTGGAAGTCGCAAGATTGAAGAGCTTAAGAATAGTCCATTTGATGAGGTAAAGTTAAAGTTACTGTATTTGGTTGTTAGGACAGATGATATTGACCTGGGTAAAGCCCAAAATTTTGGGCTTCTCTTTCGAAAACAACCGTCAGCAAAGCTTCTTCATGATTTCATTTGGGCATTTTCCTTCCCTCCATCTTTGGCACCGTCGGCGTTGTCAGCCATTTTCGCCACCGCCATCACCGGTCGTCTTCGCTGCTGTCGCTAG

Protein sequence

MGSAAAPWKSLLLGALESNAQIKHSKFLQLATIGTNGRPSNRTVVFRGFEDDTDKIQIHTNSGSRKIEELKNSPFDEVKLKLLYLVVRTDDIDLGKAQNFGLLFRKQPSAKLLHDFIWAFSFPPSLAPSALSAIFATAITGRLRCCR

Homology

[Close]

BLAST of Lag0026546 vs. NCBI nr
Match: XP_038899249.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 132.1 bits (331), Expect = 3.9e-27
Identity = 65/78 (83.33%), Postives = 71/78 (91.03%), Query Frame = 0


Query: 1  MGSAAAPWKSLLLGALESNAQIKHSKFLQLATIGTNGRPSNRTVVFRGFEDDTDKIQIHT 60
          MGSAAAPWKSLLLGALESN +IKHSKFLQLATIG NGRPSNRTVVFRGFE+ TDKI I+T
Sbjct: 1  MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDKIHIYT 60

Query: 61 NSGSRKIEELKNSPFDEV 79
          +S +RKIEELKN PF E+
Sbjct: 61 DSRTRKIEELKNCPFAEI 78

[Close]

BLAST of Lag0026546 vs. NCBI nr
Match: XP_038874699.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X2 [Benincasa hispida])

HSP 1 Score: 131.0 bits (328), Expect = 8.7e-27
Identity = 64/78 (82.05%), Postives = 71/78 (91.03%), Query Frame = 0


Query: 1  MGSAAAPWKSLLLGALESNAQIKHSKFLQLATIGTNGRPSNRTVVFRGFEDDTDKIQIHT 60
          MGSAAAPWKSLLLGALESN +IKHSKFLQLATIG NGRPSNRTVVFRGFE+ TD+I I+T
Sbjct: 1  MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDRIHIYT 60

Query: 61 NSGSRKIEELKNSPFDEV 79
          +S +RKIEELKN PF E+
Sbjct: 61 DSRTRKIEELKNCPFAEI 78

[Close]

BLAST of Lag0026546 vs. NCBI nr
Match: XP_038899256.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 131.0 bits (328), Expect = 8.7e-27
Identity = 65/77 (84.42%), Postives = 70/77 (90.91%), Query Frame = 0


Query: 1  MGSAAAPWKSLLLGALESNAQIKHSKFLQLATIGTNGRPSNRTVVFRGFEDDTDKIQIHT 60
          MGSAAAPWKSLLLGALESN +IKHSKFLQLATIG NGRPSNRTVVFRGFE+ TDKI I+T
Sbjct: 1  MGSAAAPWKSLLLGALESNGRIKHSKFLQLATIGINGRPSNRTVVFRGFEEGTDKIHIYT 60

Query: 61 NSGSRKIEELKNSPFDE 78
          +S +RKIEELKN PF E
Sbjct: 61 DSRTRKIEELKNCPFAE 77

[Close]

BLAST of Lag0026546 vs. NCBI nr
Match: XP_008437871.1 (PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >XP_016898998.1 PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >TYK20828.1 pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 128.6 bits (322), Expect = 4.3e-26
Identity = 65/78 (83.33%), Postives = 69/78 (88.46%), Query Frame = 0


Query: 1  MGSAAAPWKSLLLGALESNAQIKHSKFLQLATIGTNGRPSNRTVVFRGFEDDTDKIQIHT 60
          MGSA APWKSLLL ALESN QIKHSKFLQLATIGTNGRPSNRTVVFR FE+ TD+I I+T
Sbjct: 1  MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61 NSGSRKIEELKNSPFDEV 79
          +S SRKIEELKN PF EV
Sbjct: 61 DSRSRKIEELKNCPFAEV 78

[Close]

BLAST of Lag0026546 vs. NCBI nr
Match: KAA0048877.1 (pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa])

HSP 1 Score: 128.3 bits (321), Expect = 5.6e-26
Identity = 64/78 (82.05%), Postives = 69/78 (88.46%), Query Frame = 0


Query: 1  MGSAAAPWKSLLLGALESNAQIKHSKFLQLATIGTNGRPSNRTVVFRGFEDDTDKIQIHT 60
          MGSA APWKSLLL ALESN QIKHSKFLQLATIGTNGRPSNRTVVFR FE+ TD+I I+T
Sbjct: 1  MGSAVAPWKSLLLRALESNGQIKHSKFLQLATIGTNGRPSNRTVVFREFEEGTDRIHIYT 60

Query: 61 NSGSRKIEELKNSPFDEV 79
          +S SRKIEELKN PF E+
Sbjct: 61 DSRSRKIEELKNCPFAEI 78

[Close]

BLAST of Lag0026546 vs. ExPASy Swiss-Prot
Match: Q9ZPY1 (Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=PPOX2 PE=1 SV=2)

HSP 1 Score: 111.7 bits (278), Expect = 7.1e-24
Identity = 51/78 (65.38%), Postives = 66/78 (84.62%), Query Frame = 0


Query: 1  MGSAAAPWKSLLLGALESNAQIKHSKFLQLATIGTNGRPSNRTVVFRGFEDDTDKIQIHT 60
          MG+  APWK LL GA+E+N+ + HS ++QLATIG NGRPSNRTVVFRGFE+++D+IQI+T
Sbjct: 1  MGTHVAPWKQLLFGAIEANSHLSHSSYVQLATIGLNGRPSNRTVVFRGFEENSDRIQINT 60

Query: 61 NSGSRKIEELKNSPFDEV 79
          +  SRKIEELK+ PF E+
Sbjct: 61 DLRSRKIEELKHCPFSEM 78

The following BLAST results are available for this feature:

BLAST of Lag0026546 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038899249.1	3.9e-27	83.33	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X1 [Benincasa hispida]	[more]
XP_038874699.1	8.7e-27	82.05	pyridoxine/pyridoxamine 5'-phosphate oxidase 2-like isoform X2 [Benincasa hispid...	[more]
XP_038899256.1	8.7e-27	84.42	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 isoform X2 [Benincasa hispida]	[more]
XP_008437871.1	4.3e-26	83.33	PREDICTED: pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo] >XP_016...	[more]
KAA0048877.1	5.6e-26	82.05	pyridoxine/pyridoxamine 5'-phosphate oxidase 2 [Cucumis melo var. makuwa]	[more]

BLAST of Lag0026546 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZPY1	7.1e-24	65.38	Pyridoxine/pyridoxamine 5'-phosphate oxidase 2 OS=Arabidopsis thaliana OX=3702 G...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR024624	Pyridoxamine 5'-phosphate oxidase, Alr4036 family, FMN-binding domain	PFAM	PF12766	Pyridox_oxase_2	coord: 6..74 e-value: 4.2E-14 score: 53.0
IPR012349	FMN-binding split barrel	GENE3D	2.30.110.10		coord: 3..83 e-value: 4.5E-21 score: 77.6
IPR000659	Pyridoxamine 5'-phosphate oxidase	PANTHER	PTHR10851	PYRIDOXINE-5-PHOSPHATE OXIDASE	coord: 2..78
None	No IPR available	PANTHER	PTHR10851:SF3	PYRIDOXINE/PYRIDOXAMINE 5'-PHOSPHATE OXIDASE 2	coord: 2..78
None	No IPR available	SUPERFAMILY	50475	FMN-binding split barrel	coord: 3..75

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0026546.1	Lag0026546.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042823	pyridoxal phosphate biosynthetic process
biological_process	GO:0008615	pyridoxine biosynthetic process
molecular_function	GO:0010181	FMN binding
molecular_function	GO:0004733	pyridoxamine-phosphate oxidase activity