Lag0025812 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0025812
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Germin-like protein
Location	chr10: 21419074 .. 21419697 (+)
RNA-Seq Expression	Lag0025812
Synteny	Lag0025812

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTTCCTTTCTACAAAATCAGCTACATTATTCTTCTTCTTCATTTTAGCCATATTTTCAATGGCCGAAGCCGGCGACCCAGATCTGATCGTCGATTTCCTCAACCCACCAGACGCCACCCTCAACAGCACTTTCTTCACCTTCACAGGCCTTCGCTCAGCCTTCGCCGCCTTCCCTCCAAATTTCAAGCCCACAAAAGCCACCTTCACCGAGTTCCCCGCCCTTCTCGGCCAGAGCGTCGCCTTGGCCGTCCTCCAGTACCCTGCCGGCGCCCTCAACCCGCCGCACACCCACCCCCGCTCCGCCGAACTCCTCCTCGTCATCGGCGGCACCCTGCAGGTGGGCTTCGTCGACACTACCAACAAGCTCTTCAATCAGACCCTTCAGCTTGGGGACGTGTTTCTGTTCCCCAAAGGGTTGGTCCATTTCCAGTTCAATGCTGACCCTCGAAATTCTGCCACTGCCATCGCCGCCTTCGGCAGCGCCAACCCCGGCGCCGTTTCGCTGCCACTCTCGGTGTTTACCAGTGGGATCAGTGATAAGGTTCTTGCTAAGGCCTTTAAGACTGATGTGACTGTTATTGAGAGTATTAAGGCCGGCCTTGCACCACCACCAAGTTAA

mRNA sequence

ATGGCTTTCCTTTCTACAAAATCAGCTACATTATTCTTCTTCTTCATTTTAGCCATATTTTCAATGGCCGAAGCCGGCGACCCAGATCTGATCGTCGATTTCCTCAACCCACCAGACGCCACCCTCAACAGCACTTTCTTCACCTTCACAGGCCTTCGCTCAGCCTTCGCCGCCTTCCCTCCAAATTTCAAGCCCACAAAAGCCACCTTCACCGAGTTCCCCGCCCTTCTCGGCCAGAGCGTCGCCTTGGCCGTCCTCCAGTACCCTGCCGGCGCCCTCAACCCGCCGCACACCCACCCCCGCTCCGCCGAACTCCTCCTCGTCATCGGCGGCACCCTGCAGGTGGGCTTCGTCGACACTACCAACAAGCTCTTCAATCAGACCCTTCAGCTTGGGGACGTGTTTCTGTTCCCCAAAGGGTTGGTCCATTTCCAGTTCAATGCTGACCCTCGAAATTCTGCCACTGCCATCGCCGCCTTCGGCAGCGCCAACCCCGGCGCCGTTTCGCTGCCACTCTCGGTGTTTACCAGTGGGATCAGTGATAAGGTTCTTGCTAAGGCCTTTAAGACTGATGTGACTGTTATTGAGAGTATTAAGGCCGGCCTTGCACCACCACCAAGTTAA

Coding sequence (CDS)

ATGGCTTTCCTTTCTACAAAATCAGCTACATTATTCTTCTTCTTCATTTTAGCCATATTTTCAATGGCCGAAGCCGGCGACCCAGATCTGATCGTCGATTTCCTCAACCCACCAGACGCCACCCTCAACAGCACTTTCTTCACCTTCACAGGCCTTCGCTCAGCCTTCGCCGCCTTCCCTCCAAATTTCAAGCCCACAAAAGCCACCTTCACCGAGTTCCCCGCCCTTCTCGGCCAGAGCGTCGCCTTGGCCGTCCTCCAGTACCCTGCCGGCGCCCTCAACCCGCCGCACACCCACCCCCGCTCCGCCGAACTCCTCCTCGTCATCGGCGGCACCCTGCAGGTGGGCTTCGTCGACACTACCAACAAGCTCTTCAATCAGACCCTTCAGCTTGGGGACGTGTTTCTGTTCCCCAAAGGGTTGGTCCATTTCCAGTTCAATGCTGACCCTCGAAATTCTGCCACTGCCATCGCCGCCTTCGGCAGCGCCAACCCCGGCGCCGTTTCGCTGCCACTCTCGGTGTTTACCAGTGGGATCAGTGATAAGGTTCTTGCTAAGGCCTTTAAGACTGATGTGACTGTTATTGAGAGTATTAAGGCCGGCCTTGCACCACCACCAAGTTAA

Protein sequence

MAFLSTKSATLFFFFILAIFSMAEAGDPDLIVDFLNPPDATLNSTFFTFTGLRSAFAAFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTSGISDKVLAKAFKTDVTVIESIKAGLAPPPS

Homology

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BLAST of Lag0025812 vs. NCBI nr
Match: XP_038893494.1 (putative germin-like protein 9-2 [Benincasa hispida])

HSP 1 Score: 319.7 bits (818), Expect = 1.9e-83
Identity = 161/208 (77.40%), Postives = 182/208 (87.50%), Query Frame = 0


Query: 1   MAFLSTKSATLFFFFILAIFSMAEAGDPDLIVDFLNPP-DATLNSTFFTFTGLRSAFAAF 60
           MAF STKS TLFFFFIL IFS+A++ DPD IVDF + P  A +N + FTFTGLRSAF  F
Sbjct: 1   MAFPSTKSTTLFFFFILTIFSIAKSSDPDPIVDFFSSPLGAIINGSSFTFTGLRSAFTDF 60

Query: 61  PPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVD 120
           PPNFKPTKATF+EFPALLGQSV++A+LQYPAG +NPPHTHPRSAELLLV+ G+LQVGF+D
Sbjct: 61  PPNFKPTKATFSEFPALLGQSVSMAILQYPAGTINPPHTHPRSAELLLVVSGSLQVGFID 120

Query: 121 TTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTSGI 180
           TTN+ FNQTLQ+GD+F+FPKGLVHFQFNADP NSATAIAAF SANPG VSLP +VFTSGI
Sbjct: 121 TTNQFFNQTLQVGDLFVFPKGLVHFQFNADPGNSATAIAAFASANPGTVSLPPTVFTSGI 180

Query: 181 SDKVLAKAFKTDVTVIESIKAGLAPPPS 208
           SD+VLA+AFKTDV VI SIK GL PP S
Sbjct: 181 SDEVLAEAFKTDVAVIRSIKVGLTPPKS 208

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BLAST of Lag0025812 vs. NCBI nr
Match: XP_008463986.1 (PREDICTED: germin-like protein 9-3 [Cucumis melo] >KAA0035508.1 germin-like protein 9-3 [Cucumis melo var. makuwa] >TYK31021.1 germin-like protein 9-3 [Cucumis melo var. makuwa])

HSP 1 Score: 318.5 bits (815), Expect = 4.1e-83
Identity = 161/210 (76.67%), Postives = 184/210 (87.62%), Query Frame = 0


Query: 1   MAFLSTKSAT--LFFFFILAIFSMAEAGDPDLIVDFLNPP-DATLNSTFFTFTGLRSAFA 60
           MAFLSTK AT   FFFFIL+IFS A++GDPD +VD+L  P  A +NSTFFTFTGLRSAF 
Sbjct: 1   MAFLSTKPATFFFFFFFILSIFSFAKSGDPDPVVDYLTSPLGAIINSTFFTFTGLRSAFT 60

Query: 61  AFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGF 120
            FPPNFKPTKATF EFPALL QSV++A+LQYPAG++NPPHTHPRSAELLLV+ G+LQVGF
Sbjct: 61  DFPPNFKPTKATFNEFPALLSQSVSMAILQYPAGSINPPHTHPRSAELLLVVSGSLQVGF 120

Query: 121 VDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTS 180
           VDT+N+ FNQTLQ+GD+FLFPKGLVHFQFNADP+NSATAIA F SAN G VSLP +VFTS
Sbjct: 121 VDTSNQFFNQTLQVGDLFLFPKGLVHFQFNADPQNSATAIATFASANAGTVSLPSTVFTS 180

Query: 181 GISDKVLAKAFKTDVTVIESIKAGLAPPPS 208
           GISD+VLA+AFKTDV +I+SIK GL PP S
Sbjct: 181 GISDEVLAEAFKTDVAIIQSIKTGLTPPKS 210

[Close]

BLAST of Lag0025812 vs. NCBI nr
Match: XP_031736138.1 (putative germin-like protein 9-2 [Cucumis sativus])

HSP 1 Score: 311.6 bits (797), Expect = 5.0e-81
Identity = 160/212 (75.47%), Postives = 183/212 (86.32%), Query Frame = 0


Query: 1   MAFLSTKSAT---LFFFFILAIFSMAEAGDPDLIVDFLNPP-DATLNSTFFTFTGLRSAF 60
           MAFLSTKS T    FFFFIL+IFSMA++ DPD +VD+L  P DA +N TFFTFTGLRSAF
Sbjct: 1   MAFLSTKSTTFFFFFFFFILSIFSMAKSSDPDPVVDYLTSPLDAIINGTFFTFTGLRSAF 60

Query: 61  AAFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVG 120
             FP NFKPTKATF+EFPALL QSV++A+LQYPAG++NPPH+HPRSAELLLV+ G+LQVG
Sbjct: 61  TDFPSNFKPTKATFSEFPALLSQSVSMAILQYPAGSINPPHSHPRSAELLLVVSGSLQVG 120

Query: 121 FVDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLS-VF 180
           FVDT N+ FNQTLQ+GD+FLFPKGLVHFQ N DPRNSATAIA F SANPG V+LP S VF
Sbjct: 121 FVDTANQFFNQTLQVGDLFLFPKGLVHFQLNVDPRNSATAIATFASANPGTVALPSSTVF 180

Query: 181 TSGISDKVLAKAFKTDVTVIESIKAGLAPPPS 208
           TSGISD+VLA+AFKTDV VI+SI+AGL PP S
Sbjct: 181 TSGISDEVLAEAFKTDVAVIQSIRAGLTPPKS 212

[Close]

BLAST of Lag0025812 vs. NCBI nr
Match: XP_022142670.1 (putative germin-like protein 9-2 [Momordica charantia])

HSP 1 Score: 287.3 bits (734), Expect = 1.0e-73
Identity = 148/208 (71.15%), Postives = 170/208 (81.73%), Query Frame = 0


Query: 4   LSTKSATLFFFFILAI-----FSMAEAGDPDLIVDFLNPPDATLNSTFFTFTGLRSAFAA 63
           ++  S+   FF ++A+     ++MA+AGD DLIVDF+ P D   N +FFTFTGLR A A 
Sbjct: 1   MAMASSYFLFFVVVAMILSSCWNMADAGDADLIVDFIGPLDGGANGSFFTFTGLRFAVAG 60

Query: 64  -FPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGF 123
            FPPNFKPTKATF EFPALLGQSV+L VLQ+PAGA+NPPHTHPRSAELL ++ GTLQVGF
Sbjct: 61  EFPPNFKPTKATFLEFPALLGQSVSLTVLQFPAGAVNPPHTHPRSAELLFLLSGTLQVGF 120

Query: 124 VDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTS 183
           VDTTNKLFNQTLQ+GD FLFPKGL+HFQFN+DP NSATAIAAFGSAN G VS+PLSVF+S
Sbjct: 121 VDTTNKLFNQTLQVGDXFLFPKGLLHFQFNSDPLNSATAIAAFGSANAGTVSVPLSVFSS 180

Query: 184 GISDKVLAKAFKTDVTVIESIKAGLAPP 206
           GISD VLAKAF T V V+  IK GL PP
Sbjct: 181 GISDDVLAKAFNTHVAVVRRIKHGLTPP 208

[Close]

BLAST of Lag0025812 vs. NCBI nr
Match: XP_004139583.2 (putative germin-like protein 9-2 [Cucumis sativus])

HSP 1 Score: 283.5 bits (724), Expect = 1.5e-72
Identity = 140/186 (75.27%), Postives = 162/186 (87.10%), Query Frame = 0


Query: 24  EAGDPDLIVDFLNPP-DATLNSTFFTFTGLRSAFAAFPPNFKPTKATFTEFPALLGQSVA 83
           ++ DPD +VD+L  P DA +N TFFTFTGLRSAF  FP NFKPTKATF+EFPALL QSV+
Sbjct: 29  KSSDPDPVVDYLTSPLDAIINGTFFTFTGLRSAFTDFPSNFKPTKATFSEFPALLSQSVS 88

Query: 84  LAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDTTNKLFNQTLQLGDVFLFPKGLV 143
           +A+LQYPAG++NPPH+HPRSAELLLV+ G+LQVGFVDT N+ FNQTLQ+GD+FLFPKGLV
Sbjct: 89  MAILQYPAGSINPPHSHPRSAELLLVVSGSLQVGFVDTANQFFNQTLQVGDLFLFPKGLV 148

Query: 144 HFQFNADPRNSATAIAAFGSANPGAVSLPLS-VFTSGISDKVLAKAFKTDVTVIESIKAG 203
           HFQ N DPRNSATAIA F SANPG V+LP S VFTSGISD+VLA+AFKTDV VI+SI+AG
Sbjct: 149 HFQLNVDPRNSATAIATFASANPGTVALPSSTVFTSGISDEVLAEAFKTDVAVIQSIRAG 208

Query: 204 LAPPPS 208
           L PP S
Sbjct: 209 LTPPKS 214

[Close]

BLAST of Lag0025812 vs. ExPASy Swiss-Prot
Match: Q652Q0 (Putative germin-like protein 9-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568600 PE=3 SV=1)

HSP 1 Score: 206.1 bits (523), Expect = 3.9e-52
Identity = 106/192 (55.21%), Postives = 133/192 (69.27%), Query Frame = 0


Query: 21  SMAEAGDPDLIVDFLNPPDA---TLNSTFFTFTGLRSAF----AAFPPNFKPTKATFTEF 80
           ++  AGDPD++ D++ P +     +   FFTFTG R  F    A  P +F  TKAT  EF
Sbjct: 21  TLVMAGDPDILTDYVIPANGNPMNITGDFFTFTGFRKVFNTSSAPEPNSFTVTKATMAEF 80

Query: 81  PALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDTTNKLFNQTLQLGD 140
           PAL GQSV+ A L +P   +NPPHTHPRSAELLLV+ G L VGF+DTTNKL+ Q L  GD
Sbjct: 81  PALNGQSVSYATLVFPPSTVNPPHTHPRSAELLLVVDGALSVGFIDTTNKLYTQDLAAGD 140

Query: 141 VFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTSGISDKVLAKAFKTDVT 200
           +F+FPKG+VHFQFN+     A A++AFGSA PG V +P++VF +GI D VLAK+FKTDV 
Sbjct: 141 MFVFPKGMVHFQFNSG-NQPAMALSAFGSAAPGVVPVPVTVFGTGIDDAVLAKSFKTDVP 200

Query: 201 VIESIKAGLAPP 206
            I  +KA L PP
Sbjct: 201 TILKLKANLTPP 211

[Close]

BLAST of Lag0025812 vs. ExPASy Swiss-Prot
Match: Q652P9 (Germin-like protein 9-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568700 PE=2 SV=1)

HSP 1 Score: 204.5 bits (519), Expect = 1.1e-51
Identity = 111/211 (52.61%), Postives = 144/211 (68.25%), Query Frame = 0


Query: 8   SATLFFFFILAIFS----MAEAGDPDLIVDFLNPPDA---TLNSTFFTFTGLRSAFA--- 67
           ++++    +LA+ S    +  AGDPD++ D++ P  +    +   FFTFTG R+  +   
Sbjct: 2   ASSILLLVVLAVVSAPVALVMAGDPDILTDYVIPAGSNAENITGDFFTFTGFRNPLSMNM 61

Query: 68  AFP---PNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQ 127
           + P    NF  TKAT  EFPAL GQSV+ AVL YP   LNPPHTHPRSAELLL++ G L 
Sbjct: 62  SMPMPNANFTVTKATMAEFPALNGQSVSYAVLMYPPATLNPPHTHPRSAELLLLVDGALS 121

Query: 128 VGFVDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSV 187
           VGFVDTTNKL+ Q L  GD+F+FPKG+VHFQFN+     A A++AFGSA  G VS+P++V
Sbjct: 122 VGFVDTTNKLYTQDLAAGDMFVFPKGMVHFQFNSG-NQPAMALSAFGSAAAGLVSVPVTV 181

Query: 188 FTSGISDKVLAKAFKTDVTVIESIKAGLAPP 206
           F + I D VLAK+FKTDV  I+ +KAGL PP
Sbjct: 182 FGTNIDDAVLAKSFKTDVPTIQKLKAGLTPP 211

[Close]

BLAST of Lag0025812 vs. ExPASy Swiss-Prot
Match: Q652Q1 (Germin-like protein 9-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568500 PE=2 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 3.9e-44
Identity = 97/210 (46.19%), Postives = 130/210 (61.90%), Query Frame = 0


Query: 1   MAFLSTKSATLFFFFILAIFSMAEAGDPDLIVDFLNPPD---ATLNSTFFTFTGLRSAFA 60
           M   S  S +L    +LA+   A A DPD++ DF+ P D   + ++  FFT+  L +  +
Sbjct: 1   MMMSSRSSVSLGVLLLLAVILSAGAADPDILTDFVVPSDTDPSGIDGAFFTYKNLVTGNS 60

Query: 61  AFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGF 120
             P     TKAT  EFPALLGQSV+ A L + AG +NPPH HPR++ELL+V+ G L VG 
Sbjct: 61  GDPAKLTVTKATHAEFPALLGQSVSYAALVFGAGTVNPPHIHPRASELLVVVQGPLLVGL 120

Query: 121 VDTTNK--LFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVF 180
           VD      ++ QTLQ GD+F+FPKG+VHFQFN      A A +AFGSA PG +SLP ++F
Sbjct: 121 VDAARNGTVYTQTLQTGDMFVFPKGMVHFQFNNGTDVVARAFSAFGSATPGTISLPAALF 180

Query: 181 TSGISDKVLAKAFKTDVTVIESIKAGLAPP 206
            SGI D +L K+  TD   ++ +K   APP
Sbjct: 181 GSGIDDTILDKSMHTDQATVDQLKQDQAPP 210

[Close]

BLAST of Lag0025812 vs. ExPASy Swiss-Prot
Match: Q7F731 (Germin-like protein 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=GER4 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.0e-28
Identity = 77/200 (38.50%), Postives = 112/200 (56.00%), Query Frame = 0


Query: 16  ILAIFSMAEAGDPDLIVDF----------LN--PPDATLNSTFFTFTGLRS-AFAAFPPN 75
           +LA+ + + AGDPD++ D           LN  P  A + +  F F GL++      P  
Sbjct: 15  VLALAAPSLAGDPDMLQDVCVADLASPVKLNGFPCKANVTADDFFFAGLKNPGNTNNPAG 74

Query: 76  FKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDTTN 135
              T A    FP +    V++A + Y  G  NPPHTHPR+ E++ V+ G L+VGF+ T N
Sbjct: 75  SNVTAANVQSFPGVNTLGVSMARIDYAPGGQNPPHTHPRATEIIFVLEGVLEVGFITTAN 134

Query: 136 KLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTSG--IS 195
           KLF +T+  G+VF+FP+GLVHFQ N      A  IAAF S   G  ++  ++F +   + 
Sbjct: 135 KLFTKTVTAGEVFVFPRGLVHFQQNRG-HGPAAVIAAFNSQLQGTQAIAATLFAAAPPVP 194

Query: 196 DKVLAKAFKTDVTVIESIKA 201
             VLAKAF+ DV  +++IKA
Sbjct: 195 SDVLAKAFRVDVPQVDAIKA 213

[Close]

BLAST of Lag0025812 vs. ExPASy Swiss-Prot
Match: Q9M263 (Germin-like protein subfamily 2 member 4 OS=Arabidopsis thaliana OX=3702 GN=GLP10 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.9e-28
Identity = 77/210 (36.67%), Postives = 113/210 (53.81%), Query Frame = 0


Query: 12  FFFFILAIFSMAEAGDPDLIVD--------------FLNPPDATLNSTFFTFTGL-RSAF 71
           FFF +L++  +  A DPD + D              F   P++ + ++ F F G+ + A 
Sbjct: 8   FFFTLLSLNVIVLAYDPDTLQDLCVADRTSGIKVNGFTCKPESNITASDFFFAGIGKPAV 67

Query: 72  AAFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVG 131
                    T A   +   L    V+LA + Y  G LNPPHTHPR+ E++ V+ G L VG
Sbjct: 68  VNNTVGSAVTGANVEKIAGLNTLGVSLARIDYAPGGLNPPHTHPRATEVIFVLEGELDVG 127

Query: 132 FVDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFT 191
           F+ T NKLF +T++ G+VF+FP+GL+H+Q N D    A+ I+AF S  PG  S+  ++FT
Sbjct: 128 FITTANKLFAKTVKKGEVFVFPRGLIHYQKNNDKAKPASVISAFNSQLPGTQSIAATLFT 187

Query: 192 S--GISDKVLAKAFKTDVTVIESIKAGLAP 205
           +   I D VL   F+     IE IK+  AP
Sbjct: 188 ATPAIPDHVLTTTFQIGTKEIEKIKSKFAP 217

[Close]

BLAST of Lag0025812 vs. ExPASy TrEMBL
Match: A0A5A7SY36 (Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G002710 PE=3 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 2.0e-83
Identity = 161/210 (76.67%), Postives = 184/210 (87.62%), Query Frame = 0


Query: 1   MAFLSTKSAT--LFFFFILAIFSMAEAGDPDLIVDFLNPP-DATLNSTFFTFTGLRSAFA 60
           MAFLSTK AT   FFFFIL+IFS A++GDPD +VD+L  P  A +NSTFFTFTGLRSAF 
Sbjct: 1   MAFLSTKPATFFFFFFFILSIFSFAKSGDPDPVVDYLTSPLGAIINSTFFTFTGLRSAFT 60

Query: 61  AFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGF 120
            FPPNFKPTKATF EFPALL QSV++A+LQYPAG++NPPHTHPRSAELLLV+ G+LQVGF
Sbjct: 61  DFPPNFKPTKATFNEFPALLSQSVSMAILQYPAGSINPPHTHPRSAELLLVVSGSLQVGF 120

Query: 121 VDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTS 180
           VDT+N+ FNQTLQ+GD+FLFPKGLVHFQFNADP+NSATAIA F SAN G VSLP +VFTS
Sbjct: 121 VDTSNQFFNQTLQVGDLFLFPKGLVHFQFNADPQNSATAIATFASANAGTVSLPSTVFTS 180

Query: 181 GISDKVLAKAFKTDVTVIESIKAGLAPPPS 208
           GISD+VLA+AFKTDV +I+SIK GL PP S
Sbjct: 181 GISDEVLAEAFKTDVAIIQSIKTGLTPPKS 210

[Close]

BLAST of Lag0025812 vs. ExPASy TrEMBL
Match: A0A1S3CKF8 (Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103501975 PE=3 SV=1)

HSP 1 Score: 318.5 bits (815), Expect = 2.0e-83
Identity = 161/210 (76.67%), Postives = 184/210 (87.62%), Query Frame = 0


Query: 1   MAFLSTKSAT--LFFFFILAIFSMAEAGDPDLIVDFLNPP-DATLNSTFFTFTGLRSAFA 60
           MAFLSTK AT   FFFFIL+IFS A++GDPD +VD+L  P  A +NSTFFTFTGLRSAF 
Sbjct: 1   MAFLSTKPATFFFFFFFILSIFSFAKSGDPDPVVDYLTSPLGAIINSTFFTFTGLRSAFT 60

Query: 61  AFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGF 120
            FPPNFKPTKATF EFPALL QSV++A+LQYPAG++NPPHTHPRSAELLLV+ G+LQVGF
Sbjct: 61  DFPPNFKPTKATFNEFPALLSQSVSMAILQYPAGSINPPHTHPRSAELLLVVSGSLQVGF 120

Query: 121 VDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTS 180
           VDT+N+ FNQTLQ+GD+FLFPKGLVHFQFNADP+NSATAIA F SAN G VSLP +VFTS
Sbjct: 121 VDTSNQFFNQTLQVGDLFLFPKGLVHFQFNADPQNSATAIATFASANAGTVSLPSTVFTS 180

Query: 181 GISDKVLAKAFKTDVTVIESIKAGLAPPPS 208
           GISD+VLA+AFKTDV +I+SIK GL PP S
Sbjct: 181 GISDEVLAEAFKTDVAIIQSIKTGLTPPKS 210

[Close]

BLAST of Lag0025812 vs. ExPASy TrEMBL
Match: A0A0A0LSQ3 (Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G166250 PE=3 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 5.8e-75
Identity = 141/187 (75.40%), Postives = 164/187 (87.70%), Query Frame = 0


Query: 22  MAEAGDPDLIVDFLNPP-DATLNSTFFTFTGLRSAFAAFPPNFKPTKATFTEFPALLGQS 81
           MA++ DPD +VD+L  P DA +N TFFTFTGLRSAF  FP NFKPTKATF+EFPALL QS
Sbjct: 1   MAKSSDPDPVVDYLTSPLDAIINGTFFTFTGLRSAFTDFPSNFKPTKATFSEFPALLSQS 60

Query: 82  VALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDTTNKLFNQTLQLGDVFLFPKG 141
           V++A+LQYPAG++NPPH+HPRSAELLLV+ G+LQVGFVDT N+ FNQTLQ+GD+FLFPKG
Sbjct: 61  VSMAILQYPAGSINPPHSHPRSAELLLVVSGSLQVGFVDTANQFFNQTLQVGDLFLFPKG 120

Query: 142 LVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTSGISDKVLAKAFKTDVTVIESIKA 201
           LVHFQ N DPRNSATAIA F SANPG V+LP +VFTSGISD+VLA+AFKTDV VI+SI+A
Sbjct: 121 LVHFQLNVDPRNSATAIATFASANPGTVALPSTVFTSGISDEVLAEAFKTDVAVIQSIRA 180

Query: 202 GLAPPPS 208
           GL PP S
Sbjct: 181 GLTPPKS 187

[Close]

BLAST of Lag0025812 vs. ExPASy TrEMBL
Match: A0A6J1CLL1 (Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111012724 PE=3 SV=1)

HSP 1 Score: 287.3 bits (734), Expect = 4.9e-74
Identity = 148/208 (71.15%), Postives = 170/208 (81.73%), Query Frame = 0


Query: 4   LSTKSATLFFFFILAI-----FSMAEAGDPDLIVDFLNPPDATLNSTFFTFTGLRSAFAA 63
           ++  S+   FF ++A+     ++MA+AGD DLIVDF+ P D   N +FFTFTGLR A A 
Sbjct: 1   MAMASSYFLFFVVVAMILSSCWNMADAGDADLIVDFIGPLDGGANGSFFTFTGLRFAVAG 60

Query: 64  -FPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGF 123
            FPPNFKPTKATF EFPALLGQSV+L VLQ+PAGA+NPPHTHPRSAELL ++ GTLQVGF
Sbjct: 61  EFPPNFKPTKATFLEFPALLGQSVSLTVLQFPAGAVNPPHTHPRSAELLFLLSGTLQVGF 120

Query: 124 VDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTS 183
           VDTTNKLFNQTLQ+GD FLFPKGL+HFQFN+DP NSATAIAAFGSAN G VS+PLSVF+S
Sbjct: 121 VDTTNKLFNQTLQVGDXFLFPKGLLHFQFNSDPLNSATAIAAFGSANAGTVSVPLSVFSS 180

Query: 184 GISDKVLAKAFKTDVTVIESIKAGLAPP 206
           GISD VLAKAF T V V+  IK GL PP
Sbjct: 181 GISDDVLAKAFNTHVAVVRRIKHGLTPP 208

[Close]

BLAST of Lag0025812 vs. ExPASy TrEMBL
Match: A0A200QHW9 (Germin-like protein OS=Macleaya cordata OX=56857 GN=BVC80_1751g194 PE=3 SV=1)

HSP 1 Score: 255.0 bits (650), Expect = 2.7e-64
Identity = 121/187 (64.71%), Postives = 153/187 (81.82%), Query Frame = 0


Query: 22  MAEAGDPDLIVDFLNPPDA-TLNSTFFTFTGLRSAFAAFPP-NFKPTKATFTEFPALLGQ 81
           + EA DPD++VDF+ PP+   +N T+FTFTG+R+ F   PP NFK TKATF EFPAL GQ
Sbjct: 22  IGEASDPDIVVDFIVPPNTNAVNGTYFTFTGMRTIFETEPPANFKVTKATFAEFPALFGQ 81

Query: 82  SVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDTTNKLFNQTLQLGDVFLFPK 141
           SV+LAVLQ+PAG +NPPHTHPR+AELL V+GG+L+VGF+DTTNKL+ QTLQLGD+F+FPK
Sbjct: 82  SVSLAVLQFPAGGVNPPHTHPRAAELLFVVGGSLEVGFIDTTNKLYTQTLQLGDMFVFPK 141

Query: 142 GLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTSGISDKVLAKAFKTDVTVIESIK 201
           GL H+Q+N++    A A++AFGSAN G VS+P +VF +GI D +LAK+FKTDV  I+ IK
Sbjct: 142 GLAHYQYNSNATEPAIAVSAFGSANAGTVSVPATVFATGIDDGILAKSFKTDVATIQKIK 201

Query: 202 AGLAPPP 207
           AGLAP P
Sbjct: 202 AGLAPKP 208

[Close]

BLAST of Lag0025812 vs. TAIR 10
Match: AT3G62020.1 (germin-like protein 10 )

HSP 1 Score: 126.3 bits (316), Expect = 2.8e-29
Identity = 77/210 (36.67%), Postives = 113/210 (53.81%), Query Frame = 0


Query: 12  FFFFILAIFSMAEAGDPDLIVD--------------FLNPPDATLNSTFFTFTGL-RSAF 71
           FFF +L++  +  A DPD + D              F   P++ + ++ F F G+ + A 
Sbjct: 8   FFFTLLSLNVIVLAYDPDTLQDLCVADRTSGIKVNGFTCKPESNITASDFFFAGIGKPAV 67

Query: 72  AAFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVG 131
                    T A   +   L    V+LA + Y  G LNPPHTHPR+ E++ V+ G L VG
Sbjct: 68  VNNTVGSAVTGANVEKIAGLNTLGVSLARIDYAPGGLNPPHTHPRATEVIFVLEGELDVG 127

Query: 132 FVDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFT 191
           F+ T NKLF +T++ G+VF+FP+GL+H+Q N D    A+ I+AF S  PG  S+  ++FT
Sbjct: 128 FITTANKLFAKTVKKGEVFVFPRGLIHYQKNNDKAKPASVISAFNSQLPGTQSIAATLFT 187

Query: 192 S--GISDKVLAKAFKTDVTVIESIKAGLAP 205
           +   I D VL   F+     IE IK+  AP
Sbjct: 188 ATPAIPDHVLTTTFQIGTKEIEKIKSKFAP 217

[Close]

BLAST of Lag0025812 vs. TAIR 10
Match: AT3G62020.2 (germin-like protein 10 )

HSP 1 Score: 121.7 bits (304), Expect = 6.9e-28
Identity = 68/174 (39.08%), Postives = 99/174 (56.90%), Query Frame = 0


Query: 34  FLNPPDATLNSTFFTFTGL-RSAFAAFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGA 93
           F   P++ + ++ F F G+ + A          T A   +   L    V+LA + Y  G 
Sbjct: 15  FTCKPESNITASDFFFAGIGKPAVVNNTVGSAVTGANVEKIAGLNTLGVSLARIDYAPGG 74

Query: 94  LNPPHTHPRSAELLLVIGGTLQVGFVDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRN 153
           LNPPHTHPR+ E++ V+ G L VGF+ T NKLF +T++ G+VF+FP+GL+H+Q N D   
Sbjct: 75  LNPPHTHPRATEVIFVLEGELDVGFITTANKLFAKTVKKGEVFVFPRGLIHYQKNNDKAK 134

Query: 154 SATAIAAFGSANPGAVSLPLSVFTS--GISDKVLAKAFKTDVTVIESIKAGLAP 205
            A+ I+AF S  PG  S+  ++FT+   I D VL   F+     IE IK+  AP
Sbjct: 135 PASVISAFNSQLPGTQSIAATLFTATPAIPDHVLTTTFQIGTKEIEKIKSKFAP 188

[Close]

BLAST of Lag0025812 vs. TAIR 10
Match: AT1G09560.1 (germin-like protein 5 )

HSP 1 Score: 119.4 bits (298), Expect = 3.4e-27
Identity = 82/223 (36.77%), Postives = 115/223 (51.57%), Query Frame = 0


Query: 4   LSTKSATLFFFFILAIFSMAEAGDPDLIVD--------------FLNPPDATLNSTFFTF 63
           +++ + TL        F ++ + DPD++ D              F     AT+ S  F  
Sbjct: 1   MASPTLTLLLLLTTVSFFISSSADPDMLQDLCVADLPSGIKINGFPCKDAATVTSADFFS 60

Query: 64  TGL------RSAFAAFPPNFKPTKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSA 123
            GL       + F A       T A     P L    V+L+ + Y  G LNPPHTHPR+ 
Sbjct: 61  QGLAKPGLTNNTFGAL-----VTGANVMTIPGLNTLGVSLSRIDYAPGGLNPPHTHPRAT 120

Query: 124 ELLLVIGGTLQVGFVDTTNKLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSA 183
           E++ V+ GTL VGF+ T NKL +Q+L+ GDVF FPKGLVHFQ N +    A+ IAAF S 
Sbjct: 121 EVVFVLEGTLDVGFLTTANKLISQSLKKGDVFAFPKGLVHFQKN-NGDVPASVIAAFNSQ 180

Query: 184 NPGAVSLPLSVF--TSGISDKVLAKAFKTDVTVIESIKAGLAP 205
            PG  SL  ++F  T  + D +LA+AF+T    ++ IK+   P
Sbjct: 181 LPGTQSLGATLFGSTPPVPDNILAQAFQTSPGTVKHIKSKFQP 217

[Close]

BLAST of Lag0025812 vs. TAIR 10
Match: AT1G02335.1 (germin-like protein subfamily 2 member 2 precursor )

HSP 1 Score: 118.6 bits (296), Expect = 5.8e-27
Identity = 76/196 (38.78%), Postives = 111/196 (56.63%), Query Frame = 0


Query: 25  AGDPDLIVDF----------LN--PPDATLNST--FFTFTGL-RSAFAAFPPNFKPTKAT 84
           A DPD + D           LN  P   TLN T   F F G+ + A          T A 
Sbjct: 22  AYDPDALQDLCVADKSHGTKLNGFPCKETLNITESDFFFAGISKPAVINSTMGSAVTGAN 81

Query: 85  FTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDTTNKLFNQTL 144
             + P L   SV+LA + Y  G LNPPHTHPR+ E++ V+ G L+VGF+ T NKLF +T+
Sbjct: 82  VEKIPGLNTLSVSLARIDYAPGGLNPPHTHPRATEVVYVLEGELEVGFITTANKLFTKTI 141

Query: 145 QLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTS--GISDKVLAKA 204
           ++G+VF+FP+GLVHFQ N + ++ A+ ++AF S  PG  S+  ++F +   + + VL K 
Sbjct: 142 KIGEVFVFPRGLVHFQKN-NGKSPASVLSAFNSQLPGTASVAATLFAAEPALPEDVLTKT 201

[Close]

BLAST of Lag0025812 vs. TAIR 10
Match: AT3G05950.1 (RmlC-like cupins superfamily protein )

HSP 1 Score: 113.6 bits (283), Expect = 1.9e-25
Identity = 61/140 (43.57%), Postives = 88/140 (62.86%), Query Frame = 0


Query: 66  TKATFTEFPALLGQSVALAVLQYPAGALNPPHTHPRSAELLLVIGGTLQVGFVDT---TN 125
           T     + P L    V+L  + +  G  NPPHTHPR+ E+L+V+ GTL VGFV +    N
Sbjct: 81  TNVNVDKIPGLNTLGVSLVRIDFAPGGQNPPHTHPRATEILVVVEGTLLVGFVTSNQDNN 140

Query: 126 KLFNQTLQLGDVFLFPKGLVHFQFNADPRNSATAIAAFGSANPGAVSLPLSVFTS--GIS 185
           +LF++ L  GDVF+FP G++HFQ N   R +A A A  GS NPG +++  +VF S   I 
Sbjct: 141 RLFSKVLYPGDVFVFPIGMIHFQVNVG-RTNAVAFAGLGSQNPGTITIADAVFGSKPSIM 200

Query: 186 DKVLAKAFKTDVTVIESIKA 201
            ++LAKAF+ DV V++ ++A
Sbjct: 201 PEILAKAFQLDVNVVKYLEA 219

The following BLAST results are available for this feature:

BLAST of Lag0025812 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038893494.1	1.9e-83	77.40	putative germin-like protein 9-2 [Benincasa hispida]	[more]
XP_008463986.1	4.1e-83	76.67	PREDICTED: germin-like protein 9-3 [Cucumis melo] >KAA0035508.1 germin-like prot...	[more]
XP_031736138.1	5.0e-81	75.47	putative germin-like protein 9-2 [Cucumis sativus]	[more]
XP_022142670.1	1.0e-73	71.15	putative germin-like protein 9-2 [Momordica charantia]	[more]
XP_004139583.2	1.5e-72	75.27	putative germin-like protein 9-2 [Cucumis sativus]	[more]

BLAST of Lag0025812 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q652Q0	3.9e-52	55.21	Putative germin-like protein 9-2 OS=Oryza sativa subsp. japonica OX=39947 GN=Os0...	[more]
Q652P9	1.1e-51	52.61	Germin-like protein 9-3 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568700...	[more]
Q652Q1	3.9e-44	46.19	Germin-like protein 9-1 OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0568500...	[more]
Q7F731	3.0e-28	38.50	Germin-like protein 1-1 OS=Oryza sativa subsp. japonica OX=39947 GN=GER4 PE=2 SV...	[more]
Q9M263	3.9e-28	36.67	Germin-like protein subfamily 2 member 4 OS=Arabidopsis thaliana OX=3702 GN=GLP1...	[more]

BLAST of Lag0025812 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SY36	2.0e-83	76.67	Germin-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G...	[more]
A0A1S3CKF8	2.0e-83	76.67	Germin-like protein OS=Cucumis melo OX=3656 GN=LOC103501975 PE=3 SV=1	[more]
A0A0A0LSQ3	5.8e-75	75.40	Germin-like protein OS=Cucumis sativus OX=3659 GN=Csa_1G166250 PE=3 SV=1	[more]
A0A6J1CLL1	4.9e-74	71.15	Germin-like protein OS=Momordica charantia OX=3673 GN=LOC111012724 PE=3 SV=1	[more]
A0A200QHW9	2.7e-64	64.71	Germin-like protein OS=Macleaya cordata OX=56857 GN=BVC80_1751g194 PE=3 SV=1	[more]

BLAST of Lag0025812 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62020.1	2.8e-29	36.67	germin-like protein 10	[more]
AT3G62020.2	6.9e-28	39.08	germin-like protein 10	[more]
AT1G09560.1	3.4e-27	36.77	germin-like protein 5	[more]
AT1G02335.1	5.8e-27	38.78	germin-like protein subfamily 2 member 2 precursor	[more]
AT3G05950.1	1.9e-25	43.57	RmlC-like cupins superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001929	Germin	PRINTS	PR00325	GERMIN	coord: 97..117 score: 55.37 coord: 161..176 score: 36.34 coord: 127..147 score: 57.03
IPR006045	Cupin 1	SMART	SM00835	Cupin_1_3	coord: 61..196 e-value: 7.1E-33 score: 125.2
IPR006045	Cupin 1	PFAM	PF00190	Cupin_1	coord: 67..193 e-value: 1.2E-29 score: 102.9
IPR014710	RmlC-like jelly roll fold	GENE3D	2.60.120.10	Jelly Rolls	coord: 26..203 e-value: 1.0E-52 score: 180.5
None	No IPR available	PANTHER	PTHR31238	GERMIN-LIKE PROTEIN SUBFAMILY 3 MEMBER 3	coord: 8..200
None	No IPR available	PANTHER	PTHR31238:SF181	GERMIN-LIKE PROTEIN	coord: 8..200
None	No IPR available	CDD	cd02241	cupin_OxOx	coord: 23..202 e-value: 2.48064E-58 score: 180.099
IPR011051	RmlC-like cupin domain superfamily	SUPERFAMILY	51182	RmlC-like cupins	coord: 68..201

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0025812.1	Lag0025812.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0048046	apoplast
molecular_function	GO:0030145	manganese ion binding