Lag0024585 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0024585 vs. NCBI nr
Match: XP_022137103.1 (GDSL esterase/lipase At3g48460 [Momordica charantia])

HSP 1 Score: 694.1 bits (1790), Expect = 6.6e-196
Identity = 330/375 (88.00%), Postives = 348/375 (92.80%), Query Frame = 0

Query: 3   ALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSS 62
           +LT Y+A+++LLC  A  S TATET+PRLF+KIYAFGDSFTDTGNTRS+SGPTGFGHVSS
Sbjct: 5   SLTYYTAVYVLLCAFASASPTATETHPRLFNKIYAFGDSFTDTGNTRSMSGPTGFGHVSS 64

Query: 63  PPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTAIN 122
           PPYGSTFFH  TNRYSDGRLVIDFVA+TLSLPFLPPYKYL GNDSFHGVNFAVAGSTAIN
Sbjct: 65  PPYGSTFFHHPTNRYSDGRLVIDFVAETLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAIN 124

Query: 123 HDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVN 182
           HDFFVRNNLSLDITPQSIQTQLLWFNEFLET+GCRGAETE QCKAA DDALFW GEIGVN
Sbjct: 125 HDFFVRNNLSLDITPQSIQTQLLWFNEFLETQGCRGAETEKQCKAALDDALFWVGEIGVN 184

Query: 183 DYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPVND 242
           DYAYDIGSPIPDDTIRKLGVASVTG LQSLLKKGAKYVVVQGLPPSGCL LAM+LAPV D
Sbjct: 185 DYAYDIGSPIPDDTIRKLGVASVTGFLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPVTD 244

Query: 243 RDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGFRE 302
           RDDIGCVRS +NQTY HN ALQASLQSLR QFPQAVIIYADYWNAYRTV+KNPS YGF+E
Sbjct: 245 RDDIGCVRSVSNQTYVHNVALQASLQSLRGQFPQAVIIYADYWNAYRTVIKNPSNYGFKE 304

Query: 303 RFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGGFT 362
           RFKACCG+GEPYNFD+F VCGMSS SSCKNPSEYINWDGVHLTEAMYK+VHDMFIEGG T
Sbjct: 305 RFKACCGVGEPYNFDVFTVCGMSSVSSCKNPSEYINWDGVHLTEAMYKIVHDMFIEGGLT 364

Query: 363 HPPFSNLLNMKRYEG 378
            PPFS LL+MKR  G
Sbjct: 365 RPPFSYLLDMKRRLG 379

BLAST of Lag0024585 vs. NCBI nr
Match: XP_038893592.1 (GDSL esterase/lipase At3g48460 [Benincasa hispida])

HSP 1 Score: 692.6 bits (1786), Expect = 1.9e-195
Identity = 332/377 (88.06%), Postives = 352/377 (93.37%), Query Frame = 0

Query: 1   MVALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHV 60
           MVALTS SAIFILLC  A  S TATET+P LF+KIYAFGDSFTDTGNTRSVSGPTGFGHV
Sbjct: 2   MVALTSCSAIFILLCFFASASPTATETHPHLFNKIYAFGDSFTDTGNTRSVSGPTGFGHV 61

Query: 61  SSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTA 120
           SSPPYGSTFFH STNRYSDGRLVIDFVAQ+LSLPFLPPYKYL GNDSFHGVNFAVAGSTA
Sbjct: 62  SSPPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTA 121

Query: 121 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 180
           INH+FFVRNNLSLDITPQSIQTQLLWFN+FLET+GCRG ET+ QCKAAFD+ALFW GEIG
Sbjct: 122 INHEFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGEETKPQCKAAFDNALFWVGEIG 181

Query: 181 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 240
           VNDYAY IGS IP+DTIRKLGVASVTGVLQ LLKKGAKYVVVQGLPPSGCL L+M+LAPV
Sbjct: 182 VNDYAYSIGSSIPEDTIRKLGVASVTGVLQFLLKKGAKYVVVQGLPPSGCLALSMSLAPV 241

Query: 241 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 300
           +DRD+IGCV S NNQTY HN ALQASLQSLR QFPQAVIIYADYWNAYR+VMKNPS+YGF
Sbjct: 242 DDRDNIGCVGSLNNQTYVHNIALQASLQSLRGQFPQAVIIYADYWNAYRSVMKNPSQYGF 301

Query: 301 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 360
           RERFKACCG+GEPYNFD+F VCGMSS  SCKNPSEYINWDGVHLTEAMYKVVHDM IEGG
Sbjct: 302 RERFKACCGVGEPYNFDIFTVCGMSSVGSCKNPSEYINWDGVHLTEAMYKVVHDMLIEGG 361

Query: 361 FTHPPFSNLLNMKRYEG 378
           FTHPPFSNLL+MKR++G
Sbjct: 362 FTHPPFSNLLDMKRHQG 378

BLAST of Lag0024585 vs. NCBI nr
Match: XP_022923808.1 (GDSL esterase/lipase At3g48460 [Cucurbita moschata])

HSP 1 Score: 682.9 bits (1761), Expect = 1.5e-192
Identity = 329/375 (87.73%), Postives = 344/375 (91.73%), Query Frame = 0

Query: 1   MVALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHV 60
           MVALTS +AIFILLC   L S TATET+PRLF+KIYAFGDSFTDTGNTRSVSGP GFGHV
Sbjct: 2   MVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHV 61

Query: 61  SSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTA 120
           S+ PYGSTFFH STNRYSDGRLVIDFVAQ+LSLPFLPPYKYL GNDSFHGVNFAVAGSTA
Sbjct: 62  SNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTA 121

Query: 121 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 180
           INH FFVRNNLSLDITPQSIQTQLLWFN+FLET+GCRGAET+TQCKAAFDDALFW GEIG
Sbjct: 122 INHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIG 181

Query: 181 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 240
           VNDYAY +GS I DDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCL LAM+LAPV
Sbjct: 182 VNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAPV 241

Query: 241 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 300
            DRDDIGCVRS NNQTY HN ALQASLQSLR QFPQAVIIYADYWNAYR VMKNPSKYGF
Sbjct: 242 TDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGF 301

Query: 301 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 360
           RERFKACCG+GEPYNFDMF VCGM S SSCKNP EYINWDGVHLTEAMYK VHDMFI+GG
Sbjct: 302 RERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKGG 361

Query: 361 FTHPPFSNLLNMKRY 376
            THP  SNLL+MK +
Sbjct: 362 LTHPALSNLLDMKHH 376

BLAST of Lag0024585 vs. NCBI nr
Match: XP_023520155.1 (GDSL esterase/lipase At3g48460 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 681.0 bits (1756), Expect = 5.8e-192
Identity = 328/375 (87.47%), Postives = 344/375 (91.73%), Query Frame = 0

Query: 1   MVALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHV 60
           MVALTS +AIFILLC   L S TATET+PRLF+KIYAFGDSFTDTGNTRSVSGP GFGHV
Sbjct: 2   MVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHV 61

Query: 61  SSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTA 120
           S+ PYGSTFFH STNRYSDGRLVIDFVAQ+LSLPFLPPYKYL GNDSFHGVNFAVAGSTA
Sbjct: 62  SNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTA 121

Query: 121 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 180
           INH FFVRNNLSLDITPQSIQTQLLWFN+FLET+GCRGAET+TQCKAAFDDALFW GEIG
Sbjct: 122 INHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIG 181

Query: 181 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 240
           VNDYAY +GS I DDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCL LAM+LAPV
Sbjct: 182 VNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPV 241

Query: 241 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 300
            DRDDIGCV+S NNQTY HN ALQASLQSLR QFPQAVIIYADYWNAYR VMKNPSKYGF
Sbjct: 242 TDRDDIGCVKSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGF 301

Query: 301 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 360
           RERFKACCG+GEPYNFDMF VCGM S SSCK+P EYINWDGVHLTEAMYK VHDMFI+GG
Sbjct: 302 RERFKACCGVGEPYNFDMFTVCGMPSVSSCKSPPEYINWDGVHLTEAMYKAVHDMFIKGG 361

Query: 361 FTHPPFSNLLNMKRY 376
            THP  SNLL+MK +
Sbjct: 362 LTHPALSNLLDMKHH 376

BLAST of Lag0024585 vs. NCBI nr
Match: KAG6584128.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 680.6 bits (1755), Expect = 7.5e-192
Identity = 328/375 (87.47%), Postives = 343/375 (91.47%), Query Frame = 0

Query: 1   MVALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHV 60
           MVALTS +AIFILLC   L S TATET+PRLF+KIYAFGDSFTDTGNTRSVSGP GFGHV
Sbjct: 2   MVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHV 61

Query: 61  SSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTA 120
           S+ PYGSTFFH STNRYSDGRLVIDFVAQ+LSLPFLPPYKYL GNDSFHGVNFAVAGSTA
Sbjct: 62  SNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTA 121

Query: 121 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 180
           INH FFVRNNLSLDITPQSIQTQLLWFN+FLET+ CRGAET+TQCKAAFDDALFW GEIG
Sbjct: 122 INHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQACRGAETKTQCKAAFDDALFWVGEIG 181

Query: 181 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 240
           VNDYAY +GS I DDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCL LAM+LAPV
Sbjct: 182 VNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAPV 241

Query: 241 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 300
            DRDDIGCVRS NNQTY HN ALQASLQSLR QFPQAVIIYADYWNAYR VMKNPSKYGF
Sbjct: 242 TDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGF 301

Query: 301 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 360
           RERFKACCG+GEPYNFDMF VCGM S SSCKNP EYINWDGVHLTEAMYK VHDMFI+GG
Sbjct: 302 RERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKGG 361

Query: 361 FTHPPFSNLLNMKRY 376
            THP  SNLL+MK +
Sbjct: 362 LTHPALSNLLDMKHH 376

BLAST of Lag0024585 vs. ExPASy Swiss-Prot
Match: Q9STM6 (GDSL esterase/lipase At3g48460 OS=Arabidopsis thaliana OX=3702 GN=At3g48460 PE=2 SV=1)

HSP 1 Score: 472.2 bits (1214), Expect = 5.3e-132
Identity = 227/373 (60.86%), Postives = 284/373 (76.14%), Query Frame = 0

Query: 8   SAIFILLCVSALVSRTAT-ETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSPPYG 67
           +AI + + + + +S  AT     R F+KIYAFGDSFTDTGN+RS  GP GFGH+SSPPYG
Sbjct: 11  TAISVAILLFSTISTAATIPNIHRPFNKIYAFGDSFTDTGNSRSGEGPAGFGHLSSPPYG 70

Query: 68  STFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPY---KYLNGNDS---FHGVNFAVAGSTA 127
            TFF R TNRYSDGRL IDFVA++++LPFLPPY   K  N N +    HGVNFAV+GST 
Sbjct: 71  MTFFRRPTNRYSDGRLTIDFVAESMNLPFLPPYLSLKTTNANGTATDTHGVNFAVSGSTV 130

Query: 128 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 187
           I H FFV+NNLSLD+TPQSI+T+L WF ++LET G        Q  + F D+LFW GEIG
Sbjct: 131 IKHAFFVKNNLSLDMTPQSIETELAWFEKYLETLG------TNQKVSLFKDSLFWIGEIG 190

Query: 188 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 247
           VNDYAY +GS +  DTIR+L +++ T  L++LL KG KY++VQG P +GCL LAM+LA  
Sbjct: 191 VNDYAYTLGSTVSSDTIRELSISTFTRFLETLLNKGVKYMLVQGHPATGCLTLAMSLAAE 250

Query: 248 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 307
           +DRD +GCV+S NNQ+Y HN ALQ+ L+ LR ++P A I+YADYWNAYR V+K+PSKYG 
Sbjct: 251 DDRDSLGCVQSANNQSYTHNLALQSKLKQLRIKYPSATIVYADYWNAYRAVIKHPSKYGI 310

Query: 308 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 367
            E+FKACCGIGEPYNF +F  CG  +A+ CK+P++YINWDGVHLTEAMYKV+ DMF++G 
Sbjct: 311 TEKFKACCGIGEPYNFQVFQTCGTDAATVCKDPNQYINWDGVHLTEAMYKVMADMFLDGT 370

Query: 368 FTHPPFSNLLNMK 374
           FT P FS+LL  K
Sbjct: 371 FTRPRFSDLLIKK 377

BLAST of Lag0024585 vs. ExPASy Swiss-Prot
Match: Q94F40 (GDSL esterase/lipase At1g28600 OS=Arabidopsis thaliana OX=3702 GN=At1g28600 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 8.7e-66
Identity = 138/371 (37.20%), Postives = 203/371 (54.72%), Query Frame = 0

Query: 4   LTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSP 63
           + S  ++ I L  +  V+  ++ET    F  I +FGDS  DTGN   +S        + P
Sbjct: 1   MASLDSLVIFLFSTLFVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFP 60

Query: 64  PYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTAINH 123
           PYG TFFH  T R  DGR+++DF+A+ + LP++PPY      +   GVNFAVAG+TA+  
Sbjct: 61  PYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALKS 120

Query: 124 DFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVND 183
            F  +  +    T  S+  QL  F + L         + + C+    +AL   GEIG ND
Sbjct: 121 SFLKKRGIQ-PHTNVSLGVQLKSFKKSLPNL----CGSPSDCRDMIGNALILMGEIGGND 180

Query: 184 YAYDIGSPIPDDTIRKL---GVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 243
           Y +   +  P   + +L    +AS++  +  L+  G K  +V G  P GC  + +TL   
Sbjct: 181 YNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKT 240

Query: 244 NDRDD----IGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPS 303
           +++D+     GC++  N    YH+  L+  L  LR+ +P   IIYADY+N+   + K P+
Sbjct: 241 SNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPA 300

Query: 304 KYGFRER-FKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDM 363
           K+GF ER F ACCGIG PYNF+    CG     SCK+PS+Y+ WDGVH+TEA YK + D 
Sbjct: 301 KFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIADG 360

Query: 364 FIEGGFTHPPF 367
            + G + +PPF
Sbjct: 361 ILNGPYANPPF 366

BLAST of Lag0024585 vs. ExPASy Swiss-Prot
Match: Q3E7I6 (GDSL esterase/lipase At1g28650 OS=Arabidopsis thaliana OX=3702 GN=At1g28650 PE=2 SV=1)

HSP 1 Score: 244.2 bits (622), Expect = 2.4e-63
Identity = 139/371 (37.47%), Postives = 203/371 (54.72%), Query Frame = 0

Query: 4   LTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSP 63
           + S    F+L+     +   ++E+  R +  I +FGDS  DTGN   +S        +  
Sbjct: 7   MASLIVSFLLILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFL 66

Query: 64  PYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFH-GVNFAVAGSTAIN 123
           PYG +FFH  + RYSDGRLVIDF+A+ L LP++PPY + + N SF+ G+NFAV G+TA++
Sbjct: 67  PYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPY-FGSQNVSFNQGINFAVYGATALD 126

Query: 124 HDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVN 183
             F V+  +  D T  S+  QL  F + L    C  A +   C+    D+L   GEIG N
Sbjct: 127 RAFLVKQGIKSDFTNISLSVQLNTFKQILPNL-C--ASSTRDCREMLGDSLILMGEIGGN 186

Query: 184 DYAYDIGSPIPDDTIRKLG---VASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTL-- 243
           DY Y        + I++L    + +++  +  L+  G K  +V G  P GC    +TL  
Sbjct: 187 DYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQ 246

Query: 244 -APVNDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPS 303
            A V      GC+   N    +HN  L+  L+ L++ +P   IIYADY+N+   + + P+
Sbjct: 247 TATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPA 306

Query: 304 KYGFRER-FKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDM 363
           KYGF+ R   ACCG+G  YNF +   CG +  S C+NPSEY+NWDG HLTEA Y+ +   
Sbjct: 307 KYGFKNRPLAACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYHLTEATYQKMAQG 366

Query: 364 FIEGGFTHPPF 367
            + G +T P F
Sbjct: 367 LLNGRYTTPAF 373

BLAST of Lag0024585 vs. ExPASy Swiss-Prot
Match: Q8RXT9 (GDSL esterase/lipase At1g28590 OS=Arabidopsis thaliana OX=3702 GN=At1g28590 PE=2 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 3.1e-63
Identity = 134/376 (35.64%), Postives = 206/376 (54.79%), Query Frame = 0

Query: 1   MVALTSYSAIFIL--LCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFG 60
           M +L S  A+ ++  +  + LV+   ++T  R F  I +FGDS  DTGN   +S P    
Sbjct: 1   MASLDSLPAMKLVRFILSTLLVTSVNSQTQCRNFKSIISFGDSIADTGNLLGLSDPNDLP 60

Query: 61  HVSSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGS 120
             + PPYG TFFH  T RYSDGRL+IDF+A+ L  P +PP+      +   GVNFAVAG+
Sbjct: 61  ASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGA 120

Query: 121 TAINHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGE 180
           TA+   F     +   IT  S+  QL  F E L         + + C+   ++AL   GE
Sbjct: 121 TALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNL----CGSPSDCRDMIENALILMGE 180

Query: 181 IGVNDYAYDIGSPIPDDTIRKL---GVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAM 240
           IG NDY + +    P   + +L    +A+++  +  L+  G +  +V G  P G     +
Sbjct: 181 IGGNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYL 240

Query: 241 TLAPVNDRDD----IGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTV 300
           TL   +++++     GC++  N+ + Y+N  LQ  L  LR+ +P   IIYADY+NA   +
Sbjct: 241 TLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRL 300

Query: 301 MKNPSKYGFRER-FKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYK 360
            + P+K+GF  R   ACCG+G  YNF+    CG      C +PS+Y+N+DG+H+TEA Y+
Sbjct: 301 FQEPAKFGFMNRPLPACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYR 360

Query: 361 VVHDMFIEGGFTHPPF 367
           ++ +  ++G +  PPF
Sbjct: 361 LISEGLLKGPYAIPPF 372

BLAST of Lag0024585 vs. ExPASy Swiss-Prot
Match: Q9ZQI3 (GDSL esterase/lipase At2g27360 OS=Arabidopsis thaliana OX=3702 GN=At2g27360 PE=2 SV=1)

HSP 1 Score: 243.0 bits (619), Expect = 5.3e-63
Identity = 131/358 (36.59%), Postives = 196/358 (54.75%), Query Frame = 0

Query: 19  LVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSPPYGSTFFHRSTNRYS 78
           L++   ++T  R F  I +FGDS TDTGN   +S P      + PPYG TFFH  + R+S
Sbjct: 18  LITVVTSQTRCRNFKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETFFHHPSGRFS 77

Query: 79  DGRLVIDFVAQTLSLPFLPPYKYLNGNDSFH-GVNFAVAGSTAINHDFFVRNNLSLDITP 138
           DGRL+IDF+A+ L +P +PP+ Y + N +F  GVNFAV G+TA+              + 
Sbjct: 78  DGRLIIDFIAEFLGIPHVPPF-YGSKNGNFEKGVNFAVGGATALECSVLEEKGTHCSQSN 137

Query: 139 QSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVNDYAYDIGSPIPDDTI 198
            S+  QL  F E L    C    +   C+   ++A    GEIG NDY + +      + +
Sbjct: 138 ISLGNQLKSFKESLPYL-C--GSSSPDCRDMIENAFILIGEIGGNDYNFPLFDRKNIEEV 197

Query: 199 RK---LGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPVNDRDD----IGCVR 258
           ++   L + +++  +  L+  GA+  +V G  P GC    +TL    ++++     GC+ 
Sbjct: 198 KELVPLVITTISSAISELVDMGARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLT 257

Query: 259 SPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGFRER-FKACCG 318
             N+ + YHN  LQA L+ LR  +P   IIY DY+N    +M+ PSK+G  +R   ACCG
Sbjct: 258 WLNDFSVYHNEQLQAELKRLRNLYPHVNIIYGDYYNTLLRLMQEPSKFGLMDRPLPACCG 317

Query: 319 IGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGGFTHPPFS 368
           +G PYNF     CG      C +PS+Y+NWDG+H+TEA YK + +  + G +  PPF+
Sbjct: 318 LGGPYNFTFSIKCGSKGVEYCSDPSKYVNWDGIHMTEAAYKWISEGVLTGPYAIPPFN 371

BLAST of Lag0024585 vs. ExPASy TrEMBL
Match: A0A6J1C9C3 (GDSL esterase/lipase At3g48460 OS=Momordica charantia OX=3673 GN=LOC111008661 PE=3 SV=1)

HSP 1 Score: 694.1 bits (1790), Expect = 3.2e-196
Identity = 330/375 (88.00%), Postives = 348/375 (92.80%), Query Frame = 0

Query: 3   ALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSS 62
           +LT Y+A+++LLC  A  S TATET+PRLF+KIYAFGDSFTDTGNTRS+SGPTGFGHVSS
Sbjct: 5   SLTYYTAVYVLLCAFASASPTATETHPRLFNKIYAFGDSFTDTGNTRSMSGPTGFGHVSS 64

Query: 63  PPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTAIN 122
           PPYGSTFFH  TNRYSDGRLVIDFVA+TLSLPFLPPYKYL GNDSFHGVNFAVAGSTAIN
Sbjct: 65  PPYGSTFFHHPTNRYSDGRLVIDFVAETLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAIN 124

Query: 123 HDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVN 182
           HDFFVRNNLSLDITPQSIQTQLLWFNEFLET+GCRGAETE QCKAA DDALFW GEIGVN
Sbjct: 125 HDFFVRNNLSLDITPQSIQTQLLWFNEFLETQGCRGAETEKQCKAALDDALFWVGEIGVN 184

Query: 183 DYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPVND 242
           DYAYDIGSPIPDDTIRKLGVASVTG LQSLLKKGAKYVVVQGLPPSGCL LAM+LAPV D
Sbjct: 185 DYAYDIGSPIPDDTIRKLGVASVTGFLQSLLKKGAKYVVVQGLPPSGCLALAMSLAPVTD 244

Query: 243 RDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGFRE 302
           RDDIGCVRS +NQTY HN ALQASLQSLR QFPQAVIIYADYWNAYRTV+KNPS YGF+E
Sbjct: 245 RDDIGCVRSVSNQTYVHNVALQASLQSLRGQFPQAVIIYADYWNAYRTVIKNPSNYGFKE 304

Query: 303 RFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGGFT 362
           RFKACCG+GEPYNFD+F VCGMSS SSCKNPSEYINWDGVHLTEAMYK+VHDMFIEGG T
Sbjct: 305 RFKACCGVGEPYNFDVFTVCGMSSVSSCKNPSEYINWDGVHLTEAMYKIVHDMFIEGGLT 364

Query: 363 HPPFSNLLNMKRYEG 378
            PPFS LL+MKR  G
Sbjct: 365 RPPFSYLLDMKRRLG 379

BLAST of Lag0024585 vs. ExPASy TrEMBL
Match: A0A6J1EAM5 (GDSL esterase/lipase At3g48460 OS=Cucurbita moschata OX=3662 GN=LOC111431411 PE=3 SV=1)

HSP 1 Score: 682.9 bits (1761), Expect = 7.3e-193
Identity = 329/375 (87.73%), Postives = 344/375 (91.73%), Query Frame = 0

Query: 1   MVALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHV 60
           MVALTS +AIFILLC   L S TATET+PRLF+KIYAFGDSFTDTGNTRSVSGP GFGHV
Sbjct: 2   MVALTSCTAIFILLCGFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHV 61

Query: 61  SSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTA 120
           S+ PYGSTFFH STNRYSDGRLVIDFVAQ+LSLPFLPPYKYL GNDSFHGVNFAVAGSTA
Sbjct: 62  SNSPYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTA 121

Query: 121 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 180
           INH FFVRNNLSLDITPQSIQTQLLWFN+FLET+GCRGAET+TQCKAAFDDALFW GEIG
Sbjct: 122 INHGFFVRNNLSLDITPQSIQTQLLWFNKFLETQGCRGAETKTQCKAAFDDALFWVGEIG 181

Query: 181 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 240
           VNDYAY +GS I DDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCL LAM+LAPV
Sbjct: 182 VNDYAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAPV 241

Query: 241 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 300
            DRDDIGCVRS NNQTY HN ALQASLQSLR QFPQAVIIYADYWNAYR VMKNPSKYGF
Sbjct: 242 TDRDDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGF 301

Query: 301 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 360
           RERFKACCG+GEPYNFDMF VCGM S SSCKNP EYINWDGVHLTEAMYK VHDMFI+GG
Sbjct: 302 RERFKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKGG 361

Query: 361 FTHPPFSNLLNMKRY 376
            THP  SNLL+MK +
Sbjct: 362 LTHPALSNLLDMKHH 376

BLAST of Lag0024585 vs. ExPASy TrEMBL
Match: A0A6J1KIW4 (GDSL esterase/lipase At3g48460-like OS=Cucurbita maxima OX=3661 GN=LOC111495644 PE=3 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.5e-190
Identity = 324/372 (87.10%), Postives = 339/372 (91.13%), Query Frame = 0

Query: 4   LTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSP 63
           + S +AIFILLC   L S TATET+PRLF+KIYAFGDSFTDTGNTRSVSGP GFGHVS+ 
Sbjct: 3   VASCTAIFILLCSFVLASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPAGFGHVSNS 62

Query: 64  PYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTAINH 123
           PYGSTFFH STNRYSDGRLVIDFVAQ+LSLPFLPPYKYL GNDSFHGVNFAVAGSTAINH
Sbjct: 63  PYGSTFFHHSTNRYSDGRLVIDFVAQSLSLPFLPPYKYLKGNDSFHGVNFAVAGSTAINH 122

Query: 124 DFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVND 183
            FFVRNNLSLDITPQSIQTQLLWFN FLET+GCRGAET+TQCKAAFDDALFW GEIGVND
Sbjct: 123 GFFVRNNLSLDITPQSIQTQLLWFNMFLETQGCRGAETKTQCKAAFDDALFWVGEIGVND 182

Query: 184 YAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPVNDR 243
           YAY +GS I DDTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCL LAM+LAPV DR
Sbjct: 183 YAYAVGSSITDDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALAMSLAPVTDR 242

Query: 244 DDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGFRER 303
           DDIGCVRS NNQTY HN ALQASLQSLR QFPQAVIIYADYWNAYR VMKNPSKYGFRER
Sbjct: 243 DDIGCVRSLNNQTYVHNIALQASLQSLREQFPQAVIIYADYWNAYRMVMKNPSKYGFRER 302

Query: 304 FKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGGFTH 363
           FKACCG+GEPYNFDMF VCGM S SSCKNP EYINWDGVHLTEAMYK VHDMFI+GG TH
Sbjct: 303 FKACCGVGEPYNFDMFTVCGMPSVSSCKNPPEYINWDGVHLTEAMYKAVHDMFIKGGLTH 362

Query: 364 PPFSNLLNMKRY 376
           P  SNLL+MK +
Sbjct: 363 PALSNLLDMKHH 374

BLAST of Lag0024585 vs. ExPASy TrEMBL
Match: A0A0A0LWI9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G050100 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.2e-188
Identity = 318/375 (84.80%), Postives = 344/375 (91.73%), Query Frame = 0

Query: 1   MVALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHV 60
           MV LT  SAIFILL   A  S TATET+PRLF+KIYAFGDSFTDTGNTRSVSGP+GFGHV
Sbjct: 32  MVTLTYCSAIFILLFAFASASPTATETHPRLFNKIYAFGDSFTDTGNTRSVSGPSGFGHV 91

Query: 61  SSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTA 120
           S+PPYGSTFFH  TNRYSDGRLVIDFVAQ+LSLP LPPY+YL GNDSFHGVNFAVAGSTA
Sbjct: 92  SNPPYGSTFFHHPTNRYSDGRLVIDFVAQSLSLPLLPPYRYLKGNDSFHGVNFAVAGSTA 151

Query: 121 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 180
           INH+F+VRNNLS+DITPQSIQTQLLWFN+FLET+GCRG ET+ QC+AAFDDAL W GEIG
Sbjct: 152 INHEFYVRNNLSIDITPQSIQTQLLWFNKFLETQGCRGEETKAQCEAAFDDALLWVGEIG 211

Query: 181 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 240
           VNDYAY  GSPI  DTIRKLGVASVTGVLQSLLKKGAKY+VVQGLPPSGCL L+M+LA V
Sbjct: 212 VNDYAYSFGSPISPDTIRKLGVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALSMSLASV 271

Query: 241 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 300
           +DRDDIGCVRS NNQTY H+ ALQASLQSLRRQFP+AVIIYADYWNAYRTV+KNP+KYGF
Sbjct: 272 DDRDDIGCVRSLNNQTYVHSMALQASLQSLRRQFPEAVIIYADYWNAYRTVIKNPNKYGF 331

Query: 301 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 360
            ERFKACCG+GEPYNF++F VCGMSS SSCK PSEYINWDGVHLTEAMYKVVHDM IEGG
Sbjct: 332 SERFKACCGVGEPYNFELFTVCGMSSVSSCKTPSEYINWDGVHLTEAMYKVVHDMLIEGG 391

Query: 361 FTHPPFSNLLNMKRY 376
           FTHPPFSNLL+MKR+
Sbjct: 392 FTHPPFSNLLDMKRH 406

BLAST of Lag0024585 vs. ExPASy TrEMBL
Match: A0A1S3AYE5 (GDSL esterase/lipase At3g48460 OS=Cucumis melo OX=3656 GN=LOC103484109 PE=3 SV=1)

HSP 1 Score: 667.5 bits (1721), Expect = 3.2e-188
Identity = 320/377 (84.88%), Postives = 342/377 (90.72%), Query Frame = 0

Query: 1   MVALTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHV 60
           MVALT  SAIFILL   A  S TA ET+PRLF+KIYAFGDSFTDTGNTRS SGP GFGHV
Sbjct: 2   MVALTYCSAIFILLFAFASASPTAIETHPRLFNKIYAFGDSFTDTGNTRSASGPAGFGHV 61

Query: 61  SSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTA 120
           S PPYGSTFFH  TNRYSDGRLVIDFVAQ+LSLP LPPYKYL GNDSFHGVNFAVAGSTA
Sbjct: 62  SDPPYGSTFFHHPTNRYSDGRLVIDFVAQSLSLPLLPPYKYLKGNDSFHGVNFAVAGSTA 121

Query: 121 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 180
           INH+F+VRNNLS+D TPQSIQTQLLWFN+FLET+GCRG ET+ QCKAAFDDALFW GEIG
Sbjct: 122 INHEFYVRNNLSIDNTPQSIQTQLLWFNKFLETQGCRGEETKAQCKAAFDDALFWVGEIG 181

Query: 181 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 240
           VNDYAY  GSPI  DTIRKL VASVTGVLQSLLKKGAKY+VVQGLPPSGCL L+M+LA V
Sbjct: 182 VNDYAYSFGSPISADTIRKLAVASVTGVLQSLLKKGAKYMVVQGLPPSGCLALSMSLASV 241

Query: 241 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 300
           +DRDDIGCVRS NNQTY H+ ALQASLQSLRRQFP+AVIIYADYWNAYRTV+KNPSKYGF
Sbjct: 242 DDRDDIGCVRSLNNQTYVHSMALQASLQSLRRQFPEAVIIYADYWNAYRTVIKNPSKYGF 301

Query: 301 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 360
           RERFKACCG+GEPYNF++F VCGMSS SSCK PSEYINWDGVHLTEAMYKVVHDM IEGG
Sbjct: 302 RERFKACCGVGEPYNFELFTVCGMSSVSSCKTPSEYINWDGVHLTEAMYKVVHDMLIEGG 361

Query: 361 FTHPPFSNLLNMKRYEG 378
           FTHPPFS LL+MKR++G
Sbjct: 362 FTHPPFSYLLDMKRHKG 378

BLAST of Lag0024585 vs. TAIR 10
Match: AT3G48460.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 472.2 bits (1214), Expect = 3.8e-133
Identity = 227/373 (60.86%), Postives = 284/373 (76.14%), Query Frame = 0

Query: 8   SAIFILLCVSALVSRTAT-ETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSPPYG 67
           +AI + + + + +S  AT     R F+KIYAFGDSFTDTGN+RS  GP GFGH+SSPPYG
Sbjct: 11  TAISVAILLFSTISTAATIPNIHRPFNKIYAFGDSFTDTGNSRSGEGPAGFGHLSSPPYG 70

Query: 68  STFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPY---KYLNGNDS---FHGVNFAVAGSTA 127
            TFF R TNRYSDGRL IDFVA++++LPFLPPY   K  N N +    HGVNFAV+GST 
Sbjct: 71  MTFFRRPTNRYSDGRLTIDFVAESMNLPFLPPYLSLKTTNANGTATDTHGVNFAVSGSTV 130

Query: 128 INHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIG 187
           I H FFV+NNLSLD+TPQSI+T+L WF ++LET G        Q  + F D+LFW GEIG
Sbjct: 131 IKHAFFVKNNLSLDMTPQSIETELAWFEKYLETLG------TNQKVSLFKDSLFWIGEIG 190

Query: 188 VNDYAYDIGSPIPDDTIRKLGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 247
           VNDYAY +GS +  DTIR+L +++ T  L++LL KG KY++VQG P +GCL LAM+LA  
Sbjct: 191 VNDYAYTLGSTVSSDTIRELSISTFTRFLETLLNKGVKYMLVQGHPATGCLTLAMSLAAE 250

Query: 248 NDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGF 307
           +DRD +GCV+S NNQ+Y HN ALQ+ L+ LR ++P A I+YADYWNAYR V+K+PSKYG 
Sbjct: 251 DDRDSLGCVQSANNQSYTHNLALQSKLKQLRIKYPSATIVYADYWNAYRAVIKHPSKYGI 310

Query: 308 RERFKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGG 367
            E+FKACCGIGEPYNF +F  CG  +A+ CK+P++YINWDGVHLTEAMYKV+ DMF++G 
Sbjct: 311 TEKFKACCGIGEPYNFQVFQTCGTDAATVCKDPNQYINWDGVHLTEAMYKVMADMFLDGT 370

Query: 368 FTHPPFSNLLNMK 374
           FT P FS+LL  K
Sbjct: 371 FTRPRFSDLLIKK 377

BLAST of Lag0024585 vs. TAIR 10
Match: AT1G28600.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 252.3 bits (643), Expect = 6.2e-67
Identity = 138/371 (37.20%), Postives = 203/371 (54.72%), Query Frame = 0

Query: 4   LTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSP 63
           + S  ++ I L  +  V+  ++ET    F  I +FGDS  DTGN   +S        + P
Sbjct: 1   MASLDSLVIFLFSTLFVTIVSSETPCPNFKSIISFGDSIADTGNLVGLSDRNQLPVTAFP 60

Query: 64  PYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGSTAINH 123
           PYG TFFH  T R  DGR+++DF+A+ + LP++PPY      +   GVNFAVAG+TA+  
Sbjct: 61  PYGETFFHHPTGRSCDGRIIMDFIAEFVGLPYVPPYFGSKNRNFDKGVNFAVAGATALKS 120

Query: 124 DFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVND 183
            F  +  +    T  S+  QL  F + L         + + C+    +AL   GEIG ND
Sbjct: 121 SFLKKRGIQ-PHTNVSLGVQLKSFKKSLPNL----CGSPSDCRDMIGNALILMGEIGGND 180

Query: 184 YAYDIGSPIPDDTIRKL---GVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPV 243
           Y +   +  P   + +L    +AS++  +  L+  G K  +V G  P GC  + +TL   
Sbjct: 181 YNFPFFNRKPVKEVEELVPFVIASISSTITELIGMGGKTFLVPGEFPIGCSVVYLTLYKT 240

Query: 244 NDRDD----IGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPS 303
           +++D+     GC++  N    YH+  L+  L  LR+ +P   IIYADY+N+   + K P+
Sbjct: 241 SNKDEYDPSTGCLKWLNKFGEYHSEKLKVELNRLRKLYPHVNIIYADYYNSLLRIFKEPA 300

Query: 304 KYGFRER-FKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDM 363
           K+GF ER F ACCGIG PYNF+    CG     SCK+PS+Y+ WDGVH+TEA YK + D 
Sbjct: 301 KFGFMERPFPACCGIGGPYNFNFTRKCGSVGVKSCKDPSKYVGWDGVHMTEAAYKWIADG 360

Query: 364 FIEGGFTHPPF 367
            + G + +PPF
Sbjct: 361 ILNGPYANPPF 366

BLAST of Lag0024585 vs. TAIR 10
Match: AT1G28650.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 244.2 bits (622), Expect = 1.7e-64
Identity = 139/371 (37.47%), Postives = 203/371 (54.72%), Query Frame = 0

Query: 4   LTSYSAIFILLCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSP 63
           + S    F+L+     +   ++E+  R +  I +FGDS  DTGN   +S        +  
Sbjct: 7   MASLIVSFLLILYYTTIVVASSESRCRRYKSIISFGDSIADTGNYVHLSNVNNLPQAAFL 66

Query: 64  PYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFH-GVNFAVAGSTAIN 123
           PYG +FFH  + RYSDGRLVIDF+A+ L LP++PPY + + N SF+ G+NFAV G+TA++
Sbjct: 67  PYGESFFHPPSGRYSDGRLVIDFIAEFLGLPYVPPY-FGSQNVSFNQGINFAVYGATALD 126

Query: 124 HDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVN 183
             F V+  +  D T  S+  QL  F + L    C  A +   C+    D+L   GEIG N
Sbjct: 127 RAFLVKQGIKSDFTNISLSVQLNTFKQILPNL-C--ASSTRDCREMLGDSLILMGEIGGN 186

Query: 184 DYAYDIGSPIPDDTIRKLG---VASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTL-- 243
           DY Y        + I++L    + +++  +  L+  G K  +V G  P GC    +TL  
Sbjct: 187 DYNYPFFEGKSINEIKELVPLIIKAISSAIVDLIDLGGKTFLVPGNFPIGCSTAYLTLFQ 246

Query: 244 -APVNDRDDIGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPS 303
            A V      GC+   N    +HN  L+  L+ L++ +P   IIYADY+N+   + + P+
Sbjct: 247 TATVEHDPFTGCIPWLNKFGEHHNEQLKIELKQLQKLYPHVNIIYADYYNSLYGLFQEPA 306

Query: 304 KYGFRER-FKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDM 363
           KYGF+ R   ACCG+G  YNF +   CG +  S C+NPSEY+NWDG HLTEA Y+ +   
Sbjct: 307 KYGFKNRPLAACCGVGGQYNFTIGKECGENGVSYCQNPSEYVNWDGYHLTEATYQKMAQG 366

Query: 364 FIEGGFTHPPF 367
            + G +T P F
Sbjct: 367 LLNGRYTTPAF 373

BLAST of Lag0024585 vs. TAIR 10
Match: AT1G28590.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 243.8 bits (621), Expect = 2.2e-64
Identity = 134/376 (35.64%), Postives = 206/376 (54.79%), Query Frame = 0

Query: 1   MVALTSYSAIFIL--LCVSALVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFG 60
           M +L S  A+ ++  +  + LV+   ++T  R F  I +FGDS  DTGN   +S P    
Sbjct: 1   MASLDSLPAMKLVRFILSTLLVTSVNSQTQCRNFKSIISFGDSIADTGNLLGLSDPNDLP 60

Query: 61  HVSSPPYGSTFFHRSTNRYSDGRLVIDFVAQTLSLPFLPPYKYLNGNDSFHGVNFAVAGS 120
             + PPYG TFFH  T RYSDGRL+IDF+A+ L  P +PP+      +   GVNFAVAG+
Sbjct: 61  ASAFPPYGETFFHHPTGRYSDGRLIIDFIAEFLGFPLVPPFYGCQNANFKKGVNFAVAGA 120

Query: 121 TAINHDFFVRNNLSLDITPQSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGE 180
           TA+   F     +   IT  S+  QL  F E L         + + C+   ++AL   GE
Sbjct: 121 TALEPSFLEERGIHSTITNVSLSVQLRSFTESLPNL----CGSPSDCRDMIENALILMGE 180

Query: 181 IGVNDYAYDIGSPIPDDTIRKL---GVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAM 240
           IG NDY + +    P   + +L    +A+++  +  L+  G +  +V G  P G     +
Sbjct: 181 IGGNDYNFALFQRKPVKEVEELVPFVIATISSAITELVCMGGRTFLVPGNFPIGYSASYL 240

Query: 241 TLAPVNDRDD----IGCVRSPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTV 300
           TL   +++++     GC++  N+ + Y+N  LQ  L  LR+ +P   IIYADY+NA   +
Sbjct: 241 TLYKTSNKEEYDPLTGCLKWLNDFSEYYNKQLQEELNGLRKLYPHVNIIYADYYNALLRL 300

Query: 301 MKNPSKYGFRER-FKACCGIGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYK 360
            + P+K+GF  R   ACCG+G  YNF+    CG      C +PS+Y+N+DG+H+TEA Y+
Sbjct: 301 FQEPAKFGFMNRPLPACCGVGGSYNFNFSRRCGSVGVEYCDDPSQYVNYDGIHMTEAAYR 360

Query: 361 VVHDMFIEGGFTHPPF 367
           ++ +  ++G +  PPF
Sbjct: 361 LISEGLLKGPYAIPPF 372

BLAST of Lag0024585 vs. TAIR 10
Match: AT2G27360.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.7e-64
Identity = 131/358 (36.59%), Postives = 196/358 (54.75%), Query Frame = 0

Query: 19  LVSRTATETYPRLFDKIYAFGDSFTDTGNTRSVSGPTGFGHVSSPPYGSTFFHRSTNRYS 78
           L++   ++T  R F  I +FGDS TDTGN   +S P      + PPYG TFFH  + R+S
Sbjct: 18  LITVVTSQTRCRNFKSIISFGDSITDTGNLLGLSSPNDLPESAFPPYGETFFHHPSGRFS 77

Query: 79  DGRLVIDFVAQTLSLPFLPPYKYLNGNDSFH-GVNFAVAGSTAINHDFFVRNNLSLDITP 138
           DGRL+IDF+A+ L +P +PP+ Y + N +F  GVNFAV G+TA+              + 
Sbjct: 78  DGRLIIDFIAEFLGIPHVPPF-YGSKNGNFEKGVNFAVGGATALECSVLEEKGTHCSQSN 137

Query: 139 QSIQTQLLWFNEFLETKGCRGAETETQCKAAFDDALFWFGEIGVNDYAYDIGSPIPDDTI 198
            S+  QL  F E L    C    +   C+   ++A    GEIG NDY + +      + +
Sbjct: 138 ISLGNQLKSFKESLPYL-C--GSSSPDCRDMIENAFILIGEIGGNDYNFPLFDRKNIEEV 197

Query: 199 RK---LGVASVTGVLQSLLKKGAKYVVVQGLPPSGCLPLAMTLAPVNDRDD----IGCVR 258
           ++   L + +++  +  L+  GA+  +V G  P GC    +TL    ++++     GC+ 
Sbjct: 198 KELVPLVITTISSAISELVDMGARTFLVPGNFPLGCSVAYLTLYETPNKEEYNPLTGCLT 257

Query: 259 SPNNQTYYHNAALQASLQSLRRQFPQAVIIYADYWNAYRTVMKNPSKYGFRER-FKACCG 318
             N+ + YHN  LQA L+ LR  +P   IIY DY+N    +M+ PSK+G  +R   ACCG
Sbjct: 258 WLNDFSVYHNEQLQAELKRLRNLYPHVNIIYGDYYNTLLRLMQEPSKFGLMDRPLPACCG 317

Query: 319 IGEPYNFDMFNVCGMSSASSCKNPSEYINWDGVHLTEAMYKVVHDMFIEGGFTHPPFS 368
           +G PYNF     CG      C +PS+Y+NWDG+H+TEA YK + +  + G +  PPF+
Sbjct: 318 LGGPYNFTFSIKCGSKGVEYCSDPSKYVNWDGIHMTEAAYKWISEGVLTGPYAIPPFN 371