Lag0024272 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0024272
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionS-protein homolog
Locationchr10: 1776738 .. 1777172 (-)
RNA-Seq ExpressionLag0024272
SyntenyLag0024272
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTCTAAACATCTTTTGTTTCATCATATTGCTTGCTTTAGGATCAAACATTATCAAATCATGTTTTGGACAATCCAAGCTACAAGATCCATTGTCCTCATGGCAAGTGACCATATTGAATCATATGAAGGATTCAAGTCTGGTGGCTCATTGCAAGTCGAAAGACGACGATTTGGGCGAGCACATAATCGGTGTCAGAGGTAAATACTACTGGAATTTTGATGAAAATATTTGGCAAACAACATTGTTTTGGTGTAATTTTAGCAGCAAACATGGACAAGCTTCTGGTAATGTTTTCTGGCCAGAGAGTGAAACATTCCTCACTGATAATTGTATTCATGGTAACTGTGTTTGGGCTGCTAGAGACCAAGGCATTTACTTTCATCTTGGCTCTCTCAAGACCTTTCAATTATTGTATCCTTGGAAATGA

mRNA sequence

ATGAAGCTTCTAAACATCTTTTGTTTCATCATATTGCTTGCTTTAGGATCAAACATTATCAAATCATGTTTTGGACAATCCAAGCTACAAGATCCATTGTCCTCATGGCAAGTGACCATATTGAATCATATGAAGGATTCAAGTCTGGTGGCTCATTGCAAGTCGAAAGACGACGATTTGGGCGAGCACATAATCGGTGTCAGAGGTAAATACTACTGGAATTTTGATGAAAATATTTGGCAAACAACATTGTTTTGGTGTAATTTTAGCAGCAAACATGGACAAGCTTCTGGTAATGTTTTCTGGCCAGAGAGTGAAACATTCCTCACTGATAATTGTATTCATGGTAACTGTGTTTGGGCTGCTAGAGACCAAGGCATTTACTTTCATCTTGGCTCTCTCAAGACCTTTCAATTATTGTATCCTTGGAAATGA

Coding sequence (CDS)

ATGAAGCTTCTAAACATCTTTTGTTTCATCATATTGCTTGCTTTAGGATCAAACATTATCAAATCATGTTTTGGACAATCCAAGCTACAAGATCCATTGTCCTCATGGCAAGTGACCATATTGAATCATATGAAGGATTCAAGTCTGGTGGCTCATTGCAAGTCGAAAGACGACGATTTGGGCGAGCACATAATCGGTGTCAGAGGTAAATACTACTGGAATTTTGATGAAAATATTTGGCAAACAACATTGTTTTGGTGTAATTTTAGCAGCAAACATGGACAAGCTTCTGGTAATGTTTTCTGGCCAGAGAGTGAAACATTCCTCACTGATAATTGTATTCATGGTAACTGTGTTTGGGCTGCTAGAGACCAAGGCATTTACTTTCATCTTGGCTCTCTCAAGACCTTTCAATTATTGTATCCTTGGAAATGA

Protein sequence

MKLLNIFCFIILLALGSNIIKSCFGQSKLQDPLSSWQVTILNHMKDSSLVAHCKSKDDDLGEHIIGVRGKYYWNFDENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHGNCVWAARDQGIYFHLGSLKTFQLLYPWK
Homology
BLAST of Lag0024272 vs. NCBI nr
Match: XP_023001658.1 (S-protein homolog 74-like [Cucurbita maxima])

HSP 1 Score: 182.6 bits (462), Expect = 2.5e-42
Identity = 85/148 (57.43%), Postives = 107/148 (72.30%), Query Frame = 0

Query: 1   MKLLNIFCFIILLALGSN-IIKSCFGQ---SKLQDPLSSWQVTILNHMKDSSLVAHCKSK 60
           M+ +N  CFI+LL LGSN ++K CF Q   SK+Q P S WQVTI NHM DS+L+ HCKSK
Sbjct: 1   MESVNPLCFILLLGLGSNMLMKPCFAQPTESKVQLPFSRWQVTIYNHMIDSTLMVHCKSK 60

Query: 61  DDDLGEHIIGVRGKYYWNFDENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHG 120
           D+DLG H I     Y+  F ENIWQTT FWC+FSSK+GQ +G+VFWPE  + L+D C   
Sbjct: 61  DNDLGRHEIKQGDNYFLKFKENIWQTTQFWCHFSSKYGQVTGDVFWPEEGSRLSDECNDH 120

Query: 121 NCVWAARDQGIYFHLGSLKTFQLLYPWK 145
           NCVW+A+D GI+   GS+ ++QL YPWK
Sbjct: 121 NCVWSAQDGGIFLLRGSINSYQLAYPWK 148

BLAST of Lag0024272 vs. NCBI nr
Match: XP_023522257.1 (S-protein homolog 1-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 177.9 bits (450), Expect = 6.1e-41
Identity = 78/115 (67.83%), Postives = 89/115 (77.39%), Query Frame = 0

Query: 19  IIKSCFGQ--SKLQDPLSSWQVTILNHMKDSSLVAHCKSKDDDLGEHIIGVRGKYYWNFD 78
           II SCF Q  SK Q PLS W+V ILNHMKDS+L+AHCKSK+DDLGEH+I   GKYYW F 
Sbjct: 2   IINSCFAQSDSKYQRPLSPWEVAILNHMKDSTLLAHCKSKNDDLGEHVIVPGGKYYWMFS 61

Query: 79  ENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHGNCVWAARDQGIYFHL 132
           EN+WQ+TLFWCNFSSKHGQASG VFWP++  +L D C    C+WAARD GI   L
Sbjct: 62  ENVWQSTLFWCNFSSKHGQASGEVFWPDNGGWLQDRCASNTCIWAARDDGISLRL 116

BLAST of Lag0024272 vs. NCBI nr
Match: XP_022954425.1 (S-protein homolog 74-like [Cucurbita moschata])

HSP 1 Score: 176.0 bits (445), Expect = 2.3e-40
Identity = 75/128 (58.59%), Postives = 92/128 (71.88%), Query Frame = 0

Query: 19  IIKSCFGQ--SKLQDPLSSWQVTILNHMKDSSLVAHCKSKDDDLGEHIIGVRGKYYWNFD 78
           II SCF Q  S L D  S+W+V I+N +KDS+LV HCKSKDDDLGEH+I    KY+W F 
Sbjct: 2   IINSCFAQSDSSLIDQFSNWEVDIVNDLKDSTLVVHCKSKDDDLGEHVIKAGDKYFWKFH 61

Query: 79  ENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHGNCVWAARDQGIYFHLGSLKT 138
           EN+ QTTL+WCNFSSK GQASG VFWPE   +L+D C    C+W A+D GI   LG+   
Sbjct: 62  ENVLQTTLYWCNFSSKRGQASGQVFWPEKSHWLSDRCNRNTCIWVAKDDGISIRLGAFGL 121

Query: 139 FQLLYPWK 145
           ++L+YPWK
Sbjct: 122 YELVYPWK 129

BLAST of Lag0024272 vs. NCBI nr
Match: XP_022954429.1 (S-protein homolog 74-like [Cucurbita moschata])

HSP 1 Score: 175.6 bits (444), Expect = 3.0e-40
Identity = 76/128 (59.38%), Postives = 92/128 (71.88%), Query Frame = 0

Query: 19  IIKSCFGQ--SKLQDPLSSWQVTILNHMKDSSLVAHCKSKDDDLGEHIIGVRGKYYWNFD 78
           II SCF Q  S L D  S+W+V I+N +KDS+LVAHCKSKDDDLGEH+I    KY+W F 
Sbjct: 2   IINSCFAQSDSSLIDQFSNWEVDIVNDLKDSTLVAHCKSKDDDLGEHVIKPGDKYFWKFH 61

Query: 79  ENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHGNCVWAARDQGIYFHLGSLKT 138
           EN+ QTTL+WCNFSSK GQASG VFWPE   +L+D C    C+W A+D GI   LG    
Sbjct: 62  ENVLQTTLYWCNFSSKRGQASGQVFWPEKSHWLSDRCNRNTCIWVAKDDGISIRLGVFGL 121

Query: 139 FQLLYPWK 145
           ++L+YPWK
Sbjct: 122 YELVYPWK 129

BLAST of Lag0024272 vs. NCBI nr
Match: XP_022954427.1 (S-protein homolog 74-like [Cucurbita moschata])

HSP 1 Score: 172.6 bits (436), Expect = 2.5e-39
Identity = 75/128 (58.59%), Postives = 91/128 (71.09%), Query Frame = 0

Query: 19  IIKSCFGQ--SKLQDPLSSWQVTILNHMKDSSLVAHCKSKDDDLGEHIIGVRGKYYWNFD 78
           II SCF Q  S L D  S+W+V I+N +KDS+LVAHCKSKDDDLGEH+I    KY+W F 
Sbjct: 2   IINSCFAQSDSSLIDQFSNWEVDIVNDLKDSTLVAHCKSKDDDLGEHVIKPGDKYFWKFH 61

Query: 79  ENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHGNCVWAARDQGIYFHLGSLKT 138
           EN+ QTTL+WCNFSSK GQA G VFWPE   +L+D C    C+W A+D GI   LG    
Sbjct: 62  ENVLQTTLYWCNFSSKGGQAFGQVFWPEKSHWLSDRCNRNTCIWVAKDDGISIRLGVFGL 121

Query: 139 FQLLYPWK 145
           ++L+YPWK
Sbjct: 122 YELVYPWK 129

BLAST of Lag0024272 vs. ExPASy Swiss-Prot
Match: F4JLS0 (S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 9.8e-18
Identity = 46/132 (34.85%), Postives = 71/132 (53.79%), Query Frame = 0

Query: 2   KLLNIFCFIILLALGSNIIKSCFGQSKLQD----PLSSWQVTILNHM-KDSSLVAHCKSK 61
           + L   CF + L    +++    G +  +D     +S WQVT++N +    +L  HCKSK
Sbjct: 6   QFLLAICFSLALTCQDHVL---VGGTTTRDIIVPKISEWQVTVVNGLTTGETLFIHCKSK 65

Query: 62  DDDLGEHIIGVRGKYYWNFDENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHG 121
           +DDLGE  +  R ++ WNF EN+  +T FWC  +  +G  + NVFW   +  L   C   
Sbjct: 66  EDDLGEINLKFRNRFSWNFGENMLHSTFFWCYMNKDNGHMNVNVFW--DDVILFHRCGWK 125

Query: 122 NCVWAARDQGIY 129
           NC+W A+  G+Y
Sbjct: 126 NCIWTAKTDGLY 132

BLAST of Lag0024272 vs. ExPASy Swiss-Prot
Match: Q2HQ46 (S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.7e-17
Identity = 45/139 (32.37%), Postives = 72/139 (51.80%), Query Frame = 0

Query: 8   CFIILLALGSNII-KSCFGQSKLQDPLSSWQVTILNHM-KDSSLVAHCKSKDDDLGEHII 67
           CF ++L    +++ +    +  +   +S WQVT+ N +    +L  HCKSK++DLG+  +
Sbjct: 12  CFYLVLTCQDHVLARDTTTRDIVVPKISEWQVTVANGLTTGETLFIHCKSKENDLGDINL 71

Query: 68  GVRGKYYWNFDENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHGNCVWAARDQ 127
               ++ WNF EN+  +TLFWC  S   G  +  VFW   +  L   C   NCVW A++ 
Sbjct: 72  KFLDRFSWNFGENMLHSTLFWCYMSKDDGHMNVKVFW--DDVILFHRCDWKNCVWTAKND 131

Query: 128 GIYFHLGSLKTFQLLYPWK 145
           G+Y    ++    L   WK
Sbjct: 132 GLYLWNSAIGEDVLSEKWK 148

BLAST of Lag0024272 vs. ExPASy Swiss-Prot
Match: F2Q9V4 (S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2)

HSP 1 Score: 68.9 bits (167), Expect = 5.2e-11
Identity = 48/139 (34.53%), Postives = 69/139 (49.64%), Query Frame = 0

Query: 6   IFCFIILLAL-GSNIIKSCFGQSKLQDPLSSWQVTILNHMKDSSLVAHCKSKDDDLGEHI 65
           I  FI+L++L G   ++       ++ PL+  +V I N   D  L  HCKS+DDD G HI
Sbjct: 3   IIIFIVLISLIGCETLQHDGKVFPMKGPLT--RVVIYND-NDYLLGVHCKSRDDDHGFHI 62

Query: 66  IGVRGKYYWNFDENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNCIHGNCVWAARD 125
           +   G Y W F  N   +TL++C FS +  Q    VF  +    + D+    NC W A++
Sbjct: 63  LQKGGLYGWMFYVNFMNSTLYFCGFSQE--QVKKGVF--DIYKAVRDSSRCRNCTWEAKE 122

Query: 126 QGIYFHLGSLKTFQLLYPW 144
            GIY +    K   L Y W
Sbjct: 123 DGIYGYGEIPKKNPLFYKW 134

BLAST of Lag0024272 vs. ExPASy Swiss-Prot
Match: O23020 (S-protein homolog 5 OS=Arabidopsis thaliana OX=3702 GN=SPH5 PE=2 SV=1)

HSP 1 Score: 67.4 bits (163), Expect = 1.5e-10
Identity = 35/113 (30.97%), Postives = 59/113 (52.21%), Query Frame = 0

Query: 1   MKLLNIFCFIILLALGSNIIKSCFGQSKLQDPLSSWQVTILNHMKDSSLVAHCKSKDDDL 60
           M+ ++I CF   L  GS       G   L     +  VT+ N +    L  HCKSK DDL
Sbjct: 1   MEKVSIVCFFFFLLFGS-------GYGGLPPFWRATVVTMTNLIGGPPLTIHCKSKQDDL 60

Query: 61  GEHIIGVRGKYYWNFDENIWQTTLFWCNFSSKHGQASGNVFWPESETFLTDNC 114
           G H++  + +Y++ F  N+W++TLF+C+F       S +++  + +  + D+C
Sbjct: 61  GIHVVPFKQEYHFKFQPNLWKSTLFFCSFQWDSQFKSFDIYDAQRDQGICDDC 106

BLAST of Lag0024272 vs. ExPASy Swiss-Prot
Match: Q9LW22 (S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 5.7e-10
Identity = 43/146 (29.45%), Postives = 68/146 (46.58%), Query Frame = 0

Query: 1   MKLLNIFCFIILLALGSNIIKSCFGQSKLQDPLSSWQVTILNHMKDSS---LVAHCKSKD 60
           MK L+IF F++ L + S++    +G+           +T+ N +   +   L  HCKSK+
Sbjct: 1   MKNLSIFLFVVGLCMISDV----YGKKS--------TITVKNELNPKNKNILKVHCKSKN 60

Query: 61  DDLGEHIIGVRGKYYWNFDENIWQTTLFWCNF--SSKHGQASGNVFWPESETFLTDNCIH 120
           +D+G   + +     ++F  N W TT FWCN      + +  G   +     F  D   +
Sbjct: 61  NDIGVKYLKIGEVMSFSFKTNFWGTTEFWCNLYKGPDYKRYRGITAYQAIGLFAKDGSSY 120

Query: 121 GNCVWAARDQGIYFHLGSLKTFQLLY 142
               W ARD GIYFH  SL ++   Y
Sbjct: 121 N---WLARDDGIYFHKDSLPSYYKTY 131

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023001658.12.5e-4257.43S-protein homolog 74-like [Cucurbita maxima][more]
XP_023522257.16.1e-4167.83S-protein homolog 1-like, partial [Cucurbita pepo subsp. pepo][more]
XP_022954425.12.3e-4058.59S-protein homolog 74-like [Cucurbita moschata][more]
XP_022954429.13.0e-4059.38S-protein homolog 74-like [Cucurbita moschata][more]
XP_022954427.12.5e-3958.59S-protein homolog 74-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
F4JLS09.8e-1834.85S-protein homolog 1 OS=Arabidopsis thaliana OX=3702 GN=SPH1 PE=2 SV=1[more]
Q2HQ461.7e-1732.37S-protein homolog 74 OS=Arabidopsis thaliana OX=3702 GN=SPH74 PE=2 SV=1[more]
F2Q9V45.2e-1134.53S-protein homolog 6 OS=Arabidopsis thaliana OX=3702 GN=SPH6 PE=3 SV=2[more]
O230201.5e-1030.97S-protein homolog 5 OS=Arabidopsis thaliana OX=3702 GN=SPH5 PE=2 SV=1[more]
Q9LW225.7e-1029.45S-protein homolog 21 OS=Arabidopsis thaliana OX=3702 GN=SPH21 PE=3 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010264Plant self-incompatibility S1PFAMPF05938Self-incomp_S1coord: 37..143
e-value: 1.9E-23
score: 83.0
IPR010264Plant self-incompatibility S1PANTHERPTHR31232FAMILY NOT NAMEDcoord: 29..136
NoneNo IPR availablePANTHERPTHR31232:SF34PUTATIVE-RELATEDcoord: 29..136

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0024272.1Lag0024272.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009987 cellular process