Lag0023244 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0023244
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionmethyl-CpG-binding domain-containing protein 5-like
Locationchr7: 46356058 .. 46358342 (+)
RNA-Seq ExpressionLag0023244
SyntenyLag0023244
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTGCTTCTGGAACTTCCTTTCAAGACCCGACCCGACCCGACTTCCCTTCCTCCGCCTCCGCCTCCGCCTCCCACCACCTCCCCCCGGACCCCCTCCTCTCCGCCGGCTCCTTCATCGACTCCTCCGCCGCCCCATGCGCCACCCGAGATCTTCACCGCAAGAGGCCTGACACCTCCGCCACTCGCCGATCTCCATTCCGCGCCGACCATCCCAACGGTAACAACCTCCCTCCGGATCCTCCTGCTCCCGGAAATGGAGATTCTCCGCCCTCCAAGAAGCTGTCCGCCTCCAGACCCACCGACGCCTCCCCGAAATCCGCCTCGTTGGAGAGGCCTAATTGGCTGCCGCCGGGCTGGGTCTTGGAAGATAGAGTTCGCGCCTCGGGTGCTACAGCTGGAACGGTCGATAAGGTGATGAATTAGATGATTCCTTCTCTTTATGACTTGAATCGTTTTACCGTCTTGAATCTTGAGTTGCTTTCTCTGTTCTGGCGTTTTGGGTTTTGGAAGATCGTGTTGGAAGTGGAGGGGAATCTGTTCTGGTAGTCAATCGACCATGCAGGTTTTAGTCTAGAACGCGAGAGCTACTCAACTTTCTCTCTGCAATTTAATGTTTGACGATTGAACAGGGTTTTACCGTCGCTTTGAATTGTTTTCTTTTCTACTCTACCTGTTCGACTGTTGGTTTAGAATTTTCAACCAGTTATGGATGATTTTTGGTCTTACTGCGTTTGGAACTTCGGATGATAACATGAACCAATCACAAGAAATTCTCATTGTTACTGAAAATGAAATGATCTTTTGTTTTTGCCTCAAGGGGTTGGTTATAGCATTCCCTTCTCAGTTGATCTCTCATCAATTCTGGAAAAAAAAGAGCTATATCAAGTAGTAAAGGATTTAGTAATCACTCTGGATGGGTTATCATCTGAAATCTCTGTCGTATTGATGGGTATTACACTTTTAGTTACCTGTTTGGGAATTGAAAAGTTTCATAGGAGAAAGTGTTAAGGATTTCATTTCATTCTCAGGATCAGACTCTTCCTCTATAAATTTGTTTAGGGAATACTTTTGTTCTTTATTTGGTATAACATTTAAGTATGTTCTGGTCCATGCTTTCGAATAACAAACTCTCTTGGTCCTTTTCCCTGGTTGGTGAACGAGCCTTGACGAACATTGATATAGATTTAGCAAGCTAACTGACCGATTATACGGAATCAATTAGCATAACAGAGTGTGGTTGATTAGGATGCAGACCATTGGAAAAAGGAATAACAAAAACCTCCACTGTTATCCTGATAACCAGTTCATAAATGGTCAGCATCAAATTTGTATGATAGTATTGATTATTTTATCTCTCTGTGCATGTTAGTGAATAGCTTTGGACTCATATTTGTAGATTTCAAATTCTCTTATTGGAATTTTGCAGTTCATATCATAGGATGTGTGACTAATGCTTGGTGCTTGATATATGATGATGCTTCACTAAGAGTCATGTTTGAGATGGATTCTACCTTAATCTTGTATTCATAATTAGCTGTGTTTAAAACCACATTGACATTGTTGATAGACAAAACAAGTGCGAGCAATGAAGTACGGTCACAAGTACCATCTTGCATTTAATTATTGAGAAAGAGTTTTGAGGAGTGTCGTATGGAAATTGCTTATGAATTGAGTTCTTCTTTTTCCTTTTGTTTTTTGTCATGTCGTGCTGAATTAATTGGGAGCATGCTTGGGCCCATTTATATCTTGAGGTTCCCCCTTCTTATCATCCTGTTTTAGATGTATATATAAACAAACAAATAGTAAAGAATGTTAAAAGAATCTAATATATCCCCATGTCCCTTGCAGTACTACTTCGACCCAGTCTCAGGCCGTCGATTCCGGTCTAAGATTGAGGTTCTTTACTTTCTGGAAACAGGAACGTTAAAGAAACGGAAGAAATCATTAGATGGTAATGCGACGGTGAGTCGAAAGCTAACATTCCTTCTTTGGCACCCAATGGAAAATTCAGATCTTCTCATCCATTTTTTCGCTTTTCCTGATTTGCAACTCTGCAGTCTGCTGATGGCTCCGAAGAACCCAAGACCAAGAAATCTTCAAGCAATGCTAAATCTGCCCCTTTAAACTTTGATTTCTTCAAAGTGCCCGAAAAGGTTGAGTGGGTTCTCACAGACCCTTCTCAGGAGGCTTGGACGCCGTTTATCGACAACGAGAAAGTACCTGAATCTACGAAACGCGAATGGGTGGGAGCTTTTCAACTCCTTGGCCGATCTAAAGTTTGA

mRNA sequence

ATGTCTGCTTCTGGAACTTCCTTTCAAGACCCGACCCGACCCGACTTCCCTTCCTCCGCCTCCGCCTCCGCCTCCCACCACCTCCCCCCGGACCCCCTCCTCTCCGCCGGCTCCTTCATCGACTCCTCCGCCGCCCCATGCGCCACCCGAGATCTTCACCGCAAGAGGCCTGACACCTCCGCCACTCGCCGATCTCCATTCCGCGCCGACCATCCCAACGGTAACAACCTCCCTCCGGATCCTCCTGCTCCCGGAAATGGAGATTCTCCGCCCTCCAAGAAGCTGTCCGCCTCCAGACCCACCGACGCCTCCCCGAAATCCGCCTCGTTGGAGAGGCCTAATTGGCTGCCGCCGGGCTGGGTCTTGGAAGATAGAGTTCGCGCCTCGGGTGCTACAGCTGGAACGGTCGATAAGTACTACTTCGACCCAGTCTCAGGCCGTCGATTCCGGTCTAAGATTGAGGTTCTTTACTTTCTGGAAACAGGAACGTTAAAGAAACGGAAGAAATCATTAGATGGTAATGCGACGTCTGCTGATGGCTCCGAAGAACCCAAGACCAAGAAATCTTCAAGCAATGCTAAATCTGCCCCTTTAAACTTTGATTTCTTCAAAGTGCCCGAAAAGGTTGAGTGGGTTCTCACAGACCCTTCTCAGGAGGCTTGGACGCCGTTTATCGACAACGAGAAAGTACCTGAATCTACGAAACGCGAATGGGTGGGAGCTTTTCAACTCCTTGGCCGATCTAAAGTTTGA

Coding sequence (CDS)

ATGTCTGCTTCTGGAACTTCCTTTCAAGACCCGACCCGACCCGACTTCCCTTCCTCCGCCTCCGCCTCCGCCTCCCACCACCTCCCCCCGGACCCCCTCCTCTCCGCCGGCTCCTTCATCGACTCCTCCGCCGCCCCATGCGCCACCCGAGATCTTCACCGCAAGAGGCCTGACACCTCCGCCACTCGCCGATCTCCATTCCGCGCCGACCATCCCAACGGTAACAACCTCCCTCCGGATCCTCCTGCTCCCGGAAATGGAGATTCTCCGCCCTCCAAGAAGCTGTCCGCCTCCAGACCCACCGACGCCTCCCCGAAATCCGCCTCGTTGGAGAGGCCTAATTGGCTGCCGCCGGGCTGGGTCTTGGAAGATAGAGTTCGCGCCTCGGGTGCTACAGCTGGAACGGTCGATAAGTACTACTTCGACCCAGTCTCAGGCCGTCGATTCCGGTCTAAGATTGAGGTTCTTTACTTTCTGGAAACAGGAACGTTAAAGAAACGGAAGAAATCATTAGATGGTAATGCGACGTCTGCTGATGGCTCCGAAGAACCCAAGACCAAGAAATCTTCAAGCAATGCTAAATCTGCCCCTTTAAACTTTGATTTCTTCAAAGTGCCCGAAAAGGTTGAGTGGGTTCTCACAGACCCTTCTCAGGAGGCTTGGACGCCGTTTATCGACAACGAGAAAGTACCTGAATCTACGAAACGCGAATGGGTGGGAGCTTTTCAACTCCTTGGCCGATCTAAAGTTTGA

Protein sequence

MSASGTSFQDPTRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCATRDLHRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTDASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETGTLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWTPFIDNEKVPESTKREWVGAFQLLGRSKV
Homology
BLAST of Lag0023244 vs. NCBI nr
Match: XP_022948610.1 (methyl-CpG-binding domain-containing protein 5-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 380.6 bits (976), Expect = 1.1e-101
Identity = 207/268 (77.24%), Postives = 215/268 (80.22%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HR +PDTSAT RSP  AD  N   LPPD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRNKPDTSATLRSPLPADPLNPKKLPPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  ERPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSERPNWLPPGWVVEDRVRASGATAGTVDKYYFDPKSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGN  SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGNVKSADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSK+
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKI 262

BLAST of Lag0023244 vs. NCBI nr
Match: XP_022998335.1 (methyl-CpG-binding domain-containing protein 5-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 379.8 bits (974), Expect = 1.8e-101
Identity = 208/268 (77.61%), Postives = 216/268 (80.60%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HRK+PDTSAT RSP  AD  N   L PD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRKKPDTSATLRSPLPADPLNPKKLAPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  +RPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSDRPNWLPPGWVVEDRVRASGATAGTVDKYYFDPNSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGNA SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGNAMSADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSKV
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKV 262

BLAST of Lag0023244 vs. NCBI nr
Match: XP_023523162.1 (methyl-CpG-binding domain-containing protein 5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 376.7 bits (966), Expect = 1.5e-100
Identity = 208/268 (77.61%), Postives = 215/268 (80.22%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HRK+PDTSAT RSP  AD  N   LPPD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGTHRKKPDTSATLRSPLPADPLNPKKLPPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  ERPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSERPNWLPPGWVVEDRVRASGATAGTVDKYYFDPNSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGN  SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQE WT
Sbjct: 181 TLRKRKKSLDGN--SADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEVWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSKV
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKV 260

BLAST of Lag0023244 vs. NCBI nr
Match: XP_022948611.1 (methyl-CpG-binding domain-containing protein 5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 376.3 bits (965), Expect = 2.0e-100
Identity = 207/268 (77.24%), Postives = 215/268 (80.22%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HR +PDTSAT RSP  AD  N   LPPD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRNKPDTSATLRSPLPADPLNPKKLPPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  ERPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSERPNWLPPGWVVEDRVRASGATAGTVDKYYFDPKSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGN  SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGN--SADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSK+
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKI 260

BLAST of Lag0023244 vs. NCBI nr
Match: KAG7036930.1 (Methyl-CpG-binding domain-containing protein 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 374.4 bits (960), Expect = 7.7e-100
Identity = 207/268 (77.24%), Postives = 214/268 (79.85%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HR +PDTSAT RSP   D  N   LPPD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRNKPDTSATLRSPLPPDPLNPKKLPPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  ERPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSERPNWLPPGWVVEDRVRASGATAGTVDKYYFDPNSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGN  SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGN--SADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSKV
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKV 260

BLAST of Lag0023244 vs. ExPASy Swiss-Prot
Match: Q9SNC0 (Methyl-CpG-binding domain-containing protein 5 OS=Arabidopsis thaliana OX=3702 GN=MBD5 PE=1 SV=1)

HSP 1 Score: 129.0 bits (323), Expect = 7.3e-29
Identity = 72/169 (42.60%), Postives = 99/169 (58.58%), Query Frame = 0

Query: 85  GNGDSPPSKKLSASRPTDASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPV 144
           G   + P  +  A+     S K A+    NWLPP W  E RVR SG  AGTVDK+Y++P+
Sbjct: 4   GTDQAQPPPENPATPVDSKSRKRATPGDDNWLPPDWRTEIRVRTSGTKAGTVDKFYYEPI 63

Query: 145 SGRRFRSKIEVLYFLETGTLKKRK-KSLDGNATSADGSE-------EPKTKKSSSNAKSA 204
           +GR+FRSK EVLY+LE GT KK+  K+ +   + ++ SE       + K+ K  +     
Sbjct: 64  TGRKFRSKNEVLYYLEHGTPKKKSVKTAENGDSHSEHSEGRGSARRQTKSNKKVTEPPPK 123

Query: 205 PLNFDFFKVPEKVEWVLTDPSQEAWTPFIDNEKVPESTKREWVGAFQLL 246
           PLNFDF  VPEKV W   + S+EAW PFI + K+ ES  ++W   F L+
Sbjct: 124 PLNFDFLNVPEKVTWTGINGSEEAWLPFIGDYKIQESVSQDWDRVFTLV 172

BLAST of Lag0023244 vs. ExPASy Swiss-Prot
Match: Q9LTJ1 (Methyl-CpG-binding domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 GN=MBD6 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 3.6e-28
Identity = 86/237 (36.29%), Postives = 121/237 (51.05%), Query Frame = 0

Query: 21  SASASHHLPPDPLLSAGSFIDSSAAPCATRDLHRKRPDTSATRRSPFRADHPNGNNLPPD 80
           S S +   PPDPLL++G+FI S+              D+SA RR              P 
Sbjct: 2   SDSVAGDFPPDPLLASGAFISSAGDGTL---------DSSAKRR--------------PI 61

Query: 81  PPAPGNGDSPPSKKLSASRPTD-----ASPKSASLERP--NWLPPGWVLEDRVRASGATA 140
               G   S  S ++  +  TD        KS     P  NWLPPGW +ED++R SGATA
Sbjct: 62  QGGIGISGSGESVRIGMANGTDQVNHQTESKSRKRAAPGDNWLPPGWRVEDKIRTSGATA 121

Query: 141 GTVDKYYFDPVSGRRFRSKIEVLYFLETGTLKKRKKSLDGNATSADGSEEPKTKKSSSNA 200
           G+VDKYY++P +GR+FRS+ EVLY+LE GT K+  K  +    + D  E   + + +  A
Sbjct: 122 GSVDKYYYEPNTGRKFRSRTEVLYYLEHGTSKRGTKKAENTYFNPDHFEGQGSNRVTRTA 181

Query: 201 -----KSAPLNFDFFKVPEKVEWVLTDPSQEAWTPFIDNEKVPESTKREWVGAFQLL 246
                   PL+FDF   P+KV W + +  +E W P I + KV +S +R+W  AF  +
Sbjct: 182 TVPPPPPPPLDFDFKNPPDKVSWSMANAGEEGWIPNIGDVKVQDSVRRDWSTAFTFI 215

BLAST of Lag0023244 vs. ExPASy Swiss-Prot
Match: Q9UBB5 (Methyl-CpG-binding domain protein 2 OS=Homo sapiens OX=9606 GN=MBD2 PE=1 SV=1)

HSP 1 Score: 50.4 bits (119), Expect = 3.3e-05
Identity = 23/44 (52.27%), Postives = 31/44 (70.45%), Query Frame = 0

Query: 116 LPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFL 160
           LPPGW  E+ +R SG +AG  D YYF P SG++FRSK ++  +L
Sbjct: 155 LPPGWKKEEVIRKSGLSAGKSDVYYFSP-SGKKFRSKPQLARYL 197

BLAST of Lag0023244 vs. ExPASy TrEMBL
Match: A0A6J1GAC7 (methyl-CpG-binding domain-containing protein 5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452236 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 5.2e-102
Identity = 207/268 (77.24%), Postives = 215/268 (80.22%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HR +PDTSAT RSP  AD  N   LPPD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRNKPDTSATLRSPLPADPLNPKKLPPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  ERPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSERPNWLPPGWVVEDRVRASGATAGTVDKYYFDPKSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGN  SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGNVKSADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSK+
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKI 262

BLAST of Lag0023244 vs. ExPASy TrEMBL
Match: A0A6J1KC76 (methyl-CpG-binding domain-containing protein 5-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111493004 PE=4 SV=1)

HSP 1 Score: 379.8 bits (974), Expect = 8.8e-102
Identity = 208/268 (77.61%), Postives = 216/268 (80.60%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HRK+PDTSAT RSP  AD  N   L PD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRKKPDTSATLRSPLPADPLNPKKLAPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  +RPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSDRPNWLPPGWVVEDRVRASGATAGTVDKYYFDPNSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGNA SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGNAMSADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSKV
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKV 262

BLAST of Lag0023244 vs. ExPASy TrEMBL
Match: A0A6J1G9P7 (methyl-CpG-binding domain-containing protein 5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452236 PE=4 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 9.8e-101
Identity = 207/268 (77.24%), Postives = 215/268 (80.22%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HR +PDTSAT RSP  AD  N   LPPD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRNKPDTSATLRSPLPADPLNPKKLPPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  ERPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSERPNWLPPGWVVEDRVRASGATAGTVDKYYFDPKSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGN  SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGN--SADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSK+
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKI 260

BLAST of Lag0023244 vs. ExPASy TrEMBL
Match: A0A6J1K9Z4 (methyl-CpG-binding domain-containing protein 5-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111493004 PE=4 SV=1)

HSP 1 Score: 374.0 bits (959), Expect = 4.8e-100
Identity = 207/268 (77.24%), Postives = 215/268 (80.22%), Query Frame = 0

Query: 1   MSASGTSFQDP-------TRPDFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT---- 60
           MSASGTSF DP       TRPD PS       HH PPDPLLS+GSFIDSS A C T    
Sbjct: 1   MSASGTSFPDPDWPRQGSTRPDLPS------PHHFPPDPLLSSGSFIDSSTARCGTLPSS 60

Query: 61  ----RDL---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTD 120
               RDL   HRK+PDTSAT RSP  AD  N   L PD P PGNGDS   KK+SASR TD
Sbjct: 61  PDYARDLDGNHRKKPDTSATLRSPLPADPLNPKKLAPDSPTPGNGDSSAPKKVSASRGTD 120

Query: 121 ASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETG 180
           ASPKSA  +RPNWLPPGWV+EDRVRASGATAGTVDKYYFDP SGRRFRSKIEVLYFLETG
Sbjct: 121 ASPKSALSDRPNWLPPGWVVEDRVRASGATAGTVDKYYFDPNSGRRFRSKIEVLYFLETG 180

Query: 181 TLKKRKKSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWT 240
           TL+KRKKSLDGN  SADGSEEPK+KKSSSNAKSAPLNFDFF VPEKVEWVLTDPSQEAWT
Sbjct: 181 TLRKRKKSLDGN--SADGSEEPKSKKSSSNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWT 240

Query: 241 PFIDNEKVPESTKREWVGAFQLLGRSKV 251
           PFI+NEKVPESTKREWV AFQLLGRSKV
Sbjct: 241 PFINNEKVPESTKREWVAAFQLLGRSKV 260

BLAST of Lag0023244 vs. ExPASy TrEMBL
Match: A0A6J1HLU8 (methyl-CpG-binding domain-containing protein 5-like OS=Cucurbita moschata OX=3662 GN=LOC111464796 PE=4 SV=1)

HSP 1 Score: 352.1 bits (902), Expect = 2.0e-93
Identity = 193/262 (73.66%), Postives = 208/262 (79.39%), Query Frame = 0

Query: 1   MSASGTSFQDPTRP-DFPSSASASASHHLPPDPLLSAGSFIDSSAAPCAT--------RD 60
           MSA GTS  DP  P   P+  +   S+HLPPDPLL AGSFIDSSAA C T        RD
Sbjct: 1   MSAFGTSLSDPDWPRRDPTRFNLPFSNHLPPDPLLDAGSFIDSSAARCGTLPSSPNHSRD 60

Query: 61  L---HRKRPDTSATRRSPFRADHPNGNNLPPDPPAPGNGDSPPSKKLSASRPTDASPKSA 120
           L   HRK+ +TS+T RSPFRA          DP   GNGDSP +KKLS SR TDASPKSA
Sbjct: 61  LDLNHRKKAETSSTLRSPFRA----------DPLNAGNGDSPATKKLSGSRGTDASPKSA 120

Query: 121 SLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETGTLKKRK 180
           S E+PNWLPPGW++EDRVR SGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETGTL+KRK
Sbjct: 121 SSEKPNWLPPGWIMEDRVRTSGATAGTVDKYYFDPVSGRRFRSKIEVLYFLETGTLRKRK 180

Query: 181 KSLDGNATSADGSEEPKTKKSSSNAKSAPLNFDFFKVPEKVEWVLTDPSQEAWTPFIDNE 240
           KSLDGN  SADG EEPK KKSS+NAKSAPLNFDFF VPEKVEWVLTDPSQEAWTPFI+NE
Sbjct: 181 KSLDGNGMSADGPEEPKNKKSSNNAKSAPLNFDFFNVPEKVEWVLTDPSQEAWTPFINNE 240

Query: 241 KVPESTKREWVGAFQLLGRSKV 251
           KVP+STKREWV AF+LL RSKV
Sbjct: 241 KVPQSTKREWVAAFKLLSRSKV 252

BLAST of Lag0023244 vs. TAIR 10
Match: AT3G46580.1 (methyl-CPG-binding domain protein 5 )

HSP 1 Score: 129.0 bits (323), Expect = 5.2e-30
Identity = 72/169 (42.60%), Postives = 99/169 (58.58%), Query Frame = 0

Query: 85  GNGDSPPSKKLSASRPTDASPKSASLERPNWLPPGWVLEDRVRASGATAGTVDKYYFDPV 144
           G   + P  +  A+     S K A+    NWLPP W  E RVR SG  AGTVDK+Y++P+
Sbjct: 4   GTDQAQPPPENPATPVDSKSRKRATPGDDNWLPPDWRTEIRVRTSGTKAGTVDKFYYEPI 63

Query: 145 SGRRFRSKIEVLYFLETGTLKKRK-KSLDGNATSADGSE-------EPKTKKSSSNAKSA 204
           +GR+FRSK EVLY+LE GT KK+  K+ +   + ++ SE       + K+ K  +     
Sbjct: 64  TGRKFRSKNEVLYYLEHGTPKKKSVKTAENGDSHSEHSEGRGSARRQTKSNKKVTEPPPK 123

Query: 205 PLNFDFFKVPEKVEWVLTDPSQEAWTPFIDNEKVPESTKREWVGAFQLL 246
           PLNFDF  VPEKV W   + S+EAW PFI + K+ ES  ++W   F L+
Sbjct: 124 PLNFDFLNVPEKVTWTGINGSEEAWLPFIGDYKIQESVSQDWDRVFTLV 172

BLAST of Lag0023244 vs. TAIR 10
Match: AT5G59380.1 (methyl-CPG-binding domain 6 )

HSP 1 Score: 126.7 bits (317), Expect = 2.6e-29
Identity = 86/237 (36.29%), Postives = 121/237 (51.05%), Query Frame = 0

Query: 21  SASASHHLPPDPLLSAGSFIDSSAAPCATRDLHRKRPDTSATRRSPFRADHPNGNNLPPD 80
           S S +   PPDPLL++G+FI S+              D+SA RR              P 
Sbjct: 2   SDSVAGDFPPDPLLASGAFISSAGDGTL---------DSSAKRR--------------PI 61

Query: 81  PPAPGNGDSPPSKKLSASRPTD-----ASPKSASLERP--NWLPPGWVLEDRVRASGATA 140
               G   S  S ++  +  TD        KS     P  NWLPPGW +ED++R SGATA
Sbjct: 62  QGGIGISGSGESVRIGMANGTDQVNHQTESKSRKRAAPGDNWLPPGWRVEDKIRTSGATA 121

Query: 141 GTVDKYYFDPVSGRRFRSKIEVLYFLETGTLKKRKKSLDGNATSADGSEEPKTKKSSSNA 200
           G+VDKYY++P +GR+FRS+ EVLY+LE GT K+  K  +    + D  E   + + +  A
Sbjct: 122 GSVDKYYYEPNTGRKFRSRTEVLYYLEHGTSKRGTKKAENTYFNPDHFEGQGSNRVTRTA 181

Query: 201 -----KSAPLNFDFFKVPEKVEWVLTDPSQEAWTPFIDNEKVPESTKREWVGAFQLL 246
                   PL+FDF   P+KV W + +  +E W P I + KV +S +R+W  AF  +
Sbjct: 182 TVPPPPPPPLDFDFKNPPDKVSWSMANAGEEGWIPNIGDVKVQDSVRRDWSTAFTFI 215

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022948610.11.1e-10177.24methyl-CpG-binding domain-containing protein 5-like isoform X1 [Cucurbita moscha... [more]
XP_022998335.11.8e-10177.61methyl-CpG-binding domain-containing protein 5-like isoform X1 [Cucurbita maxima... [more]
XP_023523162.11.5e-10077.61methyl-CpG-binding domain-containing protein 5-like [Cucurbita pepo subsp. pepo][more]
XP_022948611.12.0e-10077.24methyl-CpG-binding domain-containing protein 5-like isoform X2 [Cucurbita moscha... [more]
KAG7036930.17.7e-10077.24Methyl-CpG-binding domain-containing protein 6, partial [Cucurbita argyrosperma ... [more]
Match NameE-valueIdentityDescription
Q9SNC07.3e-2942.60Methyl-CpG-binding domain-containing protein 5 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9LTJ13.6e-2836.29Methyl-CpG-binding domain-containing protein 6 OS=Arabidopsis thaliana OX=3702 G... [more]
Q9UBB53.3e-0552.27Methyl-CpG-binding domain protein 2 OS=Homo sapiens OX=9606 GN=MBD2 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1GAC75.2e-10277.24methyl-CpG-binding domain-containing protein 5-like isoform X1 OS=Cucurbita mosc... [more]
A0A6J1KC768.8e-10277.61methyl-CpG-binding domain-containing protein 5-like isoform X1 OS=Cucurbita maxi... [more]
A0A6J1G9P79.8e-10177.24methyl-CpG-binding domain-containing protein 5-like isoform X2 OS=Cucurbita mosc... [more]
A0A6J1K9Z44.8e-10077.24methyl-CpG-binding domain-containing protein 5-like isoform X2 OS=Cucurbita maxi... [more]
A0A6J1HLU82.0e-9373.66methyl-CpG-binding domain-containing protein 5-like OS=Cucurbita moschata OX=366... [more]
Match NameE-valueIdentityDescription
AT3G46580.15.2e-3042.60methyl-CPG-binding domain protein 5 [more]
AT5G59380.12.6e-2936.29methyl-CPG-binding domain 6 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001739Methyl-CpG DNA bindingPFAMPF01429MBDcoord: 111..208
e-value: 2.8E-13
score: 49.4
IPR001739Methyl-CpG DNA bindingPROSITEPS50982MBDcoord: 106..178
score: 14.729399
NoneNo IPR availableGENE3D3.30.890.10coord: 86..199
e-value: 1.0E-21
score: 79.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..25
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 168..195
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 91..108
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 48..65
NoneNo IPR availablePANTHERPTHR12396:SF46METHYL-CPG BINDING DOMAIN PROTEIN-LIKE, ISOFORM Ccoord: 102..236
NoneNo IPR availablePANTHERPTHR12396METHYL-CPG BINDING PROTEIN, MBDcoord: 102..236
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 107..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0023244.1Lag0023244.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding