Lag0020167 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0020167 vs. NCBI nr
Match: XP_022984356.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1396.3 bits (3613), Expect = 0.0e+00
Identity = 688/773 (89.00%), Postives = 731/773 (94.57%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+PAKACSRKL+GARYF+KGLEAAYGHP NN+ FQDYLSPRD SGHGTHVSSVA 
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLT+F NLVYPEVSDLDD RACESLSLNDTWA+
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWAS 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDDY+DNTH+TSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLD+GMQ
Sbjct: 421 GNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIG STTRVGQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK RNSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
           VIFAEGRPM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI+K
Sbjct: 601 VIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 720
           RSI+CPKRRPSILDINVPSITIPSL +SV+LTRTV NVG +NS+YKAAI  PPGI I VK
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVK 720

Query: 721 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           PRILKFNHK + ISFTVT+S+N RVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721 PRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Lag0020167 vs. NCBI nr
Match: KAG7014677.1 (Subtilisin-like protease SBT3.3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1394.0 bits (3607), Expect = 0.0e+00
Identity = 686/773 (88.75%), Postives = 732/773 (94.70%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+PAKACSRKL+GARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD+GMQ
Sbjct: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIG STTRVGQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
           VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+N+SAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNDSAISGITK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 720
           RSI+CPKRRPSILDINVPSITIPSL + V+LTRTV NVG +NS+YKAAI  PPGI I +K
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAIK 720

Query: 721 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           PRILKFNHKM+ ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Lag0020167 vs. NCBI nr
Match: XP_022922598.1 (subtilisin-like protease SBT3.9 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 687/773 (88.87%), Postives = 731/773 (94.57%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+PAKACSRKL+GARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVD SFLVAITLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361 SSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD+GMQ
Sbjct: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIG STTRVGQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
           VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 720
           RSI+CPKRRPSILDINVPSITIPSL + V+LTRTV NVG +NS+YKAAI  PPGI I VK
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           PRILKFNHKM+ ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Lag0020167 vs. NCBI nr
Match: XP_022141072.1 (subtilisin-like protease SBT3.9 [Momordica charantia])

HSP 1 Score: 1391.3 bits (3600), Expect = 0.0e+00
Identity = 684/775 (88.26%), Postives = 731/775 (94.32%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           MSSFAHGKSS TTI L+FISFFT L+DLCTL++  DAKS+VY+VYMG RPHDNEELLVKT
Sbjct: 1   MSSFAHGKSSSTTILLLFISFFTLLSDLCTLLVPVDAKSHVYVVYMGKRPHDNEELLVKT 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQEA ADS+VYNYKYGFSGFAAKLTMAQAQ +SELP+VIKVIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQKISELPTVIKVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPR  NSLLQKSRMGNGAIIGLLDTGIWPESEVF D+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTGIWPESEVFSDNGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+P KACSRKL+GARYFIKGLEAAYGHP+NN+GFQDYLSPRDY+GHGTHVSSVA 
Sbjct: 181 ESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           GSFV NV YHGLAVGTVRGGAPQSRLAMYKVCW+LNGGVCSD DILKAIDQAI+DGVDVL
Sbjct: 241 GSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGPTFPSYSDVDMH+G+AIG FHA+ KGI VVGAAGN GPAAYSVANIEPWLLTVAA
Sbjct: 301 SLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTT+GQGMFSGKL RFHNLVYPEVSDL+D RACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPEVSDLEDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDD++DNTH TS SVKKVG LGLIVAKNPTK VEPFI++FPCVQ+SL++GM 
Sbjct: 421 GNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMH 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQV IGPS TR+GQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK++NSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE
Sbjct: 541 VPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
            IFAEGRP+KSAGPFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCAMG+N+SAISGITK
Sbjct: 601 TIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPP--PGIAIF 720
           + I+CPKRRPSILD NVPSITIP L++SV +TRTV NVG VNSTYKAAI  P  PGIAI 
Sbjct: 661 KPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIA 720

Query: 721 VKPRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           VKPR+LKFNHK + ISFTVTVS+NHRVTTGYCFGSLTWLDG+HSVRIPISVRT+I
Sbjct: 721 VKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 775

BLAST of Lag0020167 vs. NCBI nr
Match: XP_023551712.1 (subtilisin-like protease SBT3.9 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1390.2 bits (3597), Expect = 0.0e+00
Identity = 685/773 (88.62%), Postives = 730/773 (94.44%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+PAKACSRKL+GARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSKFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDD++D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD GMQ
Sbjct: 421 GNVVLCFASDDFNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDTGMQ 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIG STTRVGQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
           VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 720
           RSI+CPKRRPSILDINVPSITIPSL + V+LTRTV NVG +NS+YKAAI  PPGI I VK
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           PRILKFNHKM+ I FTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721 PRILKFNHKMKSIPFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Lag0020167 vs. ExPASy Swiss-Prot
Match: Q9MAP5 (Subtilisin-like protease SBT3.3 OS=Arabidopsis thaliana OX=3702 GN=SBT3.3 PE=2 SV=1)

HSP 1 Score: 823.5 bits (2126), Expect = 1.9e-237
Identity = 418/773 (54.08%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 8   KSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 67
           +S R++I LV +S  T LN        ++ +S V+IVY+G + H + E + ++HH +LAS
Sbjct: 2   RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 68  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 127
           +LGS++ + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  H++ TTR+W+Y
Sbjct: 62  LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 128 LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFN 187
           L LS   P +LL  + MG+  IIG++DTG+WPESE F D+G+GP+P +WKG CE GE F 
Sbjct: 122 LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 188 PAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 247
            +  C+RKL+GA+YFI G   A    FN T  +DY+S RD+ GHGTHV+S+AGGSFVPNV
Sbjct: 182 -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 248 GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 307
            Y GLA GT+RGGAP++R+AMYK CW    L G  CSD DI+KAID+AI+DGVDVLS+SL
Sbjct: 242 SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 308 GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 367
               P  S+ D+ D  A G FHAVAKGI VV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302 VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPR---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ  ++G      +LVYPE +  ++      CESL+LN  +  A
Sbjct: 362 RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 428 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 487
             VVLCF +   +      +  VK  GGLGLI+++NP   + P  D+FPCV +  ++G  
Sbjct: 422 MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL+YIRSTR+P VKI  S T  GQ + T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482 ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 548 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 607
             P+D      +A +SGTSMATP +S ++ALLK+LH  WSPAA +SAIVTTAW +DP+GE
Sbjct: 542 TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 667
            IFAEG   K + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ G+N+S+IS +  
Sbjct: 602 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 668 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 727
           +   C   +PS+LD+N+PSITIP+L+  V LTRTV NVGLV+S YK ++ PP G+ + V 
Sbjct: 662 QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 728 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           P  L FN K   +SFTV VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Lag0020167 vs. ExPASy Swiss-Prot
Match: Q9ZSB0 (Subtilisin-like protease SBT3.9 OS=Arabidopsis thaliana OX=3702 GN=SBT3.9 PE=3 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 4.0e-235
Identity = 428/766 (55.87%), Postives = 549/766 (71.67%), Query Frame = 0

Query: 10  SRTTIFL-VFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASV 69
           S+T +FL +F+S    LN   + V+   A+S VY+VY+G + HDN E + ++HH +L S+
Sbjct: 2   SKTILFLALFLSI--VLNVQISFVV---AESKVYVVYLGEKEHDNPESVTESHHQMLWSL 61

Query: 70  LGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYL 129
           LGS+EA  DS+VY+Y++GFSGFAAKLT +QAQ +SELP V++VIPN L++M TTR+WDYL
Sbjct: 62  LGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYL 121

Query: 130 QLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFNP 189
            +SP   +SLLQK+ MG   I+G++D+G+WPESE+F D G GP+PSRWKG CE GELFN 
Sbjct: 122 GVSPGNSDSLLQKANMGYNVIVGVIDSGVWPESEMFNDKGFGPIPSRWKGGCESGELFNA 181

Query: 190 AKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVG 249
           +  C+RKL+GA+YF+ GL A +G   N T   +YLSPRD++GHGTHV+S  GGSF+PNV 
Sbjct: 182 SIHCNRKLIGAKYFVDGLVAEFG-VVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVS 241

Query: 250 YHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVLSLSLGPTF 309
           Y GL  GT RGGAP   +A+YK CW    G CS  D+LKA+D+AI+DGVD+LSLSLGP+ 
Sbjct: 242 YVGLGRGTARGGAPGVHIAVYKACW---SGYCSGADVLKAMDEAIHDGVDILSLSLGPSV 301

Query: 310 PSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSFL 369
           P + +    +  ++GAFHAVAKGI VV AAGN+GP A +++N+ PW+LTVAA++ DRSF 
Sbjct: 302 PLFPET---EHTSVGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFP 361

Query: 370 VAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLN-DTWAAGNVVLCF 429
            AITLGNN T +GQ ++ G    F  L YPE     D   CE LS N ++   G VVLCF
Sbjct: 362 TAITLGNNITILGQAIYGGPELGFVGLTYPESPLSGD---CEKLSANPNSTMEGKVVLCF 421

Query: 430 ASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQILNYIRS 489
           A+      ++    +V   GGLGLI+AKNPT ++ P    FP V I  ++G  IL YIRS
Sbjct: 422 AA---STPSNAAIAAVINAGGLGLIMAKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRS 481

Query: 490 TRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK 549
           TR+P VKI  S T  GQS+ST VA FSSRGPNSV+PAILKPDIAAPGV ILAA+ P+   
Sbjct: 482 TRSPIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSI 541

Query: 550 ERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGEVIFAEGR 609
               +A +SGTSMATP VS +V LLKSLH  WSP+AIKSAIVTTAW +DP GE IFA+G 
Sbjct: 542 NDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGS 601

Query: 610 PMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSITCPK 669
             K A PFD+GGG++NP KA+ PGL+YDM   DY+ Y C++ +++ +IS +  +   CP 
Sbjct: 602 SRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGKITVCPN 661

Query: 670 RRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVKPRILKFN 729
            +PS+LD+N+PSITIP+LR  V LTRTV NVG VNS YK  I PP GI + V P  L F+
Sbjct: 662 PKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFD 721

Query: 730 HKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           +   + SFTV VST H+V TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 YTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVRTQI 748

BLAST of Lag0020167 vs. ExPASy Swiss-Prot
Match: Q8L7I2 (Subtilisin-like protease SBT3.6 OS=Arabidopsis thaliana OX=3702 GN=SBT3.6 PE=2 SV=1)

HSP 1 Score: 815.1 bits (2104), Expect = 6.9e-235
Identity = 410/770 (53.25%), Postives = 545/770 (70.78%), Query Frame = 0

Query: 11  RTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASVLG 70
           RT+I++V +S    LN   + V  + AK  V+IVY+G + HD+ E + ++HH +L S+LG
Sbjct: 5   RTSIYVV-LSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLG 64

Query: 71  SQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYLQL 130
           S+E + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  +K+ TTR+WDYL L
Sbjct: 65  SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 124

Query: 131 SPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFNPAK 190
           S   P SLL ++ MG   IIG++DTG+WPESEVF D G GPVPS WKG CE GE FN + 
Sbjct: 125 SAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN 184

Query: 191 ACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVGYH 250
            C++KL+GA+YFI G   A    FN+T   D++SPRD  GHGTHVS++AGGSFVPN+ Y 
Sbjct: 185 -CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 244

Query: 251 GLAVGTVRGGAPQSRLAMYKVCWRLNGG---VCSDIDILKAIDQAIYDGVDVLSLSLGPT 310
           GLA GTVRGGAP++ +AMYK CW L+      CS  DILKA+D+A++DGVDVLS+SLG +
Sbjct: 245 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSS 304

Query: 311 FPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSF 370
            P Y + D+ DGI  GAFHAV KGI VV + GNSGP + +V N  PW++TVAA+++DRSF
Sbjct: 305 VPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSF 364

Query: 371 LVAITLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAAGNV 430
              +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N +    G V
Sbjct: 365 ATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKV 424

Query: 431 VLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQILN 490
           VLCF +  Y       +  VK+ GGLG+I+A++P  A++P +D+FPCV +  ++G  IL 
Sbjct: 425 VLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILL 484

Query: 491 YIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPP 550
           Y RS+ +P VKI PS T VGQ + T VA FSSRGPNS+APAILKPDIAAPGV+ILAA   
Sbjct: 485 YTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTN 544

Query: 551 SDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGEVIF 610
           +   ++  +  +SGTSMA P +S + ALLK+LH  WSPAAI+SAIVTTAW +DP+GE IF
Sbjct: 545 TTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIF 604

Query: 611 AEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSI 670
           AEG P K A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C++G+N ++IS +  ++ 
Sbjct: 605 AEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTT 664

Query: 671 TCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVKPRI 730
            C   +PS+LD N+PSITIP+L+  V +TRTV NVG +NS Y+  + PP G  + V P  
Sbjct: 665 VCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPET 724

Query: 731 LKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           L FN   +++ F V VST H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 725 LVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Lag0020167 vs. ExPASy Swiss-Prot
Match: Q9SZY3 (Subtilisin-like protease SBT3.8 OS=Arabidopsis thaliana OX=3702 GN=SBT3.8 PE=3 SV=1)

HSP 1 Score: 812.4 bits (2097), Expect = 4.5e-234
Identity = 417/773 (53.95%), Postives = 541/773 (69.99%), Query Frame = 0

Query: 8   KSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 67
           KS RT IF+  I     LN L T V  A A+S V+IVY+G + HD+ E + ++HH +L S
Sbjct: 2   KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 68  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 127
           +LGS+E +  S+V++Y++GFSGFAAKLT +QA+ +++LP V+ V P+  +++ TTR+WDY
Sbjct: 62  LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 128 LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFN 187
           L LS   P +LL  + MG   IIG++D+G+WPESEVF D+G+GPVPS WKG C  GE F 
Sbjct: 122 LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 188 PAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 247
            ++ C++KL+GA+YFI G  A +   FN+T   D++SPRD SGHGTHV+++AGGS+VP++
Sbjct: 182 SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 248 GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 307
            Y GLA GTVRGGAP++R+AMYK CW   R +   CS  DILKA+D+A++DGVDVLSLS+
Sbjct: 242 SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 308 GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 367
           G  FP + + D+   IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302 GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ M++G    F +LVYPE    S+      CE L  N     A
Sbjct: 362 RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 428 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 487
           G VVLCF +             VK+ GGLG+IVA+NP   + P  D+FPCV +  ++G  
Sbjct: 422 GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL YIRST  P VKI PS T VGQ + T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482 ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 548 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 607
              +       + F+SGTSMA P +S +VALLK+LH  WSPAAI+SAIVTTAW +DP+GE
Sbjct: 542 TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 667
            IFAEG P K A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C++G+N ++IS +  
Sbjct: 602 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 668 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 727
           +   C   +PS+LD N+PSITIP+L+  V LTRT+ NVG + S YK  I PP GI + V 
Sbjct: 662 KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 728 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           P  L FN   +R+SF V VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767

BLAST of Lag0020167 vs. ExPASy Swiss-Prot
Match: Q9SZY2 (Subtilisin-like protease SBT3.7 OS=Arabidopsis thaliana OX=3702 GN=SBT3.7 PE=3 SV=2)

HSP 1 Score: 810.4 bits (2092), Expect = 1.7e-233
Identity = 410/772 (53.11%), Postives = 550/772 (71.24%), Query Frame = 0

Query: 8   KSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 67
           ++ RT+IF+V +S    LN     +  A A+S V+IVY+G + HD+ E + ++HH +L S
Sbjct: 2   RNHRTSIFVV-LSLVIILNGQSGFLPRAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 68  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 127
           +LGS+E +  S+V+++++GFSGFAAKLT +QA+ +++LP V+ VIP+R +K  TTR+WDY
Sbjct: 62  LLGSKEEAHGSMVHSFRHGFSGFAAKLTESQAKKIADLPEVVHVIPDRFYKPATTRTWDY 121

Query: 128 LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFN 187
           L LSP  P +LL ++ MG   IIG++D+G+WPESEVF D+ +GPVPS WKG CE GE FN
Sbjct: 122 LGLSPTNPKNLLNQTNMGEQMIIGIIDSGVWPESEVFNDNEIGPVPSHWKGGCESGEDFN 181

Query: 188 PAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 247
            +  C++KL+GA+YFI    A +   FN++   D++SPR Y+GHGTHV+++AGGS+VPN 
Sbjct: 182 -SSHCNKKLIGAKYFINAFLATH-ESFNSSESLDFISPRGYNGHGTHVATIAGGSYVPNT 241

Query: 248 GYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGV--CSDIDILKAIDQAIYDGVDVLSLSLG 307
            Y GLA GTVRGGAP++R+A+YK CW L+  +  CS  DILKA+D+AI+DGVDVLSLSLG
Sbjct: 242 SYKGLAGGTVRGGAPRARIAVYKTCWYLDLDIAACSSADILKAMDEAIHDGVDVLSLSLG 301

Query: 308 PTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDR 367
              P Y + D+ DGIA GAFHAV KGI VV AAGN+GPAA +V N  PW+LTVAA+++DR
Sbjct: 302 -FEPLYPETDVRDGIATGAFHAVLKGITVVCAAGNAGPAAQTVGNTAPWILTVAATTLDR 361

Query: 368 SFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAAG 427
           SF+  +TLGNN   +GQ +++G    F +LVYPE    S+      CE L +N +   AG
Sbjct: 362 SFVTPMTLGNNKVILGQAIYTGTEVGFTSLVYPENPGNSNESFSGTCERLLINSNRTMAG 421

Query: 428 NVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQI 487
            VVLCF    Y  +    +  VK+ GGLG+I+A  P   + P +D+FPCV +  ++G  I
Sbjct: 422 KVVLCFTESPYSISVTRAAHYVKRAGGLGVIIAGQPGNVLRPCLDDFPCVAVDYELGTYI 481

Query: 488 LNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAV 547
           L YIRS  +P VKI PS T +GQ + T VA FSSRGPN ++ AILKPDIAAPGV+ILAA 
Sbjct: 482 LFYIRSNGSPVVKIQPSRTLIGQPVGTKVASFSSRGPNPISAAILKPDIAAPGVSILAAT 541

Query: 548 PPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGEV 607
             +       + F+SGTSMATP +S IVALLK+LH  WSPAAI+SAIVTTAW +DP+GE 
Sbjct: 542 TTNTTFNDRGFIFLSGTSMATPTISGIVALLKALHPDWSPAAIRSAIVTTAWRTDPFGEQ 601

Query: 608 IFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKR 667
           IFAEG P K A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C++G+N ++IS +  +
Sbjct: 602 IFAEGSPRKPADPFDYGGGLVNPEKATKPGLVYDLGLEDYVLYMCSVGYNETSISQLVGK 661

Query: 668 SITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVKP 727
              C   +PS+LD N+PSITIP+L+  V L RT+ NVG + S Y+ A+ PP G  + V P
Sbjct: 662 GTVCSYPKPSVLDFNLPSITIPNLKEEVTLPRTLTNVGPLESVYRVAVEPPLGTQVTVTP 721

Query: 728 RILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
             L FN   +R+SF V+VST H++ TGY FGSLTW D +H+V IP+SVRT +
Sbjct: 722 ETLVFNSTTKRVSFKVSVSTTHKINTGYYFGSLTWSDSLHNVTIPLSVRTQL 769

BLAST of Lag0020167 vs. ExPASy TrEMBL
Match: A0A6J1J508 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1396.3 bits (3613), Expect = 0.0e+00
Identity = 688/773 (89.00%), Postives = 731/773 (94.57%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHK+Q
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKIQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+PAKACSRKL+GARYF+KGLEAAYGHP NN+ FQDYLSPRD SGHGTHVSSVA 
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTTMGQGMFSGKLT+F NLVYPEVSDLDD RACESLSLNDTWA+
Sbjct: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWAS 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDDY+DNTH+TSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLD+GMQ
Sbjct: 421 GNVVLCFASDDYNDNTHNTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIG STTRVGQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK RNSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKNRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
           VIFAEGRPM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI+K
Sbjct: 601 VIFAEGRPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 720
           RSI+CPKRRPSILDINVPSITIPSL +SV+LTRTV NVG +NS+YKAAI  PPGI I VK
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVK 720

Query: 721 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           PRILKFNHK + ISFTVT+S+N RVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721 PRILKFNHKTKSISFTVTISSNQRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Lag0020167 vs. ExPASy TrEMBL
Match: A0A6J1E757 (subtilisin-like protease SBT3.9 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1393.3 bits (3605), Expect = 0.0e+00
Identity = 687/773 (88.87%), Postives = 731/773 (94.57%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           M SFAHGKSS TTIFLV IS FTAL+ LCTL I   AKSNVYIVYMG +PHDNEELLVK 
Sbjct: 1   MGSFAHGKSSSTTIFLVCISVFTALDHLCTLHIRVAAKSNVYIVYMGQKPHDNEELLVKA 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQE S DSLVY+YKYGFSGFAAKLTMAQAQM+SELPSV++VIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEGSVDSLVYSYKYGFSGFAAKLTMAQAQMISELPSVVEVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+PAKACSRKL+GARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSVA 
Sbjct: 181 ESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVL
Sbjct: 241 GFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAA
Sbjct: 301 SLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVD SFLVAITLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTWAA
Sbjct: 361 SSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD+GMQ
Sbjct: 421 GNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIGMQ 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQVKIG STTRVGQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG 
Sbjct: 541 VPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGA 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
           VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGITK
Sbjct: 601 VIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGITK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 720
           RSI+CPKRRPSILDINVPSITIPSL + V+LTRTV NVG +NS+YKAAI  PPGI I VK
Sbjct: 661 RSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIAVK 720

Query: 721 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           PRILKFNHKM+ ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 721 PRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 773

BLAST of Lag0020167 vs. ExPASy TrEMBL
Match: A0A6J1CIU2 (subtilisin-like protease SBT3.9 OS=Momordica charantia OX=3673 GN=LOC111011560 PE=3 SV=1)

HSP 1 Score: 1391.3 bits (3600), Expect = 0.0e+00
Identity = 684/775 (88.26%), Postives = 731/775 (94.32%), Query Frame = 0

Query: 1   MSSFAHGKSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKT 60
           MSSFAHGKSS TTI L+FISFFT L+DLCTL++  DAKS+VY+VYMG RPHDNEELLVKT
Sbjct: 1   MSSFAHGKSSSTTILLLFISFFTLLSDLCTLLVPVDAKSHVYVVYMGKRPHDNEELLVKT 60

Query: 61  HHGVLASVLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQ 120
           HHGVLASVLGSQEA ADS+VYNYKYGFSGFAAKLTMAQAQ +SELP+VIKVIPNRLHKMQ
Sbjct: 61  HHGVLASVLGSQEAPADSMVYNYKYGFSGFAAKLTMAQAQKISELPTVIKVIPNRLHKMQ 120

Query: 121 TTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGIC 180
           TTRSWDYLQLSPR  NSLLQKSRMGNGAIIGLLDTGIWPESEVF D+GLGPVPSRWKGIC
Sbjct: 121 TTRSWDYLQLSPRLQNSLLQKSRMGNGAIIGLLDTGIWPESEVFSDNGLGPVPSRWKGIC 180

Query: 181 EPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAG 240
           E GELF+P KACSRKL+GARYFIKGLEAAYGHP+NN+GFQDYLSPRDY+GHGTHVSSVA 
Sbjct: 181 ESGELFSPGKACSRKLIGARYFIKGLEAAYGHPYNNSGFQDYLSPRDYAGHGTHVSSVAS 240

Query: 241 GSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVL 300
           GSFV NV YHGLAVGTVRGGAPQSRLAMYKVCW+LNGGVCSD DILKAIDQAI+DGVDVL
Sbjct: 241 GSFVANVSYHGLAVGTVRGGAPQSRLAMYKVCWQLNGGVCSDADILKAIDQAIHDGVDVL 300

Query: 301 SLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAA 360
           SLSLGPTFPSYSDVDMH+G+AIG FHA+ KGI VVGAAGN GPAAYSVANIEPWLLTVAA
Sbjct: 301 SLSLGPTFPSYSDVDMHNGVAIGTFHAIVKGIVVVGAAGNYGPAAYSVANIEPWLLTVAA 360

Query: 361 SSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAA 420
           SSVDRSFLVAITLGNNWTT+GQGMFSGKL RFHNLVYPEVSDL+D RACESLSLNDTWAA
Sbjct: 361 SSVDRSFLVAITLGNNWTTVGQGMFSGKLRRFHNLVYPEVSDLEDERACESLSLNDTWAA 420

Query: 421 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 480
           GNVVLCFASDD++DNTH TS SVKKVG LGLIVAKNPTK VEPFI++FPCVQ+SL++GM 
Sbjct: 421 GNVVLCFASDDFNDNTHHTSRSVKKVGALGLIVAKNPTKDVEPFINHFPCVQVSLEIGMH 480

Query: 481 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540
           ILNYIRSTRNPQV IGPS TR+GQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA
Sbjct: 481 ILNYIRSTRNPQVNIGPSRTRIGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 540

Query: 541 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600
           VPPSDPK++NSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE
Sbjct: 541 VPPSDPKDKNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 600

Query: 601 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 660
            IFAEGRP+KSAGPFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCAMG+N+SAISGITK
Sbjct: 601 TIFAEGRPIKSAGPFDFGGGIVNPNKAVDPGLVYDMGMADYIRYFCAMGYNDSAISGITK 660

Query: 661 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPP--PGIAIF 720
           + I+CPKRRPSILD NVPSITIP L++SV +TRTV NVG VNSTYKAAI  P  PGIAI 
Sbjct: 661 KPISCPKRRPSILDTNVPSITIPGLKHSVTVTRTVTNVGAVNSTYKAAIETPPGPGIAIA 720

Query: 721 VKPRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           VKPR+LKFNHK + ISFTVTVS+NHRVTTGYCFGSLTWLDG+HSVRIPISVRT+I
Sbjct: 721 VKPRMLKFNHKRKIISFTVTVSSNHRVTTGYCFGSLTWLDGVHSVRIPISVRTDI 775

BLAST of Lag0020167 vs. ExPASy TrEMBL
Match: A0A6J1E3V3 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430558 PE=3 SV=1)

HSP 1 Score: 1204.1 bits (3114), Expect = 0.0e+00
Identity = 588/655 (89.77%), Postives = 626/655 (95.57%), Query Frame = 0

Query: 119 MQTTRSWDYLQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKG 178
           MQTTRSWDYLQLSPRFPNSLL+KSRMG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKG
Sbjct: 1   MQTTRSWDYLQLSPRFPNSLLRKSRMGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKG 60

Query: 179 ICEPGELFNPAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSV 238
           ICE GELF+PAKACSRKL+GARYF+KGLEAAYGHP NN+ FQDY SPRD SGHGTHVSSV
Sbjct: 61  ICESGELFSPAKACSRKLIGARYFVKGLEAAYGHPLNNSEFQDYWSPRDRSGHGTHVSSV 120

Query: 239 AGGSFVPNVGYHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVD 298
           A G FVPNV YHGLAVGTVRGGAP SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVD
Sbjct: 121 ASGFFVPNVSYHGLAVGTVRGGAPHSRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVD 180

Query: 299 VLSLSLGPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTV 358
           VLSLSLGP+FPSYSDVDM +GIAIGAFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTV
Sbjct: 181 VLSLSLGPSFPSYSDVDMRNGIAIGAFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTV 240

Query: 359 AASSVDRSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTW 418
           AASSVD SFLVAITLGNNWTTMGQGMFSGKLT+FHNLVYPEVSDLDD RACESLSLNDTW
Sbjct: 241 AASSVDCSFLVAITLGNNWTTMGQGMFSGKLTKFHNLVYPEVSDLDDERACESLSLNDTW 300

Query: 419 AAGNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVG 478
           AAGNVVLCFASDDY+D+TH+TS SVKKVGGLGLIVAKNPTKAVEPFI+NFPCVQISLD+G
Sbjct: 301 AAGNVVLCFASDDYNDDTHNTSSSVKKVGGLGLIVAKNPTKAVEPFINNFPCVQISLDIG 360

Query: 479 MQILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 538
           MQILNYIRSTRNPQVKIG STTRVGQ LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL
Sbjct: 361 MQILNYIRSTRNPQVKIGASTTRVGQPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAIL 420

Query: 539 AAVPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPY 598
           AAVPPSDPK+RNSYAFISGTSMATPHVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPY
Sbjct: 421 AAVPPSDPKDRNSYAFISGTSMATPHVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPY 480

Query: 599 GEVIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGI 658
           G VIFAEG+PM SA PFDFGGGIVNPNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI
Sbjct: 481 GAVIFAEGQPMMSARPFDFGGGIVNPNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGI 540

Query: 659 TKRSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIF 718
           TKRSI+CPKRRPSILDINVPSITIPSL + V+LTRTV NVG +NS+YKAAI  PPGI I 
Sbjct: 541 TKRSISCPKRRPSILDINVPSITIPSLTHPVSLTRTVTNVGAINSSYKAAIEAPPGITIA 600

Query: 719 VKPRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           VKPRILKFNHKM+ ISFTVT+S+NHRVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 601 VKPRILKFNHKMKSISFTVTISSNHRVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 655

BLAST of Lag0020167 vs. ExPASy TrEMBL
Match: A0A6J1J1Z1 (subtilisin-like protease SBT3.3 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482683 PE=3 SV=1)

HSP 1 Score: 1159.1 bits (2997), Expect = 0.0e+00
Identity = 566/630 (89.84%), Postives = 601/630 (95.40%), Query Frame = 0

Query: 144 MGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFNPAKACSRKLVGARYFI 203
           MG+GAIIG+LDTGIWPESEVFCD+GLGPVPSRWKGICE GELF+PAKACSRKL+GARYF+
Sbjct: 1   MGSGAIIGVLDTGIWPESEVFCDEGLGPVPSRWKGICESGELFSPAKACSRKLIGARYFV 60

Query: 204 KGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVGYHGLAVGTVRGGAPQ 263
           KGLEAAYGHP NN+ FQDYLSPRD SGHGTHVSSVA G FVPNV YHGLAVGTVRGGAP 
Sbjct: 61  KGLEAAYGHPLNNSEFQDYLSPRDRSGHGTHVSSVASGFFVPNVSYHGLAVGTVRGGAPH 120

Query: 264 SRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVLSLSLGPTFPSYSDVDMHDGIAIG 323
           SR+AMYKVCW++NGGVC+DIDILKAIDQAIYDGVDVLSLSLGP+FPSYSDVDM +GIAIG
Sbjct: 121 SRIAMYKVCWQVNGGVCADIDILKAIDQAIYDGVDVLSLSLGPSFPSYSDVDMRNGIAIG 180

Query: 324 AFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 383
           AFHAVAKGI VVGAAGNSGPAAYSV+NIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG
Sbjct: 181 AFHAVAKGIVVVGAAGNSGPAAYSVSNIEPWLLTVAASSVDRSFLVAITLGNNWTTMGQG 240

Query: 384 MFSGKLTRFHNLVYPEVSDLDDPRACESLSLNDTWAAGNVVLCFASDDYDDNTHDTSWSV 443
           MFSGKLT+F NLVYPEVSDLDD RACESLSLNDTWA+GNVVLCFASDDY+DNTH+TSWSV
Sbjct: 241 MFSGKLTKFINLVYPEVSDLDDERACESLSLNDTWASGNVVLCFASDDYNDNTHNTSWSV 300

Query: 444 KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQILNYIRSTRNPQVKIGPSTTRVG 503
           KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLD+GMQILNYIRSTRNPQVKIG STTRVG
Sbjct: 301 KKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDIGMQILNYIRSTRNPQVKIGASTTRVG 360

Query: 504 QSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKERNSYAFISGTSMATP 563
           Q LSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK RNSYAFISGTSMATP
Sbjct: 361 QPLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPKNRNSYAFISGTSMATP 420

Query: 564 HVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGEVIFAEGRPMKSAGPFDFGGGIVN 623
           HVSAIVALLK+LHSHWSPAAIKSAIVTTAW+SDPYG VIFAEGRPM SA PFDFGGGIVN
Sbjct: 421 HVSAIVALLKTLHSHWSPAAIKSAIVTTAWSSDPYGAVIFAEGRPMMSARPFDFGGGIVN 480

Query: 624 PNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSITCPKRRPSILDINVPSITIP 683
           PNKA+DPGLVYDMGMADYI+YFCA G+NNSAISGI+KRSI+CPKRRPSILDINVPSITIP
Sbjct: 481 PNKAVDPGLVYDMGMADYIQYFCAKGYNNSAISGISKRSISCPKRRPSILDINVPSITIP 540

Query: 684 SLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVKPRILKFNHKMERISFTVTVSTNH 743
           SL +SV+LTRTV NVG +NS+YKAAI  PPGI I VKPRILKFNHK + ISFTVT+S+N 
Sbjct: 541 SLTHSVSLTRTVTNVGAINSSYKAAIEVPPGITIAVKPRILKFNHKTKSISFTVTISSNQ 600

Query: 744 RVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           RVTTGYCFGSLTWLDG+HSVRIP+SVRTNI
Sbjct: 601 RVTTGYCFGSLTWLDGVHSVRIPVSVRTNI 630

BLAST of Lag0020167 vs. TAIR 10
Match: AT1G32960.1 (Subtilase family protein )

HSP 1 Score: 823.5 bits (2126), Expect = 1.4e-238
Identity = 418/773 (54.08%), Postives = 548/773 (70.89%), Query Frame = 0

Query: 8   KSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 67
           +S R++I LV +S  T LN        ++ +S V+IVY+G + H + E + ++HH +LAS
Sbjct: 2   RSFRSSILLVLLSLITVLN---ATRARSETESKVHIVYLGEKKHHDPEFVTESHHQMLAS 61

Query: 68  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 127
           +LGS++ + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  H++ TTR+W+Y
Sbjct: 62  LLGSKKDADDSMVYSYRHGFSGFAAKLTKSQAKKIADLPEVVHVIPDGFHELATTRTWEY 121

Query: 128 LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFN 187
           L LS   P +LL  + MG+  IIG++DTG+WPESE F D+G+GP+P +WKG CE GE F 
Sbjct: 122 LGLSSANPKNLLNDTNMGDQVIIGVIDTGVWPESESFNDNGVGPIPRKWKGGCESGENFR 181

Query: 188 PAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 247
            +  C+RKL+GA+YFI G   A    FN T  +DY+S RD+ GHGTHV+S+AGGSFVPNV
Sbjct: 182 -STDCNRKLIGAKYFINGF-LAENKGFNTTESRDYISARDFDGHGTHVASIAGGSFVPNV 241

Query: 248 GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 307
            Y GLA GT+RGGAP++R+AMYK CW    L G  CSD DI+KAID+AI+DGVDVLS+SL
Sbjct: 242 SYKGLAGGTLRGGAPRARIAMYKACWFHEELKGVTCSDSDIMKAIDEAIHDGVDVLSISL 301

Query: 308 GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 367
               P  S+ D+ D  A G FHAVAKGI VV A GN GPAA +V NI PW+LTVAA+++D
Sbjct: 302 VGQIPLNSETDIRDEFATGLFHAVAKGIVVVCAGGNDGPAAQTVVNIAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPR---ACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ  ++G      +LVYPE +  ++      CESL+LN  +  A
Sbjct: 362 RSFPTPITLGNNKVILGQATYTGPELGLTSLVYPENARNNNETFSGVCESLNLNPNYTMA 421

Query: 428 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 487
             VVLCF +   +      +  VK  GGLGLI+++NP   + P  D+FPCV +  ++G  
Sbjct: 422 MKVVLCFTASRTNAAISRAASFVKAAGGLGLIISRNPVYTLSPCNDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL+YIRSTR+P VKI  S T  GQ + T V  FSSRGPNS++PAILKPDIAAPGV ILAA
Sbjct: 482 ILSYIRSTRSPVVKIQRSRTLSGQPVGTKVVNFSSRGPNSMSPAILKPDIAAPGVRILAA 541

Query: 548 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 607
             P+D      +A +SGTSMATP +S ++ALLK+LH  WSPAA +SAIVTTAW +DP+GE
Sbjct: 542 TSPNDTLNVGGFAMLSGTSMATPVISGVIALLKALHPEWSPAAFRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 667
            IFAEG   K + PFD+GGGIVNP KA +PGL+YDMG  DYI Y C+ G+N+S+IS +  
Sbjct: 602 QIFAEGSSRKVSDPFDYGGGIVNPEKAAEPGLIYDMGPQDYILYLCSAGYNDSSISQLVG 661

Query: 668 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 727
           +   C   +PS+LD+N+PSITIP+L+  V LTRTV NVGLV+S YK ++ PP G+ + V 
Sbjct: 662 QITVCSNPKPSVLDVNLPSITIPNLKDEVTLTRTVTNVGLVDSVYKVSVEPPLGVRVVVT 721

Query: 728 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           P  L FN K   +SFTV VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLVFNSKTISVSFTVRVSTTHKINTGYYFGSLTWTDSVHNVVIPLSVRTQI 769

BLAST of Lag0020167 vs. TAIR 10
Match: AT4G10520.1 (Subtilase family protein )

HSP 1 Score: 815.8 bits (2106), Expect = 2.9e-236
Identity = 428/766 (55.87%), Postives = 549/766 (71.67%), Query Frame = 0

Query: 10  SRTTIFL-VFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASV 69
           S+T +FL +F+S    LN   + V+   A+S VY+VY+G + HDN E + ++HH +L S+
Sbjct: 2   SKTILFLALFLSI--VLNVQISFVV---AESKVYVVYLGEKEHDNPESVTESHHQMLWSL 61

Query: 70  LGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYL 129
           LGS+EA  DS+VY+Y++GFSGFAAKLT +QAQ +SELP V++VIPN L++M TTR+WDYL
Sbjct: 62  LGSKEAVLDSIVYSYRHGFSGFAAKLTESQAQQISELPEVVQVIPNTLYEMTTTRTWDYL 121

Query: 130 QLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFNP 189
            +SP   +SLLQK+ MG   I+G++D+G+WPESE+F D G GP+PSRWKG CE GELFN 
Sbjct: 122 GVSPGNSDSLLQKANMGYNVIVGVIDSGVWPESEMFNDKGFGPIPSRWKGGCESGELFNA 181

Query: 190 AKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVG 249
           +  C+RKL+GA+YF+ GL A +G   N T   +YLSPRD++GHGTHV+S  GGSF+PNV 
Sbjct: 182 SIHCNRKLIGAKYFVDGLVAEFG-VVNRTQNPEYLSPRDFAGHGTHVASTIGGSFLPNVS 241

Query: 250 YHGLAVGTVRGGAPQSRLAMYKVCWRLNGGVCSDIDILKAIDQAIYDGVDVLSLSLGPTF 309
           Y GL  GT RGGAP   +A+YK CW    G CS  D+LKA+D+AI+DGVD+LSLSLGP+ 
Sbjct: 242 YVGLGRGTARGGAPGVHIAVYKACW---SGYCSGADVLKAMDEAIHDGVDILSLSLGPSV 301

Query: 310 PSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSFL 369
           P + +    +  ++GAFHAVAKGI VV AAGN+GP A +++N+ PW+LTVAA++ DRSF 
Sbjct: 302 PLFPET---EHTSVGAFHAVAKGIPVVIAAGNAGPTAQTISNVAPWVLTVAATTQDRSFP 361

Query: 370 VAITLGNNWTTMGQGMFSGKLTRFHNLVYPEVSDLDDPRACESLSLN-DTWAAGNVVLCF 429
            AITLGNN T +GQ ++ G    F  L YPE     D   CE LS N ++   G VVLCF
Sbjct: 362 TAITLGNNITILGQAIYGGPELGFVGLTYPESPLSGD---CEKLSANPNSTMEGKVVLCF 421

Query: 430 ASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQILNYIRS 489
           A+      ++    +V   GGLGLI+AKNPT ++ P    FP V I  ++G  IL YIRS
Sbjct: 422 AA---STPSNAAIAAVINAGGLGLIMAKNPTHSLTP-TRKFPWVSIDFELGTDILFYIRS 481

Query: 490 TRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPPSDPK 549
           TR+P VKI  S T  GQS+ST VA FSSRGPNSV+PAILKPDIAAPGV ILAA+ P+   
Sbjct: 482 TRSPIVKIQASKTLFGQSVSTKVATFSSRGPNSVSPAILKPDIAAPGVNILAAISPNSSI 541

Query: 550 ERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGEVIFAEGR 609
               +A +SGTSMATP VS +V LLKSLH  WSP+AIKSAIVTTAW +DP GE IFA+G 
Sbjct: 542 NDGGFAMMSGTSMATPVVSGVVVLLKSLHPDWSPSAIKSAIVTTAWRTDPSGEPIFADGS 601

Query: 610 PMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSITCPK 669
             K A PFD+GGG++NP KA+ PGL+YDM   DY+ Y C++ +++ +IS +  +   CP 
Sbjct: 602 SRKLADPFDYGGGLINPEKAVKPGLIYDMTTDDYVMYMCSVDYSDISISRVLGKITVCPN 661

Query: 670 RRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVKPRILKFN 729
            +PS+LD+N+PSITIP+LR  V LTRTV NVG VNS YK  I PP GI + V P  L F+
Sbjct: 662 PKPSVLDLNLPSITIPNLRGEVTLTRTVTNVGPVNSVYKVVIDPPTGINVAVTPAELVFD 721

Query: 730 HKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           +   + SFTV VST H+V TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 YTTTKRSFTVRVSTTHKVNTGYYFGSLTWTDNMHNVAIPVSVRTQI 748

BLAST of Lag0020167 vs. TAIR 10
Match: AT4G10550.1 (Subtilase family protein )

HSP 1 Score: 815.1 bits (2104), Expect = 4.9e-236
Identity = 410/770 (53.25%), Postives = 545/770 (70.78%), Query Frame = 0

Query: 11  RTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLASVLG 70
           RT+I++V +S    LN   + V  + AK  V+IVY+G + HD+ E + ++HH +L S+LG
Sbjct: 5   RTSIYVV-LSLVIFLNVQRSFVAESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWSLLG 64

Query: 71  SQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDYLQL 130
           S+E + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  +K+ TTR+WDYL L
Sbjct: 65  SKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDYLGL 124

Query: 131 SPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFNPAK 190
           S   P SLL ++ MG   IIG++DTG+WPESEVF D G GPVPS WKG CE GE FN + 
Sbjct: 125 SAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFNSSN 184

Query: 191 ACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNVGYH 250
            C++KL+GA+YFI G   A    FN+T   D++SPRD  GHGTHVS++AGGSFVPN+ Y 
Sbjct: 185 -CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNISYK 244

Query: 251 GLAVGTVRGGAPQSRLAMYKVCWRLNGG---VCSDIDILKAIDQAIYDGVDVLSLSLGPT 310
           GLA GTVRGGAP++ +AMYK CW L+      CS  DILKA+D+A++DGVDVLS+SLG +
Sbjct: 245 GLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISLGSS 304

Query: 311 FPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVDRSF 370
            P Y + D+ DGI  GAFHAV KGI VV + GNSGP + +V N  PW++TVAA+++DRSF
Sbjct: 305 VPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLDRSF 364

Query: 371 LVAITLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAAGNV 430
              +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N +    G V
Sbjct: 365 ATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTMEGKV 424

Query: 431 VLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQILN 490
           VLCF +  Y       +  VK+ GGLG+I+A++P  A++P +D+FPCV +  ++G  IL 
Sbjct: 425 VLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTDILL 484

Query: 491 YIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAAVPP 550
           Y RS+ +P VKI PS T VGQ + T VA FSSRGPNS+APAILKPDIAAPGV+ILAA   
Sbjct: 485 YTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAATTN 544

Query: 551 SDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGEVIF 610
           +   ++  +  +SGTSMA P +S + ALLK+LH  WSPAAI+SAIVTTAW +DP+GE IF
Sbjct: 545 TTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGEQIF 604

Query: 611 AEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITKRSI 670
           AEG P K A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C++G+N ++IS +  ++ 
Sbjct: 605 AEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIGKTT 664

Query: 671 TCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVKPRI 730
            C   +PS+LD N+PSITIP+L+  V +TRTV NVG +NS Y+  + PP G  + V P  
Sbjct: 665 VCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVTPET 724

Query: 731 LKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           L FN   +++ F V VST H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 725 LVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 770

BLAST of Lag0020167 vs. TAIR 10
Match: AT4G10550.3 (Subtilase family protein )

HSP 1 Score: 813.9 bits (2101), Expect = 1.1e-235
Identity = 411/773 (53.17%), Postives = 542/773 (70.12%), Query Frame = 0

Query: 8   KSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 67
           KSS+ TIFL     F A          + AK  V+IVY+G + HD+ E + ++HH +L S
Sbjct: 26  KSSKETIFLTKERSFVA---------ESSAKRKVHIVYLGEKQHDDPEFVTESHHRMLWS 85

Query: 68  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 127
           +LGS+E + DS+VY+Y++GFSGFAAKLT +QA+ +++LP V+ VIP+  +K+ TTR+WDY
Sbjct: 86  LLGSKEDANDSMVYSYRHGFSGFAAKLTESQAKKIADLPDVVHVIPDSFYKLATTRTWDY 145

Query: 128 LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFN 187
           L LS   P SLL ++ MG   IIG++DTG+WPESEVF D G GPVPS WKG CE GE FN
Sbjct: 146 LGLSAANPKSLLHETNMGEQIIIGVIDTGVWPESEVFNDSGFGPVPSHWKGGCETGENFN 205

Query: 188 PAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 247
            +  C++KL+GA+YFI G   A    FN+T   D++SPRD  GHGTHVS++AGGSFVPN+
Sbjct: 206 SSN-CNKKLIGAKYFINGF-LAENESFNSTNSLDFISPRDLDGHGTHVSTIAGGSFVPNI 265

Query: 248 GYHGLAVGTVRGGAPQSRLAMYKVCWRLNGG---VCSDIDILKAIDQAIYDGVDVLSLSL 307
            Y GLA GTVRGGAP++ +AMYK CW L+      CS  DILKA+D+A++DGVDVLS+SL
Sbjct: 266 SYKGLAGGTVRGGAPRAHIAMYKACWYLDDDDTTTCSSADILKAMDEAMHDGVDVLSISL 325

Query: 308 GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 367
           G + P Y + D+ DGI  GAFHAV KGI VV + GNSGP + +V N  PW++TVAA+++D
Sbjct: 326 GSSVPLYGETDIRDGITTGAFHAVLKGITVVCSGGNSGPDSLTVTNTAPWIITVAATTLD 385

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLN-DTWAA 427
           RSF   +TLGNN   +GQ M++G    F +LVYPE    S+      CE L  N +    
Sbjct: 386 RSFATPLTLGNNKVILGQAMYTGPGLGFTSLVYPENPGNSNESFSGTCEELLFNSNRTME 445

Query: 428 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 487
           G VVLCF +  Y       +  VK+ GGLG+I+A++P  A++P +D+FPCV +  ++G  
Sbjct: 446 GKVVLCFTTSPYGGAVLSAARYVKRAGGLGVIIARHPGYAIQPCLDDFPCVAVDWELGTD 505

Query: 488 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL Y RS+ +P VKI PS T VGQ + T VA FSSRGPNS+APAILKPDIAAPGV+ILAA
Sbjct: 506 ILLYTRSSGSPVVKIQPSKTLVGQPVGTKVATFSSRGPNSIAPAILKPDIAAPGVSILAA 565

Query: 548 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 607
              +   ++  +  +SGTSMA P +S + ALLK+LH  WSPAAI+SAIVTTAW +DP+GE
Sbjct: 566 TTNTTFSDQ-GFIMLSGTSMAAPAISGVAALLKALHRDWSPAAIRSAIVTTAWKTDPFGE 625

Query: 608 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 667
            IFAEG P K A PFD+GGG+VNP K+ +PGLVYDMG+ DY+ Y C++G+N ++IS +  
Sbjct: 626 QIFAEGSPPKLADPFDYGGGLVNPEKSANPGLVYDMGLEDYVLYMCSVGYNETSISQLIG 685

Query: 668 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 727
           ++  C   +PS+LD N+PSITIP+L+  V +TRTV NVG +NS Y+  + PP G  + V 
Sbjct: 686 KTTVCSNPKPSVLDFNLPSITIPNLKDEVTITRTVTNVGPLNSVYRVTVEPPLGFQVTVT 745

Query: 728 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           P  L FN   +++ F V VST H+  TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 746 PETLVFNSTTKKVYFKVKVSTTHKTNTGYYFGSLTWSDSLHNVTIPLSVRTQI 786

BLAST of Lag0020167 vs. TAIR 10
Match: AT4G10540.1 (Subtilase family protein )

HSP 1 Score: 812.4 bits (2097), Expect = 3.2e-235
Identity = 417/773 (53.95%), Postives = 541/773 (69.99%), Query Frame = 0

Query: 8   KSSRTTIFLVFISFFTALNDLCTLVITADAKSNVYIVYMGNRPHDNEELLVKTHHGVLAS 67
           KS RT IF+  I     LN L T V  A A+S V+IVY+G + HD+ E + ++HH +L S
Sbjct: 2   KSCRTLIFVAII-----LNGLSTFVAHAGAESKVHIVYLGEKQHDDPEFVTESHHRMLWS 61

Query: 68  VLGSQEASADSLVYNYKYGFSGFAAKLTMAQAQMMSELPSVIKVIPNRLHKMQTTRSWDY 127
           +LGS+E +  S+V++Y++GFSGFAAKLT +QA+ +++LP V+ V P+  +++ TTR+WDY
Sbjct: 62  LLGSKEDAHSSMVHSYRHGFSGFAAKLTKSQAKKLADLPEVVHVTPDSFYQLDTTRTWDY 121

Query: 128 LQLSPRFPNSLLQKSRMGNGAIIGLLDTGIWPESEVFCDDGLGPVPSRWKGICEPGELFN 187
           L LS   P +LL  + MG   IIG++D+G+WPESEVF D+G+GPVPS WKG C  GE F 
Sbjct: 122 LGLSVANPKNLLNDTNMGEEVIIGIVDSGVWPESEVFNDNGIGPVPSHWKGGCVSGENFT 181

Query: 188 PAKACSRKLVGARYFIKGLEAAYGHPFNNTGFQDYLSPRDYSGHGTHVSSVAGGSFVPNV 247
            ++ C++KL+GA+YFI G  A +   FN+T   D++SPRD SGHGTHV+++AGGS+VP++
Sbjct: 182 SSQ-CNKKLIGAKYFINGFLATH-ESFNSTESLDFISPRDRSGHGTHVATIAGGSYVPSI 241

Query: 248 GYHGLAVGTVRGGAPQSRLAMYKVCW---RLNGGVCSDIDILKAIDQAIYDGVDVLSLSL 307
            Y GLA GTVRGGAP++R+AMYK CW   R +   CS  DILKA+D+A++DGVDVLSLS+
Sbjct: 242 SYKGLAGGTVRGGAPRARIAMYKACWYLDRFDINTCSSADILKAMDEAMHDGVDVLSLSI 301

Query: 308 GPTFPSYSDVDMHDGIAIGAFHAVAKGIAVVGAAGNSGPAAYSVANIEPWLLTVAASSVD 367
           G  FP + + D+   IA GAFHAV KGI VV + GNSGPAA +V N  PW+LTVAA+++D
Sbjct: 302 GYRFPYFPETDVRAVIATGAFHAVLKGITVVCSGGNSGPAAQTVGNTAPWILTVAATTLD 361

Query: 368 RSFLVAITLGNNWTTMGQGMFSGKLTRFHNLVYPE---VSDLDDPRACESLSLNDTWA-A 427
           RSF   ITLGNN   +GQ M++G    F +LVYPE    S+      CE L  N     A
Sbjct: 362 RSFPTPITLGNNKLILGQAMYTGPELGFTSLVYPENPGNSNESFSGDCELLFFNSNHTMA 421

Query: 428 GNVVLCFASDDYDDNTHDTSWSVKKVGGLGLIVAKNPTKAVEPFIDNFPCVQISLDVGMQ 487
           G VVLCF +             VK+ GGLG+IVA+NP   + P  D+FPCV +  ++G  
Sbjct: 422 GKVVLCFTTSTRYITVSSAVSYVKEAGGLGVIVARNPGDNLSPCEDDFPCVAVDYELGTD 481

Query: 488 ILNYIRSTRNPQVKIGPSTTRVGQSLSTTVAYFSSRGPNSVAPAILKPDIAAPGVAILAA 547
           IL YIRST  P VKI PS T VGQ + T VA FSSRGPNS+ PAILKPDIAAPGV+ILAA
Sbjct: 482 ILLYIRSTGLPVVKIQPSKTLVGQPVGTKVADFSSRGPNSIEPAILKPDIAAPGVSILAA 541

Query: 548 VPPSDPKERNSYAFISGTSMATPHVSAIVALLKSLHSHWSPAAIKSAIVTTAWTSDPYGE 607
              +       + F+SGTSMA P +S +VALLK+LH  WSPAAI+SAIVTTAW +DP+GE
Sbjct: 542 TTTNKTFNDRGFIFLSGTSMAAPTISGVVALLKALHRDWSPAAIRSAIVTTAWRTDPFGE 601

Query: 608 VIFAEGRPMKSAGPFDFGGGIVNPNKALDPGLVYDMGMADYIKYFCAMGHNNSAISGITK 667
            IFAEG P K A PFD+GGG+VNP KA  PGLVYD+G+ DY+ Y C++G+N ++IS +  
Sbjct: 602 QIFAEGSPRKLADPFDYGGGLVNPEKAAKPGLVYDLGLEDYVLYMCSVGYNETSISQLVG 661

Query: 668 RSITCPKRRPSILDINVPSITIPSLRYSVNLTRTVMNVGLVNSTYKAAIVPPPGIAIFVK 727
           +   C   +PS+LD N+PSITIP+L+  V LTRT+ NVG + S YK  I PP GI + V 
Sbjct: 662 KGTVCSNPKPSVLDFNLPSITIPNLKDEVTLTRTLTNVGQLESVYKVVIEPPIGIQVTVT 721

Query: 728 PRILKFNHKMERISFTVTVSTNHRVTTGYCFGSLTWLDGIHSVRIPISVRTNI 774
           P  L FN   +R+SF V VST H++ TGY FGSLTW D +H+V IP+SVRT I
Sbjct: 722 PETLLFNSTTKRVSFKVKVSTTHKINTGYFFGSLTWSDSLHNVTIPLSVRTQI 767