Lag0020056 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0020056
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionbeta-carotene hydroxylase 2, chloroplastic-like
Locationchr5: 47731516 .. 47734305 (-)
RNA-Seq ExpressionLag0020056
SyntenyLag0020056
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTAACTTCCTCCAATCTCTCTCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCCTCCGGAGCAGTAGAATCCAATGGAAGATGCGGAGAAAACCGCTGCTCACCGTCTGTGTGCTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCAATGAAGGATCGCCGATTGTAGTCCCTCAGATCCCGTCGCCTCATGTTGCAGAGAAATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCCGCGGTGATGTCTAGTTTTGGAATTACTTCCATGGCTATCATGGCTGTTTACTACCGATTTTACTGGCAAATGGAGGTCCTCTCTCTCTCTCTCTCTAGAAACTTGAACTATTTTTTTTTCATTGGCGCCGTATTCTTCTGACTGAATATTAAATTGCGATTGTTTAATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCAGAAATGTTCGGTACATTTTCTCTATCCGTTGGTGCCGCTGTAAGTTTTCAATTTCCTTTAATCTCTCTATCTCTACTTTTCTCGAAGAATTCGATTTGCTATAAGCTGATTTTTCACAATCAATTTGGGATTATCAGGTCGGCATGGAGTTTTGGGCAAGATGGGCGCATAGGGCACTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTATAGGTTTCTAATCCTTCTTCTCTATAGAATTTCGTTTGTGTTCGTCTGCTGAGAAAATTTCAATCAACAATAATCCACCGCGAAACTTAATTTCATAGCAAACAAAACAGTATTCAATGGAAAAAAAGAATCGTTGACGCTTGGATTTGAAGATTGCCGACCAGTCGCACCATTCCTAAACTAATCCTCTTCTTTAGAATTTTCTTTGTCTGGCTGATGATTAAATTCAATTAACAATCGTCCGCTGTGGACCTTTCCTCTCATTTTCCATGTTCACTTTCGTAGCAAACAAACAACACCGAATTAAAAAAGAAAATTCGTTGACGCCTGGACTTTGTTGATCTTGATTATTACAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTTCTCTCTTACGGGTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCTATCACTTTCTCCCTCATAATCTTTTAGAATCTCAGTTTGTTCATATTCCAACTGTTTAATGGTTATTTAATCACTGGGGATTAGCGATAATTACTGTATTAAATGACGAAAATAGCCCTGAAAAGTTTTGGTAATGGAAAATAGTTTGTCGCATATACCTAGGTATGTAGGTAGGATCTCATTGCACCATCAAAATGTTGACAATCAACTTTGTCTTTTTAGTTGTCAAGATTTAATAGAATATATAAAAATTTTCGAGCATACGGATGGGATCACACTACCATTAAAATGTTGACAATCTGTATTTCGTTTTAGTTGTCAAGATTTTAATAAAATATACAGGTGGGATCACATTGCATACTCACATCGCATACCACGATTAAAATGTTGACAATCAGTTTTCTCTATTTTAATTGTCAAGATTTTAACAGAGTATATAAAAATCTTGGTGCATATTAGTGTACAGTCGTTGTTTGCTTTATTAAAAAAAACAAGGTATTATTCTTTGCTAATTGAGAAAGTTTTGACACGCTGGCGGTCTGTGGCAGGGCCTTGGAATTACAGTGTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTTGCTGCTGCTCACAAGGTAAATTCAATTTCTAAATATTAATTTCCTTTTTTTAAATGCTTATTGCTTAATCTTTAAAGAAAAGCAAATTTGTTATTATTTAAATCTTATTTTGATCTCAAAATCTTGATACTGTCTCTCTTTACTCTCTATTGTGTTGACTTTTCATTAATGATTTTAACAAAAACTCAATTATGTTTCAAACCTTGATATTTGGATCGCTTAATATACGGAAAAAATAGGGTTTTTAATGTCTTTTTTTTCCCCTTAGAATATTGAGAAACGTATGGATCTCGACCAAAGAATATAAAAACTTAGGTCTATCAAATGAGTCTTATAAATAAAAATATGATTTAAAATCAATCGACTAATAATATATCAGTAATAATTGAACCAAGAAAATAATGAAAATATAATAATATGCTTAGACGACTTCGTGCAATTTTTAATAAACTCATATTAGATTATGTTTTTTTACTGTTAAAATTAAAAACGTGTTATTAACTTGCATATAGAAAAGTAATTACATCTTTCTCAAAAAAAAAAAAAGAAAAGAAAAGTAATTACACGTGTCTTAAGTGTAAATCTAAATTTAAAACCATTTAAATATTGAAGCTTATGATTTTTTCTTCTTCTTCTTGGCTGTAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTTAGTTTCGGGTCTATTTTTTTATTTATTTTTAAATCTTAAAATTTAAATTTATTATTTAAAAAAATTTTAATAAATGGCTTTCCTAAAAATGGTGTGGTGCAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCTTGA

mRNA sequence

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTAACTTCCTCCAATCTCTCTCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCCTCCGGAGCAGTAGAATCCAATGGAAGATGCGGAGAAAACCGCTGCTCACCGTCTGTGTGCTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCAATGAAGGATCGCCGATTGTAGTCCCTCAGATCCCGTCGCCTCATGTTGCAGAGAAATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCCGCGGTGATGTCTAGTTTTGGAATTACTTCCATGGCTATCATGGCTGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCAGAAATGTTCGGTACATTTTCTCTATCCGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCAAGATGGGCGCATAGGGCACTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTTCTCTCTTACGGGTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACAGTGTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTTGCTGCTGCTCACAAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCTTGA

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCACTCCATCTCTTCCTAACTTCCTCCAATCTCTCTCCTAAACCTCGAACTCCGTTTCTGTTTCCACCTTCAGTCCTCCGGAGCAGTAGAATCCAATGGAAGATGCGGAGAAAACCGCTGCTCACCGTCTGTGTGCTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCAATGAAGGATCGCCGATTGTAGTCCCTCAGATCCCGTCGCCTCATGTTGCAGAGAAATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCCGCGGTGATGTCTAGTTTTGGAATTACTTCCATGGCTATCATGGCTGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCAGAAATGTTCGGTACATTTTCTCTATCCGTTGGTGCCGCTGTCGGCATGGAGTTTTGGGCAAGATGGGCGCATAGGGCACTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCGCACCATAAACCAAGAGAAGGACCGTTCGAACTGAACGATGTCTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTTCTCTCTTACGGGTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACAGTGTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCCTGTGGGTCCCATTGCCAACGTGCCCTATTTCAGAAAGGTTGCTGCTGCTCACAAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAATTAGAGGAAGTGGGAGGCTTAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCAACGGTTCTTGA

Protein sequence

MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVEDQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKSTATKSINGS
Homology
[Close]
BLAST of Lag0020056 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 588.6 bits (1516), Expect = 3.2e-164
Identity = 287/310 (92.58%), Postives = 300/310 (96.77%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAAGLSAAL PKPLHL LTSS+LSPKPR PFLFPPSV R+SRIQWKMRR+ L TVCVLVE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQNSSGEVENL++E SPI+VPQIPSPHV+E+LARK+SERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSINGS 311
             A KSI+GS
Sbjct: 301 WAAPKSIDGS 310

[Close]
BLAST of Lag0020056 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 588.6 bits (1516), Expect = 3.2e-164
Identity = 287/310 (92.58%), Postives = 299/310 (96.45%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAAGLSA LGPKP H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRK LLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQ+SS EVENLA+E  PI+VPQIPSPHVAEKLARK+SERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHS+KFNGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STATKSINGS 311
           STA K INGS
Sbjct: 301 STAKKPINGS 310

[Close]
BLAST of Lag0020056 vs. NCBI nr
Match: XP_023541483.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.8 bits (1514), Expect = 5.5e-164
Identity = 286/310 (92.26%), Postives = 298/310 (96.13%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAA +SA LGPKP H+FLTSSNLSPKPRTPFL+P SV RSSRIQWKMRRK LLTVCVLVE
Sbjct: 1   MAASVSAVLGPKPFHVFLTSSNLSPKPRTPFLYPSSVFRSSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQ+SS EVENLA+E  PI+VPQIPSPHVAEKLARK+SERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQSSSSEVENLADENLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHS+KFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSINGS 311
           STA K INGS
Sbjct: 301 STAKKPINGS 310

[Close]
BLAST of Lag0020056 vs. NCBI nr
Match: XP_022944865.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 587.4 bits (1513), Expect = 7.1e-164
Identity = 286/310 (92.26%), Postives = 298/310 (96.13%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAAGLSA LGPKP H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRK LLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQ+SS EVENLA+E  PI+VPQIPSPHVAEKLARK+SERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHS+KFNGVPYGLFLGPKELEEVGGL+ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STATKSINGS 311
           STA K INGS
Sbjct: 301 STAKKPINGS 310

[Close]
BLAST of Lag0020056 vs. NCBI nr
Match: KAG6574199.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 585.5 bits (1508), Expect = 2.7e-163
Identity = 286/310 (92.26%), Postives = 298/310 (96.13%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAAGLSA LGPK  H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRK LLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKSFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQ+SS EVENLA+E  PI+VPQIPSPHVAEKLARK+SERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHS+KFNGVPYGLFLGPKELEEVGGLEELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STATKSINGS 311
           STA K INGS
Sbjct: 301 STAKKPINGS 310

[Close]
BLAST of Lag0020056 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 444.5 bits (1142), Expect = 9.8e-124
Identity = 229/308 (74.35%), Postives = 249/308 (80.84%), Query Frame = 0 

Query: 6   SAALGPKPLHLFLTSSNLSPKPRTPFLFPP---SVLRSSRIQWKMRRKPLLTVCVLVEDQ 65
           S  LGPKP     T +  S  P TP  F P   S+LR        RR+P  TVC ++ED 
Sbjct: 20  SPFLGPKP-----TPTTPSVYPITP--FSPNLGSILR-------CRRRPSFTVCFVLEDD 79

Query: 66  NSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMAVY 125
               + E    +    +  QI +  +AEKLARK+SERFTYLVAAVMSSFGITSMA+MAVY
Sbjct: 80  KFKTQFEAGEEDIEMKIEEQISATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVY 139

Query: 126 YRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREG 185
           YRFYWQMEGGE+PFSEMFGTF+LSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREG
Sbjct: 140 YRFYWQMEGGEVPFSEMFGTFALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREG 199

Query: 186 PFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRF 245
           PFELNDVFAIINAVPAIALL YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF
Sbjct: 200 PFELNDVFAIINAVPAIALLDYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRF 259

Query: 246 PVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKST 305
           PVGP+ANVPY RKVAAAH LHHS+KFNGVPYGLFLGPKELEEVGGLEELEKE+NRR    
Sbjct: 260 PVGPVANVPYLRKVAAAHSLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRR---- 308

Query: 306 ATKSINGS 311
            T+ I GS
Sbjct: 320 -TRYIKGS 308

[Close]
BLAST of Lag0020056 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 428.3 bits (1100), Expect = 7.3e-119
Identity = 220/299 (73.58%), Postives = 249/299 (83.28%), Query Frame = 0 

Query: 18  LTSSNLSPK-----PRTPFL--FPPSVLRSSRIQWKMRRKPLLTVCVLVEDQNSSGE--V 77
           ++SS+L+PK       TP L  FPP  L S  +  + R KP LTVC ++E++   G+  V
Sbjct: 21  ISSSSLTPKSSPVSTSTPTLVVFPPFKLVSKSL--RTRSKPRLTVCFVLEEKELRGKLVV 80

Query: 78  ENLANEGSPIVVPQ------IPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMAVY 137
            +  ++G+  V  Q        +  +A+KLARK+SERFTYLVAAVMSSFGITSMA+++VY
Sbjct: 81  ASDDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVY 140

Query: 138 YRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREG 197
           YRF WQMEGGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWHASLWHMHESHHKPREG
Sbjct: 141 YRFSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREG 200

Query: 198 PFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRF 257
           PFELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF
Sbjct: 201 PFELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRF 260

Query: 258 PVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIKS 302
           PVGPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPKELEEVGGL+ LE EINRR K+
Sbjct: 261 PVGPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRTKN 317

[Close]
BLAST of Lag0020056 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 416.4 bits (1069), Expect = 2.9e-115
Identity = 220/317 (69.40%), Postives = 249/317 (78.55%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFL---FPPSVLRSSRIQWKMRRKPLLTVCV 60
           MAAGLS A+  KPLH   +SS+   + R P     F PS LR  R           +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPS-LRFKR----------FSVCY 60

Query: 61  LVEDQNSSGEVENLANEGSPIVVPQIPSPH----VAEKLARKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +EN     S      I + +    +AEKL RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAIMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMH 180
           TSMA+MAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEK 300
           HDGLVHKRFPVGPIA+VPY RKVAAAH+LHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 301 EINRRIKSTATKSINGS 311
           EI+RRIKS    S +GS
Sbjct: 301 EISRRIKSYKKASGSGS 306

[Close]
BLAST of Lag0020056 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 409.1 bits (1050), Expect = 4.6e-113
Identity = 211/315 (66.98%), Postives = 246/315 (78.10%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPR-----TPFLFPPSVLRSSRIQWKMRRKPLLTV 60
           MAA +S +   + + L     N  P P+      P LF   +  +     + RRKP L  
Sbjct: 1   MAAEISISASSRAICL---QRNPFPAPKYFATAPPLLFFSPLTCNLDAILRSRRKPRLAA 60

Query: 61  CVLVEDQN----SSGEVENLANEGSPIVV----PQIPSPHVAEKLARKRSERFTYLVAAV 120
           C +++D       SG+  +    G  I V     +  +  +AEK ARK+SERFTYLVAAV
Sbjct: 61  CFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEEKSLAVRLAEKFARKKSERFTYLVAAV 120

Query: 121 MSSFGITSMAIMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHA 180
           MSS GITSMA+++VYYRF WQMEGGE+PFSEMF TF+L+ GAA+GME+WARWAHRALWHA
Sbjct: 121 MSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFCTFALAFGAAIGMEYWARWAHRALWHA 180

Query: 181 SLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFG 240
           SLWHMHESHH+PREGPFELND+FAIINAVPAIALLS+GF HKGL+PGLCFGAGLGITVFG
Sbjct: 181 SLWHMHESHHRPREGPFELNDIFAIINAVPAIALLSFGFNHKGLIPGLCFGAGLGITVFG 240

Query: 241 MAYMFVHDGLVHKRFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGG 300
           MAYMFVHDGLVHKRFPVGPIANVPYF++VAAAH+LHHSDKF+GVPYGLFLGPKELEEVG 
Sbjct: 241 MAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQLHHSDKFDGVPYGLFLGPKELEEVGV 300

Query: 301 LEELEKEINRRIKST 303
           LEELEKE+NRRIKS+
Sbjct: 301 LEELEKEVNRRIKSS 312

[Close]
BLAST of Lag0020056 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 1.1e-111
Identity = 210/302 (69.54%), Postives = 242/302 (80.13%), Query Frame = 0 

Query: 1   MAAGLSA-ALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLV 60
           MAAGLS  A+  KPL+    S+N    P +  +FPPS+  +       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLANEGSPIVVPQI-PSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAI 120
           E++  S  +++     S     +I  +  + +K  +K+SERFTYL+AAVMSSFGITSMAI
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 300
           HKRFPVGPIANVPY RKVAAAH+LHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

[Close]
BLAST of Lag0020056 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 587.4 bits (1513), Expect = 3.5e-164
Identity = 286/310 (92.26%), Postives = 298/310 (96.13%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAAGLSA LGPKP H+FLTSSNLSPKPRTPFLFP SV R+SRIQWKMRRK LLTVCVLVE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQ+SS EVENLA+E  PI+VPQIPSPHVAEKLARK+SERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHS+KFNGVPYGLFLGPKELEEVGGL+ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STATKSINGS 311
           STA K INGS
Sbjct: 301 STAKKPINGS 310

[Close]
BLAST of Lag0020056 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 286/310 (92.26%), Postives = 297/310 (95.81%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAA LSAALGPKPLHLFL SS+LS KPRTPFLFPPSV R+SRIQ KMRRK L TVCVLVE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQNSSGEVENL++E  PIVVPQIPSP+VA++LARKRSERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWH+S+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 STATKSINGS 311
            T TKSINGS
Sbjct: 301 LTTTKSINGS 310

[Close]
BLAST of Lag0020056 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 582.0 bits (1499), Expect = 1.5e-162
Identity = 283/310 (91.29%), Postives = 299/310 (96.45%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAAGLSAA  PKP HLFLTSS+LSPKP +PFLFPPSV R+SR QWKMRRK LLTVCVLVE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
            QNSSG+V+NL++E SPIVVPQIPSPHV+E+LARK+SERFTYLVAAVMS+FGITSMA+MA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSINGS 311
            TA KSI+GS
Sbjct: 301 WTARKSIDGS 310

[Close]
BLAST of Lag0020056 vs. ExPASy TrEMBL
Match: A0A6J1HX82 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.2e-162
Identity = 284/310 (91.61%), Postives = 295/310 (95.16%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAA LSA LGPKP H+FLTSSNLSPKPRTP LFP SV R+SRIQWKMRRK LLTVCVLVE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQ+SS EVENLA+E   I+VPQIPSPHVAEKLARK+SERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHS+K NGVPYGLFLGPKELEEVGGLEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSINGS 311
           STA K INGS
Sbjct: 301 STAKKPINGS 310

[Close]
BLAST of Lag0020056 vs. ExPASy TrEMBL
Match: A0A6J1E4L5 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430578 PE=3 SV=1)

HSP 1 Score: 579.7 bits (1493), Expect = 7.2e-162
Identity = 283/310 (91.29%), Postives = 298/310 (96.13%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLVE 60
           MAA +S ALGP+PLHLFL SS+LSPKPRTPFLFPPSV R+SRIQ K+RRK L TVCVLVE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLANEGSPIVVPQIPSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAIMA 120
           DQNSSGEVE+L++E  PIVVPQIPSP+VA++LARKRSERFTYLVAAVMSSFGITSMA+MA
Sbjct: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300
           RFPVGPIANVPYFRKVAAAH+LHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 STATKSINGS 311
            T TKSI+GS
Sbjct: 301 LTTTKSIDGS 310

[Close]
BLAST of Lag0020056 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 416.4 bits (1069), Expect = 2.0e-116
Identity = 220/317 (69.40%), Postives = 249/317 (78.55%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFL---FPPSVLRSSRIQWKMRRKPLLTVCV 60
           MAAGLS A+  KPLH   +SS+   + R P     F PS LR  R           +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPS-LRFKR----------FSVCY 60

Query: 61  LVEDQNSSGEVENLANEGSPIVVPQIPSPH----VAEKLARKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +EN     S      I + +    +AEKL RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAIMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMH 180
           TSMA+MAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFV 240
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFV
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFV 240

Query: 241 HDGLVHKRFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEK 300
           HDGLVHKRFPVGPIA+VPY RKVAAAH+LHH+DKFNGVPYGLFLGPKELEEVGG EEL+K
Sbjct: 241 HDGLVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDK 300

Query: 301 EINRRIKSTATKSINGS 311
           EI+RRIKS    S +GS
Sbjct: 301 EISRRIKSYKKASGSGS 306

[Close]
BLAST of Lag0020056 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 404.4 bits (1038), Expect = 8.0e-113
Identity = 210/302 (69.54%), Postives = 242/302 (80.13%), Query Frame = 0 

Query: 1   MAAGLSA-ALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLV 60
           MAAGLS  A+  KPL+    S+N    P +  +FPPS+  +       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLANEGSPIVVPQI-PSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAI 120
           E++  S  +++     S     +I  +  + +K  +K+SERFTYL+AAVMSSFGITSMAI
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHESHHK
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHESHHK 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
           PREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHDGLV
Sbjct: 181 PREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHDGLV 240

Query: 241 HKRFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 300
           HKRFPVGPIANVPY RKVAAAH+LHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 294

[Close]
BLAST of Lag0020056 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 260.4 bits (664), Expect = 1.9e-69
Identity = 143/226 (63.27%), Postives = 165/226 (73.01%), Query Frame = 0 

Query: 1   MAAGLSAALGPKPLHLFLTSSNLSPKPRTPFL---FPPSVLRSSRIQWKMRRKPLLTVCV 60
           MAAGLS A+  KPLH   +SS+   + R P     F PS LR  R           +VC 
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLRLPKSLSGFSPS-LRFKR----------FSVCY 60

Query: 61  LVEDQNSSGEVENLANEGSPIVVPQIPSPH----VAEKLARKRSERFTYLVAAVMSSFGI 120
           +VE++  +  +EN     S      I + +    +AEKL RK+SER TYL+AA++SSFGI
Sbjct: 61  VVEERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGI 120

Query: 121 TSMAIMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMH 180
           TSMA+MAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MH
Sbjct: 121 TSMAVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMH 180

Query: 181 ESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           ESHHKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 ESHHKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]
BLAST of Lag0020056 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 243.0 bits (619), Expect = 3.1e-64
Identity = 149/302 (49.34%), Postives = 179/302 (59.27%), Query Frame = 0 

Query: 1   MAAGLSA-ALGPKPLHLFLTSSNLSPKPRTPFLFPPSVLRSSRIQWKMRRKPLLTVCVLV 60
           MAAGLS  A+  KPL+    S+N    P +  +FPPS+  +       RR+ +LTVC +V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN---HPISTAVFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLANEGSPIVVPQI-PSPHVAEKLARKRSERFTYLVAAVMSSFGITSMAI 120
           E++  S  +++     S     +I  +  + +K  +K+SERFTYL+AAVMSSFGITSMAI
Sbjct: 61  EERKQSSPMDDDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITSMAI 120

Query: 121 MAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHK 180
           MAVYYRF WQM+GGE+   EMFGTF+LSVGA                             
Sbjct: 121 MAVYYRFSWQMKGGEVSVLEMFGTFALSVGA----------------------------- 180

Query: 181 PREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLV 240
                                                   AGLGIT+FGMAYMFVHDGLV
Sbjct: 181 ----------------------------------------AGLGITMFGMAYMFVHDGLV 225

Query: 241 HKRFPVGPIANVPYFRKVAAAHKLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRR 300
           HKRFPVGPIANVPY RKVAAAH+LHH+DKF GVPYGLFLGPKE+EEVGG EELEKEI+RR
Sbjct: 241 HKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEISRR 225

The following BLAST results are available for this feature:
BLAST of Lag0020056 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038877448.13.2e-16492.58beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida][more]
KAG7013258.13.2e-16492.58Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su... [more]
XP_023541483.15.5e-16492.26beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp... [more]
XP_022944865.17.1e-16492.26beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata][more]
KAG6574199.12.7e-16392.26Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su... [more]
BLAST of Lag0020056 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
O498149.8e-12474.35Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=... [more]
B3SGL07.3e-11973.58Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=... [more]
Q9SZZ82.9e-11569.40Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
O498154.6e-11366.98Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=... [more]
Q9LTG01.1e-11169.54Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=... [more]
BLAST of Lag0020056 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1FZ943.5e-16492.26beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata... [more]
A0A6J1J1S81.1e-16292.26beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A0A0L6061.5e-16291.29Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C... [more]
A0A6J1HX827.2e-16291.61beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima O... [more]
A0A6J1E4L57.2e-16291.29beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN... [more]
BLAST of Lag0020056 vs. TAIR 10
Match NameE-valueIdentityDescription
AT4G25700.12.0e-11669.40beta-hydroxylase 1 [more]
AT5G52570.18.0e-11369.54beta-carotene hydroxylase 2 [more]
AT4G25700.21.9e-6963.27beta-hydroxylase 1 [more]
AT5G52570.23.1e-6449.34beta-carotene hydroxylase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006694Fatty acid hydroxylasePFAMPF04116FA_hydroxylasecoord: 149..276
e-value: 1.3E-13
score: 51.5
IPR045019Beta-carotene hydroxylase-likePANTHERPTHR31899BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTICcoord: 16..301
NoneNo IPR availablePANTHERPTHR31899:SF10BETA-CAROTENE HYDROXYLASEcoord: 16..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0020056.1Lag0020056.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008610 lipid biosynthetic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016491 oxidoreductase activity