Lag0019918 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0019918
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPlant invertase/pectin methylesterase inhibitor superfamily protein
Locationchr5: 46708608 .. 46709201 (-)
RNA-Seq ExpressionLag0019918
SyntenyLag0019918
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGAAATCCGCCTTCCTTCTCCTTCTCCTCTCCATTTTCACCATCGCCGCCACCACCACCACCGCCGCCCGCAAAGGCGGAGCTTCCAATTTCATCAGATCCAGGTGCAGCGCCACAACTTACCCTGACCTCTGCTACCAATCCCTCTCAAATTTCGCCTCAACAATCCAGCAAAACCCTCGCCAATTAGTCCAAACCGCTCTCGTCGTCAGCCTCTCCCGCGCTCAATCCGCCCGATCCTTCGTCTGGAAACTCACCAAGTTCACCGGATTGAAGCCGAGAGAGTACGCCGCCCTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTGGATCGGCTGAGCAAATCCGTGGAAGAACTGAAGCGCGTCAGCGGATCGAAGCAGAAGGACTTCCTGTGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCCGCCATGACCGACGAGAACACTTGCTCCGATGGATTCGCCGGCACGGCGCTGAATGGGAGAGTCAAGTCCTCGGTTAGGGCTCGCATTGTGGATGTTACTCGCGTCATAAGCAATGCGTTGGCTCTGATTAACGAGTACGCTGAAAATCAAAAAAGCTAG

mRNA sequence

ATGGCGAAATCCGCCTTCCTTCTCCTTCTCCTCTCCATTTTCACCATCGCCGCCACCACCACCACCGCCGCCCGCAAAGGCGGAGCTTCCAATTTCATCAGATCCAGGTGCAGCGCCACAACTTACCCTGACCTCTGCTACCAATCCCTCTCAAATTTCGCCTCAACAATCCAGCAAAACCCTCGCCAATTAGTCCAAACCGCTCTCGTCGTCAGCCTCTCCCGCGCTCAATCCGCCCGATCCTTCGTCTGGAAACTCACCAAGTTCACCGGATTGAAGCCGAGAGAGTACGCCGCCCTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTGGATCGGCTGAGCAAATCCGTGGAAGAACTGAAGCGCGTCAGCGGATCGAAGCAGAAGGACTTCCTGTGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCCGCCATGACCGACGAGAACACTTGCTCCGATGGATTCGCCGGCACGGCGCTGAATGGGAGAGTCAAGTCCTCGGTTAGGGCTCGCATTGTGGATGTTACTCGCGTCATAAGCAATGCGTTGGCTCTGATTAACGAGTACGCTGAAAATCAAAAAAGCTAG

Coding sequence (CDS)

ATGGCGAAATCCGCCTTCCTTCTCCTTCTCCTCTCCATTTTCACCATCGCCGCCACCACCACCACCGCCGCCCGCAAAGGCGGAGCTTCCAATTTCATCAGATCCAGGTGCAGCGCCACAACTTACCCTGACCTCTGCTACCAATCCCTCTCAAATTTCGCCTCAACAATCCAGCAAAACCCTCGCCAATTAGTCCAAACCGCTCTCGTCGTCAGCCTCTCCCGCGCTCAATCCGCCCGATCCTTCGTCTGGAAACTCACCAAGTTCACCGGATTGAAGCCGAGAGAGTACGCCGCCCTGAAGGACTGTATGGAGGAGGTCGGCGACACCGTGGATCGGCTGAGCAAATCCGTGGAAGAACTGAAGCGCGTCAGCGGATCGAAGCAGAAGGACTTCCTGTGGCACATTAGCAACGTGGAGACTTGGGTGAGCGCCGCCATGACCGACGAGAACACTTGCTCCGATGGATTCGCCGGCACGGCGCTGAATGGGAGAGTCAAGTCCTCGGTTAGGGCTCGCATTGTGGATGTTACTCGCGTCATAAGCAATGCGTTGGCTCTGATTAACGAGTACGCTGAAAATCAAAAAAGCTAG

Protein sequence

MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEELKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISNALALINEYAENQKS
Homology
BLAST of Lag0019918 vs. NCBI nr
Match: XP_038882396.1 (21 kDa protein-like [Benincasa hispida])

HSP 1 Score: 332.8 bits (852), Expect = 2.0e-87
Identity = 173/195 (88.72%), Postives = 182/195 (93.33%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAK A LLLLLSI TIAAT T  AR GGASNFI+S CS  TYPDLC QSLSNFASTIQQN
Sbjct: 1   MAKPASLLLLLSILTIAATATATARNGGASNFIKSECSVATYPDLCVQSLSNFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL+VSLSRAQS RSFVWKLTKF+GLKPRE+AALKDCMEEV DTVDRL+KSVEE
Sbjct: 61  PRQLVQTALIVSLSRAQSTRSFVWKLTKFSGLKPREHAALKDCMEEVSDTVDRLNKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGR+KSSVRARIVDVTRV
Sbjct: 121 LKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRIKSSVRARIVDVTRV 180

Query: 181 ISNALALINEYAENQ 196
           ISNAL+LIN+YAENQ
Sbjct: 181 ISNALSLINKYAENQ 195

BLAST of Lag0019918 vs. NCBI nr
Match: XP_023551839.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 330.9 bits (847), Expect = 7.6e-87
Identity = 171/195 (87.69%), Postives = 185/195 (94.87%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKSA LLLLLSI TIAA TT+AAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQN
Sbjct: 1   MAKSASLLLLLSILTIAAATTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK REYAAL+DCMEEVGDTVDRLSKSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALALINEYAENQ 196
           ISNAL+LIN+YAEN+
Sbjct: 181 ISNALSLINKYAENE 195

BLAST of Lag0019918 vs. NCBI nr
Match: XP_022922994.1 (21 kDa protein-like [Cucurbita moschata] >XP_022985513.1 21 kDa protein-like [Cucurbita maxima] >KAG7014820.1 hypothetical protein SDJN02_22449, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 326.6 bits (836), Expect = 1.4e-85
Identity = 171/195 (87.69%), Postives = 185/195 (94.87%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKSA LLLLLSI TIAA TT+AAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQN
Sbjct: 1   MAKSASLLLLLSILTIAA-TTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK REYAAL+DCMEEVGDTVDRLSKSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALALINEYAENQ 196
           ISNAL+LIN+YAEN+
Sbjct: 181 ISNALSLINKYAENE 194

BLAST of Lag0019918 vs. NCBI nr
Match: XP_008439100.1 (PREDICTED: 21 kDa protein-like [Cucumis melo] >KAA0067679.1 21 kDa protein-like [Cucumis melo var. makuwa] >TYK23683.1 21 kDa protein-like [Cucumis melo var. makuwa])

HSP 1 Score: 310.5 bits (794), Expect = 1.1e-80
Identity = 166/196 (84.69%), Postives = 180/196 (91.84%), Query Frame = 0

Query: 1   MAKS-AFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQ 60
           MAKS + LLLLLSI TI+A    +A  GGASNFI+S+CSA TYPDLC QSLS+FASTIQ+
Sbjct: 1   MAKSLSLLLLLLSILTISA----SAFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQR 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVE 120
           NPRQLVQTAL VSLSRAQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRL+KSVE
Sbjct: 61  NPRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVE 120

Query: 121 ELKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTR 180
           ELKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAG+ALNGR+KSSVR RIVDVTR
Sbjct: 121 ELKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTR 180

Query: 181 VISNALALINEYAENQ 196
           VISNAL+LIN+YAE Q
Sbjct: 181 VISNALSLINKYAETQ 192

BLAST of Lag0019918 vs. NCBI nr
Match: XP_022140842.1 (21 kDa protein-like [Momordica charantia])

HSP 1 Score: 309.7 bits (792), Expect = 1.8e-80
Identity = 162/195 (83.08%), Postives = 180/195 (92.31%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MA+SAFLL+LLSI ++AA T+ AA   GASNFI+S+CSA TYPDLC QSLSNFASTIQQN
Sbjct: 1   MARSAFLLVLLSILSVAA-TSGAAGNAGASNFIKSKCSAATYPDLCVQSLSNFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLSRAQSARS VWKLTKF GL+PRE+AALKDC+EEV DTVDRLSKSVEE
Sbjct: 61  PRQLVQTALFVSLSRAQSARSHVWKLTKFEGLRPREHAALKDCLEEVSDTVDRLSKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRV G+ +KDFLWHISNVETWVSAAMTDENTCSDGF+G A+NGR+KSSVRARIVDV+RV
Sbjct: 121 LKRVHGAHEKDFLWHISNVETWVSAAMTDENTCSDGFSGKAMNGRIKSSVRARIVDVSRV 180

Query: 181 ISNALALINEYAENQ 196
           ISNALALI++YAENQ
Sbjct: 181 ISNALALISKYAENQ 194

BLAST of Lag0019918 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 198.0 bits (502), Expect = 1.0e-49
Identity = 98/184 (53.26%), Postives = 135/184 (73.37%), Query Frame = 0

Query: 13  IFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQNPRQLVQTALVVS 72
           +F+       AA    A+ FI++ C+ TTYP +C QSLS +A TIQ NP++L  TAL VS
Sbjct: 9   VFSALLVILAAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVS 68

Query: 73  LSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEELKRVSGSKQKDF 132
           L+R Q A++F+ +L KF GLK R+YAA+ DC+EEV D++DR+S+S +E+K +S +K  DF
Sbjct: 69  LTRTQQAQTFMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDF 128

Query: 133 LWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRVISNALALINEYA 192
            + +SNVETWVSAA+TDE TC DGFAG  ++G++K SVRA++V V RV SNALAL+N +A
Sbjct: 129 TFRMSNVETWVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNFA 188

Query: 193 ENQK 197
              K
Sbjct: 189 AKHK 192

BLAST of Lag0019918 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 187.2 bits (474), Expect = 1.8e-46
Identity = 100/200 (50.00%), Postives = 140/200 (70.00%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTT------AARKGGASNFIRSRCSATTYPDLCYQSLSNFA 60
           MAK  F  L L + + A  T +      A     A NFI++ C ATTYP +C  SL+ +A
Sbjct: 1   MAKQIFYTLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYA 60

Query: 61  STIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRL 120
           ++IQ +PR+L +TAL V++++AQS + FVW+L +FT LK RE  A+KDC+EE+ D VDRL
Sbjct: 61  NSIQTSPRRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRL 120

Query: 121 SKSVEELKRVSGSKQKDFLW-HISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRAR 180
           + S+ E+K    +K +D  W H+SN +TW SAA+T+ NTCSDGFAG  ++GRVK+SVRAR
Sbjct: 121 TMSIHEVKMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRAR 180

Query: 181 IVDVTRVISNALALINEYAE 194
           I+++ R  SNALALIN +A+
Sbjct: 181 ILNLGRGTSNALALINAFAK 200

BLAST of Lag0019918 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 175.3 bits (443), Expect = 7.0e-43
Identity = 92/198 (46.46%), Postives = 136/198 (68.69%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAAR-----KGGASNFIRSRCSATTYPDLCYQSLSNFAS 60
           MA++  L L+L +  ++      AR       G + FI++ C  T+YPD C+QSLS++AS
Sbjct: 1   MARNFELSLILFVLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYAS 60

Query: 61  TIQQNPRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLS 120
            I++ PR+L +TAL VS++RA+SA+++V ++T + G+  R++ A+ DC+EE+GDTVDRLS
Sbjct: 61  EIKKQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLS 120

Query: 121 KSVEELKRV-SGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARI 180
            S++ELK +  G   +DF + +SNV TW SAA+TDE  C DGF G A+ G +KS +R  I
Sbjct: 121 NSLKELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHI 180

Query: 181 VDVTRVISNALALINEYA 193
           V V    SNALALIN++A
Sbjct: 181 VSVAEETSNALALINDFA 198

BLAST of Lag0019918 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 169.9 bits (429), Expect = 3.0e-41
Identity = 96/198 (48.48%), Postives = 138/198 (69.70%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           +  + FL++LLSI TI  +++    +  +  FI S C  T YP LC  +LS +A+ I+ N
Sbjct: 3   LKNTIFLVILLSI-TILQSSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIRHN 62

Query: 61  PRQ-LVQTALVVSLSRAQSARSFVWKLTKFT-GLKPREYAALKDCMEEVGDTVDRLSKSV 120
             Q L QTAL++SL+RA+S   FV KLTK T   K REY A+KDC+E +G++VDRL++SV
Sbjct: 63  NDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQSV 122

Query: 121 EELKRVSGS-KQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDV 180
           +EL R   +   +DF+W +SNV+TWVSAA+TDE TC DGF+  A+ G+VK  +R ++V V
Sbjct: 123 KELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVVHV 182

Query: 181 TRVISNALALINEYAENQ 196
            +V SNALAL+N++AE +
Sbjct: 183 AQVTSNALALVNQFAEKR 199

BLAST of Lag0019918 vs. ExPASy Swiss-Prot
Match: Q84WE4 (Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 5.6e-32
Identity = 81/198 (40.91%), Postives = 119/198 (60.10%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTA-ARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQ 60
           MA +  L L+   F +  T +T   R  GA + + S C   +YP LC ++LS+++     
Sbjct: 1   MAPTQNLFLVAIAFAVIFTASTVHGRHNGAEDIVHSSCEHASYPSLCVRTLSSYSGPTIT 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVE 120
           N R L Q A+ +SLS AQSA   +  +    G K +E AAL DC+E +GD+VD LS+++ 
Sbjct: 61  NRRDLAQAAIKISLSHAQSAAKKLAVVRDSVGKKKQEKAALVDCVEMIGDSVDELSRTLG 120

Query: 121 ELK--RVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDV 180
            LK  RVSG   K+F W +SN +TW SAA+TD++TC DGF G   +G +K+ V+  +  V
Sbjct: 121 VLKHLRVSGGSAKEFRWQMSNAQTWASAALTDDDTCLDGFQGMD-DGEIKTEVKQWMTKV 180

Query: 181 TRVISNALALINEYAENQ 196
            RV SNAL ++N+  E +
Sbjct: 181 ARVTSNALYMVNQLDETR 197

BLAST of Lag0019918 vs. ExPASy TrEMBL
Match: A0A6J1JDT9 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483500 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 7.0e-86
Identity = 171/195 (87.69%), Postives = 185/195 (94.87%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKSA LLLLLSI TIAA TT+AAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQN
Sbjct: 1   MAKSASLLLLLSILTIAA-TTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK REYAAL+DCMEEVGDTVDRLSKSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALALINEYAENQ 196
           ISNAL+LIN+YAEN+
Sbjct: 181 ISNALSLINKYAENE 194

BLAST of Lag0019918 vs. ExPASy TrEMBL
Match: A0A6J1E526 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430812 PE=4 SV=1)

HSP 1 Score: 326.6 bits (836), Expect = 7.0e-86
Identity = 171/195 (87.69%), Postives = 185/195 (94.87%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MAKSA LLLLLSI TIAA TT+AAR GGASNFI+S+CSA TYPDLC QSLS FASTIQQN
Sbjct: 1   MAKSASLLLLLSILTIAA-TTSAARNGGASNFIKSKCSAATYPDLCVQSLSTFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTALVVSLSRAQS RSFVWK+TKF+GLK REYAAL+DCMEEVGDTVDRLSKSVEE
Sbjct: 61  PRQLVQTALVVSLSRAQSTRSFVWKMTKFSGLKKREYAALRDCMEEVGDTVDRLSKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRVSGSKQKDF WH+SNVETWVSAAMTDENTCSDGFAG+ALNGR+KSS+RARIVDV+RV
Sbjct: 121 LKRVSGSKQKDFQWHMSNVETWVSAAMTDENTCSDGFAGSALNGRIKSSIRARIVDVSRV 180

Query: 181 ISNALALINEYAENQ 196
           ISNAL+LIN+YAEN+
Sbjct: 181 ISNALSLINKYAENE 194

BLAST of Lag0019918 vs. ExPASy TrEMBL
Match: A0A5A7VRA6 (21 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00490 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 5.2e-81
Identity = 166/196 (84.69%), Postives = 180/196 (91.84%), Query Frame = 0

Query: 1   MAKS-AFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQ 60
           MAKS + LLLLLSI TI+A    +A  GGASNFI+S+CSA TYPDLC QSLS+FASTIQ+
Sbjct: 1   MAKSLSLLLLLLSILTISA----SAFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQR 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVE 120
           NPRQLVQTAL VSLSRAQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRL+KSVE
Sbjct: 61  NPRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVE 120

Query: 121 ELKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTR 180
           ELKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAG+ALNGR+KSSVR RIVDVTR
Sbjct: 121 ELKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTR 180

Query: 181 VISNALALINEYAENQ 196
           VISNAL+LIN+YAE Q
Sbjct: 181 VISNALSLINKYAETQ 192

BLAST of Lag0019918 vs. ExPASy TrEMBL
Match: A0A1S3AXX1 (21 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483991 PE=4 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 5.2e-81
Identity = 166/196 (84.69%), Postives = 180/196 (91.84%), Query Frame = 0

Query: 1   MAKS-AFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQ 60
           MAKS + LLLLLSI TI+A    +A  GGASNFI+S+CSA TYPDLC QSLS+FASTIQ+
Sbjct: 1   MAKSLSLLLLLLSILTISA----SAFNGGASNFIKSKCSAATYPDLCVQSLSSFASTIQR 60

Query: 61  NPRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVE 120
           NPRQLVQTAL VSLSRAQS RSFVWKLTKF+GLKPRE AALKDCMEEVGDTVDRL+KSVE
Sbjct: 61  NPRQLVQTALAVSLSRAQSTRSFVWKLTKFSGLKPRERAALKDCMEEVGDTVDRLNKSVE 120

Query: 121 ELKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTR 180
           ELKRVSGSK+KDFLWHISNVETWVSAAMTDENTCSDGFAG+ALNGR+KSSVR RIVDVTR
Sbjct: 121 ELKRVSGSKKKDFLWHISNVETWVSAAMTDENTCSDGFAGSALNGRIKSSVRGRIVDVTR 180

Query: 181 VISNALALINEYAENQ 196
           VISNAL+LIN+YAE Q
Sbjct: 181 VISNALSLINKYAETQ 192

BLAST of Lag0019918 vs. ExPASy TrEMBL
Match: A0A6J1CI81 (21 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011412 PE=4 SV=1)

HSP 1 Score: 309.7 bits (792), Expect = 8.8e-81
Identity = 162/195 (83.08%), Postives = 180/195 (92.31%), Query Frame = 0

Query: 1   MAKSAFLLLLLSIFTIAATTTTAARKGGASNFIRSRCSATTYPDLCYQSLSNFASTIQQN 60
           MA+SAFLL+LLSI ++AA T+ AA   GASNFI+S+CSA TYPDLC QSLSNFASTIQQN
Sbjct: 1   MARSAFLLVLLSILSVAA-TSGAAGNAGASNFIKSKCSAATYPDLCVQSLSNFASTIQQN 60

Query: 61  PRQLVQTALVVSLSRAQSARSFVWKLTKFTGLKPREYAALKDCMEEVGDTVDRLSKSVEE 120
           PRQLVQTAL VSLSRAQSARS VWKLTKF GL+PRE+AALKDC+EEV DTVDRLSKSVEE
Sbjct: 61  PRQLVQTALFVSLSRAQSARSHVWKLTKFEGLRPREHAALKDCLEEVSDTVDRLSKSVEE 120

Query: 121 LKRVSGSKQKDFLWHISNVETWVSAAMTDENTCSDGFAGTALNGRVKSSVRARIVDVTRV 180
           LKRV G+ +KDFLWHISNVETWVSAAMTDENTCSDGF+G A+NGR+KSSVRARIVDV+RV
Sbjct: 121 LKRVHGAHEKDFLWHISNVETWVSAAMTDENTCSDGFSGKAMNGRIKSSVRARIVDVSRV 180

Query: 181 ISNALALINEYAENQ 196
           ISNALALI++YAENQ
Sbjct: 181 ISNALALISKYAENQ 194

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038882396.12.0e-8788.7221 kDa protein-like [Benincasa hispida][more]
XP_023551839.17.6e-8787.6921 kDa protein-like [Cucurbita pepo subsp. pepo][more]
XP_022922994.11.4e-8587.6921 kDa protein-like [Cucurbita moschata] >XP_022985513.1 21 kDa protein-like [Cu... [more]
XP_008439100.11.1e-8084.69PREDICTED: 21 kDa protein-like [Cucumis melo] >KAA0067679.1 21 kDa protein-like ... [more]
XP_022140842.11.8e-8083.0821 kDa protein-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
P174071.0e-4953.2621 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1[more]
Q9STY51.8e-4650.00Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1[more]
Q9SB377.0e-4346.46Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1[more]
Q9SI723.0e-4148.48Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1[more]
Q84WE45.6e-3240.91Pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PMEI3 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JDT97.0e-8687.6921 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483500 PE=4 SV=1[more]
A0A6J1E5267.0e-8687.6921 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430812 PE=4 SV=1[more]
A0A5A7VRA65.2e-8184.6921 kDa protein-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G... [more]
A0A1S3AXX15.2e-8184.6921 kDa protein-like OS=Cucumis melo OX=3656 GN=LOC103483991 PE=4 SV=1[more]
A0A6J1CI818.8e-8183.0821 kDa protein-like OS=Momordica charantia OX=3673 GN=LOC111011412 PE=4 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 104..124
NoneNo IPR availablePANTHERPTHR31080:SF87PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEINcoord: 8..194
NoneNo IPR availablePANTHERPTHR31080PECTINESTERASE INHIBITOR-LIKEcoord: 8..194
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 37..192
e-value: 2.55218E-50
score: 157.985
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 28..187
e-value: 1.8E-55
score: 200.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..187
e-value: 1.8E-38
score: 132.3
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 5..191
e-value: 5.5E-43
score: 144.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 29..191
e-value: 3.3E-43
score: 149.3
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..190

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0019918.1Lag0019918.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0009641 shade avoidance
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity