Lag0018798 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0018798
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	TIR domain-containing protein-like
Location	chr5: 34650869 .. 34653128 (-)
RNA-Seq Expression	Lag0018798
Synteny	Lag0018798

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGAACCGCGTGGCTGCTGTGGCGAAGATTACGCCGAGGCAGTTTCTGGCTCAGCTGGGGAACAAGCAAAGGGCCATTGCGAAGCCTTGCGATGTGTTCATAAATCATAGAGGCGTCGACACGAAGAAGACGGTGGCGGCGCTGCTCTACGACAGGCTTGTCCGGGTGAACCTGCGCCCCTTCCTGGATTACAGGAATTTGAAGCCTGGAGAGAAGCTCTTCGATGAAATTCATGGCGCCATTCGCCAGTGTAAAGTTGCTGTCGCTGTCTTTTCGCCTCGCTATTGTGAGTCCTACTTTTGCCTTCATGAGTTGGCTATGATGATCGAGTCCAACAAGAAGGTATGTTTATGATTTTTTTTTTTTTTTTTATTAATGGAATAGTATTTGCGATTTCACAAACTAAAATCGAGTAAAAATTTATCCTAAAAAATAGAAAGGTAATTTGTTATAATCTCATAGAATTCTGAAATTCATCTCTCATGAACGTGATTGTTATATGGATTATTCTCAAATCAAGGATGTGGTTACTTTATAAGAATTAAGATGTAAATAAGCAATTAGATAACAATTAATAATATAATTTGGTTTCTATTTTGTTTTGAATTATTCTTGAACACAGGTCATTCCCATATTTTGTGATGTAAAACCATCTCAACTTCGCATAGAGGATCACGATGAATATGAAATAGATGAGCTAAGGAGGTTCAATTGGGCTCTCCAAGAAGCTAAACAGTTTGACGGACTCAAATTTAATTCCTCAAAAGAGTAAGATAAGCCTAAATATAAAATTTCTCCATATATATATATATATATATATGTCAACTTTAAATTTTGATTTATATATTATAGCAAAGTATTTTTTCTCCTAATTAATTGTTTCTCTGTGTTTCTTAATTAGGACCTTATCAGAAGTTGTCACAAAGGCTTCAAATATTGTCATCAGGAGTTTGGTTGAGATGGGCAACATCTAACATTTTCTTATTCTTTTACACATCATTCAATATTCATTATCTCCCCTCTCTCTCTCTTTCTTCAACAAAAAGAAAATGTATCATAAGATCCTGTAATTCTTTGGTAATAATAATATACAAAAAAATAAAAAAAAAATTCATTCATTCTTAAACTTTCACACTTGTTTGGTTAGTTAACACACATCTTTAAATAATTTGTTATTATTACTATTTTTTTGATTTGATATTTGAACTTTGTATAAAATTTTGTTTTGGTCTCCATTGCTAGAGTAAATATTGGAAAACAAATTAACACATAAATACTTAGGGTTTAGGGTTGAGGGACAATTATACCACTAGAAGAAATTATTGAACCTTTTGAAACAGAAAGAAGAAAAACGTTCAACCTTAGAAATAAATAAATAAATAAATAACATCGCTAAAATTCAAAAATCGACACAAATAAGCTTTAGGGACGAAAAATAAAACTTGCAAAAAACGTCAATGAAAGATAGTCGGACAAAATTTTTAGTGTTTGAAATTTTCGTTGCAAACTGCGACGGAATTAAAAAAAAAAAAAAAAAAATCGTTGTTTGCCACAAAATCTCTTTTTCGTTGCACGAATGCATGCGATGAAAATAACATATTTCATCACAATTCTTGCAATGAAAAGAGCAATCTCATTGCAATTCTTGCAACAAAAAGAGCAATCACAAGTTGTGTGCATTAACCTATGCGTCGAAACTTAGTTTTCATCACAAAATTCCTTTTTGACATTGCAACGATTCTAAAAGTTCAATTGAATCTAAAATTTCAAATATTCATCCATTTATTTTCCTCGTATATATCTTGTATTTAAACCAAAAACACGTTCTAAGGGATAAACATTATGTACAAATATTCAAATAGTATAAAATAATACATAATAATATATTAATAAAAACAAGGATAACAACATTTATATTTACCATAGCTTTTATAAGGATATCAAGTTTGTTGCAGTTACAAATTATGTATAATAAACTTTTATAAAACATAAAGACATCACCTATCTCCAAAATGAGCAAAAAAGTCCCAGTACCTATCTTCAAAATGTCAGGTTATACTCTCGTGACTTGCTTGTTCAGGCCGAGGCTCAGTTGGAGGAGGTGTTGCAGGAGGAAGTGGTATACTGGAAACAGAGGTCCAGGGAGTTATGGCTTCGAGAAGGGGATCGCAATACTCGGTGGTTCCACTGTCGAGCCTCTTACCGCCAGAAGCTTAATCGCATTGGAGGATTGGAGGATGATCAGGGAGTGTGGTAA

mRNA sequence

ATGATGAACCGCGTGGCTGCTGTGGCGAAGATTACGCCGAGGCAGTTTCTGGCTCAGCTGGGGAACAAGCAAAGGGCCATTGCGAAGCCTTGCGATGTGTTCATAAATCATAGAGGCGTCGACACGAAGAAGACGGTGGCGGCGCTGCTCTACGACAGGCTTGTCCGGGTGAACCTGCGCCCCTTCCTGGATTACAGGAATTTGAAGCCTGGAGAGAAGCTCTTCGATGAAATTCATGGCGCCATTCGCCAGTGTAAAGTTGCTGTCGCTGTCTTTTCGCCTCGCTATTGTGAGTCCTACTTTTGCCTTCATGAGTTGGCTATGATGATCGAGTCCAACAAGAAGGTCATTCCCATATTTTGTGATGTAAAACCATCTCAACTTCGCATAGAGGATCACGATGAATATGAAATAGATGAGCTAAGGAGGTTCAATTGGGCTCTCCAAGAAGCTAAACAGTTTGACGGACTCAAATTTAATTCCTCAAAAGAGACCTTATCAGAAGTTGTCACAAAGGCTTCAAATATTGTCATCAGGAGTTTGGCCGAGGCTCAGTTGGAGGAGGTGTTGCAGGAGGAAGTGGTATACTGGAAACAGAGGTCCAGGGAGTTATGGCTTCGAGAAGGGGATCGCAATACTCGGTGGTTCCACTGTCGAGCCTCTTACCGCCAGAAGCTTAATCGCATTGGAGGATTGGAGGATGATCAGGGAGTGTGGTAA

Coding sequence (CDS)

ATGATGAACCGCGTGGCTGCTGTGGCGAAGATTACGCCGAGGCAGTTTCTGGCTCAGCTGGGGAACAAGCAAAGGGCCATTGCGAAGCCTTGCGATGTGTTCATAAATCATAGAGGCGTCGACACGAAGAAGACGGTGGCGGCGCTGCTCTACGACAGGCTTGTCCGGGTGAACCTGCGCCCCTTCCTGGATTACAGGAATTTGAAGCCTGGAGAGAAGCTCTTCGATGAAATTCATGGCGCCATTCGCCAGTGTAAAGTTGCTGTCGCTGTCTTTTCGCCTCGCTATTGTGAGTCCTACTTTTGCCTTCATGAGTTGGCTATGATGATCGAGTCCAACAAGAAGGTCATTCCCATATTTTGTGATGTAAAACCATCTCAACTTCGCATAGAGGATCACGATGAATATGAAATAGATGAGCTAAGGAGGTTCAATTGGGCTCTCCAAGAAGCTAAACAGTTTGACGGACTCAAATTTAATTCCTCAAAAGAGACCTTATCAGAAGTTGTCACAAAGGCTTCAAATATTGTCATCAGGAGTTTGGCCGAGGCTCAGTTGGAGGAGGTGTTGCAGGAGGAAGTGGTATACTGGAAACAGAGGTCCAGGGAGTTATGGCTTCGAGAAGGGGATCGCAATACTCGGTGGTTCCACTGTCGAGCCTCTTACCGCCAGAAGCTTAATCGCATTGGAGGATTGGAGGATGATCAGGGAGTGTGGTAA

Protein sequence

MMNRVAAVAKITPRQFLAQLGNKQRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPIFCDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIRSLAEAQLEEVLQEEVVYWKQRSRELWLREGDRNTRWFHCRASYRQKLNRIGGLEDDQGVW

Homology

[Close]

BLAST of Lag0018798 vs. NCBI nr
Match: KAG7027684.1 (Toll/interleukin-1 receptor-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 322.8 bits (826), Expect = 2.5e-84
Identity = 155/182 (85.16%), Postives = 169/182 (92.86%), Query Frame = 0


Query: 1   MMNRVAAVAKITPRQFLAQLGNKQRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNLR 60
           MMNR++AVA ITPRQFLA LGN    I KPCDVFINHRGVDTKKTVAALLYDRLVR+NLR
Sbjct: 1   MMNRMSAVANITPRQFLAHLGNNHMPIVKPCDVFINHRGVDTKKTVAALLYDRLVRMNLR 60

Query: 61  PFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPIF 120
           PFLDY+NLKPGEKLFDEIHGAIR CKV VAVFSPRYCESYFCLHELAM+IESNKK+IPIF
Sbjct: 61  PFLDYKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIPIF 120

Query: 121 CDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIRS 180
           CD+KPS+LRIED ++YEIDELRRFNWALQ+AKQF+GLKFNS KETL EVVTK SN+VIR+
Sbjct: 121 CDIKPSELRIEDSNDYEIDELRRFNWALQQAKQFEGLKFNSFKETLPEVVTKISNVVIRN 180

Query: 181 LA 183
           LA
Sbjct: 181 LA 182

[Close]

BLAST of Lag0018798 vs. NCBI nr
Match: KAG6577380.1 (TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 319.3 bits (817), Expect = 2.8e-83
Identity = 154/182 (84.62%), Postives = 168/182 (92.31%), Query Frame = 0


Query: 1   MMNRVAAVAKITPRQFLAQLGNKQRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNLR 60
           MMNR++AVA ITPRQFLA LGN    I KPCDVFINHRGVDTKKTVAALLYDRLVR+NLR
Sbjct: 1   MMNRMSAVANITPRQFLAHLGNNHMPIVKPCDVFINHRGVDTKKTVAALLYDRLVRMNLR 60

Query: 61  PFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPIF 120
           PFLDY+NLKPGEKLFDEIHGAIR CKV VAVFSPRYCESYFCLHELAM+IESNKK+IPIF
Sbjct: 61  PFLDYKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIPIF 120

Query: 121 CDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIRS 180
           CD+KPS+LRIED ++YEIDELRRFNWALQ+AKQF+ LKFNS KETL EVVTK SN+VIR+
Sbjct: 121 CDIKPSELRIEDSNDYEIDELRRFNWALQQAKQFECLKFNSFKETLPEVVTKISNVVIRN 180

Query: 181 LA 183
           LA
Sbjct: 181 LA 182

[Close]

BLAST of Lag0018798 vs. NCBI nr
Match: XP_022984910.1 (TIR domain-containing protein-like [Cucurbita maxima])

HSP 1 Score: 318.9 bits (816), Expect = 3.6e-83
Identity = 153/181 (84.53%), Postives = 167/181 (92.27%), Query Frame = 0


Query: 2   MNRVAAVAKITPRQFLAQLGNKQRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRP 61
           MNR++AVA ITPRQFLA LGN    I KPCDVFINHRGVDTKKTVAALLYDRLVR+NLRP
Sbjct: 1   MNRMSAVANITPRQFLAHLGNNHMPIVKPCDVFINHRGVDTKKTVAALLYDRLVRMNLRP 60

Query: 62  FLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPIFC 121
           FLDY+NLKPGEKLFDEIHGAIR CKV VAVFSPRYCESYFCLHELAM+IESNKK+IPIFC
Sbjct: 61  FLDYKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIPIFC 120

Query: 122 DVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIRSL 181
           D+KPS+LRIED ++YEIDE+RRFNWAL EAKQF+GLKFNS KETL EVVTK SN+VIR+L
Sbjct: 121 DIKPSELRIEDSNDYEIDEVRRFNWALHEAKQFEGLKFNSFKETLPEVVTKISNVVIRNL 180

Query: 182 A 183
           A
Sbjct: 181 A 181

[Close]

BLAST of Lag0018798 vs. NCBI nr
Match: XP_023002843.1 (TMV resistance protein N-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 313.5 bits (802), Expect = 1.5e-81
Identity = 153/184 (83.15%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 2   MNRVAAVAKITPRQFLAQLGNK--QRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNL 61
           MNRVAAVAKITPR FLAQLG+K  QR IAKPCDVFINHRGVDTKKTVAALLYDRL+R N 
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 62  RPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPI 121
           RPFLDYRNLKPGEKLFDE+HGAIRQCKV VAVFSPRYCESYFCLHELAM+IESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 122 FCDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIR 181
           FCDV PSQLRI++ D YE ++LRRFNWAL++A Q +G+KFNSSK+TLS++VT+ASNIVI 
Sbjct: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180

Query: 182 SLAE 184
           SL E
Sbjct: 181 SLVE 184

[Close]

BLAST of Lag0018798 vs. NCBI nr
Match: XP_022942870.1 (TMV resistance protein N-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 313.5 bits (802), Expect = 1.5e-81
Identity = 154/184 (83.70%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 2   MNRVAAVAKITPRQFLAQLGNK--QRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNL 61
           MNRVAAVAKITPR FLAQLG+K  QR IAKPCDVFINHRGVDTKKTVAALLYDRL+R N 
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 62  RPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPI 121
           RPFLDYRNLKPGEKLFDE+HGAIRQCKV VAVFSPRYCESYFCLHELAM+IESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 122 FCDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIR 181
           FCDV PSQL I++ D YE ++LRRFNWAL++AKQ +G+KFNSSK+TLS+VVT+ASNIVI 
Sbjct: 121 FCDVNPSQLGIKEEDGYETEDLRRFNWALEQAKQLEGIKFNSSKQTLSDVVTRASNIVIS 180

Query: 182 SLAE 184
           SL E
Sbjct: 181 SLVE 184

[Close]

BLAST of Lag0018798 vs. ExPASy Swiss-Prot
Match: I1GTC2 (TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 1.8e-37
Identity = 80/168 (47.62%), Postives = 119/168 (70.83%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           +VFINHRGVDTK+TVA LLYDRL +  LR FLD  +++PG++L + I  AIR+C VAVA+
Sbjct: 54  EVFINHRGVDTKRTVARLLYDRLAQAGLRGFLDNMSMRPGDRLEERIGSAIRECTVAVAI 113

Query: 92  FSPRYCESYFCLHELAMMIESNKKVIPIFCDVKPSQL----RIEDHDEYEIDELRRFNWA 151
           FSP YC+S +CL ELAM++ES K +IPIF D+KPS L     + D + Y   +L RF +A
Sbjct: 114 FSPSYCDSEYCLRELAMLVESRKAIIPIFYDIKPSDLLLPQAVADSEVYLPRDLERFKFA 173

Query: 152 LQEAKQFDGLKFNSSKETLSEVVTKASNIVIRSLAEAQLEEVLQEEVV 196
           L+EAK   G+ ++S+   ++E+V+ A++ V+ ++   ++E V + E +
Sbjct: 174 LREAKHTVGITYDSATGDMAELVSAAADAVMYNM--EKMETVQRRETM 219

[Close]

BLAST of Lag0018798 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 7.5e-15
Identity = 42/111 (37.84%), Postives = 67/111 (60.36%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           DVF++ RG DT+KT    L++ L+   +  F+D + LK G+ +  E+  AI + + AV V
Sbjct: 13  DVFLSFRGEDTRKTFVGHLFNALIEKGIHTFMDDKELKRGKSISSELMKAIGESRFAVVV 72

Query: 92  FSPRYCESYFCLHELAMMIESNKK----VIPIFCDVKPSQLRIEDHDEYEI 139
           FS  Y  S +CL EL  ++E ++K    V+P+F DV PS +R + + EY +
Sbjct: 73  FSKNYASSTWCLEELVKILEIHEKFELIVVPVFYDVDPSTVR-KQNGEYAV 122

[Close]

BLAST of Lag0018798 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.9e-13
Identity = 52/163 (31.90%), Postives = 87/163 (53.37%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           DVF++ RG DT+KT  + LY+ L    ++ F D + L+ G  +  E+  AI + + A+ V
Sbjct: 13  DVFLSFRGEDTRKTFTSHLYEVLNDKGIKTFQDDKRLEYGATIPGELCKAIEESQFAIVV 72

Query: 92  FSPRYCESYFCLHELAMMIESNKK----VIPIFCDVKPSQLR---------IEDHD---E 151
           FS  Y  S +CL+EL  ++E   +    VIPIF DV PS +R          E+H+   +
Sbjct: 73  FSENYATSRWCLNELVKIMECKTRFKQTVIPIFYDVDPSHVRNQKESFAKAFEEHETKYK 132

Query: 152 YEIDELRRFNWALQEAKQFDGLKFNSSK---ETLSEVVTKASN 176
            +++ ++R+  AL EA    G   N  K   + + ++V + S+
Sbjct: 133 DDVEGIQRWRIALNEAANLKGSCDNRDKTDADCIRQIVDQISS 175

[Close]

BLAST of Lag0018798 vs. ExPASy Swiss-Prot
Match: Q9SSN3 (Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR PE=1 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 1.0e-11
Identity = 38/102 (37.25%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           DVF++ RG DT+    + LY  LVR ++R F D + L+ G++   E+   I   + AV V
Sbjct: 10  DVFLSFRGHDTRHNFISFLYKELVRRSIRTFKDDKELENGQRFSPELKSPIEVSRFAVVV 69

Query: 92  FSPRYCESYFCLHELAMMIESNKK----VIPIFCDVKPSQLR 130
            S  Y  S +CL EL  +++  KK    V+PIF  V+P+ +R
Sbjct: 70  VSENYAASSWCLDELVTIMDFEKKGSITVMPIFYGVEPNHVR 111

[Close]

BLAST of Lag0018798 vs. ExPASy Swiss-Prot
Match: F4JNB7 (Disease resistance protein RPP5 OS=Arabidopsis thaliana OX=3702 GN=RPP5 PE=1 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.8e-09
Identity = 33/102 (32.35%), Postives = 58/102 (56.86%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           DVF +  GVD +KT  + L + L   ++  F+D+  ++    +  E+  AIR+ ++++ +
Sbjct: 13  DVFPSFSGVDVRKTFLSHLIEALDGKSINTFIDH-GIERSRTIAPELISAIREARISIVI 72

Query: 92  FSPRYCESYFCLHELA----MMIESNKKVIPIFCDVKPSQLR 130
           FS  Y  S +CL+EL        +  + VIP+F DV PS++R
Sbjct: 73  FSKNYASSTWCLNELVEIHKCFNDLGQMVIPVFYDVDPSEVR 113

[Close]

BLAST of Lag0018798 vs. ExPASy TrEMBL
Match: A0A6J1J9V9 (TIR domain-containing protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483045 PE=4 SV=1)

HSP 1 Score: 318.9 bits (816), Expect = 1.8e-83
Identity = 153/181 (84.53%), Postives = 167/181 (92.27%), Query Frame = 0


Query: 2   MNRVAAVAKITPRQFLAQLGNKQRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRP 61
           MNR++AVA ITPRQFLA LGN    I KPCDVFINHRGVDTKKTVAALLYDRLVR+NLRP
Sbjct: 1   MNRMSAVANITPRQFLAHLGNNHMPIVKPCDVFINHRGVDTKKTVAALLYDRLVRMNLRP 60

Query: 62  FLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPIFC 121
           FLDY+NLKPGEKLFDEIHGAIR CKV VAVFSPRYCESYFCLHELAM+IESNKK+IPIFC
Sbjct: 61  FLDYKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIPIFC 120

Query: 122 DVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIRSL 181
           D+KPS+LRIED ++YEIDE+RRFNWAL EAKQF+GLKFNS KETL EVVTK SN+VIR+L
Sbjct: 121 DIKPSELRIEDSNDYEIDEVRRFNWALHEAKQFEGLKFNSFKETLPEVVTKISNVVIRNL 180

Query: 182 A 183
           A
Sbjct: 181 A 181

[Close]

BLAST of Lag0018798 vs. ExPASy TrEMBL
Match: A0A6J1KUS0 (TMV resistance protein N-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496557 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 7.4e-82
Identity = 153/184 (83.15%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 2   MNRVAAVAKITPRQFLAQLGNK--QRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNL 61
           MNRVAAVAKITPR FLAQLG+K  QR IAKPCDVFINHRGVDTKKTVAALLYDRL+R N 
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 62  RPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPI 121
           RPFLDYRNLKPGEKLFDE+HGAIRQCKV VAVFSPRYCESYFCLHELAM+IESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 122 FCDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIR 181
           FCDV PSQLRI++ D YE ++LRRFNWAL++A Q +G+KFNSSK+TLS++VT+ASNIVI 
Sbjct: 121 FCDVNPSQLRIKEDDGYETEDLRRFNWALEQANQLEGIKFNSSKQTLSDIVTRASNIVIS 180

Query: 182 SLAE 184
           SL E
Sbjct: 181 SLVE 184

[Close]

BLAST of Lag0018798 vs. ExPASy TrEMBL
Match: A0A6J1FX23 (TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447771 PE=4 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 7.4e-82
Identity = 154/184 (83.70%), Postives = 169/184 (91.85%), Query Frame = 0


Query: 2   MNRVAAVAKITPRQFLAQLGNK--QRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNL 61
           MNRVAAVAKITPR FLAQLG+K  QR IAKPCDVFINHRGVDTKKTVAALLYDRL+R N 
Sbjct: 1   MNRVAAVAKITPRLFLAQLGSKQLQRGIAKPCDVFINHRGVDTKKTVAALLYDRLIRGNF 60

Query: 62  RPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPI 121
           RPFLDYRNLKPGEKLFDE+HGAIRQCKV VAVFSPRYCESYFCLHELAM+IESNKKVIPI
Sbjct: 61  RPFLDYRNLKPGEKLFDELHGAIRQCKVGVAVFSPRYCESYFCLHELAMIIESNKKVIPI 120

Query: 122 FCDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIR 181
           FCDV PSQL I++ D YE ++LRRFNWAL++AKQ +G+KFNSSK+TLS+VVT+ASNIVI 
Sbjct: 121 FCDVNPSQLGIKEEDGYETEDLRRFNWALEQAKQLEGIKFNSSKQTLSDVVTRASNIVIS 180

Query: 182 SLAE 184
           SL E
Sbjct: 181 SLVE 184

[Close]

BLAST of Lag0018798 vs. ExPASy TrEMBL
Match: A0A6J1ERQ8 (TIR domain-containing protein-like OS=Cucurbita moschata OX=3662 GN=LOC111435930 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.7e-81
Identity = 150/178 (84.27%), Postives = 164/178 (92.13%), Query Frame = 0


Query: 5   VAAVAKITPRQFLAQLGNKQRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLD 64
           ++AVA ITPRQFLA LGN    I KPCDVFINHRGVDTKKTVAALLYDRLVR+NLRPFLD
Sbjct: 1   MSAVANITPRQFLAHLGNNHMPIVKPCDVFINHRGVDTKKTVAALLYDRLVRMNLRPFLD 60

Query: 65  YRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPIFCDVK 124
           Y+NLKPGEKLFDEIHGAIR CKV VAVFSPRYCESYFCLHELAM+IESNKK+IPIFCD+K
Sbjct: 61  YKNLKPGEKLFDEIHGAIRHCKVGVAVFSPRYCESYFCLHELAMIIESNKKLIPIFCDIK 120

Query: 125 PSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIRSLA 183
           PS+LRIED ++YEIDELRRFNWALQ+AKQF+ LKFNS KETL EVVTK SN+VIR+LA
Sbjct: 121 PSELRIEDDNDYEIDELRRFNWALQQAKQFECLKFNSFKETLPEVVTKISNVVIRNLA 178

[Close]

BLAST of Lag0018798 vs. ExPASy TrEMBL
Match: A0A6J1CP46 (disease resistance protein LAZ5 OS=Momordica charantia OX=3673 GN=LOC111012886 PE=4 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 2.0e-79
Identity = 158/186 (84.95%), Postives = 165/186 (88.71%), Query Frame = 0


Query: 2   MNRV-AAVAKITPRQFLAQLGNKQRA---IAKPCDVFINHRGVDTKKTVAALLYDRLVRV 61
           MNRV  AV KITP QFLAQLG    A   I KPCDVFINHRGVDTKKT+AALLYDRL+R 
Sbjct: 1   MNRVGGAVQKITPSQFLAQLGKMSLADQRIIKPCDVFINHRGVDTKKTLAALLYDRLLRF 60

Query: 62  NLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVI 121
           NLRPFLDYRNLKPGEKLFDEIHGAI QCKV VAVFSPRYCESYFCLHELAM++ES KKVI
Sbjct: 61  NLRPFLDYRNLKPGEKLFDEIHGAIWQCKVGVAVFSPRYCESYFCLHELAMIMESKKKVI 120

Query: 122 PIFCDVKPSQLRIEDHDEYEIDELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIV 181
           PIFCDVKPSQLRIED +EY  DELRRFNWAL+EAKQF GLKF+SSKETLSEVVTKASNIV
Sbjct: 121 PIFCDVKPSQLRIEDTNEYASDELRRFNWALEEAKQFVGLKFDSSKETLSEVVTKASNIV 180

Query: 182 IRSLAE 184
           IRSL E
Sbjct: 181 IRSLDE 186

[Close]

BLAST of Lag0018798 vs. TAIR 10
Match: AT1G52900.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 193.7 bits (491), Expect = 1.6e-49
Identity = 95/158 (60.13%), Postives = 119/158 (75.32%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           DVFINHRG DTK+ +A LLYD L   NLRPFLD +N+KPG+KLFD I+ AI   KVAV V
Sbjct: 39  DVFINHRGSDTKRNIATLLYDNLKSRNLRPFLDSKNMKPGDKLFDHINNAILTSKVAVTV 98

Query: 92  FSPRYCESYFCLHELAMMIESNKKVIPIFCDVKPSQLRIE-DHDEYEIDELRRFNWALQE 151
           FSP YC+SYFCLHELA+++ES K+VIPIFCD+KPSQL +  +      DE++RF WALQE
Sbjct: 99  FSPNYCDSYFCLHELALIMESRKRVIPIFCDIKPSQLDVMIERVTCSDDEIQRFRWALQE 158

Query: 152 AKQFDGLKFNSSKETLSEVVTKASNIVIRSLAEAQLEE 189
           AK   GL F+S K  LSEVVT AS++++  L E + ++
Sbjct: 159 AKDIVGLTFDSYKGNLSEVVTVASDVIVERLVELEAKD 196

[Close]

BLAST of Lag0018798 vs. TAIR 10
Match: AT1G61105.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 182.2 bits (461), Expect = 5.0e-46
Identity = 87/166 (52.41%), Postives = 117/166 (70.48%), Query Frame = 0


Query: 20  LGNKQRAIAKPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIH 79
           + N       PCD+FINHRG+DTKKT++ LLYD   R  L  FLD ++LKPG++LF EI 
Sbjct: 18  IANPTLPYPNPCDIFINHRGIDTKKTISGLLYDCFTRQRLTAFLDSKSLKPGDRLFVEID 77

Query: 80  GAIRQCKVAVAVFSPRYCESYFCLHELAMMIESNKKVIPIFCDVKPSQLRIEDHDEYEID 139
            AI+ C V +AVFSPRYC+SYFCLHELA+++++ K+VIPIFCDVKPS+L ++D       
Sbjct: 78  MAIKACGVGIAVFSPRYCDSYFCLHELALLVKNKKRVIPIFCDVKPSELCVKDDKTRPAA 137

Query: 140 ELRRFNWALQEAKQFDGLKFNSSKETLSEVVTKASNIVIRSLAEAQ 186
           E+RRF  AL+EAK   GL F++S    SE +  AS+ V ++L E +
Sbjct: 138 EIRRFQLALEEAKYTVGLTFDTSNGDWSEFLAMASDAVTKNLLEVE 183

[Close]

BLAST of Lag0018798 vs. TAIR 10
Match: AT1G17615.1 (Disease resistance protein (TIR-NBS class) )

HSP 1 Score: 79.3 bits (194), Expect = 4.5e-15
Identity = 38/102 (37.25%), Postives = 62/102 (60.78%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           DVF++ RG DT++T  + LY  L+ + +R F D   L+ G ++  ++  AI   K+AV +
Sbjct: 16  DVFLSFRGKDTRRTFISFLYKELIEMGIRTFKDDVELQSGRRIASDLLAAIENSKIAVVI 75

Query: 92  FSPRYCESYFCLHELAMMIESNKK----VIPIFCDVKPSQLR 130
            S  Y  S +CL EL M+++  KK    V+PIF +V+P+ +R
Sbjct: 76  ISKNYSASPWCLQELVMIMDVEKKGSIIVMPIFYNVEPAHVR 117

[Close]

BLAST of Lag0018798 vs. TAIR 10
Match: AT4G09430.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 77.0 bits (188), Expect = 2.3e-14
Identity = 48/162 (29.63%), Postives = 87/162 (53.70%), Query Frame = 0


Query: 29  KPCDVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVA 88
           K  DVF++ RG DT+  + + L+  LV V +R F D + L+ G+ + +++  AI+    A
Sbjct: 13  KEYDVFLSFRGADTRNNIVSYLHKALVDVGIRTFKDDKELEEGDIISEKLVNAIQTSWFA 72

Query: 89  VAVFSPRYCESYFCLHEL----AMMIESNKKVIPIFCDVKPSQLRIEDHDEYEI------ 148
           V V S +Y  S +CL EL     + I+ +  V+PIF  V+PS +R +  + +E+      
Sbjct: 73  VVVLSEKYVTSSWCLEELRHIMELSIQDDIIVVPIFYKVEPSDVRYQ-KNSFEVKLQHYR 132

Query: 149 --DELRRFNWALQEAKQFDGLKFNSSKE---TLSEVVTKASN 176
             +++ ++  AL +     G  F +  +    ++E+V+K SN
Sbjct: 133 DPEKILKWKGALTQVGNMSGKHFQTCSDEATNIAEIVSKISN 173

[Close]

BLAST of Lag0018798 vs. TAIR 10
Match: AT1G72920.1 (Toll-Interleukin-Resistance (TIR) domain family protein )

HSP 1 Score: 76.6 bits (187), Expect = 2.9e-14
Identity = 51/167 (30.54%), Postives = 85/167 (50.90%), Query Frame = 0


Query: 32  DVFINHRGVDTKKTVAALLYDRLVRVNLRPFLDYRNLKPGEKLFDEIHGAIRQCKVAVAV 91
           DVF++ RG+DT++   + LY  LVR  +R F D + L+ G ++  E+  AI + K AV V
Sbjct: 10  DVFLSFRGLDTRRNFISFLYKELVRRKIRTFKDDKELENGRRISPELKRAIEESKFAVVV 69

Query: 92  FSPRYCESYFCLHELAMMIESNKK----VIPIFCDVKPSQLRIEDHDEYEIDELRRFNWA 151
            S  Y  S +CL EL  +++   K    V+PIF  V P  LR +  D  E        + 
Sbjct: 70  VSVNYAASPWCLDELVKIMDFENKGSITVMPIFYGVDPCHLRRQIGDVAE-------QFK 129

Query: 152 LQEAKQFDGLKFNSSKETLSEVVTKASNIVIRSLAEAQLEEVLQEEV 195
             EA++ D  K  S +  L+ + + +    ++   EA L + + +++
Sbjct: 130 KHEAREEDHEKVASWRRALTSLASISGECSLKWEDEANLVDEIADKI 169

The following BLAST results are available for this feature:

BLAST of Lag0018798 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027684.1	2.5e-84	85.16	Toll/interleukin-1 receptor-like protein, partial [Cucurbita argyrosperma subsp....	[more]
KAG6577380.1	2.8e-83	84.62	TIR-only protein, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022984910.1	3.6e-83	84.53	TIR domain-containing protein-like [Cucurbita maxima]	[more]
XP_023002843.1	1.5e-81	83.15	TMV resistance protein N-like isoform X1 [Cucurbita maxima]	[more]
XP_022942870.1	1.5e-81	83.70	TMV resistance protein N-like isoform X1 [Cucurbita moschata]	[more]

BLAST of Lag0018798 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
I1GTC2	1.8e-37	47.62	TIR-only protein OS=Brachypodium distachyon OX=15368 GN=BRADI_1g24377v3 PE=1 SV=...	[more]
A0A290U7C4	7.5e-15	37.84	Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV...	[more]
Q40392	1.9e-13	31.90	TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1	[more]
Q9SSN3	1.0e-11	37.25	Toll/interleukin-1 receptor-like protein OS=Arabidopsis thaliana OX=3702 GN=TIR ...	[more]
F4JNB7	2.8e-09	32.35	Disease resistance protein RPP5 OS=Arabidopsis thaliana OX=3702 GN=RPP5 PE=1 SV=...	[more]

BLAST of Lag0018798 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J9V9	1.8e-83	84.53	TIR domain-containing protein-like OS=Cucurbita maxima OX=3661 GN=LOC111483045 P...	[more]
A0A6J1KUS0	7.4e-82	83.15	TMV resistance protein N-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11149...	[more]
A0A6J1FX23	7.4e-82	83.70	TMV resistance protein N-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1ERQ8	3.7e-81	84.27	TIR domain-containing protein-like OS=Cucurbita moschata OX=3662 GN=LOC111435930...	[more]
A0A6J1CP46	2.0e-79	84.95	disease resistance protein LAZ5 OS=Momordica charantia OX=3673 GN=LOC111012886 P...	[more]

BLAST of Lag0018798 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G52900.1	1.6e-49	60.13	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G61105.1	5.0e-46	52.41	Toll-Interleukin-Resistance (TIR) domain family protein	[more]
AT1G17615.1	4.5e-15	37.25	Disease resistance protein (TIR-NBS class)	[more]
AT4G09430.1	2.3e-14	29.63	Disease resistance protein (TIR-NBS-LRR class) family	[more]
AT1G72920.1	2.9e-14	30.54	Toll-Interleukin-Resistance (TIR) domain family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	SMART	SM00255	till_3	coord: 30..176 e-value: 5.4E-18 score: 75.8
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PFAM	PF01582	TIR	coord: 32..129 e-value: 1.9E-19 score: 70.1
IPR000157	Toll/interleukin-1 receptor homology (TIR) domain	PROSITE	PS50104	TIR	coord: 29..177 score: 27.916536
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	GENE3D	3.40.50.10140		coord: 27..183 e-value: 1.1E-33 score: 118.3
IPR035897	Toll/interleukin-1 receptor homology (TIR) domain superfamily	SUPERFAMILY	52200	Toll/Interleukin receptor TIR domain	coord: 29..143
None	No IPR available	PANTHER	PTHR31008	COP1-INTERACTING PROTEIN-RELATED	coord: 27..192
None	No IPR available	PANTHER	PTHR31008:SF16	TOLL-INTERLEUKIN-RESISTANCE (TIR) DOMAIN FAMILY PROTEIN	coord: 27..192

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0018798.1	Lag0018798.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0007165	signal transduction
molecular_function	GO:0003953	NAD+ nucleosidase activity