Lag0018532 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0018532
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr5: 29048293 .. 29049657 (+)
RNA-Seq ExpressionLag0018532
SyntenyLag0018532
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTAGATCAAGACCCCTGGATTAATTTCAAAAGTGCTAGAATCCCCATCAAAGTGAAGGATGATTTTAAGAACAAGAGAGTTAACGTGCTGATTGAGGAAGATGGAAGCTGGAAAGAGTCTTTAATCAAAGAGGTGTTCACATCCATGGAAGCTGAAGAAATCATAAATATGCCAAGATGCAAGTCCAAAGCTAAGGATGAAATTATATGGAATGAGGATCCTAAAGGTCAATTCACGGTGAAGAGCGCTTACCATCTAGCTACTAATTTAGAAGCTAGAAATGAAGCTTCTTCATCTGATAATCAAGCTTCAACAACCATTTGGAACTCGATCTGGAAGATTAAATGCATTCCGAGAGCAAAAATTGCAGCTTGGAAGATTATCAATGACATTATTCCTTCTAATTACAATGTTAAAAGGAAAGGAATTAATTCTAATGATCGATGTGTTTTTTACAGGGAGCATGAGGAAACTACAGAACATTTGATATGGATGTGTAAATTCTCTAAGAAGGTTTGGATTGATTTTCTCCCCAATTTACACAACTTCTTTGCTAATTGCAGAAGCTACGGGAAAGCGTTGGATTTTTGGGAAGGGATGTGTAGATGCTTAAAAAGAGAAGACACCAAAGTCGCAGTGCTTATTATGTGGAGCTTATGGAATACGAGAAACATATGCTTTCACAACAATCAGAGCCCAAATTTTCAGAGCACCAGAAGATCAATTCAAAACTTTATAGAAGAAGAAGAAGAAAGGTTGATCGAGCACCTGATTCCGATGGAAGACCCGTCTCCGACAGCGATGGAGAACCAGCTGAGTCACCCCCATTGGTCAGTGCCAGCGCCAAATCGCTGGAAACTCAACGTGGATGCCTCCTGGGATGAGGAATCAGAGAAATGAGGGGTGGGGTGGTTAATTTGTGACTCAGTTGGGTCTCTAATTGGAGTGGGTTGCAAGCAGATTGCATGGAGATGGCCAATTAAAGTTCTAGAAGCAAAAGCTATTTCTGAGGGTTTGAAGACTTACATTAAAGCGACGGAGATGGAAGATCTCGAGATCAGGCCAGAGATGGAAGTGGAGGCGGATTCTCTTGAGGTGATTCAAACCATCAACAAGAAGTCTGTGGACCTTTCGGAGTTGTCGCTGGTGATAGACGAAATCGAAGAGATGGTTCCTGGTGCAAGAGTCGCCTCGTTTGTGAAATGCTCGAGATCGAGCAATGATATTGCACATAAGATTGCGCGCGCTGGAGTCATTCATGGAGATTTCTGTTATTTTTTTGGTCACCCCTCCAATTCTGTTTTGAGGGAATTTCAGGGTGCAAGGATAAGAGTATCCCTGTGTGGCTTAAAAGATGTTTAG

mRNA sequence

ATGTTAGATCAAGACCCCTGGATTAATTTCAAAAGTGCTAGAATCCCCATCAAAGTGAAGGATGATTTTAAGAACAAGAGAGTTAACGTGCTGATTGAGGAAGATGGAAGCTGGAAAGAGTCTTTAATCAAAGAGGTGTTCACATCCATGGAAGCTGAAGAAATCATAAATATGCCAAGATGCAAGTCCAAAGCTAAGGATGAAATTATATGGAATGAGGATCCTAAAGGTCAATTCACGGTGAAGAGCGCTTACCATCTAGCTACTAATTTAGAAGCTAGAAATGAAGCTTCTTCATCTGATAATCAAGCTTCAACAACCATTTGGAACTCGATCTGGAAGATTAAATGCATTCCGAGAGCAAAAATTGCAGCTTGGAAGATTATCAATGACATTATTCCTTCTAATTACAATGTTAAAAGGAAAGGAATTAATTCTAATGATCGATGTGTTTTTTACAGGGAGCATGAGGAAACTACAGAACATTTGATATGGATGTGTAAATTCTCTAAGAAGATGCTTAAAAAGAGAAGACACCAAAGTCGCAGTGCTTATTATGTGGAGCTTATGGAATACGAGAAACATATGCTTTCACAACAATCAGAGCCCAAATTTTCAGAGCACCAGAAGATCAATTCAAAACTTTATAGAAGAAGAAGAAGAAAGGTTGATCGAGCACCTGATTCCGATGGAAGACCCGTCTCCGACAGCGATGGAGAACCAGCTGAGTCACCCCCATTGATTGCATGGAGATGGCCAATTAAAGTTCTAGAAGCAAAAGCTATTTCTGAGGGTTTGAAGACTTACATTAAAGCGACGGAGATGGAAGATCTCGAGATCAGGCCAGAGATGGAAGTGGAGGCGGATTCTCTTGAGGTGATTCAAACCATCAACAAGAAGTCTGTGGACCTTTCGGAGTTGTCGCTGGTGATAGACGAAATCGAAGAGATGGTTCCTGGTGCAAGAGTCGCCTCGTTTGTGAAATGCTCGAGATCGAGCAATGATATTGCACATAAGATTGCGCGCGCTGGAGTCATTCATGGAGATTTCTGTTATTTTTTTGGTCACCCCTCCAATTCTGTTTTGAGGGAATTTCAGGGTGCAAGGATAAGAGTATCCCTGTGTGGCTTAAAAGATGTTTAG

Coding sequence (CDS)

ATGTTAGATCAAGACCCCTGGATTAATTTCAAAAGTGCTAGAATCCCCATCAAAGTGAAGGATGATTTTAAGAACAAGAGAGTTAACGTGCTGATTGAGGAAGATGGAAGCTGGAAAGAGTCTTTAATCAAAGAGGTGTTCACATCCATGGAAGCTGAAGAAATCATAAATATGCCAAGATGCAAGTCCAAAGCTAAGGATGAAATTATATGGAATGAGGATCCTAAAGGTCAATTCACGGTGAAGAGCGCTTACCATCTAGCTACTAATTTAGAAGCTAGAAATGAAGCTTCTTCATCTGATAATCAAGCTTCAACAACCATTTGGAACTCGATCTGGAAGATTAAATGCATTCCGAGAGCAAAAATTGCAGCTTGGAAGATTATCAATGACATTATTCCTTCTAATTACAATGTTAAAAGGAAAGGAATTAATTCTAATGATCGATGTGTTTTTTACAGGGAGCATGAGGAAACTACAGAACATTTGATATGGATGTGTAAATTCTCTAAGAAGATGCTTAAAAAGAGAAGACACCAAAGTCGCAGTGCTTATTATGTGGAGCTTATGGAATACGAGAAACATATGCTTTCACAACAATCAGAGCCCAAATTTTCAGAGCACCAGAAGATCAATTCAAAACTTTATAGAAGAAGAAGAAGAAAGGTTGATCGAGCACCTGATTCCGATGGAAGACCCGTCTCCGACAGCGATGGAGAACCAGCTGAGTCACCCCCATTGATTGCATGGAGATGGCCAATTAAAGTTCTAGAAGCAAAAGCTATTTCTGAGGGTTTGAAGACTTACATTAAAGCGACGGAGATGGAAGATCTCGAGATCAGGCCAGAGATGGAAGTGGAGGCGGATTCTCTTGAGGTGATTCAAACCATCAACAAGAAGTCTGTGGACCTTTCGGAGTTGTCGCTGGTGATAGACGAAATCGAAGAGATGGTTCCTGGTGCAAGAGTCGCCTCGTTTGTGAAATGCTCGAGATCGAGCAATGATATTGCACATAAGATTGCGCGCGCTGGAGTCATTCATGGAGATTTCTGTTATTTTTTTGGTCACCCCTCCAATTCTGTTTTGAGGGAATTTCAGGGTGCAAGGATAAGAGTATCCCTGTGTGGCTTAAAAGATGTTTAG

Protein sequence

MLDQDPWINFKSARIPIKVKDDFKNKRVNVLIEEDGSWKESLIKEVFTSMEAEEIINMPRCKSKAKDEIIWNEDPKGQFTVKSAYHLATNLEARNEASSSDNQASTTIWNSIWKIKCIPRAKIAAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSKKMLKKRRHQSRSAYYVELMEYEKHMLSQQSEPKFSEHQKINSKLYRRRRRKVDRAPDSDGRPVSDSDGEPAESPPLIAWRWPIKVLEAKAISEGLKTYIKATEMEDLEIRPEMEVEADSLEVIQTINKKSVDLSELSLVIDEIEEMVPGARVASFVKCSRSSNDIAHKIARAGVIHGDFCYFFGHPSNSVLREFQGARIRVSLCGLKDV
Homology
BLAST of Lag0018532 vs. NCBI nr
Match: XP_040986468.1 (uncharacterized protein LOC121234565 [Juglans microcarpa x Juglans regia])

HSP 1 Score: 111.7 bits (278), Expect = 1.4e-20
Identity = 53/145 (36.55%), Postives = 84/145 (57.93%), Query Frame = 0

Query: 24  KNKRVNVLIE-EDGSWKESLIKEVFTSMEAEEIINMPRCKSKAKDEIIWNEDPKGQFTVK 83
           K+ +V  LI+ + G W E++I+ +F+  E E+I+ MP  + +AKD+I W    KG FTV+
Sbjct: 726 KDAKVEELIDRQKGEWNETMIRTIFSEEEVEQILCMPLSRGRAKDKIFWGPTKKGLFTVR 785

Query: 84  SAYHLATNLEARNEASSSDNQASTTIWNSIWKIKCIPRAKIAAWKIINDIIPSNYNVKRK 143
           SAY L   L+ RN+  SS  +    IW SIW ++     K+  WK  N ++P+  N+ R+
Sbjct: 786 SAYFLHMELKERNKGESSTEKKVGDIWKSIWDLEIPGVTKLFLWKAANSLLPTKVNLYRR 845

Query: 144 GINSNDRCVFYREHEETTEHLIWMC 168
            I+ + RC      EE+  H++W C
Sbjct: 846 NISMDKRCPMCDAEEESLIHILWEC 870

BLAST of Lag0018532 vs. NCBI nr
Match: XP_023875263.1 (uncharacterized protein LOC111987752 [Quercus suber])

HSP 1 Score: 108.6 bits (270), Expect = 1.2e-19
Identity = 57/173 (32.95%), Postives = 96/173 (55.49%), Query Frame = 0

Query: 4   QDPWINFKSARIPIKVK---DDFKNKRVNVLIEEDGS-WKESLIKEVFTSMEAEEIINMP 63
           +D W+   +   PI      DDF    V+ LI+ D   WK  L+K +F   EA+ I+++P
Sbjct: 44  EDKWLPTPTTYKPISPPCFFDDF--PMVSALIDLDTKRWKADLVKTIFLPFEADTILSIP 103

Query: 64  RCKSKAKDEIIWNEDPKGQFTVKSAYHLATNL-EARNEASSSDNQASTTIWNSIWKIKCI 123
            C S   D++IW  + KG FTV+SAY++A +L E  NE  SS   + + +W  +W +  +
Sbjct: 104 LCYSLPDDKLIWLGNTKGAFTVRSAYYVALSLVETSNEGESSFGDSQSLLWKKVWHLNLL 163

Query: 124 PRAKIAAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSK 172
            + +I AW+   + +P+  N++ +G+N++D C    +  E T H ++ C  SK
Sbjct: 164 EKIRIFAWRACMNALPTKQNLRVRGVNTDDSCPLCGQGPENTMHALFNCDCSK 214

BLAST of Lag0018532 vs. NCBI nr
Match: EEC72938.1 (hypothetical protein OsI_06800 [Oryza sativa Indica Group])

HSP 1 Score: 107.8 bits (268), Expect = 2.0e-19
Identity = 57/170 (33.53%), Postives = 95/170 (55.88%), Query Frame = 0

Query: 4   QDPWINFKSARIPIKVKDDFKNKRVNVLIEEDGSWKESLIKEVFTSMEAEEIINMPRCKS 63
           +DPWI    +R PI  K + + + V+ LI++  SW    I + F  M+AE I+N+ R  S
Sbjct: 343 RDPWIPRSFSRRPISAKRNCRLRWVSDLIDQSESWDTDKISQHFLPMDAEAILNI-RLSS 402

Query: 64  KAKDEII-WNEDPKGQFTVKSAYHLATNLEARNEASSSDNQASTTIWNSIWKIKCIPRAK 123
           + +D+ I W+ D  G+F+V+SAYHLA  L   +E SSS    ++  WN++WK     + K
Sbjct: 403 RLEDDFIAWHPDRLGRFSVRSAYHLAIALAQVDEGSSSSGNGNSRAWNALWKCNAPQKVK 462

Query: 124 IAAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSKK 173
           I AW+ I + +P+  N K++ +  +D C       E   H ++ C  +++
Sbjct: 463 IFAWRAITNSLPTLENKKKRKLEVSDICTICGVESEYVVHALFRCPHARQ 511

BLAST of Lag0018532 vs. NCBI nr
Match: EEC84753.1 (hypothetical protein OsI_31756 [Oryza sativa Indica Group])

HSP 1 Score: 107.5 bits (267), Expect = 2.7e-19
Identity = 53/170 (31.18%), Postives = 89/170 (52.35%), Query Frame = 0

Query: 4    QDPWINFKSARIPIKVKDDFKNKRVNVLIEEDGSWKESLIKEVFTSMEAEEIINMPRCKS 63
            +DPWI    +R PI  K ++K K V  L+ + G W  + ++++F  ++AE I+ +     
Sbjct: 1135 RDPWIPRDHSRRPITKKSNYKTKWVTDLLTDAGEWDVNRVRQIFWPIDAEVILKIRVPSQ 1194

Query: 64   KAKDEIIWNEDPKGQFTVKSAYHLATNLEARNEASSSDNQASTTIWNSIWKIKCIPRAKI 123
               D + W+ D  G+F+V+SAY LA +L + N  S+S     +  WN +WK     + KI
Sbjct: 1195 DVSDFVAWHPDRLGRFSVRSAYSLAVSLASENAFSASSAVDRSKAWNMLWKCNVPQKVKI 1254

Query: 124  AAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSKKM 174
             AWK+  D + +  N KR+ + + D C      EE + H +  C  +K +
Sbjct: 1255 FAWKVALDCLATMVNKKRRKLEATDVCAICGTEEEDSAHALCRCPHAKSL 1304

BLAST of Lag0018532 vs. NCBI nr
Match: ABA98898.1 (retrotransposon protein, putative, unclassified [Oryza sativa Japonica Group])

HSP 1 Score: 107.1 bits (266), Expect = 3.5e-19
Identity = 51/169 (30.18%), Postives = 91/169 (53.85%), Query Frame = 0

Query: 4   QDPWINFKSARIPIKVKDDFKNKRVNVLIEEDGSWKESLIKEVFTSMEAEEIINMPRCKS 63
           +DPW+    +R PI  K + + K V  L++++GSW   +I ++F  ++ E I ++     
Sbjct: 750 RDPWLPRDFSRRPITPKGNCRLKWVADLLDQNGSWDRDVISQLFWPLDGEIIKSIRLSSR 809

Query: 64  KAKDEIIWNEDPKGQFTVKSAYHLATNLEARNEASSSDNQASTTIWNSIWKIKCIPRAKI 123
           + +D I W  D  GQF+++SAY LA  L    E+SSS    +  +WN +WK     + K+
Sbjct: 810 QDEDFIAWYPDKMGQFSIRSAYMLAVQLANAGESSSSSGNDTKKVWNHVWKCNIPQKVKV 869

Query: 124 AAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSKK 173
            +W+ I++ +P+  N K++    +D C F     E   H +W C  +++
Sbjct: 870 FSWRAISNCLPTMVNKKKRNPELSDVCSFCGLEPEDVAHALWRCPHARR 918

BLAST of Lag0018532 vs. ExPASy TrEMBL
Match: A0A2N9H3I8 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34205 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 2.1e-22
Identity = 64/186 (34.41%), Postives = 105/186 (56.45%), Query Frame = 0

Query: 4   QDPWI-NFKSARI--PIKVKDDFKNKRVNVLIEEDG-SWKESLIKEVFTSMEAEEIINMP 63
           +D W+ +  S R+  P+ V D+F    V+ LI +D  +WK  L+ E+F   + + II +P
Sbjct: 487 KDQWLPSSTSFRVISPVNVLDEFAT--VDQLINQDSMTWKVQLLHEIFCPRDVDLIICIP 546

Query: 64  RCKSKAKDEIIWNEDPKGQFTVKSAYHLATNLEARNEASSSDNQASTTIWNSIWKIKCIP 123
               +  D +IW    +G F+VKSAYHL  +L    EA++S   +  +IW+SIW +K  P
Sbjct: 547 LSVRRPPDSLIWIVTKRGVFSVKSAYHLQLSLRNSQEAATSSRTSVHSIWSSIWAVKVPP 606

Query: 124 RAKIAAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSKKMLKKRRH 183
           + K+  WK  +DI+P+   +  KGI+ +  C++  E  ET +H++W C+F+ K+ K    
Sbjct: 607 KVKLFVWKACHDILPTRTKLFEKGISHSYSCLWCAEEPETCDHVLWGCEFAHKVWKSSSV 666

Query: 184 QSRSAY 186
              S Y
Sbjct: 667 AISSQY 670

BLAST of Lag0018532 vs. ExPASy TrEMBL
Match: A0A803PAX6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 1.1e-21
Identity = 64/179 (35.75%), Postives = 100/179 (55.87%), Query Frame = 0

Query: 2    LDQDPWINFKSA---RIPIKVKDDFKNKRVNVLIEEDGSWKESLIKEVFTSMEAEEIINM 61
            +++D W+   S    R P KV    K   ++ L EE+G WK  LIKE F + +   I+ M
Sbjct: 902  VNEDKWLPRPSTFTLRTPAKVP---KGTTMDTLKEENGEWKLDLIKESFHNDDIPIILGM 961

Query: 62   PRCKSKAKDEIIWNEDPKGQFTVKSAYHLATNLEARNEASSSDNQAST-TIWNSIWKIKC 121
              CK  + D++IW+    G +TV+S Y +A   E  N  +   ++A+T   W SIWKI+ 
Sbjct: 962  APCKKHSNDDLIWHFTSDGLYTVRSGYEVA---EIDNYKAGPSSEATTRKWWTSIWKIEA 1021

Query: 122  IPRAKIAAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSKKMLKK 177
             P+ +   W++ N  IP N  ++R+G+N N  C +  + EET EH +W C +SK++ KK
Sbjct: 1022 PPKIRNFLWRVCNGWIPVNTILQRRGMNINTSCYWCGQAEETIEHCLWFCPYSKQIWKK 1074

BLAST of Lag0018532 vs. ExPASy TrEMBL
Match: M5XK32 (Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=3760 GN=PRUPE_ppa016563mg PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 1.8e-21
Identity = 97/384 (25.26%), Postives = 175/384 (45.57%), Query Frame = 0

Query: 5   DPWINFKSARIPI-KVKDDFKNKRVNVLIEEDGS--WKESLIKEVFTSMEAEEIINMPRC 64
           D W+   +A + I    D  +N +V+ LI  +GS  W    +  +F  ++  + + +P  
Sbjct: 506 DKWVPRPAAFVVITSPLDGMENTKVSELICNEGSPQWDLQKLNNLFLPVDVVDFVRIPLS 565

Query: 65  KSKAKDEIIWNEDPKGQFTVKSAYHLATNLEARNE-ASSSDNQASTTIWNSIWKIKCIPR 124
                D I+WN D  G FTVKSAY +A  + + +E  SSS N  ++ +W  IW      +
Sbjct: 566 IRAPPDRIVWNYDKHGLFTVKSAYRVALRVTSGDEDESSSSNSDTSMLWRHIWNATVPTK 625

Query: 125 AKIAAWKIINDIIPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFSK-----KMLK 184
            KI AW++ +DI+P+  N+ +KG++  D C+F  +  E+  H++ MC F+       +L 
Sbjct: 626 LKIFAWRVAHDILPTKANLIKKGVDMQDMCMFCGDITESALHVLAMCPFAVATWNISLLT 685

Query: 185 KRRHQSRSAYYVELMEYEKHMLSQQSEPKFSEHQKINSKLYRRRRRKVDRAPDSDGRPVS 244
           +  HQ      V+   ++    +QQ   +F       SK+  R R  V  A  S GR   
Sbjct: 686 RHAHQG-----VQRSPHDVVGFAQQYVHEFITANDTPSKVTDRVRDPVRWAAPSSGRLKF 745

Query: 245 DSDGEPAESPPLIAWRWPIKVLE-------AKAISEGLKTYIKATEMEDLEIRPEMEV-- 304
           + DG  A  P   + R  + V+          A+++ +   + A   E L  R  + +  
Sbjct: 746 NFDG--AFDP--TSGRGAVGVVARDADGGFVAAVAKSVGEVLSAEHAEILAAREGVALAL 805

Query: 305 ---------EADSLEVIQTINKKSVDLSELSLVIDEIEEMVPGARVASFVKCSRSSNDIA 362
                    E DS  V+  + +   D S +  ++++++ +      + F    R +N + 
Sbjct: 806 SLGTASPIFEGDSAVVVSAVKRAGQDYSNIGTIVEDVKHLQQQFPSSLFQFTPREANGVV 865

BLAST of Lag0018532 vs. ExPASy TrEMBL
Match: M5VU98 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa022115mg PE=4 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.2e-21
Identity = 96/367 (26.16%), Postives = 168/367 (45.78%), Query Frame = 0

Query: 21   DDFKNKRVNVLIEEDGS--WKESLIKEVFTSMEAEEIINMPRCKSKAKDEIIWNEDPKGQ 80
            D  +N +V+ LI  +GS  W    +  +F  ++  +I+ +P       D I+WN D  G 
Sbjct: 1372 DGMENTKVSELICNEGSPQWDLQKLNNLFLPVDVVDIVRIPLSIRAPPDRIVWNYDKHGL 1431

Query: 81   FTVKSAYHLATNLEARNE-ASSSDNQASTTIWNSIWKIKCIPRAKIAAWKIINDIIPSNY 140
            FTVKSAY +A  + + +E  SSS N  +  +W  IW      + KI AW++ +DI+P+  
Sbjct: 1432 FTVKSAYRVALRVTSGDEDESSSSNSDTGMLWRHIWNATVPTKLKIFAWRVAHDILPTKA 1491

Query: 141  NVKRKGINSNDRCVFYREHEETTEHLIWMCKFSK-----KMLKKRRHQSRSAYYVELMEY 200
            N+ +KG++  D C+F  +  E+  H++ MC F+       +L +  HQ      V+   +
Sbjct: 1492 NLIKKGVDMQDMCMFCGDITESALHVLAMCPFAVATWNISLLTRHAHQG-----VQRSPH 1551

Query: 201  EKHMLSQQSEPKFSEHQKINSKLYRRRRRKVDRAPDSDGRPVSDSDGEPAESPPLIAWRW 260
            E    +QQ   +F       SK+  R R  V  A    GR   + DG  A  P   + R 
Sbjct: 1552 EVVGFAQQYVHEFITANDTPSKVTDRVRDPVRWAAPPSGRLKFNFDG--AFDP--TSGRG 1611

Query: 261  PIKVLE-------AKAISEGLKTYIKATEMEDLEIRPEMEV-----------EADSLEVI 320
             + V+          A+++ +   + A   E L  R  + +           E DS  V+
Sbjct: 1612 AVGVVARDADGGFVAAVAKSVGEVLSAEHAEILAAREGVALALSLGTASPIFEGDSAVVV 1671

Query: 321  QTINKKSVDLSELSLVIDEIEEMVPGARVASFVKCSRSSNDIAHKIARAGVIHGDFCYFF 362
              I +   D S +  ++++++ +      + F    R +N +AH++AR G+ + D   +F
Sbjct: 1672 SAIKRAGQDYSNIGTIVEDVKHLQQQFPSSLFQFTPREANGVAHRLARFGLHNVDNFIWF 1729

BLAST of Lag0018532 vs. ExPASy TrEMBL
Match: M5XHI9 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa019733mg PE=4 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 8.9e-21
Identity = 94/367 (25.61%), Postives = 167/367 (45.50%), Query Frame = 0

Query: 21   DDFKNKRVNVLIEEDGS--WKESLIKEVFTSMEAEEIINMPRCKSKAKDEIIWNEDPKGQ 80
            D  +N +V+ LI  +GS  W    +  +F  ++  +I+ +P       D I+WN D  G 
Sbjct: 892  DGMENTKVSELICNEGSPQWDLQKLNNLFLPVDVVDIVRIPLSIRAPPDRIVWNYDKHGL 951

Query: 81   FTVKSAYHLATNLEARNE-ASSSDNQASTTIWNSIWKIKCIPRAKIAAWKIINDIIPSNY 140
            FTVKSAY +A  + + +E  SSS N  +  +W  IW      + KI AW++ +DI+P+  
Sbjct: 952  FTVKSAYRVALRVTSGDEDESSSSNSDTGMLWRHIWNATVPTKLKIFAWRVAHDILPTKA 1011

Query: 141  NVKRKGINSNDRCVFYREHEETTEHLIWMCKFSK-----KMLKKRRHQSRSAYYVELMEY 200
            N+ +KG++  D C+F  +  E+  H++ MC F+       +L +  HQ      V+   +
Sbjct: 1012 NLIKKGVDMQDMCMFCGDITESALHVLAMCPFAVATWNISLLTRHAHQG-----VQRSPH 1071

Query: 201  EKHMLSQQSEPKFSEHQKINSKLYRRRRRKVDRAPDSDGRPVSDSDG--EPAESPPLIAW 260
            E    +QQ   +F       SK+  R R  V  A    GR   + DG  +P      +  
Sbjct: 1072 EVVGFAQQYVHEFITANDTPSKVTDRVRDPVRWAAPPSGRLKFNFDGAFDPTSGREAVG- 1131

Query: 261  RWPIKVLEA-----KAISEGLKTYIKATEMEDLEIRPEMEV-----------EADSLEVI 320
               +   +A      A+++ +   + A   E L  R  + +           E DS  V+
Sbjct: 1132 ---VVARDADGGFVAAVAKSVGEVLSAEHAEILVAREGVALALSLGTASPIFEGDSAVVV 1191

Query: 321  QTINKKSVDLSELSLVIDEIEEMVPGARVASFVKCSRSSNDIAHKIARAGVIHGDFCYFF 362
              I +   D S +  ++++++ +      + F    R +N +AH++AR G+ + D   +F
Sbjct: 1192 SAIKRAGQDYSNIGTIVEDVKHLQQQFPSSLFQFTPREANGVAHRLARFGLHNVDNFIWF 1249

BLAST of Lag0018532 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 72.8 bits (177), Expect = 6.7e-13
Identity = 39/158 (24.68%), Postives = 74/158 (46.84%), Query Frame = 0

Query: 16  PIKVKDDFKNKRVNVLIEEDGS---WKESLIKEVFTSMEAEEIINMPRCKSKAKDEIIWN 75
           P+  ++ +K   +N L E  GS   W +S I +     +   I  +   KSK  D+IIWN
Sbjct: 63  PLNTEETYKEMTINNLFERKGSYYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKIIWN 122

Query: 76  EDPKGQFTVKSAYHLATNLEARNEASSSDNQASTTIWNSIWKIKCIPRAKIAAWKIINDI 135
            +  G++TV+S Y L T+  + N  + +    S  +   IW +  +P+ K   W+ ++  
Sbjct: 123 YNTTGEYTVRSGYWLLTHDPSTNIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRALSQA 182

Query: 136 IPSNYNVKRKGINSNDRCVFYREHEETTEHLIWMCKFS 171
           + +   +  +G+  +  C       E+  H ++ C F+
Sbjct: 183 LATTERLTTRGMRIDPSCPRCHRENESINHALFTCPFA 220

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_040986468.11.4e-2036.55uncharacterized protein LOC121234565 [Juglans microcarpa x Juglans regia][more]
XP_023875263.11.2e-1932.95uncharacterized protein LOC111987752 [Quercus suber][more]
EEC72938.12.0e-1933.53hypothetical protein OsI_06800 [Oryza sativa Indica Group][more]
EEC84753.12.7e-1931.18hypothetical protein OsI_31756 [Oryza sativa Indica Group][more]
ABA98898.13.5e-1930.18retrotransposon protein, putative, unclassified [Oryza sativa Japonica Group][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2N9H3I82.1e-2234.41Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS34205 PE=4 SV=1[more]
A0A803PAX61.1e-2135.75Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
M5XK321.8e-2125.26Reverse transcriptase domain-containing protein (Fragment) OS=Prunus persica OX=... [more]
M5VU985.2e-2126.16Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRU... [more]
M5XHI98.9e-2125.61Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRU... [more]
Match NameE-valueIdentityDescription
AT3G09510.16.7e-1324.68Ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 79..172
e-value: 4.2E-16
score: 59.4
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 256..343
e-value: 6.1E-8
score: 32.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 222..237
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 216..243
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 36..172
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 36..172

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0018532.1Lag0018532.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity