Lag0017918 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017918
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionInositol oxygenase
Locationchr5: 11654078 .. 11654953 (+)
RNA-Seq ExpressionLag0017918
SyntenyLag0017918
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATGCGAATCACAAGGCGCCATGGATAGGTACCAGAAGGTCGAGAAGCCCAAGTTCATCTTCTTCCTGTTCAATCTCCTTACTCTTTGCTCCAGGTGTGTTTTCTGTAAATTCTCTCCTCTTTTTCCATTATGGTTTTGGAAATTTCTTTCATCTTTTTCTGTTATGGTTTATAGGATGAGTTACCCACACCTCATCTCTCCCGCTCCTCATCATTGTCGATATGCAAAAATGATGGGAAAACAATCTCTCAACTTCTCAATTTCTAAATCGAAGGGGTTGTCATGCGGAAAGCGAAGGGCAAGCAATTTCTGTTATTTTCATTACGATTTCGATTTAATTTCCTTACAATTTTGTAAGCAATCTTTTTTAATCGATTTAGTTTCCTTGCAAAAAGCAGAGATTCGTAATCGATTTAATTTCCTTACGATTTCGTAAGCATTTTCTGGGATTTCTACAAACTCAACCACATAAATCAAACTTACGATTTCGTAAGCAATTTCCTTTTTTCAAAATTCTGCTATTTTCATTACGACGTCGTCTCGTTCTTTCCTCCTCGCTCTACAGCTGAAATTCTCAAACAAATTGTTCTTCAAGAGATCATTGAAAATGGCGGAATTTGTTCTTACAGGCAAAGAGGATGAGGAAAGAGTACCACCAACACTGAACCGAGGAGAGATTGGCATATGGGAGTGTTGCAAGCTTCTCAATGACGTGGTTGATGAGAGCAATCCTGGTTGGGATGAGCCTCAAATCAAACACTTGTTGCAGAGCACTGAGGCCATCGCAGAGGATTATCCTAATGAAGATTGGTTGCATTTCACTGCTCTTATACATGGTTTTTATTCCTTTTCTTTGATGATTTCATTCTAG

mRNA sequence

ATGAGATGCGAATCACAAGGCGCCATGGATAGGTACCAGAAGGTCGAGAAGCCCAAGTTCATCTTCTTCCTGTTCAATCTCCTTACTCTTTGCTCCAGGATGAGTTACCCACACCTCATCTCTCCCGCTCCTCATCATTGTCGATATGCAAAAATGATGGGAAAACAATCTCTCAACTTCTCAATTTCTAAATCGAAGGGGTTGTCATGCGGAAAGCGAAGGGCAAGCAATTTCTGTTATTTTCATTACGATTTCGATTTAATTTCCTTACAATTTTGCAAAGAGGATGAGGAAAGAGTACCACCAACACTGAACCGAGGAGAGATTGGCATATGGGAGTGTTGCAAGCTTCTCAATGACGTGGTTGATGAGAGCAATCCTGGTTGGGATGAGCCTCAAATCAAACACTTGTTGCAGAGCACTGAGGCCATCGCAGAGGATTATCCTAATGAAGATTGGTTGCATTTCACTGCTCTTATACATGGTTTTTATTCCTTTTCTTTGATGATTTCATTCTAG

Coding sequence (CDS)

ATGAGATGCGAATCACAAGGCGCCATGGATAGGTACCAGAAGGTCGAGAAGCCCAAGTTCATCTTCTTCCTGTTCAATCTCCTTACTCTTTGCTCCAGGATGAGTTACCCACACCTCATCTCTCCCGCTCCTCATCATTGTCGATATGCAAAAATGATGGGAAAACAATCTCTCAACTTCTCAATTTCTAAATCGAAGGGGTTGTCATGCGGAAAGCGAAGGGCAAGCAATTTCTGTTATTTTCATTACGATTTCGATTTAATTTCCTTACAATTTTGCAAAGAGGATGAGGAAAGAGTACCACCAACACTGAACCGAGGAGAGATTGGCATATGGGAGTGTTGCAAGCTTCTCAATGACGTGGTTGATGAGAGCAATCCTGGTTGGGATGAGCCTCAAATCAAACACTTGTTGCAGAGCACTGAGGCCATCGCAGAGGATTATCCTAATGAAGATTGGTTGCATTTCACTGCTCTTATACATGGTTTTTATTCCTTTTCTTTGATGATTTCATTCTAG

Protein sequence

MRCESQGAMDRYQKVEKPKFIFFLFNLLTLCSRMSYPHLISPAPHHCRYAKMMGKQSLNFSISKSKGLSCGKRRASNFCYFHYDFDLISLQFCKEDEERVPPTLNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIHGFYSFSLMISF
Homology
BLAST of Lag0017918 vs. NCBI nr
Match: KAA3463230.1 (inositol oxygenase 4-like isoform X1 [Gossypium australe])

HSP 1 Score: 109.0 bits (271), Expect = 4.1e-20
Identity = 50/79 (63.29%), Postives = 63/79 (79.75%), Query Frame = 0

Query: 83  YDFDLISLQFCKEDEERVPPTLNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTE 142
           YDF    ++  +E+ E+    LN+GE+GIWECC+LLN+VVD+S+P  DEPQI+HLLQS E
Sbjct: 69  YDF----VKKIREEYEK----LNKGEMGIWECCELLNEVVDDSDPDLDEPQIQHLLQSAE 128

Query: 143 AIAEDYPNEDWLHFTALIH 162
           AI +DYPNEDWLH TALIH
Sbjct: 129 AIRKDYPNEDWLHLTALIH 139

BLAST of Lag0017918 vs. NCBI nr
Match: KAG7018305.1 (Inositol oxygenase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 108.6 bits (270), Expect = 5.4e-20
Identity = 48/66 (72.73%), Postives = 55/66 (83.33%), Query Frame = 0

Query: 104 LNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIHGF 163
           LNR E+ IWECC+LLNDVVDES+P  DEPQI+HLLQ+ EAI +DYPNEDWLH TALIHG 
Sbjct: 89  LNRVEMSIWECCELLNDVVDESDPDLDEPQIEHLLQTAEAIRKDYPNEDWLHLTALIHGS 148

Query: 164 YSFSLM 170
             F L+
Sbjct: 149 NDFILL 154

BLAST of Lag0017918 vs. NCBI nr
Match: XP_027343206.1 (inositol oxygenase 2-like [Abrus precatorius])

HSP 1 Score: 107.1 bits (266), Expect = 1.6e-19
Identity = 47/69 (68.12%), Postives = 57/69 (82.61%), Query Frame = 0

Query: 104 LNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIHGF 163
           L++GE+GIWECC+LLN+VVD+S+P  DEPQI+HLLQS EAI +DYPNEDWLH TALIH  
Sbjct: 95  LDKGEMGIWECCELLNEVVDDSDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIHDL 154

Query: 164 YSFSLMISF 173
               L+ SF
Sbjct: 155 GKILLLPSF 163

BLAST of Lag0017918 vs. NCBI nr
Match: KAF7126643.1 (hypothetical protein RHSIM_Rhsim11G0048700 [Rhododendron simsii])

HSP 1 Score: 106.7 bits (265), Expect = 2.0e-19
Identity = 48/71 (67.61%), Postives = 55/71 (77.46%), Query Frame = 0

Query: 91  QFCKEDEERVPPTLNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPN 150
           QF K+ +E     LNR E+ IWECC+LLN+ VDES+P  DEPQI+HLLQS EAI  DYPN
Sbjct: 56  QFAKKKKEEY-GKLNRAEMSIWECCELLNEFVDESDPDLDEPQIEHLLQSAEAIRRDYPN 115

Query: 151 EDWLHFTALIH 162
           EDWLH TALIH
Sbjct: 116 EDWLHLTALIH 125

BLAST of Lag0017918 vs. NCBI nr
Match: KAF9663871.1 (hypothetical protein SADUNF_Sadunf17G0097100 [Salix dunnii])

HSP 1 Score: 106.7 bits (265), Expect = 2.0e-19
Identity = 46/61 (75.41%), Postives = 52/61 (85.25%), Query Frame = 0

Query: 104 LNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIHGF 163
           LN+ E+ IWECC+LLNDVVDES+P  DEPQI HLLQS EAI +DYP+EDWLH TALIHG 
Sbjct: 90  LNKAEMSIWECCELLNDVVDESDPDLDEPQIMHLLQSAEAIRKDYPDEDWLHLTALIHGT 149

Query: 164 Y 165
           Y
Sbjct: 150 Y 150

BLAST of Lag0017918 vs. ExPASy Swiss-Prot
Match: O82200 (Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2)

HSP 1 Score: 98.6 bits (244), Expect = 7.3e-20
Identity = 44/69 (63.77%), Postives = 53/69 (76.81%), Query Frame = 0

Query: 104 LNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIHGF 163
           LN+ E+ IWECC+LLN+VVDES+P  DEPQI+HLLQ+ EAI  DYP+EDWLH TALIH  
Sbjct: 96  LNKMEMSIWECCELLNNVVDESDPDLDEPQIQHLLQTAEAIRRDYPDEDWLHLTALIHDL 155

Query: 164 YSFSLMISF 173
               L+  F
Sbjct: 156 GKVLLLPEF 164

BLAST of Lag0017918 vs. ExPASy Swiss-Prot
Match: Q8H1S0 (Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 7.3e-20
Identity = 42/58 (72.41%), Postives = 50/58 (86.21%), Query Frame = 0

Query: 104 LNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIH 162
           L++  + IWECC+LLN+VVDES+P  DEPQI+HLLQS EAI +DYPNEDWLH TALIH
Sbjct: 96  LDKMVMSIWECCELLNEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIH 153

BLAST of Lag0017918 vs. ExPASy Swiss-Prot
Match: Q8L799 (Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 1.6e-19
Identity = 43/69 (62.32%), Postives = 52/69 (75.36%), Query Frame = 0

Query: 104 LNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIHGF 163
           LNR E+ IWECC+LLN+ +DES+P  DEPQI+HLLQ+ EAI +DYP+EDWLH T LIH  
Sbjct: 90  LNRTEMSIWECCELLNEFIDESDPDLDEPQIEHLLQTAEAIRKDYPDEDWLHLTGLIHDL 149

Query: 164 YSFSLMISF 173
               L  SF
Sbjct: 150 GKVLLHSSF 158

BLAST of Lag0017918 vs. ExPASy Swiss-Prot
Match: Q9FJU4 (Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 1.8e-18
Identity = 40/58 (68.97%), Postives = 48/58 (82.76%), Query Frame = 0

Query: 104 LNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTEAIAEDYPNEDWLHFTALIH 162
           L++  + IWECC+L  +VVDES+P  DEPQI+HLLQS EAI +DYPNEDWLH TALIH
Sbjct: 93  LDKMVMNIWECCELSKEVVDESDPDLDEPQIQHLLQSAEAIRKDYPNEDWLHLTALIH 150

BLAST of Lag0017918 vs. ExPASy Swiss-Prot
Match: Q5Z8T3 (Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g0561000 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.3e-16
Identity = 44/90 (48.89%), Postives = 59/90 (65.56%), Query Frame = 0

Query: 83  YDFDLISLQFCKEDEERVPPTLNRGEIGIWECCKLLNDVVDESNPGWDEPQIKHLLQSTE 142
           YDF    ++  +E+  RV  T    E+GIWEC +LLN+ +D+S+P  D PQI+HLLQ+ E
Sbjct: 74  YDF----VRRMREEYGRVDKT----EMGIWECIELLNEFIDDSDPDLDMPQIEHLLQTAE 133

Query: 143 AIAEDYPNEDWLHFTALIHGFYSFSLMISF 173
           AI +D+P+EDWLH T LIH      L  SF
Sbjct: 134 AIRKDFPDEDWLHLTGLIHDLGKVLLHPSF 155

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA3463230.14.1e-2063.29inositol oxygenase 4-like isoform X1 [Gossypium australe][more]
KAG7018305.15.4e-2072.73Inositol oxygenase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_027343206.11.6e-1968.12inositol oxygenase 2-like [Abrus precatorius][more]
KAF7126643.12.0e-1967.61hypothetical protein RHSIM_Rhsim11G0048700 [Rhododendron simsii][more]
KAF9663871.12.0e-1975.41hypothetical protein SADUNF_Sadunf17G0097100 [Salix dunnii][more]
Match NameE-valueIdentityDescription
O822007.3e-2063.77Inositol oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=MIOX2 PE=2 SV=2[more]
Q8H1S07.3e-2072.41Inositol oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=MIOX4 PE=2 SV=1[more]
Q8L7991.6e-1962.32Inositol oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=MIOX1 PE=2 SV=1[more]
Q9FJU41.8e-1868.97Inositol oxygenase 5 OS=Arabidopsis thaliana OX=3702 GN=MIOX5 PE=2 SV=1[more]
Q5Z8T31.3e-1648.89Probable inositol oxygenase OS=Oryza sativa subsp. japonica OX=39947 GN=Os06g056... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007828Inositol oxygenasePFAMPF05153MIOXcoord: 91..162
e-value: 2.5E-19
score: 69.8
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 103..167
IPR007828Inositol oxygenasePANTHERPTHR12588MYOINOSITOL OXYGENASEcoord: 102..163
NoneNo IPR availablePANTHERPTHR12588:SF9INOSITOL OXYGENASE 4-LIKE ISOFORM X1coord: 102..163
NoneNo IPR availablePANTHERPTHR12588:SF14INOSITOL OXYGENASE 5coord: 103..167
NoneNo IPR availableSUPERFAMILY109604HD-domain/PDEase-likecoord: 103..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017918.1Lag0017918.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019310 inositol catabolic process
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0050113 inositol oxygenase activity
molecular_function GO:0005506 iron ion binding