Lag0017609 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0017609 vs. NCBI nr
Match: XP_038876807.1 (ACT domain-containing protein ACR8-like [Benincasa hispida])

HSP 1 Score: 831.6 bits (2147), Expect = 3.1e-237
Identity = 421/445 (94.61%), Postives = 429/445 (96.40%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL
Sbjct: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRR+E FNG TT 
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRNEEFNGTTTT 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSG PIED+QKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIEDQQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
            I+ARLRSVLKGDNDIRSAKT VSMAVTHTERRLHQMMFADRDYERKPILKSNA++SP V
Sbjct: 181 TIVARLRSVLKGDNDIRSAKTLVSMAVTHTERRLHQMMFADRDYERKPILKSNANNSPTV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQNC ERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD
Sbjct: 241 TVQNCAERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCT DRPGLLADV RTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTTDRPGLLADVMRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DMALNAFYVTDV+G+AADPK IESVRQRIGLSNL VKE PSTYHQT EREEQ LG
Sbjct: 361 ISTTRDMALNAFYVTDVVGNAADPKTIESVRQRIGLSNLKVKELPSTYHQTTEREEQTLG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLIK+
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIKS 445

BLAST of Lag0017609 vs. NCBI nr
Match: XP_022946673.1 (ACT domain-containing protein ACR8 [Cucurbita moschata] >KAG7028357.1 ACT domain-containing protein ACR8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 828.6 bits (2139), Expect = 2.6e-236
Identity = 418/445 (93.93%), Postives = 432/445 (97.08%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMME+EWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQ+LTDL
Sbjct: 1   MMMEVEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQILTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTD+NGEKLTDESVISYLEQSLG T+YR SEGFNGATTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDKNGEKLTDESVISYLEQSLGMTNYRSSEGFNGATTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTH+GRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHSGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
           II+ARLRSVLKGDNDIRSAKTSVSMAVTH ERRLHQMMFADRDYERKPI KSNA++SPAV
Sbjct: 181 IIVARLRSVLKGDNDIRSAKTSVSMAVTHPERRLHQMMFADRDYERKPIFKSNANNSPAV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD
Sbjct: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCL+AAIQRRASEGV+LELCT DRPGLLADVTRTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLKAAIQRRASEGVKLELCTTDRPGLLADVTRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DM LN FYVTD IGSAADPKIIESVRQRIGLSNLIVKE PS+YHQT+E+EEQ  G
Sbjct: 361 ISTTDDMVLNTFYVTDAIGSAADPKIIESVRQRIGLSNLIVKELPSSYHQTIEKEEQTHG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLIK+
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIKS 445

BLAST of Lag0017609 vs. NCBI nr
Match: XP_023539972.1 (ACT domain-containing protein ACR8 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 828.2 bits (2138), Expect = 3.5e-236
Identity = 417/445 (93.71%), Postives = 432/445 (97.08%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMME+EWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQ+LTDL
Sbjct: 1   MMMEVEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQILTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTD+NGEKLTDESVISYLEQSLG T+YR SEGFNGATTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDKNGEKLTDESVISYLEQSLGMTNYRSSEGFNGATTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTH+GRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHSGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
           II+ARLRSVLKGDNDIRSAKTSVSMAVTH ERRLHQMMFADRDYERKPI KSNA++SPAV
Sbjct: 181 IIVARLRSVLKGDNDIRSAKTSVSMAVTHPERRLHQMMFADRDYERKPIFKSNANNSPAV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD
Sbjct: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCL+AAIQRRASEGV+LELCT DRPGLLADVTRTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLKAAIQRRASEGVKLELCTTDRPGLLADVTRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DM LN FYVTD +GSAADPKIIES+RQRIGLSNLIVKE PS+YHQT+EREEQ  G
Sbjct: 361 ISTTDDMVLNTFYVTDAMGSAADPKIIESIRQRIGLSNLIVKELPSSYHQTIEREEQTHG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLIK+
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIKS 445

BLAST of Lag0017609 vs. NCBI nr
Match: XP_023005406.1 (ACT domain-containing protein ACR8-like [Cucurbita maxima])

HSP 1 Score: 827.0 bits (2135), Expect = 7.7e-236
Identity = 417/445 (93.71%), Postives = 431/445 (96.85%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMME+EWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQ+LTDL
Sbjct: 1   MMMEVEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQILTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTD+NGEKLTDESVISYLEQSLG T+YR SEGFNG TTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDKNGEKLTDESVISYLEQSLGMTNYRSSEGFNGTTTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTH+GRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHSGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
           II+ARLRSVLKGDNDIRSAKTSVSMAVTH ERRLHQMMFADRDYERKPI KSNA++SPAV
Sbjct: 181 IIVARLRSVLKGDNDIRSAKTSVSMAVTHPERRLHQMMFADRDYERKPIFKSNANNSPAV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD
Sbjct: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCL+AAIQRRASEGV+LELCT DRPGLLADVTRTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLKAAIQRRASEGVKLELCTTDRPGLLADVTRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DM LN FYVTD +GSAADPKIIESVRQRIGLSNLIVKE PS+YHQT+EREEQ  G
Sbjct: 361 ISTTDDMVLNTFYVTDAMGSAADPKIIESVRQRIGLSNLIVKELPSSYHQTIEREEQTHG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLIK+
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIKS 445

BLAST of Lag0017609 vs. NCBI nr
Match: XP_016900581.1 (PREDICTED: ACT domain-containing protein ACR8-like [Cucumis melo])

HSP 1 Score: 817.8 bits (2111), Expect = 4.7e-233
Identity = 413/445 (92.81%), Postives = 425/445 (95.51%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNG CSTATLVKVDSARR+GNLLEAVQVLTDL
Sbjct: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGTCSTATLVKVDSARRFGNLLEAVQVLTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISY+EQSLGTTHYRR+E FNG TTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYIEQSLGTTHYRRNEEFNGTTTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
            I+ARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILK NAD+SP V
Sbjct: 181 TIVARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKLNADNSPTV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           +VQNC ERGYSVV VQCKDRTKLLFDVIFTLTDMEYVVFHATINT++ERAYLEFYIRHSD
Sbjct: 241 SVQNCAERGYSVVCVQCKDRTKLLFDVIFTLTDMEYVVFHATINTSQERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCT DRPGLLADV RTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTEDRPGLLADVMRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DMALN FYVTDV+G+AAD K IESVRQRIGLSNL VKE PSTYHQT EREEQ  G
Sbjct: 361 ISTTRDMALNVFYVTDVVGNAADQKTIESVRQRIGLSNLKVKELPSTYHQTTEREEQTFG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLI++
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIRS 445

BLAST of Lag0017609 vs. ExPASy Swiss-Prot
Match: Q9LNA5 (ACT domain-containing protein ACR8 OS=Arabidopsis thaliana OX=3702 GN=ACR8 PE=2 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 5.0e-161
Identity = 300/437 (68.65%), Postives = 351/437 (80.32%), Query Frame = 0

Query: 11  LDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVS 70
           LDEYEKLVIRMNTPRVVIDNG CS+AT+VKVDS+RR G LLEAVQ+LTDLNLSIKKAY+S
Sbjct: 7   LDEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSIKKAYIS 66

Query: 71  SDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTALELTGTDRVG 130
           SDG W MDVFHVTD NG KL D+SV+ Y+EQS+ T +Y  +   NG  TALELTGTDR+G
Sbjct: 67  SDGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSIETVYYGENIEVNG-LTALELTGTDRIG 126

Query: 131 LLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDIIIARLRSVL 190
           LLSE+FAVL+DL CDVV+AK+WTHNGR+AS+IY+KDC SG PI D  +I  I  RL++VL
Sbjct: 127 LLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGRLKNVL 186

Query: 191 KGDNDIRS-AKTSVSM-AVTHTERRLHQMMFADRDYERKPILKSNADSSPAVTVQNCTER 250
            GDND+ S AKT V++ ++ H ERRLHQ+MF DRDYER+   K        VTVQN  ER
Sbjct: 187 NGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYERRS-KKHERSPMVVVTVQNWAER 246

Query: 251 GYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSDGTPISSEA 310
           GYSVV V C+DRTKLLFDV+ TLTDMEY VFHATINTAE++A+LEFYIRH DG+PISSEA
Sbjct: 247 GYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHKDGSPISSEA 306

Query: 311 ERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAEISTTGDMA 370
           ERQRVIQCL+AA++RRA EGVRLEL   D+ GLLA+VTRTFRENGLNVTR EIST+ DMA
Sbjct: 307 ERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRTEISTSSDMA 366

Query: 371 LNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALGVGGAVLFT 430
            N FYVTD  G   D K+IESVR++IGL  L VKE P+ YH+  + EEQ      AVL +
Sbjct: 367 TNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQ--QTKAVLVS 426

Query: 431 LGSMVRRNLYNLGLIKA 446
           LGS+V RNL+N GLIK+
Sbjct: 427 LGSLVWRNLFNFGLIKS 439

BLAST of Lag0017609 vs. ExPASy Swiss-Prot
Match: Q8LJW1 (ACT domain-containing protein ACR7 OS=Arabidopsis thaliana OX=3702 GN=ACR7 PE=2 SV=1)

HSP 1 Score: 543.5 bits (1399), Expect = 2.2e-153
Identity = 284/452 (62.83%), Postives = 352/452 (77.88%), Query Frame = 0

Query: 5   MEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSI 64
           ME   C +EYEKLV+RMN PRVVIDNG C  +T+VK+DSAR  G LLE+VQ+LTD+NL I
Sbjct: 1   MELSDCSNEYEKLVVRMNMPRVVIDNGVCPNSTVVKIDSARSPGILLESVQLLTDMNLWI 60

Query: 65  KKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTALELT 124
           KKAY+SSDG+W MDVFHV+D NG+KLTDE++I Y+E+S+ T+HY ++EG+ G  TALELT
Sbjct: 61  KKAYISSDGKWNMDVFHVSDLNGDKLTDENLIRYIEKSIETSHYCKTEGYTG-LTALELT 120

Query: 125 GTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIE-DRQKIDIII 184
           GTDRVGLLSEVFAVLADL+CDVVEAK WTHNGRIAS+IYVKD NSG PI+ D  ++  + 
Sbjct: 121 GTDRVGLLSEVFAVLADLECDVVEAKAWTHNGRIASMIYVKDGNSGTPIDGDSDRVQRVE 180

Query: 185 ARLRSVLKGDNDIRS-AKTSVSM-AVTHTERRLHQMMFADRDYERKPILKSNADSSPAVT 244
            +LR++LK D+  ++  +T VS    TH ERRLHQ MF DRDYE+    K + + SP V+
Sbjct: 181 GQLRNLLKADDGYQNDTRTCVSYGGNTHMERRLHQRMFMDRDYEK----KFDIEKSPIVS 240

Query: 245 VQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSDG 304
           VQN  +RGYSVV +QCKDR KLLFDV+ TLTDM Y+VFHA I T  E A+LEFY+RHSDG
Sbjct: 241 VQNLPKRGYSVVNLQCKDRMKLLFDVVCTLTDMAYIVFHAAIRTVGETAFLEFYVRHSDG 300

Query: 305 TPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAEI 364
            P+SSE ERQR+IQCLQAAI+RR  +GVRLELCTADRPGLLA+VTR  RENGLN+ RAEI
Sbjct: 301 HPVSSEPERQRLIQCLQAAIERRTVKGVRLELCTADRPGLLAEVTRILRENGLNIARAEI 360

Query: 365 STTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEA-PSTYHQTMEREE---- 424
           ST   +A N FYVTD  G+  DP+II+S+R++IG+ +L VKE  P +  + +E+E+    
Sbjct: 361 STKDSIARNVFYVTDANGNLIDPEIIKSIREKIGIDDLSVKEPFPISCREAVEKEQHIEP 420

Query: 425 ---QALGVGGAVLFTLGSMVRRNLYNLGLIKA 446
              Q    GG VL +LGS+V RNLY+LGLIK+
Sbjct: 421 QDHQGRYGGGTVLVSLGSLVMRNLYHLGLIKS 447

BLAST of Lag0017609 vs. ExPASy Swiss-Prot
Match: Q8LJW3 (ACT domain-containing protein ACR4 OS=Arabidopsis thaliana OX=3702 GN=ACR4 PE=2 SV=1)

HSP 1 Score: 442.6 bits (1137), Expect = 5.4e-123
Identity = 237/448 (52.90%), Postives = 311/448 (69.42%), Query Frame = 0

Query: 12  DEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVSS 71
           +EYEKL+ RMN PRVVIDN +C  AT+++VDSA  YG LLE VQ+LTDLNL+I KAY+SS
Sbjct: 9   NEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTITKAYISS 68

Query: 72  DGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGT----THYRRSEGFNGAT--TALELTG 131
           DG WFMDVF+VTDQ+G K+TDE V+ Y+++SLG     +   RS G   +T  T +ELTG
Sbjct: 69  DGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDSTVIELTG 128

Query: 132 TDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDIIIAR 191
            DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +GC I D +++  I   
Sbjct: 129 CDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPERLSRIKNL 188

Query: 192 LRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSS-------P 251
           LR+VLKG N  R AKT VS    HT+RRLHQMMF DRDYE + +   + DSS       P
Sbjct: 189 LRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLV---DDDSSIQDERQRP 248

Query: 252 AVTVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRH 311
            V V N  ++ YSVV V+CKDR KLLFD + TLTDM+YVVFH +++T    A+ E+Y+RH
Sbjct: 249 DVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEYYVRH 308

Query: 312 SDGTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTR 371
            DG+P+ SEAE+QRVIQCL+AAI+RR SEG++LELCT DR GLL++VTR FREN L VTR
Sbjct: 309 IDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSLTVTR 368

Query: 372 AEISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQA 431
           AE+ T G  ALN FYV+D  G + D K I+S+RQ IG + L VK  P    Q  ++ +++
Sbjct: 369 AEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNP----QEQQQRQKS 428

Query: 432 LGVGGAVLFTLGSMVR-RNLYNLGLIKA 446
                   F  G + + ++  N GL+++
Sbjct: 429 PSHESPTRFLFGGLFKSKSFVNFGLVRS 449

BLAST of Lag0017609 vs. ExPASy Swiss-Prot
Match: Q9ZPQ8 (ACT domain-containing protein ACR5 OS=Arabidopsis thaliana OX=3702 GN=ACR5 PE=2 SV=2)

HSP 1 Score: 416.0 bits (1068), Expect = 5.4e-115
Identity = 232/455 (50.99%), Postives = 299/455 (65.71%), Query Frame = 0

Query: 12  DEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVSS 71
           DE  K + R+N PRVVIDN  C   T++KVDSA ++G LLE VQVLT+LNL+IKKAY+SS
Sbjct: 13  DEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNLTIKKAYISS 72

Query: 72  DGRWFMDVFHVTDQNGEKLTDESVISYLEQSLG-------TTHYRRSEGFNGAT--TALE 131
           DG WFMDVF+VTDQ+G K+TDE V+ Y+ +SLG       +   R + G   +   T +E
Sbjct: 73  DGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVKQSVDYTVVE 132

Query: 132 LTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDII 191
           LTGTDR GLLSE+ AVL DLQC+VV A++WTH  + A+++ V D  +   I D +++  I
Sbjct: 133 LTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAITDPERLSKI 192

Query: 192 IARLRSVLKGDND---IRSAKTSVSMAV--THTERRLHQMMFADRDYERKPILKSNADSS 251
              L  VL G +     R  KT+VS A+  THT+R+LHQ+MFADRDY+     ++N D  
Sbjct: 193 RKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDE---WENNVDDE 252

Query: 252 -------PAVTVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERA 311
                  P V V N  +  YS+V ++CKDR KLLFD +FTLTDM YVV HA+I+    +A
Sbjct: 253 DKCGRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHASIDAEGPQA 312

Query: 312 YLEFYIRHSDGTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFR 371
           Y E+YIRH+DG+P+ SEAERQRVI+CL+AAIQRR SEG++LELCT+DR GLL+DVTR FR
Sbjct: 313 YQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGLLSDVTRIFR 372

Query: 372 ENGLNVTRAEISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQ 431
           EN L VTRAE+ T GD ALN FYV D  G   D K IES+RQ IG + L VK   +    
Sbjct: 373 ENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQVKGGNTDAKP 432

Query: 432 TMEREEQALGVGGAVLFTLGSMVRRNLYNLGLIKA 446
           + +             F  G    R+  N GLI++
Sbjct: 433 SPQDSPTG--------FLFGVFKSRSFVNFGLIRS 456

BLAST of Lag0017609 vs. ExPASy Swiss-Prot
Match: Q9SGA0 (ACT domain-containing protein ACR6 OS=Arabidopsis thaliana OX=3702 GN=ACR6 PE=1 SV=1)

HSP 1 Score: 406.8 bits (1044), Expect = 3.3e-112
Identity = 218/400 (54.50%), Postives = 285/400 (71.25%), Query Frame = 0

Query: 12  DEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVSS 71
           DEY KL+ RMN PRVVIDN A   AT+++VDS  ++G LLE VQVLTD+NL IKKAY+SS
Sbjct: 4   DEYAKLIRRMNPPRVVIDNNASDDATVIQVDSVNKHGTLLEVVQVLTDMNLVIKKAYISS 63

Query: 72  DGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTT------HYRRSEGF--NGATTALEL 131
           DG WFMDVF V DQ+G K+ D  V+ Y+++ + +         R S G       T++EL
Sbjct: 64  DGGWFMDVFKVIDQDGNKIRDTQVLDYIQKRIESNAGWFIPPLRSSVGVMPTDEYTSIEL 123

Query: 132 TGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDIII 191
            GTDR GLLSEV AVL DL C+VV A++WTHN R A++I+V D ++   I D  ++  I 
Sbjct: 124 AGTDRPGLLSEVSAVLTDLHCNVVNAEIWTHNTRAAAVIHVTDNSTHSAITDPIRLSTIK 183

Query: 192 ARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAVTVQ 251
             L +V++ ++  R+AKT  S + TH ERRLHQ+MF DRDYE     +++A S P+VT+ 
Sbjct: 184 ELLCNVVRTNSGSRAAKTVFSCSDTHRERRLHQIMFDDRDYEGVKRARTSA-SRPSVTLM 243

Query: 252 NCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSDGTP 311
           N  E+ Y+VV ++ KDR KL+FDV+ TLTDM+YVVFH  ++T    AY EFYIRH DG P
Sbjct: 244 N-IEKDYTVVTMRSKDRPKLVFDVVCTLTDMQYVVFHGMVSTEPVEAYQEFYIRHVDGLP 303

Query: 312 ISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAEIST 371
           I+SEAE++RVIQCL+AAI+RRASEG+ LEL   DR GLL+D+TRTFREN L + RAEIST
Sbjct: 304 INSEAEQERVIQCLEAAIERRASEGLELELSAEDRVGLLSDITRTFRENSLTIVRAEIST 363

Query: 372 TGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKE 404
               A + FYVTDV G+  + KI+ES+RQ+IG+S L VK+
Sbjct: 364 REGKAKDTFYVTDVTGNPVESKIVESIRQQIGVSKLKVKK 401

BLAST of Lag0017609 vs. ExPASy TrEMBL
Match: A0A6J1G4H7 (ACT domain-containing protein ACR8 OS=Cucurbita moschata OX=3662 GN=LOC111450642 PE=4 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.3e-236
Identity = 418/445 (93.93%), Postives = 432/445 (97.08%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMME+EWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQ+LTDL
Sbjct: 1   MMMEVEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQILTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTD+NGEKLTDESVISYLEQSLG T+YR SEGFNGATTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDKNGEKLTDESVISYLEQSLGMTNYRSSEGFNGATTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTH+GRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHSGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
           II+ARLRSVLKGDNDIRSAKTSVSMAVTH ERRLHQMMFADRDYERKPI KSNA++SPAV
Sbjct: 181 IIVARLRSVLKGDNDIRSAKTSVSMAVTHPERRLHQMMFADRDYERKPIFKSNANNSPAV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD
Sbjct: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCL+AAIQRRASEGV+LELCT DRPGLLADVTRTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLKAAIQRRASEGVKLELCTTDRPGLLADVTRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DM LN FYVTD IGSAADPKIIESVRQRIGLSNLIVKE PS+YHQT+E+EEQ  G
Sbjct: 361 ISTTDDMVLNTFYVTDAIGSAADPKIIESVRQRIGLSNLIVKELPSSYHQTIEKEEQTHG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLIK+
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIKS 445

BLAST of Lag0017609 vs. ExPASy TrEMBL
Match: A0A6J1KUW1 (ACT domain-containing protein ACR8-like OS=Cucurbita maxima OX=3661 GN=LOC111498405 PE=4 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 3.7e-236
Identity = 417/445 (93.71%), Postives = 431/445 (96.85%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMME+EWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQ+LTDL
Sbjct: 1   MMMEVEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQILTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTD+NGEKLTDESVISYLEQSLG T+YR SEGFNG TTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDKNGEKLTDESVISYLEQSLGMTNYRSSEGFNGTTTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTH+GRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHSGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
           II+ARLRSVLKGDNDIRSAKTSVSMAVTH ERRLHQMMFADRDYERKPI KSNA++SPAV
Sbjct: 181 IIVARLRSVLKGDNDIRSAKTSVSMAVTHPERRLHQMMFADRDYERKPIFKSNANNSPAV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD
Sbjct: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCL+AAIQRRASEGV+LELCT DRPGLLADVTRTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLKAAIQRRASEGVKLELCTTDRPGLLADVTRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DM LN FYVTD +GSAADPKIIESVRQRIGLSNLIVKE PS+YHQT+EREEQ  G
Sbjct: 361 ISTTDDMVLNTFYVTDAMGSAADPKIIESVRQRIGLSNLIVKELPSSYHQTIEREEQTHG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLIK+
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIKS 445

BLAST of Lag0017609 vs. ExPASy TrEMBL
Match: A0A1S4DX74 (ACT domain-containing protein ACR8-like OS=Cucumis melo OX=3656 GN=LOC103490944 PE=4 SV=1)

HSP 1 Score: 817.8 bits (2111), Expect = 2.3e-233
Identity = 413/445 (92.81%), Postives = 425/445 (95.51%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNG CSTATLVKVDSARR+GNLLEAVQVLTDL
Sbjct: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGTCSTATLVKVDSARRFGNLLEAVQVLTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISY+EQSLGTTHYRR+E FNG TTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYIEQSLGTTHYRRNEEFNGTTTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
            I+ARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILK NAD+SP V
Sbjct: 181 TIVARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKLNADNSPTV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           +VQNC ERGYSVV VQCKDRTKLLFDVIFTLTDMEYVVFHATINT++ERAYLEFYIRHSD
Sbjct: 241 SVQNCAERGYSVVCVQCKDRTKLLFDVIFTLTDMEYVVFHATINTSQERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCT DRPGLLADV RTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTEDRPGLLADVMRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DMALN FYVTDV+G+AAD K IESVRQRIGLSNL VKE PSTYHQT EREEQ  G
Sbjct: 361 ISTTRDMALNVFYVTDVVGNAADQKTIESVRQRIGLSNLKVKELPSTYHQTTEREEQTFG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLI++
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIRS 445

BLAST of Lag0017609 vs. ExPASy TrEMBL
Match: A0A6J1DGW3 (ACT domain-containing protein ACR8-like OS=Momordica charantia OX=3673 GN=LOC111020379 PE=4 SV=1)

HSP 1 Score: 808.9 bits (2088), Expect = 1.1e-230
Identity = 412/445 (92.58%), Postives = 425/445 (95.51%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL
Sbjct: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHY+RSEGFNG TTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYQRSEGFNGVTTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVV+AKVWTHNGRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVDAKVWTHNGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
           II+ARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILK NAD SPAV
Sbjct: 181 IIVARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKLNAD-SPAV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQN TERGYSVV VQCKDRTKLLFDVIFTLTDMEYVVFHATI TAEERAYLEFYIRH+D
Sbjct: 241 TVQNWTERGYSVVNVQCKDRTKLLFDVIFTLTDMEYVVFHATIKTAEERAYLEFYIRHTD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSEAERQRVIQCLQAAIQRRASEG+RLELCT DRPGLLADVTRTFRENGLNVTRAE
Sbjct: 301 GTPISSEAERQRVIQCLQAAIQRRASEGMRLELCTTDRPGLLADVTRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           ISTT DMALNAFYVTD +G+ ADPK+IESVR RIG  +L VKE PS+YHQT +REE A G
Sbjct: 361 ISTTRDMALNAFYVTDAVGNPADPKLIESVRHRIGFGSLKVKELPSSYHQTADREEHAPG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VG AVLFTLGSMVRRNLYNLGLI++
Sbjct: 421 VGVAVLFTLGSMVRRNLYNLGLIRS 444

BLAST of Lag0017609 vs. ExPASy TrEMBL
Match: A0A6J1E5Z2 (ACT domain-containing protein ACR8-like OS=Cucurbita moschata OX=3662 GN=LOC111431030 PE=4 SV=1)

HSP 1 Score: 805.8 bits (2080), Expect = 8.9e-230
Identity = 407/445 (91.46%), Postives = 419/445 (94.16%), Query Frame = 0

Query: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDL 60
           MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQ+LTD+
Sbjct: 1   MMMEMEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQILTDI 60

Query: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTA 120
           NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTD++VI YLEQSLGTTHYRR+E FNGATTA
Sbjct: 61  NLSIKKAYVSSDGRWFMDVFHVTDQNGEKLTDDTVIRYLEQSLGTTHYRRNEAFNGATTA 120

Query: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKID 180
           LELTGTDRVGLLSEVFAVLADLQCDVVEAK+WTHNGRIASLIYVKDCNSG PIEDRQKID
Sbjct: 121 LELTGTDRVGLLSEVFAVLADLQCDVVEAKIWTHNGRIASLIYVKDCNSGSPIEDRQKID 180

Query: 181 IIIARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSSPAV 240
           II+ARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERK ILKSNA  SP V
Sbjct: 181 IIVARLRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKAILKSNAADSPGV 240

Query: 241 TVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300
           TVQNC ERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD
Sbjct: 241 TVQNCAERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSD 300

Query: 301 GTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAE 360
           GTPISSE ERQRVIQCLQAAIQRRASEGVRLELCT DRPGLLADV RTFRENGLNVTRAE
Sbjct: 301 GTPISSEPERQRVIQCLQAAIQRRASEGVRLELCTVDRPGLLADVMRTFRENGLNVTRAE 360

Query: 361 ISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALG 420
           IST  DMA N FYVTD +G+AADPK IESVRQRIGLS L VKE  S+  Q  EREEQ +G
Sbjct: 361 ISTARDMARNVFYVTDAVGNAADPKTIESVRQRIGLSELTVKEVASSCDQATEREEQTVG 420

Query: 421 VGGAVLFTLGSMVRRNLYNLGLIKA 446
           VGGAVLFTLGSMVRRNLYNLGLIK+
Sbjct: 421 VGGAVLFTLGSMVRRNLYNLGLIKS 445

BLAST of Lag0017609 vs. TAIR 10
Match: AT1G12420.1 (ACT domain repeat 8 )

HSP 1 Score: 568.9 bits (1465), Expect = 3.5e-162
Identity = 300/437 (68.65%), Postives = 351/437 (80.32%), Query Frame = 0

Query: 11  LDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVS 70
           LDEYEKLVIRMNTPRVVIDNG CS+AT+VKVDS+RR G LLEAVQ+LTDLNLSIKKAY+S
Sbjct: 7   LDEYEKLVIRMNTPRVVIDNGVCSSATIVKVDSSRRNGILLEAVQILTDLNLSIKKAYIS 66

Query: 71  SDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTALELTGTDRVG 130
           SDG W MDVFHVTD NG KL D+SV+ Y+EQS+ T +Y  +   NG  TALELTGTDR+G
Sbjct: 67  SDGTWNMDVFHVTDLNGNKLNDQSVLRYIEQSIETVYYGENIEVNG-LTALELTGTDRIG 126

Query: 131 LLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDIIIARLRSVL 190
           LLSE+FAVL+DL CDVV+AK+WTHNGR+AS+IY+KDC SG PI D  +I  I  RL++VL
Sbjct: 127 LLSEMFAVLSDLNCDVVDAKLWTHNGRVASVIYLKDCISGAPILDSHRISKIEGRLKNVL 186

Query: 191 KGDNDIRS-AKTSVSM-AVTHTERRLHQMMFADRDYERKPILKSNADSSPAVTVQNCTER 250
            GDND+ S AKT V++ ++ H ERRLHQ+MF DRDYER+   K        VTVQN  ER
Sbjct: 187 NGDNDVNSAAKTCVTVDSMMHIERRLHQLMFEDRDYERRS-KKHERSPMVVVTVQNWAER 246

Query: 251 GYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSDGTPISSEA 310
           GYSVV V C+DRTKLLFDV+ TLTDMEY VFHATINTAE++A+LEFYIRH DG+PISSEA
Sbjct: 247 GYSVVNVHCRDRTKLLFDVVCTLTDMEYAVFHATINTAEDQAHLEFYIRHKDGSPISSEA 306

Query: 311 ERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAEISTTGDMA 370
           ERQRVIQCL+AA++RRA EGVRLEL   D+ GLLA+VTRTFRENGLNVTR EIST+ DMA
Sbjct: 307 ERQRVIQCLEAAVERRALEGVRLELRHPDKQGLLAEVTRTFRENGLNVTRTEISTSSDMA 366

Query: 371 LNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQALGVGGAVLFT 430
            N FYVTD  G   D K+IESVR++IGL  L VKE P+ YH+  + EEQ      AVL +
Sbjct: 367 TNIFYVTDANGDEPDFKLIESVREKIGLECLRVKEMPTMYHKKGDGEEQQ--QTKAVLVS 426

Query: 431 LGSMVRRNLYNLGLIKA 446
           LGS+V RNL+N GLIK+
Sbjct: 427 LGSLVWRNLFNFGLIKS 439

BLAST of Lag0017609 vs. TAIR 10
Match: AT4G22780.1 (ACT domain repeat 7 )

HSP 1 Score: 543.5 bits (1399), Expect = 1.6e-154
Identity = 284/452 (62.83%), Postives = 352/452 (77.88%), Query Frame = 0

Query: 5   MEWPSCLDEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSI 64
           ME   C +EYEKLV+RMN PRVVIDNG C  +T+VK+DSAR  G LLE+VQ+LTD+NL I
Sbjct: 1   MELSDCSNEYEKLVVRMNMPRVVIDNGVCPNSTVVKIDSARSPGILLESVQLLTDMNLWI 60

Query: 65  KKAYVSSDGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGTTHYRRSEGFNGATTALELT 124
           KKAY+SSDG+W MDVFHV+D NG+KLTDE++I Y+E+S+ T+HY ++EG+ G  TALELT
Sbjct: 61  KKAYISSDGKWNMDVFHVSDLNGDKLTDENLIRYIEKSIETSHYCKTEGYTG-LTALELT 120

Query: 125 GTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIE-DRQKIDIII 184
           GTDRVGLLSEVFAVLADL+CDVVEAK WTHNGRIAS+IYVKD NSG PI+ D  ++  + 
Sbjct: 121 GTDRVGLLSEVFAVLADLECDVVEAKAWTHNGRIASMIYVKDGNSGTPIDGDSDRVQRVE 180

Query: 185 ARLRSVLKGDNDIRS-AKTSVSM-AVTHTERRLHQMMFADRDYERKPILKSNADSSPAVT 244
            +LR++LK D+  ++  +T VS    TH ERRLHQ MF DRDYE+    K + + SP V+
Sbjct: 181 GQLRNLLKADDGYQNDTRTCVSYGGNTHMERRLHQRMFMDRDYEK----KFDIEKSPIVS 240

Query: 245 VQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRHSDG 304
           VQN  +RGYSVV +QCKDR KLLFDV+ TLTDM Y+VFHA I T  E A+LEFY+RHSDG
Sbjct: 241 VQNLPKRGYSVVNLQCKDRMKLLFDVVCTLTDMAYIVFHAAIRTVGETAFLEFYVRHSDG 300

Query: 305 TPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTRAEI 364
            P+SSE ERQR+IQCLQAAI+RR  +GVRLELCTADRPGLLA+VTR  RENGLN+ RAEI
Sbjct: 301 HPVSSEPERQRLIQCLQAAIERRTVKGVRLELCTADRPGLLAEVTRILRENGLNIARAEI 360

Query: 365 STTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEA-PSTYHQTMEREE---- 424
           ST   +A N FYVTD  G+  DP+II+S+R++IG+ +L VKE  P +  + +E+E+    
Sbjct: 361 STKDSIARNVFYVTDANGNLIDPEIIKSIREKIGIDDLSVKEPFPISCREAVEKEQHIEP 420

Query: 425 ---QALGVGGAVLFTLGSMVRRNLYNLGLIKA 446
              Q    GG VL +LGS+V RNLY+LGLIK+
Sbjct: 421 QDHQGRYGGGTVLVSLGSLVMRNLYHLGLIKS 447

BLAST of Lag0017609 vs. TAIR 10
Match: AT1G69040.1 (ACT domain repeat 4 )

HSP 1 Score: 442.6 bits (1137), Expect = 3.8e-124
Identity = 237/448 (52.90%), Postives = 311/448 (69.42%), Query Frame = 0

Query: 12  DEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVSS 71
           +EYEKL+ RMN PRVVIDN +C  AT+++VDSA  YG LLE VQ+LTDLNL+I KAY+SS
Sbjct: 9   NEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTITKAYISS 68

Query: 72  DGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGT----THYRRSEGFNGAT--TALELTG 131
           DG WFMDVF+VTDQ+G K+TDE V+ Y+++SLG     +   RS G   +T  T +ELTG
Sbjct: 69  DGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDSTVIELTG 128

Query: 132 TDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDIIIAR 191
            DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +GC I D +++  I   
Sbjct: 129 CDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPERLSRIKNL 188

Query: 192 LRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSS-------P 251
           LR+VLKG N  R AKT VS    HT+RRLHQMMF DRDYE + +   + DSS       P
Sbjct: 189 LRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLV---DDDSSIQDERQRP 248

Query: 252 AVTVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRH 311
            V V N  ++ YSVV V+CKDR KLLFD + TLTDM+YVVFH +++T    A+ E+Y+RH
Sbjct: 249 DVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEYYVRH 308

Query: 312 SDGTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTR 371
            DG+P+ SEAE+QRVIQCL+AAI+RR SEG++LELCT DR GLL++VTR FREN L VTR
Sbjct: 309 IDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSLTVTR 368

Query: 372 AEISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQA 431
           AE+ T G  ALN FYV+D  G + D K I+S+RQ IG + L VK  P    Q  ++ +++
Sbjct: 369 AEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNP----QEQQQRQKS 428

Query: 432 LGVGGAVLFTLGSMVR-RNLYNLGLIKA 446
                   F  G + + ++  N GL+++
Sbjct: 429 PSHESPTRFLFGGLFKSKSFVNFGLVRS 449

BLAST of Lag0017609 vs. TAIR 10
Match: AT1G69040.2 (ACT domain repeat 4 )

HSP 1 Score: 442.6 bits (1137), Expect = 3.8e-124
Identity = 237/448 (52.90%), Postives = 311/448 (69.42%), Query Frame = 0

Query: 12  DEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVSS 71
           +EYEKL+ RMN PRVVIDN +C  AT+++VDSA  YG LLE VQ+LTDLNL+I KAY+SS
Sbjct: 13  NEYEKLIRRMNPPRVVIDNDSCKKATVIRVDSANEYGILLEVVQILTDLNLTITKAYISS 72

Query: 72  DGRWFMDVFHVTDQNGEKLTDESVISYLEQSLGT----THYRRSEGFNGAT--TALELTG 131
           DG WFMDVF+VTDQ+G K+TDE V+ Y+++SLG     +   RS G   +T  T +ELTG
Sbjct: 73  DGGWFMDVFNVTDQDGNKVTDEVVLDYIQKSLGPEACFSTSMRSVGVIPSTDSTVIELTG 132

Query: 132 TDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDIIIAR 191
            DR GLLSE+ AVL  L+C V+ A++WTHN R A+++ V D  +GC I D +++  I   
Sbjct: 133 CDRPGLLSELSAVLTHLKCSVLNAEIWTHNTRAAAVMQVTDDLTGCGISDPERLSRIKNL 192

Query: 192 LRSVLKGDNDIRSAKTSVSMAVTHTERRLHQMMFADRDYERKPILKSNADSS-------P 251
           LR+VLKG N  R AKT VS    HT+RRLHQMMF DRDYE + +   + DSS       P
Sbjct: 193 LRNVLKGSNTPREAKTVVSHGEVHTDRRLHQMMFEDRDYEHRLV---DDDSSIQDERQRP 252

Query: 252 AVTVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERAYLEFYIRH 311
            V V N  ++ YSVV V+CKDR KLLFD + TLTDM+YVVFH +++T    A+ E+Y+RH
Sbjct: 253 DVCVDNWLDKDYSVVTVRCKDRPKLLFDTVCTLTDMQYVVFHGSVDTEGTEAFQEYYVRH 312

Query: 312 SDGTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFRENGLNVTR 371
            DG+P+ SEAE+QRVIQCL+AAI+RR SEG++LELCT DR GLL++VTR FREN L VTR
Sbjct: 313 IDGSPVKSEAEKQRVIQCLEAAIKRRVSEGLKLELCTTDRVGLLSNVTRIFRENSLTVTR 372

Query: 372 AEISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQTMEREEQA 431
           AE+ T G  ALN FYV+D  G + D K I+S+RQ IG + L VK  P    Q  ++ +++
Sbjct: 373 AEVKTKGGKALNTFYVSDASGYSIDAKTIDSIRQTIGQTILKVKNNP----QEQQQRQKS 432

Query: 432 LGVGGAVLFTLGSMVR-RNLYNLGLIKA 446
                   F  G + + ++  N GL+++
Sbjct: 433 PSHESPTRFLFGGLFKSKSFVNFGLVRS 453

BLAST of Lag0017609 vs. TAIR 10
Match: AT2G03730.1 (ACT domain repeat 5 )

HSP 1 Score: 416.0 bits (1068), Expect = 3.8e-116
Identity = 232/455 (50.99%), Postives = 299/455 (65.71%), Query Frame = 0

Query: 12  DEYEKLVIRMNTPRVVIDNGACSTATLVKVDSARRYGNLLEAVQVLTDLNLSIKKAYVSS 71
           DE  K + R+N PRVVIDN  C   T++KVDSA ++G LLE VQVLT+LNL+IKKAY+SS
Sbjct: 13  DEIAKFIRRVNPPRVVIDNEVCKDVTVIKVDSANKHGILLEVVQVLTELNLTIKKAYISS 72

Query: 72  DGRWFMDVFHVTDQNGEKLTDESVISYLEQSLG-------TTHYRRSEGFNGAT--TALE 131
           DG WFMDVF+VTDQ+G K+TDE V+ Y+ +SLG       +   R + G   +   T +E
Sbjct: 73  DGGWFMDVFNVTDQDGNKVTDEIVLEYIRKSLGPDESSCFSPSMRSTIGVKQSVDYTVVE 132

Query: 132 LTGTDRVGLLSEVFAVLADLQCDVVEAKVWTHNGRIASLIYVKDCNSGCPIEDRQKIDII 191
           LTGTDR GLLSE+ AVL DLQC+VV A++WTH  + A+++ V D  +   I D +++  I
Sbjct: 133 LTGTDRPGLLSELCAVLMDLQCNVVNAEIWTHRAKAAAVLQVTDEETCSAITDPERLSKI 192

Query: 192 IARLRSVLKGDND---IRSAKTSVSMAV--THTERRLHQMMFADRDYERKPILKSNADSS 251
              L  VL G +     R  KT+VS A+  THT+R+LHQ+MFADRDY+     ++N D  
Sbjct: 193 RKLLGYVLTGGSSGRRFREPKTTVSSALNETHTDRKLHQLMFADRDYDE---WENNVDDE 252

Query: 252 -------PAVTVQNCTERGYSVVYVQCKDRTKLLFDVIFTLTDMEYVVFHATINTAEERA 311
                  P V V N  +  YS+V ++CKDR KLLFD +FTLTDM YVV HA+I+    +A
Sbjct: 253 DKCGRVIPDVDVSNLHDLDYSIVMIKCKDRPKLLFDTVFTLTDMNYVVSHASIDAEGPQA 312

Query: 312 YLEFYIRHSDGTPISSEAERQRVIQCLQAAIQRRASEGVRLELCTADRPGLLADVTRTFR 371
           Y E+YIRH+DG+P+ SEAERQRVI+CL+AAIQRR SEG++LELCT+DR GLL+DVTR FR
Sbjct: 313 YQEYYIRHTDGSPVKSEAERQRVIKCLKAAIQRRVSEGLKLELCTSDRVGLLSDVTRIFR 372

Query: 372 ENGLNVTRAEISTTGDMALNAFYVTDVIGSAADPKIIESVRQRIGLSNLIVKEAPSTYHQ 431
           EN L VTRAE+ T GD ALN FYV D  G   D K IES+RQ IG + L VK   +    
Sbjct: 373 ENSLTVTRAEVKTKGDKALNTFYVRDASGYQVDTKTIESIRQVIGQTILQVKGGNTDAKP 432

Query: 432 TMEREEQALGVGGAVLFTLGSMVRRNLYNLGLIKA 446
           + +             F  G    R+  N GLI++
Sbjct: 433 SPQDSPTG--------FLFGVFKSRSFVNFGLIRS 456