Lag0017258 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0017258
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	vacuolar protein sorting-associated protein 25
Location	chr5: 1482242 .. 1484836 (+)
RNA-Seq Expression	Lag0017258
Synteny	Lag0017258

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCAGAAATTGGGGGATTTCAAGTTACCACATTTCTTCAATTATCCACCATATTTCACGTAATTTTCCTACTCTGTCTTAGTTACCATTGTTTTACGTCATTATAAGCTACAACAAGTAACTTGGAAGTTGTCTTCGTCAATTTCTGTTGATAATGATATCTTGGTTAGAATGGCTGTTTTATGAGTGAATGAATGAAACTTTCGCTTTACTTTTCCCATAAGCCTCATAAATATGAACGTAATAATTCTATCTTGCTGCAATACCTGCAATTGTTATTGATAGCTTTGGTTGTTTCACTTTTTGCAAGCTTTTCTTTTGTCTTGAGTTGCATATATAACCTTTTATTGCATGTCTTCTCAGTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAGCTCTGGAAGGAACTAATTCTCGACTATTGCCGAACACAGAAGATATTTGTCATTGGACTCGAAGAGGAGTTTCCTCTATTTGCAAATTCTGTCATTGAGAGTAAATTCATGAACTTGTTTGGTCCTAATTGGTTGTTAAAACCTATTGATACATGCATAAATATCAACCTTAGAAAGGCCATTGACATTAACATCATGAACAGTGTGAAAAAGATTAAGGCCTATCATTTTTTGCAACCTTTTCACTCTACGAAGCATAGCATATCATTGGGATTGGTGTTTAGGACTCCAGTTTTTTTATGTATTTTACAGTGTGGTTGTGATGCTACAGGATCTCTTAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTTAGGCCCTGCTAGTTTAGTTCTATAATTCTATTTGTTTACAGTTTCCATTCATTCCCGAGGGAAAAACTGATATTTGGTACTTTTATTGTCCAATAGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTCATTTTATGGCACCGGATTCAAGATTGGGCCGACATCATTTTGCACTTTGTAAGACTAAATACTTGTTAACTTCATAGATATGTAAAAGTTGATTGGTTTTGGGTGGGTTTGGAAGGACTTTTGAAATGGTTAAAGTTGCTATTATCCCATTAAAAGCAATTTTCAAAATTCATATAATATTTATTTAGATAATCGAAGGTGCTTTGAAAAATTCTTATTCTAAAACTATTGCCTTGTTGAATAGGTGAAAAATAATGGACTTGAAGATAGTGTCATGACAGTCGAGGAAATACGTTCGGGAGTTGAATCTAGAGGAACAGGTACTTCCCTTGCTTCTTCTGAGTTGATTTAGATCTTCGAACGATTTCTAAATTCATTTTTTCATATGCTACATTTTTTAAGTACAACAAAGGTGTGGTAGGGAGATTTGAACCTCTAACCTCAAGGAAAGAAGTATATATCAATTACGGTCGAGTTATGCTCATATTGACTTTCATATGCTACATTGATATTGGTTTTAAAGCGACTCTAGTAGGTTAATTATTAATTAAATTAGGTGGACGTAAAAGCATCTTGGCGCTAAATAAATTCAGGAAATATGTAGATTGGTTTAAAGGAAAATGAAACTCTGGCATAAACTGTTTATATGAATGTATATCTTTTTAGTTTAAATCTCATTTCGACTTTTAAACTTCCATACTTGTTCTTATATGTCAACTAGAATATAGTTCAACTGGTTAAGATATATATTATCAATCAAGAGGTTAAAGGTTTAAATCCTCCCATCTCACATGTTGAACTCAAAAGAAAGCTTGTTCTTATATTTTTTTTAAATTTTCTACTTTGAATTCTTTGGTCCTTATCAAATCTGTAGATAATATTTCGCAAATTTGATGCTAAACCGAATGCATGAGACTTGAACATGAATTTAATTTATGTGATGTCATGTACGAATTTTGTGGAATGCTCTAAAAAGTTAGATGCTAAAGACAAATAATTTTCTACGCAAAATTCATAAACCGAATTGGAACATTTGTCCAAGGACTAAAACAAATCGATGTAAGCATTCTTGGACCACATTTTCTTCTTGAATGATGATATGTGCTTGCTGCTTGCGTTTGGTAGTGATTTTGGAATGACAAAAATCAATTTTTTCATATCTTTTTAGTTATTTTGGACATGACAAAAGTGTTTTTAGTCATTTCAAAGTACCTATATGTTTGTGTTTAAATACTAGGTTAAAATACCATTTGGTCCCTATACGTTGAGTGTTATTCCATATTAGTTCCTATACTTTCAAATGTCCAATTTTAGTCACTGTACTTTCAATAAAATTTAAAATTAGTTCCTACCATAAGTTTATTATTGTTTAACCGATTTTGAAATTTGACATTTGAGAGTATAGTGACTAAAATGAAACAAAACTCAAAGTACAGGGATCAAAATGATATTTAAACCTAAATACTACACTCTGCAATCACATCCAAACCCTATCTTCACGAACCAGTATATTTATTTTTCTTAAATGTCTGTTGTATGCATCAGAGCTTCAAGGGATTGATCGTACAATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGGAAGCTCGCAATTTTCAAGGGTTCTTCAACAGACGACGAAGGCATTAAATTCTCTGTATAA

mRNA sequence

ATGCAGAAATTGGGGGATTTCAAGTTACCACATTTCTTCAATTATCCACCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAGCTCTGGAAGGAACTAATTCTCGACTATTGCCGAACACAGAAGATATTTGTCATTGGACTCGAAGAGGAGTTTCCTCTATTTGCAAATTCTGTCATTGAGAGATCTCTTAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTCATTTTATGGCACCGGATTCAAGATTGGGCCGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTCATGACAGTCGAGGAAATACGTTCGGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACAATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGGAAGCTCGCAATTTTCAAGGGTTCTTCAACAGACGACGAAGGCATTAAATTCTCTGTATAA

Coding sequence (CDS)

ATGCAGAAATTGGGGGATTTCAAGTTACCACATTTCTTCAATTATCCACCATATTTCACTTTGCAACCAGTGAGGGACACCCGTGAGAAGCAAATTCAGCTCTGGAAGGAACTAATTCTCGACTATTGCCGAACACAGAAGATATTTGTCATTGGACTCGAAGAGGAGTTTCCTCTATTTGCAAATTCTGTCATTGAGAGATCTCTTAGTCATGAAGCTAGAGAAGCTTTTCTCTCAGCCTTAGTTTCAGATGGTCGTGCAGAATGGCTGGATAAGGGACATAGGAAATGCCTCATTTTATGGCACCGGATTCAAGATTGGGCCGACATCATTTTGCACTTTGTGAAAAATAATGGACTTGAAGATAGTGTCATGACAGTCGAGGAAATACGTTCGGGAGTTGAATCTAGAGGAACAGAGCTTCAAGGGATTGATCGTACAATTTTAATGAGAGCATTGAAGCAACTTGAACAGAAGGGGAAGCTCGCAATTTTCAAGGGTTCTTCAACAGACGACGAAGGCATTAAATTCTCTGTATAA

Protein sequence

MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLFANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV

Homology

[Close]

BLAST of Lag0017258 vs. NCBI nr
Match: XP_022949655.1 (vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_023005573.1 vacuolar protein sorting-associated protein 25 [Cucurbita maxima] >KAG6596709.1 Vacuolar protein sorting-associated protein 25, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 360.1 bits (923), Expect = 1.1e-95
Identity = 176/179 (98.32%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lag0017258 vs. NCBI nr
Match: XP_008448627.1 (PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo] >KAA0053011.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa] >TYK11467.1 vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 359.0 bits (920), Expect = 2.4e-95
Identity = 175/179 (97.77%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR+AFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELQGIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lag0017258 vs. NCBI nr
Match: XP_023540532.1 (vacuolar protein sorting-associated protein 25 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 358.6 bits (919), Expect = 3.1e-95
Identity = 175/179 (97.77%), Postives = 177/179 (98.88%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPV+DTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVKDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR  FLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARVVFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lag0017258 vs. NCBI nr
Match: XP_004146105.1 (vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hypothetical protein Csa_009640 [Cucumis sativus])

HSP 1 Score: 357.1 bits (915), Expect = 9.1e-95
Identity = 173/179 (96.65%), Postives = 178/179 (99.44%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NSVIERSLSHEAR+AF+SALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSVIERSLSHEARQAFISALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSGVESRGTEL+GIDRTILMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTVEEIRSGVESRGTELEGIDRTILMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lag0017258 vs. NCBI nr
Match: XP_038891553.1 (vacuolar protein sorting-associated protein 25 [Benincasa hispida])

HSP 1 Score: 357.1 bits (915), Expect = 9.1e-95
Identity = 173/179 (96.65%), Postives = 177/179 (98.88%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE+FPLF
Sbjct: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NS IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL
Sbjct: 61  SNSEIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMT+EEIRSGVESRGTELQGIDRT+LMRALK LEQKGKLAIFKGSSTDDEGIKFSV
Sbjct: 121 EDSVMTIEEIRSGVESRGTELQGIDRTVLMRALKLLEQKGKLAIFKGSSTDDEGIKFSV 179

[Close]

BLAST of Lag0017258 vs. ExPASy Swiss-Prot
Match: Q8VZC9 (Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 GN=VPS25 PE=1 SV=1)

HSP 1 Score: 324.7 bits (831), Expect = 6.5e-88
Identity = 153/179 (85.47%), Postives = 168/179 (93.85%), Query Frame = 0


Query: 1   MQKLGDFKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFPLF 60
           MQKL DFKLP FFNYPPYFTLQPVRDTREKQIQLWKELILDYC++QKIF+IG+EE+FPLF
Sbjct: 1   MQKLADFKLPQFFNYPPYFTLQPVRDTREKQIQLWKELILDYCKSQKIFLIGVEEDFPLF 60

Query: 61  ANSVIERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGL 120
           +NS I+RSLSHEARE FLSA+V +GRAEWLDKGHRKCLILWHRIQDWADI+L FV++NGL
Sbjct: 61  SNSAIDRSLSHEARETFLSAIVGEGRAEWLDKGHRKCLILWHRIQDWADIVLQFVRDNGL 120

Query: 121 EDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKFSV 180
           EDSVMTVEEIRSG ES GTELQGIDRTILMRALK LE KGKLA+FKG+S DDEG+KFSV
Sbjct: 121 EDSVMTVEEIRSGTESLGTELQGIDRTILMRALKLLENKGKLALFKGTSADDEGVKFSV 179

[Close]

BLAST of Lag0017258 vs. ExPASy Swiss-Prot
Match: Q6AX45 (Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps25 PE=2 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 9.3e-34
Identity = 70/173 (40.46%), Postives = 105/173 (60.69%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPLFANSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR  K++ + L E  E PLF N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLSAWSSLVLSYCRQNKLYTMNLMEIQESPLFNNKK 62

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           I+R LS E+ +  L  L   G  EW+DK   + LI+W R  +W  +I  +V  NG+ +SV
Sbjct: 63  IQRKLSLESVQVVLEELRKKGNLEWIDKNKSRFLIMWRRPDEWGKVIYQWVSKNGMTNSV 122

Query: 127 MTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++ G E  G+D  +L+R+L+ L+Q+ K  I   +  +  G+KF
Sbjct: 123 FTLYELISGDDTEGEEFHGLDEAMLLRSLEALQQEHKAEII--TLNESRGVKF 173

[Close]

BLAST of Lag0017258 vs. ExPASy Swiss-Prot
Match: Q6NWF4 (Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 PE=2 SV=2)

HSP 1 Score: 141.4 bits (355), Expect = 1.0e-32
Identity = 69/173 (39.88%), Postives = 106/173 (61.27%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEE--EFPLFANSV 66
           F+ P  +N+PP+FTLQP  DTR+KQ+  W  L+L YCR +K++ + + E  E P+F N  
Sbjct: 3   FEWPWQYNFPPFFTLQPNVDTRQKQLAAWCSLVLSYCRHRKLYTLDVLEAQESPVFNNKK 62

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           IER LS EA +     L   G  EWLDK   +CLI+W R ++W  +I  +V  NG+ +SV
Sbjct: 63  IERKLSVEAIQVVFEELRKKGNLEWLDKNKSRCLIMWRRPEEWGKLIYQWVSKNGMVNSV 122

Query: 127 MTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ +G ++   E  G++  +L+R+L+ L+  GK  I   +  D +G+KF
Sbjct: 123 FTLYELANGDDTEKEEFHGLEDWMLLRSLQALQTDGKAEII--TMDDGKGVKF 173

[Close]

BLAST of Lag0017258 vs. ExPASy Swiss-Prot
Match: Q55GD9 (Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44689 GN=vps25 PE=1 SV=1)

HSP 1 Score: 132.5 bits (332), Expect = 4.8e-30
Identity = 68/188 (36.17%), Postives = 114/188 (60.64%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCRTQKIFVIGLEEEFP----LFAN 66
           F+ P +++  P+FT+QP+ +TR+KQ Q+W++LIL YCR  KI+ + + E       LF N
Sbjct: 6   FQFPPYYHKEPFFTIQPILNTRKKQFQMWQDLILQYCRYYKIYELDINESIKSNSVLFNN 65

Query: 67  SVIERSLSHEAREAFLSALVSDGRAEWLDK-------------GHRKCLILWHRIQDWAD 126
             I R LS EA ++ +  ++ +G AEW+DK              + + LI+W +  +WA 
Sbjct: 66  EKINRKLSREALKSIIDDIIENGFAEWVDKEKEKEKEKDKDNNNNNRVLIMWRKPDEWAS 125

Query: 127 IILHFVKNNGLEDSVMTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSS 178
           +I  +V + GL ++V+TV EI++G +S+  E   ++ TILM++LK LE++ K   F  S 
Sbjct: 126 LIYKWVADCGLLNTVLTVWEIQNGDDSKKQEFHQLNTTILMKSLKVLEKQSKCQTF--SQ 185

[Close]

BLAST of Lag0017258 vs. ExPASy Swiss-Prot
Match: Q9CQ80 (Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25 PE=1 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 2.6e-28
Identity = 65/173 (37.57%), Postives = 98/173 (56.65%), Query Frame = 0


Query: 7   FKLPHFFNYPPYFTLQPVRDTREKQIQLWKELILDYCR--TQKIFVIGLEEEFPLFANSV 66
           F+ P  + +PP+FTLQP  DTR+KQ+  W  L+L +CR   Q    +   +E PLF N  
Sbjct: 5   FEWPWQYRFPPFFTLQPNVDTRQKQLAAWCSLVLSFCRLHKQSSMTVMEAQESPLFNNVK 64

Query: 67  IERSLSHEAREAFLSALVSDGRAEWLDKGHRKCLILWHRIQDWADIILHFVKNNGLEDSV 126
           ++R L  E+ +  L  L   G  EWLDK     LI+W R ++W  +I  +V  +G  +SV
Sbjct: 65  LQRKLPVESIQIVLEELRKKGNLEWLDKNKSSFLIMWRRPEEWGKLIYQWVSRSGQNNSV 124

Query: 127 MTVEEIRSGVESRGTELQGIDRTILMRALKQLEQKGKLAIFKGSSTDDEGIKF 178
            T+ E+ SG ++   E  G+D   L+RAL+ L+Q+ K  I   + +D  G+KF
Sbjct: 125 FTLYELTSGEDTEDEEFHGLDEATLLRALQALQQEHKAEII--TVSDGRGVKF 175

The following BLAST results are available for this feature:

BLAST of Lag0017258 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949655.1	1.1e-95	98.32	vacuolar protein sorting-associated protein 25 [Cucurbita moschata] >XP_02300557...	[more]
XP_008448627.1	2.4e-95	97.77	PREDICTED: vacuolar protein sorting-associated protein 25 isoform X1 [Cucumis me...	[more]
XP_023540532.1	3.1e-95	97.77	vacuolar protein sorting-associated protein 25 [Cucurbita pepo subsp. pepo]	[more]
XP_004146105.1	9.1e-95	96.65	vacuolar protein sorting-associated protein 25 [Cucumis sativus] >KGN55713.1 hyp...	[more]
XP_038891553.1	9.1e-95	96.65	vacuolar protein sorting-associated protein 25 [Benincasa hispida]	[more]

BLAST of Lag0017258 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8VZC9	6.5e-88	85.47	Vacuolar protein sorting-associated protein 25 OS=Arabidopsis thaliana OX=3702 G...	[more]
Q6AX45	9.3e-34	40.46	Vacuolar protein-sorting-associated protein 25 OS=Xenopus laevis OX=8355 GN=vps2...	[more]
Q6NWF4	1.0e-32	39.88	Vacuolar protein-sorting-associated protein 25 OS=Danio rerio OX=7955 GN=vps25 P...	[more]
Q55GD9	4.8e-30	36.17	Vacuolar protein-sorting-associated protein 25 OS=Dictyostelium discoideum OX=44...	[more]
Q9CQ80	2.6e-28	37.57	Vacuolar protein-sorting-associated protein 25 OS=Mus musculus OX=10090 GN=Vps25...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 103..178 e-value: 2.9E-25 score: 89.7
IPR014041	ESCRT-II complex, Vps25 subunit, N-terminal winged helix	GENE3D	1.10.10.570		coord: 5..101 e-value: 4.3E-31 score: 109.2
IPR008570	ESCRT-II complex, Vps25 subunit	PFAM	PF05871	ESCRT-II	coord: 13..145 e-value: 4.2E-43 score: 146.9
IPR008570	ESCRT-II complex, Vps25 subunit	PANTHER	PTHR13149	VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS25	coord: 1..179
None	No IPR available	PANTHER	PTHR13149:SF1	SUBFAMILY NOT NAMED	coord: 1..179
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 7..107
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 102..177

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0017258.1	Lag0017258.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0071985	multivesicular body sorting pathway
biological_process	GO:0043328	protein transport to vacuole involved in ubiquitin-dependent protein catabolic process via the multivesicular body sorting pathway
cellular_component	GO:0000814	ESCRT II complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0042803	protein homodimerization activity
molecular_function	GO:0005198	structural molecule activity