Lag0017148 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0017148
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionbasic endochitinase-like
Locationchr5: 573363 .. 576099 (+)
RNA-Seq ExpressionLag0017148
SyntenyLag0017148
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCACAGTTGTTTCGTAGCTCTGACCTTCGTTCTCTTGACGCAGTCGCCGTCGTCGGCGCAGCCGTGCGGCGGCGGTGGAGAATCGGCGGACGCCATGTGTCCGAGAGGCATCTGCAGCGCCGGGTCGGGGCTCTGCGGATCCGGATTCGTGAGGCGATGTGGTTCCGATATAACATTTGCGAGCCCTAACGGCGAAATTGGCAGCGTAATCAGCTCGTCGAATTTCGACGAAATGCTTCCGCATCGTGAGTCTTGCGAGAGCGGCGGATTTTACAGCTACCAGGCTTTTGTGGTTGCGGCGGCAGCGTTCGACGGCTTCGGCACCACTGGGAATCTCACCACCAGGAAGAGGGAGATCGCTGCTTTCCTCGCTCAAACATCTCACGAGACCTCAGGTTAATCCCTTGTACAAAGTTTCTTATCTACTTTATTCGATGTTTTAAAAAATAAGTAAACAATTGAAAACTAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAGAGAGAGAAGTTTTCAAAATTGTTTTTGTTTTTAGAATTTGGCGTAGGAATTCAAATGAATGTGATGTGAATTTAGAAAAAAAAAAACAAGCATAATTTTCAAAAACAAAAACCAAAAAACTAAGACCCTTCTGATAACCATTTGGTTTTTGGTTTTTGAAAATTATGCTTGTTTCTTCCCAATTTCCTTACAATGGTTTTCATCTTCCTCAAAGACACATTTGAATTCCTAACCAAATTCTAAAAACAAAAACAAGTTTTTGAAAACTATTTTTTTTTTAGTTTGAAAAATTTGACTTGGTTTTTGAAAACAAGGAAAAGATGTAGATAACAAAACAAAAAAACCTGTGGGTAAAAGTGGTGTTTGTAGGCTTAATTTTCAAAAACCAAAAAAGAAATGGTTATCAAACGGGCCTAAATGGTTATCAGGGCCTTTGTTTTCGAAACTTTGACTTGATTTCTAAAAACATTAGGATTTATAAATTAAATTAAAAAAAAAAACCAAATAGTTTTCAAATCAGACCTAAAATGTTTGATTTTCTCTATAGCTATTTCCTCTCGAAATTAATACATGTAATTACATTATGATGTGAAATCATAATTTTTAGTTTGGATAGAAAATAGTAAGCTACATAATCTCAAATAATTTCTAAGTAGATTTGGTCTATTTTTATGGTTGCATTATTTGGAAGATGATGTCATACCATAAGAATGTCATCGGTCTTTCTTATTGCTGTTTAATTTGAATAATTAATATTGATGACTAAATAGATGTGTAAAATATAAAAACTAAAATGATAGTAGCTAGAGTAGAAAAGAACATATTATATTACAACGTATAGGTGTAGTACAATTGTTTTTTTGTCCCACGACATTTAGTTATTTTGTTCGAAAGATCCTACACATTTAGATGTCAAGGGAATTTGTGGTATTTTGATGATTATTTCATCCAAATTTCTTTACTTGTTACCAATTCTAGACGTCCTACAAATAATATTAGAAAAACACACCTAAACATTAAGAATGGTATAAATTTTGGGAATCTCAATACAATTTAAATGGTTAGGATGTAGATAGATTGTAGGTTTAAATTCCGACTCTCGTGTGTAATGTAATATTAGGAAGAAATAGAAGATGAAATTTAAGAAAGGTACTGTTTGTAGGAGGACGAGGAACATGGCTTGATGGAGATACATGGGGCTATTGCTATATTAATTCAACCAACAAAGGTCCTTTTTATTGTAGTCCTTCTCCCAATTGGCCTTGTGCATCTGGCAAGGAATATTATGGCCGAGGTCCTTTCCAAATCACCTAGTAAGTTCTCACTTGTTACTTCTATCTTCCAATTATTACTTTTGTCATTCAACTATGATTTTTTTTTTTTTTTTATTGTTTTCAAAATTTGACTTGATTTTTTAAAACATGGGAAACAAGACAGATAACAAAATATAGAAACTTATGTGAAGATAGAGTTTGGAGGCTTAATTTTCAAAAACCAAAAACCAAATTGTTATTAAACAGGATTAAATGATTGTCAAACAAGATCTTAGTTTGTAGTTTTTCCTTTAGCTTTTGAAAATCTTAATTCTAAAATTACTTTTTTTTTAGCACATGATACAATGTAATTCATTTTCCATATGAAGCTCATAATAGCATACTCATTAATGTAAGAGAAAATAAATGTAAATTATCAAATTTTGTTAAATTTCATATTAGTAAAAAAAAAAAATGGTAAATATGAAAATGTTTACATGTAAGAGGTGACTTTTAAATGAAATAGTGCCGAAGAGAGTGAGCAGAATACTTTTCTTTTCAATTATGTTGATATATCCATTCGCATTATTGAAATTATCTTTGGATGACTTTTGGTTGATTAGTAACTTCAACTATGGGGCTGCTAGTAAAGCAATTGGGGTGGATCTGATAAACAATCCTGATCTAGTAGCTACCAATCCAACCATATCATTCAAGACAGCCATATGGTTCTGGATGCAGGGAAACAAACCATCGTGTCATGATGTTATGACCGATAAATGGATACCATCTAATGAAGATGTCTTGGCTAAGAGACTACCTGGCTATGGTCTAACCACCAACTTGATCAATATTAATGATTGTAGTAAAGAAAATGTTGATCAAGCAACCAGTAGGATTAGTTTCTACAAAAGGTATTGTGACATATTGGGCATCGAATATGGAGACAACTTGAATTGTACCGATCAAAAGCCTTTTATCTAG

mRNA sequence

ATGCACAGTTGTTTCGTAGCTCTGACCTTCGTTCTCTTGACGCAGTCGCCGTCGTCGGCGCAGCCGTGCGGCGGCGGTGGAGAATCGGCGGACGCCATGTGTCCGAGAGGCATCTGCAGCGCCGGGTCGGGGCTCTGCGGATCCGGATTCGTGAGGCGATGTGGTTCCGATATAACATTTGCGAGCCCTAACGGCGAAATTGGCAGCGTAATCAGCTCGTCGAATTTCGACGAAATGCTTCCGCATCGTGAGTCTTGCGAGAGCGGCGGATTTTACAGCTACCAGGCTTTTGTGGTTGCGGCGGCAGCGTTCGACGGCTTCGGCACCACTGGGAATCTCACCACCAGGAAGAGGGAGATCGCTGCTTTCCTCGCTCAAACATCTCACGAGACCTCAGGAGGACGAGGAACATGGCTTGATGGAGATACATGGGGCTATTGCTATATTAATTCAACCAACAAAGGTCCTTTTTATTGTAGTCCTTCTCCCAATTGGCCTTGTGCATCTGGCAAGGAATATTATGGCCGAGGTCCTTTCCAAATCACCTATAACTTCAACTATGGGGCTGCTAGTAAAGCAATTGGGGTGGATCTGATAAACAATCCTGATCTAGTAGCTACCAATCCAACCATATCATTCAAGACAGCCATATGGTTCTGGATGCAGGGAAACAAACCATCGTGTCATGATGTTATGACCGATAAATGGATACCATCTAATGAAGATGTCTTGGCTAAGAGACTACCTGGCTATGGTCTAACCACCAACTTGATCAATATTAATGATTGTAGTAAAGAAAATGTTGATCAAGCAACCAGTAGGATTAGTTTCTACAAAAGGTATTGTGACATATTGGGCATCGAATATGGAGACAACTTGAATTGTACCGATCAAAAGCCTTTTATCTAG

Coding sequence (CDS)

ATGCACAGTTGTTTCGTAGCTCTGACCTTCGTTCTCTTGACGCAGTCGCCGTCGTCGGCGCAGCCGTGCGGCGGCGGTGGAGAATCGGCGGACGCCATGTGTCCGAGAGGCATCTGCAGCGCCGGGTCGGGGCTCTGCGGATCCGGATTCGTGAGGCGATGTGGTTCCGATATAACATTTGCGAGCCCTAACGGCGAAATTGGCAGCGTAATCAGCTCGTCGAATTTCGACGAAATGCTTCCGCATCGTGAGTCTTGCGAGAGCGGCGGATTTTACAGCTACCAGGCTTTTGTGGTTGCGGCGGCAGCGTTCGACGGCTTCGGCACCACTGGGAATCTCACCACCAGGAAGAGGGAGATCGCTGCTTTCCTCGCTCAAACATCTCACGAGACCTCAGGAGGACGAGGAACATGGCTTGATGGAGATACATGGGGCTATTGCTATATTAATTCAACCAACAAAGGTCCTTTTTATTGTAGTCCTTCTCCCAATTGGCCTTGTGCATCTGGCAAGGAATATTATGGCCGAGGTCCTTTCCAAATCACCTATAACTTCAACTATGGGGCTGCTAGTAAAGCAATTGGGGTGGATCTGATAAACAATCCTGATCTAGTAGCTACCAATCCAACCATATCATTCAAGACAGCCATATGGTTCTGGATGCAGGGAAACAAACCATCGTGTCATGATGTTATGACCGATAAATGGATACCATCTAATGAAGATGTCTTGGCTAAGAGACTACCTGGCTATGGTCTAACCACCAACTTGATCAATATTAATGATTGTAGTAAAGAAAATGTTGATCAAGCAACCAGTAGGATTAGTTTCTACAAAAGGTATTGTGACATATTGGGCATCGAATATGGAGACAACTTGAATTGTACCGATCAAAAGCCTTTTATCTAG

Protein sequence

MHSCFVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDITFASPNGEIGSVISSSNFDEMLPHRESCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKREIAAFLAQTSHETSGGRGTWLDGDTWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGRGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWMQGNKPSCHDVMTDKWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNLNCTDQKPFI
Homology
BLAST of Lag0017148 vs. NCBI nr
Match: XP_022923516.1 (basic endochitinase C-like [Cucurbita moschata])

HSP 1 Score: 476.9 bits (1226), Expect = 1.3e-130
Identity = 231/307 (75.24%), Postives = 254/307 (82.74%), Query Frame = 0

Query: 1   MHSCFVALTFVLLTQSPSSAQPC-GGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDIT 60
           M   FVAL FVL T + S AQPC GGGGE  +AMCP+G+CSAGSG C SGFVRRCGSD+T
Sbjct: 1   MRKIFVALAFVLFTSATSWAQPCGGGGGEPENAMCPKGLCSAGSGFCRSGFVRRCGSDLT 60

Query: 61  FASPNGEIGSVISSSNFDEMLPHR--ESCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRK 120
           F SP GEIGSVIS+S+FDEML HR   SC+S GFYSY+AF VAAAAFDGFGTTGNL  RK
Sbjct: 61  FGSPTGEIGSVISASDFDEMLKHRNDRSCKSNGFYSYEAFAVAAAAFDGFGTTGNLNIRK 120

Query: 121 REIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGR 180
           RE+AAFLAQTSHET+GG  T  DG   WGYCYINST K    CSPS NWPCASGK+YYGR
Sbjct: 121 REVAAFLAQTSHETTGGWQTAPDGPYAWGYCYINSTIKDA-SCSPSTNWPCASGKQYYGR 180

Query: 181 GPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTD 240
           GPFQITYNFNYGAA +AIGVDLINNPDLVATNPTISFKTA+WFWM  QGNKPSCHDVMT 
Sbjct: 181 GPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMTG 240

Query: 241 KWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNLN 300
           KW+PSNED+ AKRLPGYGLT NLIN+++C     D+A  RI FYKRYCDIL I+YGDNL+
Sbjct: 241 KWVPSNEDISAKRLPGYGLTINLINVSECVNGTDDRAADRIGFYKRYCDILNIQYGDNLD 300

Query: 301 CTDQKPF 302
           C  Q+PF
Sbjct: 301 CVGQQPF 306

BLAST of Lag0017148 vs. NCBI nr
Match: KAG6577805.1 (Endochitinase A2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 469.5 bits (1207), Expect = 2.1e-128
Identity = 227/308 (73.70%), Postives = 255/308 (82.79%), Query Frame = 0

Query: 1   MHSCFVALTFVLLTQSPSSAQPC--GGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDI 60
           ++  F+AL FVL T + S AQPC  GGGGE  +AMCP+G+CSAGSG C SGFV+RCGSD+
Sbjct: 4   IYPSFLALAFVLFTSATSWAQPCGGGGGGEPENAMCPKGLCSAGSGFCRSGFVQRCGSDL 63

Query: 61  TFASPNGEIGSVISSSNFDEMLPHR--ESCESGGFYSYQAFVVAAAAFDGFGTTGNLTTR 120
           TF SP GEIGSVIS+S+FDEML HR   SC+S GFYSY+AF VAAAAFDGFGTTGNL  R
Sbjct: 64  TFGSPIGEIGSVISASDFDEMLKHRNDRSCKSNGFYSYEAFAVAAAAFDGFGTTGNLNIR 123

Query: 121 KREIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYG 180
           KRE+AAFLAQTSHET+GG  T  DG   WGYCYI+ST K    CSPS NWPCASGK+YYG
Sbjct: 124 KREVAAFLAQTSHETTGGWPTAPDGPYAWGYCYIDSTIKDA-SCSPSTNWPCASGKQYYG 183

Query: 181 RGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMT 240
           RGPFQITYNFNYGAA +AIGVDLINNPDLVATNPTISFKTA+WFWM  QGNKPSCHDVMT
Sbjct: 184 RGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMT 243

Query: 241 DKWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNL 300
            KW+PSNED+ AKRLPGYGLT NLIN+++C     D+A  RI FYKRYCDIL I+YGDNL
Sbjct: 244 GKWVPSNEDISAKRLPGYGLTINLINVSECVNGTDDRAADRIGFYKRYCDILNIQYGDNL 303

Query: 301 NCTDQKPF 302
           +C  Q+PF
Sbjct: 304 DCVGQQPF 310

BLAST of Lag0017148 vs. NCBI nr
Match: XP_023553171.1 (basic endochitinase C-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 467.2 bits (1201), Expect = 1.0e-127
Identity = 227/307 (73.94%), Postives = 251/307 (81.76%), Query Frame = 0

Query: 1   MHSCFVALTFVLLTQSPSSAQPCGGGG-ESADAMCPRGICSAGSGLCGSGFVRRCGSDIT 60
           ++  FVAL FVL T + S AQPCGGGG E  +AMCP+G+CSAGSG C SGFVRRCGSD+T
Sbjct: 4   IYRSFVALAFVLFTLATSWAQPCGGGGREPENAMCPKGLCSAGSGFCRSGFVRRCGSDLT 63

Query: 61  FASPNGEIGSVISSSNFDEMLPHR--ESCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRK 120
           F  P GEIG VIS+S+FDEML HR   +C+S GFYSY+AF VAAAAFDGFGTTGNL TRK
Sbjct: 64  FEGPIGEIGGVISASDFDEMLKHRNDRNCKSNGFYSYEAFAVAAAAFDGFGTTGNLNTRK 123

Query: 121 REIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGR 180
           RE+AAFLAQTSHET GG  T  DG   WGYCYINST K    CSPS NWPCASGK+YYGR
Sbjct: 124 REVAAFLAQTSHETKGGWQTAPDGPYAWGYCYINSTIKDA-SCSPSTNWPCASGKQYYGR 183

Query: 181 GPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTD 240
           GPFQITYNFNYGAA +AIGVDLINNPDLVATNPTISFKTA+WFWM  QGNKPSCHDVM  
Sbjct: 184 GPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMAG 243

Query: 241 KWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNLN 300
           KW+PSNED+ AKRLPGYGLT NLIN ++C     D+A  RI FYKRYCDIL I+YGDNL+
Sbjct: 244 KWVPSNEDISAKRLPGYGLTINLINASECVDGTDDRAADRIGFYKRYCDILNIQYGDNLD 303

Query: 301 CTDQKPF 302
           C  Q+PF
Sbjct: 304 CVGQQPF 309

BLAST of Lag0017148 vs. NCBI nr
Match: XP_023007623.1 (basic endochitinase C-like [Cucurbita maxima])

HSP 1 Score: 464.2 bits (1193), Expect = 8.9e-127
Identity = 225/308 (73.05%), Postives = 255/308 (82.79%), Query Frame = 0

Query: 1   MHSCFVALTFVLLTQSPSSAQPC--GGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDI 60
           ++  FVALTFVL   + S AQPC  GGGGE  +AMCP+G+CSAGSG CGSGFVRRCGSD+
Sbjct: 4   IYRSFVALTFVLFASATSWAQPCGGGGGGEPENAMCPKGLCSAGSGFCGSGFVRRCGSDL 63

Query: 61  TFASPNGEIGSVISSSNFDEMLPHR--ESCESGGFYSYQAFVVAAAAFDGFGTTGNLTTR 120
           TF SP GEIGSVISSS+FDEML HR   +C+S GFYSY+AF VAA+AF GFGTTGNL  R
Sbjct: 64  TFGSPIGEIGSVISSSDFDEMLQHRNDRNCKSNGFYSYEAFAVAASAFVGFGTTGNLNIR 123

Query: 121 KREIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYG 180
           KRE+AAFLAQTSHET+GG  +  DG   WGYCYI+ST K    C+PSPNWPCASGK+YYG
Sbjct: 124 KREVAAFLAQTSHETTGGWASAPDGPYAWGYCYIDSTIKDA-SCTPSPNWPCASGKQYYG 183

Query: 181 RGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMT 240
           RGPFQITYNFNYGAA +AIGVDLINNPDLVATNPTISFKTA+WFWM  QGNKPSCHDVMT
Sbjct: 184 RGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMT 243

Query: 241 DKWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNL 300
             W+PS+EDV AKRLPGYGLT NLIN+++C     D+A +RI FYKRYCDIL I+YGDNL
Sbjct: 244 GNWVPSDEDVSAKRLPGYGLTINLINVSECVDGTDDRAANRIGFYKRYCDILNIQYGDNL 303

Query: 301 NCTDQKPF 302
           +   Q+PF
Sbjct: 304 DYVGQQPF 310

BLAST of Lag0017148 vs. NCBI nr
Match: KAA0052928.1 (basic endochitinase C [Cucumis melo var. makuwa] >TYK11385.1 basic endochitinase C [Cucumis melo var. makuwa])

HSP 1 Score: 463.8 bits (1192), Expect = 1.2e-126
Identity = 223/305 (73.11%), Postives = 250/305 (81.97%), Query Frame = 0

Query: 3   SCFVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDITFAS 62
           S F+ALTF+LLT + SSAQ CGGGGE ADAMCP+G+C AGSGLCGSGFVRRCGSD+TF +
Sbjct: 7   SSFIALTFLLLTPATSSAQSCGGGGEPADAMCPKGLCIAGSGLCGSGFVRRCGSDLTFGT 66

Query: 63  PNGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKREI 122
           PNG +G VIS+S F+ ML HR    CE  GFYSY+AFVVAAAAFDGFGTTGNL TRKRE+
Sbjct: 67  PNGGLGGVISASEFEGMLKHRNDGGCEGNGFYSYEAFVVAAAAFDGFGTTGNLNTRKREV 126

Query: 123 AAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGRGPF 182
           AAFLAQTS+ET+GG  T  DG   WGYCYIN+T K    C PSPNWPC+SGK YYGRGPF
Sbjct: 127 AAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKYEL-CVPSPNWPCSSGKLYYGRGPF 186

Query: 183 QITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTDKWI 242
           QITYN+NYG A +AIGVDL+NNPDLVATNPTISFKTAIWFWM   GN PSCH+VMT KW+
Sbjct: 187 QITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKWV 246

Query: 243 PSNEDVLAKRLPGYGLTTNLIN-INDCSKENVDQATSRISFYKRYCDILGIEYGDNLNCT 302
           PSNEDV AKRLPGYGLT NLIN  N+C K    +A  R+ FY+RYCD+L I+YG NLNC 
Sbjct: 247 PSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYGGNLNCV 306

BLAST of Lag0017148 vs. ExPASy Swiss-Prot
Match: P51613 (Basic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 329.3 bits (843), Expect = 4.5e-89
Identity = 167/304 (54.93%), Postives = 206/304 (67.76%), Query Frame = 0

Query: 5   FVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDI-TFASP 64
           F  L+ +L+     SA+ C  GG++   +CP G C +  G CG+     CGS   +  S 
Sbjct: 9   FCLLSLILV----GSAEQC--GGQAGGRVCPGGACCSKFGWCGN-TADYCGSGCQSQCSS 68

Query: 65  NGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKREIA 124
            G+IG +I+ S F++ML HR   SC   GFY+Y AF+ AA AF GFGTTG+ TTRKREIA
Sbjct: 69  TGDIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIAAAKAFPGFGTTGDTTTRKREIA 128

Query: 125 AFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGRGPFQ 184
           AFLAQTSHET+GG  +  DG   WGYCY+        YC PS  WPCA+G++YYGRGP Q
Sbjct: 129 AFLAQTSHETTGGWASAPDGPYAWGYCYLREQGSPGAYCVPSAQWPCAAGRKYYGRGPIQ 188

Query: 185 ITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTDKWIP 244
           I+YN+NYG A KAIGVDL+NNPDLVAT+  ISFKTA WFWM  Q  KPSCH+V+T  W P
Sbjct: 189 ISYNYNYGQAGKAIGVDLVNNPDLVATDAVISFKTAFWFWMTPQSPKPSCHNVITGGWTP 248

Query: 245 SNEDVLAKRLPGYGLTTNLININ-DCSKENVDQATSRISFYKRYCDILGIEYGDNLNCTD 302
           S  D  A RLPG+G+ TN+IN   +C K  V Q   RI FYKRYCDIL + YG+NL+C +
Sbjct: 249 SGADRSAGRLPGFGVITNIINGGVECGKGVVPQVQDRIGFYKRYCDILRVSYGNNLDCNN 305

BLAST of Lag0017148 vs. ExPASy Swiss-Prot
Match: P93680 (Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2)

HSP 1 Score: 320.1 bits (819), Expect = 2.7e-86
Identity = 160/307 (52.12%), Postives = 203/307 (66.12%), Query Frame = 0

Query: 8   LTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGS-------GFVRRCGSDITF 67
           L  V L    + A+ C  G ++  A+CP G+C +  G CGS           +CG     
Sbjct: 13  LLLVGLLAGEAFAEQC--GRQAGGALCPGGLCCSQFGWCGSTSDYCGPTCQSQCGG--VT 72

Query: 68  ASPNGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKR 127
            SP G + S+IS S F++ML HR   +C++ GFY+Y AF+ AA +F+GF + G+  TRKR
Sbjct: 73  PSPGGGVASLISQSVFNQMLKHRNDAACQAKGFYTYNAFIAAANSFNGFASVGDTATRKR 132

Query: 128 EIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGRG 187
           EIAAFLAQTSHET+GG  T  DG   WGYC++      P YC P+  WPCA GK+YYGRG
Sbjct: 133 EIAAFLAQTSHETTGGWATAPDGPYAWGYCFLKEQGNPPDYCVPTAQWPCAPGKKYYGRG 192

Query: 188 PFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTDK 247
           P QI+YN+NYG A +AIG DLINNPD VAT+P ISFKTA+WFWM  Q  KPSCH+V+T +
Sbjct: 193 PIQISYNYNYGPAGRAIGYDLINNPDAVATDPVISFKTALWFWMTPQSPKPSCHNVITGR 252

Query: 248 WIPSNEDVLAKRLPGYGLTTNLININ-DCSKENVDQATSRISFYKRYCDILGIEYGDNLN 302
           W PS  D  A RLPGYG+ TN+IN   +C K   D+   RI FYKRYCD+LG+ YG NL+
Sbjct: 253 WTPSAADRAAGRLPGYGVITNIINGGIECGKGFNDKVADRIGFYKRYCDLLGVSYGSNLD 312

BLAST of Lag0017148 vs. ExPASy Swiss-Prot
Match: P08252 (Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2)

HSP 1 Score: 310.5 bits (794), Expect = 2.2e-83
Identity = 158/313 (50.48%), Postives = 205/313 (65.50%), Query Frame = 0

Query: 7   ALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGS--------GFVRRCGSDI 66
           +L F LL  S +SA+ C  G ++  A CP G+C +  G CG+            +C    
Sbjct: 11  SLLFSLLLLS-ASAEQC--GSQAGGARCPSGLCCSKFGWCGNTNDYCGPGNCQSQCPGGP 70

Query: 67  TFASP----NGEIGSVISSSNFDEMLPHR--ESCESGGFYSYQAFVVAAAAFDGFGTTGN 126
           T   P     G++GS+ISSS FD+ML HR   +C+  GFYSY AF+ AA +F GFGT+G+
Sbjct: 71  TPTPPTPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGD 130

Query: 127 LTTRKREIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGK 186
            T RKREIAAF AQTSHET+GG  T  DG   WGYC++        YC+PS  WPCA G+
Sbjct: 131 TTARKREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGR 190

Query: 187 EYYGRGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCH 246
           +Y+GRGP QI++N+NYG   +AIGVDL+NNPDLVAT+P ISFK+A+WFWM  Q  KPSCH
Sbjct: 191 KYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFWMTPQSPKPSCH 250

Query: 247 DVMTDKWIPSNEDVLAKRLPGYGLTTNLININ-DCSKENVDQATSRISFYKRYCDILGIE 302
           DV+  +W PS  D  A RLPG+G+ TN+IN   +C +    +   RI FY+RYC ILG+ 
Sbjct: 251 DVIIGRWQPSAGDRAANRLPGFGVITNIINGGLECGRGTDSRVQDRIGFYRRYCSILGVS 310

BLAST of Lag0017148 vs. ExPASy Swiss-Prot
Match: P19171 (Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3)

HSP 1 Score: 310.5 bits (794), Expect = 2.2e-83
Identity = 155/312 (49.68%), Postives = 202/312 (64.74%), Query Frame = 0

Query: 5   FVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGS--------GFVRRCGS 64
           F+ L F LL  S SSA+ C  G ++  A+CP G+C +  G CG+        G   +C  
Sbjct: 19  FLFLIFSLLL-SLSSAEQC--GRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQCTP 78

Query: 65  DITFASPNGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLT 124
             T   P G++  +ISSS FD+ML HR   +C + GFY+Y AF+ AA +F GFGTTG+  
Sbjct: 79  GGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTA 138

Query: 125 TRKREIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEY 184
           TRK+E+AAF  QTSHET+GG  T  DG  +WGYC+    N    YC PS  WPCASGK Y
Sbjct: 139 TRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKRY 198

Query: 185 YGRGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDV 244
           YGRGP Q+++N+NYG   +AIGVDL+NNPDLVA +  I+FK AIWFWM  Q  KPSCH V
Sbjct: 199 YGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAV 258

Query: 245 MTDKWIPSNEDVLAKRLPGYGLTTNLININ-DCSKENVDQATSRISFYKRYCDILGIEYG 303
           +  +W PS+ D  A RLPGYG+ TN+IN   +C +    +   RI FY+RYC+I G+  G
Sbjct: 259 IAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNPG 318

BLAST of Lag0017148 vs. ExPASy Swiss-Prot
Match: P11955 (26 kDa endochitinase 1 OS=Hordeum vulgare OX=4513 PE=2 SV=4)

HSP 1 Score: 309.3 bits (791), Expect = 4.8e-83
Identity = 156/316 (49.37%), Postives = 200/316 (63.29%), Query Frame = 0

Query: 5   FVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGS---------GFVRRCG 64
           FV    V +  + + A+ C  G ++  A CP  +C +  G CGS              CG
Sbjct: 4   FVLFAVVAMAATMAVAEQC--GSQAGGATCPNCLCCSRFGWCGSTPYCGDGCQSQCSGCG 63

Query: 65  SDITFASP----NGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGT 124
              T  +P     G + S++S + FD ML HR   +C++ GFY+Y AFV AA+AF GFGT
Sbjct: 64  GGSTPVTPTPSGGGGVSSIVSRALFDRMLLHRNDGACQAKGFYTYDAFVAAASAFRGFGT 123

Query: 125 TGNLTTRKREIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCA 184
           TG   TRKRE+AAFLAQTSHET+GG  T  DG   WGYC+         YC+PS  WPCA
Sbjct: 124 TGGTDTRKREVAAFLAQTSHETTGGWATAPDGAFAWGYCFKQERGATSNYCTPSAQWPCA 183

Query: 185 SGKEYYGRGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKP 244
            GK YYGRGP Q+++N+NYG A +AIGVDL+ NPDLVAT+PT+SFKTA+WFWM  Q  KP
Sbjct: 184 PGKSYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAPKP 243

Query: 245 SCHDVMTDKWIPSNEDVLAKRLPGYGLTTNLININ-DCSKENVDQATSRISFYKRYCDIL 302
           S H V+T +W PS  D  A R+PG+G+ TN++N   +C      +   RI FYKRYCDIL
Sbjct: 244 SSHAVITGQWSPSGTDRAAGRVPGFGVITNIVNGGIECGHGQDSRVADRIGFYKRYCDIL 303

BLAST of Lag0017148 vs. ExPASy TrEMBL
Match: A0A6J1E705 (basic endochitinase C-like OS=Cucurbita moschata OX=3662 GN=LOC111431189 PE=4 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 6.4e-131
Identity = 231/307 (75.24%), Postives = 254/307 (82.74%), Query Frame = 0

Query: 1   MHSCFVALTFVLLTQSPSSAQPC-GGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDIT 60
           M   FVAL FVL T + S AQPC GGGGE  +AMCP+G+CSAGSG C SGFVRRCGSD+T
Sbjct: 1   MRKIFVALAFVLFTSATSWAQPCGGGGGEPENAMCPKGLCSAGSGFCRSGFVRRCGSDLT 60

Query: 61  FASPNGEIGSVISSSNFDEMLPHR--ESCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRK 120
           F SP GEIGSVIS+S+FDEML HR   SC+S GFYSY+AF VAAAAFDGFGTTGNL  RK
Sbjct: 61  FGSPTGEIGSVISASDFDEMLKHRNDRSCKSNGFYSYEAFAVAAAAFDGFGTTGNLNIRK 120

Query: 121 REIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGR 180
           RE+AAFLAQTSHET+GG  T  DG   WGYCYINST K    CSPS NWPCASGK+YYGR
Sbjct: 121 REVAAFLAQTSHETTGGWQTAPDGPYAWGYCYINSTIKDA-SCSPSTNWPCASGKQYYGR 180

Query: 181 GPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTD 240
           GPFQITYNFNYGAA +AIGVDLINNPDLVATNPTISFKTA+WFWM  QGNKPSCHDVMT 
Sbjct: 181 GPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMTG 240

Query: 241 KWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNLN 300
           KW+PSNED+ AKRLPGYGLT NLIN+++C     D+A  RI FYKRYCDIL I+YGDNL+
Sbjct: 241 KWVPSNEDISAKRLPGYGLTINLINVSECVNGTDDRAADRIGFYKRYCDILNIQYGDNLD 300

Query: 301 CTDQKPF 302
           C  Q+PF
Sbjct: 301 CVGQQPF 306

BLAST of Lag0017148 vs. ExPASy TrEMBL
Match: A0A6J1L846 (basic endochitinase C-like OS=Cucurbita maxima OX=3661 GN=LOC111500198 PE=4 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 4.3e-127
Identity = 225/308 (73.05%), Postives = 255/308 (82.79%), Query Frame = 0

Query: 1   MHSCFVALTFVLLTQSPSSAQPC--GGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDI 60
           ++  FVALTFVL   + S AQPC  GGGGE  +AMCP+G+CSAGSG CGSGFVRRCGSD+
Sbjct: 4   IYRSFVALTFVLFASATSWAQPCGGGGGGEPENAMCPKGLCSAGSGFCGSGFVRRCGSDL 63

Query: 61  TFASPNGEIGSVISSSNFDEMLPHR--ESCESGGFYSYQAFVVAAAAFDGFGTTGNLTTR 120
           TF SP GEIGSVISSS+FDEML HR   +C+S GFYSY+AF VAA+AF GFGTTGNL  R
Sbjct: 64  TFGSPIGEIGSVISSSDFDEMLQHRNDRNCKSNGFYSYEAFAVAASAFVGFGTTGNLNIR 123

Query: 121 KREIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYG 180
           KRE+AAFLAQTSHET+GG  +  DG   WGYCYI+ST K    C+PSPNWPCASGK+YYG
Sbjct: 124 KREVAAFLAQTSHETTGGWASAPDGPYAWGYCYIDSTIKDA-SCTPSPNWPCASGKQYYG 183

Query: 181 RGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMT 240
           RGPFQITYNFNYGAA +AIGVDLINNPDLVATNPTISFKTA+WFWM  QGNKPSCHDVMT
Sbjct: 184 RGPFQITYNFNYGAAGRAIGVDLINNPDLVATNPTISFKTALWFWMTPQGNKPSCHDVMT 243

Query: 241 DKWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNL 300
             W+PS+EDV AKRLPGYGLT NLIN+++C     D+A +RI FYKRYCDIL I+YGDNL
Sbjct: 244 GNWVPSDEDVSAKRLPGYGLTINLINVSECVDGTDDRAANRIGFYKRYCDILNIQYGDNL 303

Query: 301 NCTDQKPF 302
           +   Q+PF
Sbjct: 304 DYVGQQPF 310

BLAST of Lag0017148 vs. ExPASy TrEMBL
Match: A0A5A7UCL1 (Basic endochitinase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00370 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.6e-127
Identity = 223/305 (73.11%), Postives = 250/305 (81.97%), Query Frame = 0

Query: 3   SCFVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDITFAS 62
           S F+ALTF+LLT + SSAQ CGGGGE ADAMCP+G+C AGSGLCGSGFVRRCGSD+TF +
Sbjct: 7   SSFIALTFLLLTPATSSAQSCGGGGEPADAMCPKGLCIAGSGLCGSGFVRRCGSDLTFGT 66

Query: 63  PNGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKREI 122
           PNG +G VIS+S F+ ML HR    CE  GFYSY+AFVVAAAAFDGFGTTGNL TRKRE+
Sbjct: 67  PNGGLGGVISASEFEGMLKHRNDGGCEGNGFYSYEAFVVAAAAFDGFGTTGNLNTRKREV 126

Query: 123 AAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGRGPF 182
           AAFLAQTS+ET+GG  T  DG   WGYCYIN+T K    C PSPNWPC+SGK YYGRGPF
Sbjct: 127 AAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKYEL-CVPSPNWPCSSGKLYYGRGPF 186

Query: 183 QITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTDKWI 242
           QITYN+NYG A +AIGVDL+NNPDLVATNPTISFKTAIWFWM   GN PSCH+VMT KW+
Sbjct: 187 QITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKWV 246

Query: 243 PSNEDVLAKRLPGYGLTTNLIN-INDCSKENVDQATSRISFYKRYCDILGIEYGDNLNCT 302
           PSNEDV AKRLPGYGLT NLIN  N+C K    +A  R+ FY+RYCD+L I+YG NLNC 
Sbjct: 247 PSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYGGNLNCV 306

BLAST of Lag0017148 vs. ExPASy TrEMBL
Match: A0A1S3BKQ5 (basic endochitinase C OS=Cucumis melo OX=3656 GN=LOC103490661 PE=4 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 5.6e-127
Identity = 223/305 (73.11%), Postives = 250/305 (81.97%), Query Frame = 0

Query: 3   SCFVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDITFAS 62
           S F+ALTF+LLT + SSAQ CGGGGE ADAMCP+G+C AGSGLCGSGFVRRCGSD+TF +
Sbjct: 39  SSFIALTFLLLTPATSSAQSCGGGGEPADAMCPKGLCIAGSGLCGSGFVRRCGSDLTFGT 98

Query: 63  PNGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKREI 122
           PNG +G VIS+S F+ ML HR    CE  GFYSY+AFVVAAAAFDGFGTTGNL TRKRE+
Sbjct: 99  PNGGLGGVISASEFEGMLKHRNDGGCEGNGFYSYEAFVVAAAAFDGFGTTGNLNTRKREV 158

Query: 123 AAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGRGPF 182
           AAFLAQTS+ET+GG  T  DG   WGYCYIN+T K    C PSPNWPC+SGK YYGRGPF
Sbjct: 159 AAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKYEL-CVPSPNWPCSSGKLYYGRGPF 218

Query: 183 QITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTDKWI 242
           QITYN+NYG A +AIGVDL+NNPDLVATNPTISFKTAIWFWM   GN PSCH+VMT KW+
Sbjct: 219 QITYNYNYGPAGQAIGVDLLNNPDLVATNPTISFKTAIWFWMTSHGNIPSCHEVMTGKWV 278

Query: 243 PSNEDVLAKRLPGYGLTTNLIN-INDCSKENVDQATSRISFYKRYCDILGIEYGDNLNCT 302
           PSNEDV AKRLPGYGLT NLIN  N+C K    +A  R+ FY+RYCD+L I+YG NLNC 
Sbjct: 279 PSNEDVSAKRLPGYGLTINLINGNNECGKGADTRAADRLGFYQRYCDVLNIQYGGNLNCV 338

BLAST of Lag0017148 vs. ExPASy TrEMBL
Match: A0A0A0L646 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002420 PE=4 SV=1)

HSP 1 Score: 456.1 bits (1172), Expect = 1.2e-124
Identity = 221/308 (71.75%), Postives = 253/308 (82.14%), Query Frame = 0

Query: 1   MHSCFVALTFVLL-TQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGSGFVRRCGSDIT 60
           +++ F+ LTFVLL T + SSAQ C GGGESADAMCP+G+C AGSGLCGSGF+RRCGSD+T
Sbjct: 4   IYTSFITLTFVLLTTPATSSAQSC-GGGESADAMCPKGLCIAGSGLCGSGFLRRCGSDLT 63

Query: 61  FASPNGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRK 120
           F +PN  +G V+S+S FD ML HR   SCE  GFYSY+AFVVAAA FDGFGTTGNL+TRK
Sbjct: 64  FGNPNEGMGGVVSASEFDGMLKHRNDGSCEGNGFYSYEAFVVAAAEFDGFGTTGNLSTRK 123

Query: 121 REIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEYYGR 180
           RE+AAFLAQTS+ET+GG  T  DG   WGYCYIN+T K  F C PSP WPC+SGK YYGR
Sbjct: 124 REVAAFLAQTSYETTGGWATAPDGPYAWGYCYINATQKDQF-CVPSPTWPCSSGKLYYGR 183

Query: 181 GPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTD 240
           GPFQI YNFNYGAA KAIGVDL+NNPDLVATNPTISFKTA+WFWM  QG+KPSCHDVMT 
Sbjct: 184 GPFQIAYNFNYGAAGKAIGVDLLNNPDLVATNPTISFKTALWFWMTSQGDKPSCHDVMTG 243

Query: 241 KWIPSNEDVLAKRLPGYGLTTNLIN-INDCSKENVDQATSRISFYKRYCDILGIEYGDNL 300
           KW+PSNEDV AKRLPGYGLT N+IN  N+C K    +A  R+ FY+RYCD+L I+YGDN 
Sbjct: 244 KWVPSNEDVSAKRLPGYGLTINIINGNNECGKGADARAADRLGFYQRYCDVLNIQYGDNF 303

Query: 301 NCTDQKPF 302
           NC +QKPF
Sbjct: 304 NCVNQKPF 309

BLAST of Lag0017148 vs. TAIR 10
Match: AT3G12500.1 (basic chitinase )

HSP 1 Score: 310.5 bits (794), Expect = 1.5e-84
Identity = 155/312 (49.68%), Postives = 202/312 (64.74%), Query Frame = 0

Query: 5   FVALTFVLLTQSPSSAQPCGGGGESADAMCPRGICSAGSGLCGS--------GFVRRCGS 64
           F+ L F LL  S SSA+ C  G ++  A+CP G+C +  G CG+        G   +C  
Sbjct: 19  FLFLIFSLLL-SLSSAEQC--GRQAGGALCPNGLCCSEFGWCGNTEPYCKQPGCQSQCTP 78

Query: 65  DITFASPNGEIGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLT 124
             T   P G++  +ISSS FD+ML HR   +C + GFY+Y AF+ AA +F GFGTTG+  
Sbjct: 79  GGTPPGPTGDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTA 138

Query: 125 TRKREIAAFLAQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPSPNWPCASGKEY 184
           TRK+E+AAF  QTSHET+GG  T  DG  +WGYC+    N    YC PS  WPCASGK Y
Sbjct: 139 TRKKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKRY 198

Query: 185 YGRGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDV 244
           YGRGP Q+++N+NYG   +AIGVDL+NNPDLVA +  I+FK AIWFWM  Q  KPSCH V
Sbjct: 199 YGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAV 258

Query: 245 MTDKWIPSNEDVLAKRLPGYGLTTNLININ-DCSKENVDQATSRISFYKRYCDILGIEYG 303
           +  +W PS+ D  A RLPGYG+ TN+IN   +C +    +   RI FY+RYC+I G+  G
Sbjct: 259 IAGQWQPSDADRAAGRLPGYGVITNIINGGLECGRGQDGRVADRIGFYQRYCNIFGVNPG 318

BLAST of Lag0017148 vs. TAIR 10
Match: AT4G01700.1 (Chitinase family protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.5e-60
Identity = 109/242 (45.04%), Postives = 156/242 (64.46%), Query Frame = 0

Query: 67  IGSVISSSNFDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKREIAAFL 126
           I S++  + +D++  H++  +C + GFY Y+AFV A  +F  FG+ GN  TR+RE+AAFL
Sbjct: 38  IMSLVPRTLYDQIFIHKDNNACPAKGFYPYEAFVEATRSFPKFGSVGNFWTRRREVAAFL 97

Query: 127 AQTSHETSGGRGTWLDGD-TWGYCYINSTNKGPFYCSPS-PNWPCASGKEYYGRGPFQIT 186
           AQ SHET+GG  T  DG   WG C+    +    YC  S  +WPC SGK Y GRGP Q++
Sbjct: 98  AQISHETTGGWATAPDGPYAWGLCFKEEVSPQSNYCDASNKDWPCVSGKSYKGRGPIQLS 157

Query: 187 YNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTDKWIPSN 246
           +N+NYG A +A+G D + NP+LVA N  ++FKTA+WFWM  Q  KPSCH+VM +++ P+ 
Sbjct: 158 WNYNYGQAGRALGFDGLQNPELVANNSVLAFKTALWFWMTEQTPKPSCHNVMVNRYRPTK 217

Query: 247 EDVLAKRLPGYGLTTNLININ-DCSKENVDQATSRISFYKRYCDILGIEYGDNLNCTDQK 302
            D  A R  GYGL TN+IN   +C      + T R+ +++RY  +  +  G NL+C +Q+
Sbjct: 218 ADRAANRTVGYGLVTNIINGGLECGIPGDGRVTDRVGYFQRYAQLFKVTTGPNLDCENQR 277

BLAST of Lag0017148 vs. TAIR 10
Match: AT1G02360.1 (Chitinase family protein )

HSP 1 Score: 212.6 bits (540), Expect = 4.3e-55
Identity = 100/233 (42.92%), Postives = 146/233 (62.66%), Query Frame = 0

Query: 76  FDEMLPHRE--SCESGGFYSYQAFVVAAAAFDGFGTTGNLTTRKREIAAFLAQTSHETSG 135
           ++++  H++  +C + GFY+Y++FV A   F  FG+ G+  T++ E+AAFLAQ SHET+G
Sbjct: 39  YNKIFIHKDNTACPANGFYTYESFVQATRRFPRFGSVGSPVTQRLEVAAFLAQISHETTG 98

Query: 136 GRGTWLDGD-TWGYCYINSTNKGPFYC-SPSPNWPCASGKEYYGRGPFQITYNFNYGAAS 195
           G  T  DG   WG C+    +    YC S    WPC   K Y GRGP Q+++N+NYG A 
Sbjct: 99  GWATAPDGPYAWGLCFKEEVSPQSTYCDSSDTQWPCFPNKTYQGRGPIQLSWNYNYGPAG 158

Query: 196 KAIGVDLINNPDLVATNPTISFKTAIWFWM--QGNKPSCHDVMTDKWIPSNEDVLAKRLP 255
           +A+G D + NP+ V+ N  I+F+TA+WFWM  Q  KPSCHDVM  K+ P+  D+ A R  
Sbjct: 159 RALGFDGLRNPETVSNNSVIAFQTALWFWMTPQSPKPSCHDVMIGKYRPTAADLAANRTG 218

Query: 256 GYGLTTNLININ-DCSKENVDQATSRISFYKRYCDILGIEYGDNLNCTDQKPF 302
           G+GLTTN+IN   +C      +   RI F++RY  +  +  G NL+C +Q+P+
Sbjct: 219 GFGLTTNIINGGLECGIPGDGRVNDRIGFFQRYTGLFKVATGPNLDCENQRPY 271

BLAST of Lag0017148 vs. TAIR 10
Match: AT2G43620.1 (Chitinase family protein )

HSP 1 Score: 172.6 bits (436), Expect = 5.0e-43
Identity = 109/312 (34.94%), Postives = 156/312 (50.00%), Query Frame = 0

Query: 1   MHSCFVALTFVLLTQSPS-SAQPCGGGGESADAMCPR-GICSAGSGLCGSGF-VRRCGSD 60
           + + F+   F L   + +  +Q CG  G +A+  C R G C      CG+G     C S 
Sbjct: 8   LKNAFILFLFTLTIMAKTVFSQQCGTTGCAANLCCSRYGYCGTTDAYCGTGCRSGPCSSS 67

Query: 61  IT-------------FASPNGEIGSVISSSNFDEMLPH-RESCESGGFYSYQAFVVAAAA 120
            T              A P   I +V++ + FD ++      C + GFY+ QAF+ AA +
Sbjct: 68  TTPIPPTPSGGAGGLNADPRDTIENVVTPAFFDGIMSKVGNGCPAKGFYTRQAFIAAAQS 127

Query: 121 FDGFGTTGNLTTRKREIAAFLAQTSHETSGGRGTWLDGDTWGYCYINSTNKGPFYCSPSP 180
           FD +      T  KREIAA LAQ SHE+              +CY     +G  YCSPS 
Sbjct: 128 FDAY----KGTVAKREIAAMLAQFSHESG------------SFCYKEEIARGK-YCSPST 187

Query: 181 NWPCASGKEYYGRGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWMQG 240
            +PC  GK+YYGRGP QIT+N+NYGAA K +G+ L+ +PD+VA +P ++F+ A+WFW   
Sbjct: 188 AYPCTPGKDYYGRGPIQITWNYNYGAAGKFLGLPLLTDPDMVARSPQVAFQCAMWFWNLN 247

Query: 241 NKPSCHDVMTDKWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCD 296
            +P                VL +   G+G TT  IN  +C+        SR+++Y  +C 
Sbjct: 248 VRP----------------VLDQ---GFGATTRKINGGECNGRRPAAVQSRVNYYLEFCR 283

BLAST of Lag0017148 vs. TAIR 10
Match: AT2G43610.1 (Chitinase family protein )

HSP 1 Score: 171.8 bits (434), Expect = 8.5e-43
Identity = 109/301 (36.21%), Postives = 152/301 (50.50%), Query Frame = 0

Query: 9   TFVLLTQSPSSAQPCGGGGESADAMCPR-GICSAGSGLCGSG------------FVRRCG 68
           T  ++T +  S Q CG  G   +  C R G C      CG+G                 G
Sbjct: 18  TLTIMTGTAFS-QNCGTNGCKGNMCCSRWGYCGTTKAYCGTGCQSGPCNSKPKPTPTPSG 77

Query: 69  SDITFASPNGEIGSVISSSNFDEMLPHRES-CESGGFYSYQAFVVAAAAFDGFGTTGNLT 128
           S    A P G I SVI+ + F+ ++    S C + GFY+ QAF+ AA +F  +      T
Sbjct: 78  SGGLNAGPRGTIASVITPAFFNSIMSKVGSGCPAKGFYTRQAFIAAAESFAAY----KGT 137

Query: 129 TRKREIAAFLAQTSHETSGGRGTWLDGDTWGYCYINSTNKGPFYCSPSPNWPCASGKEYY 188
             KREIAA LAQ SHE+              +CY     +G  YCSPS  +PC  GK YY
Sbjct: 138 VAKREIAAMLAQFSHESG------------SFCYKEEIARGR-YCSPSTTYPCQPGKNYY 197

Query: 189 GRGPFQITYNFNYGAASKAIGVDLINNPDLVATNPTISFKTAIWFWMQGNKPSCHDVMTD 248
           GRGP QIT+N+NYGAA K +G+ L+ +PD+VA +PT++F+ A+WFW +  +P        
Sbjct: 198 GRGPIQITWNYNYGAAGKFLGLPLLKDPDMVARSPTVAFQCAMWFWNKNVRP-------- 257

Query: 249 KWIPSNEDVLAKRLPGYGLTTNLININDCSKENVDQATSRISFYKRYCDILGIEYGDNLN 296
                   VL++   G+G TT  IN  +C+        SR++ Y  +C  LG+  G NL+
Sbjct: 258 --------VLSQ---GFGATTRRINGGECNGGRPAAVQSRVNHYLDFCKKLGVTPGTNLS 281

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022923516.11.3e-13075.24basic endochitinase C-like [Cucurbita moschata][more]
KAG6577805.12.1e-12873.70Endochitinase A2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023553171.11.0e-12773.94basic endochitinase C-like [Cucurbita pepo subsp. pepo][more]
XP_023007623.18.9e-12773.05basic endochitinase C-like [Cucurbita maxima][more]
KAA0052928.11.2e-12673.11basic endochitinase C [Cucumis melo var. makuwa] >TYK11385.1 basic endochitinase... [more]
Match NameE-valueIdentityDescription
P516134.5e-8954.93Basic endochitinase OS=Vitis vinifera OX=29760 GN=CHIT1B PE=2 SV=1[more]
P936802.7e-8652.12Endochitinase OS=Persea americana OX=3435 GN=chi1 PE=1 SV=2[more]
P082522.2e-8350.48Endochitinase A OS=Nicotiana tabacum OX=4097 GN=CHN48 PE=1 SV=2[more]
P191712.2e-8349.68Basic endochitinase B OS=Arabidopsis thaliana OX=3702 GN=CHI-B PE=1 SV=3[more]
P119554.8e-8349.3726 kDa endochitinase 1 OS=Hordeum vulgare OX=4513 PE=2 SV=4[more]
Match NameE-valueIdentityDescription
A0A6J1E7056.4e-13175.24basic endochitinase C-like OS=Cucurbita moschata OX=3662 GN=LOC111431189 PE=4 SV... [more]
A0A6J1L8464.3e-12773.05basic endochitinase C-like OS=Cucurbita maxima OX=3661 GN=LOC111500198 PE=4 SV=1[more]
A0A5A7UCL15.6e-12773.11Basic endochitinase C OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13... [more]
A0A1S3BKQ55.6e-12773.11basic endochitinase C OS=Cucumis melo OX=3656 GN=LOC103490661 PE=4 SV=1[more]
A0A0A0L6461.2e-12471.75Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002420 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G12500.11.5e-8449.68basic chitinase [more]
AT4G01700.11.5e-6045.04Chitinase family protein [more]
AT1G02360.14.3e-5542.92Chitinase family protein [more]
AT2G43620.15.0e-4334.94Chitinase family protein [more]
AT2G43610.18.5e-4336.21Chitinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.530.10coord: 56..302
e-value: 6.0E-70
score: 237.1
NoneNo IPR availableGENE3D3.30.20.10Endochitinase, domain 2coord: 151..203
e-value: 5.3E-26
score: 92.2
NoneNo IPR availablePANTHERPTHR22595CHITINASE-RELATEDcoord: 5..301
NoneNo IPR availablePANTHERPTHR22595:SF171CHITINASE (CLASS IB) / HEVEINcoord: 5..301
NoneNo IPR availableCDDcd00325chitinase_GH19coord: 71..295
e-value: 6.39835E-92
score: 270.455
IPR016283Glycoside hydrolase, family 19PIRSFPIRSF001060Endochitinasecoord: 1..302
e-value: 1.8E-99
score: 330.9
IPR000726Glycoside hydrolase, family 19, catalyticPFAMPF00182Glyco_hydro_19coord: 70..295
e-value: 5.7E-96
score: 320.8
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00773CHITINASE_19_1coord: 86..108
IPR000726Glycoside hydrolase, family 19, catalyticPROSITEPS00774CHITINASE_19_2coord: 211..221
IPR023346Lysozyme-like domain superfamilySUPERFAMILY53955Lysozyme-likecoord: 67..301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0017148.1Lag0017148.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
biological_process GO:0016998 cell wall macromolecule catabolic process
biological_process GO:0006032 chitin catabolic process
biological_process GO:0050832 defense response to fungus
molecular_function GO:0004568 chitinase activity
molecular_function GO:0008061 chitin binding