Lag0016880 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016880
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectinesterase
Locationchr12: 42045189 .. 42049878 (+)
RNA-Seq ExpressionLag0016880
SyntenyLag0016880
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCACAGCCCGACGATTCCGCCGCCGCCGGAGTTCCCTTCTCCGGCAGCCCCAAAAAGAAGAACCTCTTCATCCTAGCCCTCCTCCTCGTCATCGGCACTGTCGCCGGCATCTCCTCCTCGGAAGACGACCACGTGACCGTCGTGAGAAGCACGTGCGCCGAGACGCTCTACCCGGAGCTGTGCCAGTCGACGATATCCGCCGCCGTGCGAGGTGGTTCGAAGAAGGTGTCGAGTTCGAAAGACGTAATCGAAGCGTCGGTGAACATCACGATCGGTGCAGTGAAGCGGAACTACAAAAGCATAAAGAAATTGGTTGCGACGAGGAAGAATCTGAGCAAGCGAGAGAAAATTGCTCTGGAGGATTGTCTGGAGAACGGCGAGGAGACGCTGAGAGAGCTGTATGAAGTGGTTGAAGATCTTAATGAGTATCCTAAGAAGAAGGCTCTGTCGCAGTACGCCGATGACCTCAAAACCTTGCTCAGTTCCGCCATTACTAATCAGGTACCTCATTTTTAATTTCTGGTTCTTAGATTTTTTTTTTTCATTTTTTGAGTTTTATCTTTGATTTTTTTTTTTTTTTTTAAGTATTCAAATTTGGAAGACTATATATTGCTTACCCAAAAAAAAAAAAAAAAAAAAAAAGGTTTTGGTTGTTGGATTTTTAAAATTTTTATTGGGATTTTTAGACCTTTAAAATCTTTATAACATTTTGATGGTGATATGTAAGGAGATCCGTGAATATTTTTCTTTTATTTTGGGTTTAAATTTTGAGAAACATATATTTTGGTGTTTTTTTTTAGTTTTAATAAAATGTTAAATTCACCAAACTAAACACGATTTACAAAAAAAAAAAAAAAAAAAAAAAAAATACAATATGTGGGTGGAGGAATTCAAACATTTGAGTTCTTAGTCGGAAAAACATGTGCTTTAATCAATTGAGAACTAGATGTAGGTTAGTGCACGAAACACTTTTTTTTTTTTTTTGACAATATGTAGGGGTGGGGGATTCGAACCATATACCTCGTGGTTATCAATACAACTTTATGTCAGTTGAGCTATGCTCTTGTTGGCACACGATACACCTATTACTTGATCAATGATTTGCCCTTTTGCCTCATTTCCTCCCACAATCTTGAAATAATAATAATAATAAAAAAAAAACATAAATGTGGAGGAAATGACTATATACCCCTCCAACTTCACAGAATATAAACATCGTCAAAGCTAACATAACTCAACAGTAATTGACATGTACTCTCGACCAAGCAGTCAGAGATTCGAATTTCCCCATCCCAAATGGTGATGAAATTAAAAAAAAAAAAAAACAAAATAAACATCTTAAAGTTATTTTTAAAATATATTTTAGTAAATGTGAGAGTAGAAATTCAAAATTGTGTTTACTTAGTAATAATTATGTTAGTTCTTTTTTTTTTTTTTTTTGAAACAATTATGTTAGTTCTTATGTCTACGTGGTTTCCTGTTTTATTTACTATATTTGTTTGACTTTGCTTTCTTTTGTTTTTTTCGTAATATATTACATTTGAAAAGAAAAATAATAAAGTAAGTTATTACAAACACACAGAAAAAAAAAAATATATATATATATATAAACTAAAATAGGATGTTCAAAAATATATATATATATAAAAACTAAAATAAGAAAATCTATAAAATTTAGGGGAAAAAAAAAGTTGAAAATAGACGTTTTCCTGCCCGGCCGGTCAAAATTTATGCAGCCCTTACGAACAGCCGTGAGTTCCAAAACAGATTCATTAAAAACTAACATCTTTTTACAACTCAGGAGACATGTCTGGACGGATTCTCGCACGACAAAGCCGACAAGATCGTGCGAAAGTCATTAGAATCCGGCCAAATCCACGTCGAGAAACTCTGCAGCATCGCTTTAGCATTGATCAAGAATCTCACCGACACCGACATTGCCAATCGGAACAACAACCTCGTCAGAAAGCAGCTGACGGAGGACGACGGAATTCAGTGGCCGGATTGGCTGCCTGCCGGTGACCGGAGGCTTTTGCAGGCGTCGTCGGCGGCGACTCCGGACGCGGTGGTTGCCGCCGACGGGAGCGGAGATTTCACGACGGTGTCGGCGGCGGTAGCGGCGGCGCCGAATGGGAGTAGCCGGAGATATATAATAAGAATTAAGGCGGGGGTTTATAGAGAGAATGTGAATGTGGCGAGTGGCAAGAGGAATATAATGTTTTGGGGAGATGGAAGAACCACTACTATTATCACCGGAAATAGAAATGTCGTCGACGGCAGCACCACCTTCAACTCCGCCACCGTTGGTATGATACTCTCAATTTACTTTTTTTTTTTTTTTTTTTTTTATCAATTTTAGTAGGTTAGACTTTTTAGATGTATGATAGTCCTTAAATTTTCAAGCTTATATCTAAAACAAATCTCTTGGTTTTTTTTTTTTTAAAGTGTCTAATATGTCATAATTTTGAGTCTAATGGGTTATTAACATAATATTTCTTATAAATTAAAATTCATTTTTTTATTATTGTTTTTTTTAAATAATTTTATTATTGTTTTTTCATGTTGGATAATTTTCTAAACTTTTAATTGTGTGTGTTTTTTAAAGTTGAATTATGTCAAAAGACAAAGACCTATTGGATAGAAAATTAAAAATTGAGAAACTTATTAGATGTAAAATTAAAAGTTTGAGAACCTTTTAAATACTGCGTAAAGTAAATGATCCAAATAGACACAAAGTTGTCCATAAGAGCATAGCTCAACTGGTATAAAGTTATACTGATGACTACGATATATGTGGTTCAAATCCCCCAACTCACATATTGTCAAAAAACACATACACACAAAGCTAAAAGTTTAGAGACCAAACTTGTGAAAATACACCACGCTATAAATTTAGAAAGATATATGCTAACATTAAAAAAAAATGACCATCAATTTTAATTAAATTACAAGTTTAGATTGTGTTTAATAAGCTTTCGAATTTTAGATGTGTTAGAAGTTCTTTTTTTTTTTAAACAAAATGTGTTGGAAGTTGTTTTTAATATATATTAGTTAAATTTTTGGAAATAAGAAATTTTAGGGCCCTCCTACTTTTATTTGTACTCAATCGTACCGACAATAAGAAATTGCCATTAAATTATTATGGCAGAGTTTTAAATTTTATATATGTAACAATTAAAATTACAAAATTTTAGGTGCATGTATTTTTTATCCATTGGTGAAAAAAAAAACACCTAAATCTTATCTCTTAACTAGAAATAAATATTGAGCTGTAATTGCTGCATTTGAAAATGAAACCCATGTGGTGTGACATGGGAATTGGGATTCAATAAGTCACACATTTTCATTAAAAAAAAAAAAAAAAAGTCACACATTTTCATTTTCATTGTTCGTATTTCTTTTATAATTTCAATTCTATTTTTGACTAATTTTCGTCAACAAATGAAAATCACAAACACATCCATAAATATAATTTTTAAAAATTCCATAAATAAATTATTTGTTATATATTTTTTAAAATTCCACTTCTAGTGGACAAATTATTTTATCTTTTCTTAAAGGTTTTTTTTTTTTAAAAAAAACCTTGATATTATTACCGACACTATGAAATTGCCATTATTCACAAATATCTAATAAATCTTCAAACTTTTAATATTATGTATGTCTAATAGTTCCTAATATATTTAAAAACTTTAATATTATAAAATTGAATTTTTGAATCTTAAAGTGATCTTACCTACAATTGATCGTTTAAAATTTTGTAAAATTTGGAGAAATTTTTAGGTGCTTCCAAGCAAGACTATTAGTTTACATTTTGGATGTAAGAGAAAGAAATGAAGTTTCTAGATTTTGTATCTAGAGTTATTAGGGATAAATAAATTTGAAAATTTACATCTGACCATTTGAAAAGGAGAGTCTATGTACTGGCCTAATAATTTTCGACAATTTGACACTAAATTTCATGTTCCTCTGTTACGTGGATGTAAAATTTGCAGCGGCGGTGGGAGAAAGATTCTTAGCGAGGGACATCACGTTCCAGAACACCGCCGGTCCATCGAAGCACCAAGCGGTGGCGCTCCGCGTCGGCTCCGATTTCTCCGCCTTTTACCGCTGCGACATGCTCGCCTATCAAGACACTCTCTACGTCCACTCCAACCGCCAATTCTACGTCAGCTGCATCATCATCGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGCCGTCATCCAAGACTGCGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAGAAACATGGTCACTGCACAGGGAAGAACCGACCCAAATCAGAACACCGGAATCGTGATCCAGAAGTGCCGAATCGGAACCACCTCCGATCTGCAGCCGGTGATCAGAAATTTCCCGACGTTTCTCGGCCGGCCGTGGCAGAGATATTCGAGGACGGTGGTTATGCAGACGAGTATTAGCAACGTGATTGATCCGGCGGGTTGGCATGTTTGGGATGGGAATTTTGCGCTCGATACTCTGTTTTATGCCGAGTATCAGAACAGTGGGCCTGGCGCCGATACGTCGAGGAGAGTGACGTGGAAGGGGTACAGGGTCCTTACCAGTGCGGCGGAAGCGCAGGAATTCACGGCCGGAAATTTCATCGGCGGCGGCAGCTGGCTGAGCTCCACCGGATTCCCGTTTTCACTTGGGCTGTAA

mRNA sequence

ATGGCACAGCCCGACGATTCCGCCGCCGCCGGAGTTCCCTTCTCCGGCAGCCCCAAAAAGAAGAACCTCTTCATCCTAGCCCTCCTCCTCGTCATCGGCACTGTCGCCGGCATCTCCTCCTCGGAAGACGACCACGTGACCGTCGTGAGAAGCACGTGCGCCGAGACGCTCTACCCGGAGCTGTGCCAGTCGACGATATCCGCCGCCGTGCGAGGTGGTTCGAAGAAGGTGTCGAGTTCGAAAGACGTAATCGAAGCGTCGGTGAACATCACGATCGGTGCAGTGAAGCGGAACTACAAAAGCATAAAGAAATTGGTTGCGACGAGGAAGAATCTGAGCAAGCGAGAGAAAATTGCTCTGGAGGATTGTCTGGAGAACGGCGAGGAGACGCTGAGAGAGCTGTATGAAGTGGTTGAAGATCTTAATGAGTATCCTAAGAAGAAGGCTCTGTCGCAGTACGCCGATGACCTCAAAACCTTGCTCAGTTCCGCCATTACTAATCAGGAGACATGTCTGGACGGATTCTCGCACGACAAAGCCGACAAGATCGTGCGAAAGTCATTAGAATCCGGCCAAATCCACGTCGAGAAACTCTGCAGCATCGCTTTAGCATTGATCAAGAATCTCACCGACACCGACATTGCCAATCGGAACAACAACCTCGTCAGAAAGCAGCTGACGGAGGACGACGGAATTCAGTGGCCGGATTGGCTGCCTGCCGGTGACCGGAGGCTTTTGCAGGCGTCGTCGGCGGCGACTCCGGACGCGGTGGTTGCCGCCGACGGGAGCGGAGATTTCACGACGGTGTCGGCGGCGGTAGCGGCGGCGCCGAATGGGAGTAGCCGGAGATATATAATAAGAATTAAGGCGGGGGTTTATAGAGAGAATGTGAATGTGGCGAGTGGCAAGAGGAATATAATGTTTTGGGGAGATGGAAGAACCACTACTATTATCACCGGAAATAGAAATGTCGTCGACGGCAGCACCACCTTCAACTCCGCCACCGTTGCGGCGGTGGGAGAAAGATTCTTAGCGAGGGACATCACGTTCCAGAACACCGCCGGTCCATCGAAGCACCAAGCGGTGGCGCTCCGCGTCGGCTCCGATTTCTCCGCCTTTTACCGCTGCGACATGCTCGCCTATCAAGACACTCTCTACGTCCACTCCAACCGCCAATTCTACGTCAGCTGCATCATCATCGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGCCGTCATCCAAGACTGCGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAGAAACATGGTCACTGCACAGGGAAGAACCGACCCAAATCAGAACACCGGAATCGTGATCCAGAAGTGCCGAATCGGAACCACCTCCGATCTGCAGCCGGTGATCAGAAATTTCCCGACGTTTCTCGGCCGGCCGTGGCAGAGATATTCGAGGACGGTGGTTATGCAGACGAGTATTAGCAACGTGATTGATCCGGCGGGTTGGCATGTTTGGGATGGGAATTTTGCGCTCGATACTCTGTTTTATGCCGAGTATCAGAACAGTGGGCCTGGCGCCGATACGTCGAGGAGAGTGACGTGGAAGGGGTACAGGGTCCTTACCAGTGCGGCGGAAGCGCAGGAATTCACGGCCGGAAATTTCATCGGCGGCGGCAGCTGGCTGAGCTCCACCGGATTCCCGTTTTCACTTGGGCTGTAA

Coding sequence (CDS)

ATGGCACAGCCCGACGATTCCGCCGCCGCCGGAGTTCCCTTCTCCGGCAGCCCCAAAAAGAAGAACCTCTTCATCCTAGCCCTCCTCCTCGTCATCGGCACTGTCGCCGGCATCTCCTCCTCGGAAGACGACCACGTGACCGTCGTGAGAAGCACGTGCGCCGAGACGCTCTACCCGGAGCTGTGCCAGTCGACGATATCCGCCGCCGTGCGAGGTGGTTCGAAGAAGGTGTCGAGTTCGAAAGACGTAATCGAAGCGTCGGTGAACATCACGATCGGTGCAGTGAAGCGGAACTACAAAAGCATAAAGAAATTGGTTGCGACGAGGAAGAATCTGAGCAAGCGAGAGAAAATTGCTCTGGAGGATTGTCTGGAGAACGGCGAGGAGACGCTGAGAGAGCTGTATGAAGTGGTTGAAGATCTTAATGAGTATCCTAAGAAGAAGGCTCTGTCGCAGTACGCCGATGACCTCAAAACCTTGCTCAGTTCCGCCATTACTAATCAGGAGACATGTCTGGACGGATTCTCGCACGACAAAGCCGACAAGATCGTGCGAAAGTCATTAGAATCCGGCCAAATCCACGTCGAGAAACTCTGCAGCATCGCTTTAGCATTGATCAAGAATCTCACCGACACCGACATTGCCAATCGGAACAACAACCTCGTCAGAAAGCAGCTGACGGAGGACGACGGAATTCAGTGGCCGGATTGGCTGCCTGCCGGTGACCGGAGGCTTTTGCAGGCGTCGTCGGCGGCGACTCCGGACGCGGTGGTTGCCGCCGACGGGAGCGGAGATTTCACGACGGTGTCGGCGGCGGTAGCGGCGGCGCCGAATGGGAGTAGCCGGAGATATATAATAAGAATTAAGGCGGGGGTTTATAGAGAGAATGTGAATGTGGCGAGTGGCAAGAGGAATATAATGTTTTGGGGAGATGGAAGAACCACTACTATTATCACCGGAAATAGAAATGTCGTCGACGGCAGCACCACCTTCAACTCCGCCACCGTTGCGGCGGTGGGAGAAAGATTCTTAGCGAGGGACATCACGTTCCAGAACACCGCCGGTCCATCGAAGCACCAAGCGGTGGCGCTCCGCGTCGGCTCCGATTTCTCCGCCTTTTACCGCTGCGACATGCTCGCCTATCAAGACACTCTCTACGTCCACTCCAACCGCCAATTCTACGTCAGCTGCATCATCATCGGCACCGTCGACTTCATCTTCGGCAACGCCGCCGCCGTCATCCAAGACTGCGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAGAAACATGGTCACTGCACAGGGAAGAACCGACCCAAATCAGAACACCGGAATCGTGATCCAGAAGTGCCGAATCGGAACCACCTCCGATCTGCAGCCGGTGATCAGAAATTTCCCGACGTTTCTCGGCCGGCCGTGGCAGAGATATTCGAGGACGGTGGTTATGCAGACGAGTATTAGCAACGTGATTGATCCGGCGGGTTGGCATGTTTGGGATGGGAATTTTGCGCTCGATACTCTGTTTTATGCCGAGTATCAGAACAGTGGGCCTGGCGCCGATACGTCGAGGAGAGTGACGTGGAAGGGGTACAGGGTCCTTACCAGTGCGGCGGAAGCGCAGGAATTCACGGCCGGAAATTTCATCGGCGGCGGCAGCTGGCTGAGCTCCACCGGATTCCCGTTTTCACTTGGGCTGTAA

Protein sequence

MAQPDDSAAAGVPFSGSPKKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAETLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL
Homology
BLAST of Lag0016880 vs. NCBI nr
Match: XP_023526589.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 931.4 bits (2406), Expect = 3.7e-267
Identity = 481/571 (84.24%), Postives = 510/571 (89.32%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSP-----KKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAE 60
           MAQ DDS  AG P +GSP     KK NLFILAL   +  +AG+SS  DDHVT VRSTCA 
Sbjct: 1   MAQTDDSTVAGAPSTGSPTSISNKKHNLFILALSAAV-LLAGVSS--DDHVTAVRSTCAV 60

Query: 61  TLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKR 120
           TLYPELCQSTIS AV   SKKVSSSKD+IEASVNITI AVK+NYKSIKKL+ T K+L+KR
Sbjct: 61  TLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKR 120

Query: 121 EKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGF 180
           EKIAL DCLE GEETLRELYEVVEDLNEYPKKK+L QYADDLKTLLSSAITNQETC+DGF
Sbjct: 121 EKIALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGF 180

Query: 181 SHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQWP 240
           SHD+ADK +R+SL +GQIHVEKLCS+ALALIKNLTDTDIAN  N  +R +        WP
Sbjct: 181 SHDRADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYN--IRGRSMAMSEFNWP 240

Query: 241 DWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRE 300
           DW+  GDRRLLQASS ATPD VVAADGSGDF TVSAAVAAAP+ SSRRYIIRIKAGVYRE
Sbjct: 241 DWMSPGDRRLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300

Query: 301 NVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAG 360
           NVNV S K NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARDITFQNTAG
Sbjct: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 360

Query: 361 PSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQ 420
           PSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYVSCII+GTVDFIFGNAAAVIQ
Sbjct: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420

Query: 421 DCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480
           + DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ
Sbjct: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480

Query: 481 RYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLT 540
           RYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSG GADTSRRVTWKGYRV++
Sbjct: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVIS 540

Query: 541 SAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           SAA+A+ FTAG FI GG+WLSSTGFPFSLGL
Sbjct: 541 SAADAESFTAGKFISGGTWLSSTGFPFSLGL 566

BLAST of Lag0016880 vs. NCBI nr
Match: XP_022980581.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 927.9 bits (2397), Expect = 4.1e-266
Identity = 480/575 (83.48%), Postives = 510/575 (88.70%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSP-----KKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAE 60
           MAQ DDS  AG P + SP     KK NLFILAL   +  + G+SS  DDHV  VRSTCA 
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAAL-LLDGVSS--DDHVAAVRSTCAV 60

Query: 61  TLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKR 120
           TLYPELCQSTIS AV   SKKVSSSKD+IEASVNITI AVK+NYKSIKKLV T KNL+KR
Sbjct: 61  TLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKR 120

Query: 121 EKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGF 180
           EKIAL DCLE GEETLRELYEVVEDLNEYPKKK+L QYADDLKTLLSSAITNQETC+DGF
Sbjct: 121 EKIALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGF 180

Query: 181 SHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANR----NNNLVRKQLTEDDG 240
           SHD+ADK +R+SL +GQIHVEKLCS+ALALIKNLTDTDIAN      NN+  + +   + 
Sbjct: 181 SHDRADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE- 240

Query: 241 IQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAG 300
             WPDW+  GDR+LLQASS ATPD VVAADGSGDF TVSAAVAAAP+ SSRRYIIRIKAG
Sbjct: 241 FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ 360
           VYRENVNV S K NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ
Sbjct: 301 VYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ 360

Query: 361 NTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAA 420
           NTAGPSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYVSCII+GTVDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480
           AVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG
Sbjct: 421 AVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480

Query: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGY 540
           RPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAE+QNSG GADTSRRVTWKGY
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKGY 540

Query: 541 RVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           RV++SAAEA+ FTAG FI GG+WLSSTGFPFSLGL
Sbjct: 541 RVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

BLAST of Lag0016880 vs. NCBI nr
Match: KAG6581794.1 (Pectinesterase/pectinesterase inhibitor U1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 926.4 bits (2393), Expect = 1.2e-265
Identity = 478/571 (83.71%), Postives = 508/571 (88.97%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSP-----KKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAE 60
           MAQ DDS  AG P +G P     KK NLFI+ L   +  +AG+SS  +DHVT VRSTCA 
Sbjct: 1   MAQTDDSPVAGAPITGCPTSISNKKHNLFIIVLSAAL-LLAGVSS--EDHVTAVRSTCAV 60

Query: 61  TLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKR 120
           TLYPELCQSTIS AV   SKKVSSSKD+IEASVNITI AVK+NYKSIKKL+ T K+L+KR
Sbjct: 61  TLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKR 120

Query: 121 EKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGF 180
           EKIAL DCLE GEETLRELYEVVEDLNEYPKKK+L QYADDLKTLLSSAITNQETC+DGF
Sbjct: 121 EKIALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGF 180

Query: 181 SHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQWP 240
           SHD+ADK VR+SL +GQIHVEKLCS+ALALIKNLTDTDIAN  N  +R +        WP
Sbjct: 181 SHDRADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYN--IRGRSMAMSEFNWP 240

Query: 241 DWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRE 300
           DW+  GDR+LLQASS ATPD VVAADGSGDF TVSAAVAAAP+ SSRRYIIRIKAGVYRE
Sbjct: 241 DWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300

Query: 301 NVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAG 360
           NVNV S K NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARDITFQNTAG
Sbjct: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 360

Query: 361 PSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQ 420
           PSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYVSCII+GTVDFIFGNAAAVIQ
Sbjct: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420

Query: 421 DCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480
           + DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ
Sbjct: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480

Query: 481 RYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLT 540
           RYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSG GADTSRRVTWKGYRV++
Sbjct: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVIS 540

Query: 541 SAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           SAAEA+ FTAG FI GG+WLSSTGFPFSLGL
Sbjct: 541 SAAEAESFTAGKFISGGTWLSSTGFPFSLGL 566

BLAST of Lag0016880 vs. NCBI nr
Match: XP_022955430.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 924.9 bits (2389), Expect = 3.4e-265
Identity = 476/571 (83.36%), Postives = 508/571 (88.97%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSP-----KKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAE 60
           MAQ DDS  AG P +G P     KK NLFI+ L   +  +AG+SS  +DHVT VRSTCA 
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAAL-LLAGVSS--EDHVTAVRSTCAV 60

Query: 61  TLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKR 120
           TLYPELCQSTIS AV   SKKVSSSKD+IEASVNITI AVK+NYKSIKKL+ T K+L+KR
Sbjct: 61  TLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKR 120

Query: 121 EKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGF 180
           EKIAL DCLE GEETLRELYEVVEDLNEYPKKK+L QYADDLKTLLSSAITNQETC+DGF
Sbjct: 121 EKIALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGF 180

Query: 181 SHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQWP 240
           SHD+ADK VR+SL +GQ HVEKLCS+ALALIKNLTDTDIAN  N  +R +        WP
Sbjct: 181 SHDRADKKVRESLLAGQFHVEKLCSVALALIKNLTDTDIANHYN--IRGRSMAMSEFNWP 240

Query: 241 DWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRE 300
           DW+  GDR+LLQASS ATPDAVVAADGSGDF TVSAAVAAAP+ SSRRYIIRIKAGVYRE
Sbjct: 241 DWMSPGDRKLLQASSTATPDAVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300

Query: 301 NVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAG 360
           NVNV S K NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARDITFQNTAG
Sbjct: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 360

Query: 361 PSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQ 420
           PSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYVSCII+GTVDFIFGNAAAVIQ
Sbjct: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420

Query: 421 DCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480
           + DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ
Sbjct: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480

Query: 481 RYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLT 540
           RYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQN+G GADTSRRVTWKGYR+++
Sbjct: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNNGAGADTSRRVTWKGYRIIS 540

Query: 541 SAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           SAAEA+ FTAG FI GG+WLSSTGFPFSLGL
Sbjct: 541 SAAEAESFTAGKFISGGTWLSSTGFPFSLGL 566

BLAST of Lag0016880 vs. NCBI nr
Match: KAG7018245.1 (Pectinesterase/pectinesterase inhibitor U1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 924.5 bits (2388), Expect = 4.5e-265
Identity = 477/571 (83.54%), Postives = 508/571 (88.97%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSP-----KKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAE 60
           MAQ DDS  AG P +G P     KK NLFI+ L   +  +AGISS  +DHVT VRSTCA 
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAAL-LLAGISS--EDHVTAVRSTCAV 60

Query: 61  TLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKR 120
           TLYPELCQSTIS AV   SKKVSSSKD+IEASVNITI AVK+NYKSI+KL+ T K+L+KR
Sbjct: 61  TLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIEKLLETTKHLTKR 120

Query: 121 EKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGF 180
           EKIA+ DCLE GEETLRELYEVVEDLNEYPKKK+L QYADDLKTLLSSAITNQETC+DGF
Sbjct: 121 EKIAVHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGF 180

Query: 181 SHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQWP 240
           SHD+ADK VR+SL +GQIHVEKLCS+ALALIKNLTDTDIAN  N  +R +        WP
Sbjct: 181 SHDRADKKVRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYN--IRGRSMAMSEFNWP 240

Query: 241 DWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRE 300
           DW+  GDR+LLQASS ATPD VVAADGSGDF TVSAAVAAAP+ SSRRYIIRIKAGVYRE
Sbjct: 241 DWMSTGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300

Query: 301 NVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAG 360
           NVNV S K NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARDITFQNTAG
Sbjct: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 360

Query: 361 PSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQ 420
           PSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYVSCII+GTVDFIFGNAAAVIQ
Sbjct: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420

Query: 421 DCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480
           + DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ
Sbjct: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480

Query: 481 RYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLT 540
           RYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSG GADTSRRVTWKGYRV++
Sbjct: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKGYRVIS 540

Query: 541 SAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           SAAEA+ FTAG FI GG+WLSSTGFPFSLGL
Sbjct: 541 SAAEAESFTAGKFISGGTWLSSTGFPFSLGL 566

BLAST of Lag0016880 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 740.3 bits (1910), Expect = 1.6e-212
Identity = 389/569 (68.37%), Postives = 454/569 (79.79%), Query Frame = 0

Query: 15  SGSPKKKNLFILALL-------LVIGTVAGISS-------SEDDHVTVVRSTCAETLYPE 74
           S  PKKK    LAL         VIG VAG++S         + H  +++S+C+ T YP+
Sbjct: 21  SNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPD 80

Query: 75  LCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIAL 134
           LC S I AAV   SKKV+S KDVIE S+NIT  AV+ NY  I+KL+  R NL+KREK+AL
Sbjct: 81  LCFSAI-AAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLL-KRTNLTKREKVAL 140

Query: 135 EDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKA 194
            DCLE  +ETL EL++ VEDL EYP KK+LSQ+ADDLKTL+S+A+TNQ TCLDGFSHD A
Sbjct: 141 HDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDA 200

Query: 195 DKIVRKSLESGQIHVEKLCSIALALIKNLTDTD---IANRNNNLVRKQLTEDDGIQWPDW 254
           +K VR +L  GQ+HVEK+CS ALA+IKN+TDTD   +   NN  + ++ +  DG  WP W
Sbjct: 201 NKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLTEETSTVDG--WPAW 260

Query: 255 LPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRENV 314
           L  GDRRLLQ SS+ TP+AVVAADGSG+F TV+AAVAAAP G ++RYIIRIKAGVYRENV
Sbjct: 261 LSPGDRRLLQ-SSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAGVYRENV 320

Query: 315 NVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAGPS 374
            V    +NIMF GDGRT TIITG+RNVVDGSTTF SAT A VGE FLARDITFQNTAGPS
Sbjct: 321 EVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITFQNTAGPS 380

Query: 375 KHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQDC 434
           KHQAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF+V+C+I GTVDFIFGNAAAV+Q+C
Sbjct: 381 KHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNC 440

Query: 435 DIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRY 494
           DIHAR+PN GQ+NMVTAQGRTDPNQNTGIVIQK RIG TSDL+PV  +FPT+LGRPW+ Y
Sbjct: 441 DIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEY 500

Query: 495 SRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLTSA 554
           SRTV+MQ+SI+++I PAGWH WDGNFAL+TLFY E+QNSG GA TS RV WKG+RV+TSA
Sbjct: 501 SRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKGFRVITSA 560

Query: 555 AEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
            EAQ FT G+FI G SWL STGFPFSLGL
Sbjct: 561 TEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lag0016880 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 1.0e-211
Identity = 386/567 (68.08%), Postives = 451/567 (79.54%), Query Frame = 0

Query: 15  SGSPKKKNLFILALL-------LVIGTVAGISS-------SEDDHVTVVRSTCAETLYPE 74
           S  PKKK    LAL         VIG VAG++S         + H  +++S+C+ T YP+
Sbjct: 21  SNIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPD 80

Query: 75  LCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIAL 134
           LC S I AAV   SKKV+S KDVIE S+NIT  AV+ NY  I+KL+  R NL+KREK+AL
Sbjct: 81  LCFSAI-AAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLL-KRTNLTKREKVAL 140

Query: 135 EDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKA 194
            DCLE  +ETL EL++ VEDL EYP KK+LSQ+ADDLKTL+S+A+TNQ TCLDGFSHD A
Sbjct: 141 HDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDA 200

Query: 195 DKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQ-WPDWLP 254
           +K VR +L  GQ+HVEK+CS ALA+IKN+TDTD+     +  RK + E   +  WP WL 
Sbjct: 201 NKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETSTVDGWPAWLS 260

Query: 255 AGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRENVNV 314
            GDRRLLQ SS+ TP+ VVAADGSG+F TV+A+VAAAP G ++RYIIRIKAGVYRENV V
Sbjct: 261 TGDRRLLQ-SSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIKAGVYRENVEV 320

Query: 315 ASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAGPSKH 374
               +NIMF GDGRT TIITG+RNVVDGSTTF SATVA VGE FLARDITFQNTAGPSKH
Sbjct: 321 TKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDITFQNTAGPSKH 380

Query: 375 QAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQDCDI 434
           QAVALRVG+D SAFY CDMLAYQDTLYVHSNRQF+V+C+I GTVDFIFGNAAAV+Q+CDI
Sbjct: 381 QAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNAAAVLQNCDI 440

Query: 435 HARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSR 494
           HAR+PN GQ+NMVTAQGR DPNQNTGIVIQK RIG TSDL+PV  +FPT+LGRPW+ YSR
Sbjct: 441 HARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPTYLGRPWKEYSR 500

Query: 495 TVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLTSAAE 554
           TV+MQ+SI++VI PAGWH WDGNFAL+TLFY E+QN+G GA TS RV WKG+RV+TSA E
Sbjct: 501 TVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKWKGFRVITSATE 560

Query: 555 AQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           AQ FT G+FI G SWL STGFPFSLGL
Sbjct: 561 AQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Lag0016880 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 732.6 bits (1890), Expect = 3.3e-210
Identity = 386/586 (65.87%), Postives = 454/586 (77.47%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSPKKKNLFILALLL----VIGTVAGI---SSSEDDHV------- 60
           M + +D  +  + F    KK  L I+A +L    VIG VAG+   S + DDH        
Sbjct: 1   MTRVEDFFSKQIDFCKRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISS 60

Query: 61  ---TVVRSTCAETLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIK 120
               +V+S C+ TL+PELC S I   V   SKKV+S KDVIE S+NIT+ AV+RNY ++K
Sbjct: 61  SAHAIVKSACSNTLHPELCYSAI-VNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVK 120

Query: 121 KLVATRKNLSKREKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSS 180
           +L+ TRK L+ REK+AL DCLE  +ETL EL+  VEDL  YP KK+L ++ +DLKTL+SS
Sbjct: 121 ELIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISS 180

Query: 181 AITNQETCLDGFSHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANR---NNN 240
           AITNQETCLDGFSHD+ADK VRK L  GQ HVEK+CS ALA+I N+TDTDIAN    +  
Sbjct: 181 AITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIANEMKLSAP 240

Query: 241 LVRKQLTEDDGIQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGS 300
              ++L ED+G +WP+WL AGDRRLLQ SS  TPD VVAADGSGD+ TVS AV  AP  S
Sbjct: 241 ANNRKLVEDNG-EWPEWLSAGDRRLLQ-SSTVTPDVVVAADGSGDYKTVSEAVRKAPEKS 300

Query: 301 SRRYIIRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG 360
           S+RY+IRIKAGVYRENV+V   K NIMF GDG++ TIIT +RNV DGSTTF+SATV  V 
Sbjct: 301 SKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVA 360

Query: 361 ERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIII 420
            + LARDITFQNTAG SKHQAVAL VGSD SAFYRCDMLAYQDTLYVHSNRQF+V C++ 
Sbjct: 361 GKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVA 420

Query: 421 GTVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQ 480
           GTVDFIFGN AAV QDCDIHARRP  GQ+NMVTAQGRTDPNQNTGIVIQKCRIG TSDL+
Sbjct: 421 GTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLR 480

Query: 481 PVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGA 540
           PV ++FPT+LGRPW+ YSRTV+MQ+SI++VI PAGWH W+GNFALDTLFY EY N+G GA
Sbjct: 481 PVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGA 540

Query: 541 DTSRRVTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
            TS RV WKG++V+TS+ EAQ +T G FI GGSWLSSTGFPFSLGL
Sbjct: 541 PTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Lag0016880 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 730.7 bits (1885), Expect = 1.2e-209
Identity = 389/582 (66.84%), Postives = 448/582 (76.98%), Query Frame = 0

Query: 19  KKKNLFILA---LLLVIGTVAGISS-----------SEDDHVTVVRSTCAETLYPELCQS 78
           K K L +L+    LL +  VAGIS+           S   H  V+RS+C+ T YPELC  
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSH-AVLRSSCSSTRYPELC-- 75

Query: 79  TISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIALEDCL 138
            ISA V  G  +++S KDVIEASVN+TI AV+ NY ++KKL+  RK L+ REK AL DCL
Sbjct: 76  -ISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCL 135

Query: 139 ENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKADKIV 198
           E  +ETL EL+E VEDL+ YP KK L ++A DLKTL+SSAITNQETCLDGFSHD ADK V
Sbjct: 136 ETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQV 195

Query: 199 RKSLESGQIHVEKLCSIALALIKNLTDTDIAN--------RNNNLVRKQLTED------- 258
           RK+L  GQIHVE +CS ALA+IKN+TDTDIAN         NN  ++++  E        
Sbjct: 196 RKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIA 255

Query: 259 -----DGIQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRY 318
                D   WP WL AGDRRLLQ S     DA VAADGSG F TV+AAVAAAP  S++RY
Sbjct: 256 GAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTFKTVAAAVAAAPENSNKRY 315

Query: 319 IIRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFL 378
           +I IKAGVYRENV VA  K+NIMF GDGRT TIITG+RNVVDGSTTF+SATVAAVGERFL
Sbjct: 316 VIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFL 375

Query: 379 ARDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVD 438
           ARDITFQNTAGPSKHQAVALRVGSDFSAFY CDMLAYQDTLYVHSNRQF+V C+I GTVD
Sbjct: 376 ARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVD 435

Query: 439 FIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIR 498
           FIFGNAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGIVIQKCRIG TSDLQ V  
Sbjct: 436 FIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKG 495

Query: 499 NFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSR 558
           +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW  W G FAL+TL Y EY N+G GA T+ 
Sbjct: 496 SFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTAN 555

Query: 559 RVTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           RV W+G++V+T+AAEAQ++TAG FIGGG WLSSTGFPFSLGL
Sbjct: 556 RVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Lag0016880 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 709.5 bits (1830), Expect = 3.0e-203
Identity = 375/581 (64.54%), Postives = 446/581 (76.76%), Query Frame = 0

Query: 14  FSGSPKKKNLFILA---LLLVIGTVAGISS-----SEDDHVT--------VVRSTCAETL 73
           FS     K L + +    LL++ ++ GI++     +++  +T        +++S C+ TL
Sbjct: 11  FSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTL 70

Query: 74  YPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREK 133
           YPELC S ++A    G K+++S K+VIEAS+N+T  AVK NY ++KKL+A RK L+ RE 
Sbjct: 71  YPELCFSAVAAT---GGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREV 130

Query: 134 IALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSH 193
            AL DCLE  +ETL EL+  VEDL++YPK+K+L ++ADDLKTL+SSAITNQ TCLDGFS+
Sbjct: 131 TALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSY 190

Query: 194 DKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIAN-----------RNNNLVRKQL 253
           D AD+ VRK+L  GQ+HVE +CS ALA+IKN+T+TDIAN            NNN   K++
Sbjct: 191 DDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEV 250

Query: 254 TED-DGIQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYI 313
           T D D   WP WL  GDRRLLQ S+    DA VA DGSGDFTTV+AAVAAAP  S++R++
Sbjct: 251 TGDLDSDGWPKWLSVGDRRLLQGSTIKA-DATVADDGSGDFTTVAAAVAAAPEKSNKRFV 310

Query: 314 IRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLA 373
           I IKAGVYRENV V   K NIMF GDGR  TIITG+RNVVDGSTTF+SATVAAVGERFLA
Sbjct: 311 IHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLA 370

Query: 374 RDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDF 433
           RDITFQNTAGPSKHQAVALRVGSDFSAFY+CDM AYQDTLYVHSNRQF+V C I GTVDF
Sbjct: 371 RDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDF 430

Query: 434 IFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRN 493
           IFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVIQ CRIG TSDL  V   
Sbjct: 431 IFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGT 490

Query: 494 FPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRR 553
           FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL Y EY N G GA T+ R
Sbjct: 491 FPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANR 550

Query: 554 VTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           V WKGY+V+TS  EAQ FTAG FIGGG WL+STGFPFSL L
Sbjct: 551 VKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Lag0016880 vs. ExPASy TrEMBL
Match: A0A6J1ITZ4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1)

HSP 1 Score: 927.9 bits (2397), Expect = 2.0e-266
Identity = 480/575 (83.48%), Postives = 510/575 (88.70%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSP-----KKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAE 60
           MAQ DDS  AG P + SP     KK NLFILAL   +  + G+SS  DDHV  VRSTCA 
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAAL-LLDGVSS--DDHVAAVRSTCAV 60

Query: 61  TLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKR 120
           TLYPELCQSTIS AV   SKKVSSSKD+IEASVNITI AVK+NYKSIKKLV T KNL+KR
Sbjct: 61  TLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKR 120

Query: 121 EKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGF 180
           EKIAL DCLE GEETLRELYEVVEDLNEYPKKK+L QYADDLKTLLSSAITNQETC+DGF
Sbjct: 121 EKIALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGF 180

Query: 181 SHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANR----NNNLVRKQLTEDDG 240
           SHD+ADK +R+SL +GQIHVEKLCS+ALALIKNLTDTDIAN      NN+  + +   + 
Sbjct: 181 SHDRADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE- 240

Query: 241 IQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAG 300
             WPDW+  GDR+LLQASS ATPD VVAADGSGDF TVSAAVAAAP+ SSRRYIIRIKAG
Sbjct: 241 FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ 360
           VYRENVNV S K NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ
Sbjct: 301 VYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ 360

Query: 361 NTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAA 420
           NTAGPSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYVSCII+GTVDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480
           AVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG
Sbjct: 421 AVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480

Query: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGY 540
           RPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAE+QNSG GADTSRRVTWKGY
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKGY 540

Query: 541 RVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           RV++SAAEA+ FTAG FI GG+WLSSTGFPFSLGL
Sbjct: 541 RVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

BLAST of Lag0016880 vs. ExPASy TrEMBL
Match: A0A6J1GV42 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1)

HSP 1 Score: 924.9 bits (2389), Expect = 1.7e-265
Identity = 476/571 (83.36%), Postives = 508/571 (88.97%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSP-----KKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAE 60
           MAQ DDS  AG P +G P     KK NLFI+ L   +  +AG+SS  +DHVT VRSTCA 
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAAL-LLAGVSS--EDHVTAVRSTCAV 60

Query: 61  TLYPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKR 120
           TLYPELCQSTIS AV   SKKVSSSKD+IEASVNITI AVK+NYKSIKKL+ T K+L+KR
Sbjct: 61  TLYPELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKR 120

Query: 121 EKIALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGF 180
           EKIAL DCLE GEETLRELYEVVEDLNEYPKKK+L QYADDLKTLLSSAITNQETC+DGF
Sbjct: 121 EKIALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGF 180

Query: 181 SHDKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQWP 240
           SHD+ADK VR+SL +GQ HVEKLCS+ALALIKNLTDTDIAN  N  +R +        WP
Sbjct: 181 SHDRADKKVRESLLAGQFHVEKLCSVALALIKNLTDTDIANHYN--IRGRSMAMSEFNWP 240

Query: 241 DWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRE 300
           DW+  GDR+LLQASS ATPDAVVAADGSGDF TVSAAVAAAP+ SSRRYIIRIKAGVYRE
Sbjct: 241 DWMSPGDRKLLQASSTATPDAVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKAGVYRE 300

Query: 301 NVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAG 360
           NVNV S K NIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVG RFLARDITFQNTAG
Sbjct: 301 NVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITFQNTAG 360

Query: 361 PSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQ 420
           PSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYVSCII+GTVDFIFGNAAAVIQ
Sbjct: 361 PSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNAAAVIQ 420

Query: 421 DCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480
           + DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ
Sbjct: 421 NSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQ 480

Query: 481 RYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLT 540
           RYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQN+G GADTSRRVTWKGYR+++
Sbjct: 481 RYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNNGAGADTSRRVTWKGYRIIS 540

Query: 541 SAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           SAAEA+ FTAG FI GG+WLSSTGFPFSLGL
Sbjct: 541 SAAEAESFTAGKFISGGTWLSSTGFPFSLGL 566

BLAST of Lag0016880 vs. ExPASy TrEMBL
Match: A0A5A7UEV0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001090 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 9.8e-258
Identity = 468/572 (81.82%), Postives = 502/572 (87.76%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSPKKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAETLYPE 60
           M++PD++ AAGVPFS +  K NL + AL   +  +A +SS  DDH   VRS CA TLYPE
Sbjct: 1   MSKPDETLAAGVPFSCN-SKTNLLVFALSTTL-LLAAVSS--DDH---VRSKCAMTLYPE 60

Query: 61  LCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIAL 120
           LC++TIS AV       SSSK+ IEASVNITIGAV  NY+ +KKL+ T  +L+KREKIAL
Sbjct: 61  LCETTISTAVG------SSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIAL 120

Query: 121 EDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKA 180
            DCLE GEETLRELYEVVED+NEYP KK+LS+YADDLKTLLSSAITNQETCLDGFSHDKA
Sbjct: 121 HDCLETGEETLRELYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKA 180

Query: 181 DKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNN---LVRKQLTE---DDGIQW 240
           DK VR+SL+ G IH+EKLCSIALALIKNLTDTDIAN NNN   L RKQL E   DD I W
Sbjct: 181 DKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNHLSRKQLKEKNTDDRINW 240

Query: 241 PDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYR 300
           PDW+   DRRLLQASS ATPD VVAADGSGDF T+S AVAAAP+ SS+RYIIRIKAGVYR
Sbjct: 241 PDWMSPNDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYR 300

Query: 301 ENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTA 360
           ENVNVAS KRNIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQNTA
Sbjct: 301 ENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTA 360

Query: 361 GPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVI 420
           GPSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYV CII+GT+DFIFGNAAAVI
Sbjct: 361 GPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVI 420

Query: 421 QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPW 480
           QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLGRPW
Sbjct: 421 QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPW 480

Query: 481 QRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVL 540
           QRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSG GADTSRRV WKG+RVL
Sbjct: 481 QRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVL 540

Query: 541 TSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           T AAEA+ FTAGNFIGGG+WLSSTGFPFSLGL
Sbjct: 541 TRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 559

BLAST of Lag0016880 vs. ExPASy TrEMBL
Match: A0A1S3CCA5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1)

HSP 1 Score: 899.0 bits (2322), Expect = 9.8e-258
Identity = 468/572 (81.82%), Postives = 502/572 (87.76%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSPKKKNLFILALLLVIGTVAGISSSEDDHVTVVRSTCAETLYPE 60
           M++PD++ AAGVPFS +  K NL + AL   +  +A +SS  DDH   VRS CA TLYPE
Sbjct: 1   MSKPDETLAAGVPFSCN-SKTNLLVFALSTTL-LLAAVSS--DDH---VRSKCAMTLYPE 60

Query: 61  LCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIAL 120
           LC++TIS AV       SSSK+ IEASVNITIGAV  NY+ +KKL+ T  +L+KREKIAL
Sbjct: 61  LCETTISTAVG------SSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIAL 120

Query: 121 EDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKA 180
            DCLE GEETLRELYEVVED+NEYP KK+LS+YADDLKTLLSSAITNQETCLDGFSHDKA
Sbjct: 121 HDCLETGEETLRELYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKA 180

Query: 181 DKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNN---LVRKQLTE---DDGIQW 240
           DK VR+SL+ G IH+EKLCSIALALIKNLTDTDIAN NNN   L RKQL E   DD I W
Sbjct: 181 DKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNHLSRKQLKEKNTDDRINW 240

Query: 241 PDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYR 300
           PDW+   DRRLLQASS ATPD VVAADGSGDF T+S AVAAAP+ SS+RYIIRIKAGVYR
Sbjct: 241 PDWMSPNDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYR 300

Query: 301 ENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTA 360
           ENVNVAS KRNIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQNTA
Sbjct: 301 ENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTA 360

Query: 361 GPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVI 420
           GPSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYV CII+GT+DFIFGNAAAVI
Sbjct: 361 GPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVI 420

Query: 421 QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPW 480
           QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLGRPW
Sbjct: 421 QDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPW 480

Query: 481 QRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVL 540
           QRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSG GADTSRRV WKG+RVL
Sbjct: 481 QRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVL 540

Query: 541 TSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           T AAEA+ FTAGNFIGGG+WLSSTGFPFSLGL
Sbjct: 541 TRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 559

BLAST of Lag0016880 vs. ExPASy TrEMBL
Match: A0A0A0LCR6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1)

HSP 1 Score: 898.7 bits (2321), Expect = 1.3e-257
Identity = 467/575 (81.22%), Postives = 500/575 (86.96%), Query Frame = 0

Query: 1   MAQPDDSAAAGVPFSGSPKKKNL-FILALLLVIGTVAGISSSEDDHVTVVRSTCAETLYP 60
           M++PDD+ AAGVPFSG  K   L F L+  L++  V     S DDH   V S CA TLYP
Sbjct: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVV-----SSDDH---VGSKCAMTLYP 60

Query: 61  ELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIA 120
           ELC++TIS AV       SSSK+ IEASVNITI AVK NYK ++KL+ T KNL+KR+KIA
Sbjct: 61  ELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIA 120

Query: 121 LEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDK 180
             DCLE GEETLRELYEVVED+NEYPKKK+LS+YADDLKTLLSS ITNQETC+DGFSHDK
Sbjct: 121 FHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDK 180

Query: 181 ADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIANRNNN-----LVRKQLTE---DDG 240
            DK VR+SL+ G IH+EKLCSIALALIKNLTDTDIAN NNN     L RKQL E   +DG
Sbjct: 181 GDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDG 240

Query: 241 IQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAG 300
           I+WPDW+   DRRLLQASS ATPD VVAADGSGDF T+S AVAAAP+ SSRRYIIRIKAG
Sbjct: 241 IKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQ 360
           VYRENVNVAS KRNIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TFQ
Sbjct: 301 VYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQ 360

Query: 361 NTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAA 420
           NTAGPSKHQAVALRVGSD SAFYRCDMLAYQDTLYVHSNRQFYV CII+GT+DFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLG 480
           AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFLG
Sbjct: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLG 480

Query: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGY 540
           RPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSG GADTSRRV WKG+
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGF 540

Query: 541 RVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           RVLT AAEA+ FTAGNFIGGG+WLSSTGFPFSLGL
Sbjct: 541 RVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 561

BLAST of Lag0016880 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 730.7 bits (1885), Expect = 8.9e-211
Identity = 389/582 (66.84%), Postives = 448/582 (76.98%), Query Frame = 0

Query: 19  KKKNLFILA---LLLVIGTVAGISS-----------SEDDHVTVVRSTCAETLYPELCQS 78
           K K L +L+    LL +  VAGIS+           S   H  V+RS+C+ T YPELC  
Sbjct: 16  KNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSH-AVLRSSCSSTRYPELC-- 75

Query: 79  TISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIALEDCL 138
            ISA V  G  +++S KDVIEASVN+TI AV+ NY ++KKL+  RK L+ REK AL DCL
Sbjct: 76  -ISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCL 135

Query: 139 ENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKADKIV 198
           E  +ETL EL+E VEDL+ YP KK L ++A DLKTL+SSAITNQETCLDGFSHD ADK V
Sbjct: 136 ETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQV 195

Query: 199 RKSLESGQIHVEKLCSIALALIKNLTDTDIAN--------RNNNLVRKQLTED------- 258
           RK+L  GQIHVE +CS ALA+IKN+TDTDIAN         NN  ++++  E        
Sbjct: 196 RKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRKLKEENQETTVAVDIA 255

Query: 259 -----DGIQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRY 318
                D   WP WL AGDRRLLQ S     DA VAADGSG F TV+AAVAAAP  S++RY
Sbjct: 256 GAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTFKTVAAAVAAAPENSNKRY 315

Query: 319 IIRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFL 378
           +I IKAGVYRENV VA  K+NIMF GDGRT TIITG+RNVVDGSTTF+SATVAAVGERFL
Sbjct: 316 VIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATVAAVGERFL 375

Query: 379 ARDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVD 438
           ARDITFQNTAGPSKHQAVALRVGSDFSAFY CDMLAYQDTLYVHSNRQF+V C+I GTVD
Sbjct: 376 ARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVKCLIAGTVD 435

Query: 439 FIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIR 498
           FIFGNAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGIVIQKCRIG TSDLQ V  
Sbjct: 436 FIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLQSVKG 495

Query: 499 NFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSR 558
           +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW  W G FAL+TL Y EY N+G GA T+ 
Sbjct: 496 SFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNTGAGAGTAN 555

Query: 559 RVTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           RV W+G++V+T+AAEAQ++TAG FIGGG WLSSTGFPFSLGL
Sbjct: 556 RVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Lag0016880 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 709.5 bits (1830), Expect = 2.1e-204
Identity = 375/581 (64.54%), Postives = 446/581 (76.76%), Query Frame = 0

Query: 14  FSGSPKKKNLFILA---LLLVIGTVAGISS-----SEDDHVT--------VVRSTCAETL 73
           FS     K L + +    LL++ ++ GI++     +++  +T        +++S C+ TL
Sbjct: 11  FSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTL 70

Query: 74  YPELCQSTISAAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREK 133
           YPELC S ++A    G K+++S K+VIEAS+N+T  AVK NY ++KKL+A RK L+ RE 
Sbjct: 71  YPELCFSAVAAT---GGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREV 130

Query: 134 IALEDCLENGEETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSH 193
            AL DCLE  +ETL EL+  VEDL++YPK+K+L ++ADDLKTL+SSAITNQ TCLDGFS+
Sbjct: 131 TALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSY 190

Query: 194 DKADKIVRKSLESGQIHVEKLCSIALALIKNLTDTDIAN-----------RNNNLVRKQL 253
           D AD+ VRK+L  GQ+HVE +CS ALA+IKN+T+TDIAN            NNN   K++
Sbjct: 191 DDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELRDKSSTFTNNNNRKLKEV 250

Query: 254 TED-DGIQWPDWLPAGDRRLLQASSAATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYI 313
           T D D   WP WL  GDRRLLQ S+    DA VA DGSGDFTTV+AAVAAAP  S++R++
Sbjct: 251 TGDLDSDGWPKWLSVGDRRLLQGSTIKA-DATVADDGSGDFTTVAAAVAAAPEKSNKRFV 310

Query: 314 IRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLA 373
           I IKAGVYRENV V   K NIMF GDGR  TIITG+RNVVDGSTTF+SATVAAVGERFLA
Sbjct: 311 IHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLA 370

Query: 374 RDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDF 433
           RDITFQNTAGPSKHQAVALRVGSDFSAFY+CDM AYQDTLYVHSNRQF+V C I GTVDF
Sbjct: 371 RDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDF 430

Query: 434 IFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRN 493
           IFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVIQ CRIG TSDL  V   
Sbjct: 431 IFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGT 490

Query: 494 FPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRR 553
           FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL Y EY N G GA T+ R
Sbjct: 491 FPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANR 550

Query: 554 VTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLGL 567
           V WKGY+V+TS  EAQ FTAG FIGGG WL+STGFPFSL L
Sbjct: 551 VKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Lag0016880 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 421.4 bits (1082), Expect = 1.2e-117
Identity = 250/544 (45.96%), Postives = 340/544 (62.50%), Query Frame = 0

Query: 24  FILALLLVIGTVAGISSSEDDHVTVVRSTCAETLYPELCQSTISAAVRGGSKKVSSSKDV 83
           F++  +LV  TV+G +  +      V++ C++T  P+ C+  ++      ++ + S  + 
Sbjct: 11  FVILCILVASTVSGYNQKD------VKAWCSQTPNPKPCEYFLTH--NSNNEPIKSESEF 70

Query: 84  IEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIALEDCLENGEETLRELYEVVEDLNE 143
           ++ S+ + +   +             K    REK A EDC++  + T+ ++ E ++    
Sbjct: 71  LKISMKLVLD--RAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD---- 130

Query: 144 YPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKADKIVRKSLESGQIHVEKLCSIAL 203
            P  K       D +T LS+A+TN +TC  GF            LE G      +  I L
Sbjct: 131 -PNVKCSKL---DAQTWLSTALTNLDTCRAGF------------LELG------VTDIVL 190

Query: 204 ALIKNLTDTDIANR-NNNLVRKQLTEDDGIQWPDWLPAGDRRLLQASSAATPDAVVAADG 263
            L+ N     + N    N V    T  +   +P W+  GDR+LLQ SS    +AVVA DG
Sbjct: 191 PLMSNNVSNLLCNTLAINKVPFNYTPPEKDGFPSWVKPGDRKLLQ-SSTPKDNAVVAKDG 250

Query: 264 SGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNR 323
           SG+F T+  A+ AA    S R++I +K GVY EN+ +   K+N+M  GDG   TIITG++
Sbjct: 251 SGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEIR--KKNVMLRGDGIGKTIITGSK 310

Query: 324 NVVDGSTTFNSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQ 383
           +V  G+TTFNSATVAAVG+ F+AR ITF+NTAG S  QAVALR GSD S FY+C   AYQ
Sbjct: 311 SVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQ 370

Query: 384 DTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQ 443
           DTLYVHSNRQFY  C + GTVDFIFGNAAAV+Q+C+I ARRP   + N +TAQGR+DPNQ
Sbjct: 371 DTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPR-SKTNTITAQGRSDPNQ 430

Query: 444 NTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGN 503
           NTGI+I   R+   SDL+PV+ +  T+LGRPW++YSRTV M+TS+ ++IDP GW  WDGN
Sbjct: 431 NTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGN 490

Query: 504 FALDTLFYAEYQNSGPGADTSRRVTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPF 563
           FAL TLFYAE+QN+GPGA TS RVTW G+RVL SA+EA +FT G F+ GGSW+ S+  PF
Sbjct: 491 FALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS-VPF 511

Query: 564 SLGL 567
           + GL
Sbjct: 551 TSGL 511

BLAST of Lag0016880 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 412.5 bits (1059), Expect = 5.4e-115
Identity = 244/560 (43.57%), Postives = 336/560 (60.00%), Query Frame = 0

Query: 17  SPKKKNL-FILALLLVIGTVAGI--------SSSEDDHVTVVRSTCAETLYPELCQSTIS 76
           S K KNL  +L+ + ++G+VA          +++ DD +      C      + CQ+ +S
Sbjct: 14  SLKHKNLCLVLSFVAILGSVAFFTAQLISVNTNNNDDSLLTTSQICHGAHDQDSCQALLS 73

Query: 77  AAVRGGSKKVSSSKDVIEASVNITIGAVKRNYKSIKKLVATRKNLSKREKIALEDCLENG 136
                   K+ +  D++   +  ++  ++     + +  A  ++   R+K    DC E  
Sbjct: 74  EFTTLSLSKL-NRLDLLHVFLKNSVWRLESTMTMVSE--ARIRSNGVRDKAGFADCEEMM 133

Query: 137 EETLRELYEVVEDLNEYPKKKALSQYADDLKTLLSSAITNQETCLDGFSHDKAD--KIVR 196
           + +   +   +E+L        L  Y+ ++ T LSS +TN  TCL+  S    +  +IV+
Sbjct: 134 DVSKDRMMSSMEELR--GGNYNLESYS-NVHTWLSSVLTNYMTCLESISDVSVNSKQIVK 193

Query: 197 KSLESGQIHVEKLCSIALALIKNLTDTDIANRNNNLVRKQLTEDDGIQWPDWLPAGDRRL 256
             LE     +     +ALA+  ++             R  L      ++P WL A DR+L
Sbjct: 194 PQLED----LVSRARVALAIFVSVLP----------ARDDLKMIISNRFPSWLTALDRKL 253

Query: 257 LQASS---AATPDAVVAADGSGDFTTVSAAVAAAPNGSSRRYIIRIKAGVYRENVNVASG 316
           L++S      T + VVA DG+G F TV+ AVAAAP  S+ RY+I +K GVY+E +++   
Sbjct: 254 LESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKK 313

Query: 317 KRNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITFQNTAGPSKHQAV 376
           K+N+M  GDG+  TIITG+ NV+DGSTTF SATVAA G+ F+A+DI FQNTAGP+KHQAV
Sbjct: 314 KKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGFMAQDIWFQNTAGPAKHQAV 373

Query: 377 ALRVGSDFSAFYRCDMLAYQDTLYVHSNRQFYVSCIIIGTVDFIFGNAAAVIQDCDIHAR 436
           ALRV +D +   RC + AYQDTLY H+ RQFY    I GTVDFIFGN+A V Q+CDI AR
Sbjct: 374 ALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTVDFIFGNSAVVFQNCDIVAR 433

Query: 437 RPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRTVV 496
            P  GQ+NM+TAQGR D NQNT I IQKC+I  +SDL PV  +  TFLGRPW+ YSRTV+
Sbjct: 434 NPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVKGSVKTFLGRPWKLYSRTVI 493

Query: 497 MQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGPGADTSRRVTWKGYRVLTSAAEAQE 556
           MQ+ I N IDPAGW  WDG FAL TL+Y EY N+GPGADTS+RV WKG++V+  + EA++
Sbjct: 494 MQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTSKRVNWKGFKVIKDSKEAEQ 553

Query: 557 FTAGNFIGGGSWLSSTGFPF 563
           FT    I GG WL  TG  F
Sbjct: 554 FTVAKLIQGGLWLKPTGVTF 553

BLAST of Lag0016880 vs. TAIR 10
Match: AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 407.1 bits (1045), Expect = 2.2e-113
Identity = 225/481 (46.78%), Postives = 293/481 (60.91%), Query Frame = 0

Query: 94  AVKRNYKSIKKLVATRKNLSK-----REKIALEDCLENGEETLRELYEVVE-DLNEYPKK 153
           A K    +I K VA      K     R   A+ DC++  +    EL  ++    +   K 
Sbjct: 50  AAKTVVDAITKAVAIVSKFDKKAGKSRVSNAIVDCVDLLDSAAEELSWIISASQSPNGKD 109

Query: 154 KALSQYADDLKTLLSSAITNQETCLDGFSHDKADKIVRKSLESGQIHVEKLCSIALALIK 213
            +      DL+T +S+A++NQ+TCLDGF  +  + I++K +  G   + K+ +    L+ 
Sbjct: 110 NSTGDVGSDLRTWISAALSNQDTCLDGF--EGTNGIIKKIVAGG---LSKVGTTVRNLLT 169

Query: 214 NLTDTDIANRNNNLVRKQLTEDDG--IQWPDWLPAGDRRLLQASSAATPDAVVAADGSGD 273
            +       +   +  + +T+      ++P W+  GDR+LLQ  +    DAVVAADG+G+
Sbjct: 170 MVHSPPSKPKPKPIKAQTMTKAHSGFSKFPSWVKPGDRKLLQTDNITVADAVVAADGTGN 229

Query: 274 FTTVSAAVAAAPNGSSRRYIIRIKAGVYRENVNVASGKRNIMFWGDGRTTTIITGNRNVV 333
           FTT+S AV AAP+ S++RY+I +K GVY ENV +   K NIM  GDG   T+ITGNR+ +
Sbjct: 230 FTTISDAVLAAPDYSTKRYVIHVKRGVYVENVEIKKKKWNIMMVGDGIDATVITGNRSFI 289

Query: 334 DGSTTFNSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYRCDMLAYQDTL 393
           DG TTF SAT A  G  F+ARDITFQNTAGP KHQAVA+R  +D   FYRC M  YQDTL
Sbjct: 290 DGWTTFRSATFAVSGRGFIARDITFQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTL 349

Query: 394 YVHSNRQFYVSCIIIGTVDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTG 453
           Y HS RQF+  CII GTVDFIFG+A AV Q C I A++  P Q+N +TAQGR DPN+ TG
Sbjct: 350 YAHSMRQFFRECIITGTVDFIFGDATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTG 409

Query: 454 IVIQKCRIGTTSDLQPVIRNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFAL 513
             IQ   I   +DL   +    T+LGRPW+ YSRTV MQ  +S+ I+P GW  W+GNFAL
Sbjct: 410 FTIQFSNIAADTDLLLNLNTTATYLGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFAL 469

Query: 514 DTLFYAEYQNSGPGADTSRRVTWKGYRVLTSAAEAQEFTAGNFIGGGSWLSSTGFPFSLG 567
           DTL+Y EY NSGPGA   RRV W GY VL ++AEA  FT    I G  WL STG  F  G
Sbjct: 470 DTLYYGEYMNSGPGASLDRRVKWPGYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAG 525

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023526589.13.7e-26784.24pectinesterase-like [Cucurbita pepo subsp. pepo][more]
XP_022980581.14.1e-26683.48pectinesterase-like [Cucurbita maxima][more]
KAG6581794.11.2e-26583.71Pectinesterase/pectinesterase inhibitor U1, partial [Cucurbita argyrosperma subs... [more]
XP_022955430.13.4e-26583.36pectinesterase-like [Cucurbita moschata][more]
KAG7018245.14.5e-26583.54Pectinesterase/pectinesterase inhibitor U1 [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
O048861.6e-21268.37Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
P839481.0e-21168.08Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
Q431433.3e-21065.87Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O490061.2e-20966.84Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425343.0e-20364.54Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1ITZ42.0e-26683.48Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1[more]
A0A6J1GV421.7e-26583.36Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1[more]
A0A5A7UEV09.8e-25881.82Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00109... [more]
A0A1S3CCA59.8e-25881.82Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1[more]
A0A0A0LCR61.3e-25781.22Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.18.9e-21166.84pectin methylesterase 3 [more]
AT1G53830.12.1e-20464.54pectin methylesterase 2 [more]
AT2G45220.11.2e-11745.96Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G11580.15.4e-11543.57methylesterase PCR A [more]
AT3G43270.12.2e-11346.78Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 124..144
NoneNo IPR availablePANTHERPTHR31707:SF180PECTINESTERASEcoord: 39..565
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 39..565
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 51..210
e-value: 1.46804E-38
score: 136.799
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 44..205
e-value: 5.6E-36
score: 135.5
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 48..205
e-value: 1.1E-27
score: 97.2
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 23..210
e-value: 2.5E-25
score: 86.8
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 46..211
e-value: 1.3E-31
score: 111.6
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 48..210
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 249..566
e-value: 1.0E-126
score: 424.7
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 255..551
e-value: 1.5E-144
score: 480.2
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 399..408
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 280..299
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 249..565

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016880.1Lag0016880.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity