Lag0016852 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016852
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionUbiquitin-like protein ATG12
Locationchr12: 41864426 .. 41866833 (+)
RNA-Seq ExpressionLag0016852
SyntenyLag0016852
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTTCTATGGAGTCGTCTAGTTCTGCTCCAAAAGGTGAGCTTCTTTGACGATGTATGAAAAAACCCATCTTCAATTTGAGCTAATTTAGCTGTATTTTGATGAATCGAACTTTCAAACGTTGTTCAATCATCGGGCTTAGTTGATCTGAGTCAGAAGAAGAATTGTTTTACAATTGCTGTTTGATTACGACGCTTTCCTAATGTGGTTTTCGATCAAGAATTATGGTGATTTATTTGGTTTTGTTTTTGGTAGTGGTTGTTCTGCTGAGAGCTACTGGGGATGCTCCCATTCTCAAACAAACCAAGTTTAAGGTTTGTATTACGTTAAATTTGTGTGTTATGTCGTTGAACATGTAAAAATAGAAATCCAATCTTGATGCCTATGAAAAATTTTAATCATTTGATTGGATCAAGATAAACTGAACAAGAACCGACTGATTGTAGTTAGTATCAAGAATCAAACCGATTGATTTGTTGAAACACCACTAAATCCAAAAGCTTAAACTGATAGGGTATGATAAATTTAGTGTTTTTATCAATACTTTAACATGATTCTGGCAATGGTGTATGTCTATGTAGGTAAGTTTGTGCACACACAACCATTGTTTTAAAGCTTGAGGTTCACTAAAGCGCATCAGCCCTCAGAGGCACGTTTTTTAGTTAATATTAAGAGCGAGACACAAATAAAAGCGGGGAGTTTTTTTATAAAACGTTCAAGACGCACAAGCTGGCCACCTACCTAGGATTTAATAATGGTCATGGGAAAAATAAAGGGCTTAGAGGGAATAAGTTCAATTCATGATGACCACCTACCTAGGATTTAATATCCTATGTGTTTCTTGGCAACCAAATGTAGTATGGTTAGGTGGTTGTCTCGTGAGATTAGTCGAGGTGCGTACAACCTGGCTCAAACACTCACGGATATTAAAAAAATAAAGAGTTTAAGACGCACATCAATTGTTGAGATGCGAGCCTCAAGCGGGCTTTTTAAAACACTGTGCGCACACACACACAGATAATAGTTGCTGAGTGAGACATAGATGAAGTAGGCTAATAGATGAAGAAGACAACTAATCTAAGAGCCAAAAGGAATGAAGCTTAATCTTTGTCTCTTTTTTTTTTTTTTTTTTGAATTGTTTCATATGTATTAGATCTGATGGATGCAATGCCACATCCTTATGATGTACAAAATTTACATCGCATTGAGGAAATTTTCATAGTATAACTGATGAATTAAATAGTTCTAAAAAAAAATGATGAATTAAATAATATGGTTTTTTTTTTTTTTTTTTTTGGGGGGGGGGGGGGTAAACTTATGAAATGGCCAATATTAGAATGTGTGGGTTGTGCAAAATTGTATACAGAAGCTCCTCCCCTCAAAGTTTCTCTGTTTGGAATTTGGTTTACTATATCTGTAAGTTTATGACAAATGTTCATTCACATATTGCATACATTTGGCAGATGCCTGGAACAGACAAATTTATTAAAGTTATTGATTACATACGTCGGTCAATTCAACGAGACACCTTGGTATTCATCTGAGTTCAGAAATAGTCTCTCCTGTTTGCCAGTTCATTTGAAATTAATGTCCCTTCACTATGCTAATTGTTTCTATTCTTTGAAGTTTGTGTTTGTCAACAGTGCATTCTCACCGGGTCCAGATGAGACAGTTATTGATCTATATAATGTGAGTTGATATTGACTCATTTCGTCAATTTATATTCAAGTTGTGTTTGTGTACTGTTTTCACTAATTTCTTGAAGAAGAAAAGGAGGTGGGGGAATTTCTTTTCTGATGAATTTGTTGGAAAATACCTTAAAAGGCTCTATTGGCTAGTTTAAAACTGTCATCTTAAGTGAGGGGTAGATTGAAATTTGAAGATGTAGACTTCTGATGTTGATGTTGGTCATGAAATTTCATGAAAGCACAGATCATTTTATATTCTTAGAAGGTGAAAGAAAAGGTCTAGCTGAGGTGGCATGTACCAGTACGGCATTGCTTACTTTTGGTGGTTGAATGATTCTGGGAAGAGTATAGATCTACATTCAAATCGGATAATTAATTTGAGAACTTTCTGAAGCATGTTAACCACAAGATCTGTTAGATTATCTGAAAATATAGCATCATATGAGTTCACTTCAATGTTTTAAACATCTTTTTTTAATCACCTTTACCTGATGGATGTGTAAGGGTAAAATCTTTCTGACTTTCATAGTTGTTTCTCAATCTGTTTCTTTACCTAAGGATAAATATATGGTTTTGCATATCCACATGAGTTTAGAGTTTTCTCAAGTCCTGGTGGCTAGGAATCTTACTGATTATCTTTTTCGTGTGCCTATTTGCAGAACTTTGGAATTGACGGGAAGTTGGTGGTAAATTATGCCTGCTCCATGGCTTGGGGCTAA

mRNA sequence

ATGGCTTCTATGGAGTCGTCTAGTTCTGCTCCAAAAGTGGTTGTTCTGCTGAGAGCTACTGGGGATGCTCCCATTCTCAAACAAACCAAGTTTAAGATGCCTGGAACAGACAAATTTATTAAAGTTATTGATTACATACGTCGGTCAATTCAACGAGACACCTTGTTTGTGTTTGTCAACAGTGCATTCTCACCGGGTCCAGATGAGACAGTTATTGATCTATATAATAACTTTGGAATTGACGGGAAGTTGGTGGTAAATTATGCCTGCTCCATGGCTTGGGGCTAA

Coding sequence (CDS)

ATGGCTTCTATGGAGTCGTCTAGTTCTGCTCCAAAAGTGGTTGTTCTGCTGAGAGCTACTGGGGATGCTCCCATTCTCAAACAAACCAAGTTTAAGATGCCTGGAACAGACAAATTTATTAAAGTTATTGATTACATACGTCGGTCAATTCAACGAGACACCTTGTTTGTGTTTGTCAACAGTGCATTCTCACCGGGTCCAGATGAGACAGTTATTGATCTATATAATAACTTTGGAATTGACGGGAAGTTGGTGGTAAATTATGCCTGCTCCATGGCTTGGGGCTAA

Protein sequence

MASMESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG
Homology
[Close]
BLAST of Lag0016852 vs. NCBI nr
Match: XP_038878493.1 (ubiquitin-like protein ATG12 [Benincasa hispida])

HSP 1 Score: 186.0 bits (471), Expect = 1.5e-43
Identity = 93/95 (97.89%), Postives = 93/95 (97.89%), Query Frame = 0 

Query: 1  MASMESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60
          MAS ESSSSA KVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN
Sbjct: 1  MASTESSSSARKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60

Query: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG
Sbjct: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 95

[Close]
BLAST of Lag0016852 vs. NCBI nr
Match: XP_004151012.1 (ubiquitin-like protein ATG12 [Cucumis sativus] >KGN59786.1 hypothetical protein Csa_001664 [Cucumis sativus])

HSP 1 Score: 184.5 bits (467), Expect = 4.3e-43
Identity = 92/95 (96.84%), Postives = 92/95 (96.84%), Query Frame = 0 

Query: 1  MASMESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60
          M S ESSSSA KVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN
Sbjct: 1  MTSTESSSSARKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60

Query: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG
Sbjct: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 95

[Close]
BLAST of Lag0016852 vs. NCBI nr
Match: XP_008464001.1 (PREDICTED: ubiquitin-like protein ATG12 [Cucumis melo])

HSP 1 Score: 183.3 bits (464), Expect = 9.5e-43
Identity = 91/95 (95.79%), Postives = 92/95 (96.84%), Query Frame = 0 

Query: 1  MASMESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60
          M S ESS+SA KVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN
Sbjct: 1  MTSTESSTSARKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60

Query: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG
Sbjct: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 95

[Close]
BLAST of Lag0016852 vs. NCBI nr
Match: XP_022955552.1 (ubiquitin-like protein ATG12 [Cucurbita moschata] >XP_023526053.1 ubiquitin-like protein ATG12 isoform X1 [Cucurbita pepo subsp. pepo] >KAG6581816.1 Ubiquitin-like protein ATG12, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018264.1 Ubiquitin-like protein ATG12 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 183.3 bits (464), Expect = 9.5e-43
Identity = 91/95 (95.79%), Postives = 92/95 (96.84%), Query Frame = 0 

Query: 1  MASMESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60
          MASMESSSSA KVVVLLR+ GDAPILKQ KFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN
Sbjct: 1  MASMESSSSAKKVVVLLRSIGDAPILKQNKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60

Query: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG
Sbjct: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 95

[Close]
BLAST of Lag0016852 vs. NCBI nr
Match: XP_022980759.1 (ubiquitin-like protein ATG12 [Cucurbita maxima])

HSP 1 Score: 181.0 bits (458), Expect = 4.7e-42
Identity = 89/95 (93.68%), Postives = 92/95 (96.84%), Query Frame = 0 

Query: 1  MASMESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60
          MASMESS+S+ KVVVLLR+ GDAPILKQ KFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN
Sbjct: 1  MASMESSNSSKKVVVLLRSIGDAPILKQNKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60

Query: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG
Sbjct: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 95

[Close]
BLAST of Lag0016852 vs. ExPASy Swiss-Prot
Match: Q1SF86 (Ubiquitin-like protein ATG12 OS=Medicago truncatula OX=3880 GN=ATG12 PE=3 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 2.4e-36
Identity = 73/95 (76.84%), Postives = 83/95 (87.37%), Query Frame = 0 

Query: 1  MASMESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVN 60
          MA+ ES +S  KVVV LRATGDAPILKQ+KFK+ GTDKF KVID++RR + R++LFV+VN
Sbjct: 1  MAAAESPTSVRKVVVHLRATGDAPILKQSKFKIAGTDKFAKVIDFLRRQLHRESLFVYVN 60

Query: 61 SAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          SAFSP PDE VIDLYNNFG DGKLVVNYACSMAWG
Sbjct: 61 SAFSPNPDELVIDLYNNFGFDGKLVVNYACSMAWG 95

[Close]
BLAST of Lag0016852 vs. ExPASy Swiss-Prot
Match: Q8S924 (Ubiquitin-like protein ATG12A OS=Arabidopsis thaliana OX=3702 GN=ATG12A PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 2.6e-35
Identity = 74/96 (77.08%), Postives = 83/96 (86.46%), Query Frame = 0 

Query: 1  MASMESS-SSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFV 60
          MA+  SS SS  KVVV LRATG APILKQ+KFK+PGTDKF KVID++RR +  D+LFV+V
Sbjct: 1  MATESSSPSSVRKVVVHLRATGGAPILKQSKFKIPGTDKFAKVIDFLRRQLHSDSLFVYV 60

Query: 61 NSAFSPGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          NSAFSP PDE+VIDLYNNFG DGKLVVNYACSMAWG
Sbjct: 61 NSAFSPNPDESVIDLYNNFGFDGKLVVNYACSMAWG 96

[Close]
BLAST of Lag0016852 vs. ExPASy Swiss-Prot
Match: Q9LVK3 (Ubiquitin-like protein ATG12B OS=Arabidopsis thaliana OX=3702 GN=ATG12B PE=1 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 1.4e-33
Identity = 67/91 (73.63%), Postives = 78/91 (85.71%), Query Frame = 0 

Query: 5  ESSSSAPKVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFS 64
          ES +S  K+VV LRATG APILKQ+KFK+ G+DKF  VID++RR +  D+LFV+VNSAFS
Sbjct: 4  ESPNSVQKIVVHLRATGGAPILKQSKFKVSGSDKFANVIDFLRRQLHSDSLFVYVNSAFS 63

Query: 65 PGPDETVIDLYNNFGIDGKLVVNYACSMAWG 96
          P PDE+VIDLYNNFG DGKLVVNYACSMAWG
Sbjct: 64 PNPDESVIDLYNNFGFDGKLVVNYACSMAWG 94

[Close]
BLAST of Lag0016852 vs. ExPASy Swiss-Prot
Match: A2YAG8 (Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. indica OX=39946 GN=ATG12 PE=3 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 5.6e-30
Identity = 59/84 (70.24%), Postives = 74/84 (88.10%), Query Frame = 0 

Query: 12 KVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV 71
          KVVV  R+TG+AP LKQ+KFK+ G +KF+K+ID++RR I +DT+F++VNSAFSP PDE +
Sbjct: 10 KVVVHFRSTGNAPQLKQSKFKIGGNEKFLKIIDFLRRQIHQDTVFLYVNSAFSPNPDELI 69

Query: 72 IDLYNNFGIDGKLVVNYACSMAWG 96
          IDLYNNFGIDG+LVVNYA SMAWG
Sbjct: 70 IDLYNNFGIDGQLVVNYASSMAWG 93

[Close]
BLAST of Lag0016852 vs. ExPASy Swiss-Prot
Match: Q69NP0 (Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. japonica OX=39947 GN=ATG12 PE=3 SV=2)

HSP 1 Score: 131.3 bits (329), Expect = 5.6e-30
Identity = 59/84 (70.24%), Postives = 74/84 (88.10%), Query Frame = 0 

Query: 12 KVVVLLRATGDAPILKQTKFKMPGTDKFIKVIDYIRRSIQRDTLFVFVNSAFSPGPDETV 71
          KVVV  R+TG+AP LKQ+KFK+ G +KF+K+ID++RR I +DT+F++VNSAFSP PDE +
Sbjct: 10 KVVVHFRSTGNAPQLKQSKFKIGGNEKFLKIIDFLRRQIHQDTVFLYVNSAFSPNPDELI 69

Query: 72 IDLYNNFGIDGKLVVNYACSMAWG 96
          IDLYNNFGIDG+LVVNYA SMAWG
Sbjct: 70 IDLYNNFGIDGQLVVNYASSMAWG 93

The following BLAST results are available for this feature:
BLAST of Lag0016852 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038878493.11.5e-4397.89ubiquitin-like protein ATG12 [Benincasa hispida][more]
XP_004151012.14.3e-4396.84ubiquitin-like protein ATG12 [Cucumis sativus] >KGN59786.1 hypothetical protein ... [more]
XP_008464001.19.5e-4395.79PREDICTED: ubiquitin-like protein ATG12 [Cucumis melo][more]
XP_022955552.19.5e-4395.79ubiquitin-like protein ATG12 [Cucurbita moschata] >XP_023526053.1 ubiquitin-like... [more]
XP_022980759.14.7e-4293.68ubiquitin-like protein ATG12 [Cucurbita maxima][more]
BLAST of Lag0016852 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q1SF862.4e-3676.84Ubiquitin-like protein ATG12 OS=Medicago truncatula OX=3880 GN=ATG12 PE=3 SV=1[more]
Q8S9242.6e-3577.08Ubiquitin-like protein ATG12A OS=Arabidopsis thaliana OX=3702 GN=ATG12A PE=1 SV=... [more]
Q9LVK31.4e-3373.63Ubiquitin-like protein ATG12B OS=Arabidopsis thaliana OX=3702 GN=ATG12B PE=1 SV=... [more]
A2YAG85.6e-3070.24Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. indica OX=39946 GN=ATG12 PE=... [more]
Q69NP05.6e-3070.24Ubiquitin-like protein ATG12 OS=Oryza sativa subsp. japonica OX=39947 GN=ATG12 P... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007242Ubiquitin-like protein Atg12PFAMPF04110APG12coord: 12..95
e-value: 8.6E-30
score: 103.0
IPR007242Ubiquitin-like protein Atg12PANTHERPTHR13385AUTOPHAGY PROTEIN 12coord: 4..95
NoneNo IPR availableGENE3D3.10.20.90coord: 6..95
e-value: 6.5E-35
score: 121.6
NoneNo IPR availablePANTHERPTHR13385:SF2UBIQUITIN-LIKE PROTEIN ATG12Bcoord: 4..95
NoneNo IPR availableCDDcd01612Ubl_ATG12coord: 12..94
e-value: 1.53014E-40
score: 126.863
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 12..94

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016852.1Lag0016852.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000045 autophagosome assembly
biological_process GO:0000422 autophagy of mitochondrion
biological_process GO:0044804 autophagy of nucleus
biological_process GO:0006501 C-terminal protein lipidation
cellular_component GO:0034274 Atg12-Atg5-Atg16 complex
cellular_component GO:0034045 phagophore assembly site membrane
cellular_component GO:0005737 cytoplasm