Lag0016812 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0016812
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionPectinesterase inhibitor
Locationchr12: 41499612 .. 41500184 (+)
RNA-Seq ExpressionLag0016812
SyntenyLag0016812
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTCATCACCTATTCTTGTTGTTATGATCTTGCTCGTTTTCGCTGTAGAAGCCAATATTATTGATTCAGAGACGAAGGTGGCGAATGATTTTGTTCGAGATACATGCAACAAAACCGAAAAGAAAGATCTTTGCATTTCCGTCGTGAATTCAGATCCTCGTGAAGATTTGAAATCAAACTTGACAGGTCTTCTCATCATATTCACTAACCAAACTTTCTATGCTGTGGTTAATGCTCATACTTACATTTACAATCAATTAAAAGATGGACATTTGAGGAATCGAACGCAACAAGTCTTTATAGGATGTTTGGAGCAATATCGAATGAGCCAAGAAACTTTGAGATATTTATTAGAGGAAAAACTATTGAAGCAAAAGAATACAGATTTGAATTCAGATTTGTCAATGGTTGATAGCAAGTTAGGCCTTTGTGAAGATGATTTTGAGGGGTTTACACAAGAACCATCGACTTTGAAGTCTCTCTATGATCATGCTTGGTCACTACTACTGCTTTCATTGGATATGACAAATATCATCAAATGTGGTCGTTTTGACCAACCTGTTTGCTAG

mRNA sequence

ATGAATTCATCACCTATTCTTGTTGTTATGATCTTGCTCGTTTTCGCTGTAGAAGCCAATATTATTGATTCAGAGACGAAGGTGGCGAATGATTTTGTTCGAGATACATGCAACAAAACCGAAAAGAAAGATCTTTGCATTTCCGTCGTGAATTCAGATCCTCGTGAAGATTTGAAATCAAACTTGACAGGTCTTCTCATCATATTCACTAACCAAACTTTCTATGCTGTGGTTAATGCTCATACTTACATTTACAATCAATTAAAAGATGGACATTTGAGGAATCGAACGCAACAAGTCTTTATAGGATGTTTGGAGCAATATCGAATGAGCCAAGAAACTTTGAGATATTTATTAGAGGAAAAACTATTGAAGCAAAAGAATACAGATTTGAATTCAGATTTGTCAATGGTTGATAGCAAGTTAGGCCTTTGTGAAGATGATTTTGAGGGGTTTACACAAGAACCATCGACTTTGAAGTCTCTCTATGATCATGCTTGGTCACTACTACTGCTTTCATTGGATATGACAAATATCATCAAATGTGGTCGTTTTGACCAACCTGTTTGCTAG

Coding sequence (CDS)

ATGAATTCATCACCTATTCTTGTTGTTATGATCTTGCTCGTTTTCGCTGTAGAAGCCAATATTATTGATTCAGAGACGAAGGTGGCGAATGATTTTGTTCGAGATACATGCAACAAAACCGAAAAGAAAGATCTTTGCATTTCCGTCGTGAATTCAGATCCTCGTGAAGATTTGAAATCAAACTTGACAGGTCTTCTCATCATATTCACTAACCAAACTTTCTATGCTGTGGTTAATGCTCATACTTACATTTACAATCAATTAAAAGATGGACATTTGAGGAATCGAACGCAACAAGTCTTTATAGGATGTTTGGAGCAATATCGAATGAGCCAAGAAACTTTGAGATATTTATTAGAGGAAAAACTATTGAAGCAAAAGAATACAGATTTGAATTCAGATTTGTCAATGGTTGATAGCAAGTTAGGCCTTTGTGAAGATGATTTTGAGGGGTTTACACAAGAACCATCGACTTTGAAGTCTCTCTATGATCATGCTTGGTCACTACTACTGCTTTCATTGGATATGACAAATATCATCAAATGTGGTCGTTTTGACCAACCTGTTTGCTAG

Protein sequence

MNSSPILVVMILLVFAVEANIIDSETKVANDFVRDTCNKTEKKDLCISVVNSDPREDLKSNLTGLLIIFTNQTFYAVVNAHTYIYNQLKDGHLRNRTQQVFIGCLEQYRMSQETLRYLLEEKLLKQKNTDLNSDLSMVDSKLGLCEDDFEGFTQEPSTLKSLYDHAWSLLLLSLDMTNIIKCGRFDQPVC
Homology
BLAST of Lag0016812 vs. NCBI nr
Match: KAG6599042.1 (hypothetical protein SDJN03_08820, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 203.0 bits (515), Expect = 2.3e-48
Identity = 110/184 (59.78%), Postives = 139/184 (75.54%), Query Frame = 0

Query: 1   MNSSPILVVMI-LLVFAVEANIIDSETKVANDFVRDTCNKTEK-KDLCISVVNSDPREDL 60
           MN S ++VV++ +LV  V ANI+D E +VANDFV DTCNKTE+ KDLC SV+ SDPR+DL
Sbjct: 1   MNQSIVVVVLVSVLVSFVAANILDLEMEVANDFVHDTCNKTEELKDLCNSVIGSDPRDDL 60

Query: 61  KSNLTGLLIIFTNQTFYAVVNAHTYIYNQLKDGHLRNRTQQVFIGCLEQYRMSQETLRYL 120
           K+NLT LL IF NQT   VV+ H+Y+YNQ  +G L NRT +VF  C+  Y+MS+++L+ L
Sbjct: 61  KTNLTSLLTIFINQTLRVVVDDHSYLYNQTLNGELDNRTLEVFKSCMNLYQMSKDSLQAL 120

Query: 121 LEEKLLKQKNTDLNSDLSMVDSKLGLCEDDFEGFTQEPSTLKSLYDHAWSLLLLSLDMTN 180
           L+ +LLK KN DLN DL+ VD+ L LCE DFEGFT E  T KS YD+  SLL+LSL MTN
Sbjct: 121 LDVELLKNKNADLNMDLAQVDNFLDLCEVDFEGFTPESLTWKSRYDYVSSLLILSLRMTN 180

Query: 181 IIKC 183
           +IKC
Sbjct: 181 LIKC 184

BLAST of Lag0016812 vs. NCBI nr
Match: KAG6585821.1 (hypothetical protein SDJN03_18554, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 192.6 bits (488), Expect = 3.1e-45
Identity = 101/185 (54.59%), Postives = 131/185 (70.81%), Query Frame = 0

Query: 1   MNSSPILVVMILLVFAVEANIIDSETKVANDFVRDTCNKT-EKKDLCISVVNSDPREDLK 60
           MN S  +V    L+ +  AN +D E K  +DF R TC++T E KDLCISV+NS+P EDLK
Sbjct: 1   MNQS-FVVFFTYLLLSAAANTLDPEMKAGSDFTRATCDRTSELKDLCISVINSNPCEDLK 60

Query: 61  SNLTGLLIIFTNQTFYAVVNAHTYIYNQLKDGHLRNRTQQVFIGCLEQYRMSQETLRYLL 120
           SNLTG L IF N+T + V++ H+Y+ NQ  +G L NRT QVF  C  QY+MS+++LR LL
Sbjct: 61  SNLTGFLAIFINETLHVVIDDHSYLLNQTLNGQLDNRTLQVFRSCASQYQMSKDSLRVLL 120

Query: 121 EEKLLKQKNTDLNSDLSMVDSKLGLCEDDFEGFTQEPSTLKSLYDHAWSLLLLSLDMTNI 180
           E++LLKQ N DLN DL  +D+ L LCE +FEGF +EPS  K+ YD+  SLL+LSL  TN+
Sbjct: 121 EDELLKQHNADLNMDLGQIDNYLALCEMEFEGFIEEPSAWKTRYDYVSSLLILSLQTTNL 180

Query: 181 IKCGR 185
           IKC R
Sbjct: 181 IKCNR 184

BLAST of Lag0016812 vs. NCBI nr
Match: KAG6599036.1 (hypothetical protein SDJN03_08814, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 172.6 bits (436), Expect = 3.4e-39
Identity = 84/157 (53.50%), Postives = 113/157 (71.97%), Query Frame = 0

Query: 27  KVANDFVRDTCNKTEK-KDLCISVVNSDPREDLKSNLTGLLIIFTNQTFYAVVNAHTYIY 86
           K  NDF++DTCN+TE  KDLCISV++SDPREDLK N    L+IF N+T   +++ H+Y+ 
Sbjct: 2   KAPNDFIQDTCNRTEDFKDLCISVIDSDPREDLKLNSMDFLVIFINRTLQVIIDDHSYLL 61

Query: 87  NQLKDGHLRNRTQQVFIGCLEQYRMSQETLRYLLEEKLLKQKNTDLNSDLSMVDSKLGLC 146
           NQ  +  L NRT+ +F+GC+  Y+MS+++LR LL E+LLKQ N DLN  L  ++  L LC
Sbjct: 62  NQTLNEELDNRTRHIFMGCMTLYQMSKDSLRVLLNEELLKQPNADLNMKLQQIEGYLDLC 121

Query: 147 EDDFEGFTQEPSTLKSLYDHAWSLLLLSLDMTNIIKC 183
           ++DFEGFT  PST  S YD+  SLL+LS  M N+IKC
Sbjct: 122 QNDFEGFTPTPSTWTSRYDYVISLLILSFRMMNLIKC 158

BLAST of Lag0016812 vs. NCBI nr
Match: XP_022137307.1 (uncharacterized protein LOC111008804 [Momordica charantia])

HSP 1 Score: 103.2 bits (256), Expect = 2.5e-18
Identity = 66/157 (42.04%), Postives = 87/157 (55.41%), Query Frame = 0

Query: 28  VANDFVRDTCNKTEKK--DLCISVVNSDPREDLKSNLTGLLIIFTNQTFYAVVNAHTYIY 87
           VAN FV +TCNK E++   +CI V++SDP +DLK NL+GLL IF N +     + H Y+ 
Sbjct: 9   VANSFVNNTCNKFEEEFIKVCIDVIDSDPHQDLKLNLSGLLAIFINHSIDNATDNHLYLM 68

Query: 88  NQLKDGHLRNRTQQVFIGCLEQYRMSQETLRYLLEEKLLKQKNTDLNSDLSMVDSKLGLC 147
            QLK   L    +  F  C+EQY    + L+  L E LLK    DL   LS    K+  C
Sbjct: 69  EQLKRSDLDKDIRYTFNVCVEQYERGLDILKSSL-EALLKNDKDDLGLILSDAFDKIVAC 128

Query: 148 EDDFEGFTQEPSTLKSLYDHAWSLLLLSLDMTNIIKC 183
           EDDFE  T EP +  + Y     LL LS+   N+I+C
Sbjct: 129 EDDFES-TPEPPSWLAHYLPLRKLLALSMGTANLIQC 163

BLAST of Lag0016812 vs. NCBI nr
Match: KAA0055284.1 (putative invertase inhibitor [Cucumis melo var. makuwa] >TYJ99207.1 putative invertase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 97.8 bits (242), Expect = 1.1e-16
Identity = 66/188 (35.11%), Postives = 105/188 (55.85%), Query Frame = 0

Query: 1   MNSSPILVVMILLVFAVEANIIDSETKVANDFVRDTCNKT-EKKDLCISVVNSDPREDLK 60
           M S    +V++ ++        D  T + NDFVR TCN+T E K+LC+S ++S+PR DLK
Sbjct: 1   MASDRQFLVLLCILSLCMLTKADQNTTI-NDFVRHTCNRTHELKNLCLSQISSEPRNDLK 60

Query: 61  SNLTGLLIIFTNQTFYAVVNAHTYIYNQLKDGHLRNRTQQVFIGCLEQYRMSQETLRYLL 120
           SNLTGLL+IF NQ+     N  +++  ++    +   T+ +   CL+ +++    LR  +
Sbjct: 61  SNLTGLLMIFVNQSISNFKNDISFLEKEINSNKISRDTKDMLEDCLQNFQIGSVNLRETM 120

Query: 121 EEKLLKQKNTDLNSDLSMVDSKLGL---CEDDFEGFTQEPSTLKSLYDHAWSLLLLSLDM 180
           E   + Q  T  +++   V +   L   C DDFEG T  P   +S Y+ +++LL LS+ M
Sbjct: 121 E---ILQTKTGYDAEQLPVGNVGNLAVECFDDFEG-TPVPPEWQSRYNASFALLKLSMAM 180

Query: 181 TNIIKCGR 185
            N+I C R
Sbjct: 181 INLITCNR 183

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6599042.12.3e-4859.78hypothetical protein SDJN03_08820, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6585821.13.1e-4554.59hypothetical protein SDJN03_18554, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG6599036.13.4e-3953.50hypothetical protein SDJN03_08814, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022137307.12.5e-1842.04uncharacterized protein LOC111008804 [Momordica charantia][more]
KAA0055284.11.1e-1635.11putative invertase inhibitor [Cucumis melo var. makuwa] >TYJ99207.1 putative inv... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 28..176
e-value: 5.1E-12
score: 55.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 1..161
e-value: 2.7E-19
score: 67.2
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..155
e-value: 1.9E-13
score: 51.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 28..182
e-value: 2.0E-19
score: 72.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..180

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0016812.1Lag0016812.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0004857 enzyme inhibitor activity