Lag0016810 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0016810
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Pectinesterase
Location	chr12: 41473590 .. 41474437 (+)
RNA-Seq Expression	Lag0016810
Synteny	Lag0016810

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCTTTCTTTAAAGCTATAGTCCTTCTTTCTTTCTTGGAGCTTTCATGTTGCCTTGTTTTCAATTCAACCGTGTCAAAAGACGAAACTGGCGATTTTGCTCTGGTAACCGATGCCATTGTTGCTGCACCAAGCTTGAGTTCCAGCCGGTTTTACATCTATGTAAAAGTCGGCACCTACAACAAACATGTAGAGATACCGGGAGACAAAACTTTCATAAGTTTGATCGGAGACGGTGCATCCAACACGGTGATCGTCGACGGTCGAAACAATCACGACGGTTCTTCGACTACTGACTTGACTACAATTAGTAAGCTAGCTAAGCCCTTTTTTTTTATTTAAAAATCTGATTTAGAATATAAATGAAAGTATGTTCAAAGTTGTTTGCTAATTGATTGATAATTAATTTTGCAGCTGTGAAGGGTGAGCATTTTATGGCCCAGTTCATGAGGGTAAGTTGTCGCCTTTCTTGATGAAGCTAACCACAATGCTTATTACAAGTGTGTGTTCAAGGGCTATCAAGACACTCTCTTTGTACGAGGAAGATTTCAATTTTATAAGAACTACAGTGAATATGGAAGTGTCGACTTTATCTTCGGAGAAGGTAGAGTAGTCTTCCAAGACTGTAGTATATATATGCTAGATTGTGGGGGAGCACCATGACCGCACAATCCAAAACCCTAGTACAAGAATGAAGTGATTTTGCATTTCAGAACTGCAACATAATGACATCACCCGAGATGGGGTCAGACAAGGTAACATTGTCTCTTGGACGACCGTGGAGAAGCTACTCTATCGTTATTATTTTGCAATCGTTTCTGGACACTGTAGTTGAACCACAAGGATAG

mRNA sequence

ATGTCTTTCTTTAAAGCTATAGTCCTTCTTTCTTTCTTGGAGCTTTCATGTTGCCTTGTTTTCAATTCAACCGTGTCAAAAGACGAAACTGGCGATTTTGCTCTGGTAACCGATGCCATTGTTGCTGCACCAAGCTTGAGTTCCAGCCGGTTTTACATCTATGTAAAAGTCGGCACCTACAACAAACATGTAGAGATACCGGGAGACAAAACTTTCATAAGTTTGATCGGAGACGGTGCATCCAACACGGTGATCGTCGACGGTCGAAACAATCACGACGGTTCTTCGACTACTGACTTGACTACAATTACTGTGAAGGGTGAGCATTTTATGGCCCAGTTCATGAGGAACTGCAACATAATGACATCACCCGAGATGGGGTCAGACAAGGTAACATTGTCTCTTGGACGACCGTGGAGAAGCTACTCTATCGTTATTATTTTGCAATCGTTTCTGGACACTGTAGTTGAACCACAAGGATAG

Coding sequence (CDS)

ATGTCTTTCTTTAAAGCTATAGTCCTTCTTTCTTTCTTGGAGCTTTCATGTTGCCTTGTTTTCAATTCAACCGTGTCAAAAGACGAAACTGGCGATTTTGCTCTGGTAACCGATGCCATTGTTGCTGCACCAAGCTTGAGTTCCAGCCGGTTTTACATCTATGTAAAAGTCGGCACCTACAACAAACATGTAGAGATACCGGGAGACAAAACTTTCATAAGTTTGATCGGAGACGGTGCATCCAACACGGTGATCGTCGACGGTCGAAACAATCACGACGGTTCTTCGACTACTGACTTGACTACAATTACTGTGAAGGGTGAGCATTTTATGGCCCAGTTCATGAGGAACTGCAACATAATGACATCACCCGAGATGGGGTCAGACAAGGTAACATTGTCTCTTGGACGACCGTGGAGAAGCTACTCTATCGTTATTATTTTGCAATCGTTTCTGGACACTGTAGTTGAACCACAAGGATAG

Protein sequence

MSFFKAIVLLSFLELSCCLVFNSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTYNKHVEIPGDKTFISLIGDGASNTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQFMRNCNIMTSPEMGSDKVTLSLGRPWRSYSIVIILQSFLDTVVEPQG

Homology

[Close]

BLAST of Lag0016810 vs. NCBI nr
Match: XP_022148123.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 142.5 bits (358), Expect = 3.1e-30
Identity = 96/261 (36.78%), Postives = 124/261 (47.51%), Query Frame = 0


Query: 1   MSFFKAIV--LLSFLELSCCLV-FNSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKV 60
           +S+F  +V  LL  +E S CL+ FN+TVS D +GD+  ++DAI AAP+  S RFYI+VK 
Sbjct: 6   LSYFMWLVFLLLHSVEFSHCLITFNATVSLDGSGDYVHISDAIAAAPNNGSIRFYIHVKP 65

Query: 61  GTYNKHVEIPGDKTFISLIGDGASNTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQFM-- 120
           G Y +H+E+P  KTFISLIGD AS TVIVD R+NH G STT   T TVK +HF+AQF+  
Sbjct: 66  GIYKEHIEVPELKTFISLIGDDASTTVIVDDRSNHAGFSTTSSATFTVKAKHFVAQFLTF 125

Query: 121 ------------------------------------------------------------ 161
                                                                       
Sbjct: 126 QNSAGPENGQAVAVLSEANYTAYYKCVFMGYQDTLYVRGAFQLFKECDVYGSIDFIFGDG 185

[Close]

BLAST of Lag0016810 vs. NCBI nr
Match: XP_023002625.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita maxima])

HSP 1 Score: 140.2 bits (352), Expect = 1.6e-29
Identity = 90/260 (34.62%), Postives = 121/260 (46.54%), Query Frame = 0


Query: 1   MSFFKAIVLLSFLELSCCLVFNSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTY 60
           M F  +I+LL FL+ S    FNSTVS D TG+F  ++DAIVAAP+ +++RFYI++K G Y
Sbjct: 7   MFFTLSILLLHFLKFSSTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NKHVEIPGDKTFISLIGDGASNTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQFM----- 120
           N+H+EIP  KTFISLIGD AS T+IVD R+   G ST +  T+TV G +FMA+F+     
Sbjct: 67  NEHIEIPSQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNS 126

Query: 121 ------------------------------------------------------------ 161
                                                                       
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

[Close]

BLAST of Lag0016810 vs. NCBI nr
Match: XP_022150332.1 (probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia])

HSP 1 Score: 140.2 bits (352), Expect = 1.6e-29
Identity = 87/254 (34.25%), Postives = 119/254 (46.85%), Query Frame = 0


Query: 7   IVLLSFLELSCCLVFNSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTYNKHVEI 66
           +V+  F++ S C +FN+TVS D +G+F  V+DAI AAP+ S++RFYI+VK G Y +HVE+
Sbjct: 15  VVINHFVKFSYCFMFNATVSPDGSGNFVRVSDAIAAAPNFSTTRFYIHVKPGIYKEHVEV 74

Query: 67  PGDKTFISLIGDGASNTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQFM----------- 126
           P  KTFI+LIG+ AS T+IVD R+N  G ST    T+ VKG++FMAQF+           
Sbjct: 75  PVQKTFIALIGEDASTTIIVDNRSNGSGFSTQTTATLIVKGQNFMAQFLTFENSAEQKEG 134

Query: 127 ------------------------------------------------------------ 161
                                                                       
Sbjct: 135 QAIAVMDQAKHTAYYKCVFLSYQDTLYAGAVPQFFKECDIYGRIDFIFGNGLVVFQDCNI 194

[Close]

BLAST of Lag0016810 vs. NCBI nr
Match: XP_022951717.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita moschata])

HSP 1 Score: 137.9 bits (346), Expect = 7.7e-29
Identity = 89/260 (34.23%), Postives = 122/260 (46.92%), Query Frame = 0


Query: 1   MSFFKAIVLLSFLELSCCLVFNSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTY 60
           M F  +I+LL FL+ S    FNSTVS D TG+F  ++DAIVAAP+ +++RFYI++K G Y
Sbjct: 7   MFFTLSILLLHFLKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NKHVEIPGDKTFISLIGDGASNTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQFM----- 120
           N+H+E+P  KTFISLIGD AS T+IVD R+   G ST++  T+TV G +FMA+F+     
Sbjct: 67  NEHIEVPFQKTFISLIGDNASTTIIVDNRSKAAGFSTSNSATLTVNGANFMAKFLTFQNS 126

Query: 121 ------------------------------------------------------------ 161
                                                                       
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

[Close]

BLAST of Lag0016810 vs. NCBI nr
Match: XP_023538248.1 (probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 137.9 bits (346), Expect = 7.7e-29
Identity = 89/260 (34.23%), Postives = 121/260 (46.54%), Query Frame = 0


Query: 1   MSFFKAIVLLSFLELSCCLVFNSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTY 60
           M F  +I+LL FL+ S    FNSTVS D TG+F  ++DAIVAAP+ +++RFYI++K G Y
Sbjct: 7   MFFTLSILLLHFLKFSNTFDFNSTVSLDGTGNFVKISDAIVAAPNFTTTRFYIHIKPGIY 66

Query: 61  NKHVEIPGDKTFISLIGDGASNTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQFM----- 120
           N+H+E+P  KTFISLIGD AS T+IVD R+   G ST +  T+TV G +FMA+F+     
Sbjct: 67  NEHIEVPFQKTFISLIGDNASTTIIVDNRSKATGFSTANSATLTVNGANFMAKFLTFQNS 126

Query: 121 ------------------------------------------------------------ 161
                                                                       
Sbjct: 127 AGPKMGQAVAVLDQAKHTAYYKCVFLGYQDTLYAAAVPQFFKECDIYGTVDFIFGNGLVV 186

[Close]

BLAST of Lag0016810 vs. ExPASy Swiss-Prot
Match: O81415 (Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3702 GN=PME39 PE=2 SV=1)

HSP 1 Score: 84.3 bits (207), Expect = 1.3e-15
Identity = 42/93 (45.16%), Postives = 61/93 (65.59%), Query Frame = 0


Query: 21  FNSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTYNKHVEIPGDKTFISLIGDGA 80
           +N +V+ D TG+F  + DA+ AAP++S +RF IY+K G Y ++VE+P  KT I  IGDG 
Sbjct: 220 YNLSVAIDGTGNFTTINDAVFAAPNMSETRFIIYIKGGEYFENVELPKKKTMIMFIGDGI 279

Query: 81  SNTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQ 114
             TVI   R+  DG ST    T+ VKG+ ++A+
Sbjct: 280 GKTVIKANRSRIDGWSTFQTPTVGVKGKGYIAK 312

[Close]

BLAST of Lag0016810 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 80.1 bits (196), Expect = 2.5e-14
Identity = 39/92 (42.39%), Postives = 56/92 (60.87%), Query Frame = 0


Query: 22  NSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTYNKHVEIPGDKTFISLIGDGAS 81
           ++TV+ D +GDF  V  A+ AAP  S+ RF I++K G Y ++VE+   KT I  +GDG  
Sbjct: 276 DATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRG 335

Query: 82  NTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQ 114
            T+I   RN  DGS+T    T+   GE F+A+
Sbjct: 336 KTIITGSRNVVDGSTTFHSATVAAVGERFLAR 367

[Close]

BLAST of Lag0016810 vs. ExPASy Swiss-Prot
Match: Q1JPL7 (Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PME18 PE=1 SV=3)

HSP 1 Score: 75.5 bits (184), Expect = 6.2e-13
Identity = 39/92 (42.39%), Postives = 54/92 (58.70%), Query Frame = 0


Query: 22  NSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTYNKHVEIPGDKTFISLIGDGAS 81
           N  V+KD TG F  V +A+ AAP  S++R+ IYVK G Y + ++I   K  + L+GDG  
Sbjct: 246 NVVVAKDGTGKFKTVNEAVAAAPENSNTRYVIYVKKGVYKETIDIGKKKKNLMLVGDGKD 305

Query: 82  NTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQ 114
            T+I    N  DGS+T    T+   G+ FMAQ
Sbjct: 306 ATIITGSLNVIDGSTTFRSATVAANGDGFMAQ 337

[Close]

BLAST of Lag0016810 vs. ExPASy Swiss-Prot
Match: O81301 (Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3702 GN=PME40 PE=2 SV=1)

HSP 1 Score: 75.5 bits (184), Expect = 6.2e-13
Identity = 38/92 (41.30%), Postives = 58/92 (63.04%), Query Frame = 0


Query: 22  NSTVSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTYNKHVEIPGDKTFISLIGDGAS 81
           N  V+++ TG++  + +AI AAP+ S +RF IY+K G Y +++EIP +KT I  IGDG  
Sbjct: 207 NLVVAQNGTGNYTTIGEAISAAPNSSETRFVIYIKCGEYFENIEIPREKTMIMFIGDGIG 266

Query: 82  NTVIVDGRNNHDGSSTTDLTTITVKGEHFMAQ 114
            TVI   R+  DG +     T+ V+G  F+A+
Sbjct: 267 RTVIKANRSYADGWTAFHSATVGVRGSGFIAK 298

[Close]

BLAST of Lag0016810 vs. ExPASy Swiss-Prot
Match: O48711 (Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PME12 PE=2 SV=1)

HSP 1 Score: 75.1 bits (183), Expect = 8.1e-13
Identity = 43/106 (40.57%), Postives = 65/106 (61.32%), Query Frame = 0


Query: 25  VSKDETGDFALVTDAIVAAPSLSSSRFYIYVKVGTYNKHVEIPGDKTFISLIGDGASNTV 84
           V+ D TG+F+ + +AI  AP++S+ R  IYVK G Y+++++IP  KT I LIGDG+  T 
Sbjct: 240 VAADGTGNFSTINEAISFAPNMSNDRVLIYVKEGVYDENIDIPIYKTNIVLIGDGSDVTF 299

Query: 85  IVDGRNNHDGSSTTDLTTITVKGEHFMAQFMRNCNIMTSPEMGSDK 131
           I   R+  DG +T    T+ V GE F+A+     +IM +   G +K
Sbjct: 300 ITGNRSVGDGWTTFRSATLAVSGEGFLAR-----DIMITNTAGPEK 340

The following BLAST results are available for this feature:

BLAST of Lag0016810 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022148123.1	3.1e-30	36.78	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_023002625.1	1.6e-29	34.62	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita maxima]	[more]
XP_022150332.1	1.6e-29	34.25	probable pectinesterase/pectinesterase inhibitor 39 [Momordica charantia]	[more]
XP_022951717.1	7.7e-29	34.23	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita moschata]	[more]
XP_023538248.1	7.7e-29	34.23	probable pectinesterase/pectinesterase inhibitor 39 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0016810 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81415	1.3e-15	45.16	Probable pectinesterase/pectinesterase inhibitor 39 OS=Arabidopsis thaliana OX=3...	[more]
Q42534	2.5e-14	42.39	Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2	[more]
Q1JPL7	6.2e-13	42.39	Pectinesterase/pectinesterase inhibitor 18 OS=Arabidopsis thaliana OX=3702 GN=PM...	[more]
O81301	6.2e-13	41.30	Probable pectinesterase/pectinesterase inhibitor 40 OS=Arabidopsis thaliana OX=3...	[more]
O48711	8.1e-13	40.57	Probable pectinesterase/pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 115..160 e-value: 8.3E-8 score: 31.3 coord: 23..114 e-value: 3.6E-20 score: 71.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 20..114 e-value: 2.1E-22 score: 81.9
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 115..160 e-value: 1.3E-6 score: 30.0
None	No IPR available	PANTHER	PTHR31707:SF147	PECTINESTERASE/PECTINESTERASE INHIBITOR 39-RELATED	coord: 21..114
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 115..160
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 21..114
None	No IPR available	PANTHER	PTHR31707:SF147	PECTINESTERASE/PECTINESTERASE INHIBITOR 39-RELATED	coord: 115..160
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 23..159

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0016810.1	Lag0016810.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0030599	pectinesterase activity