Lag0015492 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0015492 vs. NCBI nr
Match: XP_022139673.1 (uncharacterized protein LOC111010521 [Momordica charantia])

HSP 1 Score: 241.9 bits (616), Expect = 4.3e-60
Identity = 144/249 (57.83%), Postives = 157/249 (63.05%), Query Frame = 0

Query: 1   MSSFMSPVKIDVEKFDGMMNFGLWQVQVKDVLIQSWLHKALKGRPSDNASERL------- 60
           MS FMSPVKIDVEKFDGM+NFGLWQVQVKDVLIQS LHKALKGRPS+ ASE+L       
Sbjct: 295 MSFFMSPVKIDVEKFDGMINFGLWQVQVKDVLIQSELHKALKGRPSEGASEKLSDDGGPM 354

Query: 61  -----------------------------------------------VMVVQWSPVVVL- 120
                                                               W  +  L 
Sbjct: 355 ESSGGSSRGSKKSSMSYEDWEEMDLRAASAIRTSLAKNILANVHRISTAKELWEKLEALY 414

Query: 121 ----------VEEQFYTQRMEEGMKISDHLSVLNGIISELEVIEVKIEDEDKALRLILSL 180
                     ++EQF+T +MEEGMKISDHLS LN II ELE IEVKI+DEDKALRLILSL
Sbjct: 415 QAKGISNRLYLKEQFHTLQMEEGMKISDHLSNLNSIIFELEAIEVKIDDEDKALRLILSL 474

Query: 181 PTSSEHMRPILMYKKETLSFADVTSKLLLEERRLKSEGRTSQEDSVLVASNWKKKKDSVQ 184
           P S EHM+PILMY K+TL+FA+VTSKLL EERRLKSEGRTS EDS LV SNWKKKKDSVQ
Sbjct: 475 PXSYEHMKPILMYGKDTLNFAEVTSKLLSEERRLKSEGRTSHEDSALVVSNWKKKKDSVQ 534

BLAST of Lag0015492 vs. NCBI nr
Match: KAF5765959.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 186.8 bits (473), Expect = 1.7e-43
Identity = 112/227 (49.34%), Postives = 137/227 (60.35%), Query Frame = 0

Query: 6   SPVKIDVEKFDGMMNFGLWQVQVKDVLIQSWLHKALKGRPSDNAS--------------- 65
           SP++ DVEK+DG +NFGLWQVQVKDVLIQS LHKAL+G+P+  +S               
Sbjct: 5   SPMRFDVEKYDGRINFGLWQVQVKDVLIQSGLHKALRGKPTPVSSKDSSGTSKDDDEEWE 64

Query: 66  --------------------------------ERLVMVVQWS--PVVVLVEEQFYTQRME 125
                                           E+L  + Q    P  + ++EQF+T RM+
Sbjct: 65  DLDLRAASAIRLCLAKNVLANVHGISTAKDLWEKLEQLYQGKGIPNRLYLKEQFHTLRMD 124

Query: 126 EGMKISDHLSVLNGIISELEVIEVKIEDEDKALRLILSLPTSSEHMRPILMYKKETLSFA 184
              KISDHLSVLN I+SELE I VK+EDEDKALRLILSL +S EHM+PILMY KETL +A
Sbjct: 125 GDTKISDHLSVLNNIVSELEAIGVKVEDEDKALRLILSLTSSYEHMKPILMYGKETLKYA 184

BLAST of Lag0015492 vs. NCBI nr
Match: KAF5758504.1 (putative RNA-directed DNA polymerase [Helianthus annuus])

HSP 1 Score: 182.6 bits (462), Expect = 3.1e-42
Identity = 110/227 (48.46%), Postives = 136/227 (59.91%), Query Frame = 0

Query: 6   SPVKIDVEKFDGMMNFGLWQVQVKDVLIQSWLHKALKGRPSDNAS--------------- 65
           SP++  VEK+DG +NFGLWQVQVKDVLIQS LHKAL+G+P+  +S               
Sbjct: 5   SPMRFVVEKYDGRINFGLWQVQVKDVLIQSGLHKALRGKPTPVSSKDSSGTSKDDDEEWE 64

Query: 66  --------------------------------ERLVMVVQWSPVV--VLVEEQFYTQRME 125
                                           E+L  + Q   +   + ++EQF+T RM+
Sbjct: 65  DLDLRAASAIRLCLAKNVLANVHGISTAKDLWEKLEQLYQGKGISNRLYLKEQFHTLRMD 124

Query: 126 EGMKISDHLSVLNGIISELEVIEVKIEDEDKALRLILSLPTSSEHMRPILMYKKETLSFA 184
              KISDHLSVLN I+SELE I VK+EDEDKALRLILSL +S EHM+PILMY KETL +A
Sbjct: 125 GDTKISDHLSVLNNIVSELEAIGVKVEDEDKALRLILSLTSSYEHMKPILMYGKETLKYA 184

BLAST of Lag0015492 vs. NCBI nr
Match: KAE8709065.1 (hypothetical protein F3Y22_tig00110332pilonHSYRG00834 [Hibiscus syriacus])

HSP 1 Score: 182.2 bits (461), Expect = 4.1e-42
Identity = 104/205 (50.73%), Postives = 138/205 (67.32%), Query Frame = 0

Query: 9   KIDVEKFDGMMNFGLWQVQVKDVLIQSWLHKALKG------------RPSDNASER---L 68
           + D+EKFDG +NFGLWQVQVKD+LIQS L+KALKG            +PSD++ ++    
Sbjct: 4   RFDIEKFDGRINFGLWQVQVKDILIQSGLYKALKGKPASLSEGKEPDKPSDSSGDKGKSK 63

Query: 69  VMVVQWSPV--------------VVLVEEQFYTQRMEEGMKISDHLSVLNGIISELEVIE 128
           +   +W  +               VL  E+F+  +MEEG KISDHLS LNGI+SELE I 
Sbjct: 64  MSEEEWEELDMRAASQIRLCLAKNVLANEKFHKLQMEEGTKISDHLSALNGIVSELESIG 123

Query: 129 VKIEDEDKALRLILSLPTSSEHMRPILMYKKETLSFADVTSKLLLEERRLKS-EGRTSQE 184
           VKI+DEDKALRLI SLP+S EHM+ +LMY+KE ++F++VTSKL+ EERRLK+ E ++S+ 
Sbjct: 124 VKIDDEDKALRLIWSLPSSYEHMQTVLMYEKENVNFSEVTSKLISEERRLKNGENKSSES 183

BLAST of Lag0015492 vs. NCBI nr
Match: QHN81458.1 (Retrovirus-related Pol polyprotein [Arachis hypogaea])

HSP 1 Score: 180.3 bits (456), Expect = 1.6e-41
Identity = 106/221 (47.96%), Postives = 141/221 (63.80%), Query Frame = 0

Query: 1   MSSFMSPVKIDVEKFDGMMNFGLWQVQVKDVLIQSWLHKALKGRPSDNASE--------- 60
           MS + S VK+++EKFDG +NFGLWQ+QVKDVLIQS LHKALK R S    E         
Sbjct: 1   MSGYSSAVKLEIEKFDGRINFGLWQIQVKDVLIQSGLHKALKERISGMKDEEWEELDLRA 60

Query: 61  ----RLVMV------VQ--------WSPVV-----------VLVEEQFYTQRMEEGMKIS 120
               RL +       VQ        W  +            +L++EQF+  RM+  +KIS
Sbjct: 61  ASAIRLCLAKNVLANVQGIKTAKELWDKLEGLYQSKGISNRLLLKEQFHNLRMDHNVKIS 120

Query: 121 DHLSVLNGIISELEVIEVKIEDEDKALRLILSLPTSSEHMRPILMYKKETLSFADVTSKL 180
           DHLS +NGI+SELE I VKI+DEDKALRLILSLP+S E+++P+LMY KETL+F +V SKL
Sbjct: 121 DHLSAINGIVSELEAIGVKIDDEDKALRLILSLPSSYEYIKPVLMYGKETLNFEEVASKL 180

Query: 181 LLEERRLKSEGRTSQEDSVLVASNWKKKKDSVQKKGCWECG 184
           + EERR+K+EG TS + +++  S+   K +  +   CW+CG
Sbjct: 181 IAEERRMKNEGSTSSDLALVARSDNYGKGNRGRSVTCWKCG 221

BLAST of Lag0015492 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 88.2 bits (217), Expect = 1.1e-16
Identity = 61/194 (31.44%), Postives = 92/194 (47.42%), Query Frame = 0

Query: 5   MSPVKIDVEKFDGMMNFGLWQVQVKDVLIQSWLHKAL----------KGRPSDNASERLV 64
           MS VK +V KF+G   F  WQ +++D+LIQ  LHK L          K     +  ER  
Sbjct: 1   MSGVKYEVAKFNGDNGFSTWQRRMRDLLIQQGLHKVLDVDSKKPDTMKAEDWADLDERAA 60

Query: 65  MVVQ--------------------WSPVVVL-----------VEEQFYTQRMEEGMKISD 124
             ++                    W+ +  L           +++Q Y   M EG     
Sbjct: 61  SAIRLHLSDDVVNNIIDEDTARGIWTRLESLYMSKTLTNKLYLKKQLYALHMSEGTNFLS 120

Query: 125 HLSVLNGIISELEVIEVKIEDEDKALRLILSLPTSSEHMRPILMYKKETLSFADVTSKLL 158
           HL+V NG+I++L  + VKIE+EDKA+ L+ SLP+S +++   +++ K T+   DVTS LL
Sbjct: 121 HLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHGKTTIELKDVTSALL 180