Lag0014878 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0014878
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCalcium-binding EF-hand family protein
Locationchr12: 5486939 .. 5487352 (-)
RNA-Seq ExpressionLag0014878
SyntenyLag0014878
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCGTAGAATTACTGGACGGCGCCACCATTGTCAACTTCGTCGAAGACGAAGAAGCATTCACCGCTTCGATCAGAGAGCGTTTCAGCCACCTCGACGCCGACGGAGATGGCGTCCTCTCCTACGCAGAGATGTTGAAAGAATTACACACTCTCAGAGTCTGCGAAACCGACTTTGGAATCGACGTCAAACCCGACCCGGACGAACTCTCCGACCTCTACGGCGCCGTTTTCAGCCACTTCGATCAGGACTCCGACGGAAAAGTTGATCTGGCTGAGTTCACGACCGGAACCAAGAAGATGATGCTGGCCATTGCCGAAGGAATGGGCTTCTTGCCGATCCAAATGGCGTTGGAGAAAGATGGTTTCTTGATGAAGGCCGTTCAGAGAGAAGCCGCCAAAGCGCTTGCTTGA

mRNA sequence

ATGAGCGTAGAATTACTGGACGGCGCCACCATTGTCAACTTCGTCGAAGACGAAGAAGCATTCACCGCTTCGATCAGAGAGCGTTTCAGCCACCTCGACGCCGACGGAGATGGCGTCCTCTCCTACGCAGAGATGTTGAAAGAATTACACACTCTCAGAGTCTGCGAAACCGACTTTGGAATCGACGTCAAACCCGACCCGGACGAACTCTCCGACCTCTACGGCGCCGTTTTCAGCCACTTCGATCAGGACTCCGACGGAAAAGTTGATCTGGCTGAGTTCACGACCGGAACCAAGAAGATGATGCTGGCCATTGCCGAAGGAATGGGCTTCTTGCCGATCCAAATGGCGTTGGAGAAAGATGGTTTCTTGATGAAGGCCGTTCAGAGAGAAGCCGCCAAAGCGCTTGCTTGA

Coding sequence (CDS)

ATGAGCGTAGAATTACTGGACGGCGCCACCATTGTCAACTTCGTCGAAGACGAAGAAGCATTCACCGCTTCGATCAGAGAGCGTTTCAGCCACCTCGACGCCGACGGAGATGGCGTCCTCTCCTACGCAGAGATGTTGAAAGAATTACACACTCTCAGAGTCTGCGAAACCGACTTTGGAATCGACGTCAAACCCGACCCGGACGAACTCTCCGACCTCTACGGCGCCGTTTTCAGCCACTTCGATCAGGACTCCGACGGAAAAGTTGATCTGGCTGAGTTCACGACCGGAACCAAGAAGATGATGCTGGCCATTGCCGAAGGAATGGGCTTCTTGCCGATCCAAATGGCGTTGGAGAAAGATGGTTTCTTGATGAAGGCCGTTCAGAGAGAAGCCGCCAAAGCGCTTGCTTGA

Protein sequence

MSVELLDGATIVNFVEDEEAFTASIRERFSHLDADGDGVLSYAEMLKELHTLRVCETDFGIDVKPDPDELSDLYGAVFSHFDQDSDGKVDLAEFTTGTKKMMLAIAEGMGFLPIQMALEKDGFLMKAVQREAAKALA
Homology
BLAST of Lag0014878 vs. NCBI nr
Match: XP_038887184.1 (uncharacterized protein LOC120077371 [Benincasa hispida])

HSP 1 Score: 215.3 bits (547), Expect = 3.3e-52
Identity = 109/137 (79.56%), Postives = 116/137 (84.67%), Query Frame = 0

Query: 1   MSVELLDGATIVNFVEDEEAFTASIRERFSHLDADGDGVLSYAEMLKELHTLRVCETDFG 60
           MSVELLDGATIVNFVEDEEAF    RE F+HLD DGDG LSYAEMLKELH+LRV E DFG
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFNHLDTDGDGFLSYAEMLKELHSLRVSEADFG 60

Query: 61  IDVKPDPDELSDLYGAVFSHFDQDSDGKVDLAEFTTGTKKMMLAIAEGMGFLPIQMALEK 120
            DVKPD D+LS LYGAVF  FD+DS+GKVD+ EF  G K MMLAIAEGMGFLPIQMALEK
Sbjct: 61  TDVKPDADDLSRLYGAVFGQFDRDSNGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

Query: 121 DGFLMKAVQREAAKALA 138
           DGFLMKAVQREA KA +
Sbjct: 121 DGFLMKAVQREATKAFS 137

BLAST of Lag0014878 vs. NCBI nr
Match: XP_004144609.1 (uncharacterized protein LOC101208968 [Cucumis sativus] >KAE8645726.1 hypothetical protein Csa_020280 [Cucumis sativus])

HSP 1 Score: 210.3 bits (534), Expect = 1.0e-50
Identity = 108/137 (78.83%), Postives = 112/137 (81.75%), Query Frame = 0

Query: 1   MSVELLDGATIVNFVEDEEAFTASIRERFSHLDADGDGVLSYAEMLKELHTLRVCETDFG 60
           MSVELLDGATIVNFVEDEEAF    RE F+ LD D DG LSYAEMLKELH LRV E DFG
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKELHALRVSEADFG 60

Query: 61  IDVKPDPDELSDLYGAVFSHFDQDSDGKVDLAEFTTGTKKMMLAIAEGMGFLPIQMALEK 120
            DVKPD DELS LY AVF  FD+DSDGKVD+ EF  G K MMLAIAEGMGFLPIQMALEK
Sbjct: 61  TDVKPDADELSQLYSAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

Query: 121 DGFLMKAVQREAAKALA 138
           DGFLMKAVQREA KA +
Sbjct: 121 DGFLMKAVQREATKAFS 137

BLAST of Lag0014878 vs. NCBI nr
Match: XP_008455505.1 (PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo] >KAA0059136.1 uncharacterized protein E6C27_scaffold430G00460 [Cucumis melo var. makuwa] >TYK21611.1 uncharacterized protein E5676_scaffold150G00080 [Cucumis melo var. makuwa])

HSP 1 Score: 206.8 bits (525), Expect = 1.2e-49
Identity = 107/137 (78.10%), Postives = 112/137 (81.75%), Query Frame = 0

Query: 1   MSVELLDGATIVNFVEDEEAFTASIRERFSHLDADGDGVLSYAEMLKELHTLRVCETDFG 60
           MSVELLDGATIVNFVEDEEAF    RE F+ LD D DG LSYAEMLKE +TLRV E DFG
Sbjct: 1   MSVELLDGATIVNFVEDEEAFNGWTREHFTRLDTDRDGFLSYAEMLKEFYTLRVSEADFG 60

Query: 61  IDVKPDPDELSDLYGAVFSHFDQDSDGKVDLAEFTTGTKKMMLAIAEGMGFLPIQMALEK 120
            DVK D DELS LYGAVF  FD+DSDGKVD+ EF  G K MMLAIAEGMGFLPIQMALEK
Sbjct: 61  TDVKLDADELSQLYGAVFKQFDRDSDGKVDVDEFMMGMKNMMLAIAEGMGFLPIQMALEK 120

Query: 121 DGFLMKAVQREAAKALA 138
           DGFLMKAVQREA KA +
Sbjct: 121 DGFLMKAVQREATKAFS 137

BLAST of Lag0014878 vs. NCBI nr
Match: XP_022135904.1 (uncharacterized protein LOC111007741 [Momordica charantia])

HSP 1 Score: 205.7 bits (522), Expect = 2.6e-49
Identity = 101/134 (75.37%), Postives = 118/134 (88.06%), Query Frame = 0

Query: 1   MSVELLDGATIVNFVEDEEAFTASIRERFSHLDADGDGVLSYAEMLKELHTLRVCETDFG 60
           MSVE+LD ATI+NFVEDEEAF A IRERF HLDAD DGVLSYAEML+ELH+LRV ET FG
Sbjct: 1   MSVEILDSATIINFVEDEEAFGAWIRERFRHLDADRDGVLSYAEMLQELHSLRVYETHFG 60

Query: 61  IDVKPDPDELSDLYGAVFSHFDQDSDGKVDLAEFTTGTKKMMLAIAEGMGFLPIQMALEK 120
           +D KPDPDELS LYGAVF  FD++S+G+VDL EF  GTK++M+AIAEGMGFLP+QMALE+
Sbjct: 61  VDAKPDPDELSRLYGAVFMQFDRNSNGEVDLEEFMAGTKRIMVAIAEGMGFLPLQMALEE 120

Query: 121 DGFLMKAVQREAAK 135
           DGFLMKAV+ E ++
Sbjct: 121 DGFLMKAVRSEKSR 134

BLAST of Lag0014878 vs. NCBI nr
Match: XP_038888629.1 (uncharacterized protein LOC120078427 [Benincasa hispida])

HSP 1 Score: 194.1 bits (492), Expect = 7.8e-46
Identity = 97/137 (70.80%), Postives = 111/137 (81.02%), Query Frame = 0

Query: 1   MSVELLDGATIVNFVEDEEAFTASIRERFSHLDADGDGVLSYAEMLKELHTLRVCETDFG 60
           MSVE+LD ATIVNFVEDEEAF+ SIRERFSHLD D DGVL Y EMLKEL +LRV ET FG
Sbjct: 1   MSVEILDSATIVNFVEDEEAFSDSIRERFSHLDLDRDGVLCYGEMLKELQSLRVLETHFG 60

Query: 61  IDVKPDPDELSDLYGAVFSHFDQDSDGKVDLAEFTTGTKKMMLAIAEGMGFLPIQMALEK 120
           ID KPDPDELS +YG++FS FD+D +GKVDL EF   TKKMMLA+A G+GF P+QM LE+
Sbjct: 61  IDTKPDPDELSSVYGSLFSQFDRDCNGKVDLGEFMAETKKMMLAMANGIGFSPVQMLLEE 120

Query: 121 DGFLMKAVQREAAKALA 138
           + FL KAV RE+ K  A
Sbjct: 121 NSFLKKAVDRESTKLAA 137

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038887184.13.3e-5279.56uncharacterized protein LOC120077371 [Benincasa hispida][more]
XP_004144609.11.0e-5078.83uncharacterized protein LOC101208968 [Cucumis sativus] >KAE8645726.1 hypothetica... [more]
XP_008455505.11.2e-4978.10PREDICTED: uncharacterized protein LOC103495656 [Cucumis melo] >KAA0059136.1 unc... [more]
XP_022135904.12.6e-4975.37uncharacterized protein LOC111007741 [Momordica charantia][more]
XP_038888629.17.8e-4670.80uncharacterized protein LOC120078427 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002048EF-hand domainSMARTSM00054efh_1coord: 24..52
e-value: 0.33
score: 20.0
coord: 73..101
e-value: 0.27
score: 20.3
IPR002048EF-hand domainPFAMPF13499EF-hand_7coord: 24..96
e-value: 6.8E-11
score: 42.5
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 20..55
score: 9.93856
IPR002048EF-hand domainPROSITEPS50222EF_HAND_2coord: 69..104
score: 10.914899
NoneNo IPR availableGENE3D1.10.238.10coord: 6..109
e-value: 2.0E-13
score: 52.1
NoneNo IPR availablePANTHERPTHR34574CALCIUM-BINDING EF-HAND FAMILY PROTEIN-RELATEDcoord: 1..134
NoneNo IPR availablePANTHERPTHR34574:SF10CALCIUM-BINDING EF-HAND FAMILY PROTEIN-LIKEcoord: 1..134
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 33..45
IPR018247EF-Hand 1, calcium-binding sitePROSITEPS00018EF_HAND_1coord: 82..94
IPR011992EF-hand domain pairSUPERFAMILY47473EF-handcoord: 23..98

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0014878.1Lag0014878.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005509 calcium ion binding