Lag0012076 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0012076
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	ER membrane protein complex subunit 8/9 homolog
Location	chr1: 37083701 .. 37086991 (+)
RNA-Seq Expression	Lag0012076
Synteny	Lag0012076

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGGCGATCTACGCTTTGAGATCTCTCAGAATGCCTACATAAAGCTCGTTCTCCATGCCCTAAAGCACCGGACCTCCGCCGTCAACGGAGTCCTCCTAGGTCGTAACTCCGGCCCCAATGATGTCGTCGAAATTGCCGATTCCGTCCCTCTCTTCCACTCAAGCATCGGTCTCCTCCCTCAGCTCGAGATATCTCTCCTTCTGGTACTCCTTTTGAACCATTTTGCAGCGAAATTTTTTGTTTAGATTTCTGTGGACTGGTGGCTCATGTGAGAATTTGTTTTGTTTGACACCGTTTTTACTGCCTTTTTACTTGCGTATTGGAGATCTGTGATTGATGAGCATGCTTGATTTGGAATCAGATTGAAGAATATTATGCGGCTAAGGGATTGAGTATCGTGGGATATTTTCATGCTAATGAGCGGTTTGATGACTATGAGGTCGGCGGTGTCGCCAAGAATATTGGTGATCATATCTCTCGATCATTTCCTCACGCTGCTCTTCTTTTGGTAAGATATGTCTTATTGTACTTAAACTGTATTGTTTTGTATATTTCTGTTGTTTGATTTATCCTCCTCTGCAGTTAGATCACAGAAAGCTTGAAGCTTTGCCAAAGAGAAAGGATAGGAGCCCTGTAATGGAGGTGAGTTTTTGGCTATAGAATTCTTTTCATGGTTTGTAAATTGTATTTGTGCGCTTCATTAGAATTTCCACCATACTTTCCTCTTTTAATTCTGTTTCAAAATAATTTAAACGAGCAATTTTATATCGCAAAAATGAAAAATTTAAATATTGTTGGTTGATATGGCGTTCAATGGGACCAACTACTTTGTTCAGTTTTTGAAGGACAGTGGAACATAGGAATATGAAGCAAATGTCAAATTGGGATAGCTTTTTGTAATCTCTCAGGACTTACCATATCTTTTTGTAAATTTCATACATCAATGAAATTGTTTCTTATCCAAAAAAAAGAAAAAAGAAAAAAGGACAGTGAAACATGGTAAAAATAACTTTGTTACATCATTGATGATTCATTCATTTCTTGGGGGGACGTTCTTATAGTTTTCCCAATTACTAGATATGACACAATGCTAAAATGTCACGAGAAGGCTTTATTTTAATTCTATTGGAGTCTTTTGACAATCTCTTGATTATCTTCCATTGGTGGAGACTTGATATCATTAACTACTACTGATGCTATTTGAGATACAAGGAAATGTATGTTGAAGGTTTTCACTGACTTTCTGTCTTGTTTATCTTAAATTCCACCTTGTAACTAGACCTTTAAGTTTATTGAACTTTCTGAGCCCTTTCTAATGTTATTTGAAATTTGGTTTAGCTTGTACTTATTCTACATTGAAACTGATGAAGTATGTCAGGTTTCTTTCAAGGAGGTGAGAGCTTGAGAGAGGCAATAGGAGTGTGATCTCCAATTTTCCTTAATGTTGGAGCTGCTCGATCTTTTAGATGTAGGATTGAACATAAGATGTTCAACATGGAATTTGAGGATGGATATAGGAGAAACATCATTTCATCGTTTTTGCCCAATAAAATGGCGAGTCATTGCACTCCTTTCTTTAGTTTGCCCCTCAACCACCAGAGTTTATGCATAACGTTCTATATCAAAGGAGAATCTTGAGACCTACATTGTAGGAGGAACGTCAGTGATGAGGAGGCAGTGGAATAGATCTTTATCTCTTGTATTTTGGGGGCTTCTTTGGACCCTAAACCAATTATCAGAAAGTGGATGCCTGAAGCTATGGGGATTTTCTGTTGTAAATCCTTTTATGATTTTTTGAGGCAAGATCCAAGTATTACACTGAATAAGATTGACTTGACTTGGAAGTTCGAGATCTCAAAGAAGTTTATTCTTTTGCCTTTATGGGACAGCATTTAGGAGAAGTTGTAGTGGCATTTGATCTTTGTTTTGGGCTACTAGTATTGATTATTATCTCGTTTACGTTCAGGTTGAACCATCAGTTTGCCCTCCCCTATGGTATACGTTTCCTTATTAGTACATCTTTAGCCTCAGAAGTGCAGAAGATCACTTGTTAACTTTTATTTTAAGGTCCGAATTTGTTGGAGGAGGTAATAGTTATATGTTGTCTTACTGGTCAAAAGTATCAATTTTGAGTTGAACTCTGTTAGGCCCCCCGAGTACCATTCCTTATGAAAGGAGGGATGAGAAATTAGTTAGAAGGTCAGGTGCATGGGAGCGAGGAACGACCTGAGGTTTGTTGTGAGAGAATGTCGTGGGGGTGGGGGTGGGGTGGGGGGGTTAGTATGATTATTTAGGTAAGAAGGGAGTTGTATTGTTTTCTTTTATGCTTCTGTCTATGAACAAGTTGTAGAAGAAGAGGAAAGATACCGGCCCTCTCTAATCGGCTGGGAGTTTTTGTATTGCCTTAGGGCATTGTTCCTCCTTTGTTTATTAATATTTCAGAAGGGAGGTACCTTACAAACTCCAGCCCCTGTAGTTCATAAGATCAATCTAGATGTGTTAGTGACAGGCAATTGTTTAACCCAGAAGATAAAAATATCTCGACAAAGTCAAACTCATTACTAATTTCAAAAAAAAAAAAAGTCAAACTCATTACTAAAGTAAATGACAGTAACTGATTGCTGCTTGTTTCAATCGCTTTCCTTTTTTTTTTTTTTTTCTTCTCTTAAGCATCATTATTTGCTCTCTATCTAATGTCAAACTTGTAGTCTAAAATGGCGAGTTGTGTTGTATTAAGCTTCTTTGGCATTTAGATACTATCGAGATAGTATTTCATTTTAAAAATGACTGTAATTTCTGAAGAACGATAATTCATTGTCCTGGCTATGTTTTGCTGCCACTCTTTGCGACCTGACCAGCAGTCGTGAAATCTAGGAACCCTTTTAAACTATAGGCCTTGTCTATGTTGCTACCATGAAATAAAATAGACCCTAAGCATGAGAAGATGATTGCATAATGTGGTTCTCATTCCATTTTTTTTTTCATTATGTTGCCGATCAGCTATACACTAAGGAAACTTCCAAGAATTGGAAGCTTGCTGGATCAGATGGAAGCAGCCAGCTAACGATCAAGGAACCGTCAGCAAATGTTGTCCTATTGGATTTCATATCATCTGAAAAATGGCACGACGTGGTGGATTTTGACGATCACCTCGACGACATTAGCAAGTATGATACACAATGCCCCCTCTTTTTGAATATTGCATCAATCATGGATATAGTTAAACCTTATTCTTTTAAACTTTGAAAACTTTCAGGGATTGGTTGAACCCAGACCTCTTCAAGTAG

mRNA sequence

ATGGGCGGCGATCTACGCTTTGAGATCTCTCAGAATGCCTACATAAAGCTCGTTCTCCATGCCCTAAAGCACCGGACCTCCGCCGTCAACGGAGTCCTCCTAGGTCGTAACTCCGGCCCCAATGATGTCGTCGAAATTGCCGATTCCGTCCCTCTCTTCCACTCAAGCATCGGTCTCCTCCCTCAGCTCGAGATATCTCTCCTTCTGATTGAAGAATATTATGCGGCTAAGGGATTGAGTATCGTGGGATATTTTCATGCTAATGAGCGGTTTGATGACTATGAGGTCGGCGGTGTCGCCAAGAATATTGGTGATCATATCTCTCGATCATTTCCTCACGCTGCTCTTCTTTTGTTAGATCACAGAAAGCTTGAAGCTTTGCCAAAGAGAAAGGATAGGAGCCCTGTAATGGAGCTATACACTAAGGAAACTTCCAAGAATTGGAAGCTTGCTGGATCAGATGGAAGCAGCCAGCTAACGATCAAGGAACCGTCAGCAAATGTTGTCCTATTGGATTTCATATCATCTGAAAAATGGCACGACGTGGTGGATTTTGACGATCACCTCGACGACATTAGCAAGGATTGGTTGAACCCAGACCTCTTCAAGTAG

Coding sequence (CDS)

ATGGGCGGCGATCTACGCTTTGAGATCTCTCAGAATGCCTACATAAAGCTCGTTCTCCATGCCCTAAAGCACCGGACCTCCGCCGTCAACGGAGTCCTCCTAGGTCGTAACTCCGGCCCCAATGATGTCGTCGAAATTGCCGATTCCGTCCCTCTCTTCCACTCAAGCATCGGTCTCCTCCCTCAGCTCGAGATATCTCTCCTTCTGATTGAAGAATATTATGCGGCTAAGGGATTGAGTATCGTGGGATATTTTCATGCTAATGAGCGGTTTGATGACTATGAGGTCGGCGGTGTCGCCAAGAATATTGGTGATCATATCTCTCGATCATTTCCTCACGCTGCTCTTCTTTTGTTAGATCACAGAAAGCTTGAAGCTTTGCCAAAGAGAAAGGATAGGAGCCCTGTAATGGAGCTATACACTAAGGAAACTTCCAAGAATTGGAAGCTTGCTGGATCAGATGGAAGCAGCCAGCTAACGATCAAGGAACCGTCAGCAAATGTTGTCCTATTGGATTTCATATCATCTGAAAAATGGCACGACGTGGTGGATTTTGACGATCACCTCGACGACATTAGCAAGGATTGGTTGAACCCAGACCTCTTCAAGTAG

Protein sequence

MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLLPQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDHRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWHDVVDFDDHLDDISKDWLNPDLFK

Homology

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BLAST of Lag0012076 vs. NCBI nr
Match: XP_008455761.1 (PREDICTED: ER membrane protein complex subunit 8/9 homolog [Cucumis melo] >KAA0025782.1 ER membrane protein complex subunit 8/9-like protein [Cucumis melo var. makuwa] >TYK09673.1 ER membrane protein complex subunit 8/9-like protein [Cucumis melo var. makuwa])

HSP 1 Score: 392.5 bits (1007), Expect = 2.2e-105
Identity = 195/203 (96.06%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHR+SAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRSSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQL IKEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

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BLAST of Lag0012076 vs. NCBI nr
Match: XP_038880935.1 (ER membrane protein complex subunit 8/9 homolog [Benincasa hispida])

HSP 1 Score: 392.5 bits (1007), Expect = 2.2e-105
Identity = 195/203 (96.06%), Postives = 198/203 (97.54%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MG DLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGSDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLE LPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEVLPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

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BLAST of Lag0012076 vs. NCBI nr
Match: XP_004144311.1 (ER membrane protein complex subunit 8/9 homolog [Cucumis sativus] >KGN54668.1 hypothetical protein Csa_012212 [Cucumis sativus])

HSP 1 Score: 391.7 bits (1005), Expect = 3.8e-105
Identity = 194/203 (95.57%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRT+AVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTTAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQL +KEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

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BLAST of Lag0012076 vs. NCBI nr
Match: KAG6595444.1 (ER membrane protein complex subunit 8/9-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7027444.1 ER membrane protein complex subunit 8/9-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 387.5 bits (994), Expect = 7.1e-104
Identity = 192/203 (94.58%), Postives = 198/203 (97.54%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SG NDV+EIA+SVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGSNDVIEIAESVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDY+ GGVAKNI DHISRSFPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYDAGGVAKNIADHISRSFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPV+ELYT+ETSKNWKLAGSDGSSQLT+KEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVVELYTRETSKNWKLAGSDGSSQLTMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

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BLAST of Lag0012076 vs. NCBI nr
Match: XP_023518172.1 (ER membrane protein complex subunit 8/9 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 386.3 bits (991), Expect = 1.6e-103
Identity = 192/203 (94.58%), Postives = 198/203 (97.54%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SG NDV+EIA+SVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGSNDVIEIAESVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDY+VGGVAKNI DHISRSFPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYDVGGVAKNIADHISRSFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPV+ELYT+ETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVVELYTRETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
            V+DFDDHLDDISKDWLNPDLFK
Sbjct: 181 GVMDFDDHLDDISKDWLNPDLFK 203

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BLAST of Lag0012076 vs. ExPASy Swiss-Prot
Match: Q9FG71 (ER membrane protein complex subunit 8/9 homolog OS=Arabidopsis thaliana OX=3702 GN=EMB2731 PE=2 SV=1)

HSP 1 Score: 296.2 bits (757), Expect = 2.8e-79
Identity = 144/201 (71.64%), Postives = 176/201 (87.56%), Query Frame = 0


Query: 3   GDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPND-VVEIADSVPLFHSSIGLLP 62
           G+L++EISQNAYIKLVLH+L+H+T+AVNGVL+GR S  +D VVEI+DSVPLFHS++ LLP
Sbjct: 7   GELKYEISQNAYIKLVLHSLRHKTAAVNGVLVGRISPKDDGVVEISDSVPLFHSNLALLP 66

Query: 63  QLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDH 122
            LEISL++IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIGDHISR FP A +LLL++
Sbjct: 67  PLEISLIMIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIGDHISRYFPQAPILLLNN 126

Query: 123 RKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWHD 182
           +KLEAL K K+RSPVM+L  K+ SKNW++ G+DG S+L +KEPSANVVL D+ISSEKW D
Sbjct: 127 KKLEALSKGKERSPVMQLCVKDASKNWRVVGADGGSKLLLKEPSANVVLSDYISSEKWKD 186

Query: 183 VVDFDDHLDDISKDWLNPDLF 203
           V D DDHLDD++KDWLNP LF
Sbjct: 187 VTDVDDHLDDVTKDWLNPGLF 207

[Close]

BLAST of Lag0012076 vs. ExPASy Swiss-Prot
Match: Q9DB76 (ER membrane protein complex subunit 9 OS=Mus musculus OX=10090 GN=Emc9 PE=1 SV=1)

HSP 1 Score: 109.4 bits (272), Expect = 4.9e-23
Identity = 67/194 (34.54%), Postives = 103/194 (53.09%), Query Frame = 0


Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLLPQLEISL 67
           EIS  AY K+ LHA ++  +AVNG+LL   +G  + + + D VPLFHS + L   LE++L
Sbjct: 5   EISARAYGKMCLHASRYPHAAVNGLLLAPATGSGECLCLTDCVPLFHSHLALSVMLEVAL 64

Query: 68  LLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDHRKLEAL 127
             ++ + A  GL + GY+HAN   DD   G +A  I   I+  FP A L++LD++KL   
Sbjct: 65  NQVDVWGAQAGLVVAGYYHANAVLDDQSPGPLALKIAGRIAEFFPRAVLIMLDNKKLVTR 124

Query: 128 PKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWHDVVDFDD 187
           P+     PV+ L     ++  +    D +  +      +  ++   +       +VDFD 
Sbjct: 125 PR---VPPVIVL----ENQGLQWVPKDKNLVMWRDWEESRQMVGALLEGRAHQHLVDFDC 184

Query: 188 HLDDISKDWLNPDL 202
           HLDDI +DW N  L
Sbjct: 185 HLDDIRQDWTNQRL 191

[Close]

BLAST of Lag0012076 vs. ExPASy Swiss-Prot
Match: Q5FVL2 (ER membrane protein complex subunit 8 OS=Rattus norvegicus OX=10116 GN=Emc8 PE=2 SV=1)

HSP 1 Score: 108.6 bits (270), Expect = 8.3e-23
Identity = 63/204 (30.88%), Postives = 100/204 (49.02%), Query Frame = 0


Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNS--------GPNDVVEIADSVPLFHSSIGL 67
           +++  AY K+VLH  K+   AVNG+L+            G  +     D +PLFH ++ L
Sbjct: 5   KLTTQAYCKMVLHGAKYPHCAVNGLLVAERQRPRKEHPPGAGNHTLFVDCIPLFHGTLAL 64

Query: 68  LPQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLL 127
            P LE++L LI+ +       I GY+ ANER  D     VA+ +   I+  F  AAL+++
Sbjct: 65  TPMLEVALTLIDSWCKDNSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFSDAALIMV 124

Query: 128 DHRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKW 187
           D+ K          +P + +Y +  ++ W+    D         P A  +    + S  +
Sbjct: 125 DNAKF----TMDCAAPTIHVYEQHENR-WRC--RDPHHDYCEDWPEAQRISASLLDSRSY 184

Query: 188 HDVVDFDDHLDDISKDWLNPDLFK 204
             +VDFD+HLDDI  DW NP++ K
Sbjct: 185 ETLVDFDNHLDDIRSDWTNPEINK 201

[Close]

BLAST of Lag0012076 vs. ExPASy Swiss-Prot
Match: O70378 (ER membrane protein complex subunit 8 OS=Mus musculus OX=10090 GN=Emc8 PE=1 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.4e-22
Identity = 63/204 (30.88%), Postives = 99/204 (48.53%), Query Frame = 0


Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNS--------GPNDVVEIADSVPLFHSSIGL 67
           +++  AY K+VLH  K+   AVNG+L+            G        D +PLFH ++ L
Sbjct: 5   KLTTQAYCKMVLHGAKYPHCAVNGLLVAERQRPRKEHPPGAGSHTLFVDCIPLFHGTLAL 64

Query: 68  LPQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLL 127
            P LE++L LI+ +       I GY+ ANER  D     VA+ +   I+  F  AAL+++
Sbjct: 65  TPMLEVALTLIDSWCKDNSYVIAGYYQANERVKDASPNQVAEKVASRIAEGFGDAALIMV 124

Query: 128 DHRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKW 187
           D+ K          +P + +Y +  ++ W+    D         P A  +    + S  +
Sbjct: 125 DNAKF----TMDCAAPTIHVYEQHENR-WRC--RDPHHDYCEDWPEAQRISASLLDSRSY 184

Query: 188 HDVVDFDDHLDDISKDWLNPDLFK 204
             +VDFD+HLDDI  DW NP++ K
Sbjct: 185 ETLVDFDNHLDDIRSDWTNPEINK 201

[Close]

BLAST of Lag0012076 vs. ExPASy Swiss-Prot
Match: Q5U1W7 (ER membrane protein complex subunit 9 OS=Rattus norvegicus OX=10116 GN=Emc9 PE=2 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 7.1e-22
Identity = 65/194 (33.51%), Postives = 102/194 (52.58%), Query Frame = 0


Query: 8   EISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLLPQLEISL 67
           EIS  AY K+ LHA ++  +AVNG+LL   +   + + + D VPLFHS + L   LE++L
Sbjct: 5   EISARAYGKMCLHASRYPHAAVNGLLLAPATRSGECLCLTDCVPLFHSHLALSVMLEVAL 64

Query: 68  LLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDHRKLEAL 127
             ++ +    GL + GY+HAN   DD   G +A  I   I+  FP+A L++LD++KL   
Sbjct: 65  NQVDVWATQAGLVVAGYYHANAVLDDQSPGPLALKIAGRIAEFFPNAVLIMLDNKKLVTW 124

Query: 128 PKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWHDVVDFDD 187
           P+     PV+ L     ++  +    D +  +      +  ++   +       +VDFD 
Sbjct: 125 PR---VPPVIVL----ENQGLQWVPKDKNLVMWRDWEESRQMVGALLEGRAHQHLVDFDC 184

Query: 188 HLDDISKDWLNPDL 202
           HLDDI +DW N  L
Sbjct: 185 HLDDIRQDWTNQRL 191

[Close]

BLAST of Lag0012076 vs. ExPASy TrEMBL
Match: A0A5A7SKW9 (ER membrane protein complex subunit 8/9-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00760 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-105
Identity = 195/203 (96.06%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHR+SAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRSSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQL IKEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

[Close]

BLAST of Lag0012076 vs. ExPASy TrEMBL
Match: A0A1S3C2C1 (ER membrane protein complex subunit 8/9 homolog OS=Cucumis melo OX=3656 GN=LOC103495860 PE=3 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.1e-105
Identity = 195/203 (96.06%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHR+SAVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRSSAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQL IKEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMIKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

[Close]

BLAST of Lag0012076 vs. ExPASy TrEMBL
Match: A0A0A0KYR9 (MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419600 PE=3 SV=1)

HSP 1 Score: 391.7 bits (1005), Expect = 1.8e-105
Identity = 194/203 (95.57%), Postives = 199/203 (98.03%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRT+AVNGVLLGR SGPNDV+EI+DSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTTAVNGVLLGRISGPNDVIEISDSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQL +KEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLMMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

[Close]

BLAST of Lag0012076 vs. ExPASy TrEMBL
Match: A0A6J1HRP6 (ER membrane protein complex subunit 8/9 homolog OS=Cucurbita maxima OX=3661 GN=LOC111466107 PE=3 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 7.7e-104
Identity = 192/203 (94.58%), Postives = 198/203 (97.54%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGR SG NDV+EIA+SVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRISGSNDVIEIAESVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGLSIVGYFHAN RFDDY+VGGVAKNI DHISRSFPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANVRFDDYDVGGVAKNIADHISRSFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPV+ELYT+ETSKNWKLAGSDGSSQLT+KEPSANVVLLDFISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVVELYTRETSKNWKLAGSDGSSQLTMKEPSANVVLLDFISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

[Close]

BLAST of Lag0012076 vs. ExPASy TrEMBL
Match: A0A6J1GG75 (ER membrane protein complex subunit 8/9 homolog OS=Cucurbita moschata OX=3662 GN=LOC111453869 PE=3 SV=1)

HSP 1 Score: 386.0 bits (990), Expect = 1.0e-103
Identity = 190/203 (93.60%), Postives = 197/203 (97.04%), Query Frame = 0


Query: 1   MGGDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPNDVVEIADSVPLFHSSIGLL 60
           MGGDLRFEISQNAYIKL+LHALKHRTSAVNG+LLGR SGP DV+EIADSVPLFHSSIGLL
Sbjct: 1   MGGDLRFEISQNAYIKLILHALKHRTSAVNGLLLGRISGPTDVIEIADSVPLFHSSIGLL 60

Query: 61  PQLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLD 120
           PQLEISLLLIEEYYAAKGL+IVGYFHANERFDDYEVGGVAKNIGDHISR FPHAALLLLD
Sbjct: 61  PQLEISLLLIEEYYAAKGLNIVGYFHANERFDDYEVGGVAKNIGDHISRFFPHAALLLLD 120

Query: 121 HRKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWH 180
           HRKLEALPKRKDRSPVMELYT+ET KNWKLAGSDGSSQLTIKEP AN+VLLD+ISSEKW 
Sbjct: 121 HRKLEALPKRKDRSPVMELYTRETPKNWKLAGSDGSSQLTIKEPLANMVLLDYISSEKWQ 180

Query: 181 DVVDFDDHLDDISKDWLNPDLFK 204
           DVVDFDDHLDDISKDWLNPDLFK
Sbjct: 181 DVVDFDDHLDDISKDWLNPDLFK 203

[Close]

BLAST of Lag0012076 vs. TAIR 10
Match: AT5G55940.1 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 296.2 bits (757), Expect = 2.0e-80
Identity = 144/201 (71.64%), Postives = 176/201 (87.56%), Query Frame = 0


Query: 3   GDLRFEISQNAYIKLVLHALKHRTSAVNGVLLGRNSGPND-VVEIADSVPLFHSSIGLLP 62
           G+L++EISQNAYIKLVLH+L+H+T+AVNGVL+GR S  +D VVEI+DSVPLFHS++ LLP
Sbjct: 7   GELKYEISQNAYIKLVLHSLRHKTAAVNGVLVGRISPKDDGVVEISDSVPLFHSNLALLP 66

Query: 63  QLEISLLLIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLLLDH 122
            LEISL++IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIGDHISR FP A +LLL++
Sbjct: 67  PLEISLIMIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIGDHISRYFPQAPILLLNN 126

Query: 123 RKLEALPKRKDRSPVMELYTKETSKNWKLAGSDGSSQLTIKEPSANVVLLDFISSEKWHD 182
           +KLEAL K K+RSPVM+L  K+ SKNW++ G+DG S+L +KEPSANVVL D+ISSEKW D
Sbjct: 127 KKLEALSKGKERSPVMQLCVKDASKNWRVVGADGGSKLLLKEPSANVVLSDYISSEKWKD 186

Query: 183 VVDFDDHLDDISKDWLNPDLF 203
           V D DDHLDD++KDWLNP LF
Sbjct: 187 VTDVDDHLDDVTKDWLNPGLF 207

[Close]

BLAST of Lag0012076 vs. TAIR 10
Match: AT5G51620.3 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 84.0 bits (206), Expect = 1.6e-16
Identity = 39/50 (78.00%), Postives = 44/50 (88.00%), Query Frame = 0


Query: 69  LIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIGDHISRSFPHAALLL 119
           +IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIGDHISR FP A +LL
Sbjct: 1   MIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIGDHISRYFPQAPILL 50

[Close]

BLAST of Lag0012076 vs. TAIR 10
Match: AT5G51620.2 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 29/36 (80.56%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 69  LIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIG 105
           +IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIG
Sbjct: 1   MIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIG 36

[Close]

BLAST of Lag0012076 vs. TAIR 10
Match: AT5G51620.1 (Uncharacterised protein family (UPF0172) )

HSP 1 Score: 63.9 bits (154), Expect = 1.7e-10
Identity = 29/36 (80.56%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 69  LIEEYYAAKGLSIVGYFHANERFDDYEVGGVAKNIG 105
           +IEE+Y A+GLSIVGYFHANERFDD E+ GVAKNIG
Sbjct: 1   MIEEHYVAQGLSIVGYFHANERFDDVELCGVAKNIG 36

The following BLAST results are available for this feature:

BLAST of Lag0012076 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_008455761.1	2.2e-105	96.06	PREDICTED: ER membrane protein complex subunit 8/9 homolog [Cucumis melo] >KAA00...	[more]
XP_038880935.1	2.2e-105	96.06	ER membrane protein complex subunit 8/9 homolog [Benincasa hispida]	[more]
XP_004144311.1	3.8e-105	95.57	ER membrane protein complex subunit 8/9 homolog [Cucumis sativus] >KGN54668.1 hy...	[more]
KAG6595444.1	7.1e-104	94.58	ER membrane protein complex subunit 8/9-like protein, partial [Cucurbita argyros...	[more]
XP_023518172.1	1.6e-103	94.58	ER membrane protein complex subunit 8/9 homolog [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0012076 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FG71	2.8e-79	71.64	ER membrane protein complex subunit 8/9 homolog OS=Arabidopsis thaliana OX=3702 ...	[more]
Q9DB76	4.9e-23	34.54	ER membrane protein complex subunit 9 OS=Mus musculus OX=10090 GN=Emc9 PE=1 SV=1	[more]
Q5FVL2	8.3e-23	30.88	ER membrane protein complex subunit 8 OS=Rattus norvegicus OX=10116 GN=Emc8 PE=2...	[more]
O70378	1.4e-22	30.88	ER membrane protein complex subunit 8 OS=Mus musculus OX=10090 GN=Emc8 PE=1 SV=1	[more]
Q5U1W7	7.1e-22	33.51	ER membrane protein complex subunit 9 OS=Rattus norvegicus OX=10116 GN=Emc9 PE=2...	[more]

BLAST of Lag0012076 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7SKW9	1.1e-105	96.06	ER membrane protein complex subunit 8/9-like protein OS=Cucumis melo var. makuwa...	[more]
A0A1S3C2C1	1.1e-105	96.06	ER membrane protein complex subunit 8/9 homolog OS=Cucumis melo OX=3656 GN=LOC10...	[more]
A0A0A0KYR9	1.8e-105	95.57	MPN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G419600 PE=3 SV...	[more]
A0A6J1HRP6	7.7e-104	94.58	ER membrane protein complex subunit 8/9 homolog OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1GG75	1.0e-103	93.60	ER membrane protein complex subunit 8/9 homolog OS=Cucurbita moschata OX=3662 GN...	[more]

BLAST of Lag0012076 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G55940.1	2.0e-80	71.64	Uncharacterised protein family (UPF0172)	[more]
AT5G51620.3	1.6e-16	78.00	Uncharacterised protein family (UPF0172)	[more]
AT5G51620.2	1.7e-10	80.56	Uncharacterised protein family (UPF0172)	[more]
AT5G51620.1	1.7e-10	80.56	Uncharacterised protein family (UPF0172)	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR005366	ER membrane protein complex subunit 8/9	PFAM	PF03665	UPF0172	coord: 7..201 e-value: 1.9E-59 score: 201.0
IPR005366	ER membrane protein complex subunit 8/9	PANTHER	PTHR12941	ER MEMBRANE PROTEIN COMPLEX	coord: 1..203
IPR005366	ER membrane protein complex subunit 8/9	CDD	cd08060	MPN_UPF0172	coord: 9..198 e-value: 7.50113E-72 score: 214.081
None	No IPR available	PANTHER	PTHR12941:SF14	ER MEMBRANE PROTEIN COMPLEX SUBUNIT 8/9 HOMOLOG	coord: 1..203
IPR037518	MPN domain	PROSITE	PS50249	MPN	coord: 7..146 score: 18.256346

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0012076.1	Lag0012076.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0072546	EMC complex