Lag0011681 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0011681
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionpolygalacturonase inhibitor-like
Locationchr1: 30731351 .. 30732334 (+)
RNA-Seq ExpressionLag0011681
SyntenyLag0011681
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCATCCCTCCACTTTGTTTCTCGTCCTCTTCCTCTCCTTCACCATTTCATTTGCAGAAAGATGCAACCCAAATGACAAAAAAGCCCTCCTCGAGATCAAAAAAGCCTTCAACGATCCCTACGTCCTGATCTCATGGAAGCCCGAAGAAGACTGCTGCACTTGGAACTCCGTCGAATGCGGTGAGAAATCCGACCGGATCATCACCATCGACATCGGCCCCGACGACGAACTCTCCGGCCCAATCCCGCCGGCCGTCGGCGACCTACCATTCCTCCAAACCCTGATGCTCCGCAAGCTCCCAAATCTCGTCGGCCCCCTCCCACCCGCCATTGCCAAGCTCCACAACCTCAAATTCCTCGACGTCAGCTGGACCAATATCTCCGGATCCGTCCCCGATTTCCTCGGCACTCTCCCCAACCTCGAATACATCCGCCTCAACTTCAACAATCTCACCGGAACTCTCCCGGCGTCCCTCTCCAAACTCCCAAAGCTCGGCGCTCTGCAATTGGACCGAAACAAGTTGACAGGCCCCATCCCTGACTCCTTCGGCTACTTGAAGGCCGAATCTATCGTCCTGGATCTTTCCCACAACAAACTCTCCGGCAAAATCCCGCCTTCCTTGACGAGATTGGACCTCTTCTCCATCGACTTGTCCAGGAACATGCTGGAGGGCGATGCCTCCATGATATTCGGAGCCCACAAGAAGGCGTGGAAAGTCGTTCTGTCGAGGAATTTGTTGTCGTTTGATATGTCCAAGGTTGTGTTCTCCACCAGCCTGTTTTATTTGGATCTCAACCATAATAAGATATTTGGGGGTTTGCCGCCGCAGATGACCAAACTCAGTAATCTGAATTCTTTCAATGTCAGTTATAATCGGCTGTGCGGACCGATCCCGCAGGGTGGCGAGTTGCAGAGGTTTGATGTTTATTCCTATTTTCATAATAAGTGTTTGTGCGGCGCTCCACTTCCCCGCTGCAAGTGA

mRNA sequence

ATGCATCCCTCCACTTTGTTTCTCGTCCTCTTCCTCTCCTTCACCATTTCATTTGCAGAAAGATGCAACCCAAATGACAAAAAAGCCCTCCTCGAGATCAAAAAAGCCTTCAACGATCCCTACGTCCTGATCTCATGGAAGCCCGAAGAAGACTGCTGCACTTGGAACTCCGTCGAATGCGGTGAGAAATCCGACCGGATCATCACCATCGACATCGGCCCCGACGACGAACTCTCCGGCCCAATCCCGCCGGCCGTCGGCGACCTACCATTCCTCCAAACCCTGATGCTCCGCAAGCTCCCAAATCTCGTCGGCCCCCTCCCACCCGCCATTGCCAAGCTCCACAACCTCAAATTCCTCGACGTCAGCTGGACCAATATCTCCGGATCCGTCCCCGATTTCCTCGGCACTCTCCCCAACCTCGAATACATCCGCCTCAACTTCAACAATCTCACCGGAACTCTCCCGGCGTCCCTCTCCAAACTCCCAAAGCTCGGCGCTCTGCAATTGGACCGAAACAAGTTGACAGGCCCCATCCCTGACTCCTTCGGCTACTTGAAGGCCGAATCTATCGTCCTGGATCTTTCCCACAACAAACTCTCCGGCAAAATCCCGCCTTCCTTGACGAGATTGGACCTCTTCTCCATCGACTTGTCCAGGAACATGCTGGAGGGCGATGCCTCCATGATATTCGGAGCCCACAAGAAGGCGTGGAAAGTCGTTCTGTCGAGGAATTTGTTGTCGTTTGATATGTCCAAGGTTGTGTTCTCCACCAGCCTGTTTTATTTGGATCTCAACCATAATAAGATATTTGGGGGTTTGCCGCCGCAGATGACCAAACTCAGTAATCTGAATTCTTTCAATGTCAGTTATAATCGGCTGTGCGGACCGATCCCGCAGGGTGGCGAGTTGCAGAGGTTTGATGTTTATTCCTATTTTCATAATAAGTGTTTGTGCGGCGCTCCACTTCCCCGCTGCAAGTGA

Coding sequence (CDS)

ATGCATCCCTCCACTTTGTTTCTCGTCCTCTTCCTCTCCTTCACCATTTCATTTGCAGAAAGATGCAACCCAAATGACAAAAAAGCCCTCCTCGAGATCAAAAAAGCCTTCAACGATCCCTACGTCCTGATCTCATGGAAGCCCGAAGAAGACTGCTGCACTTGGAACTCCGTCGAATGCGGTGAGAAATCCGACCGGATCATCACCATCGACATCGGCCCCGACGACGAACTCTCCGGCCCAATCCCGCCGGCCGTCGGCGACCTACCATTCCTCCAAACCCTGATGCTCCGCAAGCTCCCAAATCTCGTCGGCCCCCTCCCACCCGCCATTGCCAAGCTCCACAACCTCAAATTCCTCGACGTCAGCTGGACCAATATCTCCGGATCCGTCCCCGATTTCCTCGGCACTCTCCCCAACCTCGAATACATCCGCCTCAACTTCAACAATCTCACCGGAACTCTCCCGGCGTCCCTCTCCAAACTCCCAAAGCTCGGCGCTCTGCAATTGGACCGAAACAAGTTGACAGGCCCCATCCCTGACTCCTTCGGCTACTTGAAGGCCGAATCTATCGTCCTGGATCTTTCCCACAACAAACTCTCCGGCAAAATCCCGCCTTCCTTGACGAGATTGGACCTCTTCTCCATCGACTTGTCCAGGAACATGCTGGAGGGCGATGCCTCCATGATATTCGGAGCCCACAAGAAGGCGTGGAAAGTCGTTCTGTCGAGGAATTTGTTGTCGTTTGATATGTCCAAGGTTGTGTTCTCCACCAGCCTGTTTTATTTGGATCTCAACCATAATAAGATATTTGGGGGTTTGCCGCCGCAGATGACCAAACTCAGTAATCTGAATTCTTTCAATGTCAGTTATAATCGGCTGTGCGGACCGATCCCGCAGGGTGGCGAGTTGCAGAGGTTTGATGTTTATTCCTATTTTCATAATAAGTGTTTGTGCGGCGCTCCACTTCCCCGCTGCAAGTGA

Protein sequence

MHPSTLFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRFDVYSYFHNKCLCGAPLPRCK
Homology
BLAST of Lag0011681 vs. NCBI nr
Match: XP_022966387.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 518.5 bits (1334), Expect = 4.3e-143
Identity = 248/320 (77.50%), Postives = 272/320 (85.00%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L  F+ FTISFAE CNPNDKKALL IKKAFN+ Y   SWKPEEDCCTW SVEC E S RI
Sbjct: 13  LFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++DI  D+E+SG IPP VGDLPFLQ+LML KLPNLVGP+ PAIAKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRN LEGDASMIFGAH+  WKV LSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
            FDMSKVVFS SLF LDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQRF
Sbjct: 253 EFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
           DVYSYFHNKCLCGAPL  CK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

BLAST of Lag0011681 vs. NCBI nr
Match: XP_022925061.1 (polygalacturonase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 518.5 bits (1334), Expect = 4.3e-143
Identity = 247/320 (77.19%), Postives = 272/320 (85.00%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L  FL FTISFAE CNPNDKKALL IKKAFN+ Y   SWKPEEDCCTW SVEC E S RI
Sbjct: 13  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++DI  D+E+SG IPP VGDLPFLQ+LML KLPNLVGP+ PA+AKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQLDRSKLTGQIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRN LEGDASMIFG H+  WKV LSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGVHRHTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQRF
Sbjct: 253 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
           DVYSYFHNKCLCGAPL  CK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

BLAST of Lag0011681 vs. NCBI nr
Match: KAG6595655.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 518.1 bits (1333), Expect = 5.6e-143
Identity = 247/320 (77.19%), Postives = 272/320 (85.00%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L  FL FTISFAE CNPNDKKALL IKKAFN+ Y   SWKPEEDCCTW SV C E S RI
Sbjct: 13  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVHCDENSHRI 72

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++DI  D+E+SG IPP VGDLPFLQ+LML KLPNLVGP+ PA+AKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGQIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRN LEGDASMIFGAH+  WKV LSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRHTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQRF
Sbjct: 253 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
           DVYSYFHNKCLCGAPL  CK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

BLAST of Lag0011681 vs. NCBI nr
Match: XP_023517556.1 (polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 514.2 bits (1323), Expect = 8.1e-142
Identity = 244/320 (76.25%), Postives = 270/320 (84.38%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L  FL FTISFAE CNPNDKKALL IKKAFN+ Y   SWKPEEDCCTW SV+C E S RI
Sbjct: 17  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVDCDENSHRI 76

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++DI  D+E+SG IPP +GDLPFLQ+LML KLPNLVGP+ P IAKLHNLK LD+SWTNI
Sbjct: 77  ISLDISTDNEISGQIPPYIGDLPFLQSLMLHKLPNLVGPIQPTIAKLHNLKHLDISWTNI 136

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 137 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPSLEFLQLDRSKLTGQIPYSFGDFK 196

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRN LEGDASMIFG H+  WKV LSRNLL
Sbjct: 197 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGEHRHTWKVDLSRNLL 256

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKL NLNSFNVSYNRLCG IP+GGELQRF
Sbjct: 257 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLGNLNSFNVSYNRLCGQIPKGGELQRF 316

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
           DVYSYFHNKCLCGAPL  CK
Sbjct: 317 DVYSYFHNKCLCGAPLESCK 336

BLAST of Lag0011681 vs. NCBI nr
Match: KAG6595656.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 481.1 bits (1237), Expect = 7.6e-132
Identity = 230/320 (71.88%), Postives = 266/320 (83.12%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L++F+ FTISFAE CNPNDKKALL IK+AFN+ Y   SWKPEEDCCTW SV+C E S RI
Sbjct: 17  LLIFIFFTISFAELCNPNDKKALLNIKEAFNNAYYFASWKPEEDCCTWYSVQCDEISHRI 76

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++ I  D ++SG IPP VGDLP+LQ L   KLPNLVGP+ PAIAKLHNLK LD+SWT I
Sbjct: 77  ISLTISVDKQISGQIPPHVGDLPYLQYLTFHKLPNLVGPIQPAIAKLHNLKHLDISWTKI 136

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L NL YI L+F+NL+G +P+SLSKLP L +LQLDR+KLTG IPDSFG  K
Sbjct: 137 SGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSSLQLDRSKLTGQIPDSFGDFK 196

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           AE+  L LSHNKL+GKIPPSL+++D  +IDLSRN LEGDA MIFGAHKKAW V LSRNLL
Sbjct: 197 AENFNLYLSHNKLTGKIPPSLSKVDFSAIDLSRNKLEGDAYMIFGAHKKAWNVDLSRNLL 256

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
           +FDMSKVVFST+LF LD+NHNKIFGGLPPQM+KLS L S NVSYNRLCG IP+G +L+RF
Sbjct: 257 AFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVSYNRLCGQIPKGKKLKRF 316

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
            V SYFHNKCLCG PL  CK
Sbjct: 317 GVDSYFHNKCLCGKPLESCK 335

BLAST of Lag0011681 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 1.8e-107
Identity = 196/325 (60.31%), Postives = 234/325 (72.00%), Query Frame = 0

Query: 11  FLSFTISF--------AERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGE 70
           FLS T+ F        ++ CNP+DKK LL+IKKAF DPYVL SWK + DCC W  V C  
Sbjct: 8   FLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTCDS 67

Query: 71  KSDRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDV 130
            ++RI ++ I    ++SG IP  VGDLP+L+TL   K PNL GP+ PAIAKL  LK L +
Sbjct: 68  TTNRINSLTIFA-GQVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSLRL 127

Query: 131 SWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDS 190
           SWTN+SGSVPDFL  L NL ++ L+FNNLTG +P+SLS+LP LGAL+LDRNKLTG IP S
Sbjct: 128 SWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIPIS 187

Query: 191 FGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVL 250
           FG        L LSHN+LSG IP S  ++D  SIDLSRN LEGDAS+IFG +K    V L
Sbjct: 188 FGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIVDL 247

Query: 251 SRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGG 310
           SRNLL F++SKV F TSL  LD+NHNKI+G +P + T+L N    NVSYNRLCG IP GG
Sbjct: 248 SRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQL-NFQFLNVSYNRLCGQIPVGG 307

Query: 311 ELQRFDVYSYFHNKCLCGAPLPRCK 328
           +LQ FD YSYFHN+CLCGAPLP CK
Sbjct: 308 KLQSFDEYSYFHNRCLCGAPLPSCK 330

BLAST of Lag0011681 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 373.2 bits (957), Expect = 2.9e-102
Identity = 192/325 (59.08%), Postives = 225/325 (69.23%), Query Frame = 0

Query: 4   STLFLVLFLSFTI-SFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGE 63
           S+L LVL  +    S +ERCNP DKK LL+IKKA ++PY+L SW P  DCC W  VEC  
Sbjct: 11  SSLLLVLLATRPCPSLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECDL 70

Query: 64  KSDRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDV 123
            + RI ++ I    +LSG IP AVGDLPFL+TL+ RKL NL G +PPAIAKL +LK + +
Sbjct: 71  TTHRINSLTIF-SGQLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVRL 130

Query: 124 SWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDS 183
           SWTN+SG VP F   L NL Y+ L+FNNL+G +P SLS LP LGAL LDRN LTGPIPDS
Sbjct: 131 SWTNLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPDS 190

Query: 184 FGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVL 243
           FG     +  L LSHN+LSGKIP S    D   +DLSRN LEGD S+ F A+K    V  
Sbjct: 191 FGKFAGSTPGLHLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVDF 250

Query: 244 SRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGG 303
           SRNL  FD+S+V F  SL  LDL+HNKI G LP  MT L +L   NVSYNRLCG IP GG
Sbjct: 251 SRNLFQFDLSRVEFPKSLTSLDLSHNKIAGSLPEMMTSL-DLQFLNVSYNRLCGKIPVGG 310

Query: 304 ELQRFDVYSYFHNKCLCGAPLPRCK 328
           +LQ FD  SYFHN+CLCGAPL  CK
Sbjct: 311 KLQSFDYDSYFHNRCLCGAPLQSCK 333

BLAST of Lag0011681 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 2.7e-100
Identity = 180/324 (55.56%), Postives = 231/324 (71.30%), Query Frame = 0

Query: 6   LFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEK-- 65
           LFL  FL+  +S  + CN NDK  LL+IKK+ N+PY L SW P+ DCC+W  +ECG+   
Sbjct: 10  LFLFTFLTTCLS-KDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATV 69

Query: 66  SDRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVS 125
           + R+  + I    ++SG IP  VGDLP+L+TL+ RKL NL G + P IAKL NL+ L +S
Sbjct: 70  NHRVTALTIF-SGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLS 129

Query: 126 WTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSF 185
           WTN++G +PDF+  L NLE++ L+FN+L+G++P+SLS LPK+ AL+L RNKLTG IP+SF
Sbjct: 130 WTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESF 189

Query: 186 GYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLS 245
           G        L LSHN+LSG IP SL  +D   IDLSRN L+GDASM+FG++K  W + LS
Sbjct: 190 GSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLS 249

Query: 246 RNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGE 305
           RN+  FD+SKV    +L  LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP GG+
Sbjct: 250 RNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPTGGK 309

Query: 306 LQRFDVYSYFHNKCLCGAPLPRCK 328
           LQ FD YSYFHNKCLCGAPL  CK
Sbjct: 310 LQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Lag0011681 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 364.0 bits (933), Expect = 1.8e-99
Identity = 183/326 (56.13%), Postives = 233/326 (71.47%), Query Frame = 0

Query: 5   TLFLVL-FLSFTISFA-ERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGE 64
           TLFL+L  L  T S A + C+ +DK  LL+IKK+ N+PY L SW P+ DCC+W  +ECG+
Sbjct: 6   TLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGD 65

Query: 65  KS-DRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLD 124
            + +  +T  I  D E+SG IPP VGDLP+L +L+ RKL NL G + P IAKL NL FL 
Sbjct: 66  ATVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLR 125

Query: 125 VSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPD 184
           +SWTN++G VP+FL  L NLEYI L+FN+L+G++P+SLS L KL  L+L RNKLTGPIP+
Sbjct: 126 LSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPE 185

Query: 185 SFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVV 244
           SFG    +   L LSHN+LSG IP SL   D + IDLSRN L+GDAS++FGA K  W V 
Sbjct: 186 SFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVD 245

Query: 245 LSRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQG 304
           +SRN+  FD+SKV  + +L  LD+NHN I G +P + +K +     NVSYNRLCG IP+G
Sbjct: 246 ISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPKG 305

Query: 305 GELQRFDVYSYFHNKCLCGAPLPRCK 328
             +QRFD YS+FHNKCLCGAPLP CK
Sbjct: 306 EYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Lag0011681 vs. ExPASy Swiss-Prot
Match: P35334 (Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris OX=3885 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.0e-86
Identity = 156/327 (47.71%), Postives = 219/327 (66.97%), Query Frame = 0

Query: 5   TLFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCC--TWNSVECGE 64
           ++ LV+ +S   + +E CNP DK+ALL+IKK   +P  L SW P  DCC  TW  V C  
Sbjct: 15  SIILVILVSLRTALSELCNPQDKQALLQIKKDLGNPTTLSSWLPTTDCCNRTWLGVLCDT 74

Query: 65  KSD--RIITIDI-GPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKF 124
            +   R+  +D+ G +     PIP ++ +LP+L  L +  + NLVGP+PPAIAKL  L +
Sbjct: 75  DTQTYRVNNLDLSGHNLPKPYPIPSSLANLPYLNFLYIGGINNLVGPIPPAIAKLTQLHY 134

Query: 125 LDVSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPI 184
           L ++ TN+SG++PDFL  +  L  +  ++N L+GTLP S+S LP LG +  D N+++G I
Sbjct: 135 LYITHTNVSGAIPDFLSQIKTLVTLDFSYNALSGTLPPSISSLPNLGGITFDGNRISGAI 194

Query: 185 PDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWK 244
           PDS+G        + +S N+L+GKIPP+   L+L  +DLSRNMLEGDAS++FG+ K   K
Sbjct: 195 PDSYGSFSKLFTAMTISRNRLTGKIPPTFANLNLAFVDLSRNMLEGDASVLFGSDKNTKK 254

Query: 245 VVLSRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIP 304
           + L++N L+FD+ KV  S +L  LDL +N+I+G LP  +T+L  L S NVS+N LCG IP
Sbjct: 255 IHLAKNSLAFDLGKVGLSKNLNGLDLRNNRIYGTLPQGLTQLKFLQSLNVSFNNLCGEIP 314

Query: 305 QGGELQRFDVYSYFHNKCLCGAPLPRC 327
           QGG L+RFDV SY +NKCLCG+PLP C
Sbjct: 315 QGGNLKRFDVSSYANNKCLCGSPLPSC 341

BLAST of Lag0011681 vs. ExPASy TrEMBL
Match: A0A6J1HMU3 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.1e-143
Identity = 248/320 (77.50%), Postives = 272/320 (85.00%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L  F+ FTISFAE CNPNDKKALL IKKAFN+ Y   SWKPEEDCCTW SVEC E S RI
Sbjct: 13  LFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++DI  D+E+SG IPP VGDLPFLQ+LML KLPNLVGP+ PAIAKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPAIAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQLDRSKLTGLIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRN LEGDASMIFGAH+  WKV LSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGAHRNTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
            FDMSKVVFS SLF LDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQRF
Sbjct: 253 EFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
           DVYSYFHNKCLCGAPL  CK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

BLAST of Lag0011681 vs. ExPASy TrEMBL
Match: A0A6J1EB58 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 PE=4 SV=1)

HSP 1 Score: 518.5 bits (1334), Expect = 2.1e-143
Identity = 247/320 (77.19%), Postives = 272/320 (85.00%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L  FL FTISFAE CNPNDKKALL IKKAFN+ Y   SWKPEEDCCTW SVEC E S RI
Sbjct: 13  LFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEEDCCTWYSVECDENSHRI 72

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++DI  D+E+SG IPP VGDLPFLQ+LML KLPNLVGP+ PA+AKLHNLK LD+SWTNI
Sbjct: 73  ISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPALAKLHNLKHLDISWTNI 132

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L  L YIRL+F+NL+G +P+SLSKLP L  LQLDR+KLTG IP SFG  K
Sbjct: 133 SGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQLDRSKLTGQIPYSFGDFK 192

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           A+   L LSHNKLSGKIPPSL+R+D  S+DLSRN LEGDASMIFG H+  WKV LSRNLL
Sbjct: 193 AKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMIFGVHRHTWKVDLSRNLL 252

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
            FDMSKVVFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVSYNRLCG IP+GGELQRF
Sbjct: 253 EFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVSYNRLCGQIPKGGELQRF 312

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
           DVYSYFHNKCLCGAPL  CK
Sbjct: 313 DVYSYFHNKCLCGAPLESCK 332

BLAST of Lag0011681 vs. ExPASy TrEMBL
Match: A0A6J1HP52 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=4 SV=1)

HSP 1 Score: 474.9 bits (1221), Expect = 2.6e-130
Identity = 229/320 (71.56%), Postives = 261/320 (81.56%), Query Frame = 0

Query: 8   LVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKSDRI 67
           L  F+ FTISFAE CNPNDKKALL IKKAFN+ Y   SWK EEDCCTW SV+C E S RI
Sbjct: 17  LFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKAEEDCCTWYSVQCDENSHRI 76

Query: 68  ITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSWTNI 127
           I++ +  D ++SG IPP VG+LPFLQ L   KLPNLVGP+ PAIAKLHNLK LD+SWT I
Sbjct: 77  ISLTLAVDKQISGQIPPHVGELPFLQYLTFHKLPNLVGPIQPAIAKLHNLKHLDISWTKI 136

Query: 128 SGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFGYLK 187
           SG++PDFL +L NL YI L+F+NL+G +P+SLSKLP L  LQLDR+KLTG IPDSFG  K
Sbjct: 137 SGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSYLQLDRSKLTGQIPDSFGDFK 196

Query: 188 AESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSRNLL 247
           AE+  L LSHNKLSGKIPPSL+++D  +IDLSRN LEGDA MIFGA KKAW V LSRNLL
Sbjct: 197 AENFNLYLSHNKLSGKIPPSLSKVDFSAIDLSRNKLEGDAYMIFGAQKKAWNVDLSRNLL 256

Query: 248 SFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRF 307
           +FDMSKVVFST+LF LD+NHNKIFGGLPPQM+KLS L S NVSYNRLCG IP+G +L+RF
Sbjct: 257 AFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVSYNRLCGQIPKGKKLKRF 316

Query: 308 DVYSYFHNKCLCGAPLPRCK 328
            V SYFHNKCLCG PL  CK
Sbjct: 317 GVDSYFHNKCLCGKPLESCK 335

BLAST of Lag0011681 vs. ExPASy TrEMBL
Match: A0A1W6IYG8 (Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1)

HSP 1 Score: 447.2 bits (1149), Expect = 5.9e-122
Identity = 214/323 (66.25%), Postives = 254/323 (78.64%), Query Frame = 0

Query: 5   TLFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEKS 64
           T F +LF+S +I FAE CNPNDKK LL+IKK+FN+PY+L SWKPEEDCC+W  V+C   S
Sbjct: 7   TFFFLLFISSSICFAELCNPNDKKVLLKIKKSFNNPYILTSWKPEEDCCSWYCVQCHPTS 66

Query: 65  DRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVSW 124
            RII++ I  DD+LSG IPP VGDLPFL+TLML KLPNL GP+P A+A LHNLK LD+SW
Sbjct: 67  HRIISLTIFADDKLSGQIPPVVGDLPFLETLMLHKLPNLTGPIPAAVANLHNLKSLDLSW 126

Query: 125 TNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSFG 184
             +SG VP+FLG+L +L Y+ L+FNNLTG++P+SL+ L +LGAL LDRNKLTGPIP SFG
Sbjct: 127 NGLSGPVPEFLGSLSSLTYLDLSFNNLTGSIPSSLANLQRLGALHLDRNKLTGPIPHSFG 186

Query: 185 YLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLSR 244
           Y K +   L LSHN+LSGKIP SL R++   IDLSRN LEGDAS+IFG +K    V LSR
Sbjct: 187 YFKGKIPYLYLSHNQLSGKIPASLGRVNFNVIDLSRNKLEGDASVIFGGNKTTEIVDLSR 246

Query: 245 NLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGEL 304
           NLL FD+SKVVF  S+ +LDLNHNKIFGG+P ++ +L  L   NVSYNRLCG IP GG L
Sbjct: 247 NLLEFDLSKVVFPRSVTWLDLNHNKIFGGIPEKVVEL-ELQLLNVSYNRLCGRIPVGGRL 306

Query: 305 QRFDVYSYFHNKCLCGAPLPRCK 328
           QRFDVY YFHNKCLCG PL  CK
Sbjct: 307 QRFDVYEYFHNKCLCGKPLGSCK 328

BLAST of Lag0011681 vs. ExPASy TrEMBL
Match: Q7X9Q7 (Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=2 SV=1)

HSP 1 Score: 443.0 bits (1138), Expect = 1.1e-120
Identity = 212/327 (64.83%), Postives = 248/327 (75.84%), Query Frame = 0

Query: 1   MHPSTLFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVEC 60
           MH   L L+LF  FT+SFAE C+PNDK+ LL IKKAFN+PY+L SWKPEEDCCTW  VEC
Sbjct: 1   MHSHNLLLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVEC 60

Query: 61  GEKSDRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFL 120
             KS RII + I  DDELSGPIPP VGDLPFL+ LM  KLPNL GP+PP IAKLHNLK+L
Sbjct: 61  DLKSHRIIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYL 120

Query: 121 DVSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIP 180
           D+SW  +SG +P FLG+L NL+ + L+FN  TG++P+SL+ L +LG L LDRNKLTGPIP
Sbjct: 121 DLSWNGLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIP 180

Query: 181 DSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKV 240
           DSFG  K +   L LSHN+LSGKIP S+ ++D   IDLSRN L GD S+IFG+ K    V
Sbjct: 181 DSFGNFKGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIV 240

Query: 241 VLSRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQ 300
            LSRNLL F++SKVVF  +L +LDLNHNKIFG +P  + KL  L   NVSYN LCG IP 
Sbjct: 241 DLSRNLLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKL-ELQMLNVSYNALCGRIPM 300

Query: 301 GGELQRFDVYSYFHNKCLCGAPLPRCK 328
           GG+LQ FDVYSYFHNKCLCG PL  CK
Sbjct: 301 GGKLQSFDVYSYFHNKCLCGKPLGSCK 326

BLAST of Lag0011681 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 366.7 bits (940), Expect = 1.9e-101
Identity = 180/324 (55.56%), Postives = 231/324 (71.30%), Query Frame = 0

Query: 6   LFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGEK-- 65
           LFL  FL+  +S  + CN NDK  LL+IKK+ N+PY L SW P+ DCC+W  +ECG+   
Sbjct: 10  LFLFTFLTTCLS-KDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATV 69

Query: 66  SDRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLDVS 125
           + R+  + I    ++SG IP  VGDLP+L+TL+ RKL NL G + P IAKL NL+ L +S
Sbjct: 70  NHRVTALTIF-SGQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLS 129

Query: 126 WTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPDSF 185
           WTN++G +PDF+  L NLE++ L+FN+L+G++P+SLS LPK+ AL+L RNKLTG IP+SF
Sbjct: 130 WTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESF 189

Query: 186 GYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVVLS 245
           G        L LSHN+LSG IP SL  +D   IDLSRN L+GDASM+FG++K  W + LS
Sbjct: 190 GSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLS 249

Query: 246 RNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGE 305
           RN+  FD+SKV    +L  LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP GG+
Sbjct: 250 RNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPTGGK 309

Query: 306 LQRFDVYSYFHNKCLCGAPLPRCK 328
           LQ FD YSYFHNKCLCGAPL  CK
Sbjct: 310 LQTFDSYSYFHNKCLCGAPLEICK 330

BLAST of Lag0011681 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 364.0 bits (933), Expect = 1.3e-100
Identity = 183/326 (56.13%), Postives = 233/326 (71.47%), Query Frame = 0

Query: 5   TLFLVL-FLSFTISFA-ERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCTWNSVECGE 64
           TLFL+L  L  T S A + C+ +DK  LL+IKK+ N+PY L SW P+ DCC+W  +ECG+
Sbjct: 6   TLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGD 65

Query: 65  KS-DRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKFLD 124
            + +  +T  I  D E+SG IPP VGDLP+L +L+ RKL NL G + P IAKL NL FL 
Sbjct: 66  ATVNHRVTSLIIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFLR 125

Query: 125 VSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPIPD 184
           +SWTN++G VP+FL  L NLEYI L+FN+L+G++P+SLS L KL  L+L RNKLTGPIP+
Sbjct: 126 LSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIPE 185

Query: 185 SFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWKVV 244
           SFG    +   L LSHN+LSG IP SL   D + IDLSRN L+GDAS++FGA K  W V 
Sbjct: 186 SFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIVD 245

Query: 245 LSRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQG 304
           +SRN+  FD+SKV  + +L  LD+NHN I G +P + +K +     NVSYNRLCG IP+G
Sbjct: 246 ISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPKG 305

Query: 305 GELQRFDVYSYFHNKCLCGAPLPRCK 328
             +QRFD YS+FHNKCLCGAPLP CK
Sbjct: 306 EYIQRFDSYSFFHNKCLCGAPLPSCK 330

BLAST of Lag0011681 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 300.8 bits (769), Expect = 1.3e-81
Identity = 156/327 (47.71%), Postives = 211/327 (64.53%), Query Frame = 0

Query: 1   MHPSTLFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLISWKPEEDCCT-WNSVE 60
           +H S  F +LF++   S++  C  NDK ALL+IKKA  +P +L SW P  DCCT W  VE
Sbjct: 5   VHLSIFFSILFITLPSSYS--CTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVE 64

Query: 61  CGEKSDRIITIDIGPDDELSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPPAIAKLHNLKF 120
           C  +    +++  G   E+SG I   +GDL  L+TL    LP+L G +P  I KL NL  
Sbjct: 65  CTNRRVTGLSVTSG---EVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNT 124

Query: 121 LDVSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQLDRNKLTGPI 180
           L +  T++SG +PD++  L +L ++ L+FN  TG +P SLS++PKL A+Q++ NKLTG I
Sbjct: 125 LYLKHTSLSGPIPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSI 184

Query: 181 PDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLDLFSIDLSRNMLEGDASMIFGAHKKAWK 240
           P+SFG        L LS+NKLSGKIP SL++ D  ++DLS N  EGDA M FG +K   +
Sbjct: 185 PNSFGSFVGNVPNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVR 244

Query: 241 VVLSRNLLSFDMSKVVFSTSLFYLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIP 300
           V LSRN+ +FD+ KV F+ S+  LDL+ N I+G +PP +TKL +L  FNVS N LCG IP
Sbjct: 245 VDLSRNMFNFDLVKVKFARSIVSLDLSQNHIYGKIPPALTKL-HLEHFNVSDNHLCGKIP 304

Query: 301 QGGELQRFDVYSYFHNKCLCGAPLPRC 327
            GG LQ F+  ++ HN CLCG PL  C
Sbjct: 305 SGGLLQTFEPSAFAHNICLCGTPLKAC 325

BLAST of Lag0011681 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 187.2 bits (474), Expect = 2.1e-47
Identity = 121/361 (33.52%), Postives = 182/361 (50.42%), Query Frame = 0

Query: 4   STLFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLI--SWKPEEDCC-TWNSVEC 63
           S+L  V+F+S  IS    C+P D+ AL   K + ++P + I  +W    DCC  W  + C
Sbjct: 10  SSLLAVVFIS-VISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYGISC 69

Query: 64  GEKSDRIITIDIGPDDE------------LSGPIPPAVGDLPFLQTLMLRKLPNLVGPLP 123
              S R+  I +  + E            +SG I PAV DL  L +L+L     + G +P
Sbjct: 70  DPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITGEIP 129

Query: 124 PAIAKLHNLKFLDVSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGAL 183
           P I  L +L+ LD++   I+G +P  +G L  L  + L  N ++G +PASL+ L +L  L
Sbjct: 130 PCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIELKHL 189

Query: 184 QLDRNKLTGPIPDSFGYLKAESIVLDLSHNKLSGKIPPSLTRLD-LFSIDLSRNMLEG-- 243
           +L  N +TG IP  FG LK  S VL L  N+L+G IP S++ ++ L  +DLS+N +EG  
Sbjct: 190 ELTENGITGVIPADFGSLKMLSRVL-LGRNELTGSIPESISGMERLADLDLSKNHIEGPI 249

Query: 244 ---------------DASMIFG-------AHKKAWKVVLSRNLLSFDMSKVVFS-TSLFY 303
                          D + + G       ++       LSRN L   +  V  S T L  
Sbjct: 250 PEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTYLVS 309

Query: 304 LDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRFDVYSYFHNKCLCGAP 324
           LDL+HN + G +P  ++    +   ++S+N+LCG IP G      +  S+  N+CLCG P
Sbjct: 310 LDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCLCGGP 368

BLAST of Lag0011681 vs. TAIR 10
Match: AT5G12940.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 175.6 bits (444), Expect = 6.4e-44
Identity = 114/363 (31.40%), Postives = 174/363 (47.93%), Query Frame = 0

Query: 4   STLFLVLFLSFTISFAERCNPNDKKALLEIKKAFNDPYVLI--SWKPEEDCCTWNSVECG 63
           + + + L LS T+     C P+D+ ALLE +   N+PY+ +  +WK  + C  W  V C 
Sbjct: 12  TNVVVFLLLSTTV---HSCLPSDRAALLEFRAKLNEPYIGVFNTWKGLDCCKGWYGVSCD 71

Query: 64  EKSDRIITIDIGPDDE------------LSGPIPPAVGDLPFLQTLMLRKLPNLVGPLPP 123
             + R+  I +  + E            ++G I P++  L  L  +++     + G +P 
Sbjct: 72  PNTRRVAGITLRGESEDPLFQKAKRSGLMTGSISPSICKLTRLSGIIIADWKGISGVIPS 131

Query: 124 AIAKLHNLKFLDVSWTNISGSVPDFLGTLPNLEYIRLNFNNLTGTLPASLSKLPKLGALQ 183
            I  L  L+ LD+     SG +P  +G L  L+ + L  N+L G +P S+++L  L  L 
Sbjct: 132 CIENLPFLRHLDLVGNKFSGVIPANIGKLLRLKVLNLADNHLYGVIPPSITRLVSLSHLD 191

Query: 184 LDRNKLTGPIPDSFGYLKAESIVLDLSHNKLSGKIPPSLTRL-DLFSIDLSRNMLEGDAS 243
           L  N ++G IP   G LK  S VL LS NK+SG+IP SLTR+  L  ++LS N L G   
Sbjct: 192 LRNNNISGVIPRDIGRLKMVSRVL-LSGNKISGQIPDSLTRIYRLADLELSMNRLTGPIP 251

Query: 244 MIFGAHKKAWKVVLSRNLLSFDMSKVVFSTSL------------------------FYLD 303
             FG       + L  NL+S  +   + ++S+                          LD
Sbjct: 252 ASFGKMSVLATLNLDGNLISGMIPGSLLASSISNLNLSGNLITGSIPNTFGPRSYFTVLD 311

Query: 304 LNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPQGGELQRFDVYSYFHNKCLCGAPLP 328
           L +N++ G +P  +T  S +   +VS+N LCG IP G      D  S+ +N CLCG PL 
Sbjct: 312 LANNRLQGPIPASITAASFIGHLDVSHNHLCGKIPMGSPFDHLDATSFAYNACLCGKPLG 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022966387.14.3e-14377.50polygalacturonase inhibitor-like [Cucurbita maxima][more]
XP_022925061.14.3e-14377.19polygalacturonase inhibitor-like [Cucurbita moschata][more]
KAG6595655.15.6e-14377.19Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023517556.18.1e-14276.25polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo][more]
KAG6595656.17.6e-13271.88Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
Q050911.8e-10760.31Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1[more]
A7PW812.9e-10259.08Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1[more]
Q9M5J92.7e-10055.56Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1[more]
Q9M5J81.8e-9956.13Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2[more]
P353345.0e-8647.71Polygalacturonase inhibitor 1 OS=Phaseolus vulgaris OX=3885 GN=PGIP1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HMU32.1e-14377.50polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=... [more]
A0A6J1EB582.1e-14377.19polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 P... [more]
A0A6J1HP522.6e-13071.56polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=... [more]
A0A1W6IYG85.9e-12266.25Pgip1 OS=Momordica charantia OX=3673 GN=LOC111019559 PE=2 SV=1[more]
Q7X9Q71.1e-12064.83Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=... [more]
Match NameE-valueIdentityDescription
AT5G06860.11.9e-10155.56polygalacturonase inhibiting protein 1 [more]
AT5G06870.11.3e-10056.13polygalacturonase inhibiting protein 2 [more]
AT3G12145.11.3e-8147.71Leucine-rich repeat (LRR) family protein [more]
AT3G12610.12.1e-4733.52Leucine-rich repeat (LRR) family protein [more]
AT5G12940.16.4e-4431.40Leucine-rich repeat (LRR) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013210Leucine-rich repeat-containing N-terminal, plant-typePFAMPF08263LRRNT_2coord: 24..60
e-value: 1.5E-10
score: 41.1
IPR032675Leucine-rich repeat domain superfamilyGENE3D3.80.10.10Ribonuclease Inhibitorcoord: 21..327
e-value: 1.9E-87
score: 295.3
IPR001611Leucine-rich repeatPFAMPF00560LRR_1coord: 192..209
e-value: 0.87
score: 10.4
IPR001611Leucine-rich repeatPFAMPF13855LRR_8coord: 116..175
e-value: 1.1E-6
score: 28.3
NoneNo IPR availablePANTHERPTHR48059:SF10POLYGALACTURONASE INHIBITOR 2coord: 6..326
NoneNo IPR availablePANTHERPTHR48059POLYGALACTURONASE INHIBITOR 1coord: 6..326
NoneNo IPR availableSUPERFAMILY52058L domain-likecoord: 21..326

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0011681.1Lag0011681.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding
molecular_function GO:0016740 transferase activity