Lag0010514 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0010514 vs. NCBI nr
Match: XP_022131662.1 (uncharacterized protein LOC111004787 [Momordica charantia])

HSP 1 Score: 179.9 bits (455), Expect = 3.3e-41
Identity = 83/172 (48.26%), Postives = 111/172 (64.53%), Query Frame = 0

Query: 110 ARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQLLAS 169
           ARFWWGS++  KK+ W  W  +CLPKELGGLNFRDL  FN+AL+AKQVWR+     +L S
Sbjct: 48  ARFWWGSSNDTKKLHWMSWERMCLPKELGGLNFRDLEGFNQALVAKQVWRVLQNPNILVS 107

Query: 170 RVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLWIPK 229
           RV+  +Y H + +LQA    N S FW G +W RDLL+ G+R RVGNG+T + F+D WIP+
Sbjct: 108 RVLKAKYFHDSLVLQATHLRNQSYFWKGFIWGRDLLIKGLRSRVGNGSTINIFSDPWIPR 167

Query: 230 EVTLMPMQIHEAIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATIPI 282
             +  P  I     P  ++VAD I P+  WD+  +     EED  LI ++P+
Sbjct: 168 PYSFRP--ITAPFGPYDVKVADLINPNGQWDVHLISYIFCEEDRDLILSMPV 217

BLAST of Lag0010514 vs. NCBI nr
Match: XP_030497600.1 (uncharacterized protein LOC115713257 [Cannabis sativa])

HSP 1 Score: 151.0 bits (380), Expect = 1.7e-32
Identity = 74/173 (42.77%), Postives = 97/173 (56.07%), Query Frame = 0

Query: 109  MARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQLLA 168
            MARFWWGS++  KKI WK W  LC  K  GGL FR    FN+A LAKQ WR+F     L 
Sbjct: 836  MARFWWGSSTDNKKIHWKNWKSLCTSKRDGGLGFRSFVHFNQAFLAKQAWRIFQTPNSLL 895

Query: 169  SRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLWIP 228
            SRV+ GRY HQN  + A +    S+ W G+VW R+LL  G+  ++G+GT  +  +D WIP
Sbjct: 896  SRVLKGRYYHQNDFMTAKVSGLSSLTWQGIVWGRELLSKGLIIKIGDGTGVNCAHDSWIP 955

Query: 229  KEVTLMPMQIHEAIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATIPI 282
                  P++   +       VAD+IT +  WDL  L +     D   I TIP+
Sbjct: 956  GNEYFKPLRFTGSC---SNLVADYITDTREWDLELLHNDFSPADIDRILTIPL 1005

BLAST of Lag0010514 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 149.8 bits (377), Expect = 3.7e-32
Identity = 70/161 (43.48%), Postives = 95/161 (59.01%), Query Frame = 0

Query: 121  KKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQLLASRVIHGRYAHQN 180
            +K+ W KW  +C PKE GGLNFRDL  FN+AL+AK VWR      LL S+V+  +Y    
Sbjct: 927  RKLHWMKWGRMCYPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDT 986

Query: 181  QLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLWIPKEVTLMPMQIHE 240
             LLQA   +  S FW G +W RDLL+ G+R RVGNG+T   F+D W+P+  T  P++ + 
Sbjct: 987  SLLQASNNSKSSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNN 1046

Query: 241  AIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATIPI 282
              +     VA FIT    WD++ +      ED  LI ++PI
Sbjct: 1047 GAL--DTTVASFITADGNWDVTSISHSFCNEDRDLILSMPI 1085

BLAST of Lag0010514 vs. NCBI nr
Match: XP_022568650.1 (uncharacterized protein LOC106442391 [Brassica napus])

HSP 1 Score: 148.7 bits (374), Expect = 8.2e-32
Identity = 71/181 (39.23%), Postives = 104/181 (57.46%), Query Frame = 0

Query: 107 STMARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQL 166
           S M  FWW S + KKKI W  W  LC  KELGGL F+D+  FN+ALLAKQ WR+++  + 
Sbjct: 669 SAMVEFWWSSGNNKKKIPWVAWQKLCKDKELGGLGFKDIEKFNQALLAKQAWRIWSNPES 728

Query: 167 LASRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLW 226
           L +R++  RY  +   L+  I T  S  W  ++  R+LL  G+ H++G+G TT  ++D W
Sbjct: 729 LVARILKHRYFARTDFLECSIGTRPSFAWRSIIHGRELLSQGLYHKIGSGETTRLWSDNW 788

Query: 227 IPKEVTLMPMQIHEAIVPEGLEVADFITPSLG-WDLSKLRSFVVEEDTRLIATIPIIVAE 286
           +       PM   +AIV   L V+D I   +G W + ++R  +VEED   +   PI +A 
Sbjct: 789 LVDGRARPPMYRQDAIVDLTLTVSDLIDSRVGSWSVPRIRELIVEEDVERVLQTPIDLAR 848

BLAST of Lag0010514 vs. NCBI nr
Match: XP_013617205.1 (PREDICTED: uncharacterized protein LOC106323665 [Brassica oleracea var. oleracea])

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-31
Identity = 71/179 (39.66%), Postives = 103/179 (57.54%), Query Frame = 0

Query: 107 STMARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQL 166
           S M  FWW S + KKKI W  W  LC  KELGGL F+D+  FN+ALLAKQ WR+++  + 
Sbjct: 265 SAMVEFWWSSGNNKKKIPWVAWQKLCKDKELGGLGFKDIEKFNQALLAKQAWRIWSNPES 324

Query: 167 LASRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLW 226
           L +R++  RY  +   L+  I T  S  W  ++  R+LL  G+ H++G+G TT  ++D W
Sbjct: 325 LVARILKHRYFARTDFLECSIGTRPSFAWRSIIHGRELLSQGLYHKIGSGETTRLWSDNW 384

Query: 227 IPKEVTLMPMQIHEAIVPEGLEVADFITPSLG-WDLSKLRSFVVEEDTRLIATIPIIVA 285
           +       PM   +AIV   L V+D I   +G W + ++R  +VEED   +   PI +A
Sbjct: 385 LVDGRARPPMYRQDAIVDLTLTVSDLIDSRVGSWSVPRIRELIVEEDVERVLQTPIDLA 443

BLAST of Lag0010514 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 4.7e-22
Identity = 56/140 (40.00%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 100 SILLCN--ISTMARFWWGSTSTKKKIQWKKWADLCLPKE-LGGLNFRDLASFNKALLAKQ 159
           S LLC    S M  FWW S   K+KI W  W  LC  KE  GGL FRDL  FN+ALLAKQ
Sbjct: 14  SKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQ 73

Query: 160 VWRLFTILQLLASRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNG 219
            +R+      L SR++  RY   + +++  + T  S  W  ++  R+LL  G+   +G+G
Sbjct: 74  SFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDG 133

Query: 220 TTTDFFNDLWIPKEVTLMPM 237
             T  + D WI  E  L P+
Sbjct: 134 IHTKVWLDRWIMDETPLPPL 153

BLAST of Lag0010514 vs. ExPASy TrEMBL
Match: A0A6J1BRN0 (uncharacterized protein LOC111004787 OS=Momordica charantia OX=3673 GN=LOC111004787 PE=4 SV=1)

HSP 1 Score: 179.9 bits (455), Expect = 1.6e-41
Identity = 83/172 (48.26%), Postives = 111/172 (64.53%), Query Frame = 0

Query: 110 ARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQLLAS 169
           ARFWWGS++  KK+ W  W  +CLPKELGGLNFRDL  FN+AL+AKQVWR+     +L S
Sbjct: 48  ARFWWGSSNDTKKLHWMSWERMCLPKELGGLNFRDLEGFNQALVAKQVWRVLQNPNILVS 107

Query: 170 RVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLWIPK 229
           RV+  +Y H + +LQA    N S FW G +W RDLL+ G+R RVGNG+T + F+D WIP+
Sbjct: 108 RVLKAKYFHDSLVLQATHLRNQSYFWKGFIWGRDLLIKGLRSRVGNGSTINIFSDPWIPR 167

Query: 230 EVTLMPMQIHEAIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATIPI 282
             +  P  I     P  ++VAD I P+  WD+  +     EED  LI ++P+
Sbjct: 168 PYSFRP--ITAPFGPYDVKVADLINPNGQWDVHLISYIFCEEDRDLILSMPV 217

BLAST of Lag0010514 vs. ExPASy TrEMBL
Match: A0A803PTB0 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 9.4e-34
Identity = 74/170 (43.53%), Postives = 97/170 (57.06%), Query Frame = 0

Query: 110 ARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQLLAS 169
           ARFWWGS+  KKK  W  W+ LCLPKE GGL F+DL  FNKALLAKQVWR+      L  
Sbjct: 559 ARFWWGSSKEKKKTHWGAWSKLCLPKEKGGLGFKDLELFNKALLAKQVWRVVRNPMSLVG 618

Query: 170 RVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLWIPK 229
           +V+   Y   + +L A   +  S  W GL+W R+++ AG R RVG+G   D  ND W+P+
Sbjct: 619 KVLKSSYFSHSSILDAKKGSCSSAVWRGLLWGREIVEAGSRWRVGSGENIDIINDRWLPR 678

Query: 230 EVTLMPMQIHEAIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATI 280
            V   P  I    +PEG +V D    +  WD   ++    E+D  LI +I
Sbjct: 679 PVRFRP--IINPDIPEGTKVVDLRLANGDWDAEFIKEIFCEDDAELIMSI 726

BLAST of Lag0010514 vs. ExPASy TrEMBL
Match: A0A803PIB6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 151.0 bits (380), Expect = 8.0e-33
Identity = 74/173 (42.77%), Postives = 97/173 (56.07%), Query Frame = 0

Query: 109  MARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQLLA 168
            MARFWWGS++  KKI WK W  LC  K  GGL FR    FN+A LAKQ WR+F     L 
Sbjct: 1296 MARFWWGSSTDNKKIHWKNWKSLCTSKRDGGLGFRSFVHFNQAFLAKQAWRIFQTPNSLL 1355

Query: 169  SRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLWIP 228
            SRV+ GRY HQN  + A +    S+ W G+VW R+LL  G+  ++G+GT  +  +D WIP
Sbjct: 1356 SRVLKGRYYHQNDFMTAKVSGLSSLTWQGIVWGRELLSKGLIIKIGDGTGVNCAHDSWIP 1415

Query: 229  KEVTLMPMQIHEAIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATIPI 282
                  P++   +       VAD+IT +  WDL  L +     D   I TIP+
Sbjct: 1416 GNEYFKPLRFTGSC---SNLVADYITDTREWDLELLHNDFSPADIDRILTIPL 1465

BLAST of Lag0010514 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 1.8e-32
Identity = 70/161 (43.48%), Postives = 95/161 (59.01%), Query Frame = 0

Query: 121  KKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQLLASRVIHGRYAHQN 180
            +K+ W KW  +C PKE GGLNFRDL  FN+AL+AK VWR      LL S+V+  +Y    
Sbjct: 927  RKLHWMKWGRMCYPKECGGLNFRDLEGFNQALVAKHVWRFLQHPNLLVSKVLKHKYFKDT 986

Query: 181  QLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLWIPKEVTLMPMQIHE 240
             LLQA   +  S FW G +W RDLL+ G+R RVGNG+T   F+D W+P+  T  P++ + 
Sbjct: 987  SLLQASNNSKSSYFWKGFLWGRDLLVKGLRLRVGNGSTIKAFSDPWLPRPTTFKPLRFNN 1046

Query: 241  AIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATIPI 282
              +     VA FIT    WD++ +      ED  LI ++PI
Sbjct: 1047 GAL--DTTVASFITADGNWDVTSISHSFCNEDRDLILSMPI 1085

BLAST of Lag0010514 vs. ExPASy TrEMBL
Match: A0A803PKJ2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 148.7 bits (374), Expect = 4.0e-32
Identity = 75/175 (42.86%), Postives = 91/175 (52.00%), Query Frame = 0

Query: 107 STMARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQL 166
           S MARFWWGS++  KKI WK W  LC  K  GGL FR    FN+A LAKQ WR+F     
Sbjct: 331 SMMARFWWGSSTDNKKIHWKSWKSLCTSKGDGGLGFRSFVHFNQAFLAKQAWRVFQNPHS 390

Query: 167 LASRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDLW 226
           L SRV+ GRY H N  L A      S+ W G++W R+LL  G+R ++G GT     ND W
Sbjct: 391 LLSRVLKGRYYHHNDFLSAKASGLSSLTWQGIIWGRELLQQGLRIKIGTGTAVSCANDSW 450

Query: 227 IPKEVTLMPMQIHEAIVPEGLEVADFITPSLGWDLSKLRSFVVEEDTRLIATIPI 282
           IP      P Q      P    VA +IT +  W+   L       D   I TIP+
Sbjct: 451 IPGHDFFKPFQF---TGPSNNWVAQYITDAREWNWELLHRDFSPVDVEKILTIPL 502

BLAST of Lag0010514 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 106.7 bits (265), Expect = 3.3e-23
Identity = 56/140 (40.00%), Postives = 75/140 (53.57%), Query Frame = 0

Query: 100 SILLCN--ISTMARFWWGSTSTKKKIQWKKWADLCLPKE-LGGLNFRDLASFNKALLAKQ 159
           S LLC    S M  FWW S   K+KI W  W  LC  KE  GGL FRDL  FN+ALLAKQ
Sbjct: 14  SKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRDLGWFNQALLAKQ 73

Query: 160 VWRLFTILQLLASRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNG 219
            +R+      L SR++  RY   + +++  + T  S  W  ++  R+LL  G+   +G+G
Sbjct: 74  SFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGRELLSRGLLRTIGDG 133

Query: 220 TTTDFFNDLWIPKEVTLMPM 237
             T  + D WI  E  L P+
Sbjct: 134 IHTKVWLDRWIMDETPLPPL 153

BLAST of Lag0010514 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 97.4 bits (241), Expect = 2.0e-20
Identity = 56/181 (30.94%), Postives = 88/181 (48.62%), Query Frame = 0

Query: 106 ISTMARFWWGSTSTKKKIQWKKWADLCLPKELGGLNFRDLASFNKALLAKQVWRLFTILQ 165
           IS +A FWW +    K + WK W  L   K  GG+ F+D+ +FN ALL KQ+WR+ +  +
Sbjct: 22  ISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDIEAFNLALLGKQMWRMLSRPE 81

Query: 166 LLASRVIHGRYAHQNQLLQAPIKTNCSVFWWGLVWARDLLLAGMRHRVGNGTTTDFFNDL 225
            L ++V   RY H++  L AP+ +  S  W  +  ++++L  G R  VGNG     +   
Sbjct: 82  SLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEILRQGARAVVGNGEDIIIWRHK 141

Query: 226 WI---PKEVTLMPMQI---HEAIVPEGLEVADFITPS-LGWDLSKLRSFVVEEDTRLIAT 280
           W+   P    L   ++     A V   L+V+D I  S   W    +     E + +LI  
Sbjct: 142 WLDSKPASAALRMQRVPPQEYASVSSILKVSDLIDESGREWRKDVIEMLFPEVERKLIGE 201