Lag0009377 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009377
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionisocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like
Locationchr9: 38660600 .. 38663720 (+)
RNA-Seq ExpressionLag0009377
SyntenyLag0009377
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGCCCGCCGCGCCTTTCCGATCATACGCCGCCTCGCCTCCCGTTCCCGCCTCTCCCCTTTCACTCTCTTCTCCGACGCCCGATCCGTCACCTACATGCACAGGCCCGGCGACGGAGCCCCGCGCCCGGTCACTCTCATTCCCGGAGACGGAATCGGTCCTCTGGTTACTAATGCGGTCGAGCAAGTGATGGAGGCGTTGCACGCTCCTGTCTACTTCGAGCGCTTCGATGTCCATGGCGATATGAAGCAGGTTCCTCGCGAGGTAATGGAGTCTATTTGGAGGAATAAGGTTTGTTTGAAAGGCGGCCTGAGAACTCCGATGGGCGGCGGTGTTAGTTCACTCAATGTGCAACTGAGGAAGGAGCTCGACTTGTATGCCTCGCTTGTTAATTGTTTCAATTTCCCTGGATTGCCGACGCGTCATGAGAATGTCGATATCGTCGTCATTAGGGAGAATACGGAGGGTGAATACTCTGGCCTCGAGCATGAGGTCATTCCTGGTGTCGTCGAAAGCCTCAAGGTAATATATAATTTGTATATGTCTAGTCTGGTGATCGATTGATTTTATTTATGGCTGTTCTGTTTGGATTGATGTAGTTGATTTTTTATATAGTTCTGAATTTTATGTTTTAGGGCTCTGTTTCTGGTTCTCTGATTTATGGAAGTTATCTCTGTTTCACTTTTACATATTATGGACTTATACACATCGTTCTTGAAATGAACTAAACGAACAAAGCTGTGAAATAAATGCTGCATTGAATTAACATTAATTGTTTCTTCGATTAGTTTCAATATTTCTTAGGTCTGTTGGATGAGATCAAGTCATCAATAGTATTTTAGTTAGAACCTGTGAATGGAGATGAATGGGTTATCATGTTAGAGTTGCTGATGCAATATCTTAGCCTATATGAAATCTAAATCCCCCCATTCTGCAATAATTCAAGTATCTTACACGCAGATATATAGACTCTTATGCTCAATGGGTCATACTCTCGATTGGTCAATTGCATTTTCTCTGATTCCTCATTTCAATGCAGTAACCTGAAATATAGCCTGAATTACTGTAGGTGATTACGCAGTTTTCCTCAGATCGCATTGCCAGATATGCATTTGAGTATGCTTACCTAAACAACAGGAAGAAAGTGACGGCTGTGCACAAAGCCAACATCATGAAACTTGCAGATGGTTTGTTTCTGGAGTCTTGCAGGGAAGTGGCAACCCAATATCCTAGTATCCAATACAATGAGGTTATTGTTGACAACTGTTGTATGCAGCTTGTTTCAAAACCTGAGCAATTTGATGTAATGGTATGTAGCTTTCTAAGCTGATATTTCTTCCCTTCGGTGTACTTTGGTCTTTTTTTCTCCTTTTGTCTTCGTTTATTATTATTATTTATTATATTTTTTTTTTTTTTCCCCACCACCACAAGCCAGAATGAAAACTATATCAATTTTCTGTGGTCCTTTAGTGGTTATGAACTCTAGAGTCTCAGACAAGCTGTATACTTCAGCTCTCTGGTCCCTGAAAAATTCTTCTTCCTACTTTTGAAACTTGAATCAAGGGGCAATCTGGTTCAAGCTTGCAGTACCTGCACATTAGTTTCTTTGGACATGAATTTAAATGGCATGTATATAGTCCTCGAGATGTTAGACAAGTCGATCTAATTTGTACAAACAAGTTTGGGTCAGTTTTGTTGCTGTGTCATATAAGTTTCAATTGAAATGGAGGGCATACCTGTTAAAATAAAATATTAACATTAGCTCTTGCAGAAGCATATTTTAAAGTGTCTATGCAAATGTATTAAATGTATATGCTAGTTATTCAAGATATGACTTGGAGATGGTAAATATTTCAGAGAGGATTTAGAAATACCTTTTCCTTGAATTACATTCCTTGGGCTCCAAGTAATTCGGGAATTCCTTTAGATAGGGTTGGATTTTCAAATAAATATTTAGGACATTTAGAATTCAATAATTACCTAGAAAAATATCTACTCCAAGGATTTCTTGCCTATAAACCTAAGCTTGGTCGCGGCATACAAGGTAAGTGAATGAGGAAGCAAACGAGTGAGGATTAGAGGGTGAGAAAGTGCCTTCTCAATTAGTAGCGTCTTTCCTCATATTTTCTTAGTAAAAGAGTGTTGGTCTCTTCTTTTTGTGCTAATCTCTTCAACAATATATGATGGAAGCATGTAAAGGTGAGTAACCACCCTGAATTAACCTTGGTAGTCAATAGAGAATAATGTAAATATTACTAAGTTATTTAAGTCATGGTCACCAACTATCCGTTATTTATTATATTCTAACAGTTTTTCTTGATAACCAAATGTAGTAGGGTCAAACAATTGTAAGCTACCAAAAACTCATAAATGTAAACAAGAAAGGGTACGAGTTCCAATTCATCATGGTTTTGTTTAATGCTTCTCTCTCTGTTTTTTACCCCCTCAGATTAAGTTAGTCACTCTCTCCTATCAAACGTTTGTCTATTTTCTTGATGCTTATATTTTGCTTATTCACGTCATTTGACAAAAAAAAAAATCACATAAGTGTATGAGTTCCAATTCATCTTCTTCGTTTTGTTGATCCTCTATCCTCTCAAGGCCTTTTTATGTTTAAATCTTGCTAACAATGCCTCTTAATTCTTTGGGTCAGGTGACACCCAACCTTTATGGCAATCTAGTGGCAAACACTGCAGCTGGAATTGCCGGAGGAACTGGTCTCATGCCAGGAGGTATAATTACTTACTATCCACTCTTTTAAGTCGGTCCAATTACTGCTGAGCTATGCTTACTTCAACAAAGTGTATCTGAGTTCGTATTTGGTTAATTTGATTATACTGCAGGGAACGTGGGGGCAGATCATGCGATATTTGAGCAAGGAGCTTCAGCAGGGAACGTGGGGAATGAGAAAATAGTGGAGAAAAAGAAAGCGAACCCAGTTGCTTTGCTGCTGTCTTCAGCCATGATGCTGAGGCACCTGCAGTTTCCTTCGTTCGCTGATAGACTTGAGAGTGCTGTGAAACGTGTGATCATGGAGGACAAGTGCAGGACCAAGGATCTCGGTGGCAACTGCACTACCCAAGATGTCGTCGATGCTGTCATTGCCTTTTTGGACTGA

mRNA sequence

ATGGCCGCCCGCCGCGCCTTTCCGATCATACGCCGCCTCGCCTCCCGTTCCCGCCTCTCCCCTTTCACTCTCTTCTCCGACGCCCGATCCGTCACCTACATGCACAGGCCCGGCGACGGAGCCCCGCGCCCGGTCACTCTCATTCCCGGAGACGGAATCGGTCCTCTGGTTACTAATGCGGTCGAGCAAGTGATGGAGGCGTTGCACGCTCCTGTCTACTTCGAGCGCTTCGATGTCCATGGCGATATGAAGCAGGTTCCTCGCGAGGTAATGGAGTCTATTTGGAGGAATAAGGTTTGTTTGAAAGGCGGCCTGAGAACTCCGATGGGCGGCGGTGTTAGTTCACTCAATGTGCAACTGAGGAAGGAGCTCGACTTGTATGCCTCGCTTGTTAATTGTTTCAATTTCCCTGGATTGCCGACGCGTCATGAGAATGTCGATATCGTCGTCATTAGGGAGAATACGGAGGGTGAATACTCTGGCCTCGAGCATGAGGTCATTCCTGGTGTCGTCGAAAGCCTCAAGGTGATTACGCAGTTTTCCTCAGATCGCATTGCCAGATATGCATTTGAGTATGCTTACCTAAACAACAGGAAGAAAGTGACGGCTGTGCACAAAGCCAACATCATGAAACTTGCAGATGGTTTGTTTCTGGAGTCTTGCAGGGAAGTGGCAACCCAATATCCTAGTATCCAATACAATGAGGTTATTGTTGACAACTGTTGTATGCAGCTTGTTTCAAAACCTGAGCAATTTGATGTAATGGTGACACCCAACCTTTATGGCAATCTAGTGGCAAACACTGCAGCTGGAATTGCCGGAGGAACTGGTCTCATGCCAGGAGGGAACGTGGGGGCAGATCATGCGATATTTGAGCAAGGAGCTTCAGCAGGGAACGTGGGGAATGAGAAAATAGTGGAGAAAAAGAAAGCGAACCCAGTTGCTTTGCTGCTGTCTTCAGCCATGATGCTGAGGCACCTGCAGTTTCCTTCGTTCGCTGATAGACTTGAGAGTGCTGTGAAACGTGTGATCATGGAGGACAAGTGCAGGACCAAGGATCTCGGTGGCAACTGCACTACCCAAGATGTCGTCGATGCTGTCATTGCCTTTTTGGACTGA

Coding sequence (CDS)

ATGGCCGCCCGCCGCGCCTTTCCGATCATACGCCGCCTCGCCTCCCGTTCCCGCCTCTCCCCTTTCACTCTCTTCTCCGACGCCCGATCCGTCACCTACATGCACAGGCCCGGCGACGGAGCCCCGCGCCCGGTCACTCTCATTCCCGGAGACGGAATCGGTCCTCTGGTTACTAATGCGGTCGAGCAAGTGATGGAGGCGTTGCACGCTCCTGTCTACTTCGAGCGCTTCGATGTCCATGGCGATATGAAGCAGGTTCCTCGCGAGGTAATGGAGTCTATTTGGAGGAATAAGGTTTGTTTGAAAGGCGGCCTGAGAACTCCGATGGGCGGCGGTGTTAGTTCACTCAATGTGCAACTGAGGAAGGAGCTCGACTTGTATGCCTCGCTTGTTAATTGTTTCAATTTCCCTGGATTGCCGACGCGTCATGAGAATGTCGATATCGTCGTCATTAGGGAGAATACGGAGGGTGAATACTCTGGCCTCGAGCATGAGGTCATTCCTGGTGTCGTCGAAAGCCTCAAGGTGATTACGCAGTTTTCCTCAGATCGCATTGCCAGATATGCATTTGAGTATGCTTACCTAAACAACAGGAAGAAAGTGACGGCTGTGCACAAAGCCAACATCATGAAACTTGCAGATGGTTTGTTTCTGGAGTCTTGCAGGGAAGTGGCAACCCAATATCCTAGTATCCAATACAATGAGGTTATTGTTGACAACTGTTGTATGCAGCTTGTTTCAAAACCTGAGCAATTTGATGTAATGGTGACACCCAACCTTTATGGCAATCTAGTGGCAAACACTGCAGCTGGAATTGCCGGAGGAACTGGTCTCATGCCAGGAGGGAACGTGGGGGCAGATCATGCGATATTTGAGCAAGGAGCTTCAGCAGGGAACGTGGGGAATGAGAAAATAGTGGAGAAAAAGAAAGCGAACCCAGTTGCTTTGCTGCTGTCTTCAGCCATGATGCTGAGGCACCTGCAGTTTCCTTCGTTCGCTGATAGACTTGAGAGTGCTGTGAAACGTGTGATCATGGAGGACAAGTGCAGGACCAAGGATCTCGGTGGCAACTGCACTACCCAAGATGTCGTCGATGCTGTCATTGCCTTTTTGGACTGA

Protein sequence

MAARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQDVVDAVIAFLD
Homology
BLAST of Lag0009377 vs. NCBI nr
Match: XP_022971037.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 693.0 bits (1787), Expect = 1.4e-195
Identity = 351/376 (93.35%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAARRAFPIIRRLA----SRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MAAR AFPII RLA    SR +LSPF LFSDARSVTYM RPGDGAPR VTLIPGDGIGPL
Sbjct: 1   MAARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEALHAPVYFER+DVHG+MKQVPREVMESI++NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGL TRHENVDI VIRENTEGEYSGLEHEVIPGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYSGLEHEVIPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYP IQYNEV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVGADHAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVIMEDKCRTKDLGG
Sbjct: 301 AGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQDVVDAVIAFLD
Sbjct: 361 NCTTQDVVDAVIAFLD 376

BLAST of Lag0009377 vs. NCBI nr
Match: XP_023534325.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 691.8 bits (1784), Expect = 3.2e-195
Identity = 350/376 (93.09%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAARRAFPIIRRLA----SRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MAAR AFPII RLA    SR +LSPF LFSDARSVTYM RPGDGAPR VTLIPGDGIGPL
Sbjct: 1   MAARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEALHAPVYFER+DVHG+MKQVPREVMESI++NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGL TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYP IQYNEV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVGADHAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVIMEDKCRTKDLGG
Sbjct: 301 AGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQDVVDAVIAFLD
Sbjct: 361 NCTTQDVVDAVIAFLD 376

BLAST of Lag0009377 vs. NCBI nr
Match: KAG6604970.1 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 691.4 bits (1783), Expect = 4.2e-195
Identity = 349/376 (92.82%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAARRAFPIIRRLA----SRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MAAR AFPII RLA    SR +LSPF LFSDARSVTYM RPGDGAPR VTLIPGDGIGPL
Sbjct: 1   MAARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEALHAPVYFER+DVHG+MKQ+PREVMESI++NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEALHAPVYFERYDVHGNMKQIPREVMESIFKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGL TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYP IQYNEV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVGADHAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVIMEDKCRTKDLGG
Sbjct: 301 AGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQDVVDAVIAFLD
Sbjct: 361 NCTTQDVVDAVIAFLD 376

BLAST of Lag0009377 vs. NCBI nr
Match: KAG7035004.1 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 691.0 bits (1782), Expect = 5.5e-195
Identity = 349/376 (92.82%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAARRAFPIIRRLA----SRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MAAR AFPII RLA    SR +LSPF LFSDARSVTYM RPGDGAPR VTLIPGDGIGPL
Sbjct: 1   MAARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEALHAPVYFER+DVHG+MKQVPREVMESI++NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGL TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYP IQYNEV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVGADHAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALL+SSAMMLRHLQFPSFADRLE+AVKRVIMEDKCRTKDLGG
Sbjct: 301 AGNVGNEKIVEQKKANPVALLMSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQDVVDAVIAFLD
Sbjct: 361 NCTTQDVVDAVIAFLD 376

BLAST of Lag0009377 vs. NCBI nr
Match: XP_022948062.1 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 686.8 bits (1771), Expect = 1.0e-193
Identity = 348/376 (92.55%), Postives = 361/376 (96.01%), Query Frame = 0

Query: 1   MAARRAFPIIRRLA----SRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MAAR AF II RLA    SR +LSPF LFSDARSVTYM RPGDGAPR VTLIPGDGIGPL
Sbjct: 1   MAARSAFHIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEALHAPVYFER+DVHG+MK+VPREVMESI++NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEALHAPVYFERYDVHGNMKRVPREVMESIFKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGL TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYP IQYNEV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVGADHAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVIMEDKCRTKDLGG
Sbjct: 301 AGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQDVVDAVIAFLD
Sbjct: 361 NCTTQDVVDAVIAFLD 376

BLAST of Lag0009377 vs. ExPASy Swiss-Prot
Match: Q8LFC0 (Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH1 PE=1 SV=2)

HSP 1 Score: 615.9 bits (1587), Expect = 2.9e-175
Identity = 306/370 (82.70%), Postives = 338/370 (91.35%), Query Frame = 0

Query: 3   ARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVE 62
           +RR+  +++ LA  +  S        RSVTYM RPGDGAPR VTLIPGDGIGPLVTNAVE
Sbjct: 2   SRRSLTLLKNLARNANGSGI----QTRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTNAVE 61

Query: 63  QVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRK 122
           QVMEA+HAP++FE++DVHG+M +VP EVMESI +NKVCLKGGL+TP+GGGVSSLNVQLRK
Sbjct: 62  QVMEAMHAPIFFEKYDVHGEMSRVPPEVMESIRKNKVCLKGGLKTPVGGGVSSLNVQLRK 121

Query: 123 ELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSS 182
           ELDL+ASLVNCFN PGLPTRHENVDIVVIRENTEGEY+GLEHEV+PGVVESLKVIT+F S
Sbjct: 122 ELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCS 181

Query: 183 DRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCC 242
           +RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA +YPSI YNE+IVDNCC
Sbjct: 182 ERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPSITYNEIIVDNCC 241

Query: 243 MQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGN 302
           MQLV+KPEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVGADHA+FEQGASAGNVG 
Sbjct: 242 MQLVAKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASAGNVGK 301

Query: 303 EKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQD 362
           +KIV + KANPVALLLSSAMMLRHLQFPSFADRLE+AVK+VI E KCRTKDLGG  TTQ+
Sbjct: 302 DKIVLENKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIAEGKCRTKDLGGTSTTQE 361

Query: 363 VVDAVIAFLD 373
           VVDAVIA LD
Sbjct: 362 VVDAVIAKLD 367

BLAST of Lag0009377 vs. ExPASy Swiss-Prot
Match: O81796 (Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH3 PE=1 SV=1)

HSP 1 Score: 602.8 bits (1553), Expect = 2.6e-171
Identity = 303/370 (81.89%), Postives = 337/370 (91.08%), Query Frame = 0

Query: 3   ARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVE 62
           ARR+  I  RL +    SPFT  S  RS+TYM RPGDGAPR VTLIPGDGIGPLVT AVE
Sbjct: 2   ARRSVSIFNRLLANPP-SPFTSLS--RSITYMPRPGDGAPRTVTLIPGDGIGPLVTGAVE 61

Query: 63  QVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRK 122
           QVMEA+HAPV+FER++V G+M++VP EV+ES+ RNKVCLKGGL TP+GGGVSSLN+QLRK
Sbjct: 62  QVMEAMHAPVHFERYEVLGNMRKVPEEVIESVKRNKVCLKGGLATPVGGGVSSLNMQLRK 121

Query: 123 ELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSS 182
           ELD++ASLVNC N PGL TRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKVIT+F S
Sbjct: 122 ELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCS 181

Query: 183 DRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCC 242
           +RIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA  Y  I YNE+IVDNCC
Sbjct: 182 ERIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGITYNEIIVDNCC 241

Query: 243 MQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGN 302
           MQLV+KPEQFDVMVTPNLYGNL+ANTAAGIAGGTG+MPGGNVGA+HAIFEQGASAGNVGN
Sbjct: 242 MQLVAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIFEQGASAGNVGN 301

Query: 303 EKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQD 362
           +K+VE+KKANPVALLLSSAMMLRHL+FP+FADRLE+AVK+VI E K RTKDLGG+CTTQ+
Sbjct: 302 DKMVEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTTQE 361

Query: 363 VVDAVIAFLD 373
           VVDAVIA L+
Sbjct: 362 VVDAVIAALE 368

BLAST of Lag0009377 vs. ExPASy Swiss-Prot
Match: P93032 (Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=IDH2 PE=1 SV=2)

HSP 1 Score: 596.3 bits (1536), Expect = 2.4e-169
Identity = 295/370 (79.73%), Postives = 333/370 (90.00%), Query Frame = 0

Query: 3   ARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVE 62
           +R++F +++ L S +  S        RSVTYM RPGDG PRPVTLIPGDG+GPLVTNAV+
Sbjct: 2   SRQSFSLLKNLRSIASGSKI----QTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNAVQ 61

Query: 63  QVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRK 122
           QVMEA+HAPVYFE F+VHGDMK +P  ++ESI +NKVCLKGGL+TP+GGGVSSLNV LRK
Sbjct: 62  QVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGGGVSSLNVNLRK 121

Query: 123 ELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSS 182
           ELDL+ASLVNCFN PGL +RHENVDIVVIRENTEGEY+GLEHEV+PGVVESLKVIT+F S
Sbjct: 122 ELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCS 181

Query: 183 DRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCC 242
           +RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA +YPSI YNE+IVDNCC
Sbjct: 182 ERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDNCC 241

Query: 243 MQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGN 302
           MQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVGA++A+FEQGASAGNVG 
Sbjct: 242 MQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVFEQGASAGNVGK 301

Query: 303 EKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQD 362
           +   E+K ANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVI E  CRT+DLGGN TTQ+
Sbjct: 302 DTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQE 361

Query: 363 VVDAVIAFLD 373
           VVDAVIA LD
Sbjct: 362 VVDAVIANLD 367

BLAST of Lag0009377 vs. ExPASy Swiss-Prot
Match: Q54B68 (Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial OS=Dictyostelium discoideum OX=44689 GN=idhB PE=3 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 2.0e-91
Identity = 179/332 (53.92%), Postives = 231/332 (69.58%), Query Frame = 0

Query: 43  RPVTLIPGDGIGPLVTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLK 102
           + VT+IPGDGIGP +T++V  V +A   P+ +E FD+ G  + + +E++ SI RNKV LK
Sbjct: 30  KTVTVIPGDGIGPEITSSVMGVFQAAKVPIEWEIFDISGG-QPISQELIASITRNKVALK 89

Query: 103 GGLRTPMGGGVSSLNVQLRKELDLYASLVNCFNFPGLPTRHEN--VDIVVIRENTEGEYS 162
           G L T +  G  S N++LRK LDLYA +V C   PG+  RH++  VD VVIRENT+GEYS
Sbjct: 90  GPLYTEILSGSQSRNMELRKALDLYAHVVPCKQIPGITARHDDVLVDFVVIRENTQGEYS 149

Query: 163 GLEHEVIPGVVESLKVITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLES 222
           GLE  + PGVV+SLK+IT+ +S+RIARYAFEYA  N RKKVTAVHKANI K  DGLFL +
Sbjct: 150 GLEQVLTPGVVQSLKIITKEASERIARYAFEYAKANGRKKVTAVHKANIQKQTDGLFLAT 209

Query: 223 CREVATQYPSIQYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMP 282
           C ++A +YP I++   I+DNCCMQLV  PEQ+DVMVTPNLYGN+V+N  A + GG GL  
Sbjct: 210 CTQIAKEYPEIKFENTIIDNCCMQLVKSPEQYDVMVTPNLYGNIVSNIGAALVGGPGLAG 269

Query: 283 GGNVGADHAIFEQGASAGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAV 342
           G NVG    IFE GA   +V  + I  K KANP  LLL+S MML+HL     A ++E+AV
Sbjct: 270 GANVGEGSIIFEMGAH--HVAAD-IAGKDKANPTGLLLASVMMLKHLGLNEHATKVENAV 329

Query: 343 KRVIMEDKCRTKDLGGNCTTQDVVDAVIAFLD 373
           K VI E    T D+GG  +T+    AVI +++
Sbjct: 330 KAVIKEGTL-TSDIGGKSSTKQFTGAVIDYIE 356

BLAST of Lag0009377 vs. ExPASy Swiss-Prot
Match: O13696 (Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=idh1 PE=1 SV=1)

HSP 1 Score: 325.1 bits (832), Expect = 1.0e-87
Identity = 173/336 (51.49%), Postives = 227/336 (67.56%), Query Frame = 0

Query: 40  GAPRPVTLIPGDGIGPLVTNAVEQVMEALHAPVYFERFDVHGDMK------QVPREVMES 99
           G    VTLIPGDGIG   +NAV ++ +  + P+ FE  DV G  K          E ++S
Sbjct: 18  GGKYTVTLIPGDGIGRETSNAVTEIFKTANVPIEFEEIDVTGMEKNNKSSGDALHEAIQS 77

Query: 100 IWRNKVCLKGGLRTPM-GGGVSSLNVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIR 159
           + RNKV LKG L TP   GG +S NV LRKELD+YASLV   N PG  TRH+NVD  +IR
Sbjct: 78  LKRNKVGLKGILFTPFEKGGHTSFNVALRKELDIYASLVLIKNIPGFKTRHDNVDFAIIR 137

Query: 160 ENTEGEYSGLEHEVIPGVVESLKVITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKL 219
           ENTEGEYSGLEH+ +PGVVESLK+IT++ S RIA++AF++A  N RK VT +HKANIMKL
Sbjct: 138 ENTEGEYSGLEHQSVPGVVESLKIITEYKSKRIAQFAFDFALQNGRKSVTCIHKANIMKL 197

Query: 220 ADGLFLESCREVATQYPSIQYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGI 279
           ADGLF  +  +VA  Y +I   ++IVDN  MQ VS+P+QFDV+V PNLYG++++N  + +
Sbjct: 198 ADGLFRRTFYDVANGYDAITPKDLIVDNASMQAVSRPQQFDVLVMPNLYGSILSNIGSAL 257

Query: 280 AGGTGLMPGGNVGADHAIFEQGASAGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSF 339
            GG G++PG N G D+A+FE G    +VG   I  + +ANP A +LS+ +MLRHL    +
Sbjct: 258 VGGPGVIPGANFGRDYALFEPGCR--HVG-LSITGRGEANPTAAILSACLMLRHLGLKDY 317

Query: 340 ADRLESAVKRVIMEDKCRTKDLGGNCTTQDVVDAVI 369
           AD + +A   VI E K  TKDLGG+ +T D   A++
Sbjct: 318 ADLINAATYSVIEEGKTLTKDLGGSASTGDFTHAIL 350

BLAST of Lag0009377 vs. ExPASy TrEMBL
Match: A0A6J1I0V5 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111469830 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 7.0e-196
Identity = 351/376 (93.35%), Postives = 362/376 (96.28%), Query Frame = 0

Query: 1   MAARRAFPIIRRLA----SRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MAAR AFPII RLA    SR +LSPF LFSDARSVTYM RPGDGAPR VTLIPGDGIGPL
Sbjct: 1   MAARSAFPIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEALHAPVYFER+DVHG+MKQVPREVMESI++NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEALHAPVYFERYDVHGNMKQVPREVMESIFKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGL TRHENVDI VIRENTEGEYSGLEHEVIPGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYSGLEHEVIPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYP IQYNEV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVGADHAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVIMEDKCRTKDLGG
Sbjct: 301 AGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQDVVDAVIAFLD
Sbjct: 361 NCTTQDVVDAVIAFLD 376

BLAST of Lag0009377 vs. ExPASy TrEMBL
Match: A0A6J1G859 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111451756 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 5.0e-194
Identity = 348/376 (92.55%), Postives = 361/376 (96.01%), Query Frame = 0

Query: 1   MAARRAFPIIRRLA----SRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MAAR AF II RLA    SR +LSPF LFSDARSVTYM RPGDGAPR VTLIPGDGIGPL
Sbjct: 1   MAARSAFHIICRLASPYRSRFQLSPFNLFSDARSVTYMPRPGDGAPRAVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEALHAPVYFER+DVHG+MK+VPREVMESI++NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEALHAPVYFERYDVHGNMKRVPREVMESIFKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGL TRHENVDI VIRENTEGEY+GLEHEVIPGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLLTRHENVDIAVIRENTEGEYAGLEHEVIPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYP IQYNEV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPIIQYNEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAG+AGGTGLMPGGNVGADHAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGLAGGTGLMPGGNVGADHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVIMEDKCRTKDLGG
Sbjct: 301 AGNVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVIMEDKCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQDVVDAVIAFLD
Sbjct: 361 NCTTQDVVDAVIAFLD 376

BLAST of Lag0009377 vs. ExPASy TrEMBL
Match: A0A6J1FV85 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111448406 PE=3 SV=1)

HSP 1 Score: 669.5 bits (1726), Expect = 8.3e-189
Identity = 335/376 (89.10%), Postives = 357/376 (94.95%), Query Frame = 0

Query: 1   MAARRAFPIIRRL----ASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MA RRAFPIIR L     SRSRLSPF LF D+RSVTYM RPG+G  RPVTLIPGDGIGPL
Sbjct: 1   MAGRRAFPIIRGLISLSRSRSRLSPFNLFFDSRSVTYMPRPGEGDLRPVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEA+HAPVYFER++VHGDMK+VPREVMESI +NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEAMHAPVYFERYEVHGDMKEVPREVMESILKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVLPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKL+DGLFLESCREVAT+YPSI+Y EV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLSDGLFLESCREVATEYPSIEYKEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVG +HAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGFEHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVI+E  CRTKDLGG
Sbjct: 301 AGNVGNEKIVEEKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVILEGNCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQ +VDAVIA+++
Sbjct: 361 NCTTQRLVDAVIAYVE 376

BLAST of Lag0009377 vs. ExPASy TrEMBL
Match: A0A6J1J614 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111483775 PE=3 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.8e-188
Identity = 334/376 (88.83%), Postives = 357/376 (94.95%), Query Frame = 0

Query: 1   MAARRAFPIIRRL----ASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPL 60
           MA RRAFPIIR L     SRSRLSPF LF D+RSVTYM RPG+G  RPVTLIPGDGIGPL
Sbjct: 1   MAGRRAFPIIRGLISPSRSRSRLSPFNLFFDSRSVTYMPRPGEGDLRPVTLIPGDGIGPL 60

Query: 61  VTNAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSL 120
           VTNAVEQVMEA+HAPVYFER++VHGDMK+VPREVMESI +NKVCLKGGL TPMGGGVSSL
Sbjct: 61  VTNAVEQVMEAMHAPVYFERYEVHGDMKEVPREVMESILKNKVCLKGGLMTPMGGGVSSL 120

Query: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKV 180
           NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKV
Sbjct: 121 NVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVLPGVVESLKV 180

Query: 181 ITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEV 240
           IT+FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKL+DGLFLESCREVAT+YPSI+Y EV
Sbjct: 181 ITKFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLSDGLFLESCREVATEYPSIEYKEV 240

Query: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGAS 300
           IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVG +HAIFEQGAS
Sbjct: 241 IVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGFEHAIFEQGAS 300

Query: 301 AGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGG 360
           AGNVGN+KIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVI+E  CRTKDLGG
Sbjct: 301 AGNVGNDKIVEEKKANPVALLLSSAMMLRHLQFPSFADRLENAVKRVILEGNCRTKDLGG 360

Query: 361 NCTTQDVVDAVIAFLD 373
           NCTTQ +VDAVIA+++
Sbjct: 361 NCTTQRLVDAVIAYVE 376

BLAST of Lag0009377 vs. ExPASy TrEMBL
Match: A0A6J1CFI7 (isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Momordica charantia OX=3673 GN=LOC111011213 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.4e-188
Identity = 337/374 (90.11%), Postives = 353/374 (94.39%), Query Frame = 0

Query: 1   MAARRAFPIIRRLAS--RSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVT 60
           MAARRAFPIIR L S  RSR     L  D RSVTYM RPG+G+PR VTLIPGDGIGPLVT
Sbjct: 1   MAARRAFPIIRHLTSPCRSRSRILPLRFDTRSVTYMPRPGEGSPRQVTLIPGDGIGPLVT 60

Query: 61  NAVEQVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNV 120
           NAVEQVMEA+HAPVYFER++VHGDMKQVPREVMESI +NKVCLKGGL TPMGGGVSSLNV
Sbjct: 61  NAVEQVMEAMHAPVYFERYEVHGDMKQVPREVMESILKNKVCLKGGLITPMGGGVSSLNV 120

Query: 121 QLRKELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVIT 180
           QLRKELDLYASLVNCFNFPG PTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVIT
Sbjct: 121 QLRKELDLYASLVNCFNFPGFPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVIT 180

Query: 181 QFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIV 240
           +FSS+RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA QYPSIQYNE+IV
Sbjct: 181 KFSSERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAAQYPSIQYNEIIV 240

Query: 241 DNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAG 300
           DNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVG+DHAIFEQGASAG
Sbjct: 241 DNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGSDHAIFEQGASAG 300

Query: 301 NVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNC 360
           NVGNEKIVE+KKANPVALLLSSAMMLRHLQFPSFADRLE+AVK VI+E KCRTKDLGG+C
Sbjct: 301 NVGNEKIVEQKKANPVALLLSSAMMLRHLQFPSFADRLENAVKAVILEAKCRTKDLGGDC 360

Query: 361 TTQDVVDAVIAFLD 373
           TTQDVVDAVI+ LD
Sbjct: 361 TTQDVVDAVISLLD 374

BLAST of Lag0009377 vs. TAIR 10
Match: AT4G35260.1 (isocitrate dehydrogenase 1 )

HSP 1 Score: 615.9 bits (1587), Expect = 2.1e-176
Identity = 306/370 (82.70%), Postives = 338/370 (91.35%), Query Frame = 0

Query: 3   ARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVE 62
           +RR+  +++ LA  +  S        RSVTYM RPGDGAPR VTLIPGDGIGPLVTNAVE
Sbjct: 2   SRRSLTLLKNLARNANGSGI----QTRSVTYMPRPGDGAPRAVTLIPGDGIGPLVTNAVE 61

Query: 63  QVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRK 122
           QVMEA+HAP++FE++DVHG+M +VP EVMESI +NKVCLKGGL+TP+GGGVSSLNVQLRK
Sbjct: 62  QVMEAMHAPIFFEKYDVHGEMSRVPPEVMESIRKNKVCLKGGLKTPVGGGVSSLNVQLRK 121

Query: 123 ELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSS 182
           ELDL+ASLVNCFN PGLPTRHENVDIVVIRENTEGEY+GLEHEV+PGVVESLKVIT+F S
Sbjct: 122 ELDLFASLVNCFNLPGLPTRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCS 181

Query: 183 DRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCC 242
           +RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA +YPSI YNE+IVDNCC
Sbjct: 182 ERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKKYPSITYNEIIVDNCC 241

Query: 243 MQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGN 302
           MQLV+KPEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVGADHA+FEQGASAGNVG 
Sbjct: 242 MQLVAKPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGADHAVFEQGASAGNVGK 301

Query: 303 EKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQD 362
           +KIV + KANPVALLLSSAMMLRHLQFPSFADRLE+AVK+VI E KCRTKDLGG  TTQ+
Sbjct: 302 DKIVLENKANPVALLLSSAMMLRHLQFPSFADRLETAVKKVIAEGKCRTKDLGGTSTTQE 361

Query: 363 VVDAVIAFLD 373
           VVDAVIA LD
Sbjct: 362 VVDAVIAKLD 367

BLAST of Lag0009377 vs. TAIR 10
Match: AT4G35650.1 (isocitrate dehydrogenase III )

HSP 1 Score: 602.8 bits (1553), Expect = 1.8e-172
Identity = 303/370 (81.89%), Postives = 337/370 (91.08%), Query Frame = 0

Query: 3   ARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVE 62
           ARR+  I  RL +    SPFT  S  RS+TYM RPGDGAPR VTLIPGDGIGPLVT AVE
Sbjct: 2   ARRSVSIFNRLLANPP-SPFTSLS--RSITYMPRPGDGAPRTVTLIPGDGIGPLVTGAVE 61

Query: 63  QVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRK 122
           QVMEA+HAPV+FER++V G+M++VP EV+ES+ RNKVCLKGGL TP+GGGVSSLN+QLRK
Sbjct: 62  QVMEAMHAPVHFERYEVLGNMRKVPEEVIESVKRNKVCLKGGLATPVGGGVSSLNMQLRK 121

Query: 123 ELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSS 182
           ELD++ASLVNC N PGL TRHENVDIVVIRENTEGEYSGLEHEV+PGVVESLKVIT+F S
Sbjct: 122 ELDIFASLVNCINVPGLVTRHENVDIVVIRENTEGEYSGLEHEVVPGVVESLKVITKFCS 181

Query: 183 DRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCC 242
           +RIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVA  Y  I YNE+IVDNCC
Sbjct: 182 ERIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVAKHYSGITYNEIIVDNCC 241

Query: 243 MQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGN 302
           MQLV+KPEQFDVMVTPNLYGNL+ANTAAGIAGGTG+MPGGNVGA+HAIFEQGASAGNVGN
Sbjct: 242 MQLVAKPEQFDVMVTPNLYGNLIANTAAGIAGGTGVMPGGNVGAEHAIFEQGASAGNVGN 301

Query: 303 EKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQD 362
           +K+VE+KKANPVALLLSSAMMLRHL+FP+FADRLE+AVK+VI E K RTKDLGG+CTTQ+
Sbjct: 302 DKMVEQKKANPVALLLSSAMMLRHLRFPTFADRLETAVKQVIKEGKYRTKDLGGDCTTQE 361

Query: 363 VVDAVIAFLD 373
           VVDAVIA L+
Sbjct: 362 VVDAVIAALE 368

BLAST of Lag0009377 vs. TAIR 10
Match: AT2G17130.1 (isocitrate dehydrogenase subunit 2 )

HSP 1 Score: 596.3 bits (1536), Expect = 1.7e-170
Identity = 295/370 (79.73%), Postives = 333/370 (90.00%), Query Frame = 0

Query: 3   ARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVE 62
           +R++F +++ L S +  S        RSVTYM RPGDG PRPVTLIPGDG+GPLVTNAV+
Sbjct: 2   SRQSFSLLKNLRSIASGSKI----QTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNAVQ 61

Query: 63  QVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRK 122
           QVMEA+HAPVYFE F+VHGDMK +P  ++ESI +NKVCLKGGL+TP+GGGVSSLNV LRK
Sbjct: 62  QVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGGGVSSLNVNLRK 121

Query: 123 ELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSS 182
           ELDL+ASLVNCFN PGL +RHENVDIVVIRENTEGEY+GLEHEV+PGVVESLKVIT+F S
Sbjct: 122 ELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLKVITKFCS 181

Query: 183 DRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCC 242
           +RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA +YPSI YNE+IVDNCC
Sbjct: 182 ERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDNCC 241

Query: 243 MQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGN 302
           MQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVGA++A+FEQGASAGNVG 
Sbjct: 242 MQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVFEQGASAGNVGK 301

Query: 303 EKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQD 362
           +   E+K ANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVI E  CRT+DLGGN TTQ+
Sbjct: 302 DTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQE 361

Query: 363 VVDAVIAFLD 373
           VVDAVIA LD
Sbjct: 362 VVDAVIANLD 367

BLAST of Lag0009377 vs. TAIR 10
Match: AT2G17130.2 (isocitrate dehydrogenase subunit 2 )

HSP 1 Score: 585.1 bits (1507), Expect = 4.0e-167
Identity = 292/370 (78.92%), Postives = 329/370 (88.92%), Query Frame = 0

Query: 3   ARRAFPIIRRLASRSRLSPFTLFSDARSVTYMHRPGDGAPRPVTLIPGDGIGPLVTNAVE 62
           +R++F +++ L S +  S        RSVTYM RPGDG PRPVTLIPGDG+GPLVTNAV+
Sbjct: 2   SRQSFSLLKNLRSIASGSKI----QTRSVTYMPRPGDGKPRPVTLIPGDGVGPLVTNAVQ 61

Query: 63  QVMEALHAPVYFERFDVHGDMKQVPREVMESIWRNKVCLKGGLRTPMGGGVSSLNVQLRK 122
           QVMEA+HAPVYFE F+VHGDMK +P  ++ESI +NKVCLKGGL+TP+GGGVSSLNV LRK
Sbjct: 62  QVMEAMHAPVYFEPFEVHGDMKSLPEGLLESIKKNKVCLKGGLKTPVGGGVSSLNVNLRK 121

Query: 123 ELDLYASLVNCFNFPGLPTRHENVDIVVIRENTEGEYSGLEHEVIPGVVESLKVITQFSS 182
           ELDL+ASLVNCFN PGL +RHENVDIVVIRENTEGEY+GLEHEV+PGVVESLK    F S
Sbjct: 122 ELDLFASLVNCFNLPGLASRHENVDIVVIRENTEGEYAGLEHEVVPGVVESLK----FCS 181

Query: 183 DRIARYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCREVATQYPSIQYNEVIVDNCC 242
           +RIA+YAFEYAYLNNRKKVTAVHKANIMKLADGLFLESC+EVA +YPSI YNE+IVDNCC
Sbjct: 182 ERIAKYAFEYAYLNNRKKVTAVHKANIMKLADGLFLESCQEVAKKYPSIAYNEIIVDNCC 241

Query: 243 MQLVSKPEQFDVMVTPNLYGNLVANTAAGIAGGTGLMPGGNVGADHAIFEQGASAGNVGN 302
           MQLV++PEQFDVMVTPNLYGNLVANTAAGIAGGTG+MPGGNVGA++A+FEQGASAGNVG 
Sbjct: 242 MQLVARPEQFDVMVTPNLYGNLVANTAAGIAGGTGVMPGGNVGAEYAVFEQGASAGNVGK 301

Query: 303 EKIVEKKKANPVALLLSSAMMLRHLQFPSFADRLESAVKRVIMEDKCRTKDLGGNCTTQD 362
           +   E+K ANPVALLLSSAMMLRHLQFPSFADRLE+AVKRVI E  CRT+DLGGN TTQ+
Sbjct: 302 DTTEEQKNANPVALLLSSAMMLRHLQFPSFADRLETAVKRVIAEGNCRTEDLGGNSTTQE 361

Query: 363 VVDAVIAFLD 373
           VVDAVIA LD
Sbjct: 362 VVDAVIANLD 363

BLAST of Lag0009377 vs. TAIR 10
Match: AT5G03290.1 (isocitrate dehydrogenase V )

HSP 1 Score: 319.7 bits (818), Expect = 3.1e-87
Identity = 167/333 (50.15%), Postives = 223/333 (66.97%), Query Frame = 0

Query: 42  PRPVTLIPGDGIGPLVTNAVEQVMEALHAPVYFERFDVHGDMKQVPR-------EVMESI 101
           P   TL PGDGIGP +  +V++V      P+ +E   V  ++   PR       E +ES+
Sbjct: 43  PITATLFPGDGIGPEIAESVKKVFTTAGVPIEWEEHYVGTEID--PRTQSFLTWESLESV 102

Query: 102 WRNKVCLKGGLRTPMGGGVSSLNVQLRKELDLYASLVNCFNFPGLPTRHENVDIVVIREN 161
            RNKV LKG + TP+G G  SLN+ LRKEL+LYA++  C++ PG  TR+++VD++ IREN
Sbjct: 103 RRNKVGLKGPMATPIGKGHRSLNLTLRKELNLYANVRPCYSLPGYKTRYDDVDLITIREN 162

Query: 162 TEGEYSGLEHEVIPGVVESLKVITQFSSDRIARYAFEYAYLNNRKKVTAVHKANIMKLAD 221
           TEGEYSGLEH+V+ GVVESLK+IT+ +S R+A YAF YA  + R++V+A+HKANIM+  D
Sbjct: 163 TEGEYSGLEHQVVRGVVESLKIITRQASLRVAEYAFLYAKTHGRERVSAIHKANIMQKTD 222

Query: 222 GLFLESCREVATQYPSIQYNEVIVDNCCMQLVSKPEQFDVMVTPNLYGNLVANTAAGIAG 281
           GLFL+ CREVA +YP I Y EV++DNCCM LV  P  FDV+V PNLYG+++++  AG+ G
Sbjct: 223 GLFLKCCREVAEKYPEITYEEVVIDNCCMMLVKNPALFDVLVMPNLYGDIISDLCAGLVG 282

Query: 282 GTGLMPGGNVGADHAIFEQGASAGNVGNEKIVEKKKANPVALLLSSAMMLRHLQFPSFAD 341
           G GL P  N+G D       A A +     I  K  ANP ALLLS  MMLRHL+F   A+
Sbjct: 283 GLGLTPSCNIGEDGVAL---AEAVHGSAPDIAGKNLANPTALLLSGVMMLRHLKFNEQAE 342

Query: 342 RLESAVKRVIMEDKCRTKDLGGNCTTQDVVDAV 368
           ++ SA+   I E K RT DLGG+ TT +   A+
Sbjct: 343 QIHSAIINTIAEGKYRTADLGGSSTTTEFTKAI 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022971037.11.4e-19593.35isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbi... [more]
XP_023534325.13.2e-19593.09isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbi... [more]
KAG6604970.14.2e-19592.82Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cuc... [more]
KAG7035004.15.5e-19592.82Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial, partial [Cuc... [more]
XP_022948062.11.0e-19392.55isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like [Cucurbi... [more]
Match NameE-valueIdentityDescription
Q8LFC02.9e-17582.70Isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial OS=Arabidopsi... [more]
O817962.6e-17181.89Isocitrate dehydrogenase [NAD] regulatory subunit 3, mitochondrial OS=Arabidopsi... [more]
P930322.4e-16979.73Isocitrate dehydrogenase [NAD] regulatory subunit 2, mitochondrial OS=Arabidopsi... [more]
Q54B682.0e-9153.92Isocitrate dehydrogenase [NAD] regulatory subunit B, mitochondrial OS=Dictyostel... [more]
O136961.0e-8751.49Isocitrate dehydrogenase [NAD] subunit 1, mitochondrial OS=Schizosaccharomyces p... [more]
Match NameE-valueIdentityDescription
A0A6J1I0V57.0e-19693.35isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1G8595.0e-19492.55isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1FV858.3e-18989.10isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1J6141.8e-18888.83isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Cucur... [more]
A0A6J1CFI72.4e-18890.11isocitrate dehydrogenase [NAD] regulatory subunit 1, mitochondrial-like OS=Momor... [more]
Match NameE-valueIdentityDescription
AT4G35260.12.1e-17682.70isocitrate dehydrogenase 1 [more]
AT4G35650.11.8e-17281.89isocitrate dehydrogenase III [more]
AT2G17130.11.7e-17079.73isocitrate dehydrogenase subunit 2 [more]
AT2G17130.24.0e-16778.92isocitrate dehydrogenase subunit 2 [more]
AT5G03290.13.1e-8750.15isocitrate dehydrogenase V [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024084Isopropylmalate dehydrogenase-like domainSMARTSM01329Iso_dh_2coord: 44..367
e-value: 1.4E-141
score: 486.3
IPR024084Isopropylmalate dehydrogenase-like domainPFAMPF00180Iso_dhcoord: 45..367
e-value: 4.4E-80
score: 269.5
NoneNo IPR availableGENE3D3.40.718.10Isopropylmalate Dehydrogenasecoord: 28..370
e-value: 6.5E-126
score: 421.9
NoneNo IPR availablePANTHERPTHR11835DECARBOXYLATING DEHYDROGENASES-ISOCITRATE, ISOPROPYLMALATE, TARTRATEcoord: 4..370
NoneNo IPR availablePANTHERPTHR11835:SF56NAD-DEPENDENT ISOCITRATE DEHYDROGENASE C,1coord: 4..370
NoneNo IPR availableSUPERFAMILY53659Isocitrate/Isopropylmalate dehydrogenase-likecoord: 43..371
IPR004434Isocitrate dehydrogenase NAD-dependentTIGRFAMTIGR00175TIGR00175coord: 42..369
e-value: 9.0E-156
score: 516.1

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009377.1Lag0009377.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006102 isocitrate metabolic process
biological_process GO:0006099 tricarboxylic acid cycle
cellular_component GO:0005739 mitochondrion
molecular_function GO:0004449 isocitrate dehydrogenase (NAD+) activity
molecular_function GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor