Lag0009061 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009061
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionalpha/beta-Hydrolases superfamily protein
Locationchr9: 34812511 .. 34813434 (-)
RNA-Seq ExpressionLag0009061
SyntenyLag0009061
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCCGCCGACGCCGCCGGAGAAGAAGTAGTTTTCGATTTCCATCCCATCTTCCGCGTCTTCAAAAGCGGCCGCGTCGAGAGGTTTTTTCCCGCCGACCGCGTCCCGCCGTCCACCGATCCACTCACCGGCGTCCAATCCAAAGACGTCCTCATAGATCCACACACCCAACTCTCCGTCCGGATCTACCTCCCGCAAGTTCCAGACTCGACCAACAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCCTTCGCCATCGGCTCCGTCGCGTCCCCAATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAGGCCAATGCCGTCGCCCTCTCGGTCGAATACCGCCTCGCGCCGGAGCATCCTCTCCCGGCGGCGTACGACGACGCCTGGGCGGCGGTGGAGTGGGCGGCGTCGCACTCCAACCGCGACGGCGCCGAGACTTGGCTGAACGAACACGCGGATTTCGACCGCGTTTTCCTCGTCGGGGACAGCGCTGGGGCGAACATTTCGTACAACGTGGTGGGCCGGGCCAGCGAGAGTGATAATGGGCCGAAGGGATTGAACATCATCGGGCTGGGCCTGGTCCATCCGTTTTTCATGATCGACAAGCCCGATAAGTTGATCGAGTACATCTTCCCGACGTCGGCGGGTCTGGAGGATCCGAAGATGAACCCGTGGGTCAACCCGGGACTAGGGAGGTTACGGTGCAACCGGGTGCTGATACTGGCGGCGGAACGCGATTTTCTGAAGGACAGGGCGCGTGGGTTTCACGCGGCGTTGAGGGAGAGTGGGTGGGAAGGGTCGGTGGAGATGGTGGAGACCGAAGGAGAGGAGCACGTGTTTCATTTGTTTAACCCGGACTGTGAAAAGGCTGTTGCGTTGGTCAAAAAAGTGGCTTCCTTCATCCAATCAGCGTAA

mRNA sequence

ATGGATTCCGCCGACGCCGCCGGAGAAGAAGTAGTTTTCGATTTCCATCCCATCTTCCGCGTCTTCAAAAGCGGCCGCGTCGAGAGGTTTTTTCCCGCCGACCGCGTCCCGCCGTCCACCGATCCACTCACCGGCGTCCAATCCAAAGACGTCCTCATAGATCCACACACCCAACTCTCCGTCCGGATCTACCTCCCGCAAGTTCCAGACTCGACCAACAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCCTTCGCCATCGGCTCCGTCGCGTCCCCAATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAGGCCAATGCCGTCGCCCTCTCGGTCGAATACCGCCTCGCGCCGGAGCATCCTCTCCCGGCGGCGTACGACGACGCCTGGGCGGCGGTGGAGTGGGCGGCGTCGCACTCCAACCGCGACGGCGCCGAGACTTGGCTGAACGAACACGCGGATTTCGACCGCGTTTTCCTCGTCGGGGACAGCGCTGGGGCGAACATTTCGTACAACGTGGTGGGCCGGGCCAGCGAGAGTGATAATGGGCCGAAGGGATTGAACATCATCGGGCTGGGCCTGGTCCATCCGTTTTTCATGATCGACAAGCCCGATAAGTTGATCGAGTACATCTTCCCGACGTCGGCGGGTCTGGAGGATCCGAAGATGAACCCGTGGGTCAACCCGGGACTAGGGAGGTTACGGTGCAACCGGGTGCTGATACTGGCGGCGGAACGCGATTTTCTGAAGGACAGGGCGCGTGGGTTTCACGCGGCGTTGAGGGAGAGTGGGTGGGAAGGGTCGGTGGAGATGGTGGAGACCGAAGGAGAGGAGCACGTGTTTCATTTGTTTAACCCGGACTGTGAAAAGGCTGTTGCGTTGGTCAAAAAAGTGGCTTCCTTCATCCAATCAGCGTAA

Coding sequence (CDS)

ATGGATTCCGCCGACGCCGCCGGAGAAGAAGTAGTTTTCGATTTCCATCCCATCTTCCGCGTCTTCAAAAGCGGCCGCGTCGAGAGGTTTTTTCCCGCCGACCGCGTCCCGCCGTCCACCGATCCACTCACCGGCGTCCAATCCAAAGACGTCCTCATAGATCCACACACCCAACTCTCCGTCCGGATCTACCTCCCGCAAGTTCCAGACTCGACCAACAAGCTCCCGCTCCTAATTTACATCCACGGCGGCGCCTTCGCCATCGGCTCCGTCGCGTCCCCAATCTACCACCGCCACCTGATCTCCCTCGCCGCCGAGGCCAATGCCGTCGCCCTCTCGGTCGAATACCGCCTCGCGCCGGAGCATCCTCTCCCGGCGGCGTACGACGACGCCTGGGCGGCGGTGGAGTGGGCGGCGTCGCACTCCAACCGCGACGGCGCCGAGACTTGGCTGAACGAACACGCGGATTTCGACCGCGTTTTCCTCGTCGGGGACAGCGCTGGGGCGAACATTTCGTACAACGTGGTGGGCCGGGCCAGCGAGAGTGATAATGGGCCGAAGGGATTGAACATCATCGGGCTGGGCCTGGTCCATCCGTTTTTCATGATCGACAAGCCCGATAAGTTGATCGAGTACATCTTCCCGACGTCGGCGGGTCTGGAGGATCCGAAGATGAACCCGTGGGTCAACCCGGGACTAGGGAGGTTACGGTGCAACCGGGTGCTGATACTGGCGGCGGAACGCGATTTTCTGAAGGACAGGGCGCGTGGGTTTCACGCGGCGTTGAGGGAGAGTGGGTGGGAAGGGTCGGTGGAGATGGTGGAGACCGAAGGAGAGGAGCACGTGTTTCATTTGTTTAACCCGGACTGTGAAAAGGCTGTTGCGTTGGTCAAAAAAGTGGCTTCCTTCATCCAATCAGCGTAA

Protein sequence

MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKVASFIQSA
Homology
BLAST of Lag0009061 vs. NCBI nr
Match: KAG6605131.1 (putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.3 bits (1396), Expect = 2.6e-150
Identity = 267/306 (87.25%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS   AGEEVVF F  +FRVFKSGRVER  PAD VPPSTD  TGVQSKDVLIDPHT+LS
Sbjct: 1   MDS--TAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP+V  S  KLPLLIYIHGGAFAIGSVAS IYH HLISLAAEANAVA+SVEYRLAP
Sbjct: 61  VRIYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           E+PLPAAYDDAW AVEW ASHSNRDGAE WLNEHADFDRVF+VGDSAGANISYNVVGRAS
Sbjct: 121 ENPLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRAS 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
           E+DNGP+GL IIGLGLVHPFFMIDKPDKLIEYIFPTS GLEDP+MNPW NPGLGRLRCNR
Sbjct: 181 ENDNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRLRCNR 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           VLI+AAE DFL DRARGFHAALRESGW+GSVEMVE EGE+HVFHLFNPDCEKAVALVK V
Sbjct: 241 VLIIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKTV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 304

BLAST of Lag0009061 vs. NCBI nr
Match: XP_023532126.1 (probable carboxylesterase 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 542.0 bits (1395), Expect = 3.4e-150
Identity = 266/306 (86.93%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS   AGEEVVF F  +FRVFKSGRVER  PAD VPPSTD  TGVQSKDVLIDPHT+LS
Sbjct: 1   MDS--TAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP+V  S  KLPLLIYIHGGAF+IGSVAS IYH HLISLAAEANAVA+SVEYRLAP
Sbjct: 61  VRIYLPKVSTSAKKLPLLIYIHGGAFSIGSVASSIYHSHLISLAAEANAVAVSVEYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           E+PLPAAYDDAW AVEW ASHSNRDGAE WLNEHADFDRVF+VGDSAGANISYNVVGRAS
Sbjct: 121 ENPLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRAS 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
           E+DNGP+GL IIGLGLVHPFFMIDKPDKLIEYIFPTS GLEDP+MNPW NPGLGR RCNR
Sbjct: 181 ENDNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNR 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           VLI+AAE DFL DRARGFHAALRESGW+GSVEMVE EGE+HVFHLFNPDCEKAVALVKKV
Sbjct: 241 VLIIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 304

BLAST of Lag0009061 vs. NCBI nr
Match: KAG7035128.1 (putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 541.2 bits (1393), Expect = 5.8e-150
Identity = 266/306 (86.93%), Postives = 279/306 (91.18%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS   AGEEVVF F  +FRVFKSGRVER  PAD VPPSTD  TGV+SKDVLIDPHT+LS
Sbjct: 1   MDS--TAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVESKDVLIDPHTKLS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP+V  S  KLPLLIYIHGGAFAIGSVAS IYH HLISLAAEANAVA+SVEYRLAP
Sbjct: 61  VRIYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           E+PLPAAYDDAW AVEW ASHSNRDGAE WLNEHADFDRVF+VGDSAGANISYNVVGRAS
Sbjct: 121 ENPLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRAS 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
           E+DNGP+GL IIGLGLVHPFFMIDKPDKLIEYIFPTS GLEDP+MNPW NPGLGRLRCNR
Sbjct: 181 ENDNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRLRCNR 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           VLI+AAE DFL DRARGFHAALRESGW+GSVEMVE EGE+HVFHLFNPDCEKAVALVK V
Sbjct: 241 VLIIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKTV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 304

BLAST of Lag0009061 vs. NCBI nr
Match: XP_023007510.1 (probable carboxylesterase 2 [Cucurbita maxima])

HSP 1 Score: 538.9 bits (1387), Expect = 2.9e-149
Identity = 265/306 (86.60%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS   AGEEVVF F  +FRVFKSGRVER  PAD VPPSTD  TGVQSKDVLIDPHT+LS
Sbjct: 1   MDS--TAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP+V  S  KLPLLIYIHGGAF IGSVAS IYH HLISLAAEANAVA+SVEYRLAP
Sbjct: 61  VRIYLPKVSTSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           E+PLPAAYDDAW AVEW ASHSNRDGAE WLNEHADFD VF+VGDSAGANISYNVVGRAS
Sbjct: 121 ENPLPAAYDDAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRAS 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
           E+DNGP+GLNIIGLGLVHPFFMIDKPDKLIEYIFPTS GLEDP+MNPW NPGL R RCNR
Sbjct: 181 ENDNGPEGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNR 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           VLI+AAE DFL DRARGFHAALRESGW+GSVEMVE EGE+HVFHLFNPDCEKAVALVKKV
Sbjct: 241 VLIIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 304

BLAST of Lag0009061 vs. NCBI nr
Match: XP_022948164.1 (probable carboxylesterase 2 [Cucurbita moschata])

HSP 1 Score: 538.1 bits (1385), Expect = 4.9e-149
Identity = 265/306 (86.60%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS   AGEEVVF F  +FRVFKSGRVER  PAD VPPSTD  TGVQSKDVLIDPHT+LS
Sbjct: 1   MDS--TAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP+V  S  KLPLLIYIHGGAFAIGSVAS IYH HLISLAAEANAVA+SVEYRLAP
Sbjct: 61  VRIYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           E+PLPAAYDDAW AVEW ASHSNRDGAE WLNEHADFDRVF+VGDSAGANISYNVVGRAS
Sbjct: 121 ENPLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRAS 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
           E+DNGP+GL IIGLGLVHPFFMIDKPDKLIEYIFPTS GLEDP+MNPW NPGLGR RCNR
Sbjct: 181 ENDNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNR 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           VLI+AAE DFL DRARGFHAALRESGW+GSVEMVE EGE+HVFHLFNPDC KAVALVK V
Sbjct: 241 VLIIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCGKAVALVKTV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 304

BLAST of Lag0009061 vs. ExPASy Swiss-Prot
Match: Q9SMN0 (Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.0e-68
Identity = 139/319 (43.57%), Postives = 197/319 (61.76%), Query Frame = 0

Query: 10  EVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQ-- 69
           E+  D  P+ +++KSGR+ER      VPPS++P  GV SKDV+      LSVRIYLP+  
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 70  VPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLPAA 129
             ++ +KLPLL+Y HGG F I +  SP YH  L +  + +N VA+SV+YR APEHP+   
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 130 YDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNGPK 189
           +DD+W A++W  +H    G E WLN+HADF RVFL GDSAGANI +++  RA++    P 
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSP- 183

Query: 190 GLN---IIGLGLVHPFFM----IDKPDKLIEYI-----------FPTSA-GLEDPKMNPW 249
           GLN   I G+ L+HP+F     ID+ D   E +            P S  G +DP +N  
Sbjct: 184 GLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVV 243

Query: 250 VNPG--LGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLF 306
            +    L  L C +VL++ AE+D L  +  G+ A L +SGW+G VE+VE+EGE+HVFHL 
Sbjct: 244 QSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLL 303

BLAST of Lag0009061 vs. ExPASy Swiss-Prot
Match: Q9SX78 (Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.6e-68
Identity = 133/316 (42.09%), Postives = 198/316 (62.66%), Query Frame = 0

Query: 5   DAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIY 64
           ++  ++V  +  P   V   G VER    +  PP  DP+TGV SKD++I+P T LS RIY
Sbjct: 2   ESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIY 61

Query: 65  LPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPL 124
            P       K+PL++Y HGGAF I S + P YH  L  +  +AN +A+SV YRLAPEHPL
Sbjct: 62  RPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPL 121

Query: 125 PAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDN 184
           P AY+D+W A++   + +     E W+N++AD D +FLVGDSAGANIS+++  RA +SD 
Sbjct: 122 PTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD- 181

Query: 185 GPKGLNIIGLGLVHPFFMIDKP--------------DKLIEYIFPTSAGLEDPKMNPWV- 244
             + L I G+G++HP+F   +P              D   E++ P+  G +DP +NP+  
Sbjct: 182 --QTLKIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFAD 241

Query: 245 -NPGLGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNP 304
            +P LG L C RV+I  AE+D L +R + ++  L +S W+G VE++ET+ ++HVFH+F P
Sbjct: 242 GSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEP 301

BLAST of Lag0009061 vs. ExPASy Swiss-Prot
Match: Q9LMA7 (Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 8.3e-67
Identity = 133/312 (42.63%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 10  EVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQ-- 69
           E+ FD+ P FR+FK+G +ER  P   VPPS +P  GV SKD +  P   LS+RIYLPQ  
Sbjct: 4   EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNS 63

Query: 70  -VPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLPA 129
                  K+PLL+Y HGG F + +  SPIYH  L S  +  + +A+SVEYR APEHP+P 
Sbjct: 64  VYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPT 123

Query: 130 AYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNGP 189
            Y+D+W A++W  +H  R G E WLN+HADF +VFL GDSAGANI++++  R  +    P
Sbjct: 124 LYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPP 183

Query: 190 KGLNIIGLGLVHPFFM------------IDKPDKLIEYIFPTSA-GLEDPKMNPWVNPGL 249
           +   I G+ L HP+F+            +   ++L     P S  G+EDP +N  V   L
Sbjct: 184 ENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV-VGSDL 243

Query: 250 GRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKA 306
             L C RVL++ A  D L      + A L +SGW G V+++ET+ E HVFHL +PD E A
Sbjct: 244 TGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENA 303

BLAST of Lag0009061 vs. ExPASy Swiss-Prot
Match: Q9SMM9 (Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1)

HSP 1 Score: 250.8 bits (639), Expect = 2.1e-65
Identity = 137/323 (42.41%), Postives = 198/323 (61.30%), Query Frame = 0

Query: 10  EVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQ-- 69
           E+  D+ P+  ++KSGR+ER      VPPS++P  GV SKDV+  P   LS+RIYLP+  
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 70  ---VPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPL 129
                +++ KLPLL+Y HGG F + +  SP YH  L +  + ++ VA+SV+YR APEHP+
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 130 PAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDN 189
           P +YDD+W A++W  SH    G+E WLN+HADF +VFL GDSAGANI++++  +A++   
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 190 GPKGLN---IIGLGLVHPFFMIDKP--DK---------LIEYIFP-TSAGLEDPKMNPWV 249
            P+ LN   I G+ LVHP+F    P  DK          IE ++   S   +D   +P++
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 250 N------PGLGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSV-EMVETEGEEHV 306
           N        L  L C +VL++ AE+D L  +  G+   L +S W G V ++VET+GE HV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

BLAST of Lag0009061 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 3.5e-65
Identity = 126/324 (38.89%), Postives = 203/324 (62.65%), Query Frame = 0

Query: 3   SADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPS-TDPLTGVQSKDVLIDPHTQLSV 62
           ++    +E+  +  P+ RV+K G VERF  +  VPPS  DP TGV +KD++I  +  +S 
Sbjct: 5   TSTTTSKEIDRELPPLLRVYKDGTVERFLGSSFVPPSPEDPETGVSTKDIVISENPTISA 64

Query: 63  RIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPE 122
           R+YLP++ ++T KLP+L+Y HGGAF + S  S ++ R+L  +A++AN + +S+EYRLAPE
Sbjct: 65  RVYLPKLNNTTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPE 124

Query: 123 HPLPAAYDDAWAAVEWAASHSNRD----GAETWLNEHADFDRVFLVGDSAGANISYNVVG 182
           HPLPAAY+D W A++W  SHS  +     A+ WL +H DF+R ++ GD++GANI++N   
Sbjct: 125 HPLPAAYEDGWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAAL 184

Query: 183 RASESDNGPKGLNIIGLGLVHPFFMIDK--------------PDKLIEYIFPTS-AGLED 242
           R   ++  P GL I G+    P F   K              P ++  +++P +  G+++
Sbjct: 185 RVG-AEALPGGLRIAGVLSAFPLFWGSKPVLSEPVEGHEKSSPMQVWNFVYPDAPGGIDN 244

Query: 243 PKMNPWV--NPGLGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEE 302
           P +NP     P L  L C ++L+  A +D L+DR   ++ A++ESGW+G VE+ + EGEE
Sbjct: 245 PLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEGEE 304

Query: 303 HVFHLFNPDCEKAVALVKKVASFI 305
           H F +++P+ E +  L+ ++ASF+
Sbjct: 305 HCFQIYHPETENSKDLIGRIASFL 327

BLAST of Lag0009061 vs. ExPASy TrEMBL
Match: A0A6J1L361 (probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=1)

HSP 1 Score: 538.9 bits (1387), Expect = 1.4e-149
Identity = 265/306 (86.60%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS   AGEEVVF F  +FRVFKSGRVER  PAD VPPSTD  TGVQSKDVLIDPHT+LS
Sbjct: 1   MDS--TAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP+V  S  KLPLLIYIHGGAF IGSVAS IYH HLISLAAEANAVA+SVEYRLAP
Sbjct: 61  VRIYLPKVSTSAEKLPLLIYIHGGAFTIGSVASSIYHSHLISLAAEANAVAVSVEYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           E+PLPAAYDDAW AVEW ASHSNRDGAE WLNEHADFD VF+VGDSAGANISYNVVGRAS
Sbjct: 121 ENPLPAAYDDAWTAVEWVASHSNRDGAEMWLNEHADFDCVFIVGDSAGANISYNVVGRAS 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
           E+DNGP+GLNIIGLGLVHPFFMIDKPDKLIEYIFPTS GLEDP+MNPW NPGL R RCNR
Sbjct: 181 ENDNGPEGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLARFRCNR 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           VLI+AAE DFL DRARGFHAALRESGW+GSVEMVE EGE+HVFHLFNPDCEKAVALVKKV
Sbjct: 241 VLIIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCEKAVALVKKV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 304

BLAST of Lag0009061 vs. ExPASy TrEMBL
Match: A0A6J1G929 (probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.4e-149
Identity = 265/306 (86.60%), Postives = 277/306 (90.52%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS   AGEEVVF F  +FRVFKSGRVER  PAD VPPSTD  TGVQSKDVLIDPHT+LS
Sbjct: 1   MDS--TAGEEVVFQFLSVFRVFKSGRVERLVPADTVPPSTDSRTGVQSKDVLIDPHTKLS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP+V  S  KLPLLIYIHGGAFAIGSVAS IYH HLISLAAEANAVA+SVEYRLAP
Sbjct: 61  VRIYLPKVSTSAKKLPLLIYIHGGAFAIGSVASSIYHSHLISLAAEANAVAVSVEYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           E+PLPAAYDDAW AVEW ASHSNRDGAE WLNEHADFDRVF+VGDSAGANISYNVVGRAS
Sbjct: 121 ENPLPAAYDDAWKAVEWVASHSNRDGAEMWLNEHADFDRVFIVGDSAGANISYNVVGRAS 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
           E+DNGP+GL IIGLGLVHPFFMIDKPDKLIEYIFPTS GLEDP+MNPW NPGLGR RCNR
Sbjct: 181 ENDNGPEGLKIIGLGLVHPFFMIDKPDKLIEYIFPTSTGLEDPRMNPWANPGLGRFRCNR 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           VLI+AAE DFL DRARGFHAALRESGW+GSVEMVE EGE+HVFHLFNPDC KAVALVK V
Sbjct: 241 VLIIAAEHDFLNDRARGFHAALRESGWKGSVEMVENEGEDHVFHLFNPDCGKAVALVKTV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 304

BLAST of Lag0009061 vs. ExPASy TrEMBL
Match: A0A6J1D5P6 (probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 SV=1)

HSP 1 Score: 496.9 bits (1278), Expect = 6.1e-137
Identity = 250/306 (81.70%), Postives = 269/306 (87.91%), Query Frame = 0

Query: 1   MDSADAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLS 60
           MDS DAA +EVVF+F P+FRVF+SGRVER  P DRVPPSTD  TGVQSKD+LIDP T +S
Sbjct: 1   MDS-DAA-DEVVFEFQPVFRVFRSGRVERLIPGDRVPPSTDQRTGVQSKDILIDPQTGVS 60

Query: 61  VRIYLPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAP 120
           VRIYLP VPDST KLPLLIYIHGGAFAIGS  S IYHRHLISLAAEANAVALSV YRLAP
Sbjct: 61  VRIYLPPVPDST-KLPLLIYIHGGAFAIGSADSAIYHRHLISLAAEANAVALSVNYRLAP 120

Query: 121 EHPLPAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRAS 180
           EHPLPAAYDDAW +VEWAASH+NRDGAE WLN HADFDRVFLVGDSAGANISYN+VGRA 
Sbjct: 121 EHPLPAAYDDAWTSVEWAASHANRDGAEIWLNNHADFDRVFLVGDSAGANISYNLVGRAG 180

Query: 181 ESDNGPKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNR 240
              NGP GLNI+GLGL+HPFFMIDKPDKLIEYIFPTSAGLEDP+MNP  NP L  LRC +
Sbjct: 181 ---NGPAGLNIVGLGLIHPFFMIDKPDKLIEYIFPTSAGLEDPRMNPRANPELRSLRCTQ 240

Query: 241 VLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKV 300
           V+IL AE D+LKDR RGFHAALRESGW+GSVE+VETEGE HVFHLFNP+ EKA  LVK+V
Sbjct: 241 VVILVAENDWLKDRGRGFHAALRESGWKGSVEIVETEGEGHVFHLFNPENEKAATLVKQV 300

Query: 301 ASFIQS 307
           ASFIQS
Sbjct: 301 ASFIQS 300

BLAST of Lag0009061 vs. ExPASy TrEMBL
Match: A0A314Y2B2 (Putative carboxylesterase 5 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn_20876 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 4.3e-98
Identity = 173/299 (57.86%), Postives = 226/299 (75.59%), Query Frame = 0

Query: 6   AAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYL 65
           ++  E+  +F  IFR++K GRVER    + VPPSTDP TGV+SKD+++ P + LS RI+L
Sbjct: 5   SSSTEISVEFPTIFRIYKDGRVERLKGTETVPPSTDPETGVRSKDIVLSPESGLSARIFL 64

Query: 66  PQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLP 125
           P++ D T KLPLL++IHGGAF I S  SP+YH H   L +EAN V LSV YR APEHPLP
Sbjct: 65  PKLTDPTRKLPLLVFIHGGAFVIESPYSPLYHNHAALLTSEANVVVLSVNYRRAPEHPLP 124

Query: 126 AAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNG 185
           AAY+D+W A++WAA+HSNR+G ETWLN++ DFDRVF+ GDSAGA ++++VV RA    +G
Sbjct: 125 AAYEDSWEALQWAAAHSNRNGPETWLNDYVDFDRVFVGGDSAGATLTHHVVHRA--GIHG 184

Query: 186 PKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNRVLILA 245
             G  I+G+ L HPFF   KPDKL+E +FP   GL+DP++NP  +P L  + C RVLI  
Sbjct: 185 LSGAKIVGMILFHPFFGNGKPDKLLEVVFPACNGLDDPRVNPGKDPKLREIGCGRVLIFV 244

Query: 246 AERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKVASFI 305
           AE+DFL+DRA  ++ AL++SGW G VE++E+EGE+HVFHLFNP C+KAV LVKKV SFI
Sbjct: 245 AEKDFLRDRAWAYYEALKKSGWGGMVEILESEGEDHVFHLFNPSCDKAVGLVKKVVSFI 301

BLAST of Lag0009061 vs. ExPASy TrEMBL
Match: A0A6P5TIN3 (probable carboxylesterase 12 OS=Prunus avium OX=42229 GN=LOC110767917 PE=4 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 4.3e-98
Identity = 173/299 (57.86%), Postives = 225/299 (75.25%), Query Frame = 0

Query: 6   AAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYL 65
           ++  E+  +F  IFR++K GRVER    + VPPSTD  TGV+SKDV++ P + LS RI+L
Sbjct: 5   SSSTEISVEFPTIFRIYKDGRVERLKGTETVPPSTDLETGVRSKDVVLSPESGLSARIFL 64

Query: 66  PQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLP 125
           P++PD T KLPLL++IHGGAF I S  SP+YH H   L +EAN V LSV YR APEHPLP
Sbjct: 65  PKLPDPTRKLPLLVFIHGGAFVIESPYSPLYHNHAALLTSEANVVVLSVNYRRAPEHPLP 124

Query: 126 AAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNG 185
            AY+D+W A++WAA+HSNR+G E WLN++ DFDRVF+ GDSAGA ++++VV RA    +G
Sbjct: 125 VAYEDSWEALQWAAAHSNRNGPEIWLNDYVDFDRVFVGGDSAGATLTHHVVHRAGV--HG 184

Query: 186 PKGLNIIGLGLVHPFFMIDKPDKLIEYIFPTSAGLEDPKMNPWVNPGLGRLRCNRVLILA 245
             G  I+G+ L HPFF  +KPDKL+E +FP   GLEDP++NP  +P LG + C RVLI  
Sbjct: 185 LSGAKIVGMILFHPFFGNEKPDKLLEVVFPACNGLEDPRVNPGKDPKLGEIGCGRVLIFV 244

Query: 246 AERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKAVALVKKVASFI 305
           AE+DFL+DRA  ++ AL++SGW G VE++E+EGE+HVFHLFNP C+KAV LVKKV  FI
Sbjct: 245 AEKDFLRDRAWAYYEALKKSGWGGMVEILESEGEDHVFHLFNPSCDKAVGLVKKVVCFI 301

BLAST of Lag0009061 vs. TAIR 10
Match: AT3G48690.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 260.8 bits (665), Expect = 1.4e-69
Identity = 139/319 (43.57%), Postives = 197/319 (61.76%), Query Frame = 0

Query: 10  EVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQ-- 69
           E+  D  P+ +++KSGR+ER      VPPS++P  GV SKDV+      LSVRIYLP+  
Sbjct: 4   EIAVDCSPLLKIYKSGRIERLMGEATVPPSSEPQNGVVSKDVVYSADNNLSVRIYLPEKA 63

Query: 70  VPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLPAA 129
             ++ +KLPLL+Y HGG F I +  SP YH  L +  + +N VA+SV+YR APEHP+   
Sbjct: 64  AAETDSKLPLLVYFHGGGFIIETAFSPTYHTFLTTSVSASNCVAVSVDYRRAPEHPISVP 123

Query: 130 YDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNGPK 189
           +DD+W A++W  +H    G E WLN+HADF RVFL GDSAGANI +++  RA++    P 
Sbjct: 124 FDDSWTALKWVFTHITGSGQEDWLNKHADFSRVFLSGDSAGANIVHHMAMRAAKEKLSP- 183

Query: 190 GLN---IIGLGLVHPFFM----IDKPDKLIEYI-----------FPTSA-GLEDPKMNPW 249
           GLN   I G+ L+HP+F     ID+ D   E +            P S  G +DP +N  
Sbjct: 184 GLNDTGISGIILLHPYFWSKTPIDEKDTKDETLRMKIEAFWMMASPNSKDGTDDPLLNVV 243

Query: 250 VNPG--LGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLF 306
            +    L  L C +VL++ AE+D L  +  G+ A L +SGW+G VE+VE+EGE+HVFHL 
Sbjct: 244 QSESVDLSGLGCGKVLVMVAEKDALVRQGWGYAAKLEKSGWKGEVEVVESEGEDHVFHLL 303

BLAST of Lag0009061 vs. TAIR 10
Match: AT1G47480.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 1.8e-69
Identity = 133/316 (42.09%), Postives = 198/316 (62.66%), Query Frame = 0

Query: 5   DAAGEEVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIY 64
           ++  ++V  +  P   V   G VER    +  PP  DP+TGV SKD++I+P T LS RIY
Sbjct: 2   ESTKKQVSLELLPWLVVHTDGTVERLAGTEVCPPGLDPITGVFSKDIIIEPKTGLSARIY 61

Query: 65  LPQVPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPL 124
            P       K+PL++Y HGGAF I S + P YH  L  +  +AN +A+SV YRLAPEHPL
Sbjct: 62  RPFSIQPGQKIPLMLYFHGGAFLISSTSFPSYHTSLNKIVNQANVIAVSVNYRLAPEHPL 121

Query: 125 PAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDN 184
           P AY+D+W A++   + +     E W+N++AD D +FLVGDSAGANIS+++  RA +SD 
Sbjct: 122 PTAYEDSWTALKNIQAIN-----EPWINDYADLDSLFLVGDSAGANISHHLAFRAKQSD- 181

Query: 185 GPKGLNIIGLGLVHPFFMIDKP--------------DKLIEYIFPTSAGLEDPKMNPWV- 244
             + L I G+G++HP+F   +P              D   E++ P+  G +DP +NP+  
Sbjct: 182 --QTLKIKGIGMIHPYFWGTQPIGAEIKDEARKQMVDGWWEFVCPSEKGSDDPWINPFAD 241

Query: 245 -NPGLGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNP 304
            +P LG L C RV+I  AE+D L +R + ++  L +S W+G VE++ET+ ++HVFH+F P
Sbjct: 242 GSPDLGGLGCERVMITVAEKDILNERGKMYYERLVKSEWKGKVEIMETKEKDHVFHIFEP 301

BLAST of Lag0009061 vs. TAIR 10
Match: AT1G19190.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 255.4 bits (651), Expect = 5.9e-68
Identity = 133/312 (42.63%), Postives = 185/312 (59.29%), Query Frame = 0

Query: 10  EVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQ-- 69
           E+ FD+ P FR+FK+G +ER  P   VPPS +P  GV SKD +  P   LS+RIYLPQ  
Sbjct: 4   EIAFDYSPRFRIFKNGGIERLVPETFVPPSLNPENGVVSKDAVYSPEKNLSLRIYLPQNS 63

Query: 70  -VPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLPA 129
                  K+PLL+Y HGG F + +  SPIYH  L S  +  + +A+SVEYR APEHP+P 
Sbjct: 64  VYETGEKKIPLLVYFHGGGFIMETAFSPIYHTFLTSAVSATDCIAVSVEYRRAPEHPIPT 123

Query: 130 AYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNGP 189
            Y+D+W A++W  +H  R G E WLN+HADF +VFL GDSAGANI++++  R  +    P
Sbjct: 124 LYEDSWDAIQWIFTHITRSGPEDWLNKHADFSKVFLAGDSAGANIAHHMAIRVDKEKLPP 183

Query: 190 KGLNIIGLGLVHPFFM------------IDKPDKLIEYIFPTSA-GLEDPKMNPWVNPGL 249
           +   I G+ L HP+F+            +   ++L     P S  G+EDP +N  V   L
Sbjct: 184 ENFKISGMILFHPYFLSKALIEEMEVEAMRYYERLWRIASPDSGNGVEDPWINV-VGSDL 243

Query: 250 GRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLFNPDCEKA 306
             L C RVL++ A  D L      + A L +SGW G V+++ET+ E HVFHL +PD E A
Sbjct: 244 TGLGCRRVLVMVAGNDVLARGGWSYVAELEKSGWIGKVKVMETKEEGHVFHLRDPDSENA 303

BLAST of Lag0009061 vs. TAIR 10
Match: AT3G48700.1 (carboxyesterase 13 )

HSP 1 Score: 250.8 bits (639), Expect = 1.5e-66
Identity = 137/323 (42.41%), Postives = 198/323 (61.30%), Query Frame = 0

Query: 10  EVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQ-- 69
           E+  D+ P+  ++KSGR+ER      VPPS++P  GV SKDV+  P   LS+RIYLP+  
Sbjct: 4   EIAADYSPMLIIYKSGRIERLVGETTVPPSSNPQNGVVSKDVVYSPDNNLSLRIYLPEKA 63

Query: 70  ---VPDSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPL 129
                +++ KLPLL+Y HGG F + +  SP YH  L +  + ++ VA+SV+YR APEHP+
Sbjct: 64  ATAETEASVKLPLLVYFHGGGFLVETAFSPTYHTFLTAAVSASDCVAVSVDYRRAPEHPI 123

Query: 130 PAAYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDN 189
           P +YDD+W A++W  SH    G+E WLN+HADF +VFL GDSAGANI++++  +A++   
Sbjct: 124 PTSYDDSWTALKWVFSHIAGSGSEDWLNKHADFSKVFLAGDSAGANITHHMTMKAAKDKL 183

Query: 190 GPKGLN---IIGLGLVHPFFMIDKP--DK---------LIEYIFP-TSAGLEDPKMNPWV 249
            P+ LN   I G+ LVHP+F    P  DK          IE ++   S   +D   +P++
Sbjct: 184 SPESLNESGISGIILVHPYFWSKTPVDDKETTDVAIRTWIESVWTLASPNSKDGSDDPFI 243

Query: 250 N------PGLGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSV-EMVETEGEEHV 306
           N        L  L C +VL++ AE+D L  +  G+   L +S W G V ++VET+GE HV
Sbjct: 244 NVVQSESVDLSGLGCGKVLVMVAEKDALVRQGWGYWEKLGKSRWNGEVLDVVETKGEGHV 303

BLAST of Lag0009061 vs. TAIR 10
Match: AT1G49660.1 (carboxyesterase 5 )

HSP 1 Score: 245.0 bits (624), Expect = 8.0e-65
Identity = 138/318 (43.40%), Postives = 188/318 (59.12%), Query Frame = 0

Query: 10  EVVFDFHPIFRVFKSGRVERFFPADRVPPSTDPLTGVQSKDVLIDPHTQLSVRIYLPQVP 69
           E+  +F P  R++K GRVER    D +P S DP   V SKDV+  P   LSVR++LP   
Sbjct: 4   EIASEFLPFCRIYKDGRVERLIGTDTIPASLDPTYDVVSKDVIYSPENNLSVRLFLPHKS 63

Query: 70  ---DSTNKLPLLIYIHGGAFAIGSVASPIYHRHLISLAAEANAVALSVEYRLAPEHPLPA 129
               + NKLPLLIYIHGGA+ I S  SP+YH +L  +   AN +A+SV+YR APE P+PA
Sbjct: 64  TKLTAGNKLPLLIYIHGGAWIIESPFSPLYHNYLTEVVKSANCLAVSVQYRRAPEDPVPA 123

Query: 130 AYDDAWAAVEWAASHSNRDGAETWLNEHADFDRVFLVGDSAGANISYNVVGRASESDNGP 189
           AY+D W+A++W  +HSN  G   W+N+HADF +VFL GDSAG NIS+++  +A +     
Sbjct: 124 AYEDVWSAIQWIFAHSNGSGPVDWINKHADFGKVFLGGDSAGGNISHHMAMKAGKEKK-- 183

Query: 190 KGLNIIGLGLVHPFFM----IDKPD-----------KLIEYI-FPTSA-GLEDPKMNPWV 249
             L I G+ +VHP F     +D+ D           ++ E I  P S  G +DP  N  V
Sbjct: 184 LDLKIKGIAVVHPAFWGTDPVDEYDVQDKETRSGIAEIWEKIASPNSVNGTDDPLFN--V 243

Query: 250 N---PGLGRLRCNRVLILAAERDFLKDRARGFHAALRESGWEGSVEMVETEGEEHVFHLF 305
           N        L C++VL+  A +D    +   + A L +  WEG+VE+VE EGE+HVFHL 
Sbjct: 244 NGSGSDFSGLGCDKVLVAVAGKDVFVRQGLAYAAKLEKCEWEGTVEVVEEEGEDHVFHLQ 303

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6605131.12.6e-15087.25putative carboxylesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_023532126.13.4e-15086.93probable carboxylesterase 2 [Cucurbita pepo subsp. pepo][more]
KAG7035128.15.8e-15086.93putative carboxylesterase 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023007510.12.9e-14986.60probable carboxylesterase 2 [Cucurbita maxima][more]
XP_022948164.14.9e-14986.60probable carboxylesterase 2 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9SMN02.0e-6843.57Probable carboxylesterase 12 OS=Arabidopsis thaliana OX=3702 GN=CXE12 PE=1 SV=1[more]
Q9SX782.6e-6842.09Probable carboxylesterase 2 OS=Arabidopsis thaliana OX=3702 GN=CXE2 PE=2 SV=1[more]
Q9LMA78.3e-6742.63Probable carboxylesterase 1 OS=Arabidopsis thaliana OX=3702 GN=CXE1 PE=2 SV=1[more]
Q9SMM92.1e-6542.41Probable carboxylesterase 13 OS=Arabidopsis thaliana OX=3702 GN=CXE13 PE=1 SV=1[more]
Q5NUF43.5e-6538.892-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1L3611.4e-14986.60probable carboxylesterase 2 OS=Cucurbita maxima OX=3661 GN=LOC111499978 PE=4 SV=... [more]
A0A6J1G9292.4e-14986.60probable carboxylesterase 2 OS=Cucurbita moschata OX=3662 GN=LOC111451823 PE=4 S... [more]
A0A6J1D5P66.1e-13781.70probable carboxylesterase 2 OS=Momordica charantia OX=3673 GN=LOC111017169 PE=3 ... [more]
A0A314Y2B24.3e-9857.86Putative carboxylesterase 5 OS=Prunus yedoensis var. nudiflora OX=2094558 GN=Pyn... [more]
A0A6P5TIN34.3e-9857.86probable carboxylesterase 12 OS=Prunus avium OX=42229 GN=LOC110767917 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48690.11.4e-6943.57alpha/beta-Hydrolases superfamily protein [more]
AT1G47480.11.8e-6942.09alpha/beta-Hydrolases superfamily protein [more]
AT1G19190.15.9e-6842.63alpha/beta-Hydrolases superfamily protein [more]
AT3G48700.11.5e-6642.41carboxyesterase 13 [more]
AT1G49660.18.0e-6543.40carboxyesterase 5 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 78..284
e-value: 1.7E-44
score: 152.2
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 22..307
e-value: 1.6E-71
score: 243.4
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 11..305
NoneNo IPR availablePANTHERPTHR23024:SF414CARBOXYLESTERASE 13-RELATEDcoord: 7..304
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 7..304

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009061.1Lag0009061.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0052689 carboxylic ester hydrolase activity
molecular_function GO:0016787 hydrolase activity