Lag0008747 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0008747
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionS-locus lectin protein kinase family protein, putative
Locationchr9: 29125385 .. 29126026 (-)
RNA-Seq ExpressionLag0008747
SyntenyLag0008747
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACAGAAGGATCCACTACTTTCTTTGTTAGGGGTAAAATGATCCCTTTCGACTTGGCCTCTATTAACCAATTCTATGGCCTTCCCAATACTGATAGGGATGGGTACAACGATTATGCAAGTACTCACATTAATGCCCATCAAATTATAGAGCATCTATGTAGGCCAGGGGCAACTTGGTTAATAAGAAGGGGTGAAGCAATAAACTTTAAATCCTCTGATCTGATAGTGGATAACAGAGCATGTCATAGTTTTCTGTGTGCCAAACTCTTACCCGTGATGCATCTGAGTGATGTAACAAAGGAAAGAGCAGCTCTGTTATTCTCTATAGCGACCAACCGTAGTGTAGACGTAGGAAAAGTTATATATTCATCCATGCGCAAGATTCGTCGAGGAACGACCACCGTAGGTCTAGGGCACCCTTCCTTCATCATAGCACTCTATAGAGCCACTGAGGTCGTGTGGGACCCCCGTGAGGAGATTAACCATCCTGCAGTTGCGATTGATGTGAACTTCATCACGATTAGACTTAGGGAGCCTGGACCTAGGATAACCCACCCACCACCTCCACCCCAACAGGAAGAACTAGAAGAGGAGCCCCAAGCTCAGGGAGAGCAACCCCCTTCGATCATCCTTCTCTAA

mRNA sequence

ATGACAGAAGGATCCACTACTTTCTTTGTTAGGGGTAAAATGATCCCTTTCGACTTGGCCTCTATTAACCAATTCTATGGCCTTCCCAATACTGATAGGGATGGGTACAACGATTATGCAAGTACTCACATTAATGCCCATCAAATTATAGAGCATCTATGTAGGCCAGGGGCAACTTGGTTAATAAGAAGGGGTGAAGCAATAAACTTTAAATCCTCTGATCTGATAGTGGATAACAGAGCATGTCATAGTTTTCTGTGTGCCAAACTCTTACCCGTGATGCATCTGAGTGATGTAACAAAGGAAAGAGCAGCTCTGTTATTCTCTATAGCGACCAACCGTAGTGTAGACGTAGGAAAAGTTATATATTCATCCATGCGCAAGATTCGTCGAGGAACGACCACCGTAGGTCTAGGGCACCCTTCCTTCATCATAGCACTCTATAGAGCCACTGAGGTCGTGTGGGACCCCCGTGAGGAGATTAACCATCCTGCAGTTGCGATTGATGTGAACTTCATCACGATTAGACTTAGGGAGCCTGGACCTAGGATAACCCACCCACCACCTCCACCCCAACAGGAAGAACTAGAAGAGGAGCCCCAAGCTCAGGGAGAGCAACCCCCTTCGATCATCCTTCTCTAA

Coding sequence (CDS)

ATGACAGAAGGATCCACTACTTTCTTTGTTAGGGGTAAAATGATCCCTTTCGACTTGGCCTCTATTAACCAATTCTATGGCCTTCCCAATACTGATAGGGATGGGTACAACGATTATGCAAGTACTCACATTAATGCCCATCAAATTATAGAGCATCTATGTAGGCCAGGGGCAACTTGGTTAATAAGAAGGGGTGAAGCAATAAACTTTAAATCCTCTGATCTGATAGTGGATAACAGAGCATGTCATAGTTTTCTGTGTGCCAAACTCTTACCCGTGATGCATCTGAGTGATGTAACAAAGGAAAGAGCAGCTCTGTTATTCTCTATAGCGACCAACCGTAGTGTAGACGTAGGAAAAGTTATATATTCATCCATGCGCAAGATTCGTCGAGGAACGACCACCGTAGGTCTAGGGCACCCTTCCTTCATCATAGCACTCTATAGAGCCACTGAGGTCGTGTGGGACCCCCGTGAGGAGATTAACCATCCTGCAGTTGCGATTGATGTGAACTTCATCACGATTAGACTTAGGGAGCCTGGACCTAGGATAACCCACCCACCACCTCCACCCCAACAGGAAGAACTAGAAGAGGAGCCCCAAGCTCAGGGAGAGCAACCCCCTTCGATCATCCTTCTCTAA

Protein sequence

MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATWLIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGKVIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFITIRLREPGPRITHPPPPPQQEELEEEPQAQGEQPPSIILL
Homology
BLAST of Lag0008747 vs. NCBI nr
Match: KAA0033353.1 (putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa] >TYJ96637.1 putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa])

HSP 1 Score: 202.2 bits (513), Expect = 4.4e-48
Identity = 98/173 (56.65%), Postives = 121/173 (69.94%), Query Frame = 0

Query: 1   MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATW 60
           M EGS+  FVRG+ + FD  +IN++Y LPN +RD Y  YAS H++ HQII  LC+PGA W
Sbjct: 89  MVEGSSRSFVRGRQVSFDYGTINRYYHLPNFERDEYAIYASEHVDVHQIIRELCQPGAEW 148

Query: 61  LIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGK 120
           +I  GE I FKSS+L V N+  H F+CAKLLPV H S VTKERA LL++IAT RSVDVGK
Sbjct: 149 IINPGEPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGK 208

Query: 121 VIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFI 174
           VI+ S+  IR+   T GLGH S I AL R   VVW+ +EE+  P   +D NFI
Sbjct: 209 VIHKSLCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFI 261

BLAST of Lag0008747 vs. NCBI nr
Match: XP_008458668.1 (PREDICTED: uncharacterized protein LOC103497996 [Cucumis melo])

HSP 1 Score: 202.2 bits (513), Expect = 4.4e-48
Identity = 98/173 (56.65%), Postives = 121/173 (69.94%), Query Frame = 0

Query: 1   MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATW 60
           M EGS+  FVRG+ + FD  +IN++Y LPN +RD Y  YAS H++ HQII  LC+PGA W
Sbjct: 27  MVEGSSRSFVRGRQVSFDYGTINRYYHLPNFERDEYAIYASEHVDVHQIIRELCQPGAEW 86

Query: 61  LIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGK 120
           +I  GE I FKSS+L V N+  H F+CAKLLPV H S VTKERA LL++IAT RSVDVGK
Sbjct: 87  IINPGEPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGK 146

Query: 121 VIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFI 174
           VI+ S+  IR+   T GLGH S I AL R   VVW+ +EE+  P   +D NFI
Sbjct: 147 VIHKSLCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFI 199

BLAST of Lag0008747 vs. NCBI nr
Match: KGN46897.1 (hypothetical protein Csa_020731 [Cucumis sativus])

HSP 1 Score: 199.9 bits (507), Expect = 2.2e-47
Identity = 97/173 (56.07%), Postives = 121/173 (69.94%), Query Frame = 0

Query: 1   MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATW 60
           M EGS+  FVRG+ + FD  +IN++Y LPN +RD Y+ YAS H++ HQII  LC+PGA W
Sbjct: 89  MVEGSSRSFVRGRQVSFDYGTINRYYHLPNFERDEYDIYASEHVDVHQIIRELCQPGAEW 148

Query: 61  LIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGK 120
           +I  GE I FKSS+L V N+  H F+CAKLLPV H S VTKERA LL++IAT RSVDVGK
Sbjct: 149 VINPGEPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGK 208

Query: 121 VIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFI 174
           VI  S+  IR+   T GLGH S I AL R   VVW+ +EE+  P   +D +FI
Sbjct: 209 VIQKSLCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKSFI 261

BLAST of Lag0008747 vs. NCBI nr
Match: EOY08969.1 (Uncharacterized protein TCM_024271 [Theobroma cacao])

HSP 1 Score: 116.7 bits (291), Expect = 2.5e-22
Identity = 56/155 (36.13%), Postives = 91/155 (58.71%), Query Frame = 0

Query: 12  GKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATWLIRRGEAINFK 71
           G  +PF+  +INQFY   + + D Y+ + +  IN  +++  L   G  W + +G  I+FK
Sbjct: 14  GTKVPFNAHTINQFYNTLDIENDEYDQFVNGDINLDEVLRSLSILGTEWQVHKGVVISFK 73

Query: 72  SSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGKVIYSSMRKIRR 131
           ++ +  D +  + F+  KLL V +LSDVTK+RA LL++I T + +D+G++I+ ++    R
Sbjct: 74  ANAMDNDYKVWYHFVAMKLLLVKYLSDVTKDRAILLYAIVTKKFIDIGQLIFKNIIMSAR 133

Query: 132 GTTTVGLGHPSFIIALYRATEVVWDPREEINHPAV 167
                GL +PS I AL     VVW P EE+ HP +
Sbjct: 134 SPPN-GLWYPSLITALCCQARVVWSPNEELPHPKI 167

BLAST of Lag0008747 vs. NCBI nr
Match: EOY12720.1 (S-locus lectin protein kinase family protein, putative [Theobroma cacao])

HSP 1 Score: 115.2 bits (287), Expect = 7.1e-22
Identity = 58/161 (36.02%), Postives = 91/161 (56.52%), Query Frame = 0

Query: 9    FVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATWLIRRGEAI 68
            F RGK +PFD  +INQF  +P  + D Y  Y   ++N  ++I  L  PG  W I +G ++
Sbjct: 961  FCRGKKVPFDSFTINQFSNIPKIENDEYAHYTDGNVNLDEVITFLYDPGTQWKISKGISV 1020

Query: 69   NFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGKVIYSSMRK 128
            +FK++ L    +  +  L AK+ P+  LSDVTK+RA LL+++ T +S++VGK I++S+  
Sbjct: 1021 SFKANTLDKFFKIWYHILTAKMFPIKDLSDVTKDRAILLYAMVTGKSINVGKQIFNSIVH 1080

Query: 129  IRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAID 170
                     + + S IIAL +   V W   EE+ H    +D
Sbjct: 1081 CAISARD-NIWYLSLIIALCKQARVQWSSEEELLHLRAPLD 1120

BLAST of Lag0008747 vs. ExPASy TrEMBL
Match: A0A5D3BBY3 (Putative S-locus lectin protein kinase family protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold26G00020 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 2.1e-48
Identity = 98/173 (56.65%), Postives = 121/173 (69.94%), Query Frame = 0

Query: 1   MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATW 60
           M EGS+  FVRG+ + FD  +IN++Y LPN +RD Y  YAS H++ HQII  LC+PGA W
Sbjct: 89  MVEGSSRSFVRGRQVSFDYGTINRYYHLPNFERDEYAIYASEHVDVHQIIRELCQPGAEW 148

Query: 61  LIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGK 120
           +I  GE I FKSS+L V N+  H F+CAKLLPV H S VTKERA LL++IAT RSVDVGK
Sbjct: 149 IINPGEPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGK 208

Query: 121 VIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFI 174
           VI+ S+  IR+   T GLGH S I AL R   VVW+ +EE+  P   +D NFI
Sbjct: 209 VIHKSLCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFI 261

BLAST of Lag0008747 vs. ExPASy TrEMBL
Match: A0A1S3C7Y0 (uncharacterized protein LOC103497996 OS=Cucumis melo OX=3656 GN=LOC103497996 PE=4 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 2.1e-48
Identity = 98/173 (56.65%), Postives = 121/173 (69.94%), Query Frame = 0

Query: 1   MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATW 60
           M EGS+  FVRG+ + FD  +IN++Y LPN +RD Y  YAS H++ HQII  LC+PGA W
Sbjct: 27  MVEGSSRSFVRGRQVSFDYGTINRYYHLPNFERDEYAIYASEHVDVHQIIRELCQPGAEW 86

Query: 61  LIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGK 120
           +I  GE I FKSS+L V N+  H F+CAKLLPV H S VTKERA LL++IAT RSVDVGK
Sbjct: 87  IINPGEPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGK 146

Query: 121 VIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFI 174
           VI+ S+  IR+   T GLGH S I AL R   VVW+ +EE+  P   +D NFI
Sbjct: 147 VIHKSLCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKNFI 199

BLAST of Lag0008747 vs. ExPASy TrEMBL
Match: A0A0A0KER1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149380 PE=4 SV=1)

HSP 1 Score: 199.9 bits (507), Expect = 1.1e-47
Identity = 97/173 (56.07%), Postives = 121/173 (69.94%), Query Frame = 0

Query: 1   MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATW 60
           M EGS+  FVRG+ + FD  +IN++Y LPN +RD Y+ YAS H++ HQII  LC+PGA W
Sbjct: 89  MVEGSSRSFVRGRQVSFDYGTINRYYHLPNFERDEYDIYASEHVDVHQIIRELCQPGAEW 148

Query: 61  LIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGK 120
           +I  GE I FKSS+L V N+  H F+CAKLLPV H S VTKERA LL++IAT RSVDVGK
Sbjct: 149 VINPGEPIRFKSSNLTVSNQVWHKFICAKLLPVAHTSSVTKERAILLYAIATKRSVDVGK 208

Query: 121 VIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFI 174
           VI  S+  IR+   T GLGH S I AL R   VVW+ +EE+  P   +D +FI
Sbjct: 209 VIQKSLCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKSFI 261

BLAST of Lag0008747 vs. ExPASy TrEMBL
Match: A0A0A0KNI1 (AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G468460 PE=4 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 9.7e-33
Identity = 80/173 (46.24%), Postives = 101/173 (58.38%), Query Frame = 0

Query: 1   MTEGSTTFFVRGKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATW 60
           M EGS+  FVRG+ + FD  +IN++Y LPN +RD Y+ YAS H++ HQII  LC+PGA W
Sbjct: 89  MVEGSSRSFVRGRQVSFDYGTINRYYHLPNFERDEYDIYASEHVDVHQIIRELCQPGAEW 148

Query: 61  LIRRGEAINFKSSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGK 120
                                        LLP+ H S VTKERA LL++IAT RSVDVGK
Sbjct: 149 -----------------------------LLPMAHTSSVTKERAILLYAIATKRSVDVGK 208

Query: 121 VIYSSMRKIRRGTTTVGLGHPSFIIALYRATEVVWDPREEINHPAVAIDVNFI 174
           VI  S+  IR+   T GLGH S I AL R   VVW+ +EE+  P   +D +FI
Sbjct: 209 VIQKSLCNIRKSGMTGGLGHSSLITALCRNEGVVWNEKEELVDPKPIMDKSFI 232

BLAST of Lag0008747 vs. ExPASy TrEMBL
Match: A0A061F366 (Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_024271 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 1.2e-22
Identity = 56/155 (36.13%), Postives = 91/155 (58.71%), Query Frame = 0

Query: 12  GKMIPFDLASINQFYGLPNTDRDGYNDYASTHINAHQIIEHLCRPGATWLIRRGEAINFK 71
           G  +PF+  +INQFY   + + D Y+ + +  IN  +++  L   G  W + +G  I+FK
Sbjct: 14  GTKVPFNAHTINQFYNTLDIENDEYDQFVNGDINLDEVLRSLSILGTEWQVHKGVVISFK 73

Query: 72  SSDLIVDNRACHSFLCAKLLPVMHLSDVTKERAALLFSIATNRSVDVGKVIYSSMRKIRR 131
           ++ +  D +  + F+  KLL V +LSDVTK+RA LL++I T + +D+G++I+ ++    R
Sbjct: 74  ANAMDNDYKVWYHFVAMKLLLVKYLSDVTKDRAILLYAIVTKKFIDIGQLIFKNIIMSAR 133

Query: 132 GTTTVGLGHPSFIIALYRATEVVWDPREEINHPAV 167
                GL +PS I AL     VVW P EE+ HP +
Sbjct: 134 SPPN-GLWYPSLITALCCQARVVWSPNEELPHPKI 167

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0033353.14.4e-4856.65putative S-locus lectin protein kinase family protein [Cucumis melo var. makuwa]... [more]
XP_008458668.14.4e-4856.65PREDICTED: uncharacterized protein LOC103497996 [Cucumis melo][more]
KGN46897.12.2e-4756.07hypothetical protein Csa_020731 [Cucumis sativus][more]
EOY08969.12.5e-2236.13Uncharacterized protein TCM_024271 [Theobroma cacao][more]
EOY12720.17.1e-2236.02S-locus lectin protein kinase family protein, putative [Theobroma cacao][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5D3BBY32.1e-4856.65Putative S-locus lectin protein kinase family protein OS=Cucumis melo var. makuw... [more]
A0A1S3C7Y02.1e-4856.65uncharacterized protein LOC103497996 OS=Cucumis melo OX=3656 GN=LOC103497996 PE=... [more]
A0A0A0KER11.1e-4756.07Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G149380 PE=4 SV=1[more]
A0A0A0KNI19.7e-3346.24AA_kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G468460 P... [more]
A0A061F3661.2e-2236.13Uncharacterized protein OS=Theobroma cacao OX=3641 GN=TCM_024271 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 179..213

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0008747.1Lag0008747.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006561 proline biosynthetic process
cellular_component GO:0005829 cytosol
molecular_function GO:0005488 binding
molecular_function GO:0004349 glutamate 5-kinase activity